mRNA_M-pyrifera_M_contig99271.22839.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig99271.22839.1
Unique NamemRNA_M-pyrifera_M_contig99271.22839.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig99271.22839.1 vs. uniprot
Match: UPI000C051E56 (mitotic checkpoint serine/threonine-protein kinase BUB1 beta-like n=1 Tax=Stylophora pistillata TaxID=50429 RepID=UPI000C051E56)

HSP 1 Score: 99.8 bits (247), Expect = 3.740e-22
Identity = 43/104 (41.35%), Postives = 72/104 (69.23%), Query Frame = 1
Query:    1 MERCTAAFRVDARYKQDLRFLRIWLLYADICSNAVDVFNYLLHKEIGTEWELLYSCKAAVLENLGKYKEAEEALKLGVNREAKPLDKMENNHKRFLERMALRVA 312
            +ERC   F+ + RYK D R++++WL Y DIC++ +++FNY+  +++G    L Y   A +LENLG + +A+E L LG++R A+PL++++  HK F  R+A RV+
Sbjct:   91 LERCMTTFKDEERYKNDERYVKVWLRYTDICTDPLEIFNYMHSQQLGNGLALFYEAWALILENLGDHNKADEVLNLGIHRSAQPLERLQKQHKAFELRLAERVS 194          
BLAST of mRNA_M-pyrifera_M_contig99271.22839.1 vs. uniprot
Match: A0A2B4RTS4_STYPI (Mitotic checkpoint serine/threonine-protein kinase BUB1 beta n=2 Tax=Stylophora pistillata TaxID=50429 RepID=A0A2B4RTS4_STYPI)

HSP 1 Score: 99.8 bits (247), Expect = 1.970e-21
Identity = 43/104 (41.35%), Postives = 72/104 (69.23%), Query Frame = 1
Query:    1 MERCTAAFRVDARYKQDLRFLRIWLLYADICSNAVDVFNYLLHKEIGTEWELLYSCKAAVLENLGKYKEAEEALKLGVNREAKPLDKMENNHKRFLERMALRVA 312
            +ERC   F+ + RYK D R++++WL Y DIC++ +++FNY+  +++G    L Y   A +LENLG + +A+E L LG++R A+PL++++  HK F  R+A RV+
Sbjct:   91 LERCMTTFKDEERYKNDERYVKVWLRYTDICTDPLEIFNYMHSQQLGNGLALFYEAWALILENLGDHNKADEVLNLGIHRSAQPLERLQKQHKAFELRLAERVS 194          
BLAST of mRNA_M-pyrifera_M_contig99271.22839.1 vs. uniprot
Match: A0A8J4PY03_9MYCE (Uncharacterized protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4PY03_9MYCE)

HSP 1 Score: 99.0 bits (245), Expect = 3.640e-21
Identity = 46/103 (44.66%), Postives = 68/103 (66.02%), Query Frame = 1
Query:    1 MERCTAAFRVDARYKQDLRFLRIWLLYADICSNAVDVFNYLLHKEIGTEWELLYSCKAAVLENLGKYKEAEEALKLGVNREAKPLDKMENNHKRFLERMALRV 309
            +ERCT  F    +YK D R+LRIW+ YAD+C + V++F +L ++ IG+   LLY  KA V EN G Y+ A+EA KLG+ R+A PL++++  H  F  R+  R+
Sbjct:   93 LERCTKLFYSYEKYKNDHRYLRIWITYADMCRDPVEIFAFLENRGIGSSLSLLYEAKAIVFENKGNYQAADEAFKLGIQRKASPLERLQQKHLDFERRLIARI 195          
BLAST of mRNA_M-pyrifera_M_contig99271.22839.1 vs. uniprot
Match: D3AZV9_POLPP (Uncharacterized protein n=1 Tax=Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) TaxID=670386 RepID=D3AZV9_POLPP)

HSP 1 Score: 96.3 bits (238), Expect = 3.160e-20
Identity = 46/123 (37.40%), Postives = 80/123 (65.04%), Query Frame = 1
Query:    1 MERCTAAFRVDARYKQDLRFLRIWLLYADICSNAVDVFNYLLHKEIGTEWELLYSCKAAVLENLGKYKEAEEALKLGVNREAKPLDKMENNHKRFLERMALRVAK-AKQAKADRELVKGSADS 366
            +ERCT+ F  + +Y+ D R++++W++YAD+C + +++FNYL   +IG    L Y  KA + EN   Y+ A+++ +LG+ R+A+PLD+++  H  F  RM + ++K AK+      L  G+ADS
Sbjct:   87 LERCTSMFVNNEKYRNDQRYMKLWIIYADMCRDPIEIFNYLETNKIGLTLSLFYEAKAIIYENKSNYQMADQSYQLGLKRQAQPLDRLKKRHDDFQRRMVVAMSKSAKEGGIIPSLSVGAADS 209          
BLAST of mRNA_M-pyrifera_M_contig99271.22839.1 vs. uniprot
Match: UPI000644EA2D (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644EA2D)

HSP 1 Score: 93.2 bits (230), Expect = 3.760e-19
Identity = 40/106 (37.74%), Postives = 69/106 (65.09%), Query Frame = 1
Query:    1 MERCTAAFRVDARYKQDLRFLRIWLLYADICSNAVDVFNYLLHKEIGTEWELLYSCKAAVLENLGKYKEAEEALKLGVNREAKPLDKMENNHKRFLERMALRVAKA 318
            +ERCT  F    +Y+ D R+L++W++YAD+C   +++F YL   +IG+   L Y  KA + EN   Y++A+++ +LG+ R A+PLD+++  H+ F  RM + + KA
Sbjct:   86 LERCTNMFVQTEKYRNDQRYLKLWIIYADMCREPLEIFAYLETNKIGSTLSLTYEAKAIIYENKSNYQQADQSYQLGIQRGAQPLDRLKKKHEEFQRRMVMAMKKA 191          
BLAST of mRNA_M-pyrifera_M_contig99271.22839.1 vs. uniprot
Match: A0A151Z8B6_9MYCE (BUB1 N-terminal domain-containing protein n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151Z8B6_9MYCE)

HSP 1 Score: 92.4 bits (228), Expect = 6.970e-19
Identity = 43/103 (41.75%), Postives = 68/103 (66.02%), Query Frame = 1
Query:    1 MERCTAAFRVDARYKQDLRFLRIWLLYADICSNAVDVFNYLLHKEIGTEWELLYSCKAAVLENLGKYKEAEEALKLGVNREAKPLDKMENNHKRFLERMALRV 309
            +ERCT  F    +YK D R+LRIW+ YAD+CS+ V++F++L   +IG    LLY  +A V EN G Y++A+ A + G+ R+A+PL++++  H  F  R+  R+
Sbjct:   91 LERCTKLFLSWEKYKNDPRYLRIWITYADMCSDPVEIFSFLETNKIGCNLSLLYEARAIVYENKGNYQQADLAYQQGIQRKAQPLERLQQKHSDFERRLISRM 193          
BLAST of mRNA_M-pyrifera_M_contig99271.22839.1 vs. uniprot
Match: F4Q3S0_CAVFA (Uncharacterized protein bub1 n=1 Tax=Cavenderia fasciculata (strain SH3) TaxID=1054147 RepID=F4Q3S0_CAVFA)

HSP 1 Score: 92.0 bits (227), Expect = 9.590e-19
Identity = 51/152 (33.55%), Postives = 89/152 (58.55%), Query Frame = 1
Query:    1 MERCTAAFRVDARYKQDLRFLRIWLLYADICSNAVDVFNYLLHKEIGTEWELLYSCKAAVLENLGKYKEAEEALKLGVNREAKPLDKMENNHKRFLERMALRVAKAKQAKADRELVKGSADSSKSKPSSRTGSSRSSQSARPEPRRRALGTL 456
            ++RC   F    +YK D+R+LR+WL+YAD+C   +++FNYL    IG++  LLY  KA + EN   Y  A+++ +LG+ R A+PLD+++  H  F +R+   +        +++L+  S+ +S    S+   S+ ++    P  +R ALGT+
Sbjct:   87 LQRCNNLFVKSDQYKNDMRYLRLWLIYADMCREPIEIFNYLEVHGIGSQLSLLYEAKAIIYENKTNYPLADQSYQLGIQRRAQPLDRLQKKHTEFEKRLVHHMK-------NKDLLTASSSTSNQPASNVPPSTPNNNENTPTIQRAALGTI 231          
BLAST of mRNA_M-pyrifera_M_contig99271.22839.1 vs. uniprot
Match: F0ZUE3_DICPU (BUB1 N-terminal domain-containing protein n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0ZUE3_DICPU)

HSP 1 Score: 91.7 bits (226), Expect = 1.300e-18
Identity = 41/103 (39.81%), Postives = 69/103 (66.99%), Query Frame = 1
Query:    1 MERCTAAFRVDARYKQDLRFLRIWLLYADICSNAVDVFNYLLHKEIGTEWELLYSCKAAVLENLGKYKEAEEALKLGVNREAKPLDKMENNHKRFLERMALRV 309
            +ERCT  F    +YK D R+LRIW+ YAD+C + ++VF +L ++ IG    L+Y  +A V EN G Y++A++A K G+ R+A+P+++++  H+ F  R+  R+
Sbjct:   84 LERCTRLFINTEKYKNDPRYLRIWITYADMCRDPIEVFAFLENQRIGFYLSLIYEARAIVYENKGDYEKADKAFKQGIERKAQPIERLQQKHQDFERRLMARL 186          
BLAST of mRNA_M-pyrifera_M_contig99271.22839.1 vs. uniprot
Match: A0A5J4YN13_PORPP (Putative inactive serine/threonine-protein kinase bub1 n=2 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4YN13_PORPP)

HSP 1 Score: 91.3 bits (225), Expect = 1.750e-18
Identity = 48/111 (43.24%), Postives = 69/111 (62.16%), Query Frame = 1
Query:    1 MERCTAAFRVDARYKQDLRFLRIWLLYADICS-NAVDVFNYLLHKEIGTEWELLYSCKAAVLENLGKYKEAEEALKLGVNREAKPLDKMENNHKRFLERMALRVAKAKQAK 330
            +ERCT  F  DA+   D+R+LRIW+LYAD+ S +  D+F Y+   +IG +  L Y   A VLE   +Y  A++  + G+ R AKP +++EN HK F  RMALRV +  + K
Sbjct:  100 LERCTKYFAHDAQVANDIRYLRIWILYADLASPDPSDIFAYMYSNQIGLDLALFYEAYAIVLEKKRQYAMADKLYQEGIARSAKPPERLENRHKEFQHRMALRVERQNEQK 210          
BLAST of mRNA_M-pyrifera_M_contig99271.22839.1 vs. uniprot
Match: BUB1_DICDI (Probable inactive serine/threonine-protein kinase bub1 n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=BUB1_DICDI)

HSP 1 Score: 91.3 bits (225), Expect = 1.780e-18
Identity = 41/103 (39.81%), Postives = 68/103 (66.02%), Query Frame = 1
Query:    1 MERCTAAFRVDARYKQDLRFLRIWLLYADICSNAVDVFNYLLHKEIGTEWELLYSCKAAVLENLGKYKEAEEALKLGVNREAKPLDKMENNHKRFLERMALRV 309
            +ERCT  F    +YK D R+LRIW+ YAD+C + ++VF++L  + IG    LLY  +A V EN G Y++A+++ K G+ R+A+P+++++  H  F  R+  R+
Sbjct:   87 LERCTRLFLSTEKYKNDPRYLRIWITYADMCRDPIEVFSFLEVQRIGFYLSLLYEARAIVYENKGNYEQADKSFKQGIERKAQPIERLQQKHLDFERRLIARL 189          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig99271.22839.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI000C051E563.740e-2241.35mitotic checkpoint serine/threonine-protein kinase... [more]
A0A2B4RTS4_STYPI1.970e-2141.35Mitotic checkpoint serine/threonine-protein kinase... [more]
A0A8J4PY03_9MYCE3.640e-2144.66Uncharacterized protein n=1 Tax=Polysphondylium vi... [more]
D3AZV9_POLPP3.160e-2037.40Uncharacterized protein n=1 Tax=Polysphondylium pa... [more]
UPI000644EA2D3.760e-1937.74hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A151Z8B6_9MYCE6.970e-1941.75BUB1 N-terminal domain-containing protein n=1 Tax=... [more]
F4Q3S0_CAVFA9.590e-1933.55Uncharacterized protein bub1 n=1 Tax=Cavenderia fa... [more]
F0ZUE3_DICPU1.300e-1839.81BUB1 N-terminal domain-containing protein n=1 Tax=... [more]
A0A5J4YN13_PORPP1.750e-1843.24Putative inactive serine/threonine-protein kinase ... [more]
BUB1_DICDI1.780e-1839.81Probable inactive serine/threonine-protein kinase ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig99271contigM-pyrifera_M_contig99271:175..691 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score92.4
Seed ortholog evalue1.9e-16
Seed eggNOG ortholog44689.DDB0231500
KEGG koko:K02178,ko:K06637
KEGG Pathwayko04110,ko04111,ko04113,ko04114,ko04914,ko05166,map04110,map04111,map04113,map04114,map04914,map05166
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0000075,GO:0000228,GO:0000278,GO:0000280,GO:0000775,GO:0000776,GO:0000777,GO:0000778,GO:0000779,GO:0000780,GO:0000793,GO:0000794,GO:0000819,GO:0003674,GO:0003824,GO:0004672,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005694,GO:0005737,GO:0005819,GO:0005828,GO:0005856,GO:0005874,GO:0005876,GO:0006464,GO:0006468,GO:0006793,GO:0006796,GO:0006807,GO:0006996,GO:0007049,GO:0007059,GO:0007062,GO:0007088,GO:0007093,GO:0007094,GO:0007135,GO:0007346,GO:0008150,GO:0008152,GO:0009987,GO:0010369,GO:0010564,GO:0010639,GO:0010948,GO:0010965,GO:0015630,GO:0016043,GO:0016301,GO:0016310,GO:0016740,GO:0016772,GO:0016773,GO:0019538,GO:0022402,GO:0022414,GO:0030071,GO:0031577,GO:0031974,GO:0031981,GO:0032991,GO:0033043,GO:0033044,GO:0033045,GO:0033046,GO:0033047,GO:0033048,GO:0036211,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044430,GO:0044446,GO:0044454,GO:0044464,GO:0045132,GO:0045144,GO:0045786,GO:0045839,GO:0045841,GO:0045930,GO:0048285,GO:0048519,GO:0048523,GO:0050789,GO:0050794,GO:0051128,GO:0051129,GO:0051177,GO:0051276,GO:0051321,GO:0051726,GO:0051754,GO:0051783,GO:0051784,GO:0051983,GO:0051985,GO:0061983,GO:0065007,GO:0070013,GO:0070192,GO:0070601,GO:0071173,GO:0071174,GO:0071704,GO:0071840,GO:0098687,GO:0098813,GO:0099080,GO:0099081,GO:0099512,GO:0099513,GO:0140013,GO:0140096,GO:1901564,GO:1901987,GO:1901988,GO:1901990,GO:1901991,GO:1902099,GO:1902100,GO:1903046,GO:1903047,GO:1905818,GO:1905819,GO:2000816,GO:2001251
EggNOG free text desc.Mad3/BUB1 hoMad3/BUB1 homology region 1
EggNOG OGs3XFW9@554915,KOG1166@1,KOG1166@2759
EC2.7.11.1
COG Functional cat.D
Best tax levelAmoebozoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01001,ko03036
Exons2
Model size459
Cds size459
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816910.1578584-CDS-M-pyrifera_M_contig99271:174..5521622816910.1578584-CDS-M-pyrifera_M_contig99271:174..552Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99271 175..552 -
1692278250.2220883-CDS-M-pyrifera_M_contig99271:174..5521692278250.2220883-CDS-M-pyrifera_M_contig99271:174..552Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99271 175..552 -
1622816910.1742396-CDS-M-pyrifera_M_contig99271:610..6911622816910.1742396-CDS-M-pyrifera_M_contig99271:610..691Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99271 611..691 -
1692278250.2391274-CDS-M-pyrifera_M_contig99271:610..6911692278250.2391274-CDS-M-pyrifera_M_contig99271:610..691Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99271 611..691 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig99271.22839.1prot_M-pyrifera_M_contig99271.22839.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig99271 175..691 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig99271.22839.1

>prot_M-pyrifera_M_contig99271.22839.1 ID=prot_M-pyrifera_M_contig99271.22839.1|Name=mRNA_M-pyrifera_M_contig99271.22839.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=153bp
MERCTAAFRVDARYKQDLRFLRIWLLYADICSNAVDVFNYLLHKEIGTEW
ELLYSCKAAVLENLGKYKEAEEALKLGVNREAKPLDKMENNHKRFLERMA
LRVAKAKQAKADRELVKGSADSSKSKPSSRTGSSRSSQSARPEPRRRALG
TLA
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mRNA from alignment at M-pyrifera_M_contig99271:175..691-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig99271.22839.1 ID=mRNA_M-pyrifera_M_contig99271.22839.1|Name=mRNA_M-pyrifera_M_contig99271.22839.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=517bp|location=Sequence derived from alignment at M-pyrifera_M_contig99271:175..691- (Macrocystis pyrifera P11B4 male)
ATGGAGCGTTGCACAGCAGCTTTCAGAGTGGACGCTCGCTACAAGCAAGA CCTCCGCTTCCTTCGCATTTGGTTGCTCTACGTAAGTTTACCTCTCGCAC CTTACGCGATTCCTGCATAGCGCTGACCGCTCCGTTCAGGCTGATATTTG CAGCAATGCTGTGGACGTCTTCAACTATCTCCTTCACAAGGAGATTGGAA CGGAATGGGAGCTCCTCTACTCGTGCAAGGCGGCTGTACTGGAGAACCTC GGCAAGTACAAGGAGGCAGAAGAGGCCTTGAAGCTCGGCGTCAACAGAGA GGCCAAGCCCTTGGACAAGATGGAGAATAACCACAAGAGGTTCCTTGAGC GCATGGCTCTCCGCGTGGCCAAGGCCAAGCAGGCGAAGGCCGATCGAGAG CTGGTGAAGGGGTCTGCTGACTCCTCCAAGAGCAAGCCTTCCTCCAGGAC AGGATCCAGCAGGTCGTCGCAGTCAGCTCGACCTGAGCCACGGCGCCGAG CTCTGGGAACTCTTGCA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig99271:175..691-

>mRNA_M-pyrifera_M_contig99271.22839.1 ID=mRNA_M-pyrifera_M_contig99271.22839.1|Name=mRNA_M-pyrifera_M_contig99271.22839.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=918bp|location=Sequence derived from alignment at M-pyrifera_M_contig99271:175..691- (Macrocystis pyrifera P11B4 male)
ATGGAGCGTTGCACAGCAGCTTTCAGAGTGGACGCTCGCTACAAGCAAGA
CCTCCGCTTCCTTCGCATTTGGTTGCTCTACATGGAGCGTTGCACAGCAG
CTTTCAGAGTGGACGCTCGCTACAAGCAAGACCTCCGCTTCCTTCGCATT
TGGTTGCTCTACGCTGATATTTGCAGCAATGCTGTGGACGTCTTCAACTA
TCTCCTTCACAAGGAGATTGGAACGGAATGGGAGCTCCTCTACTCGTGCA
AGGCGGCTGTACTGGAGAACCTCGGCAAGTACAAGGAGGCAGAAGAGGCC
TTGAAGCTCGGCGTCAACAGAGAGGCCAAGCCCTTGGACAAGATGGAGAA
TAACCACAAGAGGTTCCTTGAGCGCATGGCTCTCCGCGTGGCCAAGGCCA
AGCAGGCGAAGGCCGATCGAGAGCTGGTGAAGGGGTCTGCTGACTCCTCC
AAGAGCAAGCCTTCCTCCAGGACAGGATCCAGCAGGTCGTCGCAGTCAGC
TCGACCTGAGCCACGGCGCCGAGCTCTGGGAACTCTTGCAGCTGATATTT
GCAGCAATGCTGTGGACGTCTTCAACTATCTCCTTCACAAGGAGATTGGA
ACGGAATGGGAGCTCCTCTACTCGTGCAAGGCGGCTGTACTGGAGAACCT
CGGCAAGTACAAGGAGGCAGAAGAGGCCTTGAAGCTCGGCGTCAACAGAG
AGGCCAAGCCCTTGGACAAGATGGAGAATAACCACAAGAGGTTCCTTGAG
CGCATGGCTCTCCGCGTGGCCAAGGCCAAGCAGGCGAAGGCCGATCGAGA
GCTGGTGAAGGGGTCTGCTGACTCCTCCAAGAGCAAGCCTTCCTCCAGGA
CAGGATCCAGCAGGTCGTCGCAGTCAGCTCGACCTGAGCCACGGCGCCGA
GCTCTGGGAACTCTTGCA
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