mRNA_M-pyrifera_M_contig9980.22949.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig9980.22949.1
Unique NamemRNA_M-pyrifera_M_contig9980.22949.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig9980.22949.1 vs. uniprot
Match: D7G5W4_ECTSI (STE20-like serine/threonine kinase Ste20-like serine/threonine protein kinase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5W4_ECTSI)

HSP 1 Score: 149 bits (376), Expect = 5.840e-45
Identity = 69/73 (94.52%), Postives = 71/73 (97.26%), Query Frame = 1 
Query:    1 WPTGADGYKLDKMIGKGAFATVWKAFCAARGVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 219
            WPTGADGYKLD++IGKGAFATVWKAFC AR VHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF
Sbjct:   14 WPTGADGYKLDRIIGKGAFATVWKAFCEAREVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 86
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BLAST of mRNA_M-pyrifera_M_contig9980.22949.1 vs. uniprot
Match: A0A6H5JLL3_9PHAE (Ste20-like protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JLL3_9PHAE)

HSP 1 Score: 149 bits (376), Expect = 1.200e-41
Identity = 69/73 (94.52%), Postives = 71/73 (97.26%), Query Frame = 1 
Query:    1 WPTGADGYKLDKMIGKGAFATVWKAFCAARGVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 219
            WPTGADGYKLD++IGKGAFATVWKAFC AR VHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF
Sbjct:   27 WPTGADGYKLDRIIGKGAFATVWKAFCEAREVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 99
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BLAST of mRNA_M-pyrifera_M_contig9980.22949.1 vs. uniprot
Match: A0A835Z4G7_9STRA (Protein kinase domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z4G7_9STRA)

HSP 1 Score: 120 bits (301), Expect = 9.700e-31
Identity = 53/73 (72.60%), Postives = 62/73 (84.93%), Query Frame = 1 
Query:    1 WPTGADGYKLDKMIGKGAFATVWKAFCAARGVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 219
            WP  A+GY+L+K IG+GAFATVWKAFC  R   VA+K+MDLENVTH+FEDIRQEV VMR+CDHPNV+ CY SF
Sbjct:   16 WPCTAEGYELEKQIGQGAFATVWKAFCFDRNQPVAIKIMDLENVTHAFEDIRQEVNVMRLCDHPNVVRCYCSF 88
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BLAST of mRNA_M-pyrifera_M_contig9980.22949.1 vs. uniprot
Match: M4BWB8_HYAAE (Protein kinase domain-containing protein n=7 Tax=Peronosporaceae TaxID=4777 RepID=M4BWB8_HYAAE)

HSP 1 Score: 106 bits (264), Expect = 8.380e-26
Identity = 46/73 (63.01%), Postives = 57/73 (78.08%), Query Frame = 1 
Query:    1 WPTGADGYKLDKMIGKGAFATVWKAFCAARGVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 219
            WPT A  YK+ + IG+GAFA VWKA+C A+ +HVA+K+MDLE +T SFEDIR EV  M+M +HPNVL CY SF
Sbjct:    5 WPTVASEYKIIEQIGQGAFAKVWKAYCVAKKIHVAIKIMDLEKITTSFEDIRAEVQTMKMTNHPNVLKCYCSF 77
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BLAST of mRNA_M-pyrifera_M_contig9980.22949.1 vs. uniprot
Match: A0A024GBY1_9STRA (Protein kinase domain-containing protein n=1 Tax=Albugo candida TaxID=65357 RepID=A0A024GBY1_9STRA)

HSP 1 Score: 98.6 bits (244), Expect = 1.730e-25
Identity = 44/73 (60.27%), Postives = 53/73 (72.60%), Query Frame = 1 
Query:    1 WPTGADGYKLDKMIGKGAFATVWKAFCAARGVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 219
            WPT A  Y+L + IG+GAFA VWKA C  + V VA+K+MDLE +T SFEDIR EV  M+M +HPNVL CY  F
Sbjct:    6 WPTNASDYRLVEQIGQGAFAKVWKAHCIKKNVDVALKIMDLEKITTSFEDIRAEVQTMKMTNHPNVLKCYCRF 78
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BLAST of mRNA_M-pyrifera_M_contig9980.22949.1 vs. uniprot
Match: A0A2D4BF87_PYTIN (Protein kinase, putative n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BF87_PYTIN)

HSP 1 Score: 103 bits (257), Expect = 5.280e-25
Identity = 45/73 (61.64%), Postives = 56/73 (76.71%), Query Frame = 1 
Query:    1 WPTGADGYKLDKMIGKGAFATVWKAFCAARGVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 219
            WPT A  Y++ + IG+GAFA VWKA+CA +  HVA+K+MDLE +T SFEDIR EV  M+M +HPNVL CY SF
Sbjct:    5 WPTVASDYQIIEQIGQGAFAKVWKAYCAPKKTHVAIKIMDLEKITTSFEDIRAEVQTMKMTNHPNVLKCYCSF 77
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BLAST of mRNA_M-pyrifera_M_contig9980.22949.1 vs. uniprot
Match: H3HC15_PHYRM (Protein kinase domain-containing protein n=25 Tax=Oomycota TaxID=4762 RepID=H3HC15_PHYRM)

HSP 1 Score: 104 bits (259), Expect = 5.510e-25
Identity = 45/73 (61.64%), Postives = 57/73 (78.08%), Query Frame = 1 
Query:    1 WPTGADGYKLDKMIGKGAFATVWKAFCAARGVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 219
            WPT A  Y++ + IG+GAFA VWKA+CA + +HVA+K+MDLE +T SFEDIR EV  M+M +HPNVL CY SF
Sbjct:    5 WPTVASEYQIIEQIGQGAFAKVWKAYCAPKKIHVAIKIMDLEKITTSFEDIRAEVQTMKMTNHPNVLKCYCSF 77
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BLAST of mRNA_M-pyrifera_M_contig9980.22949.1 vs. uniprot
Match: A0A5D6YGQ3_9STRA (Protein kinase domain-containing protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6YGQ3_9STRA)

HSP 1 Score: 102 bits (254), Expect = 7.460e-25
Identity = 45/73 (61.64%), Postives = 56/73 (76.71%), Query Frame = 1 
Query:    1 WPTGADGYKLDKMIGKGAFATVWKAFCAARGVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 219
            WPT A  Y++ + IG+GAFA VWKA+CA +  HVA+K+MDLE +T SFEDIR EV  M+M +HPNVL CY SF
Sbjct:    5 WPTVAAEYQIIEQIGQGAFAKVWKAYCAPKKAHVAIKIMDLEKITTSFEDIRAEVQTMKMTNHPNVLKCYCSF 77
[Close]
BLAST of mRNA_M-pyrifera_M_contig9980.22949.1 vs. uniprot
Match: A0A662WTR8_9STRA (Protein kinase domain-containing protein n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662WTR8_9STRA)

HSP 1 Score: 102 bits (253), Expect = 1.610e-24
Identity = 44/73 (60.27%), Postives = 56/73 (76.71%), Query Frame = 1 
Query:    1 WPTGADGYKLDKMIGKGAFATVWKAFCAARGVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 219
            WPT A  Y++ + IG+GAFA VWKA+C  + +HVA+K+MDLE +T SFEDIR EV  M+M +HPNVL CY SF
Sbjct:   46 WPTVAAEYQIIEQIGQGAFAKVWKAYCVPKKIHVAIKIMDLEKITTSFEDIRAEVQTMKMTNHPNVLKCYCSF 118
[Close]
BLAST of mRNA_M-pyrifera_M_contig9980.22949.1 vs. uniprot
Match: A0A8K1CM74_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CM74_PYTOL)

HSP 1 Score: 101 bits (252), Expect = 5.880e-24
Identity = 44/73 (60.27%), Postives = 55/73 (75.34%), Query Frame = 1 
Query:    1 WPTGADGYKLDKMIGKGAFATVWKAFCAARGVHVAVKVMDLENVTHSFEDIRQEVAVMRMCDHPNVLSCYASF 219
            WPT A  Y++ + IG+GAFA VWKA+C  +  HVA+K+MDLE +T SFEDIR EV  M+M +HPNVL CY SF
Sbjct:    5 WPTVASDYQIIEQIGQGAFAKVWKAYCIPKKTHVAIKIMDLEKITTSFEDIRAEVQTMKMTNHPNVLKCYCSF 77
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig9980.22949.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G5W4_ECTSI5.840e-4594.52STE20-like serine/threonine kinase Ste20-like seri... [more]
A0A6H5JLL3_9PHAE1.200e-4194.52Ste20-like protein n=1 Tax=Ectocarpus sp. CCAP 131... [more]
A0A835Z4G7_9STRA9.700e-3172.60Protein kinase domain-containing protein n=1 Tax=T... [more]
M4BWB8_HYAAE8.380e-2663.01Protein kinase domain-containing protein n=7 Tax=P... [more]
A0A024GBY1_9STRA1.730e-2560.27Protein kinase domain-containing protein n=1 Tax=A... [more]
A0A2D4BF87_PYTIN5.280e-2561.64Protein kinase, putative n=1 Tax=Pythium insidiosu... [more]
H3HC15_PHYRM5.510e-2561.64Protein kinase domain-containing protein n=25 Tax=... [more]
A0A5D6YGQ3_9STRA7.460e-2561.64Protein kinase domain-containing protein n=1 Tax=P... [more]
A0A662WTR8_9STRA1.610e-2460.27Protein kinase domain-containing protein n=2 Tax=N... [more]
A0A8K1CM74_PYTOL5.880e-2460.27Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig9980contigM-pyrifera_M_contig9980:5882..6822 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score106.7
Seed ortholog evalue4.7e-21
Seed eggNOG ortholog272952.HpaP810819
Preferred nameSTK39
KEGG koko:K08835
Hectar predicted targeting categoryother localisation
GOsGO:0000166,GO:0000187,GO:0000287,GO:0001885,GO:0001894,GO:0001932,GO:0001933,GO:0001934,GO:0002009,GO:0002028,GO:0002064,GO:0003008,GO:0003013,GO:0003158,GO:0003674,GO:0003824,GO:0004672,GO:0004674,GO:0005488,GO:0005515,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005829,GO:0005856,GO:0005886,GO:0006355,GO:0006357,GO:0006464,GO:0006468,GO:0006793,GO:0006796,GO:0006807,GO:0006950,GO:0006970,GO:0006971,GO:0006972,GO:0006979,GO:0007154,GO:0007165,GO:0007272,GO:0007275,GO:0007346,GO:0007399,GO:0007422,GO:0007444,GO:0008015,GO:0008065,GO:0008104,GO:0008144,GO:0008150,GO:0008152,GO:0008217,GO:0008362,GO:0008366,GO:0009628,GO:0009651,GO:0009653,GO:0009888,GO:0009889,GO:0009891,GO:0009892,GO:0009893,GO:0009966,GO:0009967,GO:0009987,GO:0010468,GO:0010556,GO:0010557,GO:0010562,GO:0010563,GO:0010604,GO:0010605,GO:0010628,GO:0010646,GO:0010647,GO:0010766,GO:0010941,GO:0010959,GO:0016020,GO:0016301,GO:0016310,GO:0016323,GO:0016324,GO:0016740,GO:0016772,GO:0016773,GO:0017076,GO:0018105,GO:0018107,GO:0018193,GO:0018209,GO:0018210,GO:0019219,GO:0019220,GO:0019222,GO:0019538,GO:0019898,GO:0019899,GO:0019900,GO:0019901,GO:0022898,GO:0023014,GO:0023016,GO:0023051,GO:0023052,GO:0023056,GO:0030104,GO:0030154,GO:0030554,GO:0030855,GO:0031098,GO:0031323,GO:0031324,GO:0031325,GO:0031326,GO:0031328,GO:0031347,GO:0031399,GO:0031400,GO:0031401,GO:0031974,GO:0031981,GO:0032101,GO:0032147,GO:0032268,GO:0032269,GO:0032270,GO:0032409,GO:0032410,GO:0032411,GO:0032412,GO:0032413,GO:0032414,GO:0032501,GO:0032502,GO:0032553,GO:0032555,GO:0032559,GO:0032879,GO:0032890,GO:0032891,GO:0033036,GO:0033554,GO:0033674,GO:0034613,GO:0034762,GO:0034763,GO:0034764,GO:0034765,GO:0034766,GO:0034767,GO:0035220,GO:0035239,GO:0035295,GO:0035556,GO:0035639,GO:0036003,GO:0036094,GO:0036211,GO:0036251,GO:0036438,GO:0040003,GO:0042325,GO:0042326,GO:0042327,GO:0042335,GO:0042538,GO:0042592,GO:0042802,GO:0042981,GO:0043067,GO:0043085,GO:0043167,GO:0043168,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043266,GO:0043267,GO:0043268,GO:0043269,GO:0043270,GO:0043271,GO:0043405,GO:0043406,GO:0043408,GO:0043410,GO:0043412,GO:0043549,GO:0043618,GO:0043620,GO:0044057,GO:0044058,GO:0044070,GO:0044092,GO:0044093,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044444,GO:0044446,GO:0044459,GO:0044464,GO:0045177,GO:0045446,GO:0045859,GO:0045860,GO:0045893,GO:0045935,GO:0045936,GO:0045937,GO:0045944,GO:0046777,GO:0046872,GO:0048468,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0048585,GO:0048729,GO:0048731,GO:0048856,GO:0048869,GO:0048871,GO:0048878,GO:0050727,GO:0050789,GO:0050790,GO:0050794,GO:0050801,GO:0050878,GO:0050891,GO:0050896,GO:0051046,GO:0051048,GO:0051049,GO:0051050,GO:0051051,GO:0051171,GO:0051172,GO:0051173,GO:0051174,GO:0051179,GO:0051239,GO:0051241,GO:0051246,GO:0051247,GO:0051248,GO:0051252,GO:0051254,GO:0051338,GO:0051347,GO:0051641,GO:0051716,GO:0051726,GO:0051952,GO:0051953,GO:0051955,GO:0051956,GO:0060249,GO:0060255,GO:0060429,GO:0060457,GO:0060562,GO:0061392,GO:0061393,GO:0061404,GO:0061416,GO:0065007,GO:0065008,GO:0065009,GO:0070013,GO:0070727,GO:0071214,GO:0071470,GO:0071472,GO:0071474,GO:0071475,GO:0071476,GO:0071704,GO:0071900,GO:0071902,GO:0071944,GO:0072697,GO:0080090,GO:0080134,GO:0090186,GO:0090188,GO:0097159,GO:0097367,GO:0098590,GO:0104004,GO:0140096,GO:1901016,GO:1901017,GO:1901265,GO:1901363,GO:1901379,GO:1901380,GO:1901564,GO:1902305,GO:1902306,GO:1902531,GO:1902533,GO:1902680,GO:1903506,GO:1903508,GO:1903789,GO:1903792,GO:1903959,GO:1903960,GO:1904062,GO:1904063,GO:1905407,GO:1905408,GO:1990778,GO:2000112,GO:2000649,GO:2000650,GO:2001023,GO:2001024,GO:2001141
EggNOG free text desc.Serine/Threonine protein kinases, catalytic domain
EggNOG OGs3Q70Y@4776,KOG0582@1,KOG0582@2759
EC2.7.11.1
COG Functional cat.T
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko01001
Exons2
Model size219
Cds size219
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816915.2592692-CDS-M-pyrifera_M_contig9980:5881..59381622816915.2592692-CDS-M-pyrifera_M_contig9980:5881..5938Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9980 5882..5938 -
1692278253.088266-CDS-M-pyrifera_M_contig9980:5881..59381692278253.088266-CDS-M-pyrifera_M_contig9980:5881..5938Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9980 5882..5938 -
1622816915.271067-CDS-M-pyrifera_M_contig9980:6660..68221622816915.271067-CDS-M-pyrifera_M_contig9980:6660..6822Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9980 6661..6822 -
1692278253.0994759-CDS-M-pyrifera_M_contig9980:6660..68221692278253.0994759-CDS-M-pyrifera_M_contig9980:6660..6822Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9980 6661..6822 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig9980.22949.1prot_M-pyrifera_M_contig9980.22949.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig9980 5882..6822 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig9980.22949.1

>prot_M-pyrifera_M_contig9980.22949.1 ID=prot_M-pyrifera_M_contig9980.22949.1|Name=mRNA_M-pyrifera_M_contig9980.22949.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=73bp
WPTGADGYKLDKMIGKGAFATVWKAFCAARGVHVAVKVMDLENVTHSFED
IRQEVAVMRMCDHPNVLSCYASF
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mRNA from alignment at M-pyrifera_M_contig9980:5882..6822-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig9980.22949.1 ID=mRNA_M-pyrifera_M_contig9980.22949.1|Name=mRNA_M-pyrifera_M_contig9980.22949.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=941bp|location=Sequence derived from alignment at M-pyrifera_M_contig9980:5882..6822- (Macrocystis pyrifera P11B4 male)
TGGCCTACCGGTGCTGATGGTTACAAGTTAGACAAGATGATCGGAAAAGG
AGCGTTCGCAACAGTATGGAAAGCATTTTGTGCAGCGAGGGGGGTGCACG
TGGCGGTCAAGGTGATGGATCTCGAGAACGTTACGCATAGCTTCGAGGAC
ATCCGTCAAGAGGTTGGTGGTTTTGACCGTTATGCCTTTTTTTGCTGCCT
TCCGCGAACCGCTAGAGGTTACCCTCGGGTGTGTTTAGCATTAGACCAAA
ATTGCGAAATTCAGCAGCTACCTTTGAGCATCATGCAAGTGTTGGTGAGT
TGCAGCGAAATTTTCGTTTCTTGTTGTTGTCGGCTCATCGCATACCTGGT
AGTGTAATGAATTGACACAACATTGGGCAGGATCCAAAGTTTGGAGGTGA
TACGGTGCCGAAGTGCGCAATGCAGCCTTGCGTTGCTCTCCTCCTTTTGT
TTGTGGTTCATTTAAAGCCACGTCGTGTAGAGACCTAGCCTCGAATTCGC
CTTAACACTGTCTGCGGGCTTCAGCCATGCTTTCAGCTTGCATGTTACGC
GGCAAAAGTGTTGACAATCTCGAACATTGAATTTCTGAAAATTCCCGGCA
CATCATGGCACCAGTGCACAACCTCTCTTAACTTGCCGGAAGCCGTCATT
ATGTGTTACTGATATGCTCTGCTATGTGAAGGTTGTGCTTGAGCTGCACT
TATACTTCATATCGTTAATGCATTGTTATTATAGGCGCAGAATTACGTTT
CGTCGACAGGAACAATGTAGTAACATTCCAGGAATCTCAGTGTTTGGGTC
GTCACTAGCGTGCAGGTCGATTTGCGCAGCGTAACTCATTCAGGTGCTCT
TTGTTTTGCTTCACCTGTTTTATATTGTTAGCAGGTGGCGGTAATGCGCA
TGTGCGACCACCCGAATGTGCTGAGCTGCTACGCATCGTTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig9980:5882..6822-

>mRNA_M-pyrifera_M_contig9980.22949.1 ID=mRNA_M-pyrifera_M_contig9980.22949.1|Name=mRNA_M-pyrifera_M_contig9980.22949.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=438bp|location=Sequence derived from alignment at M-pyrifera_M_contig9980:5882..6822- (Macrocystis pyrifera P11B4 male)
TGGCCTACCGGTGCTGATGGTTACAAGTTAGACAAGATGATCGGAAAAGG
AGCGTTCGCAACAGTATGGAAAGCATTTTGTGCAGCGAGGGGGGTGCACG
TGGCGGTCAAGGTGATGGATCTCGAGAACGTTACGCATAGCTTCGAGGAC
ATCCGTCAAGAGTGGCCTACCGGTGCTGATGGTTACAAGTTAGACAAGAT
GATCGGAAAAGGAGCGTTCGCAACAGTATGGAAAGCATTTTGTGCAGCGA
GGGGGGTGCACGTGGCGGTCAAGGTGATGGATCTCGAGAACGTTACGCAT
AGCTTCGAGGACATCCGTCAAGAGGTGGCGGTAATGCGCATGTGCGACCA
CCCGAATGTGCTGAGCTGCTACGCATCGTTCGTGGCGGTAATGCGCATGT
GCGACCACCCGAATGTGCTGAGCTGCTACGCATCGTTC
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