mRNA_M-pyrifera_M_contig99449.22876.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig99449.22876.1
Unique NamemRNA_M-pyrifera_M_contig99449.22876.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig99449.22876.1 vs. uniprot
Match: A0A2H9TPG7_9FUNG (Transitional endoplasmic reticulum ATPase TER94-like protein n=1 Tax=Paramicrosporidium saccamoebae TaxID=1246581 RepID=A0A2H9TPG7_9FUNG)

HSP 1 Score: 135 bits (341), Expect = 8.840e-34
Identity = 71/173 (41.04%), Postives = 100/173 (57.80%), Query Frame = 1
Query:    1 DDLTWFVHVSDLHLSKF-NAVRTERFELFLEEGLEVISPSFVLVTGDLVDAKHTDKVRSEQFPEEWATYKKLLSEHGWWD-PDKWIDLRGNHDCFGIGANWEQPQNLFREWSVTGSHFHGPVREFSFIRDGASDRPLVDVVTLDACPIVGAKTPLNFFGRMSTTQLDRLEVEV 513
            D L WFV VSD+H++ F +   +E  E FL E L +ISP FV+ TGDL DAK +  + S+Q   EW TY+KLL   G +D P+ W D+RGNHDCF +   W   QNLF  +S   S  +  + +  F + G        ++ +D CP  G   P NFFG ++T ++D LE ++
Sbjct: 1183 DHLFWFVQVSDIHVNHFGHGFGSEHLEYFLRETLPIISPLFVVATGDLTDAKDSPGLGSKQHRVEWETYQKLLQRSGVYDRPNFWFDIRGNHDCFDV-PGWSSAQNLFSHYSKMKSSSYSHIFKLPFGKYG--------LIAVDGCPEYGPSRPFNFFGALTTNKMDVLEQQL 1346          
BLAST of mRNA_M-pyrifera_M_contig99449.22876.1 vs. uniprot
Match: A0A397H154_9GLOM (Metallophos domain-containing protein n=1 Tax=Diversispora epigaea TaxID=1348612 RepID=A0A397H154_9GLOM)

HSP 1 Score: 132 bits (333), Expect = 7.890e-33
Identity = 74/171 (43.27%), Postives = 96/171 (56.14%), Query Frame = 1
Query:    1 DDLTWFVHVSDLHLSKFNAVR-TERFELFLEEGLEVISPSFVLVTGDLVDAKHTDKVRSEQFPEEWATYKKLLSEHGWWDPDK-WIDLRGNHDCFGIGANWEQPQNLFREWSVTGSHFHGPV--REFSFIRDGASDRPLVDVVTLDACPIVGAKTPLNFFGRMSTTQLDRL 501
            D++ +F+ +SDLH+SKF+    T  F  FL   L V+SPSFVLVTGDL D K  D + S+Q  EEW +Y+  L E G  D    W DLRGNHDCF +  +WE  QNLFR++S         +  +EF   R           + +DACP +G   P NFFG   T  +DRL
Sbjct:   76 DNVFYFIQISDLHISKFHKTGGTSHFLHFLGTILPVVSPSFVLVTGDLTDGKDVDGITSQQLIEEWTSYQTALKESGVLDRSNYWFDLRGNHDCFNV-RSWESEQNLFRKFSALKQQGFDVIIEKEFGNYR----------FLGIDACPKIGPARPFNFFGYYETEDMDRL 235          
BLAST of mRNA_M-pyrifera_M_contig99449.22876.1 vs. uniprot
Match: A0A0D2URX7_CAPO3 (Metallophos domain-containing protein n=1 Tax=Capsaspora owczarzaki (strain ATCC 30864) TaxID=595528 RepID=A0A0D2URX7_CAPO3)

HSP 1 Score: 131 bits (330), Expect = 2.350e-32
Identity = 70/169 (41.42%), Postives = 95/169 (56.21%), Query Frame = 1
Query:    1 DDLTWFVHVSDLHLSKF-NAVRTERFELFLEEGLEVISPSFVLVTGDLVDAKHTDKVRSEQFPEEWATYKKLLSEHGWWDPDKWIDLRGNHDCFGIGANWEQPQNLFREWSVTGSHFHGPVREFSFIRDGASDRPLVDVVTLDACPIVGAKTPLNFFGRMSTTQLDRLE 504
            D++ WF+HVSDLH+S++ N  RT  FE F  E + VI+P  V+VTGDL DAK    + S Q  EEW  Y   L   G  D  KW+D+RGNHD F + A  + P NLF  +SV+ +  +  V + +F R           V +DACP  G + P NFFG +    + +LE
Sbjct:   69 DNVFWFIHVSDLHISRYANPTRTSDFERFCREVVPVIAPPVVVVTGDLTDAKDQYLIDSGQHQEEWQDYAAALDRSGVLDKVKWLDIRGNHDAFDV-AGLDHPSNLFARYSVSKNTSYTHVHQTTFGR--------YRFVAVDACPTPGPRRPFNFFGSLDVRAMTKLE 228          
BLAST of mRNA_M-pyrifera_M_contig99449.22876.1 vs. uniprot
Match: U9SN17_RHIID (Metallo-dependent phosphatase-like protein n=6 Tax=Rhizophagus TaxID=1129544 RepID=U9SN17_RHIID)

HSP 1 Score: 129 bits (323), Expect = 1.790e-31
Identity = 76/170 (44.71%), Postives = 99/170 (58.24%), Query Frame = 1
Query:    1 DDLTWFVHVSDLHLSKFNAVR-TERFELFLEEGLEVI-SPSFVLVTGDLVDAKHTDKVRSEQFPEEWATYKKLLSEHGWWD-PDKWIDLRGNHDCFGIGANWEQPQNLFREWSVTGSHFHGPVREFSFIRDGASDRPLVDVVTLDACPIVGAKTPLNFFGRMSTTQLDRL 501
            ++L +F+ ++DLH+SKF+    T  F  FL   L VI SP+FVLVTGDL DAK    V S+QF +EW TY+  L E G  + P  W DLRGNHDCF +G+ WE  QN+++++ V      G    F FI +   D      V +DACP  G   P NFFG   T  +DRL
Sbjct:   69 NNLFYFMQITDLHISKFHKTGGTSHFLHFLATVLPVIKSPAFVLVTGDLTDAKDEHFVTSQQFVDEWVTYQTALQESGVLNRPRFWHDLRGNHDCFNVGS-WESEQNMYKKYGV------GKREGFDFIVE--KDFGRYRFVGIDACPKAGVARPFNFFGYYETDDMDRL 229          
BLAST of mRNA_M-pyrifera_M_contig99449.22876.1 vs. uniprot
Match: A0A163B7A2_PHYB8 (Metallophos domain-containing protein n=1 Tax=Phycomyces blakesleeanus (strain ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC 33097 / NRRL 1555) TaxID=763407 RepID=A0A163B7A2_PHYB8)

HSP 1 Score: 128 bits (321), Expect = 3.940e-31
Identity = 70/171 (40.94%), Postives = 95/171 (55.56%), Query Frame = 1
Query:    4 DLTWFVHVSDLHLSKFNAV-RTERFELFLEEGLEVISPSFVLVTGDLVDAKHTDKVRSEQFPEEWATYKKLLSEHGWWDPDKWIDLRGNHDCFGIGANWEQPQNLFREWSVTGSHFHGPVREFSFIRDGASDRPLVDVVTLDACPIVGAKTPLNFFGRMSTTQLDRLEVEV 513
            ++ +F+ VSD+H+S+F     T+ F  FL+  L  I P FV+VTGDL DAK    V S Q  EEW  Y+K + E G   P  W DLRGNHDCF + A+W   QNL+R++  + S        +S+  +    +     +  D CP  G   P NFFG M +T +DRLE EV
Sbjct:   69 NIFYFIQVSDIHISRFKTKGHTQNFLHFLQSSLPTIKPEFVVVTGDLTDAKDPGGVNSYQSAEEWEIYEKAVKERGSDTP--WYDLRGNHDCFNM-ASWSSDQNLYRKYGKSASLVEQGQGVYSWSANKTFGQ--YKFIAADVCPKKGPARPFNFFGYMPSTTMDRLEAEV 234          
BLAST of mRNA_M-pyrifera_M_contig99449.22876.1 vs. uniprot
Match: A0A168MU16_ABSGL (Metallophos domain-containing protein n=1 Tax=Absidia glauca TaxID=4829 RepID=A0A168MU16_ABSGL)

HSP 1 Score: 125 bits (313), Expect = 1.590e-30
Identity = 69/167 (41.32%), Postives = 96/167 (57.49%), Query Frame = 1
Query:    4 DLTWFVHVSDLHLSKFNAVRTERFELFLEEGLEVISPSFVLVTGDLVDAKHTDKVRSEQFPEEWATYKKLLSEHGWWDPDKWIDLRGNHDCFGIGANWEQPQNLFREWSVTGSHFHGPVREFSFIRDGASDRPLVDVVTLDACPIVGAKTPLNFFGRMSTTQLDRLE 504
            +L +FV VSDLH+SKF + +T+ F  F+   L VI PSFV+VTGDL DAK TD +++ QF EEW  Y+  + E        W D+RGNHDCF +  +W+   NL+R +  +          +S+  +    +     V LDACP  G   PLNF+G + T  +DRLE
Sbjct:   82 NLFYFVQVSDLHISKFRS-QTDHFLHFVRSALPVIRPSFVVVTGDLTDAKDTDDIKTRQFKEEWQLYQSAVEEGS---DLAWYDMRGNHDCFDM-PSWQSDVNLYRTFGRSAKLLEQGQGVYSWHVNQTYGQ--YRFVALDACPKRGPARPLNFYGYLPTNTMDRLE 241          
BLAST of mRNA_M-pyrifera_M_contig99449.22876.1 vs. uniprot
Match: A0A8H4AHI7_GIGMA (Metallo-dependent phosphatase n=2 Tax=Gigaspora margarita TaxID=4874 RepID=A0A8H4AHI7_GIGMA)

HSP 1 Score: 125 bits (315), Expect = 2.130e-30
Identity = 71/169 (42.01%), Postives = 94/169 (55.62%), Query Frame = 1
Query:    1 DDLTWFVHVSDLHLSKF-NAVRTERFELFLEEGLEVISPSFVLVTGDLVDAKHTDKVRSEQFPEEWATYKKLLSEHGWWD-PDKWIDLRGNHDCFGIGANWEQPQNLFREWSVTGSHFHGPVREFSFIRDGASDRPLVDVVTLDACPIVGAKTPLNFFGRMSTTQLDRL 501
            +++ +F+ VSDLH+SK+        F  FL   L V+SPSFVLVTGDL D K     RS Q  +EW TY+  L E G  D P+ W D+RGNHDCF + ++W   QNL+R++SV         R F F+ +    R     + +DACP  G   P NFFG      +DRL
Sbjct:   61 ENILYFMQVSDLHISKYYKPGGASHFLYFLSTALPVVSPSFVLVTGDLTDGKEQYYGRSNQIIDEWLTYQTALQESGVLDRPNFWYDMRGNHDCFDM-SSWNSEQNLYRQYSVVKK------RGFDFMVEKTFGR--YRFIGIDACPKSGPSRPYNFFGYYEINDMDRL 220          
BLAST of mRNA_M-pyrifera_M_contig99449.22876.1 vs. uniprot
Match: A0A397T043_9GLOM (Metallo-dependent phosphatase-like protein n=1 Tax=Glomus cerebriforme TaxID=658196 RepID=A0A397T043_9GLOM)

HSP 1 Score: 125 bits (315), Expect = 2.200e-30
Identity = 75/170 (44.12%), Postives = 96/170 (56.47%), Query Frame = 1
Query:    1 DDLTWFVHVSDLHLSKFNAVR-TERFELFLEEGLEVI-SPSFVLVTGDLVDAKHTDKVRSEQFPEEWATYKKLLSEHGWWD-PDKWIDLRGNHDCFGIGANWEQPQNLFREWSVTGSHFHGPVREFSFIRDGASDRPLVDVVTLDACPIVGAKTPLNFFGRMSTTQLDRL 501
            D+L +F+ +SDLH+SKF     T  F  FL   L VI SP+FVLVTGDL DAK    V S QF +EW TY+  L E G  + P  W DLRGNHDCF +G+ W   QN+++++ V         + F F+ +   D      V +DACP  G   P NFFG   T  +DRL
Sbjct:   70 DNLLYFMQISDLHISKFQKTGGTSHFLHFLATVLPVIRSPAFVLVTGDLTDAKDEHFVTSRQFIDEWVTYQTALQESGALNRPGFWHDLRGNHDCFNVGS-WGSEQNMYKKYGVEKQ------QGFDFMVE--KDFGRYRFVGIDACPKAGVARPFNFFGYYETDDMDRL 230          
BLAST of mRNA_M-pyrifera_M_contig99449.22876.1 vs. uniprot
Match: A0A1Y2GRH7_9FUNG (Metallophos domain-containing protein n=1 Tax=Lobosporangium transversale TaxID=64571 RepID=A0A1Y2GRH7_9FUNG)

HSP 1 Score: 125 bits (314), Expect = 3.550e-30
Identity = 67/171 (39.18%), Postives = 92/171 (53.80%), Query Frame = 1
Query:    1 DDLTWFVHVSDLHLSKFNAVRTERFELFLEEGLEVISPSFVLVTGDLVDAKHTDKVRSEQFPEEWATYKKLLSEHGWWDPDK---WIDLRGNHDCFGIGANWEQPQNLFREWSVTGSHFHGPVREFSFIRDGASDRPLVDVVTLDACPIVGAKTPLNFFGRMSTTQLDRLE 504
            D++ +FV VSDLH+S+F    T  F  FL   L ++ P+FV+VTGDL DAK    V S Q+ +EW TY+  L + G  +      W DLRGNHDCF +  +W+  QN+F + S T        R   F+ D  +       + +DACP  G   P NFFG   T  +D L+
Sbjct:  100 DNIFYFVQVSDLHISRFRKASTTNFYTFLSTALPLVEPAFVVVTGDLTDAKDKHLVGSMQYMDEWITYRDALEQSGVLNKRNGTFWHDLRGNHDCFDV-PSWDSKQNMFADMSAT--------RAPGFMFDVKTGYGKYGFIGIDACPKPGPARPYNFFGLFETPDMDNLK 261          
BLAST of mRNA_M-pyrifera_M_contig99449.22876.1 vs. uniprot
Match: A0A086TIZ8_9FUNG (Metallophos domain-containing protein n=1 Tax=Podila verticillata NRRL 6337 TaxID=1069443 RepID=A0A086TIZ8_9FUNG)

HSP 1 Score: 124 bits (311), Expect = 9.000e-30
Identity = 66/170 (38.82%), Postives = 90/170 (52.94%), Query Frame = 1
Query:    1 DDLTWFVHVSDLHLSKFNAVRTERFELFLEEGLEVISPSFVLVTGDLVDAKHTDKVRSEQFPEEWATYKKLLSEHGWWDPDK---WIDLRGNHDCFGIGANWEQPQNLFREWSVTGSHFHGPVREFSFIRDGASDRPLVDVVTLDACPIVGAKTPLNFFGRMSTTQLDRL 501
            D++ +FV VSDLH+S F    T  F  FL   L ++ P+FV+VTGDL DAK    V S Q+ +EW TYK  L E G         W DLRGNHDCF +  +W+  +N++ + S T +          F+ D  ++      + +DACP  G   P NFFG   T  +D L
Sbjct:   85 DNIFYFVQVSDLHISTFRKASTANFYTFLSTALPLVDPAFVVVTGDLTDAKDQQLVGSMQYVDEWVTYKNALEESGVLKKRNGTFWHDLRGNHDCFNV-PDWDSKENMYADMSATKTP--------GFMFDVKTEYGKYGFIGIDACPKPGPARPYNFFGLFETPDMDSL 245          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig99449.22876.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2H9TPG7_9FUNG8.840e-3441.04Transitional endoplasmic reticulum ATPase TER94-li... [more]
A0A397H154_9GLOM7.890e-3343.27Metallophos domain-containing protein n=1 Tax=Dive... [more]
A0A0D2URX7_CAPO32.350e-3241.42Metallophos domain-containing protein n=1 Tax=Caps... [more]
U9SN17_RHIID1.790e-3144.71Metallo-dependent phosphatase-like protein n=6 Tax... [more]
A0A163B7A2_PHYB83.940e-3140.94Metallophos domain-containing protein n=1 Tax=Phyc... [more]
A0A168MU16_ABSGL1.590e-3041.32Metallophos domain-containing protein n=1 Tax=Absi... [more]
A0A8H4AHI7_GIGMA2.130e-3042.01Metallo-dependent phosphatase n=2 Tax=Gigaspora ma... [more]
A0A397T043_9GLOM2.200e-3044.12Metallo-dependent phosphatase-like protein n=1 Tax... [more]
A0A1Y2GRH7_9FUNG3.550e-3039.18Metallophos domain-containing protein n=1 Tax=Lobo... [more]
A0A086TIZ8_9FUNG9.000e-3038.82Metallophos domain-containing protein n=1 Tax=Podi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig99449contigM-pyrifera_M_contig99449:10..522 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeOpisthokonta
Seed ortholog score133.7
Seed ortholog evalue8.4e-29
Seed eggNOG ortholog192875.XP_004342899.1
Preferred nameTMEM62
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Calcineurin-like phosphoesterase
EggNOG OGs39MBT@33154,COG0571@1,KOG0701@2759
COG Functional cat.A
Best tax levelOpisthokonta
Best eggNOG OGNA|NA|NA
Exons1
Model size513
Cds size513
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816912.0744011-CDS-M-pyrifera_M_contig99449:9..5221622816912.0744011-CDS-M-pyrifera_M_contig99449:9..522Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99449 10..522 -
1692278251.3643792-CDS-M-pyrifera_M_contig99449:9..5221692278251.3643792-CDS-M-pyrifera_M_contig99449:9..522Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99449 10..522 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig99449.22876.1prot_M-pyrifera_M_contig99449.22876.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig99449 10..522 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig99449.22876.1

>prot_M-pyrifera_M_contig99449.22876.1 ID=prot_M-pyrifera_M_contig99449.22876.1|Name=mRNA_M-pyrifera_M_contig99449.22876.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=171bp
DDLTWFVHVSDLHLSKFNAVRTERFELFLEEGLEVISPSFVLVTGDLVDA
KHTDKVRSEQFPEEWATYKKLLSEHGWWDPDKWIDLRGNHDCFGIGANWE
QPQNLFREWSVTGSHFHGPVREFSFIRDGASDRPLVDVVTLDACPIVGAK
TPLNFFGRMSTTQLDRLEVEV
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mRNA from alignment at M-pyrifera_M_contig99449:10..522-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig99449.22876.1 ID=mRNA_M-pyrifera_M_contig99449.22876.1|Name=mRNA_M-pyrifera_M_contig99449.22876.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=513bp|location=Sequence derived from alignment at M-pyrifera_M_contig99449:10..522- (Macrocystis pyrifera P11B4 male)
GATGACCTGACATGGTTTGTGCATGTCAGTGACTTGCACCTCAGCAAATT CAACGCTGTGAGGACAGAGAGATTCGAGCTATTCCTGGAGGAGGGCCTGG AGGTTATTAGCCCCTCATTCGTTCTGGTGACGGGAGACCTCGTGGATGCC AAGCACACAGACAAAGTAAGGTCTGAACAGTTTCCTGAGGAGTGGGCAAC GTACAAGAAGCTTCTCTCGGAGCATGGGTGGTGGGACCCTGACAAATGGA TTGATCTGAGGGGAAATCACGACTGTTTCGGCATTGGAGCGAATTGGGAA CAGCCCCAGAACCTTTTCAGAGAGTGGAGTGTCACTGGAAGCCACTTCCA CGGGCCTGTGCGCGAGTTTTCGTTCATAAGGGACGGGGCAAGTGACCGGC CACTCGTTGACGTCGTCACTCTCGACGCGTGTCCTATTGTTGGCGCGAAA ACGCCGTTGAACTTCTTTGGAAGAATGTCAACCACTCAGCTGGATCGATT GGAGGTAGAGGTG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig99449:10..522-

>mRNA_M-pyrifera_M_contig99449.22876.1 ID=mRNA_M-pyrifera_M_contig99449.22876.1|Name=mRNA_M-pyrifera_M_contig99449.22876.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1026bp|location=Sequence derived from alignment at M-pyrifera_M_contig99449:10..522- (Macrocystis pyrifera P11B4 male)
GATGACCTGACATGGTTTGTGCATGTCAGTGACTTGCACCTCAGCAAATT
CAACGCTGTGAGGACAGAGAGATTCGAGCTATTCCTGGAGGAGGGCCTGG
AGGTTATTAGCCCCTCATTCGTTCTGGTGACGGGAGACCTCGTGGATGCC
AAGCACACAGACAAAGTAAGGTCTGAACAGTTTCCTGAGGAGTGGGCAAC
GTACAAGAAGCTTCTCTCGGAGCATGGGTGGTGGGACCCTGACAAATGGA
TTGATCTGAGGGGAAATCACGACTGTTTCGGCATTGGAGCGAATTGGGAA
CAGCCCCAGAACCTTTTCAGAGAGTGGAGTGTCACTGGAAGCCACTTCCA
CGGGCCTGTGCGCGAGTTTTCGTTCATAAGGGACGGGGCAAGTGACCGGC
CACTCGTTGACGTCGTCACTCTCGACGCGTGTCCTATTGTTGGCGCGAAA
ACGCCGTTGAACTTCTTTGGAAGAATGTCAACCACTCAGCTGGATCGATT
GGAGGTAGAGGTGGATGACCTGACATGGTTTGTGCATGTCAGTGACTTGC
ACCTCAGCAAATTCAACGCTGTGAGGACAGAGAGATTCGAGCTATTCCTG
GAGGAGGGCCTGGAGGTTATTAGCCCCTCATTCGTTCTGGTGACGGGAGA
CCTCGTGGATGCCAAGCACACAGACAAAGTAAGGTCTGAACAGTTTCCTG
AGGAGTGGGCAACGTACAAGAAGCTTCTCTCGGAGCATGGGTGGTGGGAC
CCTGACAAATGGATTGATCTGAGGGGAAATCACGACTGTTTCGGCATTGG
AGCGAATTGGGAACAGCCCCAGAACCTTTTCAGAGAGTGGAGTGTCACTG
GAAGCCACTTCCACGGGCCTGTGCGCGAGTTTTCGTTCATAAGGGACGGG
GCAAGTGACCGGCCACTCGTTGACGTCGTCACTCTCGACGCGTGTCCTAT
TGTTGGCGCGAAAACGCCGTTGAACTTCTTTGGAAGAATGTCAACCACTC
AGCTGGATCGATTGGAGGTAGAGGTG
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