mRNA_M-pyrifera_M_contig106020.1295.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig106020.1295.1
Unique NamemRNA_M-pyrifera_M_contig106020.1295.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig106020.1295.1 vs. uniprot
Match: R7QQL7_CHOCR (Putative ATP dependent DNA ligase n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QQL7_CHOCR)

HSP 1 Score: 268 bits (685), Expect = 3.420e-85
Identity = 124/154 (80.52%), Postives = 140/154 (90.91%), Query Frame = 1 
Query:    1 MEGLMIKDLSSSYVSGGPKHAWYKWKYDPHLLDAVLMYAQRGHGKRSSYYSDYTFGLWAGDGQLLPIGKAYFGFTDEELKQLDTWIRNHKLQRFGPVQEVEKELVFEVAYDSAQTSARHKSGYALRFPRISRIRWDKPASEANQLEDLAKLVDR 462
            +EGLMIKD+SS YVSG PKHAWYKWK DP LLDAVLMYA+RG GKRSS+YSDYTFGLW  DG LLPIGKAYFGFTDEELK++D W+R+HKLQRFGPVQEVEKELVFEVA+D+AQ S RHKSGYALRFPRI RIRWDKPA+EA+++ +LAKLV +
Sbjct:  381 IEGLMIKDMSSVYVSGRPKHAWYKWKRDPFLLDAVLMYARRGTGKRSSFYSDYTFGLWGEDGTLLPIGKAYFGFTDEELKKIDNWVRSHKLQRFGPVQEVEKELVFEVAFDAAQISKRHKSGYALRFPRIHRIRWDKPAAEADKIVNLAKLVGK 534
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BLAST of mRNA_M-pyrifera_M_contig106020.1295.1 vs. uniprot
Match: UPI0003F83C72 (cisplatin damage response ATP-dependent DNA ligase n=1 Tax=Hellea balneolensis TaxID=287478 RepID=UPI0003F83C72)

HSP 1 Score: 266 bits (680), Expect = 1.690e-84
Identity = 124/152 (81.58%), Postives = 136/152 (89.47%), Query Frame = 1 
Query:    1 MEGLMIKDLSSSYVSGGPKHAWYKWKYDPHLLDAVLMYAQRGHGKRSSYYSDYTFGLWAGDGQLLPIGKAYFGFTDEELKQLDTWIRNHKLQRFGPVQEVEKELVFEVAYDSAQTSARHKSGYALRFPRISRIRWDKPASEANQLEDLAKLV 456
            +EGLMIKD+SS YVSG PKHAWYKWK DP LLDAVLMYA+RG GKRSSYYSDYTFGLW  DG+LLPIGKAYFGFTDEELK++D W+RNHKLQRFGPVQEVEK LVFEVA+DSAQ S RHKSGYALRFPRI RIRWDKPA+EA++L  L  L+
Sbjct:  376 IEGLMIKDVSSIYVSGRPKHAWYKWKRDPFLLDAVLMYARRGTGKRSSYYSDYTFGLWNEDGELLPIGKAYFGFTDEELKEIDKWVRNHKLQRFGPVQEVEKGLVFEVAFDSAQESKRHKSGYALRFPRIHRIRWDKPAAEADKLSSLEALI 527
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BLAST of mRNA_M-pyrifera_M_contig106020.1295.1 vs. uniprot
Match: UPI0016775EE6 (cisplatin damage response ATP-dependent DNA ligase n=1 Tax=Litorimonas cladophorae TaxID=1220491 RepID=UPI0016775EE6)

HSP 1 Score: 260 bits (664), Expect = 4.790e-82
Identity = 121/152 (79.61%), Postives = 135/152 (88.82%), Query Frame = 1 
Query:    1 MEGLMIKDLSSSYVSGGPKHAWYKWKYDPHLLDAVLMYAQRGHGKRSSYYSDYTFGLWAGDGQLLPIGKAYFGFTDEELKQLDTWIRNHKLQRFGPVQEVEKELVFEVAYDSAQTSARHKSGYALRFPRISRIRWDKPASEANQLEDLAKLV 456
            +EGLMIKD+SS Y SG PKHAWYKWK DP LLDAVLMYA+RG GKRSS+YSDYTFGLW+ D  LLPIGKAYFGFTDEELK++D W+R +KLQRFGPVQEVEK LVFEVA+D+AQ S RHKSGYALRFPRI RIRWDKPA EA+++E LAKLV
Sbjct:  381 IEGLMIKDVSSVYASGRPKHAWYKWKRDPFLLDAVLMYARRGTGKRSSFYSDYTFGLWSDDDTLLPIGKAYFGFTDEELKKIDNWVRANKLQRFGPVQEVEKALVFEVAFDAAQVSTRHKSGYALRFPRIHRIRWDKPADEADRIETLAKLV 532
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BLAST of mRNA_M-pyrifera_M_contig106020.1295.1 vs. uniprot
Match: UPI000378BA48 (cisplatin damage response ATP-dependent DNA ligase n=1 Tax=Robiginitomaculum antarcticum TaxID=437507 RepID=UPI000378BA48)

HSP 1 Score: 259 bits (662), Expect = 8.200e-82
Identity = 122/152 (80.26%), Postives = 133/152 (87.50%), Query Frame = 1 
Query:    1 MEGLMIKDLSSSYVSGGPKHAWYKWKYDPHLLDAVLMYAQRGHGKRSSYYSDYTFGLWAGDGQLLPIGKAYFGFTDEELKQLDTWIRNHKLQRFGPVQEVEKELVFEVAYDSAQTSARHKSGYALRFPRISRIRWDKPASEANQLEDLAKLV 456
            +EGLMIKD SS YV G PKH WYKWK DP LLDAVLMYAQRG GKRSSYYSDYTFGLW  + +LLPIGKAYFGFTDEELK++D WIRNHKL RFGPVQEVEKELVFEVA+DSA  SARHKSGYALRFPRI RIRWDKPA+EA++L  +A L+
Sbjct:  375 IEGLMIKDKSSLYVPGRPKHGWYKWKRDPFLLDAVLMYAQRGTGKRSSYYSDYTFGLWNEEDKLLPIGKAYFGFTDEELKEIDKWIRNHKLARFGPVQEVEKELVFEVAFDSAHESARHKSGYALRFPRIHRIRWDKPAAEADRLTAIAALI 526
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BLAST of mRNA_M-pyrifera_M_contig106020.1295.1 vs. uniprot
Match: A0A8J3G1E1_9PROT (DNA ligase (ATP) n=1 Tax=Algimonas arctica TaxID=1479486 RepID=A0A8J3G1E1_9PROT)

HSP 1 Score: 255 bits (652), Expect = 2.990e-80
Identity = 121/156 (77.56%), Postives = 135/156 (86.54%), Query Frame = 1 
Query:    4 EGLMIKDLSSSYVSGGPKHAWYKWKYDPHLLDAVLMYAQRGHGKRSSYYSDYTFGLWA---GDGQLLPIGKAYFGFTDEELKQLDTWIRNHKLQRFGPVQEVEKELVFEVAYDSAQTSARHKSGYALRFPRISRIRWDKPASEANQLEDLAKLVDR 462
            EGLM+K   + YVSG PKHAWYKWK DP LLDAVLMYAQRG GKRSSYYSDYTFGLWA   G+ +LLPIGKAYFGFTDEELKQLD+WIRNHK+ R+GPVQEV+KELVFEVA+DSA  S RHKSGYALRFPRISRIRWDKPA EA++LE +  L+ +
Sbjct:  379 EGLMVKRRDTPYVSGRPKHAWYKWKRDPFLLDAVLMYAQRGTGKRSSYYSDYTFGLWANVEGEAKLLPIGKAYFGFTDEELKQLDSWIRNHKVARYGPVQEVDKELVFEVAFDSAHESPRHKSGYALRFPRISRIRWDKPAHEADRLEIMDTLIGK 534
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BLAST of mRNA_M-pyrifera_M_contig106020.1295.1 vs. uniprot
Match: A0A378LQJ9_9GAMM (DNA ligase (ATP) n=1 Tax=Legionella wadsworthii TaxID=28088 RepID=A0A378LQJ9_9GAMM)

HSP 1 Score: 243 bits (620), Expect = 1.490e-75
Identity = 112/152 (73.68%), Postives = 128/152 (84.21%), Query Frame = 1 
Query:    1 MEGLMIKDLSSSYVSGGPKHAWYKWKYDPHLLDAVLMYAQRGHGKRSSYYSDYTFGLWAGDGQLLPIGKAYFGFTDEELKQLDTWIRNHKLQRFGPVQEVEKELVFEVAYDSAQTSARHKSGYALRFPRISRIRWDKPASEANQLEDLAKLV 456
            +EG+M+K  SS Y++G PK  WYKWK DPHL+DA+LMYAQRGHGKRSSYYSDYTFGLW+   QLLPIGKAYFGFTDEEL QLD WIR H L  FGPV+EV+KELV E+A+D+  TS RHKSG ALRFPRI+RIRWDKP  EA+QLEDL KL+
Sbjct:  372 VEGIMLKKNSSLYLAGRPKGCWYKWKKDPHLIDAILMYAQRGHGKRSSYYSDYTFGLWS-QNQLLPIGKAYFGFTDEELYQLDKWIRYHTLNHFGPVREVQKELVLEIAFDAVNTSNRHKSGVALRFPRINRIRWDKPPEEADQLEDLKKLI 522
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BLAST of mRNA_M-pyrifera_M_contig106020.1295.1 vs. uniprot
Match: A0A378J159_9GAMM (DNA ligase (ATP) n=2 Tax=Legionella TaxID=445 RepID=A0A378J159_9GAMM)

HSP 1 Score: 240 bits (612), Expect = 2.230e-74
Identity = 111/152 (73.03%), Postives = 132/152 (86.84%), Query Frame = 1 
Query:    1 MEGLMIKDLSSSYVSGGPKHAWYKWKYDPHLLDAVLMYAQRGHGKRSSYYSDYTFGLWAGDGQLLPIGKAYFGFTDEELKQLDTWIRNHKLQRFGPVQEVEKELVFEVAYDSAQTSARHKSGYALRFPRISRIRWDKPASEANQLEDLAKLV 456
            +EGLMIK+  S Y++G P   WYKWK DP ++DAVLMYAQRGHGKRSSYYSDYTFGLW  +G+LLPIGKAYFGFTDEEL+QLD WIRN+ + RFGPV+EV+KELVFEVA+D+   S+RHKSG ALRFPRI+RIRWDKPA EA+QL+ L+KL+
Sbjct:  373 VEGLMIKNKQSPYLAGRPMGHWYKWKRDPLIIDAVLMYAQRGHGKRSSYYSDYTFGLWQ-EGELLPIGKAYFGFTDEELRQLDQWIRNNTIHRFGPVREVKKELVFEVAFDAVNLSSRHKSGLALRFPRINRIRWDKPAEEADQLKTLSKLM 523
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BLAST of mRNA_M-pyrifera_M_contig106020.1295.1 vs. uniprot
Match: A0A7G4RF43_9GAMM (DNA ligase (ATP) n=1 Tax=Legionella sp. PC997 TaxID=2755562 RepID=A0A7G4RF43_9GAMM)

HSP 1 Score: 239 bits (611), Expect = 3.140e-74
Identity = 112/152 (73.68%), Postives = 129/152 (84.87%), Query Frame = 1 
Query:    1 MEGLMIKDLSSSYVSGGPKHAWYKWKYDPHLLDAVLMYAQRGHGKRSSYYSDYTFGLWAGDGQLLPIGKAYFGFTDEELKQLDTWIRNHKLQRFGPVQEVEKELVFEVAYDSAQTSARHKSGYALRFPRISRIRWDKPASEANQLEDLAKLV 456
            +EGLM+K  SS Y++G PK  WYKWK DP+++DA+LMYAQRGHGKRSSYYSDYTFGLW  + QLLPIGKAYFGFTDEEL QLD WIR+H L RFGPV+EVEKELV E+A+D+   S RHKSG ALRFPRI+RIRWDKPA EA+QLE L KL+
Sbjct:  372 VEGLMLKKKSSLYLAGRPKGCWYKWKKDPYIIDAILMYAQRGHGKRSSYYSDYTFGLWKLN-QLLPIGKAYFGFTDEELYQLDKWIRSHTLNRFGPVREVEKELVLEIAFDAVHWSNRHKSGVALRFPRINRIRWDKPAHEADQLETLKKLI 522
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BLAST of mRNA_M-pyrifera_M_contig106020.1295.1 vs. uniprot
Match: A0A4Q3JHD8_9HYPH (DNA ligase (ATP) (Fragment) n=1 Tax=Hyphomicrobiales bacterium TaxID=1909294 RepID=A0A4Q3JHD8_9HYPH)

HSP 1 Score: 231 bits (588), Expect = 7.150e-74
Identity = 105/147 (71.43%), Postives = 127/147 (86.39%), Query Frame = 1 
Query:    4 EGLMIKDLSSSYVSGGPKHAWYKWKYDPHLLDAVLMYAQRGHGKRSSYYSDYTFGLWAGDGQLLPIGKAYFGFTDEELKQLDTWIRNHKLQRFGPVQEVEKELVFEVAYDSAQTSARHKSGYALRFPRISRIRWDKPASEANQLEDL 444
            EG+M+K  +S YV G PK  WYKWK DP+++DA+LMYAQRGHGKRSS+YSDYTFG+W G+ +++PIGKAYFGFTDEELKQLD W+RN+ +  FGPV+EV+KELVFEVA+DSAQ S RHKSG ALRFPRI+RIRWDKPA EA  L+D+
Sbjct:  115 EGVMVKLRTSPYVPGRPKGLWYKWKRDPNVVDAILMYAQRGHGKRSSFYSDYTFGVWKGN-EIVPIGKAYFGFTDEELKQLDKWVRNNTVASFGPVREVKKELVFEVAFDSAQESTRHKSGVALRFPRINRIRWDKPAGEAATLQDM 260
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BLAST of mRNA_M-pyrifera_M_contig106020.1295.1 vs. uniprot
Match: UPI001BDF211E (cisplatin damage response ATP-dependent DNA ligase n=1 Tax=Glaciecola sp. XM2 TaxID=1914931 RepID=UPI001BDF211E)

HSP 1 Score: 232 bits (592), Expect = 8.540e-74
Identity = 107/152 (70.39%), Postives = 126/152 (82.89%), Query Frame = 1 
Query:    1 MEGLMIKDLSSSYVSGGPKHAWYKWKYDPHLLDAVLMYAQRGHGKRSSYYSDYTFGLWAGDGQLLPIGKAYFGFTDEELKQLDTWIRNHKLQRFGPVQEVEKELVFEVAYDSAQTSARHKSGYALRFPRISRIRWDKPASEANQLEDLAKLV 456
            +EGLM+K  +++YV+G PK  WYKWK DP L+DAV+MYAQRGHGKRSS+YSDYTFG W  DG+LLPIGKAY GFTD ELK+LD W+R H + RFGPV+EV+KELVFEVA+DS   S RHKSG ALRFPRI RIRWDKPA EA+QLE L  ++
Sbjct:  168 IEGLMLKRKNTTYVAGRPKGQWYKWKRDPRLIDAVIMYAQRGHGKRSSFYSDYTFGCWQ-DGKLLPIGKAYSGFTDAELKRLDNWVRKHTIGRFGPVKEVKKELVFEVAFDSVHPSNRHKSGVALRFPRIHRIRWDKPADEADQLETLKMMI 318
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig106020.1295.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
R7QQL7_CHOCR3.420e-8580.52Putative ATP dependent DNA ligase n=1 Tax=Chondrus... [more]
UPI0003F83C721.690e-8481.58cisplatin damage response ATP-dependent DNA ligase... [more]
UPI0016775EE64.790e-8279.61cisplatin damage response ATP-dependent DNA ligase... [more]
UPI000378BA488.200e-8280.26cisplatin damage response ATP-dependent DNA ligase... [more]
A0A8J3G1E1_9PROT2.990e-8077.56DNA ligase (ATP) n=1 Tax=Algimonas arctica TaxID=1... [more]
A0A378LQJ9_9GAMM1.490e-7573.68DNA ligase (ATP) n=1 Tax=Legionella wadsworthii Ta... [more]
A0A378J159_9GAMM2.230e-7473.03DNA ligase (ATP) n=2 Tax=Legionella TaxID=445 RepI... [more]
A0A7G4RF43_9GAMM3.140e-7473.68DNA ligase (ATP) n=1 Tax=Legionella sp. PC997 TaxI... [more]
A0A4Q3JHD8_9HYPH7.150e-7471.43DNA ligase (ATP) (Fragment) n=1 Tax=Hyphomicrobial... [more]
UPI001BDF211E8.540e-7470.39cisplatin damage response ATP-dependent DNA ligase... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig106020contigM-pyrifera_M_contig106020:159..629 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score265.4
Seed ortholog evalue1.7e-68
Seed eggNOG ortholog1121948.AUAC01000003_gene2607
Preferred namelig
KEGG rclassRC00005
KEGG koko:K10747
KEGG ReactionR00381,R00382,R10822,R10823
KEGG Pathwayko03030,ko03410,ko03420,ko03430,map03030,map03410,map03420,map03430
Hectar predicted targeting categoryother localisation
GOsGO:0000287,GO:0003674,GO:0003824,GO:0003909,GO:0003910,GO:0005488,GO:0005575,GO:0005618,GO:0005622,GO:0005623,GO:0005737,GO:0006139,GO:0006259,GO:0006260,GO:0006261,GO:0006266,GO:0006271,GO:0006273,GO:0006281,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0016070,GO:0016874,GO:0016886,GO:0022616,GO:0030312,GO:0033554,GO:0034641,GO:0034645,GO:0043167,GO:0043169,GO:0043170,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044424,GO:0044464,GO:0046483,GO:0046872,GO:0050896,GO:0051103,GO:0051716,GO:0071704,GO:0071944,GO:0090304,GO:0140097,GO:1901360,GO:1901576
EggNOG free text desc.COG1793 ATP-dependent DNA ligase
EggNOG OGs1MV3S@1224,2TRQ9@28211,43X4I@69657,COG1793@1,COG1793@2
EC6.5.1.1,6.5.1.6,6.5.1.7
COG Functional cat.L
Best tax levelHyphomonadaceae
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03032,ko03400
Exons1
Model size471
Cds size471
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815569.7613432-CDS-M-pyrifera_M_contig106020:158..6291622815569.7613432-CDS-M-pyrifera_M_contig106020:158..629Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig106020 159..629 -
1692277468.3178096-CDS-M-pyrifera_M_contig106020:158..6291692277468.3178096-CDS-M-pyrifera_M_contig106020:158..629Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig106020 159..629 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig106020.1295.1prot_M-pyrifera_M_contig106020.1295.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig106020 159..629 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig106020.1295.1

>prot_M-pyrifera_M_contig106020.1295.1 ID=prot_M-pyrifera_M_contig106020.1295.1|Name=mRNA_M-pyrifera_M_contig106020.1295.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=157bp
MEGLMIKDLSSSYVSGGPKHAWYKWKYDPHLLDAVLMYAQRGHGKRSSYY
SDYTFGLWAGDGQLLPIGKAYFGFTDEELKQLDTWIRNHKLQRFGPVQEV
EKELVFEVAYDSAQTSARHKSGYALRFPRISRIRWDKPASEANQLEDLAK
LVDRAT*
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mRNA from alignment at M-pyrifera_M_contig106020:159..629-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig106020.1295.1 ID=mRNA_M-pyrifera_M_contig106020.1295.1|Name=mRNA_M-pyrifera_M_contig106020.1295.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=471bp|location=Sequence derived from alignment at M-pyrifera_M_contig106020:159..629- (Macrocystis pyrifera P11B4 male)
ATGGAAGGGCTGATGATCAAGGATCTGTCGAGCTCATATGTTTCCGGCGG
CCCGAAACATGCCTGGTACAAATGGAAATACGATCCCCACCTGCTCGACG
CGGTGCTGATGTATGCACAGCGCGGTCACGGAAAACGATCGTCCTACTAT
TCCGACTACACGTTCGGCCTTTGGGCCGGCGACGGTCAGCTTCTGCCAAT
CGGCAAGGCCTATTTCGGATTCACGGATGAAGAACTCAAACAGTTGGACA
CGTGGATACGGAATCACAAACTGCAGCGCTTCGGCCCGGTTCAGGAGGTC
GAGAAAGAATTGGTGTTCGAAGTCGCCTACGACAGCGCTCAGACCAGCGC
GCGCCATAAATCGGGCTATGCACTGCGCTTTCCGCGTATCAGCCGCATTC
GCTGGGACAAGCCGGCGTCCGAGGCCAATCAACTGGAGGATCTGGCCAAA
CTGGTCGACCGCGCCACATGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig106020:159..629-

>mRNA_M-pyrifera_M_contig106020.1295.1 ID=mRNA_M-pyrifera_M_contig106020.1295.1|Name=mRNA_M-pyrifera_M_contig106020.1295.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=942bp|location=Sequence derived from alignment at M-pyrifera_M_contig106020:159..629- (Macrocystis pyrifera P11B4 male)
ATGGAAGGGCTGATGATCAAGGATCTGTCGAGCTCATATGTTTCCGGCGG
CCCGAAACATGCCTGGTACAAATGGAAATACGATCCCCACCTGCTCGACG
CGGTGCTGATGTATGCACAGCGCGGTCACGGAAAACGATCGTCCTACTAT
TCCGACTACACGTTCGGCCTTTGGGCCGGCGACGGTCAGCTTCTGCCAAT
CGGCAAGGCCTATTTCGGATTCACGGATGAAGAACTCAAACAGTTGGACA
CGTGGATACGGAATCACAAACTGCAGCGCTTCGGCCCGGTTCAGGAGGTC
GAGAAAGAATTGGTGTTCGAAGTCGCCTACGACAGCGCTCAGACCAGCGC
GCGCCATAAATCGGGCTATGCACTGCGCTTTCCGCGTATCAGCCGCATTC
GCTGGGACAAGCCGGCGTCCGAGGCCAATCAACTGGAGGATCTGGCCAAA
CTGGTCGACCGCGCCACATGAATGGAAGGGCTGATGATCAAGGATCTGTC
GAGCTCATATGTTTCCGGCGGCCCGAAACATGCCTGGTACAAATGGAAAT
ACGATCCCCACCTGCTCGACGCGGTGCTGATGTATGCACAGCGCGGTCAC
GGAAAACGATCGTCCTACTATTCCGACTACACGTTCGGCCTTTGGGCCGG
CGACGGTCAGCTTCTGCCAATCGGCAAGGCCTATTTCGGATTCACGGATG
AAGAACTCAAACAGTTGGACACGTGGATACGGAATCACAAACTGCAGCGC
TTCGGCCCGGTTCAGGAGGTCGAGAAAGAATTGGTGTTCGAAGTCGCCTA
CGACAGCGCTCAGACCAGCGCGCGCCATAAATCGGGCTATGCACTGCGCT
TTCCGCGTATCAGCCGCATTCGCTGGGACAAGCCGGCGTCCGAGGCCAAT
CAACTGGAGGATCTGGCCAAACTGGTCGACCGCGCCACATGA
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