mRNA_M-pyrifera_M_contig124905.5064.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig124905.5064.1
Unique NamemRNA_M-pyrifera_M_contig124905.5064.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig124905.5064.1 vs. uniprot
Match: A0A836CH64_9STRA (WD40-repeat-containing domain protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CH64_9STRA)

HSP 1 Score: 143 bits (361), Expect = 2.570e-37
Identity = 64/127 (50.39%), Postives = 88/127 (69.29%), Query Frame = 1 
Query:    4 GEATGPPLDVPRSTTARQLEALLHELLGARERVPYTFYVEKGELEGTLGAFSRQAGVSTESSVAVEYQPLSVFRVRPVVRCSETMPGHSAPVLHVSFSADGGILGGGGGDCQIRFWDPITCTAKHTC 384
            GE  GP +D+P  +T  Q+E ++++LLG  E +PY FY+   E+  +LG   ++ G STE+ + V YQPL++FRVRPV RC++TMPGH+  VLHVS+S DG  L  GGGD  +RFWD  TCT + TC
Sbjct:   60 GEGKGPQIDIPLGSTVEQMEQVVNKLLGNAEDMPYCFYIGDTEITDSLGGTVQELGTSTENVLTVLYQPLAIFRVRPVTRCTDTMPGHTEAVLHVSYSPDGSQLASGGGDMTVRFWDVNTCTPRFTC 186
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BLAST of mRNA_M-pyrifera_M_contig124905.5064.1 vs. uniprot
Match: D8M696_BLAHO (Uncharacterized protein n=1 Tax=Blastocystis hominis TaxID=12968 RepID=D8M696_BLAHO)

HSP 1 Score: 142 bits (359), Expect = 3.300e-37
Identity = 63/123 (51.22%), Postives = 88/123 (71.54%), Query Frame = 1 
Query:   16 GPPLDVPRSTTARQLEALLHELLGARERVPYTFYVEKGELEGTLGAFSRQAGVSTESSVAVEYQPLSVFRVRPVVRCSETMPGHSAPVLHVSFSADGGILGGGGGDCQIRFWDPITCTAKHTC 384
            GP +D+P ++T  QLE LL+ELL + E VP+ FY++K E+  ++    R+ G +TE  V +EY+PLSV+RV PV RC++++PGH+  +LHVSFS DG +LG GGGD  +RFWD  TCT   TC
Sbjct:   37 GPQIDIPSNSTPSQLEELLNELLESEETVPFAFYIDKSEISSSVNESLRELGKTTEGVVTIEYRPLSVYRVNPVTRCTDSIPGHTEAILHVSFSPDGLVLGTGGGDMTVRFWDVNTCTPIKTC 159
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BLAST of mRNA_M-pyrifera_M_contig124905.5064.1 vs. uniprot
Match: A0A196SFH0_BLAHN (Notchless family protein n=1 Tax=Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII) TaxID=478820 RepID=A0A196SFH0_BLAHN)

HSP 1 Score: 142 bits (357), Expect = 6.410e-37
Identity = 60/127 (47.24%), Postives = 90/127 (70.87%), Query Frame = 1 
Query:    4 GEATGPPLDVPRSTTARQLEALLHELLGARERVPYTFYVEKGELEGTLGAFSRQAGVSTESSVAVEYQPLSVFRVRPVVRCSETMPGHSAPVLHVSFSADGGILGGGGGDCQIRFWDPITCTAKHTC 384
            GE+ GP +D+P ++T  QLE LL++L+ + E VP+ FY++  E+   +G   R+ G S+E+   ++Y+PLS++RV PV RC++++PGH+  +LHVSFS DG +LG GGGD  +RFWD  TCT   TC
Sbjct:   33 GESQGPQIDIPSNSTPEQLEGLLNQLIESEEPVPFAFYIKDNEIATNVGETLRELGESSETVTVIQYKPLSIYRVNPVTRCTDSIPGHTEAILHVSFSPDGNVLGTGGGDMTVRFWDVHTCTPIKTC 159
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BLAST of mRNA_M-pyrifera_M_contig124905.5064.1 vs. uniprot
Match: UPI000711B7F4 (hypothetical protein n=1 Tax=Blastocystis sp. subtype 4 TaxID=944170 RepID=UPI000711B7F4)

HSP 1 Score: 141 bits (355), Expect = 1.370e-36
Identity = 60/128 (46.88%), Postives = 89/128 (69.53%), Query Frame = 1 
Query:    1 RGEATGPPLDVPRSTTARQLEALLHELLGARERVPYTFYVEKGELEGTLGAFSRQAGVSTESSVAVEYQPLSVFRVRPVVRCSETMPGHSAPVLHVSFSADGGILGGGGGDCQIRFWDPITCTAKHTC 384
            +GE  GP +D+P  +T  QLE LL++L+ + E +P+ FY+ + E+  ++G   R+ G +TE    ++Y+PLSV+RV PV RC++++PGH+  +LHVSFS DG +LG GGGD  +RFWD  TCT   TC
Sbjct:   34 KGERKGPQIDIPSDSTPSQLEELLNQLIESEEPIPFAFYIGENEIAASVGETLREMGQTTEGVAVIQYRPLSVYRVNPVTRCTDSIPGHTEAILHVSFSPDGTVLGTGGGDMTVRFWDVSTCTPIKTC 161
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BLAST of mRNA_M-pyrifera_M_contig124905.5064.1 vs. uniprot
Match: A0A835XQD3_9CHLO (NLE domain-containing protein n=1 Tax=Edaphochlamys debaryana TaxID=47281 RepID=A0A835XQD3_9CHLO)

HSP 1 Score: 140 bits (354), Expect = 1.730e-36
Identity = 68/127 (53.54%), Postives = 86/127 (67.72%), Query Frame = 1 
Query:    4 GEATGPPLDVPRSTTARQLEALLHELLGARERVPYTFYVEKGELEGTLGAFSRQAGVSTESSVAVEYQPLSVFRVRPVVRCSETMPGHSAPVLHVSFSADGGILGGGGGDCQIRFWDPITCTAKHTC 384
            G+ TGP LD+P + T  QLE LL+ LL   E++PY+FY+E  EL G LGA  R AG+S E ++ V YQP +VFR+RPV RC+ +MPGHS  VL V+FS  G +L  G GD  +RFWD  T T KH C
Sbjct:   35 GDDTGPQLDIPHNVTPEQLETLLNGLLNNEEKLPYSFYIEDKELLGELGAHLRAAGLSVERALRVVYQPQAVFRIRPVARCTSSMPGHSEAVLSVNFSPCGTMLASGSGDTTVRFWDLNTQTPKHEC 161
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BLAST of mRNA_M-pyrifera_M_contig124905.5064.1 vs. uniprot
Match: A0A2J7ZSY8_9CHLO (Notchless n=1 Tax=Tetrabaena socialis TaxID=47790 RepID=A0A2J7ZSY8_9CHLO)

HSP 1 Score: 138 bits (348), Expect = 1.600e-35
Identity = 66/127 (51.97%), Postives = 86/127 (67.72%), Query Frame = 1 
Query:    4 GEATGPPLDVPRSTTARQLEALLHELLGARERVPYTFYVEKGELEGTLGAFSRQAGVSTESSVAVEYQPLSVFRVRPVVRCSETMPGHSAPVLHVSFSADGGILGGGGGDCQIRFWDPITCTAKHTC 384
            G+ATGP LD+P + T  QLE LL+ LL   E++PY+FY+E+ EL G LGA      +S E ++ + YQP +VFRVRPV RC+ ++PGHS  VL V+FS DG +L  G GD  +RFWD  T T KH C
Sbjct:   34 GDATGPQLDIPHNVTPMQLEVLLNGLLNNEEKLPYSFYIEEQELLGELGAHLGTHKLSVEKALRIVYQPQAVFRVRPVARCTSSIPGHSEAVLSVNFSPDGSMLASGSGDTTVRFWDLNTQTPKHEC 160
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BLAST of mRNA_M-pyrifera_M_contig124905.5064.1 vs. uniprot
Match: A0A482RLB7_9ARCH (NLE domain-containing protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482RLB7_9ARCH)

HSP 1 Score: 135 bits (339), Expect = 8.360e-35
Identity = 63/123 (51.22%), Postives = 83/123 (67.48%), Query Frame = 1 
Query:   16 GPPLDVPRSTTARQLEALLHELLGARERVPYTFYVEKGELEGTLGAFSRQAGVSTESSVAVEYQPLSVFRVRPVVRCSETMPGHSAPVLHVSFSADGGILGGGGGDCQIRFWDPITCTAKHTC 384
            GP L VP + T  QLEA+++ELL A E  PY+FY+ + E+  +L A     G STE    ++YQPL+VFRV PV RC+++MPGH+  +LHVSFS DG +   GGGD  +RFWD  TC  +HTC
Sbjct:  109 GPTLSVPLNITPAQLEAVVNELLEADEPTPYSFYLNEAEVLQSLKACMTAQGASTEEVQVIKYQPLAVFRVMPVSRCTDSMPGHTGAILHVSFSPDGRMCASGGGDATVRFWDVSTCMPRHTC 231
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BLAST of mRNA_M-pyrifera_M_contig124905.5064.1 vs. uniprot
Match: A0A7S1G396_9STRA (Hypothetical protein n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1G396_9STRA)

HSP 1 Score: 136 bits (343), Expect = 2.020e-34
Identity = 67/127 (52.76%), Postives = 85/127 (66.93%), Query Frame = 1 
Query:    4 GEATGPPLDVPRSTTARQLEALLHELLGARERVPYTFYVEKGELEGTLGAFSRQAGVSTESSVAVEYQPLSVFRVRPVVRCSETMPGHSAPVLHVSFSADGGILGGGGGDCQIRFWDPITCTAKHTC 384
            GEATGP LD+P  TT  Q+E L++ELL   ER PY F+V   EL   L        +STE  + ++YQPLS+F+V PV RC++T+PGH+  VLHVSFS DG +L  GGGD  +RFWD  T T K+TC
Sbjct:  149 GEATGPQLDIPLDTTQGQMEELVNELLENDERTPYAFFVNDEELLAKLRDAVEAQKLSTEVVLPIKYQPLSLFKVLPVARCTDTLPGHTDAVLHVSFSPDGLLLASGGGDATVRFWDVNTSTPKYTC 275
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BLAST of mRNA_M-pyrifera_M_contig124905.5064.1 vs. uniprot
Match: A0A482SD67_9ARCH (NLE domain-containing protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SD67_9ARCH)

HSP 1 Score: 134 bits (337), Expect = 1.110e-33
Identity = 70/131 (53.44%), Postives = 87/131 (66.41%), Query Frame = 1 
Query:    7 EATGPPLDVPRSTTARQLEALLHELLGARERVPYTFYVEKGE----LEGTLGAF-SRQAGVSTESSVAVEYQPLSVFRVRPVVRCSETMPGHSAPVLHVSFSADGGILGGGGGDCQIRFWDPITCTAKHTC 384
            EA GPPLDVP   TA+QLEAL++ LL   E +PY FYV   E    L+ TL A  + QA  S E ++ + YQPLSV++VRPV RC++TMPGH+  VLHVS+S  G  L  GGGD  +RFW+ IT    HTC
Sbjct:  105 EAAGPPLDVPSEATAKQLEALVNSLLQNEEVLPYAFYVNDMEVVENLKETLEALQASQATYSFEDTITIHYQPLSVYKVRPVTRCTDTMPGHTDSVLHVSYSPSGQRLASGGGDMTVRFWNVITNLPMHTC 235
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BLAST of mRNA_M-pyrifera_M_contig124905.5064.1 vs. uniprot
Match: A0A8J4BS91_9CHLO (NLE domain-containing protein n=1 Tax=Volvox africanus TaxID=51714 RepID=A0A8J4BS91_9CHLO)

HSP 1 Score: 130 bits (327), Expect = 1.610e-33
Identity = 62/127 (48.82%), Postives = 85/127 (66.93%), Query Frame = 1 
Query:    4 GEATGPPLDVPRSTTARQLEALLHELLGARERVPYTFYVEKGELEGTLGAFSRQAGVSTESSVAVEYQPLSVFRVRPVVRCSETMPGHSAPVLHVSFSADGGILGGGGGDCQIRFWDPITCTAKHTC 384
            G+ TGP LD+P + T  QLE LL+ LL   E++PY+F++E+ EL G LGA      +S E ++++ YQP +VFRVRPV RC+ ++PGHS  VL V+FS +G +L  G GD  +RFWD  T   KH C
Sbjct:   36 GDDTGPQLDIPHNVTPMQLEVLLNGLLNNEEKLPYSFFIEEQELLGELGAHLGIHKISVEKALSIVYQPQAVFRVRPVARCTASIPGHSEAVLSVNFSPNGSMLASGSGDTAVRFWDLGTQLPKHEC 162
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig124905.5064.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A836CH64_9STRA2.570e-3750.39WD40-repeat-containing domain protein n=1 Tax=Trib... [more]
D8M696_BLAHO3.300e-3751.22Uncharacterized protein n=1 Tax=Blastocystis homin... [more]
A0A196SFH0_BLAHN6.410e-3747.24Notchless family protein n=1 Tax=Blastocystis sp. ... [more]
UPI000711B7F41.370e-3646.88hypothetical protein n=1 Tax=Blastocystis sp. subt... [more]
A0A835XQD3_9CHLO1.730e-3653.54NLE domain-containing protein n=1 Tax=Edaphochlamy... [more]
A0A2J7ZSY8_9CHLO1.600e-3551.97Notchless n=1 Tax=Tetrabaena socialis TaxID=47790 ... [more]
A0A482RLB7_9ARCH8.360e-3551.22NLE domain-containing protein n=1 Tax=archaeon Tax... [more]
A0A7S1G396_9STRA2.020e-3452.76Hypothetical protein n=1 Tax=Bicosoecida sp. CB-20... [more]
A0A482SD67_9ARCH1.110e-3353.44NLE domain-containing protein n=1 Tax=archaeon Tax... [more]
A0A8J4BS91_9CHLO1.610e-3348.82NLE domain-containing protein n=1 Tax=Volvox afric... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig124905contigM-pyrifera_M_contig124905:3..509 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score131.7
Seed ortholog evalue3.2e-28
Seed eggNOG ortholog157072.XP_008866052.1
Preferred nameNLE1
KEGG koko:K07375,ko:K14855
KEGG Pathwayko04145,ko04540,ko05130,map04145,map04540,map05130
Hectar predicted targeting categoryother localisation
GOsGO:0000027,GO:0000151,GO:0001501,GO:0001655,GO:0001701,GO:0001756,GO:0001822,GO:0001824,GO:0001825,GO:0001826,GO:0003002,GO:0003674,GO:0003779,GO:0005102,GO:0005112,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005730,GO:0005737,GO:0005840,GO:0005856,GO:0005884,GO:0005938,GO:0006950,GO:0006970,GO:0006996,GO:0007010,GO:0007015,GO:0007154,GO:0007165,GO:0007166,GO:0007219,GO:0007275,GO:0007346,GO:0007389,GO:0008064,GO:0008092,GO:0008150,GO:0008154,GO:0009553,GO:0009628,GO:0009653,GO:0009790,GO:0009792,GO:0009887,GO:0009888,GO:0009892,GO:0009952,GO:0009966,GO:0009967,GO:0009968,GO:0009987,GO:0010466,GO:0010564,GO:0010605,GO:0010638,GO:0010639,GO:0010646,GO:0010647,GO:0010648,GO:0010941,GO:0010948,GO:0010951,GO:0015629,GO:0016043,GO:0019222,GO:0022411,GO:0022607,GO:0022613,GO:0022618,GO:0023051,GO:0023052,GO:0023056,GO:0023057,GO:0030029,GO:0030036,GO:0030042,GO:0030111,GO:0030154,GO:0030162,GO:0030177,GO:0030479,GO:0030832,GO:0030833,GO:0030834,GO:0030835,GO:0030836,GO:0030837,GO:0030863,GO:0030864,GO:0031323,GO:0031324,GO:0031333,GO:0031461,GO:0031974,GO:0031981,GO:0032268,GO:0032269,GO:0032271,GO:0032272,GO:0032465,GO:0032466,GO:0032501,GO:0032502,GO:0032535,GO:0032956,GO:0032970,GO:0032984,GO:0032991,GO:0033043,GO:0034622,GO:0035282,GO:0042254,GO:0042255,GO:0042273,GO:0042592,GO:0042641,GO:0042643,GO:0042981,GO:0043009,GO:0043066,GO:0043067,GO:0043069,GO:0043086,GO:0043154,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043242,GO:0043243,GO:0043244,GO:0043254,GO:0043281,GO:0043624,GO:0043933,GO:0044085,GO:0044087,GO:0044092,GO:0044422,GO:0044424,GO:0044428,GO:0044430,GO:0044444,GO:0044446,GO:0044448,GO:0044464,GO:0044877,GO:0045786,GO:0045861,GO:0045930,GO:0048229,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0048585,GO:0048646,GO:0048705,GO:0048731,GO:0048856,GO:0048869,GO:0048872,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051015,GO:0051016,GO:0051128,GO:0051129,GO:0051130,GO:0051171,GO:0051172,GO:0051246,GO:0051248,GO:0051261,GO:0051302,GO:0051336,GO:0051346,GO:0051493,GO:0051494,GO:0051495,GO:0051693,GO:0051716,GO:0051726,GO:0051782,GO:0052547,GO:0052548,GO:0060255,GO:0060429,GO:0060548,GO:0060828,GO:0061053,GO:0061484,GO:0061645,GO:0065003,GO:0065007,GO:0065008,GO:0065009,GO:0070013,GO:0070925,GO:0071826,GO:0071840,GO:0071944,GO:0072001,GO:0080008,GO:0080090,GO:0090066,GO:0090263,GO:0097435,GO:0099080,GO:0099081,GO:0099512,GO:0099513,GO:0099568,GO:0110053,GO:1901879,GO:1901880,GO:1901881,GO:1902494,GO:1902903,GO:1902904,GO:1902905,GO:1990234,GO:1990904,GO:2000116,GO:2000117,GO:2001233,GO:2001234,GO:2001267,GO:2001268
EggNOG free text desc.ribosomal large subunit assembly
EggNOG OGsKOG0271@1,KOG0271@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03009,ko03036,ko04147,ko04812
Exons1
Model size507
Cds size507
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815766.1951494-CDS-M-pyrifera_M_contig124905:2..5091622815766.1951494-CDS-M-pyrifera_M_contig124905:2..509Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig124905 3..509 +
1692277559.40849-CDS-M-pyrifera_M_contig124905:2..5091692277559.40849-CDS-M-pyrifera_M_contig124905:2..509Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig124905 3..509 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig124905.5064.1prot_M-pyrifera_M_contig124905.5064.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig124905 3..509 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig124905.5064.1

>prot_M-pyrifera_M_contig124905.5064.1 ID=prot_M-pyrifera_M_contig124905.5064.1|Name=mRNA_M-pyrifera_M_contig124905.5064.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=169bp
RGEATGPPLDVPRSTTARQLEALLHELLGARERVPYTFYVEKGELEGTLG
AFSRQAGVSTESSVAVEYQPLSVFRVRPVVRCSETMPGHSAPVLHVSFSA
DGGILGGGGGDCQIRFWDPITCTAKHTCSGHRSHVLATAFAPDMTRFASG
DRSGEIRIWSPKTGACLGK
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mRNA from alignment at M-pyrifera_M_contig124905:3..509+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig124905.5064.1 ID=mRNA_M-pyrifera_M_contig124905.5064.1|Name=mRNA_M-pyrifera_M_contig124905.5064.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=507bp|location=Sequence derived from alignment at M-pyrifera_M_contig124905:3..509+ (Macrocystis pyrifera P11B4 male)
CGTGGAGAGGCGACCGGCCCTCCTCTGGACGTTCCTCGCAGCACGACGGC
ACGGCAGCTGGAGGCGCTGCTGCACGAGCTGCTTGGGGCGCGGGAGCGCG
TGCCGTACACGTTCTACGTGGAGAAAGGGGAGCTGGAAGGGACGTTGGGC
GCGTTTTCGCGTCAGGCCGGCGTTTCCACCGAATCGAGCGTTGCAGTGGA
GTACCAGCCGCTGAGCGTGTTCCGAGTGCGTCCGGTGGTGCGGTGTTCCG
AGACAATGCCTGGCCACAGCGCTCCCGTGTTGCATGTGAGTTTTTCCGCC
GACGGTGGGATTCTGGGCGGCGGAGGTGGGGATTGCCAGATCCGGTTTTG
GGACCCCATCACTTGCACGGCCAAGCACACGTGTTCTGGGCACCGCTCGC
ACGTGCTGGCGACCGCCTTTGCGCCAGACATGACGCGGTTCGCTTCTGGA
GATCGCAGTGGGGAGATTCGAATTTGGAGTCCAAAAACAGGAGCTTGTCT
GGGGAAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig124905:3..509+

>mRNA_M-pyrifera_M_contig124905.5064.1 ID=mRNA_M-pyrifera_M_contig124905.5064.1|Name=mRNA_M-pyrifera_M_contig124905.5064.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1014bp|location=Sequence derived from alignment at M-pyrifera_M_contig124905:3..509+ (Macrocystis pyrifera P11B4 male)
CGTGGAGAGGCGACCGGCCCTCCTCTGGACGTTCCTCGCAGCACGACGGC
ACGGCAGCTGGAGGCGCTGCTGCACGAGCTGCTTGGGGCGCGGGAGCGCG
TGCCGTACACGTTCTACGTGGAGAAAGGGGAGCTGGAAGGGACGTTGGGC
GCGTTTTCGCGTCAGGCCGGCGTTTCCACCGAATCGAGCGTTGCAGTGGA
GTACCAGCCGCTGAGCGTGTTCCGAGTGCGTCCGGTGGTGCGGTGTTCCG
AGACAATGCCTGGCCACAGCGCTCCCGTGTTGCATGTGAGTTTTTCCGCC
GACGGTGGGATTCTGGGCGGCGGAGGTGGGGATTGCCAGATCCGGTTTTG
GGACCCCATCACTTGCACGGCCAAGCACACGTGTTCTGGGCACCGCTCGC
ACGTGCTGGCGACCGCCTTTGCGCCAGACATGACGCGGTTCGCTTCTGGA
GATCGCAGTGGGGAGATTCGAATTTGGAGTCCAAAAACAGGAGCTTGTCT
GGGGAAACGTGGAGAGGCGACCGGCCCTCCTCTGGACGTTCCTCGCAGCA
CGACGGCACGGCAGCTGGAGGCGCTGCTGCACGAGCTGCTTGGGGCGCGG
GAGCGCGTGCCGTACACGTTCTACGTGGAGAAAGGGGAGCTGGAAGGGAC
GTTGGGCGCGTTTTCGCGTCAGGCCGGCGTTTCCACCGAATCGAGCGTTG
CAGTGGAGTACCAGCCGCTGAGCGTGTTCCGAGTGCGTCCGGTGGTGCGG
TGTTCCGAGACAATGCCTGGCCACAGCGCTCCCGTGTTGCATGTGAGTTT
TTCCGCCGACGGTGGGATTCTGGGCGGCGGAGGTGGGGATTGCCAGATCC
GGTTTTGGGACCCCATCACTTGCACGGCCAAGCACACGTGTTCTGGGCAC
CGCTCGCACGTGCTGGCGACCGCCTTTGCGCCAGACATGACGCGGTTCGC
TTCTGGAGATCGCAGTGGGGAGATTCGAATTTGGAGTCCAAAAACAGGAG
CTTGTCTGGGGAAA
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