mRNA_M-pyrifera_M_contig100225.54.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig100225.54.1
Unique NamemRNA_M-pyrifera_M_contig100225.54.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig100225.54.1 vs. uniprot
Match: A0A5C6B0Y5_9BACT (RNA polymerase sigma factor SigD n=1 Tax=Rhodopirellula pilleata TaxID=2714738 RepID=A0A5C6B0Y5_9BACT)

HSP 1 Score: 314 bits (805), Expect = 2.480e-107
Identity = 155/186 (83.33%), Postives = 173/186 (93.01%), Query Frame = 1
Query:    1 NDPASEEAWGEFAAVYRPLVIRVARAKGLQHADADDLAQNVFSAVGKAIGSFTSTGDGSFRRWLYRITRNLVVNQLTRGPFSGRRGPIGSGDSDVQKLLNQTPELDDQTATLFQLEYRRARFQQAADKVRNQFSDATWQCFWLTSVENQPAERVAAALGKTPGAVRVARCRVLAKIRQETQTDTTE 558
            NDPASEEAW EFAAVYRPLVIRVARAKGLQHADADDLAQ+V +AVG AIGSFT+TG+GSFRRWLY+ITRNLVVNQLTRGPFSG+RGPIGSG+SDVQK L+Q P   DQTATLFQLEY+R RFQQAA KV++QFS++TWQCFWLTSVENQ AE+VA+++GKT GAVRVARCRVLAKIR+ETQTDT E
Sbjct:   15 NDPASEEAWYEFAAVYRPLVIRVARAKGLQHADADDLAQDVLAAVGNAIGSFTTTGEGSFRRWLYQITRNLVVNQLTRGPFSGQRGPIGSGNSDVQKFLSQAPAPGDQTATLFQLEYQRTRFQQAATKVKDQFSESTWQCFWLTSVENQSAEQVASSIGKTAGAVRVARCRVLAKIREETQTDTNE 200          
BLAST of mRNA_M-pyrifera_M_contig100225.54.1 vs. uniprot
Match: A0A518GD63_9BACT (ECF RNA polymerase sigma factor SigE n=1 Tax=Aureliella helgolandensis TaxID=2527968 RepID=A0A518GD63_9BACT)

HSP 1 Score: 301 bits (771), Expect = 3.750e-102
Identity = 149/186 (80.11%), Postives = 168/186 (90.32%), Query Frame = 1
Query:    1 NDPASEEAWGEFAAVYRPLVIRVARAKGLQHADADDLAQNVFSAVGKAIGSFTSTGDGSFRRWLYRITRNLVVNQLTRGPFSGRRGPIGSGDSDVQKLLNQTPELDDQTATLFQLEYRRARFQQAADKVRNQFSDATWQCFWLTSVENQPAERVAAALGKTPGAVRVARCRVLAKIRQETQTDTTE 558
            NDPASEEAW EFAAVYRPLVIRVARAKGLQ+ADADD+AQ+V SAVGK++ SFTS+G+GSFRRWLY+ITRNLVVNQL RGPFSGRRGPIGSGDSDVQ LL+Q P  +DQTATLF++EYRRARFQQAA+KV+NQFS+ TWQCFW TSVENQ  E VA+++GKT GAVRVARCRVLAKIR+E Q D  E
Sbjct:   15 NDPASEEAWTEFAAVYRPLVIRVARAKGLQNADADDVAQDVLSAVGKSLESFTSSGEGSFRRWLYQITRNLVVNQLMRGPFSGRRGPIGSGDSDVQYLLSQAPTPEDQTATLFRIEYRRARFQQAAEKVKNQFSELTWQCFWQTSVENQSVEHVASSIGKTVGAVRVARCRVLAKIREEIQNDIDE 200          
BLAST of mRNA_M-pyrifera_M_contig100225.54.1 vs. uniprot
Match: A0A5C6ASX2_9BACT (RNA polymerase sigma factor RpoE n=2 Tax=Stieleria varia TaxID=2528005 RepID=A0A5C6ASX2_9BACT)

HSP 1 Score: 236 bits (601), Expect = 7.760e-76
Identity = 117/182 (64.29%), Postives = 138/182 (75.82%), Query Frame = 1
Query:    1 NDPASEEAWGEFAAVYRPLVIRVARAKGLQHADADDLAQNVFSAVGKAIGSFTSTGDGSFRRWLYRITRNLVVNQLTRGPFSGRRGPIGSGDSDVQKLLNQTPELDDQTATLFQLEYRRARFQQAADKVRNQFSDATWQCFWLTSVENQPAERVAAALGKTPGAVRVARCRVLAKIRQETQT 546
            NDP++ +AW EF  +YRPLV RVAR KGLQHADA+DLAQ V + V  AI SF    DGSFR WL+ ITRNLVVN LTRGP S + GPIGSGDS VQ++L Q P  +  TATLFQLEYRR RFQQAA K+++QF++ TWQ FW TSV+ Q    VA  L +TPGA+R+ARCRVLA+IR E QT
Sbjct:   45 NDPSASDAWAEFDGLYRPLVYRVARGKGLQHADAEDLAQQVLAVVNGAIDSFDPDADGSFRGWLFTITRNLVVNHLTRGPHSRKHGPIGSGDSKVQQMLMQHPSDESHTATLFQLEYRRGRFQQAARKLKSQFNEETWQAFWRTSVDGQSISDVAEQLARTPGAIRMARCRVLARIRGEVQT 226          
BLAST of mRNA_M-pyrifera_M_contig100225.54.1 vs. uniprot
Match: A0A5C6DRX9_9BACT (ECF RNA polymerase sigma factor SigE n=1 Tax=Novipirellula artificiosorum TaxID=2528016 RepID=A0A5C6DRX9_9BACT)

HSP 1 Score: 231 bits (589), Expect = 1.810e-74
Identity = 114/178 (64.04%), Postives = 142/178 (79.78%), Query Frame = 1
Query:    1 NDPASEEAWGEFAAVYRPLVIRVARAKGLQHADADDLAQNVFSAVGKAIGSFTSTGDGSFRRWLYRITRNLVVNQLTRGPFSGRRGPIGSGDSDVQKLLNQTPELDDQTATLFQLEYRRARFQQAADKVRNQFSDATWQCFWLTSVENQPAERVAAALGKTPGAVRVARCRVLAKIRQ 534
            +DPAS+EAW EFA +YRP+++RVA+A+GLQHADADDLAQ+V SAVG+ +GSF STG GSFRRWL +ITRNLVVN LTR      +GPIGSGDSDVQ++L Q P  + +TATLFQLE RR  FQQAA+K++ +FS++TW  FWLTSVE    +  A  L K+ G++RVARCRVLA+ RQ
Sbjct:   16 SDPASQEAWTEFATIYRPIIVRVAQARGLQHADADDLAQDVLSAVGRRVGSFQSTGTGSFRRWLCQITRNLVVNHLTRS-----KGPIGSGDSDVQQMLLQQPSPEGETATLFQLEVRRFEFQQAAEKLQAEFSESTWMAFWLTSVEQLSIDEAANRLSKSAGSIRVARCRVLARFRQ 188          
BLAST of mRNA_M-pyrifera_M_contig100225.54.1 vs. uniprot
Match: A0A5M6CT70_9BACT (Sigma-70 family RNA polymerase sigma factor n=1 Tax=Roseiconus nitratireducens TaxID=2605748 RepID=A0A5M6CT70_9BACT)

HSP 1 Score: 231 bits (589), Expect = 2.000e-74
Identity = 118/183 (64.48%), Postives = 139/183 (75.96%), Query Frame = 1
Query:    1 NDPASEEAWGEFAAVYRPLVIRVARAKGLQHADADDLAQNVFSAVGKAIGSFTSTGDGSFRRWLYRITRNLVVNQLTRGPFSGRRGPIGSGDSDVQKLLNQTPELDDQTATLFQLEYRRARFQQAADKVRNQFSDATWQCFWLTSVENQPAERVAAALGKTPGAVRVARCRVLAKIRQETQTD 549
             +P ++EAW +F A+YRPLVIRVA+AKGLQHADA+DLAQ V   V + I SF   GDGSFR WL+R+TRNLVVN+LTR       GP GSGDS VQK L+Q P  DD TATLF LEYRRARF+QAA  V++QF+  TWQ FWLT++E QP + VA  LGKTPGAVRVARCRV+AK+R E   D
Sbjct:   16 KNPHADEAWTDFDALYRPLVIRVAQAKGLQHADAEDLAQEVLMTVRRTIESFEHRGDGSFRSWLFRVTRNLVVNRLTR-----TSGPTGSGDSKVQKALSQQPADDDPTATLFLLEYRRARFKQAAANVKSQFALTTWQAFWLTAIEEQPVDHVARKLGKTPGAVRVARCRVIAKLRDEVAID 193          
BLAST of mRNA_M-pyrifera_M_contig100225.54.1 vs. uniprot
Match: UPI001E6312D4 (sigma-70 family RNA polymerase sigma factor n=1 Tax=Stieleria varia TaxID=2528005 RepID=UPI001E6312D4)

HSP 1 Score: 229 bits (584), Expect = 9.740e-74
Identity = 113/179 (63.13%), Postives = 141/179 (78.77%), Query Frame = 1
Query:   13 SEEAWGEFAAVYRPLVIRVARAKGLQHADADDLAQNVFSAVGKAIGSFTSTGDGSFRRWLYRITRNLVVNQLTRGPFSGRRGPIGSGDSDVQKLLNQTPELDDQTATLFQLEYRRARFQQAADKVRNQFSDATWQCFWLTSVENQPAERVAAALGKTPGAVRVARCRVLAKIRQETQTD 549
            S++AW EF  +YRPLV+RVA AKGLQHADADDLAQ+V + VGKAI SF    DGSFR WL++ITRNL VNQLTRGPFSG RGPIGSGDSDV ++L Q P  D+ T TLF+ E+RR RFQQAA+ ++ +F+++TW  FWLT+V+ +  + VA  LG T G+VRVARCRVLAK++   Q+D
Sbjct:   19 SDDAWAEFVELYRPLVVRVALAKGLQHADADDLAQDVLATVGKAINSFDPLADGSFRGWLFKITRNLCVNQLTRGPFSGNRGPIGSGDSDVHRMLLQQPA-DEATVTLFETEHRRLRFQQAAEMLQARFTESTWLSFWLTAVDGRSIQSVADELGMTEGSVRVARCRVLAKLKDTLQSD 196          
BLAST of mRNA_M-pyrifera_M_contig100225.54.1 vs. uniprot
Match: A0A5C6B744_9BACT (ECF RNA polymerase sigma factor SigE n=1 Tax=Stieleria varia TaxID=2528005 RepID=A0A5C6B744_9BACT)

HSP 1 Score: 229 bits (584), Expect = 4.350e-72
Identity = 113/179 (63.13%), Postives = 141/179 (78.77%), Query Frame = 1
Query:   13 SEEAWGEFAAVYRPLVIRVARAKGLQHADADDLAQNVFSAVGKAIGSFTSTGDGSFRRWLYRITRNLVVNQLTRGPFSGRRGPIGSGDSDVQKLLNQTPELDDQTATLFQLEYRRARFQQAADKVRNQFSDATWQCFWLTSVENQPAERVAAALGKTPGAVRVARCRVLAKIRQETQTD 549
            S++AW EF  +YRPLV+RVA AKGLQHADADDLAQ+V + VGKAI SF    DGSFR WL++ITRNL VNQLTRGPFSG RGPIGSGDSDV ++L Q P  D+ T TLF+ E+RR RFQQAA+ ++ +F+++TW  FWLT+V+ +  + VA  LG T G+VRVARCRVLAK++   Q+D
Sbjct:  139 SDDAWAEFVELYRPLVVRVALAKGLQHADADDLAQDVLATVGKAINSFDPLADGSFRGWLFKITRNLCVNQLTRGPFSGNRGPIGSGDSDVHRMLLQQPA-DEATVTLFETEHRRLRFQQAAEMLQARFTESTWLSFWLTAVDGRSIQSVADELGMTEGSVRVARCRVLAKLKDTLQSD 316          
BLAST of mRNA_M-pyrifera_M_contig100225.54.1 vs. uniprot
Match: A0A518FZU6_9BACT (ECF RNA polymerase sigma factor SigE n=2 Tax=Aureliella helgolandensis TaxID=2527968 RepID=A0A518FZU6_9BACT)

HSP 1 Score: 216 bits (549), Expect = 2.630e-68
Identity = 110/179 (61.45%), Postives = 137/179 (76.54%), Query Frame = 1
Query:    7 PASEEAWGEFAAVYRPLVIRVARAKGLQHADADDLAQNVFSAVGKAIGSFTSTGDGSFRRWLYRITRNLVVNQLTRGPFSGRRGPIGSGDSDVQKLLNQTPELDDQTATLFQLEYRRARFQQAADKVRNQFSDATWQCFWLTSVENQPAERVAAALGKTPGAVRVARCRVLAKIRQETQ 543
            PAS+EAW EF  +YRPLVIRVARAKGLQHADA+DLAQ+V + VG+A+ SF    +GSFR WL++ITRNL +N LTRG     RGPIGSGDSDVQ++L Q P  D+ T TLF  E+RR RF+QAA+ ++  F ++TW  FW T+VE +  E VA ALGKT G+VRVARCRVLAK+++  Q
Sbjct:   30 PASQEAWTEFVELYRPLVIRVARAKGLQHADAEDLAQDVLATVGRAVDSFDPAAEGSFRGWLFKITRNLCINHLTRG-----RGPIGSGDSDVQRILLQLPA-DEATVTLFATEHRRMRFRQAAEALKPSFQESTWLSFWWTTVEGRSIESVAEALGKTNGSVRVARCRVLAKLKEALQ 202          
BLAST of mRNA_M-pyrifera_M_contig100225.54.1 vs. uniprot
Match: Q7UEM7_RHOBA (Probable extracytoplasmic function alternative sigma factor n=9 Tax=Rhodopirellula TaxID=265488 RepID=Q7UEM7_RHOBA)

HSP 1 Score: 214 bits (546), Expect = 6.600e-68
Identity = 108/186 (58.06%), Postives = 141/186 (75.81%), Query Frame = 1
Query:    1 NDPASEEAWGEFAAVYRPLVIRVARAKGLQHADADDLAQNVFSAVGKAIGSFTSTGDGSFRRWLYRITRNLVVNQLTRGPFSGRRGPIGSGDSDVQKLLNQTPELDDQTATLFQLEYRRARFQQAADKVRNQFSDATWQCFWLTSVENQPAERVAAALGKTPGAVRVARCRVLAKIRQETQTDTTE 558
            N+PASEE W EF ++YRPLV+RVA AKGLQHADA+DL Q+VF+AV +++  F S G GSFR WL+RITRNLVVN LTR      +GPIGSGDS VQ+ L Q PE +++TATLF  E RR +FQ AAD+V+     ATWQ FW+T+VE +    VA +LGK+ G+VR+A+CRVLA+++QE Q  +++
Sbjct:   16 NEPASEEVWTEFVSIYRPLVVRVALAKGLQHADAEDLTQDVFAAVRRSLIKFESRGSGSFRAWLFRITRNLVVNHLTRS-----KGPIGSGDSHVQQWLMQQPERENETATLFDHELRRQQFQLAADRVQPHVEPATWQSFWMTAVEGKSISCVAESLGKSEGSVRMAKCRVLARLQQEAQAISSQ 196          
BLAST of mRNA_M-pyrifera_M_contig100225.54.1 vs. uniprot
Match: A0A518HTB4_9BACT (RNA polymerase sigma factor RpoE n=1 Tax=Stieleria neptunia TaxID=2527979 RepID=A0A518HTB4_9BACT)

HSP 1 Score: 211 bits (538), Expect = 1.080e-66
Identity = 104/181 (57.46%), Postives = 135/181 (74.59%), Query Frame = 1
Query:    1 NDPASEEAWGEFAAVYRPLVIRVARAKGLQHADADDLAQNVFSAVGKAIGSFTSTGDGSFRRWLYRITRNLVVNQLTRGPFSGRRGPIGSGDSDVQKLLNQTPELDDQTATLFQLEYRRARFQQAADKVRNQFSDATWQCFWLTSVENQPAERVAAALGKTPGAVRVARCRVLAKIRQETQ 543
            N PASEE W EF  +YRPLV+RV  AKGLQHADA+DLAQ+VF+AV +++G+FT  G GSFR WL+RITRNLVVN LTR      +GP+GSGDS + +LL Q P+  ++TATLF  E RR +F  AA++VR +F  ATW  FW T+V  +    VA++LGK+ GAVR+A+CRV A+++QE Q
Sbjct:   16 NQPASEEVWMEFVTIYRPLVVRVGLAKGLQHADAEDLAQDVFAAVRRSLGTFTKRGQGSFRAWLFRITRNLVVNHLTRS-----KGPVGSGDSQMNRLLMQQPDRQNETATLFDHELRRQQFHLAAERVRPKFEPATWLAFWRTAVGGESISTVASSLGKSEGAVRMAKCRVFARLQQEAQ 191          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig100225.54.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5C6B0Y5_9BACT2.480e-10783.33RNA polymerase sigma factor SigD n=1 Tax=Rhodopire... [more]
A0A518GD63_9BACT3.750e-10280.11ECF RNA polymerase sigma factor SigE n=1 Tax=Aurel... [more]
A0A5C6ASX2_9BACT7.760e-7664.29RNA polymerase sigma factor RpoE n=2 Tax=Stieleria... [more]
A0A5C6DRX9_9BACT1.810e-7464.04ECF RNA polymerase sigma factor SigE n=1 Tax=Novip... [more]
A0A5M6CT70_9BACT2.000e-7464.48Sigma-70 family RNA polymerase sigma factor n=1 Ta... [more]
UPI001E6312D49.740e-7463.13sigma-70 family RNA polymerase sigma factor n=1 Ta... [more]
A0A5C6B744_9BACT4.350e-7263.13ECF RNA polymerase sigma factor SigE n=1 Tax=Stiel... [more]
A0A518FZU6_9BACT2.630e-6861.45ECF RNA polymerase sigma factor SigE n=2 Tax=Aurel... [more]
Q7UEM7_RHOBA6.600e-6858.06Probable extracytoplasmic function alternative sig... [more]
A0A518HTB4_9BACT1.080e-6657.46RNA polymerase sigma factor RpoE n=1 Tax=Stieleria... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig100225contigM-pyrifera_M_contig100225:121..681 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score219.2
Seed ortholog evalue1.7e-54
Seed eggNOG ortholog243090.RB11242
KEGG koko:K03088
Hectar predicted targeting categoryother localisation
EggNOG free text desc.COG1595 DNA-directed RNA polymerase specialized sigma
EggNOG OGs2J2T2@203682,COG1595@1,COG1595@2
COG Functional cat.K
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03021
Exons1
Model size561
Cds size561
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815497.6669164-CDS-M-pyrifera_M_contig100225:120..6811622815497.6669164-CDS-M-pyrifera_M_contig100225:120..681Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100225 121..681 -
1692277438.6235921-CDS-M-pyrifera_M_contig100225:120..6811692277438.6235921-CDS-M-pyrifera_M_contig100225:120..681Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig100225 121..681 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig100225.54.1prot_M-pyrifera_M_contig100225.54.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig100225 121..681 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig100225.54.1

>prot_M-pyrifera_M_contig100225.54.1 ID=prot_M-pyrifera_M_contig100225.54.1|Name=mRNA_M-pyrifera_M_contig100225.54.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=187bp
NDPASEEAWGEFAAVYRPLVIRVARAKGLQHADADDLAQNVFSAVGKAIG
SFTSTGDGSFRRWLYRITRNLVVNQLTRGPFSGRRGPIGSGDSDVQKLLN
QTPELDDQTATLFQLEYRRARFQQAADKVRNQFSDATWQCFWLTSVENQP
AERVAAALGKTPGAVRVARCRVLAKIRQETQTDTTE*
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mRNA from alignment at M-pyrifera_M_contig100225:121..681-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig100225.54.1 ID=mRNA_M-pyrifera_M_contig100225.54.1|Name=mRNA_M-pyrifera_M_contig100225.54.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=561bp|location=Sequence derived from alignment at M-pyrifera_M_contig100225:121..681- (Macrocystis pyrifera P11B4 male)
AATGATCCGGCTTCGGAGGAAGCCTGGGGTGAGTTTGCGGCTGTCTATCG ACCGCTAGTCATTCGGGTTGCCAGAGCCAAAGGACTGCAACATGCCGATG CGGATGATCTTGCTCAAAATGTTTTTTCAGCCGTCGGAAAAGCGATCGGA TCTTTCACATCGACTGGCGATGGATCGTTTCGACGATGGCTGTACCGCAT CACACGTAACCTCGTTGTAAATCAATTGACTCGCGGCCCATTTTCCGGCC GACGAGGCCCAATCGGCAGCGGCGACAGTGACGTGCAAAAACTATTGAAC CAAACCCCCGAACTGGATGATCAGACGGCAACATTGTTCCAGCTGGAATA TCGACGAGCCCGATTTCAACAGGCTGCCGACAAGGTCCGAAATCAATTTA GCGATGCGACTTGGCAGTGTTTCTGGTTGACGTCCGTCGAAAACCAGCCC GCCGAACGGGTCGCCGCTGCATTAGGCAAGACTCCTGGGGCTGTGCGTGT GGCACGATGCCGTGTGCTGGCAAAGATCCGCCAAGAAACCCAAACCGATA CAACAGAGTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig100225:121..681-

>mRNA_M-pyrifera_M_contig100225.54.1 ID=mRNA_M-pyrifera_M_contig100225.54.1|Name=mRNA_M-pyrifera_M_contig100225.54.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1122bp|location=Sequence derived from alignment at M-pyrifera_M_contig100225:121..681- (Macrocystis pyrifera P11B4 male)
AATGATCCGGCTTCGGAGGAAGCCTGGGGTGAGTTTGCGGCTGTCTATCG
ACCGCTAGTCATTCGGGTTGCCAGAGCCAAAGGACTGCAACATGCCGATG
CGGATGATCTTGCTCAAAATGTTTTTTCAGCCGTCGGAAAAGCGATCGGA
TCTTTCACATCGACTGGCGATGGATCGTTTCGACGATGGCTGTACCGCAT
CACACGTAACCTCGTTGTAAATCAATTGACTCGCGGCCCATTTTCCGGCC
GACGAGGCCCAATCGGCAGCGGCGACAGTGACGTGCAAAAACTATTGAAC
CAAACCCCCGAACTGGATGATCAGACGGCAACATTGTTCCAGCTGGAATA
TCGACGAGCCCGATTTCAACAGGCTGCCGACAAGGTCCGAAATCAATTTA
GCGATGCGACTTGGCAGTGTTTCTGGTTGACGTCCGTCGAAAACCAGCCC
GCCGAACGGGTCGCCGCTGCATTAGGCAAGACTCCTGGGGCTGTGCGTGT
GGCACGATGCCGTGTGCTGGCAAAGATCCGCCAAGAAACCCAAACCGATA
CAACAGAGTAGAATGATCCGGCTTCGGAGGAAGCCTGGGGTGAGTTTGCG
GCTGTCTATCGACCGCTAGTCATTCGGGTTGCCAGAGCCAAAGGACTGCA
ACATGCCGATGCGGATGATCTTGCTCAAAATGTTTTTTCAGCCGTCGGAA
AAGCGATCGGATCTTTCACATCGACTGGCGATGGATCGTTTCGACGATGG
CTGTACCGCATCACACGTAACCTCGTTGTAAATCAATTGACTCGCGGCCC
ATTTTCCGGCCGACGAGGCCCAATCGGCAGCGGCGACAGTGACGTGCAAA
AACTATTGAACCAAACCCCCGAACTGGATGATCAGACGGCAACATTGTTC
CAGCTGGAATATCGACGAGCCCGATTTCAACAGGCTGCCGACAAGGTCCG
AAATCAATTTAGCGATGCGACTTGGCAGTGTTTCTGGTTGACGTCCGTCG
AAAACCAGCCCGCCGAACGGGTCGCCGCTGCATTAGGCAAGACTCCTGGG
GCTGTGCGTGTGGCACGATGCCGTGTGCTGGCAAAGATCCGCCAAGAAAC
CCAAACCGATACAACAGAGTAG
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