mRNA_M-pyrifera_M_contig125577.5174.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig125577.5174.1
Unique NamemRNA_M-pyrifera_M_contig125577.5174.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig125577.5174.1 vs. uniprot
Match: A0A3D4UKK0_9BACT (CusA/CzcA family heavy metal efflux RND transporter (Fragment) n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A3D4UKK0_9BACT)

HSP 1 Score: 315 bits (806), Expect = 2.550e-102
Identity = 169/170 (99.41%), Postives = 170/170 (100.00%), Query Frame = 1 
Query:    1 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 510
            VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNA+REVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD
Sbjct:  413 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNASREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 582
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BLAST of mRNA_M-pyrifera_M_contig125577.5174.1 vs. uniprot
Match: A0A2D9C3G8_9BACT (CusA/CzcA family heavy metal efflux RND transporter (Fragment) n=2 Tax=Phycisphaerae TaxID=666505 RepID=A0A2D9C3G8_9BACT)

HSP 1 Score: 317 bits (811), Expect = 3.850e-101
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 1 
Query:    1 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 510
            VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD
Sbjct:  583 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 752
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BLAST of mRNA_M-pyrifera_M_contig125577.5174.1 vs. uniprot
Match: A0A812XWB5_9DINO (HelA protein n=1 Tax=Symbiodinium sp. CCMP2592 TaxID=631055 RepID=A0A812XWB5_9DINO)

HSP 1 Score: 317 bits (811), Expect = 5.290e-97
Identity = 170/170 (100.00%), Postives = 170/170 (100.00%), Query Frame = 1 
Query:    1 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 510
            VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD
Sbjct: 4072 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 4241
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BLAST of mRNA_M-pyrifera_M_contig125577.5174.1 vs. uniprot
Match: A0A2E2N5R6_9BACT (CusA/CzcA family heavy metal efflux RND transporter (Fragment) n=1 Tax=Phycisphaerae bacterium TaxID=2026778 RepID=A0A2E2N5R6_9BACT)

HSP 1 Score: 297 bits (761), Expect = 8.670e-94
Identity = 156/170 (91.76%), Postives = 164/170 (96.47%), Query Frame = 1 
Query:    1 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 510
            VESDRLATEVEKQLVEI+GVRSVSRRTGRAERDEHAEPVSNSE+EVTVDAG  R EVR++IDRIL S+PGITTMVGQPIEHRLSHVLSGTPAA+AINVFGE+L+ELRRVAK IEGELQSLPGARDVNANREVMITSLPIRYRHAELAA GLSPADAAEQVREAIYGEVVD
Sbjct:  288 VESDRLATEVEKQLVEIEGVRSVSRRTGRAERDEHAEPVSNSEIEVTVDAGSDRFEVREDIDRILGSVPGITTMVGQPIEHRLSHVLSGTPAAIAINVFGENLTELRRVAKEIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAAGLSPADAAEQVREAIYGEVVD 457
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BLAST of mRNA_M-pyrifera_M_contig125577.5174.1 vs. uniprot
Match: A0A6M8VXQ9_9BACT (Efflux RND transporter permease subunit n=2 Tax=Planctomycetes TaxID=203682 RepID=A0A6M8VXQ9_9BACT)

HSP 1 Score: 293 bits (749), Expect = 5.310e-90
Identity = 153/170 (90.00%), Postives = 163/170 (95.88%), Query Frame = 1 
Query:    1 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 510
            VESDRLATEVEKQLVEI+GVRSVSRRTGRAERDEHAEPVSNSE+EVTVD G  R EVR++IDRIL ++PGITTMVGQPIEHRLSHVLSGTPAA+AINVFGEDL+ELR VAKAIE ELQSLPGARDVNANREV+ITSLPIRYRHAELAAVGLSPADAAEQVREA+YGEVVD
Sbjct:  583 VESDRLATEVEKQLVEIEGVRSVSRRTGRAERDEHAEPVSNSEIEVTVDEGSDRFEVREQIDRILGAVPGITTMVGQPIEHRLSHVLSGTPAAIAINVFGEDLTELRDVAKAIEAELQSLPGARDVNANREVLITSLPIRYRHAELAAVGLSPADAAEQVREALYGEVVD 752
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BLAST of mRNA_M-pyrifera_M_contig125577.5174.1 vs. uniprot
Match: A0A7X0H3Y7_9BACT (HME family heavy-metal exporter n=1 Tax=Algisphaera agarilytica TaxID=1385975 RepID=A0A7X0H3Y7_9BACT)

HSP 1 Score: 290 bits (741), Expect = 6.290e-89
Identity = 151/170 (88.82%), Postives = 162/170 (95.29%), Query Frame = 1 
Query:    1 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 510
            +ESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSE+EVTV+ GR R EVR+ IDR+L +IPGITTMVGQPIEHRLSHVLSGTPAA+ INV+GE+LSELRRVAK +EGELQ+LPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGE VD
Sbjct:  585 IESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEIEVTVEDGRDRHEVREGIDRVLGAIPGITTMVGQPIEHRLSHVLSGTPAAITINVYGEELSELRRVAKKVEGELQALPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGERVD 754
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BLAST of mRNA_M-pyrifera_M_contig125577.5174.1 vs. uniprot
Match: A0A2E2N5J9_9BACT (CusA/CzcA family heavy metal efflux RND transporter n=1 Tax=Phycisphaerae bacterium TaxID=2026778 RepID=A0A2E2N5J9_9BACT)

HSP 1 Score: 287 bits (735), Expect = 4.440e-88
Identity = 149/170 (87.65%), Postives = 161/170 (94.71%), Query Frame = 1 
Query:    1 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 510
            VESDRLATEVE+QLVEI+GVR+VSRRTGRAERDEHAEPVSNSE+EV+V+AG  R EVR  ID +L SIPGITTM+GQPIEHRLSHVLSGTPAA+AINVFGEDLSELRRVAKAIEGEL  LPGARDVNANREV+ITSLPIRYRHAELAAVGL+PADAAEQVREA+YGEVVD
Sbjct:  583 VESDRLATEVERQLVEIEGVRAVSRRTGRAERDEHAEPVSNSEIEVSVEAGHDRAEVRAAIDGVLGSIPGITTMIGQPIEHRLSHVLSGTPAAIAINVFGEDLSELRRVAKAIEGELSELPGARDVNANREVLITSLPIRYRHAELAAVGLTPADAAEQVREAVYGEVVD 752
[Close]
BLAST of mRNA_M-pyrifera_M_contig125577.5174.1 vs. uniprot
Match: A0A3M2CQK3_9BACT (Efflux RND transporter permease subunit n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3M2CQK3_9BACT)

HSP 1 Score: 282 bits (721), Expect = 5.110e-86
Identity = 144/170 (84.71%), Postives = 163/170 (95.88%), Query Frame = 1 
Query:    1 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 510
            VESDRLATEVEKQLV I GVRSVSRRTGRAERDEHAEPVS+SE+EVTVD G+ R +VR+ IDR+LAS+PGITTMVGQPIEHRLSH++SGTPAA+AI+++GEDL+ELRRVAK+IEGEL++LPGARDVNANREVMITSLPIRYRH +LAAVGLSPADAAEQVREA+YGEVVD
Sbjct:  583 VESDRLATEVEKQLVNIPGVRSVSRRTGRAERDEHAEPVSSSEIEVTVDPGQDRRKVRNNIDRVLASVPGITTMVGQPIEHRLSHIMSGTPAAIAISIYGEDLAELRRVAKSIEGELRTLPGARDVNANREVMITSLPIRYRHPDLAAVGLSPADAAEQVREALYGEVVD 752
[Close]
BLAST of mRNA_M-pyrifera_M_contig125577.5174.1 vs. uniprot
Match: A0A2E1VUN3_9BACT (CusA/CzcA family heavy metal efflux RND transporter n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2E1VUN3_9BACT)

HSP 1 Score: 280 bits (717), Expect = 1.730e-85
Identity = 146/170 (85.88%), Postives = 159/170 (93.53%), Query Frame = 1 
Query:    1 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 510
            VESDRLATEVE QL++++GVRSVSRRTGRAERDEHAEPVSNSE+EVT++ G  R+ VRDEID+ILA++PGITT VGQPIEHRLSHVLSGTPAA+AINVFGEDLS LR VAK IE ELQ+LPGARDVNANREVMITSLPIRYRHAELAA GLSPADAAEQVREAIYGEVVD
Sbjct:  583 VESDRLATEVENQLIQVEGVRSVSRRTGRAERDEHAEPVSNSELEVTLEDGADRLVVRDEIDQILAAVPGITTTVGQPIEHRLSHVLSGTPAAIAINVFGEDLSRLRAVAKEIEAELQALPGARDVNANREVMITSLPIRYRHAELAAAGLSPADAAEQVREAIYGEVVD 752
[Close]
BLAST of mRNA_M-pyrifera_M_contig125577.5174.1 vs. uniprot
Match: A0A518CVW3_9BACT (Cobalt-zinc-cadmium resistance protein CzcA n=1 Tax=Planctomycetes bacterium Pla163 TaxID=2528008 RepID=A0A518CVW3_9BACT)

HSP 1 Score: 273 bits (699), Expect = 5.450e-83
Identity = 144/170 (84.71%), Postives = 158/170 (92.94%), Query Frame = 1 
Query:    1 VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDAGRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFGEDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVGLSPADAAEQVREAIYGEVVD 510
            VESDRLATEVEK+LV IDGVRSVSRRTGRAERDEHAEPVSNSE+EVTV+ GR R  VR +ID +LA++PGITTMVGQPIEHRLSHVLSGTPAA+AINVFGEDL+ELRRVAKAIE EL+ +PG RDVNANREVMITSLPI+YRHAELAAVGL+PADAAEQV  A+YGEVVD
Sbjct:  583 VESDRLATEVEKKLVLIDGVRSVSRRTGRAERDEHAEPVSNSEIEVTVELGRDRDAVRRDIDVVLAAVPGITTMVGQPIEHRLSHVLSGTPAAIAINVFGEDLAELRRVAKAIEVELKGMPGTRDVNANREVMITSLPIQYRHAELAAVGLTPADAAEQVGAALYGEVVD 752
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig125577.5174.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3D4UKK0_9BACT2.550e-10299.41CusA/CzcA family heavy metal efflux RND transporte... [more]
A0A2D9C3G8_9BACT3.850e-101100.00CusA/CzcA family heavy metal efflux RND transporte... [more]
A0A812XWB5_9DINO5.290e-97100.00HelA protein n=1 Tax=Symbiodinium sp. CCMP2592 Tax... [more]
A0A2E2N5R6_9BACT8.670e-9491.76CusA/CzcA family heavy metal efflux RND transporte... [more]
A0A6M8VXQ9_9BACT5.310e-9090.00Efflux RND transporter permease subunit n=2 Tax=Pl... [more]
A0A7X0H3Y7_9BACT6.290e-8988.82HME family heavy-metal exporter n=1 Tax=Algisphaer... [more]
A0A2E2N5J9_9BACT4.440e-8887.65CusA/CzcA family heavy metal efflux RND transporte... [more]
A0A3M2CQK3_9BACT5.110e-8684.71Efflux RND transporter permease subunit n=1 Tax=Pl... [more]
A0A2E1VUN3_9BACT1.730e-8585.88CusA/CzcA family heavy metal efflux RND transporte... [more]
A0A518CVW3_9BACT5.450e-8384.71Cobalt-zinc-cadmium resistance protein CzcA n=1 Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig125577contigM-pyrifera_M_contig125577:3..512 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score143.7
Seed ortholog evalue8.1e-32
Seed eggNOG ortholog349741.Amuc_1059
Preferred nameczcA
KEGG koko:K07239,ko:K07787
KEGG TC2.A.6.1,2.A.6.1.4
KEGG Pathwayko02020,map02020
Hectar predicted targeting categoryother localisation
EggNOG free text desc.AcrB/AcrD/AcrF family
EggNOG OGs2ITM5@203494,46USJ@74201,COG3696@1,COG3696@2
COG Functional cat.P
Best tax levelVerrucomicrobiae
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko02000
Exons1
Model size510
Cds size510
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815771.7233255-CDS-M-pyrifera_M_contig125577:2..5121622815771.7233255-CDS-M-pyrifera_M_contig125577:2..512Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125577 3..512 +
1692277561.936492-CDS-M-pyrifera_M_contig125577:2..5121692277561.936492-CDS-M-pyrifera_M_contig125577:2..512Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125577 3..512 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig125577.5174.1prot_M-pyrifera_M_contig125577.5174.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig125577 3..512 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig125577.5174.1

>prot_M-pyrifera_M_contig125577.5174.1 ID=prot_M-pyrifera_M_contig125577.5174.1|Name=mRNA_M-pyrifera_M_contig125577.5174.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=170bp
VESDRLATEVEKQLVEIDGVRSVSRRTGRAERDEHAEPVSNSEVEVTVDA
GRSRVEVRDEIDRILASIPGITTMVGQPIEHRLSHVLSGTPAAVAINVFG
EDLSELRRVAKAIEGELQSLPGARDVNANREVMITSLPIRYRHAELAAVG
LSPADAAEQVREAIYGEVVD
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mRNA from alignment at M-pyrifera_M_contig125577:3..512+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig125577.5174.1 ID=mRNA_M-pyrifera_M_contig125577.5174.1|Name=mRNA_M-pyrifera_M_contig125577.5174.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=510bp|location=Sequence derived from alignment at M-pyrifera_M_contig125577:3..512+ (Macrocystis pyrifera P11B4 male)
GTCGAAAGCGACCGGCTCGCGACCGAGGTCGAGAAGCAGCTCGTCGAGAT
CGATGGTGTTCGGTCGGTGTCACGCCGCACTGGACGAGCCGAGCGTGATG
AACACGCCGAACCGGTCAGTAACTCGGAGGTCGAGGTCACGGTCGATGCA
GGGCGTTCACGAGTAGAGGTGCGTGATGAGATCGACCGCATTCTCGCATC
AATCCCAGGCATCACGACCATGGTCGGCCAACCGATCGAGCACCGCCTGA
GCCATGTCCTCTCGGGAACTCCCGCCGCCGTCGCGATCAATGTATTCGGC
GAGGACCTATCAGAGCTTCGTCGTGTCGCCAAAGCCATAGAAGGCGAGTT
ACAATCGCTCCCGGGTGCCAGGGATGTGAATGCCAATCGTGAAGTCATGA
TTACTTCGCTCCCGATCCGCTACCGTCACGCAGAGTTAGCTGCAGTCGGT
CTTAGCCCGGCGGATGCCGCCGAGCAGGTCCGTGAAGCGATCTACGGAGA
GGTAGTCGAC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig125577:3..512+

>mRNA_M-pyrifera_M_contig125577.5174.1 ID=mRNA_M-pyrifera_M_contig125577.5174.1|Name=mRNA_M-pyrifera_M_contig125577.5174.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1020bp|location=Sequence derived from alignment at M-pyrifera_M_contig125577:3..512+ (Macrocystis pyrifera P11B4 male)
GTCGAAAGCGACCGGCTCGCGACCGAGGTCGAGAAGCAGCTCGTCGAGAT
CGATGGTGTTCGGTCGGTGTCACGCCGCACTGGACGAGCCGAGCGTGATG
AACACGCCGAACCGGTCAGTAACTCGGAGGTCGAGGTCACGGTCGATGCA
GGGCGTTCACGAGTAGAGGTGCGTGATGAGATCGACCGCATTCTCGCATC
AATCCCAGGCATCACGACCATGGTCGGCCAACCGATCGAGCACCGCCTGA
GCCATGTCCTCTCGGGAACTCCCGCCGCCGTCGCGATCAATGTATTCGGC
GAGGACCTATCAGAGCTTCGTCGTGTCGCCAAAGCCATAGAAGGCGAGTT
ACAATCGCTCCCGGGTGCCAGGGATGTGAATGCCAATCGTGAAGTCATGA
TTACTTCGCTCCCGATCCGCTACCGTCACGCAGAGTTAGCTGCAGTCGGT
CTTAGCCCGGCGGATGCCGCCGAGCAGGTCCGTGAAGCGATCTACGGAGA
GGTAGTCGACGTCGAAAGCGACCGGCTCGCGACCGAGGTCGAGAAGCAGC
TCGTCGAGATCGATGGTGTTCGGTCGGTGTCACGCCGCACTGGACGAGCC
GAGCGTGATGAACACGCCGAACCGGTCAGTAACTCGGAGGTCGAGGTCAC
GGTCGATGCAGGGCGTTCACGAGTAGAGGTGCGTGATGAGATCGACCGCA
TTCTCGCATCAATCCCAGGCATCACGACCATGGTCGGCCAACCGATCGAG
CACCGCCTGAGCCATGTCCTCTCGGGAACTCCCGCCGCCGTCGCGATCAA
TGTATTCGGCGAGGACCTATCAGAGCTTCGTCGTGTCGCCAAAGCCATAG
AAGGCGAGTTACAATCGCTCCCGGGTGCCAGGGATGTGAATGCCAATCGT
GAAGTCATGATTACTTCGCTCCCGATCCGCTACCGTCACGCAGAGTTAGC
TGCAGTCGGTCTTAGCCCGGCGGATGCCGCCGAGCAGGTCCGTGAAGCGA
TCTACGGAGAGGTAGTCGAC
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