mRNA_M-pyrifera_M_contig9927.22838.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig9927.22838.1
Unique NamemRNA_M-pyrifera_M_contig9927.22838.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig9927.22838.1 vs. uniprot
Match: D8LHH2_ECTSI (CPLD51 protein required for cyt b6 assembly n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LHH2_ECTSI)

HSP 1 Score: 166 bits (421), Expect = 1.270e-48
Identity = 92/118 (77.97%), Postives = 99/118 (83.90%), Query Frame = 1
Query:    1 MVSPVELADAAVAVHHSASSALPSFLLGDEEI-----SVGYSKFSYYTTLGLYVLSFPGLWSVVKRATKTKWV-RLYEIPGPASTEAGAESVKQTAAKIIAYFQANNYKILEAGEEIT 336
            +V+P E+ DAAVA HH+ S ALPSFLL DE +     SVGYSKFSYYTTLGLYVLSFPGLWSVVKRATKTK+    YE+PGPASTE GAESVKQTAAKIIAYFQANNYKI EAGE IT
Sbjct:   76 VVAPHEVVDAAVAAHHAVS-ALPSFLLSDEAVAEAAGSVGYSKFSYYTTLGLYVLSFPGLWSVVKRATKTKYKDATYELPGPASTEKGAESVKQTAAKIIAYFQANNYKIKEAGETIT 192          
BLAST of mRNA_M-pyrifera_M_contig9927.22838.1 vs. uniprot
Match: A0A835YMB8_9STRA (CPLD51 protein required for cyt b6 assembly n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YMB8_9STRA)

HSP 1 Score: 107 bits (268), Expect = 2.630e-26
Identity = 55/81 (67.90%), Postives = 66/81 (81.48%), Query Frame = 1
Query:   97 SVGYSKFSYYTTLGLYVLSFPGLWSVVKRATKTKWV-RLYEIPGPASTEAGAESVKQTAAKIIAYFQANNYKILEAGEEIT 336
            SV YSK SYY TLGLY+LSFPGLWSV+KRA KTK+  R+Y + GPA+  A A+ VKQ AA+++AYFQANNYKI+ AGEEI 
Sbjct:   86 SVSYSKSSYYATLGLYLLSFPGLWSVIKRAAKTKYKERVYTVAGPAAAVA-AKPVKQVAAEVVAYFQANNYKIVSAGEEIV 165          
BLAST of mRNA_M-pyrifera_M_contig9927.22838.1 vs. uniprot
Match: A0A7S4HKN9_9EUKA (Hypothetical protein n=1 Tax=Prymnesium polylepis TaxID=72548 RepID=A0A7S4HKN9_9EUKA)

HSP 1 Score: 89.7 bits (221), Expect = 7.360e-20
Identity = 53/99 (53.54%), Postives = 69/99 (69.70%), Query Frame = 1
Query:   52 ASSALPSFLLGDEEISV---GYSKFSYYTTLGLYVLSFPGLWSVVKRATKTKWVR-LYEIPGPASTEAGAESVKQTAAKIIAYFQANNYKILEAGEEIT 336
            AS+ L +F   D+ +S+   GYS  S+Y TL LYVLSFPGL+S+V R+ K+K VR  YE+ GPAST  G   V++TA  I+AYFQANNYKI +A + I 
Sbjct:    4 ASAPLAAF--ADDAVSISENGYSSVSFYFTLVLYVLSFPGLYSLVTRSVKSKVVRKTYEVAGPAST--GGRPVRETAGDIVAYFQANNYKIADASDVIV 98          
BLAST of mRNA_M-pyrifera_M_contig9927.22838.1 vs. uniprot
Match: A0A7S2XLP2_9STRA (Hypothetical protein n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2XLP2_9STRA)

HSP 1 Score: 86.3 bits (212), Expect = 2.460e-18
Identity = 53/109 (48.62%), Postives = 71/109 (65.14%), Query Frame = 1
Query:   19 LADAAVAVHHSASSALPSFLLGDEEIS---VGYSKFSYYTTLGLYVLSFPGLWSVVKRATKTKWVRLYEIPGPASTEAGAESVKQTAAKIIAYFQANNYKILEAGEEIT 336
            L+DAA  +   +SSA  +  +GD   S   V YSK SYYTTLGLY LSFPGLWS +KR+T  K  R   +    + E G + ++Q A +I+AY +ANNY+++EAGE IT
Sbjct:   18 LSDAAADLADPSSSAPVN--VGDAGFSTDPVTYSKASYYTTLGLYALSFPGLWSQIKRSTAAKTKRKTFVSSGEAVE-GGKDLRQQAGEIMAYMKANNYEVVEAGETIT 123          
BLAST of mRNA_M-pyrifera_M_contig9927.22838.1 vs. uniprot
Match: A0A7S2BPD7_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Haptolina brevifila TaxID=156173 RepID=A0A7S2BPD7_9EUKA)

HSP 1 Score: 83.2 bits (204), Expect = 4.290e-18
Identity = 43/78 (55.13%), Postives = 58/78 (74.36%), Query Frame = 1
Query:  103 GYSKFSYYTTLGLYVLSFPGLWSVVKRATKTKWVR-LYEIPGPASTEAGAESVKQTAAKIIAYFQANNYKILEAGEEI 333
            G+S+ SYYTTL LYV+SFPG++S+VKR+ K+K VR  YE+ GPAS   G    ++ A  I+A+FQANNYKI +A + I
Sbjct:   62 GFSQASYYTTLALYVVSFPGVYSLVKRSVKSKIVRKTYEVAGPASD--GGRPTRELAGDIVAFFQANNYKITDATDTI 137          
BLAST of mRNA_M-pyrifera_M_contig9927.22838.1 vs. uniprot
Match: A0A7S3XQU3_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XQU3_HETAK)

HSP 1 Score: 87.0 bits (214), Expect = 4.370e-18
Identity = 49/103 (47.57%), Postives = 66/103 (64.08%), Query Frame = 1
Query:   88 EEISVGYSKFSYYTTLGLYVLSFPGLWSVVKRATKTKWVR-LYEIPGPASTEAGAESVKQTAAK-------------------IIAYFQANNYKILEAGEEIT 336
            EE +  YSK SYYTTLGLYV+SFPGL+S+VKR+ K   V+  YE+PGP S + GA+ +KQ AA+                   ++AYF+A NY+I+ AGE I+
Sbjct:   75 EEATTSYSKASYYTTLGLYVISFPGLYSLVKRSVKASNVKKTYELPGP-SADTGAKEMKQIAAEAVREAYLPIIEAAAKQLKQVVAYFRAQNYEIVNAGETIS 176          
BLAST of mRNA_M-pyrifera_M_contig9927.22838.1 vs. uniprot
Match: A0A7S3EZW5_9EUKA (Hypothetical protein n=1 Tax=Haptolina ericina TaxID=156174 RepID=A0A7S3EZW5_9EUKA)

HSP 1 Score: 85.9 bits (211), Expect = 4.840e-18
Identity = 44/79 (55.70%), Postives = 59/79 (74.68%), Query Frame = 1
Query:  103 GYSKFSYYTTLGLYVLSFPGLWSVVKRATKTKWVR-LYEIPGPASTEAGAESVKQTAAKIIAYFQANNYKILEAGEEIT 336
            G+S+ SYYTTL LYVLSFPG++S+VKR+ K+K VR  YE+ GPA+ E  A   ++ A  I+A+FQANNYKI +A + I 
Sbjct:   68 GFSQVSYYTTLALYVLSFPGIYSLVKRSVKSKVVRKTYEVAGPAAAEGRA--TREVAGDIVAFFQANNYKIADASDVIV 144          
BLAST of mRNA_M-pyrifera_M_contig9927.22838.1 vs. uniprot
Match: A0A7R9ZEM2_9STRA (Hypothetical protein n=1 Tax=Pseudictyota dubia TaxID=2749911 RepID=A0A7R9ZEM2_9STRA)

HSP 1 Score: 84.0 bits (206), Expect = 1.280e-17
Identity = 41/77 (53.25%), Postives = 57/77 (74.03%), Query Frame = 1
Query:  106 YSKFSYYTTLGLYVLSFPGLWSVVKRATKTKWVRLYEIPGPASTEAGAESVKQTAAKIIAYFQANNYKILEAGEEIT 336
            YSK+S+YTTLG+YV+SFPGLWS +KR+TK K  R   +    + E G + ++Q A +I+AY +ANNY++ EAGE IT
Sbjct:   26 YSKWSFYTTLGVYVMSFPGLWSQIKRSTKAKVKRKTYVSDGENAE-GGKDMRQQAGEIMAYMKANNYEVEEAGETIT 101          
BLAST of mRNA_M-pyrifera_M_contig9927.22838.1 vs. uniprot
Match: A0A1Z5K036_FISSO (Uncharacterized protein n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K036_FISSO)

HSP 1 Score: 84.0 bits (206), Expect = 2.650e-17
Identity = 48/95 (50.53%), Postives = 64/95 (67.37%), Query Frame = 1
Query:   55 SSALPSFLLGDEEIS-VGYSKFSYYTTLGLYVLSFPGLWSVVKRATKTKWVRLYEIPGPASTEAGAESVKQTAAKIIAYFQANNYKILEAGEEIT 336
            SS L S + G  E+  V YSK SYYT LGLYVLSFPG+WS VKR+T  K  R   +    +  AG +S++Q A +I+AY  +NNY++++AGE IT
Sbjct:   52 SSLLLSAVEGPPEVGGVSYSKASYYTILGLYVLSFPGVWSQVKRSTSAKIKRKTFVTAGENAAAG-KSLRQQAGEIMAYMTSNNYEVVDAGETIT 145          
BLAST of mRNA_M-pyrifera_M_contig9927.22838.1 vs. uniprot
Match: A0A7R9XVV1_9VIRI (Hypothetical protein n=1 Tax=Prasinoderma coloniale TaxID=156133 RepID=A0A7R9XVV1_9VIRI)

HSP 1 Score: 82.8 bits (203), Expect = 4.670e-17
Identity = 43/80 (53.75%), Postives = 57/80 (71.25%), Query Frame = 1
Query:  103 GYSKFSYYTTLGLYVLSFPGLWSVVKRATKTKWV-RLYEIPGPASTEA-GAESVKQTAAKIIAYFQANNYKILEAGEEIT 336
            GY+K SYYTTLGLY+ S PGL+S+VKR+TK K V + Y +PGPA TEA G + +   A ++  +F  NNY I +AGE +T
Sbjct:   42 GYAKSSYYTTLGLYLFSLPGLYSLVKRSTKAKVVQKTYAVPGPAVTEAEGGKPLDALAREVSTFFIRNNYNITDAGEIVT 121          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig9927.22838.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LHH2_ECTSI1.270e-4877.97CPLD51 protein required for cyt b6 assembly n=2 Ta... [more]
A0A835YMB8_9STRA2.630e-2667.90CPLD51 protein required for cyt b6 assembly n=1 Ta... [more]
A0A7S4HKN9_9EUKA7.360e-2053.54Hypothetical protein n=1 Tax=Prymnesium polylepis ... [more]
A0A7S2XLP2_9STRA2.460e-1848.62Hypothetical protein n=1 Tax=Attheya septentrional... [more]
A0A7S2BPD7_9EUKA4.290e-1855.13Hypothetical protein (Fragment) n=1 Tax=Haptolina ... [more]
A0A7S3XQU3_HETAK4.370e-1847.57Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
A0A7S3EZW5_9EUKA4.840e-1855.70Hypothetical protein n=1 Tax=Haptolina ericina Tax... [more]
A0A7R9ZEM2_9STRA1.280e-1753.25Hypothetical protein n=1 Tax=Pseudictyota dubia Ta... [more]
A0A1Z5K036_FISSO2.650e-1750.53Uncharacterized protein n=2 Tax=Fistulifera solari... [more]
A0A7R9XVV1_9VIRI4.670e-1753.75Hypothetical protein n=1 Tax=Prasinoderma colonial... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig9927contigM-pyrifera_M_contig9927:9368..10544 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score167.9
Seed ortholog evalue2.7e-39
Seed eggNOG ortholog2880.D8LHH2
Preferred nameCCB1
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0008150,GO:0009507,GO:0009536,GO:0009987,GO:0010190,GO:0016043,GO:0017004,GO:0022607,GO:0034622,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043933,GO:0044085,GO:0044424,GO:0044444,GO:0044464,GO:0065003,GO:0071840
EggNOG free text desc.Cofactor assembly of complex C subunit B
EggNOG OGs2AZTV@1,2QR1Q@2759
Ec32 ortholog descriptionCPLD51 protein required for cyt b6 assembly
Ec32 orthologEc-11_004090.1
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons2
Model size339
Cds size339
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816910.1070335-CDS-M-pyrifera_M_contig9927:9367..95751622816910.1070335-CDS-M-pyrifera_M_contig9927:9367..9575Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9927 9368..9575 +
1692278250.2027593-CDS-M-pyrifera_M_contig9927:9367..95751692278250.2027593-CDS-M-pyrifera_M_contig9927:9367..9575Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9927 9368..9575 +
1622816910.1211221-CDS-M-pyrifera_M_contig9927:10413..105441622816910.1211221-CDS-M-pyrifera_M_contig9927:10413..10544Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9927 10414..10544 +
1692278250.2129443-CDS-M-pyrifera_M_contig9927:10413..105441692278250.2129443-CDS-M-pyrifera_M_contig9927:10413..10544Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9927 10414..10544 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig9927.22838.1prot_M-pyrifera_M_contig9927.22838.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig9927 9368..10544 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig9927.22838.1

>prot_M-pyrifera_M_contig9927.22838.1 ID=prot_M-pyrifera_M_contig9927.22838.1|Name=mRNA_M-pyrifera_M_contig9927.22838.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=113bp
MVSPVELADAAVAVHHSASSALPSFLLGDEEISVGYSKFSYYTTLGLYVL
SFPGLWSVVKRATKTKWVRLYEIPGPASTEAGAESVKQTAAKIIAYFQAN
NYKILEAGEEIT*
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mRNA from alignment at M-pyrifera_M_contig9927:9368..10544+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig9927.22838.1 ID=mRNA_M-pyrifera_M_contig9927.22838.1|Name=mRNA_M-pyrifera_M_contig9927.22838.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=1177bp|location=Sequence derived from alignment at M-pyrifera_M_contig9927:9368..10544+ (Macrocystis pyrifera P11B4 male)
ATGGTCTCCCCGGTCGAACTGGCGGACGCGGCGGTCGCCGTGCATCACTC AGCCTCGTCAGCGCTGCCGTCGTTTCTCCTCGGCGATGAGGAGATAAGCG TGGGCTACTCGAAGTTCAGCTACTACACCACGCTCGGGCTGTACGTCCTG TCGTTCCCTGGGCTGTGGTCCGTCGTCAAGCGAGCGACGAAGACCAAGTG GGTCCGACGTTAAGATGACCCCTTTACTACTGTGAATAGTATATTACACT ACTTCCAAGTAGAATTTACCCCAAAAACCTGGTTGCAGTTTCAAGGGGGG TAAGGTCGGGGGAGGGCAGAGGGGGGCAGCGGGGAGAGGCAAGGAAAGAG AAAACGCTCTCAGACAACTTTCCGATCCTTGTGCCCCTGAAAAACGCAAA TGTTGCCACTGGAAGGACGCTGCGCAAACATGCGGCTAGCGGAGTCAGCG CTCTGTTTCAGAGCTGCCGAGGGAGAGAGTCAAGCGTCCGTGTGTTTTTT GGTGAGGGGCTCGGAGCGCTGGGCCCTTGGGGTTTGCGTACGTACCGATA CATGCTTGATTGAAGGCGGGGGGGGGGGTGCTGTCTGGCCCGCACAACAG GAACGGCCTCGTATATACGTGGACGGTAGTGATTGTTCACACTTTGCGAA ACGCCCTTTATTTTATTGACCAAACTCAAAAAACTATAGCCGACACACTT CAGTACGACGGGAGTGGCGAGGATCGTCGGTGACTCCACTGACGAACGCA CTGTAACTCACGAAAAAATCGGTTCAATTTGGGTTTTTATCGGCCGCTTC GTTATTTCACCTCCCCCCGCCCCCCCACCTCCCGAGCGACCGTCTTTTTG ACTCTCTTGCTCGCCGAGCCGTTTGGAAAGCGCCTCGGGTGCATCTCCAG TAGACGGGGCCTCACTCGCCCCCCCCCCCCTACTTCACCCCCCCCCCCCG ACCTCCCCACCCCACTCCTCATCATCCCATTCCACCCCACCCCCCTCGAA CACCCCGCGCTTTCCCGTATTTCGCTGTGTAGGTACAAGGAGGCAGTCTA CGAGATTCCCGGACCGGCGTCGACGGAGGCGGGAGCGGAATCGGTGAAGC AGACGGCCGCGAAGATTATCGCGTACTTCCAAGCGAACAACTACAAGATC CTCGAGGCGGGGGAGGAGATCACGTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig9927:9368..10544+

>mRNA_M-pyrifera_M_contig9927.22838.1 ID=mRNA_M-pyrifera_M_contig9927.22838.1|Name=mRNA_M-pyrifera_M_contig9927.22838.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=678bp|location=Sequence derived from alignment at M-pyrifera_M_contig9927:9368..10544+ (Macrocystis pyrifera P11B4 male)
ATGGTCTCCCCGGTCGAACTGGCGGACGCGGCGGTCGCCGTGCATCACTC
AGCCTCGTCAGCGCTGCCGTCGTTTCTCCTCGGCGATGAGGAGATAAGCG
TGGGCTACTCGAAGTTCAGCTACTACACCACGCTCGGGCTGTACGTCCTG
TCGTTCCCTGGGCTGTGGTCCGTCGTCAAGCGAGCGACGAAGACCAAGTG
GGTCCGACATGGTCTCCCCGGTCGAACTGGCGGACGCGGCGGTCGCCGTG
CATCACTCAGCCTCGTCAGCGCTGCCGTCGTTTCTCCTCGGCGATGAGGA
GATAAGCGTGGGCTACTCGAAGTTCAGCTACTACACCACGCTCGGGCTGT
ACGTCCTGTCGTTCCCTGGGCTGTGGTCCGTCGTCAAGCGAGCGACGAAG
ACCAAGTGGGTCCGACTCTACGAGATTCCCGGACCGGCGTCGACGGAGGC
GGGAGCGGAATCGGTGAAGCAGACGGCCGCGAAGATTATCGCGTACTTCC
AAGCGAACAACTACAAGATCCTCGAGGCGGGGGAGGAGATCACGTGATCT
ACGAGATTCCCGGACCGGCGTCGACGGAGGCGGGAGCGGAATCGGTGAAG
CAGACGGCCGCGAAGATTATCGCGTACTTCCAAGCGAACAACTACAAGAT
CCTCGAGGCGGGGGAGGAGATCACGTGA
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