mRNA_M-pyrifera_M_contig100394.82.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig100394.82.1
Unique NamemRNA_M-pyrifera_M_contig100394.82.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
[Close]
BLAST of mRNA_M-pyrifera_M_contig100394.82.1 vs. uniprot
Match: A0A1Y1IKB4_KLENI (ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1IKB4_KLENI)

HSP 1 Score: 139 bits (351), Expect = 9.020e-38
Identity = 74/134 (55.22%), Postives = 91/134 (67.91%), Query Frame = 1 
Query:    4 TRTLGQCGKIAVVGGCLEYTGAPYYAAFTALKTGADLAHVFCAEEAGNAIKAYSPELIVHPVVPSERLDQPESLRAARNPSTA---FSAIRPWLSRVTSLVVGPGLGRSETMQHVAGEAIQTARDLSIPLVVDG 396
            T+  GQ GK+AV+GGC EYTGAP++AA +ALK GADL+HVFCA+ AG  IK+YSPELIVHPV+  E   + ES  A      A   FSAIR WL R   LVVGPGLGR + +     + I+ AR   +PLV+DG
Sbjct:   23 TKHKGQAGKVAVLGGCREYTGAPFFAAISALKIGADLSHVFCAKGAGTVIKSYSPELIVHPVL--EESYEHESASAQEREQVAEKAFSAIREWLPRFDCLVVGPGLGRDDLLLRTVAKVIREARSAELPLVLDG 154
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BLAST of mRNA_M-pyrifera_M_contig100394.82.1 vs. uniprot
Match: A0A2R6WRQ3_MARPO (ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=1 Tax=Marchantia polymorpha TaxID=3197 RepID=A0A2R6WRQ3_MARPO)

HSP 1 Score: 138 bits (348), Expect = 6.520e-36
Identity = 68/130 (52.31%), Postives = 84/130 (64.62%), Query Frame = 1 
Query:    7 RTLGQCGKIAVVGGCLEYTGAPYYAAFTALKTGADLAHVFCAEEAGNAIKAYSPELIVHPVVPSERLDQPESLRAARNPSTAFSAIRPWLSRVTSLVVGPGLGRSETMQHVAGEAIQTARDLSIPLVVDG 396
            R  GQ GKIAV+GGC EYTGAPYYAAF+AL+ GADL+HVFC E AG  IK+YSPELIVHPV+        E+L   +        +  WL R   LV+GPGLGR E +     E I  AR+ + P+++DG
Sbjct:  242 RHKGQAGKIAVIGGCREYTGAPYYAAFSALRMGADLSHVFCTEGAGTVIKSYSPELIVHPVLRESHDTNSENLSGRKLVEKVVGDVGKWLERFDCLVIGPGLGRDELLMECVSEIILMARENNTPMIIDG 371
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BLAST of mRNA_M-pyrifera_M_contig100394.82.1 vs. uniprot
Match: A0A176WLT7_MARPO (ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=1 Tax=Marchantia polymorpha subsp. ruderalis TaxID=1480154 RepID=A0A176WLT7_MARPO)

HSP 1 Score: 138 bits (348), Expect = 1.120e-35
Identity = 68/130 (52.31%), Postives = 84/130 (64.62%), Query Frame = 1 
Query:    7 RTLGQCGKIAVVGGCLEYTGAPYYAAFTALKTGADLAHVFCAEEAGNAIKAYSPELIVHPVVPSERLDQPESLRAARNPSTAFSAIRPWLSRVTSLVVGPGLGRSETMQHVAGEAIQTARDLSIPLVVDG 396
            R  GQ GKIAV+GGC EYTGAPYYAAF+AL+ GADL+HVFC E AG  IK+YSPELIVHPV+        E+L   +        +  WL R   LV+GPGLGR E +     E I  AR+ + P+++DG
Sbjct:  189 RHKGQAGKIAVIGGCREYTGAPYYAAFSALRMGADLSHVFCTEGAGTVIKSYSPELIVHPVLRESHDTNSENLSGRKLVEKVVGDVGKWLERFDCLVIGPGLGRDELLMECVSEIILMARENNTPMIIDG 318
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BLAST of mRNA_M-pyrifera_M_contig100394.82.1 vs. uniprot
Match: UPI000B797094 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase isoform X2 n=1 Tax=Chenopodium quinoa TaxID=63459 RepID=UPI000B797094)

HSP 1 Score: 133 bits (335), Expect = 1.590e-35
Identity = 69/132 (52.27%), Postives = 86/132 (65.15%), Query Frame = 1 
Query:    1 LTRTLGQCGKIAVVGGCLEYTGAPYYAAFTALKTGADLAHVFCAEEAGNAIKAYSPELIVHPVVPSERLDQPESLRAARNPSTAFSAIRPWLSRVTSLVVGPGLGRSETMQHVAGEAIQTARDLSIPLVVDG 396
            L+R  GQ GKIAVVGGC EYTGAPY+AA +ALK GADL+HVFC ++A   IK YSPELIVHPV+      + E      N     S ++ W+ R   LV+GPGLGR   +     E I+ AR L++PLV+DG
Sbjct:   70 LSRYKGQAGKIAVVGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPIIKCYSPELIVHPVLEESYNVKDEDKGIIAN--KVLSEVKKWMERFDCLVIGPGLGRDPFLLDCVSEIIKHARQLNVPLVIDG 199
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BLAST of mRNA_M-pyrifera_M_contig100394.82.1 vs. uniprot
Match: A0A2C9M341_BIOGL (ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=1 Tax=Biomphalaria glabrata TaxID=6526 RepID=A0A2C9M341_BIOGL)

HSP 1 Score: 133 bits (335), Expect = 2.320e-35
Identity = 68/126 (53.97%), Postives = 82/126 (65.08%), Query Frame = 1 
Query:   16 GQCGKIAVVGGCLEYTGAPYYAAFTALKTGADLAHVFCAEEAGNAIKAYSPELIVHPVVPSERLDQPESLRAARNPSTAFSAIRPWLSRVTSLVVGPGLGRSETMQHVAGEAIQTARDLSIPLVVD 393
            GQ G++AV+GGC EYTGAPY+AA TALK GADL+HVFC   A   IK+YSPELIVHPV+ S                TA + I  WL R  SLVVGPGLGR E +  +  E IQ AR+  +P+V+D
Sbjct:   59 GQAGRVAVLGGCQEYTGAPYFAAITALKVGADLSHVFCTSSAATVIKSYSPELIVHPVLDS---------------LTAVTEISQWLPRFHSLVVGPGLGRDEQLLEIVSEVIQKAREQQLPIVID 169
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BLAST of mRNA_M-pyrifera_M_contig100394.82.1 vs. uniprot
Match: A0A7S0PSL0_MICPS (Hypothetical protein (Fragment) n=1 Tax=Micromonas pusilla TaxID=38833 RepID=A0A7S0PSL0_MICPS)

HSP 1 Score: 132 bits (332), Expect = 3.220e-35
Identity = 73/135 (54.07%), Postives = 88/135 (65.19%), Query Frame = 1 
Query:   16 GQCGKIAVVGGCLEYTGAPYYAAFTALKTGADLAHVFCAEEAGNAIKAYSPELIVHPVVPSERLD-----QPESLRAARNPSTA-FSA---IRPWLSRVTSLVVGPGLGRSETMQHVAGEAIQTARDLSIPLVVD 393
            GQ GKI VVGGC EYTGAPY+AA +AL+ GADLAHVFCAE AG  IK+YSPELIVHP +   R +       E++ A      A F A   + PWL R+ +LVVGPGLGR  TM  +A   I  A +  +PLV+D
Sbjct:   43 GQAGKIGVVGGCSEYTGAPYFAAMSALRMGADLAHVFCAEGAGQVIKSYSPELIVHPYLREGRKEVTVTVDGEAVHAVTYDEEAVFEAMERVTPWLHRMDALVVGPGLGRDPTMSEIAKRIIAFAHERDVPLVID 177
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BLAST of mRNA_M-pyrifera_M_contig100394.82.1 vs. uniprot
Match: A0A7C9E1M2_OPUST (ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=3 Tax=Opuntia streptacantha TaxID=393608 RepID=A0A7C9E1M2_OPUST)

HSP 1 Score: 133 bits (335), Expect = 3.570e-35
Identity = 66/132 (50.00%), Postives = 87/132 (65.91%), Query Frame = 1 
Query:    1 LTRTLGQCGKIAVVGGCLEYTGAPYYAAFTALKTGADLAHVFCAEEAGNAIKAYSPELIVHPVVPSERLDQPESLRAARNPSTAFSAIRPWLSRVTSLVVGPGLGRSETMQHVAGEAIQTARDLSIPLVVDG 396
            L+R  GQ GKIAV+GGC EYTGAPY+AA +ALK GADL+HVFC ++A   IK+YSPELIVHP++        E  R+  N     + +  W+ R   +VVGPGLGR   +     E ++ AR L+IPL++DG
Sbjct:   64 LSRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPIIKSYSPELIVHPILEESYSVSDEDKRSTAN--KVLAEVNKWMERFDCIVVGPGLGRDPFLLDCVSEIMKHARQLNIPLIIDG 193
[Close]
BLAST of mRNA_M-pyrifera_M_contig100394.82.1 vs. uniprot
Match: A0A453HES1_AEGTS (Uncharacterized protein n=3 Tax=Aegilops tauschii subsp. strangulata TaxID=200361 RepID=A0A453HES1_AEGTS)

HSP 1 Score: 129 bits (323), Expect = 8.010e-35
Identity = 66/133 (49.62%), Postives = 88/133 (66.17%), Query Frame = 1 
Query:    7 RTLGQCGKIAVVGGCLEYTGAPYYAAFTALKTGADLAHVFCAEEAGNAIKAYSPELIVHPVVP---SERLDQPESLRAARNPSTAFSAIRPWLSRVTSLVVGPGLGRSETMQHVAGEAIQTARDLSIPLVVDG 396
            R  GQ GKIA++GGC EYTGAPY+AA +AL+ GADL+HVFC ++A   IK+YSPELIVHP++    S R D+ ES+      S+  + +  W+ R   +VVGPGLGR   +    G  ++ AR  +IP VVDG
Sbjct:   90 RHKGQAGKIAIIGGCREYTGAPYFAAISALRVGADLSHVFCTKDAATVIKSYSPELIVHPILEESYSVRDDERESVS-----SSILAEVIKWMERFDCIVVGPGLGRDSFLMDCVGNIMRHARQANIPTVVDG 217
[Close]
BLAST of mRNA_M-pyrifera_M_contig100394.82.1 vs. uniprot
Match: A0A0K9RRK5_SPIOL (ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=2 Tax=Spinacia oleracea TaxID=3562 RepID=A0A0K9RRK5_SPIOL)

HSP 1 Score: 132 bits (332), Expect = 8.820e-35
Identity = 69/132 (52.27%), Postives = 86/132 (65.15%), Query Frame = 1 
Query:    1 LTRTLGQCGKIAVVGGCLEYTGAPYYAAFTALKTGADLAHVFCAEEAGNAIKAYSPELIVHPVVPSERLDQPESLRAARNPSTAFSAIRPWLSRVTSLVVGPGLGRSETMQHVAGEAIQTARDLSIPLVVDG 396
            L+R  GQ GKIAVVGGC EYTGAPY+AA +ALK GADL+HVFC ++A   IK+YSPELIVHPV+      + E      N     S +  W+ R   LV+GPGLGR   +     E ++ AR L+IPLV+DG
Sbjct:   58 LSRHKGQAGKIAVVGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPIIKSYSPELIVHPVLEESYNVKDEDKGFIAN--KVLSEVNKWMERFDCLVIGPGLGRDPFLLDCVSEILKHARQLNIPLVIDG 187
[Close]
BLAST of mRNA_M-pyrifera_M_contig100394.82.1 vs. uniprot
Match: A0A1Q3AYV5_CEPFO (ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=1 Tax=Cephalotus follicularis TaxID=3775 RepID=A0A1Q3AYV5_CEPFO)

HSP 1 Score: 132 bits (331), Expect = 1.340e-34
Identity = 70/136 (51.47%), Postives = 87/136 (63.97%), Query Frame = 1 
Query:    1 LTRTLGQCGKIAVVGGCLEYTGAPYYAAFTALKTGADLAHVFCAEEAGNAIKAYSPELIVHPVVPSER----LDQPESLRAARNPSTAFSAIRPWLSRVTSLVVGPGLGRSETMQHVAGEAIQTARDLSIPLVVDG 396
            L+R  GQ GKIAVVGGC EYTGAPY++A +ALK GADL+HVFC ++A   IK YSPELIVHP++        L   +    +RN   A S +  W+ R   LVVGPGLGR   +     E I+ AR L +PLV+DG
Sbjct:   60 LSRHKGQAGKIAVVGGCREYTGAPYFSAISALKIGADLSHVFCTKDAAPVIKGYSPELIVHPILMESYSVSGLSDEDRRYISRN---AISEVDKWMERFDCLVVGPGLGRDPLLLECVSEIIKHARQLKVPLVIDG 192
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig100394.82.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1Y1IKB4_KLENI9.020e-3855.22ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=1 ... [more]
A0A2R6WRQ3_MARPO6.520e-3652.31ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=1 ... [more]
A0A176WLT7_MARPO1.120e-3552.31ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=1 ... [more]
UPI000B7970941.590e-3552.27ATP-dependent (S)-NAD(P)H-hydrate dehydratase isof... [more]
A0A2C9M341_BIOGL2.320e-3553.97ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=1 ... [more]
A0A7S0PSL0_MICPS3.220e-3554.07Hypothetical protein (Fragment) n=1 Tax=Micromonas... [more]
A0A7C9E1M2_OPUST3.570e-3550.00ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=3 ... [more]
A0A453HES1_AEGTS8.010e-3549.62Uncharacterized protein n=3 Tax=Aegilops tauschii ... [more]
A0A0K9RRK5_SPIOL8.820e-3552.27ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=2 ... [more]
A0A1Q3AYV5_CEPFO1.340e-3451.47ATP-dependent (S)-NAD(P)H-hydrate dehydratase n=1 ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig100394contigM-pyrifera_M_contig100394:41..436 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score134.0
Seed ortholog evalue5e-29
Seed eggNOG ortholog161934.XP_010672214.1
KEGG koko:K17757
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005829,GO:0006139,GO:0006725,GO:0006732,GO:0006733,GO:0006734,GO:0006739,GO:0006753,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0009117,GO:0009507,GO:0009536,GO:0009987,GO:0016829,GO:0016835,GO:0016836,GO:0016853,GO:0016854,GO:0019362,GO:0019637,GO:0019674,GO:0034641,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044238,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0046496,GO:0047453,GO:0051186,GO:0052856,GO:0052857,GO:0055086,GO:0071704,GO:0072524,GO:1901360,GO:1901564
EggNOG free text desc.Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration
EggNOG OGs37IRG@33090,3GCV7@35493,COG0063@1,KOG3974@2759
EC4.2.1.93
COG Functional cat.G
Best tax levelStreptophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000
Exons1
Model size396
Cds size396
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815499.9482203-CDS-M-pyrifera_M_contig100394:40..4361622815499.9482203-CDS-M-pyrifera_M_contig100394:40..436Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100394 41..436 -
1692277439.368108-CDS-M-pyrifera_M_contig100394:40..4361692277439.368108-CDS-M-pyrifera_M_contig100394:40..436Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100394 41..436 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig100394.82.1prot_M-pyrifera_M_contig100394.82.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig100394 41..436 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig100394.82.1

>prot_M-pyrifera_M_contig100394.82.1 ID=prot_M-pyrifera_M_contig100394.82.1|Name=mRNA_M-pyrifera_M_contig100394.82.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=132bp
LTRTLGQCGKIAVVGGCLEYTGAPYYAAFTALKTGADLAHVFCAEEAGNA
IKAYSPELIVHPVVPSERLDQPESLRAARNPSTAFSAIRPWLSRVTSLVV
GPGLGRSETMQHVAGEAIQTARDLSIPLVVDG
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mRNA from alignment at M-pyrifera_M_contig100394:41..436-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig100394.82.1 ID=mRNA_M-pyrifera_M_contig100394.82.1|Name=mRNA_M-pyrifera_M_contig100394.82.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=396bp|location=Sequence derived from alignment at M-pyrifera_M_contig100394:41..436- (Macrocystis pyrifera P11B4 male)
CTGACAAGAACACTAGGGCAATGCGGAAAGATCGCTGTAGTGGGCGGGTG
CCTGGAGTATACCGGCGCCCCTTACTACGCAGCCTTCACAGCACTCAAAA
CGGGTGCTGACCTCGCCCACGTCTTTTGTGCTGAAGAGGCGGGCAACGCA
ATCAAGGCCTACAGCCCGGAGCTCATCGTTCACCCTGTCGTGCCCTCCGA
GAGGCTTGATCAGCCTGAGTCTCTCCGTGCTGCGAGGAACCCCTCCACTG
CCTTCAGTGCTATTCGCCCCTGGCTCTCTCGGGTGACATCGCTCGTCGTT
GGCCCTGGCCTGGGCAGGAGCGAAACCATGCAGCACGTCGCAGGAGAGGC
CATCCAGACTGCTCGAGACCTGAGCATTCCCCTCGTCGTAGACGGC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig100394:41..436-

>mRNA_M-pyrifera_M_contig100394.82.1 ID=mRNA_M-pyrifera_M_contig100394.82.1|Name=mRNA_M-pyrifera_M_contig100394.82.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=792bp|location=Sequence derived from alignment at M-pyrifera_M_contig100394:41..436- (Macrocystis pyrifera P11B4 male)
CTGACAAGAACACTAGGGCAATGCGGAAAGATCGCTGTAGTGGGCGGGTG
CCTGGAGTATACCGGCGCCCCTTACTACGCAGCCTTCACAGCACTCAAAA
CGGGTGCTGACCTCGCCCACGTCTTTTGTGCTGAAGAGGCGGGCAACGCA
ATCAAGGCCTACAGCCCGGAGCTCATCGTTCACCCTGTCGTGCCCTCCGA
GAGGCTTGATCAGCCTGAGTCTCTCCGTGCTGCGAGGAACCCCTCCACTG
CCTTCAGTGCTATTCGCCCCTGGCTCTCTCGGGTGACATCGCTCGTCGTT
GGCCCTGGCCTGGGCAGGAGCGAAACCATGCAGCACGTCGCAGGAGAGGC
CATCCAGACTGCTCGAGACCTGAGCATTCCCCTCGTCGTAGACGGCCTGA
CAAGAACACTAGGGCAATGCGGAAAGATCGCTGTAGTGGGCGGGTGCCTG
GAGTATACCGGCGCCCCTTACTACGCAGCCTTCACAGCACTCAAAACGGG
TGCTGACCTCGCCCACGTCTTTTGTGCTGAAGAGGCGGGCAACGCAATCA
AGGCCTACAGCCCGGAGCTCATCGTTCACCCTGTCGTGCCCTCCGAGAGG
CTTGATCAGCCTGAGTCTCTCCGTGCTGCGAGGAACCCCTCCACTGCCTT
CAGTGCTATTCGCCCCTGGCTCTCTCGGGTGACATCGCTCGTCGTTGGCC
CTGGCCTGGGCAGGAGCGAAACCATGCAGCACGTCGCAGGAGAGGCCATC
CAGACTGCTCGAGACCTGAGCATTCCCCTCGTCGTAGACGGC
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