mRNA_M-pyrifera_M_contig125488.5144.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig125488.5144.1
Unique NamemRNA_M-pyrifera_M_contig125488.5144.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig125488.5144.1 vs. uniprot
Match: A0A1I7THL0_9PELO (Mannosyltransferase n=1 Tax=Caenorhabditis tropicalis TaxID=1561998 RepID=A0A1I7THL0_9PELO)

HSP 1 Score: 148 bits (374), Expect = 1.150e-39
Identity = 77/150 (51.33%), Postives = 101/150 (67.33%), Query Frame = 1 
Query:    1 MRGLDWLVLLAVWAYVAAAPYNKVEESFNVQASHDLLFH-GRDVAAYDHLEFPGVVPRTFLGPLALLPATAPLRFLLVDWLQLPKLSLLYCTRAALGLLSVAAFAYFRAAAGQVFGKEVAAWLAVITCCQFHLLFYMSRGLGNVFALALV 447
            M G +WLV++    ++  AP  KVEESFN+QASHDLLFH   +++ YDH  FPGVVPRTFLGPL L   ++P+ F+   W  +PK+  L   RA LGL++  AF YF  +  + FG++VA +L +I C QFH +FYMSR L N FAL LV
Sbjct:    1 MEGTEWLVIIVSLVHIILAPSTKVEESFNIQASHDLLFHLPTNMSNYDHSTFPGVVPRTFLGPLFLAALSSPMSFIFRFW-AIPKVWQLILVRATLGLMNTMAFLYFARSVSRCFGRDVAMFLRIIMCTQFHYIFYMSRPLPNSFALILV 149
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BLAST of mRNA_M-pyrifera_M_contig125488.5144.1 vs. uniprot
Match: UPI001884DC51 (LOW QUALITY PROTEIN: probable Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase n=1 Tax=Pollicipes pollicipes TaxID=41117 RepID=UPI001884DC51)

HSP 1 Score: 144 bits (362), Expect = 3.320e-39
Identity = 74/146 (50.68%), Postives = 96/146 (65.75%), Query Frame = 1 
Query:   10 LDWLVLLAVWAYVAAAPYNKVEESFNVQASHDLLFHGRDVAAYDHLEFPGVVPRTFLGPLALLPATAPLRFLLVDWLQLPKLSLLYCTRAALGLLSVAAFAYFRAAAGQVFGKEVAAWLAVITCCQFHLLFYMSRGLGNVFALALV 447
            ++W+VL  V  ++A  PY KVEESFN+QA HD+L+H  D++ YDHLEFPGVVPRTFLGPL L   +APL       L   K ++    R  LG LSV AF  FR +   VFG++VA WL  +T  QFH +FY++R L N+ A+ LV
Sbjct:   11 IEWVVLATVAVHLALCPYTKVEESFNMQAVHDILYHSTDISQYDHLEFPGVVPRTFLGPLVLAALSAPLAA--AGPLVASKFAIQSVVRMMLGSLSVCAFYRFRQSVESVFGRQVATWLVCVTVTQFHFMFYLTRTLPNMMAMPLV 154
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BLAST of mRNA_M-pyrifera_M_contig125488.5144.1 vs. uniprot
Match: ALG12_CAEEL (Probable Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase n=1 Tax=Caenorhabditis elegans TaxID=6239 RepID=ALG12_CAEEL)

HSP 1 Score: 145 bits (366), Expect = 1.660e-38
Identity = 73/150 (48.67%), Postives = 102/150 (68.00%), Query Frame = 1 
Query:    1 MRGLDWLVLLAVWAYVAAAPYNKVEESFNVQASHDLLFH-GRDVAAYDHLEFPGVVPRTFLGPLALLPATAPLRFLLVDWLQLPKLSLLYCTRAALGLLSVAAFAYFRAAAGQVFGKEVAAWLAVITCCQFHLLFYMSRGLGNVFALALV 447
            M G +W+V++    ++  AP  KVEESFNVQA+HDL+FH   +++ YDH +FPGVVPRTF+GP++L   ++P+ F+   W  +PK+  L   RA LGL++  AF YF  +  + FG+E A +L +I C QFH +FYMSR L N FAL LV
Sbjct:    1 MEGTEWIVIIVTIIHIILAPGTKVEESFNVQATHDLMFHLPTNLSNYDHSQFPGVVPRTFIGPISLAILSSPMSFIFRFW-AIPKMWQLLLIRATLGLMNAMAFLYFARSVNRKFGRETAMYLRLIMCTQFHYIFYMSRPLPNTFALILV 149
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BLAST of mRNA_M-pyrifera_M_contig125488.5144.1 vs. uniprot
Match: A0A261A8A2_CAERE (Valine--tRNA ligase (Fragment) n=4 Tax=Caenorhabditis TaxID=6237 RepID=A0A261A8A2_CAERE)

HSP 1 Score: 147 bits (370), Expect = 4.830e-38
Identity = 74/150 (49.33%), Postives = 102/150 (68.00%), Query Frame = 1 
Query:    1 MRGLDWLVLLAVWAYVAAAPYNKVEESFNVQASHDLLFH-GRDVAAYDHLEFPGVVPRTFLGPLALLPATAPLRFLLVDWLQLPKLSLLYCTRAALGLLSVAAFAYFRAAAGQVFGKEVAAWLAVITCCQFHLLFYMSRGLGNVFALALV 447
            M G +W+V++    ++  AP  KVEESFNVQA+HDLLFH   +++ YDH +FPGVVPRTFLGP+ L   ++P+ F+   W  +PK+  L   R+ LGL++  AF YF  +  + FG++ A +L +ITC QFH +FYMSR L N FAL LV
Sbjct: 1004 MEGTEWIVIIVSLVHIILAPGTKVEESFNVQATHDLLFHLPTNLSNYDHSQFPGVVPRTFLGPIFLAALSSPMSFIFRFW-AIPKMWQLLLIRSTLGLMNTMAFLYFARSVSRCFGRDTAMFLRIITCTQFHYIFYMSRPLPNSFALVLV 1152
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BLAST of mRNA_M-pyrifera_M_contig125488.5144.1 vs. uniprot
Match: A0A423SNS8_PENVA (Mannosyltransferase n=1 Tax=Penaeus vannamei TaxID=6689 RepID=A0A423SNS8_PENVA)

HSP 1 Score: 135 bits (340), Expect = 2.970e-37
Identity = 70/145 (48.28%), Postives = 90/145 (62.07%), Query Frame = 1 
Query:   10 LDWLVLLAVWAYVAAAPYNKVEESFNVQASHDLLFHGRDVAAYDHLEFPGVVPRTFLGPLALLPATAPLRFLLVDWLQLPKLSLLYCTRAALGLLSVAAFAYFRAAAGQVFGKEVAAWLAVITCCQFHLLFYMSRGLGNVFALAL 444
            +DWLV      ++  APY KVEESFN+QA HDLL+HG  ++ YDHLEFPGVVPRTF+GPL +     P+   ++  L L K    Y  R  LGLL + A+  FR    + FG  V  W  +IT  QFH ++Y+SR L N FALA+
Sbjct:    1 MDWLVGCVALVHLVVAPYTKVEESFNLQACHDLLYHGIHLSQYDHLEFPGVVPRTFVGPLVVSAIAYPI-VAIIKLLGLSKFLAQYIVRGVLGLLVILAWRRFRKQLQESFGPSVTFWFTLITASQFHFMYYLSRPLPNTFALAI 144
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BLAST of mRNA_M-pyrifera_M_contig125488.5144.1 vs. uniprot
Match: A0A2P6NAG9_9EUKA (Mannosyltransferase n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6NAG9_9EUKA)

HSP 1 Score: 141 bits (355), Expect = 8.170e-37
Identity = 76/154 (49.35%), Postives = 96/154 (62.34%), Query Frame = 1 
Query:   13 DWLVLLAVWAYVAAAPYNKVEESFNVQASHDLLFHGRDVAAYDHLEFPGVVPRTFLGPLALLPATAPLRFLLVDWLQL--------PKLSLLYCTRAALGLLSVAAFAYFRAAAGQVFGKEVAAWLAVITCC-QFHLLFYMSRGLGNVFALALV 447
            D  V      Y+   PY KVEESFN+QA HD+L+HGRD+  YDHLEFPGVVPR+F+GPL +   + PL F+L  ++ L        PKL      RA L  L ++ +   R AA  ++GK  A W ++ITC  QFHLLFY+SR L N FALALV
Sbjct:   33 DMYVFFTAIFYMVLCPYTKVEESFNMQAMHDILYHGRDIDKYDHLEFPGVVPRSFIGPLIVTAISYPLHFMLQTYMSLLSFEFDFSPKLISQLIVRAILAALVLSGYFRLRRAAAALYGKNTALWFSIITCLTQFHLLFYVSRPLPNTFALALV 186
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BLAST of mRNA_M-pyrifera_M_contig125488.5144.1 vs. uniprot
Match: UPI000F68919C (probable Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase n=1 Tax=Penaeus vannamei TaxID=6689 RepID=UPI000F68919C)

HSP 1 Score: 135 bits (340), Expect = 1.670e-36
Identity = 70/145 (48.28%), Postives = 90/145 (62.07%), Query Frame = 1 
Query:   10 LDWLVLLAVWAYVAAAPYNKVEESFNVQASHDLLFHGRDVAAYDHLEFPGVVPRTFLGPLALLPATAPLRFLLVDWLQLPKLSLLYCTRAALGLLSVAAFAYFRAAAGQVFGKEVAAWLAVITCCQFHLLFYMSRGLGNVFALAL 444
            +DWLV      ++  APY KVEESFN+QA HDLL+HG  ++ YDHLEFPGVVPRTF+GPL +     P+   ++  L L K    Y  R  LGLL + A+  FR    + FG  V  W  +IT  QFH ++Y+SR L N FALA+
Sbjct:    1 MDWLVGCVALVHLVVAPYTKVEESFNLQACHDLLYHGIHLSQYDHLEFPGVVPRTFVGPLVVSAIAYPI-VAIIKLLGLSKFLAQYIVRGVLGLLVILAWRRFRKQLQESFGPSVTFWFTLITASQFHFMYYLSRPLPNTFALAI 144
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BLAST of mRNA_M-pyrifera_M_contig125488.5144.1 vs. uniprot
Match: UPI000C6D263C (dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase-like isoform X1 n=2 Tax=Centruroides sculpturatus TaxID=218467 RepID=UPI000C6D263C)

HSP 1 Score: 137 bits (346), Expect = 2.180e-36
Identity = 68/146 (46.58%), Postives = 95/146 (65.07%), Query Frame = 1 
Query:   10 LDWLVLLAVWAYVAAAPYNKVEESFNVQASHDLLFHGRDVAAYDHLEFPGVVPRTFLGPLALLPATAPLRFLLVDWLQLPKLSLLYCTRAALGLLSVAAFAYFRAAAGQVFGKEVAAWLAVITCCQFHLLFYMSRGLGNVFALALV 447
            ++W++++A   ++   PY KVEESFN+QA HDLL+HG +++ YDHLEFPGVVPRTFLGP+ +   + P    +V+   L K  + Y  RA LG   + AF  +R +  + FG  +  WL +IT  QFH +FY+SR L N FAL LV
Sbjct:    1 MEWILIIAAAIHLIICPYTKVEESFNLQAIHDLLYHGSNISQYDHLEFPGVVPRTFLGPIVISMFSVPF-VTIVNTFGLSKFIIQYIVRAILGSFVIFAFYLYRCSLEKQFGNVLTTWLVLITASQFHFMFYLSRTLPNTFALILV 145
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BLAST of mRNA_M-pyrifera_M_contig125488.5144.1 vs. uniprot
Match: A0A087U4V9_STEMI (Mannosyltransferase (Fragment) n=7 Tax=Stegodyphus TaxID=175340 RepID=A0A087U4V9_STEMI)

HSP 1 Score: 140 bits (352), Expect = 2.650e-36
Identity = 72/147 (48.98%), Postives = 99/147 (67.35%), Query Frame = 1 
Query:    7 GLDWLVLLAVWAYVAAAPYNKVEESFNVQASHDLLFHGRDVAAYDHLEFPGVVPRTFLGPLALLPATAPLRFLLVDW-LQLPKLSLLYCTRAALGLLSVAAFAYFRAAAGQVFGKEVAAWLAVITCCQFHLLFYMSRGLGNVFALAL 444
            G++ L+ + +  +V   PY KVEESFN+QA HDLL+HG +++ YDHLEFPGVVPRTF+GPL +   + P  FL +++ L + K  + Y  R  LG L +AAF  F+ A  + +G  V+ WL +IT  QFH++FYMSR L N FAL L
Sbjct:  112 GVECLLYIVMIGHVMLCPYTKVEESFNLQAIHDLLYHGSNISQYDHLEFPGVVPRTFIGPLTIAALSYP--FLYINYILDMGKFFMQYIVRIMLGTLVLAAFHKFKEAIAKQYGSMVSLWLQIITASQFHVMFYMSRPLPNTFALVL 256
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BLAST of mRNA_M-pyrifera_M_contig125488.5144.1 vs. uniprot
Match: A0A2H2IMF8_CAEJA (Valine--tRNA ligase n=1 Tax=Caenorhabditis japonica TaxID=281687 RepID=A0A2H2IMF8_CAEJA)

HSP 1 Score: 142 bits (357), Expect = 2.700e-36
Identity = 73/150 (48.67%), Postives = 101/150 (67.33%), Query Frame = 1 
Query:    1 MRGLDWLVLLAVWAYVAAAPYNKVEESFNVQASHDLLFH-GRDVAAYDHLEFPGVVPRTFLGPLALLPATAPLRFLLVDWLQLPKLSLLYCTRAALGLLSVAAFAYFRAAAGQVFGKEVAAWLAVITCCQFHLLFYMSRGLGNVFALALV 447
            M G +W+V++    ++  AP  KVEESFNVQA+HDLLFH   +++ YDH  FPGVVPRTF+GP+ L   ++P+ F+   W+ +PK+  L   RA LGL++  +F YF  A  + FG+E A +L +I C QFH +FYMSR L N FA+ LV
Sbjct:  903 MEGTEWIVVIVSLIHIILAPGTKVEESFNVQATHDLLFHLPTNLSNYDHSSFPGVVPRTFIGPIFLAVLSSPMSFIFRFWM-IPKMWQLLLIRATLGLMNTMSFLYFARAVSRRFGRESAMFLRIIMCTQFHYIFYMSRPLPNSFAMILV 1051
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig125488.5144.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1I7THL0_9PELO1.150e-3951.33Mannosyltransferase n=1 Tax=Caenorhabditis tropica... [more]
UPI001884DC513.320e-3950.68LOW QUALITY PROTEIN: probable Dol-P-Man:Man(7)GlcN... [more]
ALG12_CAEEL1.660e-3848.67Probable Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,... [more]
A0A261A8A2_CAERE4.830e-3849.33Valine--tRNA ligase (Fragment) n=4 Tax=Caenorhabdi... [more]
A0A423SNS8_PENVA2.970e-3748.28Mannosyltransferase n=1 Tax=Penaeus vannamei TaxID... [more]
A0A2P6NAG9_9EUKA8.170e-3749.35Mannosyltransferase n=1 Tax=Planoprotostelium fung... [more]
UPI000F68919C1.670e-3648.28probable Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,... [more]
UPI000C6D263C2.180e-3646.58dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosy... [more]
A0A087U4V9_STEMI2.650e-3648.98Mannosyltransferase (Fragment) n=7 Tax=Stegodyphus... [more]
A0A2H2IMF8_CAEJA2.700e-3648.67Valine--tRNA ligase n=1 Tax=Caenorhabditis japonic... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig125488contigM-pyrifera_M_contig125488:3..449 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeNematoda
Seed ortholog score148.7
Seed ortholog evalue2.2e-33
Seed eggNOG ortholog1561998.Csp11.Scaffold615.g6004.t1
KEGG rclassRC00055,RC00523
KEGG koko:K01873,ko:K03847
KEGG ReactionR03665,R06260
KEGG Pathwayko00510,ko00513,ko00970,ko01100,map00510,map00513,map00970,map01100
KEGG ModuleM00055,M00359,M00360
Hectar predicted targeting categorysignal peptide
GOsGO:0000009,GO:0000030,GO:0003674,GO:0003824,GO:0006464,GO:0006486,GO:0006487,GO:0006488,GO:0006490,GO:0006629,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009100,GO:0009101,GO:0009987,GO:0016740,GO:0016757,GO:0016758,GO:0019538,GO:0034645,GO:0036211,GO:0043170,GO:0043412,GO:0043413,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044260,GO:0044267,GO:0070085,GO:0071704,GO:0097502,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.Alg9-like mannosyltransferase family
EggNOG OGs1KXS9@119089,38BVA@33154,3BBS2@33208,3D041@33213,40FQ3@6231,40XTR@6236,KOG2516@1,KOG2516@2759
EC2.4.1.260,6.1.1.9
COG Functional cat.G
CAZyGT22
Best tax levelRhabditida
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01003,ko01007,ko03016
Exons1
Model size447
Cds size447
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815770.2799687-CDS-M-pyrifera_M_contig125488:2..4491622815770.2799687-CDS-M-pyrifera_M_contig125488:2..449Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125488 3..449 -
1692277561.2630336-CDS-M-pyrifera_M_contig125488:2..4491692277561.2630336-CDS-M-pyrifera_M_contig125488:2..449Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig125488 3..449 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig125488.5144.1prot_M-pyrifera_M_contig125488.5144.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig125488 3..449 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig125488.5144.1

>prot_M-pyrifera_M_contig125488.5144.1 ID=prot_M-pyrifera_M_contig125488.5144.1|Name=mRNA_M-pyrifera_M_contig125488.5144.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=149bp
MRGLDWLVLLAVWAYVAAAPYNKVEESFNVQASHDLLFHGRDVAAYDHLE
FPGVVPRTFLGPLALLPATAPLRFLLVDWLQLPKLSLLYCTRAALGLLSV
AAFAYFRAAAGQVFGKEVAAWLAVITCCQFHLLFYMSRGLGNVFALALV
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mRNA from alignment at M-pyrifera_M_contig125488:3..449-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig125488.5144.1 ID=mRNA_M-pyrifera_M_contig125488.5144.1|Name=mRNA_M-pyrifera_M_contig125488.5144.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=447bp|location=Sequence derived from alignment at M-pyrifera_M_contig125488:3..449- (Macrocystis pyrifera P11B4 male)
ATGAGAGGACTTGATTGGCTGGTGCTGCTGGCAGTGTGGGCATATGTGGC
GGCGGCTCCGTACAACAAGGTGGAGGAGAGCTTTAACGTGCAGGCCAGCC
ACGACCTCCTCTTCCACGGCCGCGATGTGGCGGCCTACGATCACCTGGAG
TTTCCTGGAGTGGTGCCGCGCACCTTCCTAGGCCCTTTAGCGCTGCTGCC
GGCCACCGCTCCATTGCGGTTCCTGCTGGTGGACTGGCTGCAGCTGCCCA
AGCTCTCGCTCCTCTACTGCACGCGCGCCGCCCTGGGCCTCCTCTCCGTG
GCAGCCTTTGCATACTTCCGCGCAGCTGCAGGCCAGGTCTTTGGCAAGGA
AGTGGCCGCCTGGCTGGCAGTCATCACTTGCTGCCAGTTCCACCTGCTCT
TCTACATGAGCCGCGGCCTGGGCAACGTCTTTGCCCTCGCGCTCGTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig125488:3..449-

>mRNA_M-pyrifera_M_contig125488.5144.1 ID=mRNA_M-pyrifera_M_contig125488.5144.1|Name=mRNA_M-pyrifera_M_contig125488.5144.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=894bp|location=Sequence derived from alignment at M-pyrifera_M_contig125488:3..449- (Macrocystis pyrifera P11B4 male)
ATGAGAGGACTTGATTGGCTGGTGCTGCTGGCAGTGTGGGCATATGTGGC
GGCGGCTCCGTACAACAAGGTGGAGGAGAGCTTTAACGTGCAGGCCAGCC
ACGACCTCCTCTTCCACGGCCGCGATGTGGCGGCCTACGATCACCTGGAG
TTTCCTGGAGTGGTGCCGCGCACCTTCCTAGGCCCTTTAGCGCTGCTGCC
GGCCACCGCTCCATTGCGGTTCCTGCTGGTGGACTGGCTGCAGCTGCCCA
AGCTCTCGCTCCTCTACTGCACGCGCGCCGCCCTGGGCCTCCTCTCCGTG
GCAGCCTTTGCATACTTCCGCGCAGCTGCAGGCCAGGTCTTTGGCAAGGA
AGTGGCCGCCTGGCTGGCAGTCATCACTTGCTGCCAGTTCCACCTGCTCT
TCTACATGAGCCGCGGCCTGGGCAACGTCTTTGCCCTCGCGCTCGTCATG
AGAGGACTTGATTGGCTGGTGCTGCTGGCAGTGTGGGCATATGTGGCGGC
GGCTCCGTACAACAAGGTGGAGGAGAGCTTTAACGTGCAGGCCAGCCACG
ACCTCCTCTTCCACGGCCGCGATGTGGCGGCCTACGATCACCTGGAGTTT
CCTGGAGTGGTGCCGCGCACCTTCCTAGGCCCTTTAGCGCTGCTGCCGGC
CACCGCTCCATTGCGGTTCCTGCTGGTGGACTGGCTGCAGCTGCCCAAGC
TCTCGCTCCTCTACTGCACGCGCGCCGCCCTGGGCCTCCTCTCCGTGGCA
GCCTTTGCATACTTCCGCGCAGCTGCAGGCCAGGTCTTTGGCAAGGAAGT
GGCCGCCTGGCTGGCAGTCATCACTTGCTGCCAGTTCCACCTGCTCTTCT
ACATGAGCCGCGGCCTGGGCAACGTCTTTGCCCTCGCGCTCGTC
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