prot_M-pyrifera_M_contig10890.1869.1 (polypeptide) Macrocystis pyrifera P11B4 male

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview


Name	mRNA_M-pyrifera_M_contig10890.1869.1
Unique Name	prot_M-pyrifera_M_contig10890.1869.1
Type	polypeptide
Organism	Macrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Sequence length	479

Homology

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BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot
Match: A0A6H5JEX6_9PHAE (PseudoU_synth_1 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JEX6_9PHAE)

HSP 1 Score: 328 bits (840), Expect = 8.180e-101
Identity = 257/480 (53.54%), Postives = 280/480 (58.33%), Query Frame = 0

Query:   45 ARVPPAISARGLSWKEKQRXXXXXXXXXXXXXXVAG--KGKKKRGQERDFDHSRWRKRTVAFQLMYEGGGYAGFCSQSEGNEDTIEKRLFHAFTTTKLVERIATGDNYSRCGRTDRGVSALGNVVTVSARSKFPKEL-DVELPTSPLGSVRVV---------SGGGXXXXXXXXXXXXRDEGSGSXXXXXXXXXXXXXXXXXXRTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDGREGMEREAR-----MGDVERSSGGG--GRGXXXXXXXXXXXXXXXXXXXXXSGGRECEASGQDASNGKATGEADKXXXXXXXXXXXXXKGDGTV---------------EVTEIDYCGVLNKVLPPDIRALAWAPVTEGFSARFSCSDRTYRQEKAYFFLARDLDIEAMDEAASRIVGHHDFRNLCKIDVANVSNFVRFIKFASVRPVPSPSP----DYGGT-------EYYRMCVFEV 479
            A VPP    RGLSW+EKQR     XXXXXXXXX     K KK  GQ+RDFDH+RWRKRTVA QLMYEGGGYAGFCSQSEGNEDTIEK LF A TTTKLVE IATGDNYSRCGRTDRGVSALGNV+TVS RSKFPK + D +LPTSPLGS+ V             G              EGS +                  +                                  G   EG   ++R       D   + GGG  GRG       XXXXXXXXXXXXXX                        XXXXXXXXXXXXX                     E+TEIDYCG+LN+VLPP IRALAWAPVTEGFSARFSCSDRTYR    YFF+ARD++I+AM+EAAS+IVG HDFRNLCKIDVANVSNFVR IK ASVRPV SPSP    D+ GT       EYYR+CVFEV
Sbjct:   62 APVPPQ-GPRGLSWREKQRQKAAEXXXXXXXXXXXXGRKSKKGAGQQRDFDHARWRKRTVAVQLMYEGGGYAGFCSQSEGNEDTIEKHLFRALTTTKLVESIATGDNYSRCGRTDRGVSALGNVITVSMRSKFPKHVPDADLPTSPLGSLWVAPIAPPQNAAKSVGENDGGFKRESQTMPEGSAAAEQEQPAAGGADEGSGLAK----------------------------------GKSSEGCPSDSRHPASGASDPCTAPGGGEVGRGEVASGNPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAGGEITEIDYCGILNRVLPPGIRALAWAPVTEGFSARFSCSDRTYR----YFFMARDMNIKAMNEAASKIVGSHDFRNLCKIDVANVSNFVRHIKHASVRPVQSPSPSNTSDHEGTARGEEEEEYYRVCVFEV 502

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BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot
Match: D7FHT8_ECTSI (Pseudouridylate synthase 3 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FHT8_ECTSI)

HSP 1 Score: 272 bits (695), Expect = 1.620e-82
Identity = 226/421 (53.68%), Postives = 246/421 (58.43%), Query Frame = 0

Query:   45 ARVPPAISARGLSWKEKQRXXXXXXXXXXXXXXVAG----KGKKKRGQERDFDHSRWRKRTVAFQLMYEGGGYAGFCSQSEGNEDTIEKRLFHAFTTTKLVERIATGDNYSRCGRTDRGVSALGNVVTVSARSKFPKEL-DVELPTSPLGSVRVVSGG---------GXXXXXXXXXXXXRDEGSGSXXXXXXXXXXXXXXXXXXRTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDGREGMEREARMGDVERSSGGGGR--GXXXXXXXXXXXXXXXXXXXXXSGGRECEASGQDASNGKATGEADKXXXXXXXXXXXXXKGDGTV----------EVTEIDYCGVLNKVLPPDIRALAWAPVTEGFSARFSCSDRTYRQEKAYFFLARDLDIEAMDEAASRIVGHHDFRNLCKI 439
            A VPP    RGLSW+EKQR     XXXXXXXXX       K KK  GQ+RDFDHSRWRKRTVA QLMYEGGGYAGFCSQSEGNE+TIEK LF A TTTKLVE I TGDNYSRCGRTDRGVSALGNV+TVS RSKFPK + D +LPTSPLGS+ V             G              EGS +                  +                                    G      EA  G+V R     G    XXXXXXXXXXXXXXXXXXXXX         G +   G        XXXXXXXXXXXXX                EVTEIDYCG+LN+VLPP +R LAWAPVTEGFSARFSCSDRTYR    YFF+ARD+D++AM+EAAS+IVG HDFRNLCKI
Sbjct:   47 APVPPQ-GPRGLSWREKQRQKAAEXXXXXXXXXXXXXXGRKSKKGAGQQRDFDHSRWRKRTVAVQLMYEGGGYAGFCSQSEGNEETIEKHLFRALTTTKLVETITTGDNYSRCGRTDRGVSALGNVITVSMRSKFPKHVPDADLPTSPLGSLWVAPTAPPPNGAESVGEHDGAPKKENQTMPEGSAAAEQEQPAAGVAEEGSGLAK--------------------GKNSEGCPSDSRHQASGAPEPWTEAGGGEVGRGEVASGNPPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLRGSEGERGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAGGEVTEIDYCGILNRVLPPGVRTLAWAPVTEGFSARFSCSDRTYR----YFFMARDMDVKAMNEAASKIVGSHDFRNLCKI 442

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BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot
Match: A0A7S3H897_9STRA (Hypothetical protein n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3H897_9STRA)

HSP 1 Score: 137 bits (344), Expect = 4.000e-31
Identity = 112/369 (30.35%), Postives = 152/369 (41.19%), Query Frame = 0

Query:   92 FDHSRWRKRTVAFQLMYEGGGYAGFCSQSEGNEDTIEKRLFHAFTTTKLVERIATGDNYSRCGRTDRGVSALGNVVTVSARSKFPKELDVE---LPTSPLGSVRVVSGGGXXXXXXXXXXXXRDEGSGSXXXXXXXXXXXXXXXXXXRTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGDGREGMEREARMGDVERSSGGGGRGXXXXXXXXXXXXXXXXXXXXXSGGRECEASGQDASNGKATGEADKXXXXXXXXXXXXXKGDGTVEVTEIDYCGVLNKVLPPDIRALAWAPVTEGFSARFSCSDRTYRQEKAYFFLARDLDIEAMDEAASRIVGHHDFRNLCKIDVANVSNFVRFIKFASVR 457
            F  S++R R +A Q+ Y+G  Y GF SQ    E+T+E  LF+A    KL+E   T   YSRCGRTD+GVSALG V++V+ RS  P++L  E   +  +P G   +                                                                                    E  A    V  +S  G                        S   + + SG++    K+   A                G  T  +TE+DY  +LN+ LP DIR L W  VTE FSARFS SDR YR    YFF  + L+I AM +AA+ ++G+HDFRNLCK+D+ANV+NF R I  A ++
Sbjct:   78 FSLSKYRTRQIAMQVQYDGSAYLGFASQDL--EETVENHLFNALLKLKLIEDRKTCQ-YSRCGRTDKGVSALGQVISVNVRSALPRDLPEEALVVLRNPTGKYEIT-----------------------------------------------------------------------------------EPAA----VNSTSSAGN-----------------------STAEQADNSGKEPPRKKSKKNATSDTPA----------GPKTKIITEMDYVTMLNRTLPDDIRILGWCEVTEEFSARFSASDRKYR----YFFPRKTLNIAAMQQAATYLLGNHDFRNLCKMDIANVTNFRRDIYSAEIK 319

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BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot
Match: A0A7R9Y7Q5_9STRA (Hypothetical protein n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9Y7Q5_9STRA)

HSP 1 Score: 134 bits (338), Expect = 1.160e-30
Identity = 72/124 (58.06%), Postives = 82/124 (66.13%), Query Frame = 0

Query:  356 GDGTVEVTEIDYCGVLNKVLPPDIRALAWAPVTEGFSARFSCSDRTYRQEKAYFFLARDLDIEAMDEAASRIVGHHDFRNLCKIDVANVSNFVRFIKFASVRPVPSPSPDYGGTEYYRMCVFEV 479
            G+ T E  EIDYCG+LN+VLP  IR LAWAPVTE FSARFS   R+YR    YFF AR LD+ AMDEAA  ++G HDFRN CK+DV NV NF R +  A+VR V        G    RM VFEV
Sbjct:  149 GERTQEKREIDYCGLLNRVLPEAIRVLAWAPVTEDFSARFSAESRSYR----YFFSARGLDLSAMDEAARLLIGSHDFRNFCKMDVVNVRNFRRRVYRAAVRLVGHAEALAPGNSAPRMAVFEV 268

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BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot
Match: A0A7L4DBW5_9AVES (PUS3 synthase (Fragment) n=2 Tax=Coraciiformes TaxID=8936 RepID=A0A7L4DBW5_9AVES)

HSP 1 Score: 126 bits (317), Expect = 2.960e-28
Identity = 69/120 (57.50%), Postives = 81/120 (67.50%), Query Frame = 0

Query:  361 EVTEIDYCGVLNKVLPPDIRALAWAPVTEGFSARFSCSDRTYRQEKAYFFLARDLDIEAMDEAASRIVGHHDFRNLCKIDVAN-VSNFVRFIKFASVRPVPSPSPDYGGTEYYRMCVFEV 479
            E  E+ Y  +LN+VLPPDIRALAWAPV  GFSARFSC  RTYR    YFF   DLD++ M+ AA R VG HDFRNLCK+DVAN V NF R I  A+V  V     + G  + +R+C FEV
Sbjct:  160 EEEELRYTHILNRVLPPDIRALAWAPVEPGFSARFSCLKRTYR----YFFPCADLDVDLMNAAAQRYVGTHDFRNLCKMDVANGVVNFQRTILSAAVTWVERGGGETGLQDPFRLCQFEV 275

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BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot
Match: K7EAA9_ORNAN (Pseudouridine synthase 3 n=2 Tax=Monotremata TaxID=9255 RepID=K7EAA9_ORNAN)

HSP 1 Score: 126 bits (316), Expect = 8.640e-28
Identity = 69/117 (58.97%), Postives = 81/117 (69.23%), Query Frame = 0

Query:  364 EIDYCGVLNKVLPPDIRALAWAPVTEGFSARFSCSDRTYRQEKAYFFLARDLDIEAMDEAASRIVGHHDFRNLCKIDVAN-VSNFVRFIKFASVRPVPSPSPDYGGTEYYRMCVFEV 479
            EI Y  +LN+VLPPDIR LAWAPV  GFSARF+C  RTYR    YFF   DLD+EAMD AA + VG HDFRNLCK+DVAN V++F R I  A VR V   S +    + +R+C FEV
Sbjct:  154 EIRYTHILNRVLPPDIRVLAWAPVGSGFSARFNCLQRTYR----YFFPRADLDLEAMDRAAQKYVGTHDFRNLCKMDVANGVTSFQRTILSAQVRRVDQ-SEETDRRDPFRLCQFEV 265

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BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot
Match: A0A7L4MY80_9AVES (tRNA pseudouridine synthase (Fragment) n=1 Tax=Ceyx cyanopectus TaxID=390723 RepID=A0A7L4MY80_9AVES)

HSP 1 Score: 121 bits (304), Expect = 1.070e-27
Identity = 67/117 (57.26%), Postives = 79/117 (67.52%), Query Frame = 0

Query:  364 EIDYCGVLNKVLPPDIRALAWAPVTEGFSARFSCSDRTYRQEKAYFFLARDLDIEAMDEAASRIVGHHDFRNLCKIDVAN-VSNFVRFIKFASVRPVPSPSPDYGGTEYYRMCVFEV 479
            E+ Y  +LN+VLPPDIR LAWAPV  GFSARFSC  RTYR    YFF   +LD+E M+ AA R VG HDFRNLCK+DVAN V NF R I  A+V  V     + G  + +R+C FEV
Sbjct:    3 ELRYTHILNRVLPPDIRVLAWAPVEPGFSARFSCLQRTYR----YFFPCANLDVELMNSAAQRYVGSHDFRNLCKMDVANGVINFQRTILSATVTWVEKGG-ETGPWDPFRLCQFEV 114

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BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot
Match: A0A401QE62_SCYTO (PseudoU_synth_1 domain-containing protein n=1 Tax=Scyliorhinus torazame TaxID=75743 RepID=A0A401QE62_SCYTO)

HSP 1 Score: 122 bits (306), Expect = 1.240e-27
Identity = 68/123 (55.28%), Postives = 76/123 (61.79%), Query Frame = 0

Query:  364 EIDYCGVLNKVLPPDIRALAWAPVTEGFSARFSCSDRTYRQEKAYFFLARDLDIEAMDEAASRIVGHHDFRNLCKIDVAN-VSNFVRFIKFASVRPVPSPSPDYGGT------EYYRMCVFEV 479
            E+ Y  +LN+VLPPDIRAL WAPV   FSARFSC  RTYR    Y+F    LD+  MDEAA R  G HDFRNLCK+DVAN V NF R +  A V PVP P  + GG         Y MC  EV
Sbjct:  136 ELRYTHILNRVLPPDIRALGWAPVGRDFSARFSCLHRTYR----YYFPGAGLDLARMDEAARRFEGAHDFRNLCKMDVANGVLNFRRTVLHARVGPVPPPGQEAGGACPGMRPSPYGMCQLEV 254

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BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot
Match: A0A7E6CV69_9CHIR (tRNA pseudouridine(38/39) synthase isoform X2 n=2 Tax=Phyllostomus discolor TaxID=89673 RepID=A0A7E6CV69_9CHIR)

HSP 1 Score: 123 bits (309), Expect = 2.240e-27
Identity = 68/117 (58.12%), Postives = 79/117 (67.52%), Query Frame = 0

Query:  364 EIDYCGVLNKVLPPDIRALAWAPVTEGFSARFSCSDRTYRQEKAYFFLARDLDIEAMDEAASRIVGHHDFRNLCKIDVAN-VSNFVRFIKFASVRPVPSPSPDYGGTEYYRMCVFEV 479
            EI Y  +LN+VLPPDIR LAWAPV  GFSARFSC +RTYR    YFF   DLDI  M+ AA + VG HDFRNLCK+DVAN V NF R I  A V+ V     + G  E +++C FEV
Sbjct:  156 EIRYTHILNRVLPPDIRVLAWAPVEPGFSARFSCLERTYR----YFFPRADLDIVTMNCAAHKYVGTHDFRNLCKMDVANGVINFQRTILSAEVKLVGQSLAEDGRQEPFQLCQFEV 268

[Close]

BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot
Match: A0A452EBW3_CAPHI (Pseudouridine synthase 3 n=5 Tax=Capra hircus TaxID=9925 RepID=A0A452EBW3_CAPHI)

HSP 1 Score: 124 bits (310), Expect = 2.280e-27
Identity = 67/118 (56.78%), Postives = 80/118 (67.80%), Query Frame = 0

Query:  363 TEIDYCGVLNKVLPPDIRALAWAPVTEGFSARFSCSDRTYRQEKAYFFLARDLDIEAMDEAASRIVGHHDFRNLCKIDVAN-VSNFVRFIKFASVRPVPSPSPDYGGTEYYRMCVFEV 479
            TEI Y  +LN+VLPPDIR LAWAPV   FSARFSC +RTYR    YFF   +LDI  M+ AA + VG HDFRNLCK+DVAN V+NF R I  A V+ V     + G  E +++C FEV
Sbjct:  103 TEIRYTHILNRVLPPDIRVLAWAPVETSFSARFSCLERTYR----YFFPCANLDIVTMNYAAQKYVGTHDFRNLCKMDVANGVTNFQRTILSAQVQRVGQNLAEEGWQEPFQLCQFEV 216

The following BLAST results are available for this feature:

BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot

BLAST of mRNA_M-pyrifera_M_contig10890.1869.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
A0A6H5JEX6_9PHAE	8.180e-101	53.54	PseudoU_synth_1 domain-containing protein n=1 Tax=...	[more]
D7FHT8_ECTSI	1.620e-82	53.68	Pseudouridylate synthase 3 n=1 Tax=Ectocarpus sili...	[more]
A0A7S3H897_9STRA	4.000e-31	30.35	Hypothetical protein n=1 Tax=Spumella elongata Tax...	[more]
A0A7R9Y7Q5_9STRA	1.160e-30	58.06	Hypothetical protein n=1 Tax=Pinguiococcus pyrenoi...	[more]
A0A7L4DBW5_9AVES	2.960e-28	57.50	PUS3 synthase (Fragment) n=2 Tax=Coraciiformes Tax...	[more]
K7EAA9_ORNAN	8.640e-28	58.97	Pseudouridine synthase 3 n=2 Tax=Monotremata TaxID...	[more]
A0A7L4MY80_9AVES	1.070e-27	57.26	tRNA pseudouridine synthase (Fragment) n=1 Tax=Cey...	[more]
A0A401QE62_SCYTO	1.240e-27	55.28	PseudoU_synth_1 domain-containing protein n=1 Tax=...	[more]
A0A7E6CV69_9CHIR	2.240e-27	58.12	tRNA pseudouridine(38/39) synthase isoform X2 n=2 ...	[more]
A0A452EBW3_CAPHI	2.280e-27	56.78	Pseudouridine synthase 3 n=5 Tax=Capra hircus TaxI...	[more]

Pages

InterPro

Analysis Name: InterProScan on OGS1.0 of Macrocystis pyrifera male
Date Performed: 2022-09-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020095	Pseudouridine synthase I, TruA, C-terminal	GENE3D	3.30.70.660		coord: 394..471 e-value: 4.4E-6 score: 28.4
None	No IPR available	GENE3D	3.30.70.580		coord: 89..195 e-value: 1.0E-18 score: 69.2
None	No IPR available	PANTHER	PTHR11142:SF5	TRNA PSEUDOURIDINE(38/39) SYNTHASE	coord: 348..479
None	No IPR available	PANTHER	PTHR11142:SF5	TRNA PSEUDOURIDINE(38/39) SYNTHASE	coord: 65..180
IPR001406	Pseudouridine synthase I, TruA	PANTHER	PTHR11142	PSEUDOURIDYLATE SYNTHASE	coord: 348..479 coord: 65..180
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 98..178
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 361..458

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
M-pyrifera_M_contig10890	contig	M-pyrifera_M_contig10890:528..8252 -

Analyses

This polypeptide is derived from or has results from the following analyses


Analysis Name	Date Performed
InterProScan on OGS1.0 of Macrocystis pyrifera male	2022-09-29
Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef90	2022-09-16
OGS1.0 of Macrocystis pyrifera P11B4 male	2021-02-24

Relationships

This polypeptide derives from the following mRNA feature(s):


Feature Name	Unique Name	Species	Type	Position
mRNA_M-pyrifera_M_contig10890.1869.1	mRNA_M-pyrifera_M_contig10890.1869.1	Macrocystis pyrifera P11B4 male	mRNA	M-pyrifera_M_contig10890 528..8252 -

Sequences

The following sequences are available for this feature:

polypeptide sequence

polypeptide sequence

>prot_M-pyrifera_M_contig10890.1869.1 ID=prot_M-pyrifera_M_contig10890.1869.1|Name=mRNA_M-pyrifera_M_contig10890.1869.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=479bp
GDSGGSGDGASASADVAAAETSSEVSATAPSSFGVSGVPSGFDGARVPPA
ISARGLSWKEKQRRKKVVAAAAAAAGVVAGKGKKKRGQERDFDHSRWRKR
TVAFQLMYEGGGYAGFCSQSEGNEDTIEKRLFHAFTTTKLVERIATGDNY
SRCGRTDRGVSALGNVVTVSARSKFPKELDVELPTSPLGSVRVVSGGGSG
GGDGGGGGGGRDEGSGSDGGGGDGGDGGGGVEGGDRTSGAEGPGAVLGGD
AETSAEGGKAATDGDGARSVGDGREGMEREARMGDVERSSGGGGRGGGGG
DGAGGGDGGGEGVGGDASGGRECEASGQDASNGKATGEADKKAKKSKKKD
KKKKKGDGTVEVTEIDYCGVLNKVLPPDIRALAWAPVTEGFSARFSCSDR
TYRQEKAYFFLARDLDIEAMDEAASRIVGHHDFRNLCKIDVANVSNFVRF
IKFASVRPVPSPSPDYGGTEYYRMCVFEV

Annotated Terms

The following terms have been associated with this polypeptide:

Vocabulary: **INTERPRO**
Term	Definition
IPR020095	PsdUridine_synth_TruA_C
IPR001406	PsdUridine_synth_TruA
IPR020103	PsdUridine_synth_cat_dom_sf