mRNA_M-pyrifera_M_contig102834.607.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig102834.607.1
Unique NamemRNA_M-pyrifera_M_contig102834.607.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig102834.607.1 vs. uniprot
Match: A0A2E7K5C2_9PLAN (tRNA (Adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB n=10 Tax=Gimesia TaxID=1649453 RepID=A0A2E7K5C2_9PLAN)

HSP 1 Score: 310 bits (793), Expect = 1.660e-105
Identity = 149/151 (98.68%), Postives = 150/151 (99.34%), Query Frame = 1
Query:    1 AKTFGYVTGAPVHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLLDQGSSPEIRIEDFPRQTLASQVAELTATLLQQETPPASEVWNLTPFYLRKSAAEEKWDAQHNQ 453
            AKTFGYVTGAP+HGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLLDQGS PEIRIEDFPRQTLASQVAELTATLLQQETPPASEVWNLTPFYLRKSAAEEKWDAQHNQ
Sbjct:   91 AKTFGYVTGAPIHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLLDQGSLPEIRIEDFPRQTLASQVAELTATLLQQETPPASEVWNLTPFYLRKSAAEEKWDAQHNQ 241          
BLAST of mRNA_M-pyrifera_M_contig102834.607.1 vs. uniprot
Match: A0A517Q5Z4_9PLAN (tRNA threonylcarbamoyladenosine biosynthesis protein TsaB n=2 Tax=Gimesia panareensis TaxID=2527978 RepID=A0A517Q5Z4_9PLAN)

HSP 1 Score: 219 bits (557), Expect = 1.150e-69
Identity = 107/149 (71.81%), Postives = 124/149 (83.22%), Query Frame = 1
Query:    1 AKTFGYVTGAPVHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLLDQGSSPEIRIEDFPRQTLASQVAELTATLLQQETPPASEVWNLTPFYLRKSAAEEKWDAQH 447
            A+TFGYVTGAPV GIDTF AIALS P ++ ET+V SNAQRGDLFVG+Y R +  +WQQT  I L++I EFS+ALQPGE V GPGIDLLDQ + PEI+I D PR TLA+QVAE+TA LL Q TPP SEVW+LTPFYLRKSAAEEKWDA++
Sbjct:   84 ARTFGYVTGAPVLGIDTFEAIALSSPADLQETWVTSNAQRGDLFVGKYTRIAARQWQQTRQITLREITEFSAALQPGEAVSGPGIDLLDQNALPEIQIVDTPRDTLAAQVAEITARLLTQPTPPPSEVWSLTPFYLRKSAAEEKWDARN 232          
BLAST of mRNA_M-pyrifera_M_contig102834.607.1 vs. uniprot
Match: A6CC68_9PLAN (TsaD domain-containing protein n=8 Tax=Planctomycetaceae TaxID=126 RepID=A6CC68_9PLAN)

HSP 1 Score: 181 bits (459), Expect = 9.160e-55
Identity = 91/150 (60.67%), Postives = 111/150 (74.00%), Query Frame = 1
Query:    1 AKTFGYVTGAPVHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLLDQG--SSPEIRIEDFPRQTLASQVAELTATLLQQETPPASEVWNLTPFYLRKSAAEEKWDAQ 444
            AKTFGYVTG  V GIDTF AIAL  P +I ET+VISNAQRGDLFVG+Y++ S   WQQ   I L+ I EFS+ LQPG+TV GPGI LL +   S P +   +F     A+++AE+TA ++Q+  PP +EVW+LTPFYLRKSAAEEKWDAQ
Sbjct:   91 AKTFGYVTGCTVVGIDTFEAIALKSPPQIKETWVISNAQRGDLFVGKYLKTSERHWQQVTDIGLRSIEEFSNFLQPGDTVSGPGITLLPENLQSDPRLLQPEF-HIPQAAEIAEITARIMQENQPPRNEVWDLTPFYLRKSAAEEKWDAQ 239          
BLAST of mRNA_M-pyrifera_M_contig102834.607.1 vs. uniprot
Match: A0A2E2C8H8_9PLAN (tRNA (Adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB n=4 Tax=Planctomycetaceae TaxID=126 RepID=A0A2E2C8H8_9PLAN)

HSP 1 Score: 179 bits (453), Expect = 8.600e-54
Identity = 94/154 (61.04%), Postives = 113/154 (73.38%), Query Frame = 1
Query:    1 AKTFGYVTGAPVHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLL--DQGSSPEIRIEDFPRQTLASQVAELTATLLQQET---PPASEVWNLTPFYLRKSAAEEKWDAQH 447
            AKTFGYVTG PV GIDTF AIA++ P++ISET+VISNAQRGDLFVG+Y +     WQ T  I L+ I EF  +LQP +TV GPGI LL  D  S+P +  E+F R   A++VAE+TA  LQ+     PP +EVW+L PFYLRKSAAEEKWDAQH
Sbjct:   91 AKTFGYVTGCPVIGIDTFEAIAMNSPSDISETWVISNAQRGDLFVGKYRKTGERCWQNTAEIGLRSIEEFVKSLQPDDTVSGPGIALLPEDFQSAPRLLTEEF-RTPQAAEVAEITANTLQKNAANQPPLNEVWDLIPFYLRKSAAEEKWDAQH 243          
BLAST of mRNA_M-pyrifera_M_contig102834.607.1 vs. uniprot
Match: A0A518IBW5_9PLAN (tRNA threonylcarbamoyladenosine biosynthesis protein TsaB n=1 Tax=Gimesia fumaroli TaxID=2527976 RepID=A0A518IBW5_9PLAN)

HSP 1 Score: 177 bits (449), Expect = 3.170e-53
Identity = 92/153 (60.13%), Postives = 109/153 (71.24%), Query Frame = 1
Query:    1 AKTFGYVTGAPVHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLLDQGSSPEIRI-EDFPRQTLASQVAELTATLLQQETPPA----SEVWNLTPFYLRKSAAEEKWDAQ 444
            AKTFGYVTG PV GID+F AIAL+CP EI ETYVISNAQRGDLFVG+Y R S  +WQQ   I L +I +F   L PGET+ GPG+ LLD  S  ++R+ E   R   A++++ +TA +LQ   P      SE WNLTPFYLRKSAAEEKWDAQ
Sbjct:   84 AKTFGYVTGCPVLGIDSFEAIALNCPPEIQETYVISNAQRGDLFVGKYTRISDSQWQQISEIGLCEIDDFCRLLNPGETISGPGVTLLDASSFSDVRLLEPEYRLPEAAKISLITARILQNCLPKQHEILSETWNLTPFYLRKSAAEEKWDAQ 236          
BLAST of mRNA_M-pyrifera_M_contig102834.607.1 vs. uniprot
Match: A0A517RF27_9PLAN (tRNA threonylcarbamoyladenosine biosynthesis protein TsaB n=1 Tax=Gimesia alba TaxID=2527973 RepID=A0A517RF27_9PLAN)

HSP 1 Score: 177 bits (449), Expect = 3.170e-53
Identity = 90/154 (58.44%), Postives = 111/154 (72.08%), Query Frame = 1
Query:    1 AKTFGYVTGAPVHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLLDQGSSPEIRI-EDFPRQTLASQVAELTATLLQQETPPA----SEVWNLTPFYLRKSAAEEKWDAQH 447
            AKTFGYVT  PV GIDTF AIAL+ P EI ETYVISNAQRGDLFVG+YV+ S  +WQQT  I L ++ +F  +L PGE + GPGI+LL+  S  E+++ E   R  +A+++AE+T  + Q   P      +E WNLTPFYLRKSAAEEKWDAQH
Sbjct:   84 AKTFGYVTNCPVLGIDTFEAIALNSPPEIQETYVISNAQRGDLFVGKYVKLSDNQWQQTSEISLHEMTDFCRSLTPGEAISGPGIELLETTSLSEVQLLEPEYRFPVAAKIAEITGRIHQNSPPEQHESLNETWNLTPFYLRKSAAEEKWDAQH 237          
BLAST of mRNA_M-pyrifera_M_contig102834.607.1 vs. uniprot
Match: A0A517WV28_9PLAN (tRNA threonylcarbamoyladenosine biosynthesis protein TsaB n=2 Tax=Gimesia aquarii TaxID=2527964 RepID=A0A517WV28_9PLAN)

HSP 1 Score: 174 bits (442), Expect = 3.970e-52
Identity = 91/153 (59.48%), Postives = 112/153 (73.20%), Query Frame = 1
Query:    1 AKTFGYVTGAPVHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLLDQGSSPEIRI-EDFPRQTLASQVAELTATLLQQETPPA----SEVWNLTPFYLRKSAAEEKWDAQ 444
            AKTF YVTG PV GIDTF AIAL+CP EISETYVISNAQRGDLFVG+YV+ S  +WQ++  I L +I  F  +L+P +T+ GPGI+LL     PE RI +   R   AS+++ +TA +LQ+  P      +EVW+LTPFYLRKSAAEEKWDAQ
Sbjct:   84 AKTFAYVTGCPVIGIDTFEAIALNCPPEISETYVISNAQRGDLFVGKYVKISEYQWQRSSEIALWEIDRFCHSLKPKDTINGPGIELLQTSLLPECRILQPEYRNPTASKISLITAQILQKSQPAEYASLNEVWDLTPFYLRKSAAEEKWDAQ 236          
BLAST of mRNA_M-pyrifera_M_contig102834.607.1 vs. uniprot
Match: A0A517V8M0_9PLAN (tRNA threonylcarbamoyladenosine biosynthesis protein TsaB n=1 Tax=Gimesia algae TaxID=2527971 RepID=A0A517V8M0_9PLAN)

HSP 1 Score: 174 bits (440), Expect = 6.480e-52
Identity = 92/155 (59.35%), Postives = 110/155 (70.97%), Query Frame = 1
Query:    1 AKTFGYVTGAPVHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLL--DQGSSPEIRIEDFPRQTLASQVAELTATLLQQETP---PASEVWNLTPFYLRKSAAEEKWDAQHN 450
            AKTFGYVTG PV GIDTF AIAL  P+EI+ET+VISNAQRGDLFVG+Y++     WQ T  I L+ I EF  +LQ  +TV GPGI LL  D  S P +   +F R   A++VAE+TA ++Q+      P +EVWNL PFYLRKSAAEEKWDAQ N
Sbjct:   84 AKTFGYVTGCPVLGIDTFEAIALKSPSEITETWVISNAQRGDLFVGKYLKIGERHWQNTSEIELRGIEEFGISLQSSDTVSGPGITLLPDDLPSDPRLLQTEF-RTPHAAEVAEITARMMQEHAAKHSPRNEVWNLVPFYLRKSAAEEKWDAQQN 237          
BLAST of mRNA_M-pyrifera_M_contig102834.607.1 vs. uniprot
Match: UPI001985CE84 (tRNA (Adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB n=1 Tax=Candidatus Saccharimonas sp. TaxID=1917697 RepID=UPI001985CE84)

HSP 1 Score: 111 bits (278), Expect = 1.120e-27
Identity = 61/149 (40.94%), Postives = 82/149 (55.03%), Query Frame = 1
Query:    1 AKTFGYVTGAPVHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLLD-QGSSPEIRIEDFPRQTLASQVAELTA--TLLQQETPPASEV--WNLTPFYLRKSAAEEK 432
            AKTF Y TG     +DTF AIA +CP ++ + ++I NAQRGD FVGRYVR+ T  W+ T PI      E+ +A    ET+ GPG+   D Q     +  +D   +  AS +A L     L +    P+ +   W   PFY+R SAAEEK
Sbjct:   64 AKTFAYATGCQFVAVDTFEAIADNCPIQVRDVWIIENAQRGDFFVGRYVRDETDSWRPTGPIQTVAAEEWLAARSADETITGPGLPYGDLQAVRARLLTDDAVARPRASAIARLGQHRLLARDACSPSDDFDFWKAVPFYIRPSAAEEK 212          
BLAST of mRNA_M-pyrifera_M_contig102834.607.1 vs. uniprot
Match: A0A3B1DY74_9ZZZZ (tRNA threonylcarbamoyladenosine biosynthesis protein TsaB n=1 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A3B1DY74_9ZZZZ)

HSP 1 Score: 107 bits (266), Expect = 8.480e-26
Identity = 62/158 (39.24%), Postives = 90/158 (56.96%), Query Frame = 1
Query:    1 AKTFGYVTGAPVHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVRNSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLLDQGSSPE-------IRIEDFPRQTLASQVAELTATLLQQETPPASEVWNLTPFYLRKSAAEEKWDAQHNQ 453
            AKTF Y TG  +  +DTF AIA + P +I +  VISNAQRG+LFVG+Y+RNS G + +T  I +    EF  +L    T+ G G+ L+++ +  +       I  E++  Q  A+Q+  L   L+QQ     +  W L P Y+R SAAEEK  A+  +
Sbjct:   80 AKTFAYATGCLLVAVDTFLAIASNAPNDIEKIEVISNAQRGELFVGKYIRNSEGNFFRTDEIEMIGTEEFIKSLSSKTTIAGSGVRLIEEEAKKQGDTLQGNILPEEY-WQPQATQICLLGEQLIQQNE--TANPWTLAPHYIRHSAAEEKRAAEEGK 234          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig102834.607.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2E7K5C2_9PLAN1.660e-10598.68tRNA (Adenosine(37)-N6)-threonylcarbamoyltransfera... [more]
A0A517Q5Z4_9PLAN1.150e-6971.81tRNA threonylcarbamoyladenosine biosynthesis prote... [more]
A6CC68_9PLAN9.160e-5560.67TsaD domain-containing protein n=8 Tax=Planctomyce... [more]
A0A2E2C8H8_9PLAN8.600e-5461.04tRNA (Adenosine(37)-N6)-threonylcarbamoyltransfera... [more]
A0A518IBW5_9PLAN3.170e-5360.13tRNA threonylcarbamoyladenosine biosynthesis prote... [more]
A0A517RF27_9PLAN3.170e-5358.44tRNA threonylcarbamoyladenosine biosynthesis prote... [more]
A0A517WV28_9PLAN3.970e-5259.48tRNA threonylcarbamoyladenosine biosynthesis prote... [more]
A0A517V8M0_9PLAN6.480e-5259.35tRNA threonylcarbamoyladenosine biosynthesis prote... [more]
UPI001985CE841.120e-2740.94tRNA (Adenosine(37)-N6)-threonylcarbamoyltransfera... [more]
A0A3B1DY74_9ZZZZ8.480e-2639.24tRNA threonylcarbamoyladenosine biosynthesis prote... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig102834contigM-pyrifera_M_contig102834:201..656 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score181.0
Seed ortholog evalue4.1e-43
Seed eggNOG ortholog344747.PM8797T_31750
Preferred nameyeaZ
KEGG koko:K14742
Hectar predicted targeting categoryother localisation
GOsGO:0002949,GO:0003674,GO:0003824,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006139,GO:0006396,GO:0006399,GO:0006400,GO:0006508,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0008233,GO:0008237,GO:0009451,GO:0009987,GO:0010467,GO:0016070,GO:0016787,GO:0019538,GO:0034470,GO:0034641,GO:0034660,GO:0042802,GO:0043170,GO:0043412,GO:0044237,GO:0044238,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0070011,GO:0070525,GO:0071704,GO:0090304,GO:0140096,GO:1901360,GO:1901564
EggNOG free text desc.Glycoprotease family
EggNOG OGs2J0R2@203682,COG1214@1,COG1214@2
COG Functional cat.O
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03016
Exons1
Model size456
Cds size456
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815532.6151721-CDS-M-pyrifera_M_contig102834:200..6561622815532.6151721-CDS-M-pyrifera_M_contig102834:200..656Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102834 201..656 -
1692277452.284614-CDS-M-pyrifera_M_contig102834:200..6561692277452.284614-CDS-M-pyrifera_M_contig102834:200..656Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig102834 201..656 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig102834.607.1prot_M-pyrifera_M_contig102834.607.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig102834 201..656 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig102834.607.1

>prot_M-pyrifera_M_contig102834.607.1 ID=prot_M-pyrifera_M_contig102834.607.1|Name=mRNA_M-pyrifera_M_contig102834.607.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=152bp
AKTFGYVTGAPVHGIDTFAAIALSCPTEISETYVISNAQRGDLFVGRYVR
NSTGEWQQTVPIHLQDIHEFSSALQPGETVCGPGIDLLDQGSSPEIRIED
FPRQTLASQVAELTATLLQQETPPASEVWNLTPFYLRKSAAEEKWDAQHN
Q*
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mRNA from alignment at M-pyrifera_M_contig102834:201..656-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig102834.607.1 ID=mRNA_M-pyrifera_M_contig102834.607.1|Name=mRNA_M-pyrifera_M_contig102834.607.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=456bp|location=Sequence derived from alignment at M-pyrifera_M_contig102834:201..656- (Macrocystis pyrifera P11B4 male)
GCCAAAACCTTTGGCTACGTGACAGGTGCTCCCGTACATGGCATCGATAC CTTTGCAGCCATCGCACTCAGCTGTCCGACTGAAATTTCAGAGACCTATG TGATCTCCAACGCCCAGCGGGGCGATCTATTCGTCGGCAGATATGTCCGC AATTCCACAGGAGAATGGCAACAGACAGTTCCCATTCATCTGCAGGACAT CCACGAATTCAGCAGTGCACTTCAGCCCGGTGAAACCGTCTGCGGTCCCG GAATCGATCTGCTCGACCAAGGCAGCTCACCGGAAATCCGAATCGAAGAT TTTCCCCGTCAGACACTCGCCTCCCAGGTCGCAGAATTGACGGCCACACT CCTGCAACAGGAGACTCCACCGGCCAGTGAAGTCTGGAACCTGACTCCCT TCTATCTTCGCAAAAGTGCAGCGGAAGAGAAGTGGGATGCACAACATAAT CAGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig102834:201..656-

>mRNA_M-pyrifera_M_contig102834.607.1 ID=mRNA_M-pyrifera_M_contig102834.607.1|Name=mRNA_M-pyrifera_M_contig102834.607.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=912bp|location=Sequence derived from alignment at M-pyrifera_M_contig102834:201..656- (Macrocystis pyrifera P11B4 male)
GCCAAAACCTTTGGCTACGTGACAGGTGCTCCCGTACATGGCATCGATAC
CTTTGCAGCCATCGCACTCAGCTGTCCGACTGAAATTTCAGAGACCTATG
TGATCTCCAACGCCCAGCGGGGCGATCTATTCGTCGGCAGATATGTCCGC
AATTCCACAGGAGAATGGCAACAGACAGTTCCCATTCATCTGCAGGACAT
CCACGAATTCAGCAGTGCACTTCAGCCCGGTGAAACCGTCTGCGGTCCCG
GAATCGATCTGCTCGACCAAGGCAGCTCACCGGAAATCCGAATCGAAGAT
TTTCCCCGTCAGACACTCGCCTCCCAGGTCGCAGAATTGACGGCCACACT
CCTGCAACAGGAGACTCCACCGGCCAGTGAAGTCTGGAACCTGACTCCCT
TCTATCTTCGCAAAAGTGCAGCGGAAGAGAAGTGGGATGCACAACATAAT
CAGTAAGCCAAAACCTTTGGCTACGTGACAGGTGCTCCCGTACATGGCAT
CGATACCTTTGCAGCCATCGCACTCAGCTGTCCGACTGAAATTTCAGAGA
CCTATGTGATCTCCAACGCCCAGCGGGGCGATCTATTCGTCGGCAGATAT
GTCCGCAATTCCACAGGAGAATGGCAACAGACAGTTCCCATTCATCTGCA
GGACATCCACGAATTCAGCAGTGCACTTCAGCCCGGTGAAACCGTCTGCG
GTCCCGGAATCGATCTGCTCGACCAAGGCAGCTCACCGGAAATCCGAATC
GAAGATTTTCCCCGTCAGACACTCGCCTCCCAGGTCGCAGAATTGACGGC
CACACTCCTGCAACAGGAGACTCCACCGGCCAGTGAAGTCTGGAACCTGA
CTCCCTTCTATCTTCGCAAAAGTGCAGCGGAAGAGAAGTGGGATGCACAA
CATAATCAGTAA
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