mRNA_M-pyrifera_M_contig98697.22718.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig98697.22718.1
Unique NamemRNA_M-pyrifera_M_contig98697.22718.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig98697.22718.1 vs. uniprot
Match: UPI00109BF2D8 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 n=1 Tax=Exaiptasia diaphana TaxID=2652724 RepID=UPI00109BF2D8)

HSP 1 Score: 119 bits (299), Expect = 3.230e-28
Identity = 70/172 (40.70%), Postives = 104/172 (60.47%), Query Frame = 1
Query:    1 YVLKPSWMREPITLSSSSYDPSGASVQPSIRLMVSVLAGKYLPKPAGALE-GEVIDPYVEVRLCGCPADNATNCTPVVENDGFNPRWADGSGKNVFTYDIRRPETALLLFVVRDSDMGPDELVGWYGLRLPMVRQGLRLVPLLDQHSVPLQVSTLFVHVSID-----EIDKD 498
            YVLKP +MR+     S SYDP     Q    L + +++G+ LPKPAG  E GEVIDPYVE+++ G  +D AT  + VV+++GFNP+W     K     D+  P+ A+L F V D D+G D+ +G + L +  ++ G R V LLD    P+  +T+FVHV+++     +IDK+
Sbjct:  647 YVLKPEYMRD----ESISYDPKHPPTQRKT-LKIHLISGQQLPKPAGHSEKGEVIDPYVEIKIYGVSSDQATFKSKVVKDNGFNPKWNLHVEK-----DLHVPDIAMLRFAVYDEDVGVDDFIGQFCLPITNLQTGYRHVHLLDMKGAPIPNATIFVHVTLENTSQSKIDKE 808          
BLAST of mRNA_M-pyrifera_M_contig98697.22718.1 vs. uniprot
Match: UPI00138FA2EE (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 isoform X1 n=3 Tax=Nematostella vectensis TaxID=45351 RepID=UPI00138FA2EE)

HSP 1 Score: 117 bits (292), Expect = 2.830e-27
Identity = 64/166 (38.55%), Postives = 102/166 (61.45%), Query Frame = 1
Query:    1 YVLKPSWMREPITLSSSSYDPSGASVQPSIR---LMVSVLAGKYLPKPAGAL-EGEVIDPYVEVRLCGCPADNATNCTPVVENDGFNPRWADGSGKNVFTYDIRRPETALLLFVVRDSDMGPDELVGWYGLRLPMVRQGLRLVPLLDQHSVPLQVSTLFVHVSIDE 486
            YVLKP++MR P       ++P+     P ++   L++ V++G+ LPKP G+  +GEVIDPYVEV++ G  +D+A   T V++++GFNP W +   K ++      PE A+L F V D D+G D+ +G + L +  +R G R + LLD    PL  ++LFVHV +++
Sbjct:  667 YVLKPAYMRNP----DIKFNPNNPKSCPGVKKKLLIIQVISGQQLPKPQGSSSKGEVIDPYVEVKIYGVNSDSADFKTKVIKDNGFNPVWNEEIKKLLYV-----PELAMLRFCVYDEDVGKDDFIGQFSLPITSLRTGYRHIHLLDIKGEPLPHASLFVHVMLED 823          
BLAST of mRNA_M-pyrifera_M_contig98697.22718.1 vs. uniprot
Match: A0A293MF90_ORNER (Phosphoinositide phospholipase C (Fragment) n=1 Tax=Ornithodoros erraticus TaxID=265619 RepID=A0A293MF90_ORNER)

HSP 1 Score: 111 bits (277), Expect = 3.430e-27
Identity = 62/165 (37.58%), Postives = 99/165 (60.00%), Query Frame = 1
Query:    1 YVLKPSWMREPITLSSSSYDPSGASVQPSIR--LMVSVLAGKYLPKPAGALEGEVIDPYVEVRLCGCPADNATNCTPVVENDGFNPRWADGSGKNVFTYDIRRPETALLLFVVRDSD-MGPDELVGWYGLRLPMVRQGLRLVPLLDQHSVPLQVSTLFVHVSIDE 486
            YVL P ++R+       +++PS   V PS++  L + +++G+ +PKP  A EGEV+DPYV +++ G P DN    T  + N+GFNP W +     +F  DIR PE A++LF V+D + +G +  +G Y L    + +G R V L ++   PL  +++FVHV+I E
Sbjct:   84 YVLMPEFLRK------GTFNPS--KVDPSLKRTLTIKIISGQQIPKPMAAAEGEVVDPYVIIKVKGHPLDNQKVKTKYIHNNGFNPYWGE-----IFELDIRVPELAMILFTVKDENKIGKNMKLGKYALPFNAIAEGYRHVHLRNEAFCPLVPASIFVHVTIRE 235          
BLAST of mRNA_M-pyrifera_M_contig98697.22718.1 vs. uniprot
Match: A7SDH9_NEMVE (Phosphoinositide phospholipase C (Fragment) n=1 Tax=Nematostella vectensis TaxID=45351 RepID=A7SDH9_NEMVE)

HSP 1 Score: 115 bits (288), Expect = 8.860e-27
Identity = 64/164 (39.02%), Postives = 100/164 (60.98%), Query Frame = 1
Query:    1 YVLKPSWMREPITLSSSSYDPSGASVQPSIR---LMVSVLAGKYLPKPAGAL-EGEVIDPYVEVRLCGCPADNATNCTPVVENDGFNPRWADGSGKNVFTYDIRRPETALLLFVVRDSDMGPDELVGWYGLRLPMVRQGLRLVPLLDQHSVPLQVSTLFVHVSI 480
            YVLKP++MR P       ++P+     P ++   L++ V++G+ LPKP G+  +GEVIDPYVEV++ G  +D+A   T V++++GFNP W +   K ++      PE A+L F V D D+G D+ +G + L +  +R G R + LLD    PL  ++LFVHV +
Sbjct:  511 YVLKPAYMRNP----DIKFNPNNPKSCPGVKKKLLIIQVISGQQLPKPQGSSSKGEVIDPYVEVKIYGVNSDSADFKTKVIKDNGFNPVWNEEIKKLLYV-----PELAMLRFCVYDEDVGKDDFIGQFSLPITSLRTGYRHIHLLDIKGEPLPHASLFVHVML 665          
BLAST of mRNA_M-pyrifera_M_contig98697.22718.1 vs. uniprot
Match: A0A5A8CDK2_CAFRO (Phosphoinositide phospholipase C n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CDK2_CAFRO)

HSP 1 Score: 114 bits (286), Expect = 1.790e-26
Identity = 63/166 (37.95%), Postives = 97/166 (58.43%), Query Frame = 1
Query:    1 YVLKPSWMREPITLSSSSYDPSGASVQP----SIRLMVSVLAGKYLPKPAGALEG-EVIDPYVEVRLCGCPADNATNCTPVVENDGFNPRWADG---SGKNVFTYDIRRPETALLLFVVRDSDMGPDELVGWYGLRLPMVRQGLRLVPLLDQHSVPLQVSTLFVHV 474
            YVLKP+WMR    ++   +  S +  +P    S++L V+V++  +LPKP    +G ++IDP+VEVR+ G PAD A + T V+ ++GFNP W D    S K   T+ + RP+ A+L F V D ++    +V   G+ L  +R G R + LLD  S+PL  + L V +
Sbjct:  625 YVLKPAWMRSDGPIAPFHHSGSPSQRRPTGTRSVKLTVTVISAHFLPKPREYKDGRDIIDPFVEVRVAGAPADEACHTTGVISDNGFNPCWVDPAKPSLKPTATFTVGRPDVAVLTFSVSDKNLAGSVIVAQAGVPLAALRTGTRAIALLDSLSLPLPATKLIVRI 790          
BLAST of mRNA_M-pyrifera_M_contig98697.22718.1 vs. uniprot
Match: A0A2J7R1L2_9NEOP (Phosphoinositide phospholipase C n=4 Tax=Cryptotermes secundus TaxID=105785 RepID=A0A2J7R1L2_9NEOP)

HSP 1 Score: 111 bits (277), Expect = 2.320e-26
Identity = 62/163 (38.04%), Postives = 95/163 (58.28%), Query Frame = 1
Query:    1 YVLKPSWMREPITLSSSSYDPSGASV-QPSIRLMVSVLAGKYLPKPAGALEGEVIDPYVEVRLCGCPADNATNCTPVVENDGFNPRWADGSGKNVFTYDIRRPETALLLFVVRD-SDMGPDELVGWYGLRLPMVRQGLRLVPLLDQHSVPLQVSTLFVHVSID 483
            YVLKP ++       +  YDP      + S +L + +++G+++PK    L+GEV+DPYV V++ G PAD     T +V N+GFNPRW         ++ ++ PE A++ F+VRD S  G D ++G Y L    +++G R V LLD     +  +TLFVHVSI+
Sbjct:  178 YVLKPKFL-----CGTGDYDPDAVMAHRASTQLKLIIISGQHIPKANQDLQGEVVDPYVVVKIVGHPADAQKKKTDIVRNNGFNPRW-----NQEMSFSLKVPELAMVHFIVRDCSTTGKDAMLGMYALPFTSIQEGYRHVYLLDYLRNLVSPATLFVHVSIE 330          
BLAST of mRNA_M-pyrifera_M_contig98697.22718.1 vs. uniprot
Match: A0A6P8JC04_ACTTE (Phosphoinositide phospholipase C n=2 Tax=Actinia tenebrosa TaxID=6105 RepID=A0A6P8JC04_ACTTE)

HSP 1 Score: 113 bits (283), Expect = 4.570e-26
Identity = 63/168 (37.50%), Postives = 97/168 (57.74%), Query Frame = 1
Query:    1 YVLKPSWMREPITLSSSSYDPSGASVQPSIRLMVSVLAGKYLPKPAG-ALEGEVIDPYVEVRLCGCPADNATNCTPVVENDGFNPRWADGSGKNVFTYDIRRPETALLLFVVRDSDMGPDELVGWYGLRLPMVRQGLRLVPLLDQHSVPLQVSTLFVHVSIDEIDKDG 501
            YVLKP +MR+P    +  + P G        L + V++G+ LPKP G + +GEVIDPYVEV++ G P+D  T  + VV+++GFNP W     K     ++  PE  ++ F V D D+G D+ +G + L    ++ G R V LL+    P+  +T+FVHV+I++  K  
Sbjct:  653 YVLKPEFMRDPSIDFNPKHPPEGLRKL----LKIEVISGQQLPKPEGESSKGEVIDPYVEVKIYGLPSDQGTFKSKVVKDNGFNPIWNLEVEK-----ELHVPELVMIRFAVLDEDVGKDDFIGQFSLPFTSLQTGYRHVHLLNMKGEPIPHATIFVHVTIEDNPKSS 811          
BLAST of mRNA_M-pyrifera_M_contig98697.22718.1 vs. uniprot
Match: A0A067R648_ZOONE (Phosphoinositide phospholipase C n=4 Tax=Zootermopsis nevadensis TaxID=136037 RepID=A0A067R648_ZOONE)

HSP 1 Score: 112 bits (280), Expect = 1.140e-25
Identity = 61/162 (37.65%), Postives = 95/162 (58.64%), Query Frame = 1
Query:    1 YVLKPSWMREPITLSSSSYDPSGA-SVQPSIRLMVSVLAGKYLPKPAGALEGEVIDPYVEVRLCGCPADNATNCTPVVENDGFNPRWADGSGKNVFTYDIRRPETALLLFVVRDSDM-GPDELVGWYGLRLPMVRQGLRLVPLLDQHSVPLQVSTLFVHVSI 480
            YVLKP ++       +  YDP      +PS +L +++++G+++PK    LEGEV+DPYV V++ G PAD     T ++ N+GFNPRW +        + ++ PE A++ F+V+DS   G D  +G Y L    ++QG R + L+D     +  +TLFVHVSI
Sbjct:  628 YVLKPKFL-----CGNGDYDPDAVIKHRPSAQLKLTIISGQHIPKANQDLEGEVVDPYVTVKIIGHPADEQKLKTDIIRNNGFNPRWNEE-----MNFSLKVPELAMVHFIVKDSSTTGKDATLGMYALPFSSMQQGYRHIYLVDYMGSLVSPATLFVHVSI 779          
BLAST of mRNA_M-pyrifera_M_contig98697.22718.1 vs. uniprot
Match: A0A1Z5LGQ3_ORNMO (Phosphoinositide phospholipase C (Fragment) n=2 Tax=Ornithodoros moubata TaxID=6938 RepID=A0A1Z5LGQ3_ORNMO)

HSP 1 Score: 106 bits (265), Expect = 5.360e-25
Identity = 57/163 (34.97%), Postives = 93/163 (57.06%), Query Frame = 1
Query:    1 YVLKPSWMREPITLSSSSYDPSGASVQPSIRLMVSVLAGKYLPKPAGALEGEVIDPYVEVRLCGCPADNATNCTPVVENDGFNPRWADGSGKNVFTYDIRRPETALLLFVVRDSDM-GPDELVGWYGLRLPMVRQGLRLVPLLDQHSVPLQVSTLFVHVSIDE 486
            YVL P ++R      +++++P+         L + +++G+ +PKP  A EGEV+DPYV +++ G P DN    T  + N+GFNP W +     +F  DIR PE A++LF V+D +  G +  +G Y L    + +G R + L +    PL  +++FVHV+I E
Sbjct:  129 YVLMPEFLR------NATFNPNKLDPGMKRTLTIKIISGQQIPKPMAAAEGEVVDPYVIIKVKGHPLDNQKVKTKFIHNNGFNPHWEE-----IFELDIRVPELAMILFTVKDENRTGKNMKLGKYALPFTAIAEGYRHIHLRNDAFSPLVPASIFVHVTIRE 280          
BLAST of mRNA_M-pyrifera_M_contig98697.22718.1 vs. uniprot
Match: UPI000BA82A63 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2-like isoform X1 n=3 Tax=Limulus polyphemus TaxID=6850 RepID=UPI000BA82A63)

HSP 1 Score: 108 bits (270), Expect = 1.650e-24
Identity = 58/163 (35.58%), Postives = 97/163 (59.51%), Query Frame = 1
Query:    1 YVLKPSWMREPITLSSSSYDPSG-ASVQPSIRLMVSVLAGKYLPKPAGALEGEVIDPYVEVRLCGCPADNATNCTPVVENDGFNPRWADGSGKNVFTYDIRRPETALLLFVVRDSDM-GPDELVGWYGLRLPMVRQGLRLVPLLDQHSVPLQVSTLFVHVSID 483
            YVLKP ++R+       S+DPS     +    L V V++G+++PKP  A  GE++DPYV +++CG P D+    T VV+N+GF P W +       T+D++ P+ A+++F V+D  + G +  +G Y L    + +G R + L+D +   +  +TLFVHV+I+
Sbjct:  365 YVLKPHFLRD------GSFDPSNLVENRYKKTLTVMVISGQHIPKPGEATVGEIVDPYVSIKVCGHPVDSNKKKTSVVKNNGFFPVWNE-----TLTFDVQMPDLAVVVFTVKDESITGRNFRLGRYALPFNSITEGYRHIHLVDDYHSVIVSATLFVHVTIE 516          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig98697.22718.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI00109BF2D83.230e-2840.701-phosphatidylinositol 4,5-bisphosphate phosphodie... [more]
UPI00138FA2EE2.830e-2738.551-phosphatidylinositol 4,5-bisphosphate phosphodie... [more]
A0A293MF90_ORNER3.430e-2737.58Phosphoinositide phospholipase C (Fragment) n=1 Ta... [more]
A7SDH9_NEMVE8.860e-2739.02Phosphoinositide phospholipase C (Fragment) n=1 Ta... [more]
A0A5A8CDK2_CAFRO1.790e-2637.95Phosphoinositide phospholipase C n=3 Tax=Cafeteria... [more]
A0A2J7R1L2_9NEOP2.320e-2638.04Phosphoinositide phospholipase C n=4 Tax=Cryptoter... [more]
A0A6P8JC04_ACTTE4.570e-2637.50Phosphoinositide phospholipase C n=2 Tax=Actinia t... [more]
A0A067R648_ZOONE1.140e-2537.65Phosphoinositide phospholipase C n=4 Tax=Zootermop... [more]
A0A1Z5LGQ3_ORNMO5.360e-2534.97Phosphoinositide phospholipase C (Fragment) n=2 Ta... [more]
UPI000BA82A631.650e-2435.581-phosphatidylinositol 4,5-bisphosphate phosphodie... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig98697contigM-pyrifera_M_contig98697:194..697 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeOpisthokonta
Seed ortholog score111.7
Seed ortholog evalue3.2e-22
Seed eggNOG ortholog192875.XP_004365118.1
Hectar predicted targeting categoryother localisation
EggNOG free text desc.C2 domain
EggNOG OGs38BAR@33154,COG5038@1,KOG0169@1,KOG0169@2759,KOG1012@2759
COG Functional cat.M
Best tax levelOpisthokonta
Best eggNOG OGNA|NA|NA
Exons1
Model size504
Cds size483
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816904.0933492-CDS-M-pyrifera_M_contig98697:193..6761622816904.0933492-CDS-M-pyrifera_M_contig98697:193..676Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig98697 194..676 -
1692278246.6902397-CDS-M-pyrifera_M_contig98697:193..6761692278246.6902397-CDS-M-pyrifera_M_contig98697:193..676Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig98697 194..676 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816904.1085296-UTR-M-pyrifera_M_contig98697:676..6971622816904.1085296-UTR-M-pyrifera_M_contig98697:676..697Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig98697 677..697 -
1692278246.714137-UTR-M-pyrifera_M_contig98697:676..6971692278246.714137-UTR-M-pyrifera_M_contig98697:676..697Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig98697 677..697 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig98697.22718.1prot_M-pyrifera_M_contig98697.22718.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig98697 194..676 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig98697.22718.1

>prot_M-pyrifera_M_contig98697.22718.1 ID=prot_M-pyrifera_M_contig98697.22718.1|Name=mRNA_M-pyrifera_M_contig98697.22718.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=161bp
MREPITLSSSSYDPSGASVQPSIRLMVSVLAGKYLPKPAGALEGEVIDPY
VEVRLCGCPADNATNCTPVVENDGFNPRWADGSGKNVFTYDIRRPETALL
LFVVRDSDMGPDELVGWYGLRLPMVRQGLRLVPLLDQHSVPLQVSTLFVH
VSIDEIDKDGN
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mRNA from alignment at M-pyrifera_M_contig98697:194..697-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig98697.22718.1 ID=mRNA_M-pyrifera_M_contig98697.22718.1|Name=mRNA_M-pyrifera_M_contig98697.22718.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=504bp|location=Sequence derived from alignment at M-pyrifera_M_contig98697:194..697- (Macrocystis pyrifera P11B4 male)
TATGTGTTGAAACCAAGTTGGATGCGTGAGCCAATCACACTGTCCTCCTC ATCCTATGATCCTTCTGGTGCTTCAGTTCAACCATCTATCCGACTCATGG TGTCTGTATTGGCTGGAAAGTACTTGCCAAAACCTGCAGGAGCATTGGAA GGTGAAGTGATTGATCCTTACGTTGAAGTACGCTTGTGTGGTTGTCCTGC GGACAATGCAACGAATTGCACTCCAGTCGTGGAGAATGACGGATTCAACC CAAGATGGGCTGATGGAAGTGGAAAGAATGTGTTTACATACGACATTCGG CGCCCAGAAACTGCTCTGCTACTCTTTGTAGTTCGAGATTCTGACATGGG ACCTGATGAGTTGGTTGGTTGGTATGGTCTACGTCTTCCAATGGTACGCC AAGGATTACGCCTTGTTCCTCTCCTTGATCAACATTCCGTCCCACTACAA GTGTCTACACTCTTTGTACATGTTTCTATTGATGAAATCGACAAGGATGG CAAC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig98697:194..697-

>mRNA_M-pyrifera_M_contig98697.22718.1 ID=mRNA_M-pyrifera_M_contig98697.22718.1|Name=mRNA_M-pyrifera_M_contig98697.22718.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=966bp|location=Sequence derived from alignment at M-pyrifera_M_contig98697:194..697- (Macrocystis pyrifera P11B4 male)
ATGCGTGAGCCAATCACACTGTCCTCCTCATCCTATGATCCTTCTGGTGC
TTCAGTTCAACCATCTATCCGACTCATGGTGTCTGTATTGGCTGGAAAGT
ACTTGCCAAAACCTGCAGGAGCATTGGAAGGTGAAGTGATTGATCCTTAC
GTTGAAGTACGCTTGTGTGGTTGTCCTGCGGACAATGCAACGAATTGCAC
TCCAGTCGTGGAGAATGACGGATTCAACCCAAGATGGGCTGATGGAAGTG
GAAAGAATGTGTTTACATACGACATTCGGCGCCCAGAAACTGCTCTGCTA
CTCTTTGTAGTTCGAGATTCTGACATGGGACCTGATGAGTTGGTTGGTTG
GTATGGTCTACGTCTTCCAATGGTACGCCAAGGATTACGCCTTGTTCCTC
TCCTTGATCAACATTCCGTCCCACTACAAGTGTCTACACTCTTTGTACAT
GTTTCTATTGATGAAATCGACAAGGATGGCAACATGCGTGAGCCAATCAC
ACTGTCCTCCTCATCCTATGATCCTTCTGGTGCTTCAGTTCAACCATCTA
TCCGACTCATGGTGTCTGTATTGGCTGGAAAGTACTTGCCAAAACCTGCA
GGAGCATTGGAAGGTGAAGTGATTGATCCTTACGTTGAAGTACGCTTGTG
TGGTTGTCCTGCGGACAATGCAACGAATTGCACTCCAGTCGTGGAGAATG
ACGGATTCAACCCAAGATGGGCTGATGGAAGTGGAAAGAATGTGTTTACA
TACGACATTCGGCGCCCAGAAACTGCTCTGCTACTCTTTGTAGTTCGAGA
TTCTGACATGGGACCTGATGAGTTGGTTGGTTGGTATGGTCTACGTCTTC
CAATGGTACGCCAAGGATTACGCCTTGTTCCTCTCCTTGATCAACATTCC
GTCCCACTACAAGTGTCTACACTCTTTGTACATGTTTCTATTGATGAAAT
CGACAAGGATGGCAAC
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