mRNA_M-pyrifera_M_contig97999.22566.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig97999.22566.1
Unique NamemRNA_M-pyrifera_M_contig97999.22566.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig97999.22566.1 vs. uniprot
Match: A0A518ACZ2_9PLAN (Putative sugar kinase YdjH n=8 Tax=Gimesia TaxID=1649453 RepID=A0A518ACZ2_9PLAN)

HSP 1 Score: 326 bits (836), Expect = 1.760e-110
Identity = 160/173 (92.49%), Postives = 167/173 (96.53%), Query Frame = 1
Query:    1 ELSPENVARMFEVAREAGVITVLDVVTPKAADYWSMLKPVLPLSDYFLPNNDEGELITGETDPLKQAEAFREAGANSVIITCGGQGSLLLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEEASPEDCLRYGSALGHSCVRATGATAGIFTRSELNQFVSAHELRVETL 519
            ELSPENVARMFEVAR+AGVITVLDVVTPK ADYW MLKPVLPLSDYFLPNNDEGELITGE DPLKQA+AFR+AGANSVIITCGGQGS+LLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEE SPE CLRYGSALGHSCVRATGATAGIFTR ELN+FV+AH+LRVETL
Sbjct:  148 ELSPENVARMFEVARDAGVITVLDVVTPKPADYWQMLKPVLPLSDYFLPNNDEGELITGEADPLKQAQAFRDAGANSVIITCGGQGSILLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEEGSPEACLRYGSALGHSCVRATGATAGIFTRDELNEFVAAHDLRVETL 320          
BLAST of mRNA_M-pyrifera_M_contig97999.22566.1 vs. uniprot
Match: A0A517VK48_9PLAN (Putative sugar kinase YdjH n=10 Tax=Planctomycetaceae TaxID=126 RepID=A0A517VK48_9PLAN)

HSP 1 Score: 296 bits (758), Expect = 1.210e-98
Identity = 141/173 (81.50%), Postives = 161/173 (93.06%), Query Frame = 1
Query:    1 ELSPENVARMFEVAREAGVITVLDVVTPKAADYWSMLKPVLPLSDYFLPNNDEGELITGETDPLKQAEAFREAGANSVIITCGGQGSLLLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEEASPEDCLRYGSALGHSCVRATGATAGIFTRSELNQFVSAHELRVETL 519
            ELSPENVA+MF +A+EAGV TVLDVVTPK ADYW ML+PVLP SDYFLPNNDEGELITGE+DPL+QA AFR+AGA  VIITCGG+GS+LLDAER+++SEIYPV+LVDGTGSGDAFVAGFIHGLLE ASPEDCL++GSALGHSCVRATGATAGIFTR EL++FV AH+L+V T+
Sbjct:  148 ELSPENVAKMFRMAKEAGVTTVLDVVTPKPADYWKMLEPVLPYSDYFLPNNDEGELITGESDPLEQARAFRKAGAKHVIITCGGEGSILLDAERTIQSEIYPVNLVDGTGSGDAFVAGFIHGLLEGASPEDCLKFGSALGHSCVRATGATAGIFTRGELDEFVGAHDLQVRTI 320          
BLAST of mRNA_M-pyrifera_M_contig97999.22566.1 vs. uniprot
Match: A0A518IIQ5_9PLAN (Putative sugar kinase YdjH n=2 Tax=Gimesia TaxID=1649453 RepID=A0A518IIQ5_9PLAN)

HSP 1 Score: 286 bits (732), Expect = 1.070e-94
Identity = 135/173 (78.03%), Postives = 157/173 (90.75%), Query Frame = 1
Query:    1 ELSPENVARMFEVAREAGVITVLDVVTPKAADYWSMLKPVLPLSDYFLPNNDEGELITGETDPLKQAEAFREAGANSVIITCGGQGSLLLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEEASPEDCLRYGSALGHSCVRATGATAGIFTRSELNQFVSAHELRVETL 519
            ELSP+NVA+MF +A+EAGV TVLDVVTP+  DYW MLKPVLPLSDYFLPNNDEGELITGE+DPL QA AF+ AGA +VIITCGG+GS+L+D ++++ S +YPV LVDGTGSGDAFVAGFIHGLLE  SPE+CLR+GSALGHSCVRATGATAGIFTRSELNQF+ +HEL+V+TL
Sbjct:  148 ELSPDNVAQMFRIAKEAGVTTVLDVVTPQPDDYWKMLKPVLPLSDYFLPNNDEGELITGESDPLAQARAFKAAGAKTVIITCGGEGSILMDDQQTIHSAVYPVHLVDGTGSGDAFVAGFIHGLLEGVSPEECLRFGSALGHSCVRATGATAGIFTRSELNQFLGSHELQVKTL 320          
BLAST of mRNA_M-pyrifera_M_contig97999.22566.1 vs. uniprot
Match: A0A517WRX1_9PLAN (Putative sugar kinase YdjH n=2 Tax=Gimesia aquarii TaxID=2527964 RepID=A0A517WRX1_9PLAN)

HSP 1 Score: 283 bits (725), Expect = 1.240e-93
Identity = 132/173 (76.30%), Postives = 157/173 (90.75%), Query Frame = 1
Query:    1 ELSPENVARMFEVAREAGVITVLDVVTPKAADYWSMLKPVLPLSDYFLPNNDEGELITGETDPLKQAEAFREAGANSVIITCGGQGSLLLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEEASPEDCLRYGSALGHSCVRATGATAGIFTRSELNQFVSAHELRVETL 519
            +LSPENV  MF +A++AGV TVLDVVTPK  DYW MLKPVLPLSDYFLPNNDEGELITG++DP++QA  F+ AGAN+VIITCG +GS+L+   ++++SEIYPV+LVDGTGSGDAFVAGFIHGLLEE SPE+CL++GSALGHSCVRATGATAGIFTR+ELNQFVS+HEL +ET+
Sbjct:  148 KLSPENVTNMFHIAKKAGVTTVLDVVTPKQDDYWKMLKPVLPLSDYFLPNNDEGELITGKSDPIEQAREFKAAGANTVIITCGSEGSILMSENKTIQSEIYPVNLVDGTGSGDAFVAGFIHGLLEEGSPEECLQFGSALGHSCVRATGATAGIFTRNELNQFVSSHELPIETV 320          
BLAST of mRNA_M-pyrifera_M_contig97999.22566.1 vs. uniprot
Match: UPI001420DFAD (carbohydrate kinase family protein n=1 Tax=Rubinisphaera sp. JC658 TaxID=2713176 RepID=UPI001420DFAD)

HSP 1 Score: 205 bits (522), Expect = 6.670e-63
Identity = 100/171 (58.48%), Postives = 126/171 (73.68%), Query Frame = 1
Query:    1 ELSPENVARMFEVAREAGVITVLDVVTPKAADYWSMLKPVLPLSDYFLPNNDEGELITGETDPLKQAEAFREAGANSVIITCGGQGSLLLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEEASPEDCLRYGSALGHSCVRATGATAGIFTRSELNQFVSAHELRVE 513
            EL+ ++VA  F  ARE GV T+LDVV P A DYWS +  VLP +D FLPN DE +LITG TDP +QA+ FR+AGA +V ITCG  G++L  A  + RS  + V +VDGTGSGDAF AGFIHGLL +A PE CL++GSALG SCV+A GAT G+F+  EL QF +A++L +E
Sbjct:  148 ELAADHVAAAFRTARELGVTTILDVVVPAAGDYWSRVAAVLPFTDVFLPNTDEAKLITGLTDPTEQAQKFRDAGAKTVAITCGDTGAVLQSAAGNWRSGRFDVAVVDGTGSGDAFAAGFIHGLLADADPETCLQWGSALGASCVQAVGATTGVFSDGELRQFCAANQLAIE 318          
BLAST of mRNA_M-pyrifera_M_contig97999.22566.1 vs. uniprot
Match: A0A2D6SRS2_9PLAN (PfkB domain-containing protein n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A2D6SRS2_9PLAN)

HSP 1 Score: 205 bits (521), Expect = 9.440e-63
Identity = 104/170 (61.18%), Postives = 129/170 (75.88%), Query Frame = 1
Query:    4 LSPENVARMFEVAREAGVITVLDVVTPKAADYWSMLKPVLPLSDYFLPNNDEGELITGETDPLKQAEAFREAGANSVIITCGGQGSLLLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEEASPEDCLRYGSALGHSCVRATGATAGIFTRSELNQFVSAHELRVE 513
            LSP NVA +F  AR AGV TVLDVV P  AD   +L+PVLPL+D FLPN+ E  L+TG +DPL+QA  F++AGA +V +TCG +G +L+ A+ SLRS +Y VD+VDGTGSGDAFVAG+I+GLL EA  E CLRYG+ALG SCV ATGA+ GI +R EL +FV+   L VE
Sbjct:  149 LSPANVAALFLRARAAGVRTVLDVVLPGPADVDELLRPVLPLTDVFLPNDSEAALLTGLSDPLEQARFFQDAGAATVGVTCGSEGVVLVSADVSLRSGVYKVDVVDGTGSGDAFVAGYIYGLLREADVEQCLRYGAALGASCVTATGASTGILSRDELEEFVATRTLAVE 318          
BLAST of mRNA_M-pyrifera_M_contig97999.22566.1 vs. uniprot
Match: A0A836RIN2_9PLAN (Carbohydrate kinase family protein n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A836RIN2_9PLAN)

HSP 1 Score: 203 bits (516), Expect = 5.510e-62
Identity = 100/169 (59.17%), Postives = 124/169 (73.37%), Query Frame = 1
Query:   13 ENVARMFEVAREAGVITVLDVVTPKAADYWSMLKPVLPLSDYFLPNNDEGELITGETDPLKQAEAFREAGANSVIITCGGQGSLLLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEEASPEDCLRYGSALGHSCVRATGATAGIFTRSELNQFVSAHELRVETL 519
            ENVAR+FE+ARE GV TVLDVV P  ADYW  L+PVLP +D FLPN+DE  +ITGETDP++QAE F E GA +V+ITCG +G++ L     L+++   V+ VDGTGSGDAF AGFI+GLL   +  +CLR GSALG SCVR TGATAG+ T  EL  FV    L++ +L
Sbjct:  152 ENVARLFEIAREKGVTTVLDVVIPGEADYWPKLRPVLPHTDVFLPNDDEARIITGETDPVRQAERFHELGAETVVITCGERGAIPLSDTALLQADADRVEFVDGTGSGDAFDAGFIYGLLNGKTEVECLRLGSALGASCVRETGATAGVLTEPELRTFVDERPLKITSL 320          
BLAST of mRNA_M-pyrifera_M_contig97999.22566.1 vs. uniprot
Match: UPI0019C36478 (Carbohydrate kinase family protein n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=UPI0019C36478)

HSP 1 Score: 200 bits (508), Expect = 9.710e-62
Identity = 94/166 (56.63%), Postives = 124/166 (74.70%), Query Frame = 1
Query:   16 NVARMFEVAREAGVITVLDVVTPKAADYWSMLKPVLPLSDYFLPNNDEGELITGETDPLKQAEAFREAGANSVIITCGGQGSLLLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEEASPEDCLRYGSALGHSCVRATGATAGIFTRSELNQFVSAHELRVE 513
            ++  +F+ AR+AG+ T+LDVV P   DYW+ L+PVL L+D F+PN DE  L+TGETDP +QAEAFR AG  + IIT GG G ++L  E   ++E + V  VDGTGSGDAFV+G+I GLL  AS  DC+R GSA+G SCVR+TGAT G+F  +ELN+FV+AH L++E
Sbjct:   79 SIVPLFQAARDAGIPTLLDVVVPGPGDYWARLRPVLALTDVFMPNEDESVLLTGETDPWRQAEAFRSAGVRTAIITRGGHGCVILSDEGRFQAEAHRVPFVDGTGSGDAFVSGYISGLLRGASTLDCVRLGSAMGASCVRSTGATTGVFNGAELNEFVAAHPLKIE 244          
BLAST of mRNA_M-pyrifera_M_contig97999.22566.1 vs. uniprot
Match: A0A2E0SQ86_9PLAN (Carbohydrate kinase n=1 Tax=Planctomyces sp. TaxID=37635 RepID=A0A2E0SQ86_9PLAN)

HSP 1 Score: 200 bits (509), Expect = 6.090e-61
Identity = 92/172 (53.49%), Postives = 124/172 (72.09%), Query Frame = 1
Query:    4 LSPENVARMFEVAREAGVITVLDVVTPKAADYWSMLKPVLPLSDYFLPNNDEGELITGETDPLKQAEAFREAGANSVIITCGGQGSLLLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEEASPEDCLRYGSALGHSCVRATGATAGIFTRSELNQFVSAHELRVETL 519
            LS +NV  +F++A+EAGV TVLDVVTP+  DYW+ +K VLP +DYF+PN+DE EL+TG +DP++QA    +AG  +++ITCG +G++++  +   R+  YPVD VDGTGSGDAFVAGF+HGLL+  S  DC+  GSA+G S VRA GAT  +FT  EL  F+  H L    L
Sbjct:  149 LSQQNVINVFKLAKEAGVTTVLDVVTPQQRDYWNDIKDVLPWTDYFMPNDDEAELLTGLSDPVEQARKMHDAGVGTILITCGDEGTVVMSKDDRFRTRCYPVDFVDGTGSGDAFVAGFMHGLLDHKSLRDCVTCGSAMGASAVRAVGATTSVFTADELADFIDRHPLEATPL 320          
BLAST of mRNA_M-pyrifera_M_contig97999.22566.1 vs. uniprot
Match: A0A518B8G4_9BACT (Putative sugar kinase YdjH n=1 Tax=Planctomycetes bacterium Pan216 TaxID=2527975 RepID=A0A518B8G4_9BACT)

HSP 1 Score: 198 bits (503), Expect = 4.750e-60
Identity = 97/166 (58.43%), Postives = 123/166 (74.10%), Query Frame = 1
Query:   16 NVARMFEVAREAGVITVLDVVTPKAADYWSMLKPVLPLSDYFLPNNDEGELITGETDPLKQAEAFREAGANSVIITCGGQGSLLLDAERSLRSEIYPVDLVDGTGSGDAFVAGFIHGLLEEASPEDCLRYGSALGHSCVRATGATAGIFTRSELNQFVSAHELRVE 513
             +A++FE AR AG +TVLDVVTP   DY+  LK +LP +D FLPN+DEG+ ITG  DPL+QA AFREAGA  V+IT G +GS+LL      RS+ +  D+VDG GSGDAF AGFI+GLL+   PE+CLRYG+ALG S VRA GAT G+FTR E  +F+ A+ L++E
Sbjct:  152 ELAKVFETARNAGAVTVLDVVTPGPGDYFGALKELLPSTDVFLPNHDEGKFITGIEDPLEQAHAFREAGAGCVVITQGSEGSILLTDNGRWRSDCFSFDVVDGVGSGDAFDAGFIYGLLQGGGPEECLRYGTALGGSAVRAAGATDGVFTREEAIKFLEANPLKIE 317          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig97999.22566.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A518ACZ2_9PLAN1.760e-11092.49Putative sugar kinase YdjH n=8 Tax=Gimesia TaxID=1... [more]
A0A517VK48_9PLAN1.210e-9881.50Putative sugar kinase YdjH n=10 Tax=Planctomycetac... [more]
A0A518IIQ5_9PLAN1.070e-9478.03Putative sugar kinase YdjH n=2 Tax=Gimesia TaxID=1... [more]
A0A517WRX1_9PLAN1.240e-9376.30Putative sugar kinase YdjH n=2 Tax=Gimesia aquarii... [more]
UPI001420DFAD6.670e-6358.48carbohydrate kinase family protein n=1 Tax=Rubinis... [more]
A0A2D6SRS2_9PLAN9.440e-6361.18PfkB domain-containing protein n=1 Tax=Planctomyce... [more]
A0A836RIN2_9PLAN5.510e-6259.17Carbohydrate kinase family protein n=1 Tax=Plancto... [more]
UPI0019C364789.710e-6256.63Carbohydrate kinase family protein n=1 Tax=Plancto... [more]
A0A2E0SQ86_9PLAN6.090e-6153.49Carbohydrate kinase n=1 Tax=Planctomyces sp. TaxID... [more]
A0A518B8G4_9BACT4.750e-6058.43Putative sugar kinase YdjH n=1 Tax=Planctomycetes ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig97999contigM-pyrifera_M_contig97999:3..524 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score271.9
Seed ortholog evalue1.9e-70
Seed eggNOG ortholog344747.PM8797T_10259
Preferred nameydjH
KEGG rclassRC00002,RC00017
KEGG koko:K00852
KEGG ReactionR01051,R02750
KEGG Pathwayko00030,map00030
Hectar predicted targeting categoryother localisation
EggNOG free text desc.pfkB family carbohydrate kinase
EggNOG OGs2IX8I@203682,COG0524@1,COG0524@2
EC2.7.1.15
COG Functional cat.G
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size522
Cds size495
Stop1
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816897.1873572-UTR-M-pyrifera_M_contig97999:2..291622816897.1873572-UTR-M-pyrifera_M_contig97999:2..29Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig97999 3..29 +
1692278242.860304-UTR-M-pyrifera_M_contig97999:2..291692278242.860304-UTR-M-pyrifera_M_contig97999:2..29Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig97999 3..29 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816897.1993072-CDS-M-pyrifera_M_contig97999:29..5241622816897.1993072-CDS-M-pyrifera_M_contig97999:29..524Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig97999 30..524 +
1692278242.8713222-CDS-M-pyrifera_M_contig97999:29..5241692278242.8713222-CDS-M-pyrifera_M_contig97999:29..524Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig97999 30..524 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig97999.22566.1prot_M-pyrifera_M_contig97999.22566.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig97999 30..524 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig97999.22566.1

>prot_M-pyrifera_M_contig97999.22566.1 ID=prot_M-pyrifera_M_contig97999.22566.1|Name=mRNA_M-pyrifera_M_contig97999.22566.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=165bp
MFEVAREAGVITVLDVVTPKAADYWSMLKPVLPLSDYFLPNNDEGELITG
ETDPLKQAEAFREAGANSVIITCGGQGSLLLDAERSLRSEIYPVDLVDGT
GSGDAFVAGFIHGLLEEASPEDCLRYGSALGHSCVRATGATAGIFTRSEL
NQFVSAHELRVETL*
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mRNA from alignment at M-pyrifera_M_contig97999:3..524+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig97999.22566.1 ID=mRNA_M-pyrifera_M_contig97999.22566.1|Name=mRNA_M-pyrifera_M_contig97999.22566.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=522bp|location=Sequence derived from alignment at M-pyrifera_M_contig97999:3..524+ (Macrocystis pyrifera P11B4 male)
GAGCTCTCGCCGGAGAATGTCGCCCGCATGTTCGAGGTGGCCCGGGAAGC GGGAGTGATTACGGTGCTGGATGTGGTGACGCCAAAGGCTGCAGATTACT GGTCAATGTTGAAGCCGGTTCTGCCGTTAAGCGATTATTTCCTCCCTAAT AATGATGAGGGGGAACTGATCACAGGAGAGACAGACCCGCTGAAACAGGC CGAGGCGTTCCGGGAAGCGGGAGCGAACTCGGTGATCATCACCTGTGGCG GCCAGGGGAGTCTGCTGCTGGATGCCGAGCGTTCACTGCGGTCAGAGATT TATCCGGTCGACCTGGTGGATGGGACAGGGAGCGGAGACGCGTTTGTCGC CGGTTTCATTCACGGGCTTCTGGAAGAGGCGAGCCCGGAAGACTGTCTGC GTTATGGATCGGCGCTGGGGCACAGCTGTGTGCGTGCCACCGGCGCGACC GCGGGCATCTTCACACGCAGCGAGCTGAACCAGTTCGTGTCGGCGCATGA ACTGCGTGTAGAAACCCTGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig97999:3..524+

>mRNA_M-pyrifera_M_contig97999.22566.1 ID=mRNA_M-pyrifera_M_contig97999.22566.1|Name=mRNA_M-pyrifera_M_contig97999.22566.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=990bp|location=Sequence derived from alignment at M-pyrifera_M_contig97999:3..524+ (Macrocystis pyrifera P11B4 male)
ATGTTCGAGGTGGCCCGGGAAGCGGGAGTGATTACGGTGCTGGATGTGGT
GACGCCAAAGGCTGCAGATTACTGGTCAATGTTGAAGCCGGTTCTGCCGT
TAAGCGATTATTTCCTCCCTAATAATGATGAGGGGGAACTGATCACAGGA
GAGACAGACCCGCTGAAACAGGCCGAGGCGTTCCGGGAAGCGGGAGCGAA
CTCGGTGATCATCACCTGTGGCGGCCAGGGGAGTCTGCTGCTGGATGCCG
AGCGTTCACTGCGGTCAGAGATTTATCCGGTCGACCTGGTGGATGGGACA
GGGAGCGGAGACGCGTTTGTCGCCGGTTTCATTCACGGGCTTCTGGAAGA
GGCGAGCCCGGAAGACTGTCTGCGTTATGGATCGGCGCTGGGGCACAGCT
GTGTGCGTGCCACCGGCGCGACCGCGGGCATCTTCACACGCAGCGAGCTG
AACCAGTTCGTGTCGGCGCATGAACTGCGTGTAGAAACCCTGTAAATGTT
CGAGGTGGCCCGGGAAGCGGGAGTGATTACGGTGCTGGATGTGGTGACGC
CAAAGGCTGCAGATTACTGGTCAATGTTGAAGCCGGTTCTGCCGTTAAGC
GATTATTTCCTCCCTAATAATGATGAGGGGGAACTGATCACAGGAGAGAC
AGACCCGCTGAAACAGGCCGAGGCGTTCCGGGAAGCGGGAGCGAACTCGG
TGATCATCACCTGTGGCGGCCAGGGGAGTCTGCTGCTGGATGCCGAGCGT
TCACTGCGGTCAGAGATTTATCCGGTCGACCTGGTGGATGGGACAGGGAG
CGGAGACGCGTTTGTCGCCGGTTTCATTCACGGGCTTCTGGAAGAGGCGA
GCCCGGAAGACTGTCTGCGTTATGGATCGGCGCTGGGGCACAGCTGTGTG
CGTGCCACCGGCGCGACCGCGGGCATCTTCACACGCAGCGAGCTGAACCA
GTTCGTGTCGGCGCATGAACTGCGTGTAGAAACCCTGTAA
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