mRNA_M-pyrifera_M_contig124680.5023.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig124680.5023.1
Unique NamemRNA_M-pyrifera_M_contig124680.5023.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig124680.5023.1 vs. uniprot
Match: A0A7S1GA01_9STRA (alpha-1,2-Mannosidase n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1GA01_9STRA)

HSP 1 Score: 235 bits (599), Expect = 1.820e-72
Identity = 108/142 (76.06%), Postives = 121/142 (85.21%), Query Frame = 1 
Query:    7 RDLQNAKALAYTCYQMYARQPTGLSPEFVDFRAGQDLVVNGRAPFYMLRPEVSESLFILHQITGNPIYREWAWEIFSAIDKHCKTTYGHGHHPDVRDANRQPDDRMESFFLAETLKYLYLIQSPDHPISLDKFVFNTEAHPL 432
            RDL+NAKALAYTCYQMYAR  TGLS E+V+FR GQD V   RAPFY+LRPE SESLFILHQ+TGNPIYREW W +F AI+++ K  YG+G HPDVRD  R PDDRMESFFLAETLKYLYL+QSPDH +SL+ FVFNTEAHPL
Sbjct:  382 RDLKNAKALAYTCYQMYARMATGLSAEYVEFRGGQDFVAAPRAPFYILRPEASESLFILHQLTGNPIYREWGWRMFQAIERYTKVEYGYGAHPDVRDTARTPDDRMESFFLAETLKYLYLLQSPDHDVSLENFVFNTEAHPL 523
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BLAST of mRNA_M-pyrifera_M_contig124680.5023.1 vs. uniprot
Match: A0A5A8EAJ7_CAFRO (alpha-1,2-Mannosidase n=4 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8EAJ7_CAFRO)

HSP 1 Score: 242 bits (618), Expect = 5.890e-72
Identity = 105/149 (70.47%), Postives = 133/149 (89.26%), Query Frame = 1 
Query:    4 QRDLQNAKALAYTCYQMYARQPTGLSPEFVDFRAGQDLVVNGRAPFYMLRPEVSESLFILHQITGNPIYREWAWEIFSAIDKHCKTTYGHGHHPDVRDANRQPDDRMESFFLAETLKYLYLIQSPDHPISLDKFVFNTEAHPLRKFDQF 450
            QRDLQ AKA+AYTCYQMYARQ TG++PE+VDFR G D+VV  RAPFY+LRPE +ESL++LHQ+TGNPIYREW W+++ +ID++CKT +G+G  PDVRD +R  DDRMESFFLAET+KYLY++QSPDHPISL+++VFNTEAHP+R+FD +
Sbjct:  819 QRDLQAAKAIAYTCYQMYARQATGIAPEYVDFREGADIVVPSRAPFYILRPEAAESLYVLHQLTGNPIYREWGWKMWQSIDRYCKTKHGYGAFPDVRDVSRPADDRMESFFLAETMKYLYMLQSPDHPISLERYVFNTEAHPMRRFDHW 967
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BLAST of mRNA_M-pyrifera_M_contig124680.5023.1 vs. uniprot
Match: A0A5A8CLU8_CAFRO (alpha-1,2-Mannosidase n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CLU8_CAFRO)

HSP 1 Score: 242 bits (618), Expect = 2.260e-71
Identity = 105/149 (70.47%), Postives = 133/149 (89.26%), Query Frame = 1 
Query:    4 QRDLQNAKALAYTCYQMYARQPTGLSPEFVDFRAGQDLVVNGRAPFYMLRPEVSESLFILHQITGNPIYREWAWEIFSAIDKHCKTTYGHGHHPDVRDANRQPDDRMESFFLAETLKYLYLIQSPDHPISLDKFVFNTEAHPLRKFDQF 450
            QRDLQ AKA+AYTCYQMYARQ TG++PE+VDFR G D+VV  RAPFY+LRPE +ESL++LHQ+TGNPIYREW W+++ +ID++CKT +G+G  PDVRD +R  DDRMESFFLAET+KYLY++QSPDHPISL+++VFNTEAHP+R+FD +
Sbjct: 1209 QRDLQAAKAIAYTCYQMYARQATGIAPEYVDFREGADIVVPSRAPFYILRPEAAESLYVLHQLTGNPIYREWGWKMWQSIDRYCKTKHGYGAFPDVRDVSRPADDRMESFFLAETMKYLYMLQSPDHPISLERYVFNTEAHPMRRFDHW 1357
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BLAST of mRNA_M-pyrifera_M_contig124680.5023.1 vs. uniprot
Match: A0A7S0AP19_9STRA (alpha-1,2-Mannosidase n=2 Tax=Minutocellus polymorphus TaxID=265543 RepID=A0A7S0AP19_9STRA)

HSP 1 Score: 203 bits (516), Expect = 4.090e-64
Identity = 95/151 (62.91%), Postives = 117/151 (77.48%), Query Frame = 1 
Query:    1 AQRDLQNAKALAYTCYQMYARQPTGLSPEFVDFRAGQDLVVNGRAPFYMLRPEVSESLFILHQITGNPIYREWAWEIFSAIDKHCKTTYGHGHHPDVRDANRQPDDRMESFFLAETLKYLYLIQSPDHPIS-LDKFVFNTEAHPLRKFDQF 450
            AQRD+  AKA+ YTCYQMY+R  TG+SPE+VDF+ G D  V  RA +Y+LRPE  ES FILHQITG+PIYREW WE+F +I+++CKT   +G   DVR+ N+QP+DRMESFFLAET+KYLYL+Q PD  +  L + VFNTEAHP R FD F
Sbjct:   34 AQRDMATAKAITYTCYQMYSRMETGISPEYVDFK-GDDFEVPSRAFYYILRPEAVESFFILHQITGDPIYREWGWEVFQSIERYCKTKIAYGSLKDVRNTNQQPEDRMESFFLAETIKYLYLLQDPDTEVDVLKRHVFNTEAHPTRTFDHF 183
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BLAST of mRNA_M-pyrifera_M_contig124680.5023.1 vs. uniprot
Match: A0A7S3K687_9STRA (alpha-1,2-Mannosidase n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3K687_9STRA)

HSP 1 Score: 213 bits (542), Expect = 6.630e-62
Identity = 101/145 (69.66%), Postives = 115/145 (79.31%), Query Frame = 1 
Query:    1 AQRDLQNAKALAYTCYQMYARQPTGLSPEFVDFRAGQDLVVNGRAPFYMLRPEVSESLFILHQITGNPIYREWAWEIFSAIDKHCKTTYGHGHHPDVRDAN-RQPDDRMESFFLAETLKYLYLIQSPDHPISLDKFVFNTEAHPL 432
            AQRDL  AKALAYTCYQMY R  T L+PEF+ FRAG D +    APF +LRPE +ESLF+LH +TG+P+YREWAW IF A + HCKT  G+G HPDVRD+  R PDDRMESFFLAETLKYLYLIQ+PDH I L + VFNTEAHPL
Sbjct:  675 AQRDLDLAKALAYTCYQMYERTATKLAPEFITFRAGHDFIPAPSAPFNILRPETAESLFVLHHLTGDPMYREWAWNIFQAFESHCKTNLGYGAHPDVRDSKKRTPDDRMESFFLAETLKYLYLIQAPDHDIDLMRSVFNTEAHPL 819
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BLAST of mRNA_M-pyrifera_M_contig124680.5023.1 vs. uniprot
Match: A0A7S3XK61_HETAK (alpha-1,2-Mannosidase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XK61_HETAK)

HSP 1 Score: 207 bits (527), Expect = 5.320e-61
Identity = 96/147 (65.31%), Postives = 116/147 (78.91%), Query Frame = 1 
Query:    1 AQRDLQNAKALAYTCYQMYARQPTGLSPEFVDFRAGQDLVVNGRAPFYMLRPEVSESLFILHQITGNPIYREWAWEIFSAIDKHCKTTYGHGHHPDVRDANRQPDDRMESFFLAETLKYLYLIQSPDHPISLDKFVFNTEAHPLRKF 441
            A+RD+Q AK L +TCYQMY R  TGLSPEFV+FRAGQD+V    A FY+LRPE  E+LF L+  T +  YREW+WEIF AI+KHC+T+  +G HPDVR+  R+PDDRMESFFLAET KYLYLIQ PD PI L ++VFNTEAHP++ F
Sbjct:  470 ARRDMQVAKELGHTCYQMYRRTATGLSPEFVEFRAGQDIVNPSSASFYILRPEAVEALFYLYWFTKDHTYREWSWEIFQAIEKHCRTSIAYGAHPDVRNTGRKPDDRMESFFLAETFKYLYLIQDPDQPIDLYEYVFNTEAHPMKVF 616
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BLAST of mRNA_M-pyrifera_M_contig124680.5023.1 vs. uniprot
Match: B7S490_PHATC (alpha-1,2-Mannosidase (Fragment) n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7S490_PHATC)

HSP 1 Score: 201 bits (512), Expect = 6.650e-60
Identity = 96/146 (65.75%), Postives = 112/146 (76.71%), Query Frame = 1 
Query:    1 AQRDLQNAKALAYTCYQMYARQPTGLSPEFVDFRAGQDLVVNGRAPFYMLRPEVSESLFILHQITGNPIYREWAWEIFSAIDKHCKTTYGHGHHPDVRDANRQPDDRMESFFLAETLKYLYLIQSPDHPIS-LDKFVFNTEAHPLR 435
            AQRDLQ AKAL YTCYQMYAR  TG+S E+V F AG+D  +   AP Y+LRPE  ES FILHQ+TG+P+YREW WE+F AI+K+CKT YG+G   +V D N +P D MESFFLAETLKYLYL+Q PD  +  L K VFNTEAHPLR
Sbjct:  343 AQRDLQTAKALTYTCYQMYARMNTGISAEYVQFYAGRDFQIGRGAPHYLLRPEAVESFFILHQLTGDPVYREWGWEVFQAIEKYCKTPYGYGALNNVADVNGKPRDSMESFFLAETLKYLYLLQDPDTEVDVLHKHVFNTEAHPLR 488
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BLAST of mRNA_M-pyrifera_M_contig124680.5023.1 vs. uniprot
Match: A0A6U2WRQ7_9STRA (alpha-1,2-Mannosidase n=1 Tax=Amphiprora paludosa TaxID=265537 RepID=A0A6U2WRQ7_9STRA)

HSP 1 Score: 193 bits (491), Expect = 2.170e-58
Identity = 92/148 (62.16%), Postives = 113/148 (76.35%), Query Frame = 1 
Query:    1 AQRDLQNAKALAYTCYQMYARQPTGLSPEFVDFRAGQDLVVNGRAPFYMLRPEVSESLFILHQITGNPIYREWAWEIFSAIDKHCKTTYGHGHHPDVRDANRQPDDRMESFFLAETLKYLYLIQSPDHPIS-LDKFVFNTEAHPLRKF 441
            AQRDL+ A+A+ YTCYQMYAR  TG+S EFV F  G+D V+   AP Y+LRPE  ES FIL+Q+TG+P+YREW WE F AI+K+CKT   +G   +V+D N +P ++MESFFLAETLKYLYL+Q PD  I  L+K VFNTEAHPLR F
Sbjct:  172 AQRDLKTARAVTYTCYQMYARMNTGISAEFVQFEEGRDFVIGRGAPHYLLRPEAFESFFILNQLTGDPVYREWGWECFQAIEKYCKTHIAYGSLGNVQDTNGKPRNKMESFFLAETLKYLYLLQDPDTEIDILNKHVFNTEAHPLRMF 319
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BLAST of mRNA_M-pyrifera_M_contig124680.5023.1 vs. uniprot
Match: F0Y5N1_AURAN (Uncharacterized protein n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y5N1_AURAN)

HSP 1 Score: 205 bits (521), Expect = 2.520e-58
Identity = 95/145 (65.52%), Postives = 116/145 (80.00%), Query Frame = 1 
Query:    1 AQRDLQNAKALAYTCYQMYARQPTGLSPEFVDFRAGQDLVVNGRAPFYMLRPEVSESLFILHQITGNPIYREWAWEIFSAIDKHCKTTYGHGHHPDVRDANRQPDDRMESFFLAETLKYLYLIQSPDHPISLDKFVFNTEAHPLR 435
            A+RDL  A+AL +TC QMY RQPT L+PEFV FR G D+VV   APFY+LRPE +ESLF+LH++T NP+YR+W WEIFSAI+  C+T +G+G  PDVR   + PDDRMESFFLAETLKYLYL+Q P+H + L+  VFNTEAHPLR
Sbjct:  647 ARRDLHVAEALTHTCVQMYLRQPTKLAPEFVTFRRGADMVVAPSAPFYILRPETAESLFVLHEVTKNPVYRDWGWEIFSAIEARCRTAHGYGSVPDVRKVTK-PDDRMESFFLAETLKYLYLLQDPNHAVDLETAVFNTEAHPLR 790
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BLAST of mRNA_M-pyrifera_M_contig124680.5023.1 vs. uniprot
Match: A0A1E7FJU3_9STRA (alpha-1,2-Mannosidase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7FJU3_9STRA)

HSP 1 Score: 194 bits (492), Expect = 2.940e-57
Identity = 89/148 (60.14%), Postives = 111/148 (75.00%), Query Frame = 1 
Query:    1 AQRDLQNAKALAYTCYQMYARQPTGLSPEFVDFRAGQDLVVNGRAPFYMLRPEVSESLFILHQITGNPIYREWAWEIFSAIDKHCKTTYGHGHHPDVRDANRQPDDRMESFFLAETLKYLYLIQSPDHPISL-DKFVFNTEAHPLRKF 441
            AQRDL+ AKAL YTCYQMYAR PTG+SPE++ FR   D      AP Y+LRPE  ES F+L+ +TG+P+YREW WE+F AI+++CKT  G+G   DVR   R  +D+MESFFLAETLKY+YL+  P+ PI L +K VFNTEAHP+R F
Sbjct:  295 AQRDLKTAKALTYTCYQMYARMPTGISPEYIQFRKDNDFESGRGAPHYLLRPETVESFFVLYHLTGDPVYREWGWEVFQAIERYCKTDAGYGQLKDVRRVGRPAEDKMESFFLAETLKYIYLLFDPETPIDLLNKHVFNTEAHPVRTF 442
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig124680.5023.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S1GA01_9STRA1.820e-7276.06alpha-1,2-Mannosidase n=1 Tax=Bicosoecida sp. CB-2... [more]
A0A5A8EAJ7_CAFRO5.890e-7270.47alpha-1,2-Mannosidase n=4 Tax=Cafeteria roenbergen... [more]
A0A5A8CLU8_CAFRO2.260e-7170.47alpha-1,2-Mannosidase n=1 Tax=Cafeteria roenbergen... [more]
A0A7S0AP19_9STRA4.090e-6462.91alpha-1,2-Mannosidase n=2 Tax=Minutocellus polymor... [more]
A0A7S3K687_9STRA6.630e-6269.66alpha-1,2-Mannosidase n=1 Tax=Aureoumbra lagunensi... [more]
A0A7S3XK61_HETAK5.320e-6165.31alpha-1,2-Mannosidase n=1 Tax=Heterosigma akashiwo... [more]
B7S490_PHATC6.650e-6065.75alpha-1,2-Mannosidase (Fragment) n=2 Tax=Phaeodact... [more]
A0A6U2WRQ7_9STRA2.170e-5862.16alpha-1,2-Mannosidase n=1 Tax=Amphiprora paludosa ... [more]
F0Y5N1_AURAN2.520e-5865.52Uncharacterized protein n=1 Tax=Aureococcus anopha... [more]
A0A1E7FJU3_9STRA2.940e-5760.14alpha-1,2-Mannosidase n=1 Tax=Fragilariopsis cylin... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig124680contigM-pyrifera_M_contig124680:56..514 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score208.8
Seed ortholog evalue1.8e-51
Seed eggNOG ortholog44056.XP_009035587.1
Preferred nameMAN1A2
KEGG koko:K01230,ko:K03595
KEGG ReactionR05982,R06722
KEGG Pathwayko00510,ko00513,ko01100,ko04141,map00510,map00513,map01100,map04141
KEGG ModuleM00073,M00074
Hectar predicted targeting categoryother localisation
GOsGO:0000139,GO:0002252,GO:0002376,GO:0003674,GO:0003824,GO:0004553,GO:0004559,GO:0004571,GO:0005488,GO:0005509,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005768,GO:0005783,GO:0005793,GO:0005794,GO:0005802,GO:0005829,GO:0006139,GO:0006364,GO:0006396,GO:0006464,GO:0006486,GO:0006487,GO:0006491,GO:0006508,GO:0006511,GO:0006517,GO:0006725,GO:0006807,GO:0006950,GO:0007275,GO:0007568,GO:0007585,GO:0008150,GO:0008152,GO:0008340,GO:0009056,GO:0009057,GO:0009058,GO:0009059,GO:0009100,GO:0009101,GO:0009295,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0009987,GO:0010033,GO:0010243,GO:0010259,GO:0010467,GO:0010498,GO:0012505,GO:0015923,GO:0015924,GO:0016020,GO:0016021,GO:0016070,GO:0016072,GO:0016787,GO:0016798,GO:0019538,GO:0019941,GO:0022613,GO:0030163,GO:0030173,GO:0030323,GO:0030324,GO:0030433,GO:0031090,GO:0031224,GO:0031228,GO:0031300,GO:0031301,GO:0031410,GO:0031982,GO:0031984,GO:0032501,GO:0032502,GO:0033554,GO:0034470,GO:0034641,GO:0034645,GO:0034660,GO:0034976,GO:0035010,GO:0035295,GO:0036211,GO:0036503,GO:0036507,GO:0036508,GO:0042221,GO:0042254,GO:0042493,GO:0042644,GO:0042646,GO:0043161,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043412,GO:0043413,GO:0043632,GO:0044085,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044431,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0046483,GO:0046872,GO:0048286,GO:0048513,GO:0048731,GO:0048856,GO:0050896,GO:0051603,GO:0051716,GO:0060541,GO:0070085,GO:0071704,GO:0071840,GO:0072347,GO:0090304,GO:0097708,GO:0098588,GO:0098791,GO:1901135,GO:1901137,GO:1901360,GO:1901564,GO:1901565,GO:1901566,GO:1901575,GO:1901576,GO:1901698,GO:1904381
EggNOG free text desc.GTP binding
EggNOG OGsCOG1159@1,KOG1423@2759,KOG2204@1,KOG2204@2759
EC3.2.1.113
COG Functional cat.L
CAZyGH47
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03009,ko03029,ko04131
Exons1
Model size459
Cds size459
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815764.3404002-CDS-M-pyrifera_M_contig124680:55..5141622815764.3404002-CDS-M-pyrifera_M_contig124680:55..514Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig124680 56..514 -
1692277558.5759509-CDS-M-pyrifera_M_contig124680:55..5141692277558.5759509-CDS-M-pyrifera_M_contig124680:55..514Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig124680 56..514 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig124680.5023.1prot_M-pyrifera_M_contig124680.5023.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig124680 56..514 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig124680.5023.1

>prot_M-pyrifera_M_contig124680.5023.1 ID=prot_M-pyrifera_M_contig124680.5023.1|Name=mRNA_M-pyrifera_M_contig124680.5023.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=153bp
AQRDLQNAKALAYTCYQMYARQPTGLSPEFVDFRAGQDLVVNGRAPFYML
RPEVSESLFILHQITGNPIYREWAWEIFSAIDKHCKTTYGHGHHPDVRDA
NRQPDDRMESFFLAETLKYLYLIQSPDHPISLDKFVFNTEAHPLRKFDQF
PN*
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mRNA from alignment at M-pyrifera_M_contig124680:56..514-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig124680.5023.1 ID=mRNA_M-pyrifera_M_contig124680.5023.1|Name=mRNA_M-pyrifera_M_contig124680.5023.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=459bp|location=Sequence derived from alignment at M-pyrifera_M_contig124680:56..514- (Macrocystis pyrifera P11B4 male)
GCGCAGAGGGACCTGCAGAACGCCAAGGCGCTCGCGTACACGTGCTACCA
GATGTACGCCAGACAGCCCACCGGTCTCTCCCCCGAATTTGTCGATTTCC
GGGCGGGACAGGACCTGGTGGTCAACGGCAGGGCGCCCTTCTACATGCTA
AGGCCCGAAGTCTCAGAGTCGCTCTTCATCCTGCACCAGATCACGGGCAA
CCCCATCTACCGCGAGTGGGCCTGGGAGATCTTCTCCGCCATCGACAAGC
ACTGCAAGACCACCTACGGACACGGGCACCACCCAGACGTCAGGGACGCG
AACAGACAGCCGGACGACAGGATGGAGTCCTTCTTCCTCGCAGAAACCCT
CAAGTACCTCTACCTCATCCAGTCCCCAGACCACCCGATCTCGCTCGACA
AGTTCGTGTTCAACACCGAAGCACACCCCCTCAGGAAATTCGACCAGTTC
CCAAACTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig124680:56..514-

>mRNA_M-pyrifera_M_contig124680.5023.1 ID=mRNA_M-pyrifera_M_contig124680.5023.1|Name=mRNA_M-pyrifera_M_contig124680.5023.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=918bp|location=Sequence derived from alignment at M-pyrifera_M_contig124680:56..514- (Macrocystis pyrifera P11B4 male)
GCGCAGAGGGACCTGCAGAACGCCAAGGCGCTCGCGTACACGTGCTACCA
GATGTACGCCAGACAGCCCACCGGTCTCTCCCCCGAATTTGTCGATTTCC
GGGCGGGACAGGACCTGGTGGTCAACGGCAGGGCGCCCTTCTACATGCTA
AGGCCCGAAGTCTCAGAGTCGCTCTTCATCCTGCACCAGATCACGGGCAA
CCCCATCTACCGCGAGTGGGCCTGGGAGATCTTCTCCGCCATCGACAAGC
ACTGCAAGACCACCTACGGACACGGGCACCACCCAGACGTCAGGGACGCG
AACAGACAGCCGGACGACAGGATGGAGTCCTTCTTCCTCGCAGAAACCCT
CAAGTACCTCTACCTCATCCAGTCCCCAGACCACCCGATCTCGCTCGACA
AGTTCGTGTTCAACACCGAAGCACACCCCCTCAGGAAATTCGACCAGTTC
CCAAACTAGGCGCAGAGGGACCTGCAGAACGCCAAGGCGCTCGCGTACAC
GTGCTACCAGATGTACGCCAGACAGCCCACCGGTCTCTCCCCCGAATTTG
TCGATTTCCGGGCGGGACAGGACCTGGTGGTCAACGGCAGGGCGCCCTTC
TACATGCTAAGGCCCGAAGTCTCAGAGTCGCTCTTCATCCTGCACCAGAT
CACGGGCAACCCCATCTACCGCGAGTGGGCCTGGGAGATCTTCTCCGCCA
TCGACAAGCACTGCAAGACCACCTACGGACACGGGCACCACCCAGACGTC
AGGGACGCGAACAGACAGCCGGACGACAGGATGGAGTCCTTCTTCCTCGC
AGAAACCCTCAAGTACCTCTACCTCATCCAGTCCCCAGACCACCCGATCT
CGCTCGACAAGTTCGTGTTCAACACCGAAGCACACCCCCTCAGGAAATTC
GACCAGTTCCCAAACTAG
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