prot_M-pyrifera_M_contig8016.18787.1 (polypeptide) Macrocystis pyrifera P11B4 male

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NamemRNA_M-pyrifera_M_contig8016.18787.1
Unique Nameprot_M-pyrifera_M_contig8016.18787.1
Typepolypeptide
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Sequence length304
Homology
BLAST of mRNA_M-pyrifera_M_contig8016.18787.1 vs. uniprot
Match: D7FV84_ECTSI (Pseudouridine synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FV84_ECTSI)

HSP 1 Score: 403 bits (1035), Expect = 1.630e-137
Identity = 210/319 (65.83%), Postives = 245/319 (76.80%), Query Frame = 0
Query:    1 SFKGKAKVGTVPSMEKVYHALIAGTVTPDEKGVHQLREPLHFSRKLDGPGGDWTKPAEARVLLTKTYVSGQSVPWRFKPGTTATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRVSVGPIVLGDLKEGFSRELSRREVEALYKRCLPIDPLCPTVAEVQETLTSRLTQTARRLREASELAG--CQSGKISGAGEVEETGAGVAVVGDNNSARVCTENS--------RTHVGVGMGDV------EGLESIVRTLIDFARRDLHETPPGPDGESAGPKYCPGEEESLAQARAVPPNEAHMTDGYRCSRIEKLGLKYSSM 303
            ++KGKA VGTVPSMEKVYH L+AGT+TP++KG+  L+EPLHFSRKLDGPGG WTKPAEARVLLTKT+ +GQ VPWRFKPGT ATITWIEVVLREGKNRQIRRLCRRSEFELTRLHR+SVGPIVLGD++EG  REL+R+EVEALYKRCLP DPLCPT+  VQ+ L SRL+QTARR+R  +E+ G   ++ + S A +V +         ++   R   +          RT  G G   V      E L SI++ LIDFARRDLHETP GPDGESAGP YCPGE+ESLAQARAVPPNE HM  GYR S+IE+LGL Y +M
Sbjct:   69 TYKGKAAVGTVPSMEKVYHLLVAGTITPEDKGMDLLQEPLHFSRKLDGPGGSWTKPAEARVLLTKTFKTGQPVPWRFKPGTVATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRISVGPIVLGDVEEGSCRELTRQEVEALYKRCLPEDPLCPTIDAVQDILQSRLSQTARRIRTTAEVGGHRFKAREHSSAMDVPQKDMPERPTAEDAEQRRAAKARDGERGCPCRTTQGPGRRRVKAFATEEDLASIMKRLIDFARRDLHETPAGPDGESAGPDYCPGEKESLAQARAVPPNETHMVCGYRSSQIERLGLTYGTM 387          
BLAST of mRNA_M-pyrifera_M_contig8016.18787.1 vs. uniprot
Match: A0A6H5L3F2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L3F2_9PHAE)

HSP 1 Score: 319 bits (818), Expect = 4.110e-106
Identity = 171/269 (63.57%), Postives = 195/269 (72.49%), Query Frame = 0
Query:   51 GDWTKPAEARVLLTKTYVSGQSVPWRFKPGTTATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRVSVGPIVLGDLKEGFSRELSRREVEALYKRCLPIDPLCPTVAEVQETLTSRLTQTARRLREASELAG--CQSGKISGAGEVEETGAGVAVVGDNNSARVCTENSRTHVGV--------------GMGDVEGLESIVRTLIDFARRDLHETPPGPDGESAGPKYCPGEEESLAQARAVPPNEAHMTDGYRCSRIEKLGLKYSSM 303
            G WTKPAEARVLLTKT+ +GQ VPWRFKPGT ATITWIEVVLREGKNRQIRRLCRRSEFELTRLHR+SVGPIVLGD+ EG  REL+RREVEALYKRCLP DPLCPT+  VQ+ L SRL QTARR+R A+E+ G   ++ + S A +V +  A      ++   R   +      G                    E L SIV+ LIDF+RRDLHETP GPDGESAGP YCPGE+ESLAQARAVPPNE HM  GYR S+IE+LGL Y +M
Sbjct:   15 GSWTKPAEARVLLTKTFKTGQPVPWRFKPGTVATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRISVGPIVLGDVAEGSCRELTRREVEALYKRCLPEDPLCPTIDAVQDILKSRLAQTARRIRTAAEVGGHRFEAREHSSAMDVPQKDAPERPTREDAEQRWAAKVRDGECGCPXXXXXXXXXXRAKAFAGEEDLASIVKRLIDFSRRDLHETPAGPDGESAGPDYCPGEKESLAQARAVPPNETHMVCGYRSSQIERLGLTYGTM 283          
BLAST of mRNA_M-pyrifera_M_contig8016.18787.1 vs. uniprot
Match: A0A838IEP5_9DELT (Pseudouridine synthase n=1 Tax=Bradymonadaceae bacterium TaxID=2740543 RepID=A0A838IEP5_9DELT)

HSP 1 Score: 84.0 bits (206), Expect = 1.220e-15
Identity = 53/140 (37.86%), Postives = 75/140 (53.57%), Query Frame = 0
Query:   14 MEKVYHALIAGTVTPDEKGVHQLREPLHFSRKLDGPGGDWTKPAEARVLLTKTYVSGQSVPWRFKPGTTATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRVSVGPIVLGDLKEGFSRELSRREVEALYKRCLPIDPL 153
            +EKVYH  I G V   +  + +L EPL       GPG   T+PA ARV+  +   S                 WIE+V+ EG++RQ+R+LC RS  ++ +L R+ +GP+ LG+L+  + R L R EV ALY   L  D L
Sbjct:   68 VEKVYHVKIKGIVEDGDARLARLEEPLK-----SGPGKT-TEPARARVVERRDKTS-----------------WIELVICEGRHRQVRKLCERSGLQIRKLRRMRLGPLELGELRLRWCRPLERAEVAALYAAALADDAL 184          
BLAST of mRNA_M-pyrifera_M_contig8016.18787.1 vs. uniprot
Match: A0A525VTS7_9BACT (Pseudouridine synthase n=2 Tax=unclassified Nitrospira TaxID=2652172 RepID=A0A525VTS7_9BACT)

HSP 1 Score: 82.8 bits (203), Expect = 2.910e-15
Identity = 49/117 (41.88%), Postives = 67/117 (57.26%), Query Frame = 0
Query:   28 PDEKGVHQLREPLHFSRKLDGPGGDWTKPAEARVLLTKTYVSGQSVPWRFKPGTTATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRVSVGPIVLGDLKEGFSRELSRREVEALY 144
            P+E+ + QLR  +  S K        T+PAE R+L     +S +SVP RF+        W+E+ LREG NRQ+RR+         RL RV+VGPIVLG L+ G  REL+  E+E +Y
Sbjct:   82 PNEEALEQLRAGVVLSGKK-------TRPAEVRLLANHPQLSDRSVPIRFRKNVPTA--WVEMTLREGMNRQVRRMTASVGHPTLRLVRVAVGPIVLGGLQAGEWRELTSGEIEQIY 189          
BLAST of mRNA_M-pyrifera_M_contig8016.18787.1 vs. uniprot
Match: A0A532DIV7_9BACT (Pseudouridine synthase n=2 Tax=Nitrospira sp. TaxID=70125 RepID=A0A532DIV7_9BACT)

HSP 1 Score: 79.0 bits (193), Expect = 6.330e-14
Identity = 50/131 (38.17%), Postives = 72/131 (54.96%), Query Frame = 0
Query:   14 MEKVYHALIAGTVTPDEKGVHQLREPLHFSRKLDGPGGDWTKPAEARVLLTKTYVSGQSVPWRFKPGTTATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRVSVGPIVLGDLKEGFSRELSRREVEALY 144
            + KVY  L+     P E+ + QLRE +  + K+       TKPAE R+L     +  + VP RF+        W+E+ LREG NRQ+RR+         RL RV+VGPIVLG ++ G  R+L+  E+E +Y
Sbjct:   70 LAKVY--LVQIERVPKEEVLRQLREGVMLNGKI-------TKPAEVRLLPDDPQLPDRPVPIRFRKNVPTA--WVEMTLREGLNRQVRRMTASVGHPTLRLVRVAVGPIVLGGIQPGEWRDLTSSEIEQIY 189          
BLAST of mRNA_M-pyrifera_M_contig8016.18787.1 vs. uniprot
Match: A0A7V2V0T7_9BACT (Pseudouridine synthase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A7V2V0T7_9BACT)

HSP 1 Score: 80.9 bits (198), Expect = 6.510e-14
Identity = 52/141 (36.88%), Postives = 74/141 (52.48%), Query Frame = 0
Query:    4 GKAKVGTVPSMEKVYHALIAGTVTPDEKGVHQLREPLHFSRKLDGPGGDW-TKPAEARVLLTKTYVSGQSVPWRFKPGTTATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRVSVGPIVLGDLKEGFSRELSRREVEAL 143
            G    G    + K Y A + G +T  ++G+ +LR+ +         G  W T+PAE R+L             R   GTT    W+E+ LREG NRQ+RR+C+    E+ +L R+ VGP+ LG L  G  R L+RRE+EAL
Sbjct:  157 GALIAGVDSPVPKTYEAKVRGVMT--DEGIARLRQGVTL-------GDGWRTRPAEVRLL-------------RANEGTT----WLEITLREGMNRQVRRMCQAVGHEVIKLRRIRVGPVELGGLPSGAVRPLTRREIEAL 271          
BLAST of mRNA_M-pyrifera_M_contig8016.18787.1 vs. uniprot
Match: A0A533ZMC8_9BACT (Pseudouridine synthase n=5 Tax=unclassified Nitrospirae TaxID=1298915 RepID=A0A533ZMC8_9BACT)

HSP 1 Score: 79.0 bits (193), Expect = 1.100e-13
Identity = 45/132 (34.09%), Postives = 73/132 (55.30%), Query Frame = 0
Query:   14 MEKVYHALIAGTVTPDEKGVHQLREPLHFSRKLDGPGGDWTKPAEARVLLTKTYVSGQSVPWRFKPGTTATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRVSVGPIVLGDLKEGFSRELSRREVEALYK 145
            + K+Y A +     P+++ + QLR  +    K        T+PAE R+L     +  + VP RF+        W+E+ LREG NRQ+RR+    E    RL R+++GPI+LGDL+ G  R+L+  E++ +Y+
Sbjct:   81 LPKIYLAQVERI--PNDEALAQLRAGILLKGKR-------TRPAEVRLLSDAPELPDRPVPIRFRKNVPTA--WLEITLREGLNRQVRRMTAAVEHPTVRLVRIAIGPIMLGDLQPGHWRDLANSEIDQIYR 201          
BLAST of mRNA_M-pyrifera_M_contig8016.18787.1 vs. uniprot
Match: A0A1W9IVN7_9BACT (Pseudouridine synthase n=1 Tax=Nitrospira sp. ST-bin5 TaxID=1978765 RepID=A0A1W9IVN7_9BACT)

HSP 1 Score: 78.2 bits (191), Expect = 1.980e-13
Identity = 51/130 (39.23%), Postives = 72/130 (55.38%), Query Frame = 0
Query:   14 MEKVYHALIAGTVTPDEKGVHQLREPLHFSRKLDGPGGDWTKPAEARVLLTKTYVSGQSVPWRFKPGTTATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRVSVGPIVLGDLKEGFSRELSRREVEAL 143
            + KVY  L+     PDE+ + QLR+ +  SR         T+PAE R+L     +  + VP RF+   T    WIE+ +REG NRQ+RR+         RL RV++GPI LG+L  G  REL+  EV+A+
Sbjct:   77 LPKVY--LVQVERIPDEQALRQLRQGVALSRTK-------TRPAEVRLLDEPPSLPDRPVPIRFRK--TVPTCWIEMTIREGMNRQVRRMTAAVGHPTLRLIRVAIGPIPLGNLAPGAWRELTAGEVQAI 195          
BLAST of mRNA_M-pyrifera_M_contig8016.18787.1 vs. uniprot
Match: E6SKM7_THEM7 (Pseudouridine synthase n=1 Tax=Thermaerobacter marianensis (strain ATCC 700841 / DSM 12885 / JCM 10246 / 7p75a) TaxID=644966 RepID=E6SKM7_THEM7)

HSP 1 Score: 79.3 bits (194), Expect = 3.020e-13
Identity = 60/157 (38.22%), Postives = 82/157 (52.23%), Query Frame = 0
Query:   14 MEKVYHALIAGTVTPDEKGVHQLREPLHFSRKLDGPGGDWTKPAEARVLLTKTYVSGQSVPWRFKPGTTATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRVSVGPIVLGDLKEGFSRELSRREVEALYKRC-LPI--DPLCPTVAEVQETLTSR 167
            ++KVYH L+ G + P    + +LR+ +      DGP     +   AR+L             R +PG      W+EVVLREG+ RQ+RR+CR   +++ RL RV+VGP+ LG L  G  R LSR+EVEAL     LPI  D   P        +TSR
Sbjct:  136 VDKVYHVLVRGPLEPQR--LRRLRDGVLLD---DGPA----RARSARIL-------------RPRPGGG----WVEVVLREGRKRQVRRMCRAVGWDVVRLVRVAVGPLRLGRLTAGQWRPLSRQEVEALRAAAGLPIHGDEPAPREGGAAPAITSR 266          
BLAST of mRNA_M-pyrifera_M_contig8016.18787.1 vs. uniprot
Match: A0A533XS16_9BACT (Pseudouridine synthase n=1 Tax=Nitrospirae bacterium TaxID=2026887 RepID=A0A533XS16_9BACT)

HSP 1 Score: 77.0 bits (188), Expect = 3.510e-13
Identity = 47/127 (37.01%), Postives = 69/127 (54.33%), Query Frame = 0
Query:   14 MEKVYHALIAGTVTPDEKGVHQLREPLHFSRKLDGPGGDWTKPAEARVLLTKTYVSGQSVPWRFKPGTTATITWIEVVLREGKNRQIRRLCRRSEFELTRLHRVSVGPIVLGDLKEGFSRELSRREV 140
            + KVY A +     P+EK + QLR+ +         GG  T+P E  +L     +  + VP RF+        W+E++L EG NRQ+RR+         RL RV++GP+VLGDL+ G  REL+ RE+
Sbjct:   74 LPKVYLAQVERI--PNEKALEQLRKGVVL-------GGKKTRPVEVGLLPDHPQLPDRPVPIRFRKNVVTA--WLEIILHEGLNRQVRRMTATVGHPTLRLVRVAIGPVVLGDLQPGHWRELTSREI 189          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig8016.18787.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FV84_ECTSI1.630e-13765.83Pseudouridine synthase n=1 Tax=Ectocarpus siliculo... [more]
A0A6H5L3F2_9PHAE4.110e-10663.57Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A838IEP5_9DELT1.220e-1537.86Pseudouridine synthase n=1 Tax=Bradymonadaceae bac... [more]
A0A525VTS7_9BACT2.910e-1541.88Pseudouridine synthase n=2 Tax=unclassified Nitros... [more]
A0A532DIV7_9BACT6.330e-1438.17Pseudouridine synthase n=2 Tax=Nitrospira sp. TaxI... [more]
A0A7V2V0T7_9BACT6.510e-1436.88Pseudouridine synthase n=1 Tax=Planctomycetes bact... [more]
A0A533ZMC8_9BACT1.100e-1334.09Pseudouridine synthase n=5 Tax=unclassified Nitros... [more]
A0A1W9IVN7_9BACT1.980e-1339.23Pseudouridine synthase n=1 Tax=Nitrospira sp. ST-b... [more]
E6SKM7_THEM73.020e-1338.22Pseudouridine synthase n=1 Tax=Thermaerobacter mar... [more]
A0A533XS16_9BACT3.510e-1337.01Pseudouridine synthase n=1 Tax=Nitrospirae bacteri... [more]

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InterPro
Analysis Name: InterProScan on OGS1.0 of Macrocystis pyrifera male
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.2350.10coord: 8..149
e-value: 3.9E-18
score: 67.5
NoneNo IPR availablePANTHERPTHR21600:SF23RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE Ecoord: 14..144
NoneNo IPR availablePANTHERPTHR21600RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE Bcoord: 14..144
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 13..141

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig8016contigM-pyrifera_M_contig8016:5991..10076 -
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.0 of Macrocystis pyrifera male2022-09-29
Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-16
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig8016.18787.1mRNA_M-pyrifera_M_contig8016.18787.1Macrocystis pyrifera P11B4 malemRNAM-pyrifera_M_contig8016 5991..10076 -


Sequences
The following sequences are available for this feature:

polypeptide sequence

>prot_M-pyrifera_M_contig8016.18787.1 ID=prot_M-pyrifera_M_contig8016.18787.1|Name=mRNA_M-pyrifera_M_contig8016.18787.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=304bp
SFKGKAKVGTVPSMEKVYHALIAGTVTPDEKGVHQLREPLHFSRKLDGPG
GDWTKPAEARVLLTKTYVSGQSVPWRFKPGTTATITWIEVVLREGKNRQI
RRLCRRSEFELTRLHRVSVGPIVLGDLKEGFSRELSRREVEALYKRCLPI
DPLCPTVAEVQETLTSRLTQTARRLREASELAGCQSGKISGAGEVEETGA
GVAVVGDNNSARVCTENSRTHVGVGMGDVEGLESIVRTLIDFARRDLHET
PPGPDGESAGPKYCPGEEESLAQARAVPPNEAHMTDGYRCSRIEKLGLKY
SSM*
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Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR020103PsdUridine_synth_cat_dom_sf