mRNA_M-pyrifera_M_contig98172.22604.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig98172.22604.1
Unique NamemRNA_M-pyrifera_M_contig98172.22604.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig98172.22604.1 vs. uniprot
Match: A0A835Z4E7_9STRA (Threonine synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z4E7_9STRA)

HSP 1 Score: 140 bits (354), Expect = 1.080e-36
Identity = 71/137 (51.82%), Postives = 96/137 (70.07%), Query Frame = 1
Query:    1 HGPTFAFKDVALQFLAELLEWKTTAEREAAGEEGAAAARKYTLLGATSGDTGSAALEAFKGKACVDGYVLYPKGRISKIQERQMTAIDDESLHAVAVDAPFDVCQRMVKEVLRSPELQEEFSFISINSINWARIMAQ 411
            HGPTFAFKDVALQFL  L E+     R A GE+      + T+LGATSGDTGSAA+   +GK  +D ++L+P+GR+S+IQERQMT ++D ++H V+V+  FD CQ +VK      E +E  S  ++NSINWAR++AQ
Sbjct:  107 HGPTFAFKDVALQFLGNLFEFFLE-RRRARGEQA-----RLTILGATSGDTGSAAIYGMRGKKHMDCFILFPEGRVSQIQERQMTTVEDTNIHCVSVEGSFDDCQAIVKAAFNDKEFRERVSLGAVNSINWARVLAQ 237          
BLAST of mRNA_M-pyrifera_M_contig98172.22604.1 vs. uniprot
Match: D8LN21_ECTSI (Threonine synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LN21_ECTSI)

HSP 1 Score: 137 bits (346), Expect = 8.120e-36
Identity = 71/137 (51.82%), Postives = 94/137 (68.61%), Query Frame = 1
Query:    1 HGPTFAFKDVALQFLAELLEWKTTAEREAAGEEGAAAARKYTLLGATSGDTGSAALEAFKGKACVDGYVLYPKGRISKIQERQMTAIDDESLHAVAVDAPFDVCQRMVKEVLRSPELQEEFSFISINSINWARIMAQ 411
            HGPTFAFKDVALQFL  L E+     R A GEE        T+LGATSGDTGSAA+   +GKA V+ ++++P+GR+S+IQERQMT + D ++H V+V+  FD CQ +VK        +EE    ++NSINWAR++AQ
Sbjct:  119 HGPTFAFKDVALQFLGNLFEYFLL-RRRAKGEEAG-----LTILGATSGDTGSAAIYGLRGKAHVNCFIMFPEGRVSEIQERQMTTVPDANIHCVSVEGSFDDCQDIVKAAFADKPFREEVKLGAVNSINWARVLAQ 249          
BLAST of mRNA_M-pyrifera_M_contig98172.22604.1 vs. uniprot
Match: A0A7S2SVW5_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2SVW5_9STRA)

HSP 1 Score: 133 bits (335), Expect = 8.800e-36
Identity = 68/137 (49.64%), Postives = 92/137 (67.15%), Query Frame = 1
Query:    1 HGPTFAFKDVALQFLAELLEWKTTAEREAAGEEGAAAARKYTLLGATSGDTGSAALEAFKGKACVDGYVLYPKGRISKIQERQMTAIDDESLHAVAVDAPFDVCQRMVKEVLRSPELQEEFSFISINSINWARIMAQ 411
            HGPTFAFKDVALQFL  L E+    ++   G E     R+ T++GATSGDTGSAA+   +GKA V+ ++LYPKGR+S IQERQM  + D ++H + V+  FD CQ +VK        ++E    ++NSINWAR++AQ
Sbjct:  110 HGPTFAFKDVALQFLGNLFEYFIGKKQ---GSE-----RRLTIMGATSGDTGSAAIYGLRGKANVNCFILYPKGRVSAIQERQMATVPDSNIHCLCVEGNFDDCQDIVKGAFMDKSFRDEVKLGAVNSINWARVLAQ 238          
BLAST of mRNA_M-pyrifera_M_contig98172.22604.1 vs. uniprot
Match: A0A1Y2CNN2_9FUNG (Tryptophan synthase beta subunit-like PLP-dependent enzyme n=1 Tax=Rhizoclosmatium globosum TaxID=329046 RepID=A0A1Y2CNN2_9FUNG)

HSP 1 Score: 134 bits (336), Expect = 1.010e-35
Identity = 70/138 (50.72%), Postives = 100/138 (72.46%), Query Frame = 1
Query:    1 HGPTFAFKDVALQFLAELLEWKTTAEREAAGEEGAAAARKYTLLGATSGDTGSAALEAFKGKACVDGYVLYPKGRISKIQERQMTAIDDESLHAVAVD-APFDVCQRMVKEVLRSPELQEEFSFISINSINWARIMAQ 411
            HGPTFAFKDVALQ L  L ++  +  + A+ +  A  + K  +LGATSGDTG AA+   +GK  VD ++L+PKGRIS +QE+QMT++ D ++H +AVD A FD CQ +VK ++ +PE + ++S  ++NSINWARI+AQ
Sbjct:  121 HGPTFAFKDVALQALGNLFDFFLSRPQPASSK--AERSDKIAVLGATSGDTGGAAIYGLRGKRNVDVFILHPKGRISPVQEQQMTSVLDPNVHNIAVDGATFDDCQEIVKVLMGNPEFKTKYSLAAVNSINWARILAQ 256          
BLAST of mRNA_M-pyrifera_M_contig98172.22604.1 vs. uniprot
Match: K8Z048_NANGC (Threonine synthase (Fragment) n=1 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=K8Z048_NANGC)

HSP 1 Score: 135 bits (340), Expect = 1.580e-35
Identity = 70/138 (50.72%), Postives = 98/138 (71.01%), Query Frame = 1
Query:    1 HGPTFAFKDVALQFLAELLEWKTTAEREAAGEEGAAAARKYTLLGATSGDTGSAALEAFKGKACVDGYVLYPKGRISKIQERQMTAIDDESLHAVAVD-APFDVCQRMVKEVLRSPELQEEFSFISINSINWARIMAQ 411
            HGPTFAFKDVALQFL  + E+    ++   G+       K T+LGATSGDTGSAA+E  +GK  V+ +VLYPKGR+S++QERQMT + D+++H +AV+ A FD CQ ++K   +  E +E+    ++NSINWAR++AQ
Sbjct:  192 HGPTFAFKDVALQFLGNVFEYFMAKKK---GDPDV----KLTVLGATSGDTGSAAIEGLRGKKNVNVFVLYPKGRVSEVQERQMTTVLDKNVHCIAVENATFDDCQSIIKSAFQDKEFREKVCLGAVNSINWARVLAQ 322          
BLAST of mRNA_M-pyrifera_M_contig98172.22604.1 vs. uniprot
Match: R4XF92_TAPDE (Threonine synthase n=1 Tax=Taphrina deformans (strain PYCC 5710 / ATCC 11124 / CBS 356.35 / IMI 108563 / JCM 9778 / NBRC 8474) TaxID=1097556 RepID=R4XF92_TAPDE)

HSP 1 Score: 137 bits (344), Expect = 2.470e-35
Identity = 68/137 (49.64%), Postives = 94/137 (68.61%), Query Frame = 1
Query:    1 HGPTFAFKDVALQFLAELLEWKTTAEREAAGEEGAAAARKYTLLGATSGDTGSAALEAFKGKACVDGYVLYPKGRISKIQERQMTAIDDESLHAVAVDAPFDVCQRMVKEVLRSPELQEEFSFISINSINWARIMAQ 411
            HGPTFAFKDVALQFL  L E+  + +     ++        T++GATSGDTGSAA+   +GK  +D ++L+P GR+S IQE QMT++ DE++H V+V+  FD CQ +VK +   PE   +FS  ++NSINWARI+AQ
Sbjct:  112 HGPTFAFKDVALQFLGNLFEFFLSRKNAKLSKD--EKRHHLTVIGATSGDTGSAAIYGLRGKKDIDVFILFPTGRVSPIQEAQMTSVPDENVHCVSVEGTFDDCQDIVKSLFNDPEFSTKFSLGAVNSINWARILAQ 246          
BLAST of mRNA_M-pyrifera_M_contig98172.22604.1 vs. uniprot
Match: A0A0L0G9B7_9EUKA (Threonine synthase n=1 Tax=Sphaeroforma arctica JP610 TaxID=667725 RepID=A0A0L0G9B7_9EUKA)

HSP 1 Score: 137 bits (344), Expect = 2.730e-35
Identity = 71/139 (51.08%), Postives = 94/139 (67.63%), Query Frame = 1
Query:    1 HGPTFAFKDVALQFLAELLEW--KTTAEREAAGEEGAAAARKYTLLGATSGDTGSAALEAFKGKACVDGYVLYPKGRISKIQERQMTAIDDESLHAVAVDAPFDVCQRMVKEVLRSPELQEEFSFISINSINWARIMAQ 411
            HGPTFAFKDVALQFL  L E+  K  AE+   GE      +  T+LGATSGDTGSAA+   +GK  +D ++L+PKGR++ +QERQMT + D ++H +AVD  FD CQ +VKE     +    +   ++NSINWARI+AQ
Sbjct:  106 HGPTFAFKDVALQFLGNLFEYFLKRKAEK---GES----VKPITVLGATSGDTGSAAIYGLRGKENIDVFILHPKGRVANVQERQMTTVLDSNVHNIAVDGSFDDCQAIVKECFSDKQFNTTYDLAAVNSINWARILAQ 237          
BLAST of mRNA_M-pyrifera_M_contig98172.22604.1 vs. uniprot
Match: A0A4P9ZEN1_9ASCO (Threonine synthase n=1 Tax=Metschnikowia bicuspidata TaxID=27322 RepID=A0A4P9ZEN1_9ASCO)

HSP 1 Score: 136 bits (343), Expect = 2.770e-35
Identity = 71/137 (51.82%), Postives = 95/137 (69.34%), Query Frame = 1
Query:    1 HGPTFAFKDVALQFLAELLEWKTTAEREAAGEEGAAAARKYTLLGATSGDTGSAALEAFKGKACVDGYVLYPKGRISKIQERQMTAIDDESLHAVAVDAPFDVCQRMVKEVLRSPELQEEFSFISINSINWARIMAQ 411
            HGPT+AFKDVALQF+  L E+     R  AG+EGA    K T++GATSGDTGSAA+   +GK  V  ++LYP GRIS IQE+QMT ++D ++H ++V   FD CQ +VK++    E  E+    ++NSINWARI+AQ
Sbjct:  112 HGPTYAFKDVALQFVGNLFEYFLV--RRNAGKEGADR-EKITVIGATSGDTGSAAIYGLRGKKDVSVFILYPTGRISPIQEKQMTTVEDPNVHTLSVKGTFDNCQDIVKQIFGDREFNEKHHVAAVNSINWARILAQ 245          
BLAST of mRNA_M-pyrifera_M_contig98172.22604.1 vs. uniprot
Match: UPI00096B8FD9 (threonine synthase n=1 Tax=Salaquimonas pukyongi TaxID=2712698 RepID=UPI00096B8FD9)

HSP 1 Score: 135 bits (341), Expect = 3.010e-35
Identity = 67/137 (48.91%), Postives = 94/137 (68.61%), Query Frame = 1
Query:    1 HGPTFAFKDVALQFLAELLEWKTTAEREAAGEEGAAAARKYTLLGATSGDTGSAALEAFKGKACVDGYVLYPKGRISKIQERQMTAIDDESLHAVAVDAPFDVCQRMVKEVLRSPELQEEFSFISINSINWARIMAQ 411
            HGPT AFKDVA+Q LA +++       E  GE       + T++GATSGDTG AA+EAF+G++ +D ++L+P GR+S +Q RQMT +DD ++H +AV   FD CQ +VK +   P+ ++E S   +NSINWARIMAQ
Sbjct:  105 HGPTMAFKDVAMQLLARMMDHVL----EQRGE-------RATIIGATSGDTGGAAIEAFRGRSNIDMFILFPDGRVSDVQRRQMTTVDDPAVHPLAVKGNFDDCQALVKALFNDPKFRDEVSLSGVNSINWARIMAQ 230          
BLAST of mRNA_M-pyrifera_M_contig98172.22604.1 vs. uniprot
Match: A0A3N4JZB7_9PEZI (Threonine synthase n=1 Tax=Choiromyces venosus 120613-1 TaxID=1336337 RepID=A0A3N4JZB7_9PEZI)

HSP 1 Score: 137 bits (344), Expect = 3.080e-35
Identity = 69/137 (50.36%), Postives = 94/137 (68.61%), Query Frame = 1
Query:    1 HGPTFAFKDVALQFLAELLEWKTTAEREAAGEEGAAAARKYTLLGATSGDTGSAALEAFKGKACVDGYVLYPKGRISKIQERQMTAIDDESLHAVAVDAPFDVCQRMVKEVLRSPELQEEFSFISINSINWARIMAQ 411
            HGPTFAFKDVALQF+  L E+    + + AG EG       T++GATSGDTGSAA+   +GK  V  ++L+PKGR+S IQE QMT + D+++H ++V   FD CQ +VK +   PEL +  +  ++NSINWARI+AQ
Sbjct:  115 HGPTFAFKDVALQFVGNLFEYFLVRKNKDAGGEGGKKRHHLTVIGATSGDTGSAAIYGLRGKKDVSVFILHPKGRVSPIQEAQMTTVLDKNVHNLSVVGTFDDCQDIVKALFSHPELSKSHNLGAVNSINWARILAQ 251          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig98172.22604.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835Z4E7_9STRA1.080e-3651.82Threonine synthase n=1 Tax=Tribonema minus TaxID=3... [more]
D8LN21_ECTSI8.120e-3651.82Threonine synthase n=1 Tax=Ectocarpus siliculosus ... [more]
A0A7S2SVW5_9STRA8.800e-3649.64Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A1Y2CNN2_9FUNG1.010e-3550.72Tryptophan synthase beta subunit-like PLP-dependen... [more]
K8Z048_NANGC1.580e-3550.72Threonine synthase (Fragment) n=1 Tax=Nannochlorop... [more]
R4XF92_TAPDE2.470e-3549.64Threonine synthase n=1 Tax=Taphrina deformans (str... [more]
A0A0L0G9B7_9EUKA2.730e-3551.08Threonine synthase n=1 Tax=Sphaeroforma arctica JP... [more]
A0A4P9ZEN1_9ASCO2.770e-3551.82Threonine synthase n=1 Tax=Metschnikowia bicuspida... [more]
UPI00096B8FD93.010e-3548.91threonine synthase n=1 Tax=Salaquimonas pukyongi T... [more]
A0A3N4JZB7_9PEZI3.080e-3550.36Threonine synthase n=1 Tax=Choiromyces venosus 120... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig98172contigM-pyrifera_M_contig98172:253..663 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score139.0
Seed ortholog evalue1.6e-30
Seed eggNOG ortholog2880.D8LN21
Preferred nameTHR4
KEGG rclassRC00017,RC00526
KEGG koko:K01733
KEGG ReactionR01466,R05086
KEGG Pathwayko00260,ko00750,ko01100,ko01110,ko01120,ko01230,map00260,map00750,map01100,map01110,map01120,map01230
KEGG ModuleM00018
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004795,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0006082,GO:0006520,GO:0006566,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0008652,GO:0009058,GO:0009066,GO:0009067,GO:0009088,GO:0009987,GO:0016053,GO:0016311,GO:0016829,GO:0016835,GO:0016838,GO:0019752,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0044237,GO:0044238,GO:0044249,GO:0044281,GO:0044283,GO:0044424,GO:0044464,GO:0046394,GO:0071704,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901607
EggNOG free text desc.2-oxobutyrate metabolic process
EggNOG OGsCOG0498@1,KOG2616@2759
EC4.2.3.1
COG Functional cat.E
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons1
Model size411
Cds size411
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816899.1063125-CDS-M-pyrifera_M_contig98172:252..6631622816899.1063125-CDS-M-pyrifera_M_contig98172:252..663Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig98172 253..663 +
1692278243.93776-CDS-M-pyrifera_M_contig98172:252..6631692278243.93776-CDS-M-pyrifera_M_contig98172:252..663Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig98172 253..663 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig98172.22604.1prot_M-pyrifera_M_contig98172.22604.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig98172 253..663 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig98172.22604.1

>prot_M-pyrifera_M_contig98172.22604.1 ID=prot_M-pyrifera_M_contig98172.22604.1|Name=mRNA_M-pyrifera_M_contig98172.22604.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=137bp
HGPTFAFKDVALQFLAELLEWKTTAEREAAGEEGAAAARKYTLLGATSGD
TGSAALEAFKGKACVDGYVLYPKGRISKIQERQMTAIDDESLHAVAVDAP
FDVCQRMVKEVLRSPELQEEFSFISINSINWARIMAQ
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mRNA from alignment at M-pyrifera_M_contig98172:253..663+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig98172.22604.1 ID=mRNA_M-pyrifera_M_contig98172.22604.1|Name=mRNA_M-pyrifera_M_contig98172.22604.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=411bp|location=Sequence derived from alignment at M-pyrifera_M_contig98172:253..663+ (Macrocystis pyrifera P11B4 male)
CACGGGCCCACGTTCGCCTTCAAGGACGTGGCACTGCAGTTCCTCGCCGA GCTGCTCGAGTGGAAGACCACTGCCGAGAGGGAGGCTGCTGGCGAGGAGG GGGCTGCTGCGGCAAGGAAGTACACGCTGCTCGGCGCCACCTCGGGCGAC ACTGGCAGCGCTGCATTGGAGGCCTTCAAGGGGAAGGCGTGCGTGGACGG ATACGTGCTGTACCCCAAGGGCAGGATCAGCAAGATCCAGGAGCGCCAGA TGACTGCCATCGACGACGAGAGCCTCCACGCCGTGGCCGTCGACGCGCCG TTCGACGTTTGCCAGCGCATGGTGAAGGAGGTGCTCCGCAGCCCAGAGCT GCAGGAAGAGTTCAGCTTCATCTCCATTAACAGTATCAACTGGGCCCGCA TCATGGCACAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig98172:253..663+

>mRNA_M-pyrifera_M_contig98172.22604.1 ID=mRNA_M-pyrifera_M_contig98172.22604.1|Name=mRNA_M-pyrifera_M_contig98172.22604.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=822bp|location=Sequence derived from alignment at M-pyrifera_M_contig98172:253..663+ (Macrocystis pyrifera P11B4 male)
CACGGGCCCACGTTCGCCTTCAAGGACGTGGCACTGCAGTTCCTCGCCGA
GCTGCTCGAGTGGAAGACCACTGCCGAGAGGGAGGCTGCTGGCGAGGAGG
GGGCTGCTGCGGCAAGGAAGTACACGCTGCTCGGCGCCACCTCGGGCGAC
ACTGGCAGCGCTGCATTGGAGGCCTTCAAGGGGAAGGCGTGCGTGGACGG
ATACGTGCTGTACCCCAAGGGCAGGATCAGCAAGATCCAGGAGCGCCAGA
TGACTGCCATCGACGACGAGAGCCTCCACGCCGTGGCCGTCGACGCGCCG
TTCGACGTTTGCCAGCGCATGGTGAAGGAGGTGCTCCGCAGCCCAGAGCT
GCAGGAAGAGTTCAGCTTCATCTCCATTAACAGTATCAACTGGGCCCGCA
TCATGGCACAGCACGGGCCCACGTTCGCCTTCAAGGACGTGGCACTGCAG
TTCCTCGCCGAGCTGCTCGAGTGGAAGACCACTGCCGAGAGGGAGGCTGC
TGGCGAGGAGGGGGCTGCTGCGGCAAGGAAGTACACGCTGCTCGGCGCCA
CCTCGGGCGACACTGGCAGCGCTGCATTGGAGGCCTTCAAGGGGAAGGCG
TGCGTGGACGGATACGTGCTGTACCCCAAGGGCAGGATCAGCAAGATCCA
GGAGCGCCAGATGACTGCCATCGACGACGAGAGCCTCCACGCCGTGGCCG
TCGACGCGCCGTTCGACGTTTGCCAGCGCATGGTGAAGGAGGTGCTCCGC
AGCCCAGAGCTGCAGGAAGAGTTCAGCTTCATCTCCATTAACAGTATCAA
CTGGGCCCGCATCATGGCACAG
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