mRNA_M-pyrifera_M_contig99355.22856.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig99355.22856.1
Unique NamemRNA_M-pyrifera_M_contig99355.22856.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig99355.22856.1 vs. uniprot
Match: K3X3H5_GLOUD (DNA-directed RNA polymerases I, II, and III subunit RPABC3 n=29 Tax=Oomycota TaxID=4762 RepID=K3X3H5_GLOUD)

HSP 1 Score: 129 bits (325), Expect = 2.520e-36
Identity = 61/111 (54.95%), Postives = 79/111 (71.17%), Query Frame = 1 
Query:    1 KGESTRTDLILDVNSEVYPVMEGERLHIVLARTLHEDGTPDDGKFDQSGKPTLLDRFEYGMYGKVFKYDDIGDGNISVYASFGGLLMNLRGHQSGISDVTLDARIYCLIKK 333
            KG S   +L LD+NSEVY + + E+    L  TL  +G PDDG ++QSGKPTLLDR++YGMYGKVF+YD  G   +++YASFGGLLM LRG Q  +  +  D RIYCL++K
Sbjct:   31 KGVSYELELTLDINSEVYRLRQNEKFTFALVSTLDLEGKPDDGTYNQSGKPTLLDRYDYGMYGKVFQYDHEGGNVVAIYASFGGLLMCLRGEQRHLHMIHNDTRIYCLLRK 141
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BLAST of mRNA_M-pyrifera_M_contig99355.22856.1 vs. uniprot
Match: A0A024TMY4_9STRA (DNA-directed RNA polymerases I, II, and III subunit RPABC3 n=9 Tax=Saprolegniaceae TaxID=4764 RepID=A0A024TMY4_9STRA)

HSP 1 Score: 129 bits (323), Expect = 5.230e-36
Identity = 59/111 (53.15%), Postives = 82/111 (73.87%), Query Frame = 1 
Query:    1 KGESTRTDLILDVNSEVYPVMEGERLHIVLARTLHEDGTPDDGKFDQSGKPTLLDRFEYGMYGKVFKYDDIGDGNISVYASFGGLLMNLRGHQSGISDVTLDARIYCLIKK 333
            KG S   +L+LD+NSEV+P+   E+    L  TL  +G PDDG FDQ+GKPTLLDR++YGMYGKVF+YD  G   +++YASFGGLLM LRG Q+ + ++   +R+YCL+++
Sbjct:   32 KGVSYELELMLDINSEVFPMKVHEQFTFALVSTLDLEGKPDDGIFDQTGKPTLLDRYDYGMYGKVFQYDHEGGNMVAIYASFGGLLMCLRGDQAHLRNIHNGSRVYCLMRR 142
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BLAST of mRNA_M-pyrifera_M_contig99355.22856.1 vs. uniprot
Match: A0A2R5G8T6_9STRA (DNA-directed RNA polymerases I, II, and III subunit RPABC3 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5G8T6_9STRA)

HSP 1 Score: 127 bits (319), Expect = 3.190e-35
Identity = 56/114 (49.12%), Postives = 84/114 (73.68%), Query Frame = 1 
Query:    1 KGESTRTDLILDVNSEVYPVMEGERLHIVLARTLHEDGTPDDGKFDQSGKPTLLDRFEYGMYGKVFKYDDIGDGN--ISVYASFGGLLMNLRGHQSGISDVTLDARIYCLIKKA 336
            +G +   DL++D+ SE++P+   ER  + LA TL  DG PDDG ++QSG PTLLD+F+YGM+G+VF Y+ +G+G   +++YAS+GGLLM L G Q  ++ + LD RIYCL+K++
Sbjct:   40 RGATFEMDLVVDIQSELFPMAMNERFTLALASTLDMDGKPDDGTYNQSGAPTLLDKFDYGMHGRVFSYEYVGEGEHRVAIYASYGGLLMKLVGDQRKLNTIDLDTRIYCLLKRS 153
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BLAST of mRNA_M-pyrifera_M_contig99355.22856.1 vs. uniprot
Match: A0A024G723_9STRA (Uncharacterized protein n=2 Tax=Albugo candida TaxID=65357 RepID=A0A024G723_9STRA)

HSP 1 Score: 125 bits (314), Expect = 3.330e-34
Identity = 58/111 (52.25%), Postives = 80/111 (72.07%), Query Frame = 1 
Query:    1 KGESTRTDLILDVNSEVYPVMEGERLHIVLARTLHEDGTPDDGKFDQSGKPTLLDRFEYGMYGKVFKYDDIGDGNISVYASFGGLLMNLRGHQSGISDVTLDARIYCLIKK 333
            KG S   +L LD+NSEV+ + + ++    L  TL  +G PDDG ++QSGK TLLDR++YGMYGK+F+Y+  G  N+++YASFGGLLM LRG Q  +  V  D+RIYCL++K
Sbjct:   67 KGVSYELELTLDINSEVWRLKKNDKFTFALVSTLDLEGKPDDGIYNQSGKATLLDRYDYGMYGKIFQYEHEGGNNVAIYASFGGLLMRLRGEQRHLHLVHNDSRIYCLLRK 177
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BLAST of mRNA_M-pyrifera_M_contig99355.22856.1 vs. uniprot
Match: A0A0W8DEU7_PHYNI (DNA-directed RNA polymerases I, II, and III subunit RPABC3 n=1 Tax=Phytophthora nicotianae TaxID=4790 RepID=A0A0W8DEU7_PHYNI)

HSP 1 Score: 123 bits (309), Expect = 4.100e-34
Identity = 57/100 (57.00%), Postives = 74/100 (74.00%), Query Frame = 1 
Query:   34 DVNSEVYPVMEGERLHIVLARTLHEDGTPDDGKFDQSGKPTLLDRFEYGMYGKVFKYDDIGDGNISVYASFGGLLMNLRGHQSGISDVTLDARIYCLIKK 333
            D+NSEVY + + E+  + L  TL  +G PDDG F+QSGKPTLLDR++YGMYGKVF+YD  G   +++YASFGGLLM LRG Q  +  +  D RIYCL++K
Sbjct:   25 DINSEVYRLRQNEKFTLALVSTLDLEGKPDDGIFNQSGKPTLLDRYDYGMYGKVFQYDHEGGNMVAIYASFGGLLMCLRGEQRHLHMIHNDTRIYCLLRK 124
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BLAST of mRNA_M-pyrifera_M_contig99355.22856.1 vs. uniprot
Match: A0A7S1BC37_9STRA (DNA-directed RNA polymerases I, II, and III subunit RPABC3 n=1 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1BC37_9STRA)

HSP 1 Score: 124 bits (310), Expect = 5.060e-34
Identity = 59/110 (53.64%), Postives = 79/110 (71.82%), Query Frame = 1 
Query:    4 GESTRTDLILDVNSEVYPVMEGERLHIVLARTLHEDGTPDDGKFDQSGKPTLLDRFEYGMYGKVFKYDDIGDGNISVYASFGGLLMNLRGHQSGISDVTLDARIYCLIKK 333
            G + + DL+LD NSE+YP+ E E++ IVL+ TL  DG PDDG++DQSG+PT+ D +EY M+G+VF     GDG I+V+ SFGGLLM L G Q  + +V  D R+Y LIKK
Sbjct:   34 GTTFQCDLLLDTNSEIYPIAEEEKITIVLSSTLSLDGRPDDGEYDQSGEPTMADNYEYVMHGRVFDIKYKGDGVIAVHVSFGGLLMRLTGDQRHLENVRADERLYILIKK 143
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BLAST of mRNA_M-pyrifera_M_contig99355.22856.1 vs. uniprot
Match: F0W6P2_9STRA (DNAdirected RNA polymerase I putative n=3 Tax=Albugo laibachii Nc14 TaxID=890382 RepID=F0W6P2_9STRA)

HSP 1 Score: 123 bits (309), Expect = 6.710e-33
Identity = 57/111 (51.35%), Postives = 79/111 (71.17%), Query Frame = 1 
Query:    1 KGESTRTDLILDVNSEVYPVMEGERLHIVLARTLHEDGTPDDGKFDQSGKPTLLDRFEYGMYGKVFKYDDIGDGNISVYASFGGLLMNLRGHQSGISDVTLDARIYCLIKK 333
            KG S   +L LD+NSEV+ + + ++    L  TL  +G PDDG ++QSGK TLLDR++YGMYGK+F Y+  G  N+++YASFGGLLM LRG Q  +  V  D+R+YCL++K
Sbjct:  114 KGVSYELELTLDINSEVWRLKKNDKFTFALVCTLDLEGKPDDGIYNQSGKATLLDRYDYGMYGKIFHYEHEGGNNVAIYASFGGLLMRLRGEQRHLHLVHNDSRVYCLLRK 224
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BLAST of mRNA_M-pyrifera_M_contig99355.22856.1 vs. uniprot
Match: A0A813B0I3_9DINO (DNA-directed RNA polymerases I, II, and III subunit RPABC3 n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813B0I3_9DINO)

HSP 1 Score: 120 bits (301), Expect = 1.520e-32
Identity = 55/112 (49.11%), Postives = 78/112 (69.64%), Query Frame = 1 
Query:    1 KGESTRTDLILDVNSEVYPVMEGERLHIVLARTLHEDGTPDDGKFDQSGKPTLLDRFEYGMYGKVFKYDDIGDGNISVYASFGGLLMNLRGHQSGISDVTLDARIYCLIKKA 336
            K E+   D+ +D+ SEVYP+ + E+  +VL  TL  DG PDDG +DQS K + LD FEYGM GK+FKYD   D  +S+YAS+GGLLM ++G    +  + LD+R+YCL+++A
Sbjct:   37 KSETYGIDMSIDIASEVYPLKKDEKFAMVLRSTLRLDGKPDDGTYDQSTKESALDAFEYGMSGKIFKYDHAEDARVSIYASYGGLLMLVQGKAEHLKALKLDSRVYCLMRRA 148
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BLAST of mRNA_M-pyrifera_M_contig99355.22856.1 vs. uniprot
Match: A0A7S1CMR6_9STRA (Hypothetical protein n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CMR6_9STRA)

HSP 1 Score: 120 bits (301), Expect = 1.970e-32
Identity = 59/115 (51.30%), Postives = 81/115 (70.43%), Query Frame = 1 
Query:    1 KGESTRTDLILDVNSEVYPVMEGERLHIVLARTLHEDGTPDDGKFDQS-GKPTLLDRFEYGMYGKVFKYDDIGDGNISVYASFGGLLMNLRGHQSGISDVTLDARIYCLIKKALA 342
            KGE+    L +DV SEV+PV  GE+L + +  TL  DG PDDG +  + G+ +LLD+++YGM+G+VFKYD   D  +SVYASFGGLLM L G Q  +S ++LD R+YCLI++  A
Sbjct:   34 KGETWDVLLKIDVASEVFPVKAGEKLAVAVVSTLRLDGEPDDGTYQHNEGEESLLDQYDYGMHGRVFKYDYEEDDRVSVYASFGGLLMLLHGQQDSLSSLSLDQRVYCLIRRPKA 148
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BLAST of mRNA_M-pyrifera_M_contig99355.22856.1 vs. uniprot
Match: A0A2R6WCZ2_MARPO (Uncharacterized protein n=2 Tax=Marchantia polymorpha TaxID=3197 RepID=A0A2R6WCZ2_MARPO)

HSP 1 Score: 113 bits (283), Expect = 6.290e-30
Identity = 55/107 (51.40%), Postives = 75/107 (70.09%), Query Frame = 1 
Query:   22 DLILDVNSEVYPVMEGERLHIVLARTLHEDGTPDDGKFDQSGKPTLLDRFEYGMYGKVFKYDDI---GDGNISVYASFGGLLMNLRGHQSGISDVTLDARIYCLIKK 333
            DL+ DVN++VYP+   E+  + LA TL  DGTPDDG FDQSG+ +L D+FEY MYGK++KY D+   G   + VY SFGGLLM L+G  + ++   +D R+Y LI+K
Sbjct:   37 DLLFDVNTDVYPMALEEKFALALATTLSLDGTPDDGFFDQSGRKSLADKFEYVMYGKLYKYSDVDVNGISKVEVYISFGGLLMMLKGDPNHLNAFQVDQRLYLLIRK 143
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig99355.22856.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
K3X3H5_GLOUD2.520e-3654.95DNA-directed RNA polymerases I, II, and III subuni... [more]
A0A024TMY4_9STRA5.230e-3653.15DNA-directed RNA polymerases I, II, and III subuni... [more]
A0A2R5G8T6_9STRA3.190e-3549.12DNA-directed RNA polymerases I, II, and III subuni... [more]
A0A024G723_9STRA3.330e-3452.25Uncharacterized protein n=2 Tax=Albugo candida Tax... [more]
A0A0W8DEU7_PHYNI4.100e-3457.00DNA-directed RNA polymerases I, II, and III subuni... [more]
A0A7S1BC37_9STRA5.060e-3453.64DNA-directed RNA polymerases I, II, and III subuni... [more]
F0W6P2_9STRA6.710e-3351.35DNAdirected RNA polymerase I putative n=3 Tax=Albu... [more]
A0A813B0I3_9DINO1.520e-3249.11DNA-directed RNA polymerases I, II, and III subuni... [more]
A0A7S1CMR6_9STRA1.970e-3251.30Hypothetical protein n=1 Tax=Bicosoecida sp. CB-20... [more]
A0A2R6WCZ2_MARPO6.290e-3051.40Uncharacterized protein n=2 Tax=Marchantia polymor... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig99355contigM-pyrifera_M_contig99355:3..470 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score134.0
Seed ortholog evalue4.4e-29
Seed eggNOG ortholog164328.Phyra94262
Preferred nameRPB8
KEGG rclassRC02795
KEGG koko:K03016
KEGG ReactionR00435,R00441,R00442,R00443
KEGG Pathwayko00230,ko00240,ko01100,ko03020,ko04623,ko05016,ko05169,map00230,map00240,map01100,map03020,map04623,map05016,map05169
KEGG ModuleM00180,M00181,M00182
Hectar predicted targeting categoryother localisation
GOsGO:0000375,GO:0000377,GO:0000398,GO:0000418,GO:0000419,GO:0000428,GO:0003674,GO:0003676,GO:0003677,GO:0003697,GO:0003824,GO:0003899,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005665,GO:0005666,GO:0005730,GO:0005736,GO:0005737,GO:0005829,GO:0006139,GO:0006259,GO:0006281,GO:0006283,GO:0006289,GO:0006351,GO:0006352,GO:0006353,GO:0006354,GO:0006360,GO:0006361,GO:0006363,GO:0006366,GO:0006367,GO:0006368,GO:0006370,GO:0006383,GO:0006396,GO:0006397,GO:0006399,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0007154,GO:0007165,GO:0007166,GO:0007167,GO:0007169,GO:0008150,GO:0008152,GO:0008380,GO:0008543,GO:0009058,GO:0009059,GO:0009301,GO:0009304,GO:0009452,GO:0009719,GO:0009893,GO:0009987,GO:0010033,GO:0010467,GO:0010468,GO:0010604,GO:0010628,GO:0016070,GO:0016071,GO:0016073,GO:0016591,GO:0016740,GO:0016772,GO:0016779,GO:0018130,GO:0019222,GO:0019438,GO:0023052,GO:0030880,GO:0031974,GO:0031981,GO:0032774,GO:0032991,GO:0032993,GO:0033554,GO:0034062,GO:0034641,GO:0034645,GO:0034654,GO:0034660,GO:0036260,GO:0040029,GO:0042221,GO:0042795,GO:0042797,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044344,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044452,GO:0044464,GO:0045815,GO:0046483,GO:0048518,GO:0050789,GO:0050794,GO:0050896,GO:0051716,GO:0055029,GO:0060147,GO:0060255,GO:0060964,GO:0060966,GO:0060968,GO:0061695,GO:0065007,GO:0070013,GO:0070848,GO:0070887,GO:0071310,GO:0071363,GO:0071495,GO:0071704,GO:0071774,GO:0090304,GO:0097159,GO:0097659,GO:0097747,GO:0098781,GO:0140098,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1902494,GO:1990234
EggNOG free text desc.RNA polymerase Rpb8
EggNOG OGs3Q7SS@4776,KOG3400@1,KOG3400@2759
COG Functional cat.K
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
BRITEbr01611,ko00000,ko00001,ko00002,ko03021,ko03400
Exons3
Model size348
Cds size348
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig99355.22856.1prot_M-pyrifera_M_contig99355.22856.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig99355 3..470 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816911.076526-CDS-M-pyrifera_M_contig99355:2..1521622816911.076526-CDS-M-pyrifera_M_contig99355:2..152Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99355 3..152 +
1692278250.7931716-CDS-M-pyrifera_M_contig99355:2..1521692278250.7931716-CDS-M-pyrifera_M_contig99355:2..152Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99355 3..152 +
1622816911.087844-CDS-M-pyrifera_M_contig99355:210..2871622816911.087844-CDS-M-pyrifera_M_contig99355:210..287Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99355 211..287 +
1692278250.8179169-CDS-M-pyrifera_M_contig99355:210..2871692278250.8179169-CDS-M-pyrifera_M_contig99355:210..287Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99355 211..287 +
1622816911.0980942-CDS-M-pyrifera_M_contig99355:349..4701622816911.0980942-CDS-M-pyrifera_M_contig99355:349..470Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99355 350..470 +
1692278250.8374164-CDS-M-pyrifera_M_contig99355:349..4701692278250.8374164-CDS-M-pyrifera_M_contig99355:349..470Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99355 350..470 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig99355.22856.1

>prot_M-pyrifera_M_contig99355.22856.1 ID=prot_M-pyrifera_M_contig99355.22856.1|Name=mRNA_M-pyrifera_M_contig99355.22856.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=116bp
KGESTRTDLILDVNSEVYPVMEGERLHIVLARTLHEDGTPDDGKFDQSGK
PTLLDRFEYGMYGKVFKYDDIGDGNISVYASFGGLLMNLRGHQSGISDVT
LDARIYCLIKKALAL*
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mRNA from alignment at M-pyrifera_M_contig99355:3..470+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig99355.22856.1 ID=mRNA_M-pyrifera_M_contig99355.22856.1|Name=mRNA_M-pyrifera_M_contig99355.22856.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=468bp|location=Sequence derived from alignment at M-pyrifera_M_contig99355:3..470+ (Macrocystis pyrifera P11B4 male)
AAGGGCGAGTCGACGCGGACGGACCTGATTCTGGACGTGAACTCGGAGGT
GTACCCGGTGATGGAGGGGGAGCGGCTGCACATCGTGCTGGCCAGGACGC
TTCACGAGGATGGTACGCCTGATGACGGCAAGTTTGACCAGTCGGGCAAG
GTGAGCGGCGGCGAATGCGCGGTTTCGCTGGCGCGACACTGACGCTTGAC
TTGCACAGCCGACGCTGCTGGACAGGTTCGAGTACGGCATGTACGGGAAG
GTGTTCAAGTACGACGACATTGGCGATGGCAACATGTGCGTGGCTGGGCG
CTTGGGGGTGCGGGTGGGTCTGCATCTCACGCGTGTCTCGTGCGCAGCTC
CGTGTACGCGTCCTTCGGAGGCTTGCTGATGAACCTGCGGGGCCACCAGA
GCGGCATTTCGGATGTGACGCTGGACGCCAGAATATATTGCCTCATCAAG
AAGGCGCTGGCTCTTTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig99355:3..470+

>mRNA_M-pyrifera_M_contig99355.22856.1 ID=mRNA_M-pyrifera_M_contig99355.22856.1|Name=mRNA_M-pyrifera_M_contig99355.22856.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=696bp|location=Sequence derived from alignment at M-pyrifera_M_contig99355:3..470+ (Macrocystis pyrifera P11B4 male)
AAGGGCGAGTCGACGCGGACGGACCTGATTCTGGACGTGAACTCGGAGGT
GTACCCGGTGATGGAGGGGGAGCGGCTGCACATCGTGCTGGCCAGGACGC
TTCACGAGGATGGTACGCCTGATGACGGCAAGTTTGACCAGTCGGGCAAG
AAGGGCGAGTCGACGCGGACGGACCTGATTCTGGACGTGAACTCGGAGGT
GTACCCGGTGATGGAGGGGGAGCGGCTGCACATCGTGCTGGCCAGGACGC
TTCACGAGGATGGTACGCCTGATGACGGCAAGTTTGACCAGTCGGGCAAG
CCGACGCTGCTGGACAGGTTCGAGTACGGCATGTACGGGAAGGTGTTCAA
GTACGACGACATTGGCGATGGCAACATCCGACGCTGCTGGACAGGTTCGA
GTACGGCATGTACGGGAAGGTGTTCAAGTACGACGACATTGGCGATGGCA
ACATCTCCGTGTACGCGTCCTTCGGAGGCTTGCTGATGAACCTGCGGGGC
CACCAGAGCGGCATTTCGGATGTGACGCTGGACGCCAGAATATATTGCCT
CATCAAGAAGGCGCTGGCTCTTTAGCTCCGTGTACGCGTCCTTCGGAGGC
TTGCTGATGAACCTGCGGGGCCACCAGAGCGGCATTTCGGATGTGACGCT
GGACGCCAGAATATATTGCCTCATCAAGAAGGCGCTGGCTCTTTAG
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