mRNA_M-pyrifera_M_contig103635.776.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig103635.776.1
Unique NamemRNA_M-pyrifera_M_contig103635.776.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig103635.776.1 vs. uniprot
Match: W7U2X6_9STRA (U2 snrnp-associated splicing factor putative n=4 Tax=Monodopsidaceae TaxID=425072 RepID=W7U2X6_9STRA)

HSP 1 Score: 63.9 bits (154), Expect = 1.540e-8
Identity = 25/44 (56.82%), Postives = 31/44 (70.45%), Query Frame = 1
Query:  430 RFLWPAATPEHRYYRWRVLSFVHGDTERKWRTAPFRVAVSGPVW 561
            RFLW   +PE  +YRWRV SF+ GDTE +WR  PF++ V GP W
Sbjct:  504 RFLWEGDSPEALFYRWRVFSFLMGDTETRWRVRPFQMTVDGPFW 547          
BLAST of mRNA_M-pyrifera_M_contig103635.776.1 vs. uniprot
Match: A0A7S3RUD6_9SPIT (Hypothetical protein (Fragment) n=1 Tax=Strombidinopsis acuminata TaxID=141414 RepID=A0A7S3RUD6_9SPIT)

HSP 1 Score: 63.5 bits (153), Expect = 1.570e-8
Identity = 51/200 (25.50%), Postives = 80/200 (40.00%), Query Frame = 1
Query:    1 DEERAKGTTRGYVAFATREAAVAALSDMQGVLIGGNPLKVTWGRPLRRLASKTSTXXXXXXXXXXXXXXXXXXXXXHSGILPPPASLSSDASGNGXXXXASVGGATGGGMKAKSGSWPAEEVREGDVV-------------FEVEWPSDARRRRVSRFLWPAATPEHRYYRWRVLSFVHGDTERKWRTAPFRVAVSGPVW 561
            +EER +    G+VAF  R  A  A  +M    + G  +++ WG+ + R  ++  T                      SG + PPA+ SS    +     A V           +   P   + + +++             FE    +        RFL+ A+TPEHRYYRW+V+S    DT   W T  F++   GP+W
Sbjct:  159 EEERMRQRLNGFVAFVKRADAAQAKDEMNETELNGFVMRIGWGKAVAR-PTQAITLASLQGRSASASGSAMASTGPPSGHVSPPAAFSSTTPSSNSAASAVVKAQPPRPSGPTTMVLPPTNLGQRELIDRLALFVAEEGHAFEQAIMAREESNSQYRFLFDASTPEHRYYRWKVVSLCACDTMDAWDTGSFQLEAGGPIW 357          
BLAST of mRNA_M-pyrifera_M_contig103635.776.1 vs. uniprot
Match: A0A834IAD5_RHYFE (Uncharacterized protein n=1 Tax=Rhynchophorus ferrugineus TaxID=354439 RepID=A0A834IAD5_RHYFE)

HSP 1 Score: 58.2 bits (139), Expect = 1.430e-6
Identity = 54/205 (26.34%), Postives = 83/205 (40.49%), Query Frame = 1
Query:    1 DEERAKGTTRGYVAFATREAAVAALSDMQGVLIGGNPLKVTWGR----PLRRLASKTSTXXXXXXXXXXXXXXXXXXXXXHSGILPPPASLSSDASGNGXXXXASVGGATGGGMKAKSGSWPAEE-------------VREGDVVFEVEWPSDARRRRVSRFLWPAATPEHRYYRWRVLSFVHGDTERKWRTAPFRVAVSGPVWK 564
            DEE+A+G   G+VA+ +R+    AL ++ G  I G  +K+ WG+    P   +                              +LP      SD   N     A+V               P+++             VREG + FE    +      + RFL+   +P H YYRW+V S +HGD++++W T  FR+   G VWK
Sbjct:  223 DEEKARGRNCGFVAYMSRKDGERALRNLNGKEIVGYEMKMGWGKSIIIPPHPIYIPPVLLDVSQPPPPSGLPFNAQPAVEDKDVLP-----KSDEELNAILKRATV-----------KVVIPSDKNLLVLIHRMVEFVVREGPM-FEAMIMNRELHNPLFRFLFENHSPAHIYYRWKVYSILHGDSQKEWSTKEFRMFRDGSVWK 410          
BLAST of mRNA_M-pyrifera_M_contig103635.776.1 vs. uniprot
Match: A0A8J9UBM1_BRAAE (Hypothetical protein n=1 Tax=Brassicogethes aeneus TaxID=1431903 RepID=A0A8J9UBM1_BRAAE)

HSP 1 Score: 58.2 bits (139), Expect = 1.430e-6
Identity = 51/192 (26.56%), Postives = 79/192 (41.15%), Query Frame = 1
Query:    1 DEERAKGTTRGYVAFATREAAVAALSDMQGVLIGGNPLKVTWGR----PLRRLASKTSTXXXXXXXXXXXXXXXXXXXXXHSGILPPPASLSSDASGNGXXXXASVGGATGGGMKAKSGSWPAEEVREGDVVFEVEWPSDARRRRVSRFLWPAATPEHRYYRWRVLSFVHGDTERKWRTAPFRVAVSGPVWK 564
            DEE+A+G   G+VA+ +R+    AL ++ G  I G  +K+ WG+    P   +                              ILP  A   +D           V   T   +           VREG + FE    +   +  + RFL+   +P H YYRW++ S  HGD++++W T  FR+   G +WK
Sbjct:  230 DEEKARGRNCGFVAYMSRKDGERALRNLNGKDIVGYEMKLGWGKSVIIPPHPIYIPPCLLEVSMPPPPSGLPFNAQPAPNDVDILPKSAEELTDLL---TRATVKVVIPTDRNLLMLIHRMVEFVVREGPM-FEAMIMNREIQNHMYRFLFENQSPAHTYYRWKLYSITHGDSQKEWSTKEFRMFKDGSIWK 417          
BLAST of mRNA_M-pyrifera_M_contig103635.776.1 vs. uniprot
Match: V5IAX6_ANOGL (U2 snRNP-associated SURP motif-containing protein (Fragment) n=2 Tax=Anoplophora glabripennis TaxID=217634 RepID=V5IAX6_ANOGL)

HSP 1 Score: 57.8 bits (138), Expect = 1.640e-6
Identity = 27/67 (40.30%), Postives = 40/67 (59.70%), Query Frame = 1
Query:  364 VREGDVVFEVEWPSDARRRRVSRFLWPAATPEHRYYRWRVLSFVHGDTERKWRTAPFRVAVSGPVWK 564
            VREG + FE    +        RFL+   +P H YYRW+V S +HGD++++W+T  FR+   G +WK
Sbjct:  345 VREGPM-FEAMIMNREINNAQFRFLFENQSPTHIYYRWKVFSILHGDSQKEWKTKEFRMFKDGSIWK 410          
BLAST of mRNA_M-pyrifera_M_contig103635.776.1 vs. uniprot
Match: UPI000C20B860 (U2 snRNP-associated SURP motif-containing protein isoform X1 n=2 Tax=Onthophagus taurus TaxID=166361 RepID=UPI000C20B860)

HSP 1 Score: 57.8 bits (138), Expect = 1.930e-6
Identity = 48/192 (25.00%), Postives = 78/192 (40.62%), Query Frame = 1
Query:    1 DEERAKGTTRGYVAFATREAAVAALSDMQGVLIGGNPLKVTWGR----PLRRLASKTSTXXXXXXXXXXXXXXXXXXXXXHSGILPPPASLSSDASGNGXXXXASVGGATGGGMKAKSGSWPAEEVREGDVVFEVEWPSDARRRRVSRFLWPAATPEHRYYRWRVLSFVHGDTERKWRTAPFRVAVSGPVWK 564
            DEE+++G   G+VA+ +R+    AL ++ G  I G  +K+ WG+    P   +    S                         +LP      ++  G            T   +           VREG + FE    +      V RFL+   +P H YYRW++ S +HGD++++W    FR+   G +WK
Sbjct:  222 DEEKSRGRNCGFVAYMSRKDGERALRNLNGKDIIGYEMKLGWGKSVVIPPHPIYIPPSLLELSLPPPPSGLPFNAQPQGKDKDVLPKSTEEFNEVLGRAVVKVVIP---TDRNLLMLIHRMVEFVVREGPM-FEAMIMNREINNPVFRFLFENQSPAHLYYRWKIYSIMHGDSQKEWSHKEFRMFTEGSIWK 409          
BLAST of mRNA_M-pyrifera_M_contig103635.776.1 vs. uniprot
Match: A0A8K0D4U6_9COLE (Uncharacterized protein n=1 Tax=Ignelater luminosus TaxID=2038154 RepID=A0A8K0D4U6_9COLE)

HSP 1 Score: 57.4 bits (137), Expect = 2.570e-6
Identity = 48/192 (25.00%), Postives = 79/192 (41.15%), Query Frame = 1
Query:    1 DEERAKGTTRGYVAFATREAAVAALSDMQGVLIGGNPLKVTWGR----PLRRLASKTSTXXXXXXXXXXXXXXXXXXXXXHSGILPPPASLSSDASGNGXXXXASVGGATGGGMKAKSGSWPAEEVREGDVVFEVEWPSDARRRRVSRFLWPAATPEHRYYRWRVLSFVHGDTERKWRTAPFRVAVSGPVWK 564
            DEE+A+G   G+VA+ +R+    AL ++ G  + G  +K+ WG+    P   +    +                         +LP  A   +D               T   +           +REG + FE    +      + RFL+   +P H YYRW++ S +HGD++++W T  FR+  SG  WK
Sbjct:  114 DEEKARGRNCGFVAYMSRKDGERALRNLNGKDVLGYEMKLGWGKSVIIPPHPIYIPPALLELSLPPPPSGLPFNAQPVPKDRDVLPTTAEELNDILSRAVVKVVIP---TDRNLLMLIHRMVEFVIREGPM-FEAMIMNREINNSLFRFLFENQSPAHIYYRWKLYSIMHGDSQKEWSTKEFRMFKSGSAWK 301          
BLAST of mRNA_M-pyrifera_M_contig103635.776.1 vs. uniprot
Match: A0A6P7FQL7_DIAVI (U2 snRNP-associated SURP motif-containing protein n=1 Tax=Diabrotica virgifera virgifera TaxID=50390 RepID=A0A6P7FQL7_DIAVI)

HSP 1 Score: 57.4 bits (137), Expect = 2.610e-6
Identity = 27/67 (40.30%), Postives = 41/67 (61.19%), Query Frame = 1
Query:  364 VREGDVVFEVEWPSDARRRRVSRFLWPAATPEHRYYRWRVLSFVHGDTERKWRTAPFRVAVSGPVWK 564
            VREG + FE    +   +    RFL+   +P H YYRW+V S +HGD++++W T  FR+  +G +WK
Sbjct:  345 VREGPM-FEAMIMNKEIQNPQFRFLFENQSPSHIYYRWKVYSILHGDSQKEWCTKEFRMFTNGSIWK 410          
BLAST of mRNA_M-pyrifera_M_contig103635.776.1 vs. uniprot
Match: A0A1W4X8J8_AGRPL (U2 snRNP-associated SURP motif-containing protein n=1 Tax=Agrilus planipennis TaxID=224129 RepID=A0A1W4X8J8_AGRPL)

HSP 1 Score: 55.5 bits (132), Expect = 1.190e-5
Identity = 53/216 (24.54%), Postives = 82/216 (37.96%), Query Frame = 1
Query:    1 DEERAKGTTRGYVAFATREAAVAALSDMQGVLIGGNPLKVTWGRPLRRLASKTSTXXXXXXXXXXXXXXXXXXXXXHSGILPPPASLSSDASG---------------NGXXXXASVGGATGGGMKAKSGSWPAEE-------------VREGDVVFEVEWPSDARRRRVSRFLWPAATPEHRYYRWRVLSFVHGDTERKWRTAPFRVAVSGPVWK 564
            +EE+AKG+  G+VA+  R+ A  AL ++    I G+ +K+ WG+P+                                 + PPP+ L  +A                 N     A V               P +              VREG + FE    +      + RFL+   +P H YYRW+V S  HG+++R+W    FR+   G +WK
Sbjct:  217 EEEKAKGSNCGFVAYMARKDAERALRNLNEKEISGSDIKLGWGKPV----------------ILPPFPIYIPPPLMELSMPPPPSGLPFNAQPIAQDRDILPKTQDELNQILYRAVV-----------KVVMPKDRALLILINRMVEFVVREGPM-FEAMIMNREINNPMFRFLFENQSPSHIYYRWKVFSITHGESQREWSNKEFRMFKEGSIWK 404          
BLAST of mRNA_M-pyrifera_M_contig103635.776.1 vs. uniprot
Match: UPI00083C6A79 (U2 snRNP-associated SURP motif-containing protein isoform X1 n=6 Tax=Nicrophorus vespilloides TaxID=110193 RepID=UPI00083C6A79)

HSP 1 Score: 55.5 bits (132), Expect = 1.190e-5
Identity = 26/67 (38.81%), Postives = 39/67 (58.21%), Query Frame = 1
Query:  364 VREGDVVFEVEWPSDARRRRVSRFLWPAATPEHRYYRWRVLSFVHGDTERKWRTAPFRVAVSGPVWK 564
            VREG + FE    +      + RFL+   +P H YYRW++ S +HGD++++W T  FR+   G  WK
Sbjct:  369 VREGPM-FEAMIMNRELHNPLFRFLFENQSPAHIYYRWKIFSIMHGDSQKEWTTREFRMFKDGSPWK 434          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig103635.776.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 22
Match NameE-valueIdentityDescription
W7U2X6_9STRA1.540e-856.82U2 snrnp-associated splicing factor putative n=4 T... [more]
A0A7S3RUD6_9SPIT1.570e-825.50Hypothetical protein (Fragment) n=1 Tax=Strombidin... [more]
A0A834IAD5_RHYFE1.430e-626.34Uncharacterized protein n=1 Tax=Rhynchophorus ferr... [more]
A0A8J9UBM1_BRAAE1.430e-626.56Hypothetical protein n=1 Tax=Brassicogethes aeneus... [more]
V5IAX6_ANOGL1.640e-640.30U2 snRNP-associated SURP motif-containing protein ... [more]
UPI000C20B8601.930e-625.00U2 snRNP-associated SURP motif-containing protein ... [more]
A0A8K0D4U6_9COLE2.570e-625.00Uncharacterized protein n=1 Tax=Ignelater luminosu... [more]
A0A6P7FQL7_DIAVI2.610e-640.30U2 snRNP-associated SURP motif-containing protein ... [more]
A0A1W4X8J8_AGRPL1.190e-524.54U2 snRNP-associated SURP motif-containing protein ... [more]
UPI00083C6A791.190e-538.81U2 snRNP-associated SURP motif-containing protein ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig103635contigM-pyrifera_M_contig103635:1..648 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score63.9
Seed ortholog evalue9e-08
Seed eggNOG ortholog130081.XP_005705132.1
Preferred nameU2SURP
KEGG koko:K02878,ko:K12842
KEGG Pathwayko03010,ko03040,map03010,map03040
KEGG ModuleM00178
Hectar predicted targeting categoryother localisation
GOsGO:0000375,GO:0000377,GO:0000398,GO:0003674,GO:0003676,GO:0003723,GO:0003729,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005681,GO:0005685,GO:0005686,GO:0006139,GO:0006396,GO:0006397,GO:0006725,GO:0006807,GO:0008150,GO:0008152,GO:0008380,GO:0009987,GO:0010467,GO:0016070,GO:0016071,GO:0017069,GO:0030532,GO:0030619,GO:0031974,GO:0031981,GO:0032991,GO:0034641,GO:0035613,GO:0035614,GO:0042802,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0045292,GO:0046483,GO:0070013,GO:0071704,GO:0090304,GO:0097159,GO:0097525,GO:0120114,GO:1901360,GO:1901363,GO:1990904
EggNOG free text desc.U2 snRNP-associated SURP
EggNOG OGsKOG0151@1,KOG0151@2759
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01610,ko00000,ko00001,ko00002,ko03011,ko03041
Exons2
Model size564
Cds size564
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815542.524032-CDS-M-pyrifera_M_contig103635:0..1371622815542.524032-CDS-M-pyrifera_M_contig103635:0..137Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103635 1..137 -
1692277456.3253334-CDS-M-pyrifera_M_contig103635:0..1371692277456.3253334-CDS-M-pyrifera_M_contig103635:0..137Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103635 1..137 -
1622815542.5352204-CDS-M-pyrifera_M_contig103635:221..6481622815542.5352204-CDS-M-pyrifera_M_contig103635:221..648Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103635 222..648 -
1692277456.3422759-CDS-M-pyrifera_M_contig103635:221..6481692277456.3422759-CDS-M-pyrifera_M_contig103635:221..648Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig103635 222..648 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig103635.776.1prot_M-pyrifera_M_contig103635.776.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig103635 1..648 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig103635.776.1

>prot_M-pyrifera_M_contig103635.776.1 ID=prot_M-pyrifera_M_contig103635.776.1|Name=mRNA_M-pyrifera_M_contig103635.776.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=188bp
DEERAKGTTRGYVAFATREAAVAALSDMQGVLIGGNPLKVTWGRPLRRLA
SKTSTSSSPSSSSSSSSSSSSSSSPSHSGILPPPASLSSDASGNGKGVGA
SVGGATGGGMKAKSGSWPAEEVREGDVVFEVEWPSDARRRRVSRFLWPAA
TPEHRYYRWRVLSFVHGDTERKWRTAPFRVAVSGPVWK
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mRNA from alignment at M-pyrifera_M_contig103635:1..648-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig103635.776.1 ID=mRNA_M-pyrifera_M_contig103635.776.1|Name=mRNA_M-pyrifera_M_contig103635.776.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=648bp|location=Sequence derived from alignment at M-pyrifera_M_contig103635:1..648- (Macrocystis pyrifera P11B4 male)
GACGAAGAGCGTGCGAAAGGGACAACAAGAGGTTATGTCGCATTTGCAAC ACGTGAAGCAGCAGTAGCAGCCTTATCTGACATGCAAGGAGTGTTGATTG GTGGAAATCCACTGAAGGTGACATGGGGACGTCCATTACGCCGTCTCGCT TCAAAAACATCCACTTCCTCCTCCCCCTCTTCCTCTTCCTCTTCCTCATC CTCTTCCTCATCCTCCTCTTCCCCATCACATTCTGGTATTCTTCCTCCTC CTGCCTCTTTGAGTTCTGATGCATCAGGAAATGGGAAAGGTGTAGGAGCG AGTGTTGGAGGTGCTACAGGAGGAGGTATGAAGGCAAAATCTGGATCATG GCCTGCAGAGGAGGTTCGAGAAGGAGATGTTGTGTTTGAAGTGGAGTGGC CATCTGATGCAAGGAGACGGCGAGTTTGTGATCGAGTTGCTGCTGCTGTA GCAGAAGGTGGATATGAAGTTGAACGAGCATTGATGTAGCATCCATGGGC TGCGCGAGAAGCACGGTTTTTATGGCCTGCTGCTACACCAGAGCATAGGT ATTACAGATGGCGAGTGTTGAGTTTTGTTCATGGGGATACAGAACGGAAG TGGAGAACTGCACCATTTCGAGTTGCTGTTTCTGGTCCCGTGTGGAAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig103635:1..648-

>mRNA_M-pyrifera_M_contig103635.776.1 ID=mRNA_M-pyrifera_M_contig103635.776.1|Name=mRNA_M-pyrifera_M_contig103635.776.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1128bp|location=Sequence derived from alignment at M-pyrifera_M_contig103635:1..648- (Macrocystis pyrifera P11B4 male)
GACGAAGAGCGTGCGAAAGGGACAACAAGAGGTTATGTCGCATTTGCAAC
ACGTGAAGCAGCAGTAGCAGCCTTATCTGACATGCAAGGAGTGTTGATTG
GTGGAAATCCACTGAAGGTGACATGGGGACGTCCATTACGCCGTCTCGCT
TCAAAAACATCCACTTCCTCCTCCCCCTCTTCCTCTTCCTCTTCCTCATC
CTCTTCCTCATCCTCCTCTTCCCCATCACATTCTGGTATTCTTCCTCCTC
CTGCCTCTTTGAGTTCTGATGCATCAGGAAATGGGAAAGGTGTAGGAGCG
AGTGTTGGAGGTGCTACAGGAGGAGGTATGAAGGCAAAATCTGGATCATG
GCCTGCAGAGGAGGTTCGAGAAGGAGATGTTGTGTTTGAAGTGGAGTGGC
CATCTGATGCAAGGAGACGGCGAGTTTGACGAAGAGCGTGCGAAAGGGAC
AACAAGAGGTTATGTCGCATTTGCAACACGTGAAGCAGCAGTAGCAGCCT
TATCTGACATGCAAGGAGTGTTGATTGGTGGAAATCCACTGAAGGTGACA
TGGGGACGTCCATTACGCCGTCTCGCTTCAAAAACATCCACTTCCTCCTC
CCCCTCTTCCTCTTCCTCTTCCTCATCCTCTTCCTCATCCTCCTCTTCCC
CATCACATTCTGGTATTCTTCCTCCTCCTGCCTCTTTGAGTTCTGATGCA
TCAGGAAATGGGAAAGGTGTAGGAGCGAGTGTTGGAGGTGCTACAGGAGG
AGGTATGAAGGCAAAATCTGGATCATGGCCTGCAGAGGAGGTTCGAGAAG
GAGATGTTGTGTTTGAAGTGGAGTGGCCATCTGATGCAAGGAGACGGCGA
GTTTCACGGTTTTTATGGCCTGCTGCTACACCAGAGCATAGGTATTACAG
ATGGCGAGTGTTGAGTTTTGTTCATGGGGATACAGAACGGAAGTGGAGAA
CTGCACCATTTCGAGTTGCTGTTTCTGGTCCCGTGTGGAAACACGGTTTT
TATGGCCTGCTGCTACACCAGAGCATAGGTATTACAGATGGCGAGTGTTG
AGTTTTGTTCATGGGGATACAGAACGGAAGTGGAGAACTGCACCATTTCG
AGTTGCTGTTTCTGGTCCCGTGTGGAAA
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