mRNA_M-pyrifera_M_contig949.21890.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig949.21890.1
Unique NamemRNA_M-pyrifera_M_contig949.21890.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig949.21890.1 vs. uniprot
Match: A0A6H5KTL6_9PHAE (Fructose-bisphosphatase n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KTL6_9PHAE)

HSP 1 Score: 189 bits (480), Expect = 2.080e-55
Identity = 89/113 (78.76%), Postives = 103/113 (91.15%), Query Frame = 2
Query:    2 EELCLLRTLQPGRNLVGAGYAMYSSSCELVLAVQGQGVHGFTLDAHVGEFILTRPYIKIPKRGSIYSLDEGRRESWTKPLREHVDKLQRGKGESGKKYSYRLVGSAVADVHRT 340
            EELCLLRTLQPG+NL+GAGYA+YS SCELVLAV+GQGVHGFTLD+ VGEFILT+PY++IP RGS YSLDE RR +W K L+EH+DKL+ G+GESGKK+ YRLVGSAVADVHRT
Sbjct:  235 EELCLLRTLQPGKNLIGAGYAIYSGSCELVLAVKGQGVHGFTLDSSVGEFILTKPYMRIPSRGSGYSLDESRRGTWPKALQEHIDKLRSGEGESGKKFGYRLVGSAVADVHRT 347          
BLAST of mRNA_M-pyrifera_M_contig949.21890.1 vs. uniprot
Match: X2DAQ5_9ALVE (Fructose-bisphosphatase n=1 Tax=Chromera velia TaxID=505693 RepID=X2DAQ5_9ALVE)

HSP 1 Score: 139 bits (351), Expect = 1.810e-37
Identity = 66/112 (58.93%), Postives = 83/112 (74.11%), Query Frame = 2
Query:    5 ELCLLRTLQPGRNLVGAGYAMYSSSCELVLAVQGQGVHGFTLDAHVGEFILTRPYIKIPKRGSIYSLDEGRRESWTKPLREHVDKLQRGKGESGKKYSYRLVGSAVADVHRT 340
            E CL+RTLQPG NLV AGY +YSSSC L +   G GV GFTLD  VGEF+LT P +KIP RG IYS +EG    W + L+++VD +++G+GE+GKKY+ R +GS V DVHRT
Sbjct:  223 ERCLIRTLQPGSNLVAAGYCLYSSSCHLFVTF-GNGVQGFTLDPEVGEFVLTHPDVKIPSRGKIYSFNEGNSPDWEEGLQKYVDDVKKGQGETGKKYTSRYIGSMVGDVHRT 333          
BLAST of mRNA_M-pyrifera_M_contig949.21890.1 vs. uniprot
Match: W7TEP4_9STRA (Fructose-bisphosphatase n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TEP4_9STRA)

HSP 1 Score: 139 bits (350), Expect = 7.900e-37
Identity = 65/110 (59.09%), Postives = 82/110 (74.55%), Query Frame = 2
Query:   11 CLLRTLQPGRNLVGAGYAMYSSSCELVLAVQGQGVHGFTLDAHVGEFILTRPYIKIPKRGSIYSLDEGRRESWTKPLREHVDKLQRGKGESGKKYSYRLVGSAVADVHRT 340
            CL+ TLQPG NLV AGY +YSSS  LV  + G+GV+GFT D H+GEF+LT P I+IP+RG IYS +E  R  W  P++E+V  LQ G+G+SGK+YS R +GS V DVHRT
Sbjct:  253 CLMSTLQPGTNLVAAGYCLYSSSAHLVFTL-GRGVNGFTYDTHIGEFVLTHPNIRIPERGQIYSFNEANRWDWDSPMQEYVTSLQMGQGQSGKRYSSRYIGSMVGDVHRT 361          
BLAST of mRNA_M-pyrifera_M_contig949.21890.1 vs. uniprot
Match: A0A813CI76_9DINO (Fructose-bisphosphatase (Fragment) n=2 Tax=unclassified Symbiodinium TaxID=279888 RepID=A0A813CI76_9DINO)

HSP 1 Score: 133 bits (334), Expect = 1.440e-36
Identity = 63/110 (57.27%), Postives = 80/110 (72.73%), Query Frame = 2
Query:   11 CLLRTLQPGRNLVGAGYAMYSSSCELVLAVQGQGVHGFTLDAHVGEFILTRPYIKIPKRGSIYSLDEGRRESWTKPLREHVDKLQRGKGESGKKYSYRLVGSAVADVHRT 340
            CL  TLQPG+NLV AGY +YS S ELV  + G+GV GFTLD  +GE++LTRP IKIP RG IYS +E   + W +P ++++  +Q GKGE+G KYS R +GS V DVHRT
Sbjct:   60 CLAATLQPGKNLVAAGYVLYSCSTELVFTI-GRGVVGFTLDNSIGEYVLTRPSIKIPARGKIYSCNEANIDKWDEPFQQYIKDIQMGKGETGDKYSLRYIGSMVGDVHRT 168          
BLAST of mRNA_M-pyrifera_M_contig949.21890.1 vs. uniprot
Match: A0A7S2V7Q3_9STRA (Fructose-bisphosphatase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V7Q3_9STRA)

HSP 1 Score: 136 bits (343), Expect = 1.870e-36
Identity = 64/113 (56.64%), Postives = 84/113 (74.34%), Query Frame = 2
Query:    2 EELCLLRTLQPGRNLVGAGYAMYSSSCELVLAVQGQGVHGFTLDAHVGEFILTRPYIKIPKRGSIYSLDEGRRESWTKPLREHVDKLQRGKGESGKKYSYRLVGSAVADVHRT 340
            E  CL++TLQPG++LV AGY MYSSS  LV  + G G+HGFTLD  +GEF+LT P +++PKRG IYS +E     W +PL+++VD L+ G GESG++YS R +GS V DVHRT
Sbjct:  201 EMQCLMQTLQPGKSLVAAGYCMYSSSTILVFTM-GNGLHGFTLDPQIGEFVLTHPNMRVPKRGKIYSFNEANYNDWDEPLQKYVDALKTGTGESGERYSSRYIGSMVGDVHRT 312          
BLAST of mRNA_M-pyrifera_M_contig949.21890.1 vs. uniprot
Match: A0A4D9D3H2_9STRA (Uncharacterized protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D3H2_9STRA)

HSP 1 Score: 139 bits (350), Expect = 4.370e-36
Identity = 65/110 (59.09%), Postives = 82/110 (74.55%), Query Frame = 2
Query:   11 CLLRTLQPGRNLVGAGYAMYSSSCELVLAVQGQGVHGFTLDAHVGEFILTRPYIKIPKRGSIYSLDEGRRESWTKPLREHVDKLQRGKGESGKKYSYRLVGSAVADVHRT 340
            CL+ TLQPG NLV AGY +YSSS  LV  + G+GV+GFT D H+GEF+LT P I+IP+RG IYS +E  R  W  P++E+V  LQ G+G+SGK+YS R +GS V DVHRT
Sbjct:  504 CLMSTLQPGTNLVAAGYCLYSSSAHLVFTL-GRGVNGFTYDTHIGEFVLTHPNIRIPERGQIYSFNEANRWDWDSPMQEYVTSLQMGQGQSGKRYSSRYIGSMVGDVHRT 612          
BLAST of mRNA_M-pyrifera_M_contig949.21890.1 vs. uniprot
Match: A0A7S1L2B5_ALECA (Fructose-bisphosphatase (Fragment) n=1 Tax=Alexandrium catenella TaxID=2925 RepID=A0A7S1L2B5_ALECA)

HSP 1 Score: 132 bits (333), Expect = 5.030e-36
Identity = 64/110 (58.18%), Postives = 82/110 (74.55%), Query Frame = 2
Query:   11 CLLRTLQPGRNLVGAGYAMYSSSCELVLAVQGQGVHGFTLDAHVGEFILTRPYIKIPKRGSIYSLDEGRRESWTKPLREHVDKLQRGKGESGKKYSYRLVGSAVADVHRT 340
            CL +TLQPG+NLV AGY  YSSS ELV+   G GV GFTLD  VG+F LTRP I+IPKRG+IYS ++   + W KP++++++ +Q G GE+GK YS R +GS V DVHRT
Sbjct:  141 CLAQTLQPGKNLVAAGYCFYSSSTELVVTF-GHGVAGFTLDQDVGDFKLTRPSIEIPKRGNIYSANQANFKQWDKPMQDYIEAIQDGAGETGKPYSLRYIGSMVGDVHRT 249          
BLAST of mRNA_M-pyrifera_M_contig949.21890.1 vs. uniprot
Match: A0A836CD45_9STRA (Fructose-bisphosphatase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CD45_9STRA)

HSP 1 Score: 135 bits (340), Expect = 1.120e-35
Identity = 65/110 (59.09%), Postives = 80/110 (72.73%), Query Frame = 2
Query:   11 CLLRTLQPGRNLVGAGYAMYSSSCELVLAVQGQGVHGFTLDAHVGEFILTRPYIKIPKRGSIYSLDEGRRESWTKPLREHVDKLQRGKGESGKKYSYRLVGSAVADVHRT 340
            CL  TLQPG  LV AGY +YSS+  LV  + G GV+GFTLD  +GEF+LT P I+IP RG IYS +E  R  W +PL ++++ LQ GKGESGK+YS R +GS VADVHRT
Sbjct:  232 CLSNTLQPGTQLVAAGYCLYSSATTLVFTM-GNGVNGFTLDEQIGEFVLTHPNIRIPNRGKIYSFNESNRNDWDEPLVQYINALQAGKGESGKRYSSRYIGSMVADVHRT 340          
BLAST of mRNA_M-pyrifera_M_contig949.21890.1 vs. uniprot
Match: A0A7S1XRP8_9STRA (Fructose-bisphosphatase n=3 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1XRP8_9STRA)

HSP 1 Score: 134 bits (337), Expect = 2.070e-35
Identity = 64/113 (56.64%), Postives = 83/113 (73.45%), Query Frame = 2
Query:    2 EELCLLRTLQPGRNLVGAGYAMYSSSCELVLAVQGQGVHGFTLDAHVGEFILTRPYIKIPKRGSIYSLDEGRRESWTKPLREHVDKLQRGKGESGKKYSYRLVGSAVADVHRT 340
            ++ CL +TLQPG+ LV +GY MYSSS  LV  + G GV+GFTLD  +GEF+LT P IKIPKRG IYS++E     W  PL+E++D L+ GKGE+G+ YS R +GS V D+HRT
Sbjct:  216 QQECLAKTLQPGKALVASGYCMYSSSTILVFTM-GNGVNGFTLDPTIGEFVLTHPDIKIPKRGKIYSINEANYPHWDAPLKEYIDNLKAGKGETGEAYSARYIGSMVGDIHRT 327          
BLAST of mRNA_M-pyrifera_M_contig949.21890.1 vs. uniprot
Match: M2XAQ5_GALSU (Fructose-bisphosphatase n=1 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2XAQ5_GALSU)

HSP 1 Score: 134 bits (336), Expect = 3.000e-35
Identity = 66/110 (60.00%), Postives = 78/110 (70.91%), Query Frame = 2
Query:   11 CLLRTLQPGRNLVGAGYAMYSSSCELVLAVQGQGVHGFTLDAHVGEFILTRPYIKIPKRGSIYSLDEGRRESWTKPLREHVDKLQRGKGESGKKYSYRLVGSAVADVHRT 340
            CL  TLQPG  L+ AGY MYSSS  +VL++ G G HGFTLD  VGEF+LT PY+KIPKRG IYS +E     W   L+E+V+KL+ G   SGK YS R +GS VADVHRT
Sbjct:  221 CLANTLQPGSRLLAAGYCMYSSSTMMVLSL-GHGTHGFTLDPEVGEFVLTHPYMKIPKRGQIYSFNEANYHQWDPILQEYVEKLKTGNNRSGKCYSLRYIGSLVADVHRT 329          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig949.21890.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KTL6_9PHAE2.080e-5578.76Fructose-bisphosphatase n=2 Tax=Ectocarpus TaxID=2... [more]
X2DAQ5_9ALVE1.810e-3758.93Fructose-bisphosphatase n=1 Tax=Chromera velia Tax... [more]
W7TEP4_9STRA7.900e-3759.09Fructose-bisphosphatase n=1 Tax=Nannochloropsis ga... [more]
A0A813CI76_9DINO1.440e-3657.27Fructose-bisphosphatase (Fragment) n=2 Tax=unclass... [more]
A0A7S2V7Q3_9STRA1.870e-3656.64Fructose-bisphosphatase n=1 Tax=Fibrocapsa japonic... [more]
A0A4D9D3H2_9STRA4.370e-3659.09Uncharacterized protein n=1 Tax=Nannochloropsis sa... [more]
A0A7S1L2B5_ALECA5.030e-3658.18Fructose-bisphosphatase (Fragment) n=1 Tax=Alexand... [more]
A0A836CD45_9STRA1.120e-3559.09Fructose-bisphosphatase n=1 Tax=Tribonema minus Ta... [more]
A0A7S1XRP8_9STRA2.070e-3556.64Fructose-bisphosphatase n=3 Tax=Phaeomonas parva T... [more]
M2XAQ5_GALSU3.000e-3560.00Fructose-bisphosphatase n=1 Tax=Galdieria sulphura... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig949contigM-pyrifera_M_contig949:3217..5383 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons3
Model size343
Cds size342
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig949.21890.1prot_M-pyrifera_M_contig949.21890.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig949 3218..5383 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816862.6335301-CDS-M-pyrifera_M_contig949:3217..33751622816862.6335301-CDS-M-pyrifera_M_contig949:3217..3375Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig949 3218..3375 -
1692278222.459472-CDS-M-pyrifera_M_contig949:3217..33751692278222.459472-CDS-M-pyrifera_M_contig949:3217..3375Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig949 3218..3375 -
1622816862.6469588-CDS-M-pyrifera_M_contig949:4089..42071622816862.6469588-CDS-M-pyrifera_M_contig949:4089..4207Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig949 4090..4207 -
1692278222.4699779-CDS-M-pyrifera_M_contig949:4089..42071692278222.4699779-CDS-M-pyrifera_M_contig949:4089..4207Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig949 4090..4207 -
1622816862.6605382-CDS-M-pyrifera_M_contig949:5317..53831622816862.6605382-CDS-M-pyrifera_M_contig949:5317..5383Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig949 5318..5383 -
1692278222.4778528-CDS-M-pyrifera_M_contig949:5317..53831692278222.4778528-CDS-M-pyrifera_M_contig949:5317..5383Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig949 5318..5383 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig949.21890.1

>prot_M-pyrifera_M_contig949.21890.1 ID=prot_M-pyrifera_M_contig949.21890.1|Name=mRNA_M-pyrifera_M_contig949.21890.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=114bp
GRIVLAEDVAARKEPCRSWICHVQLELRTGAGGAGPRCSRLYSGRPRRRV
YPHSAVHKDPEARQHLFAGRRSEGVVDEAAARARRQAAERQGGVGQEVLL
SARGFRGRGRASHL
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mRNA from alignment at M-pyrifera_M_contig949:3217..5383-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig949.21890.1 ID=mRNA_M-pyrifera_M_contig949.21890.1|Name=mRNA_M-pyrifera_M_contig949.21890.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=2167bp|location=Sequence derived from alignment at M-pyrifera_M_contig949:3217..5383- (Macrocystis pyrifera P11B4 male)
GGAAGAATTGTGCTTGCTGAGGACGTTGCAGCCCGGAAGGAACCTTGTCG GAGCTGGATATGCCATGTGAGATGCAGCGGGTCGGGTGGGTTGGTGGGTG TGTGGGAATGCGCAGCGGGGGGGGGGGGGCGTACTGATTTGTTGCTTGTC TTAGTCATTTGACCATTGAGCAGGGGGGGGGGACAAGGGTACGGTGACTC TGTGATGTGGACTCTGCAATGGGCTAGACGACATGGTTGCGCCCAGCTTT GCTTCGCGAGAGCGCTCCCGTGGGGACTTGTTGGCGTCTCAAAAGTCACA CGACGCTTTTCGGCAGAGGGGGTACTGCGGAGTTTTTACCGTGCATTTTG CTGTGTGATTCGCGACTTCAGGCGGTGTCACTACTATCTGATCATGGTTC TACCGCATGGAAGCTGCTGAACATCGCAGAAGGGTCTTGGCGCCTCGCGA CTGTGTTACCGGGGTTTAGCTCCGGCGAGGTAGTTTCGAGGGCATAAACC GCCTCGAGTAGAGCGCGGCACTGGCCTACCACGTGCATCCTGCAGTGCTC TGCGCGTTTTTTTGTTTAACGTACTCTTGTGTTTTTTTTGTGTGCGTTCA TGTTGATATTGTTCGCCCTGCAGTTCAATGCTCGTGCTTTCCCTAACCCG AGATTACAGAGCAGGCCGCCTCTCCCCCTTCGCGTATTTCTGTTCCTGCC TTACATTTTCTTCGGTGCTCGTTGTGTCCGGCACTGCCAACGCTAGGTGA CTTTTTCGTTGCGCCGGGGTATATATTTTTATAGAGACACATAGCTTTGA ATTGGACGCCGAGAGCGACAATAGCAGTACGCGTCTAGTCTGTTAGTCGC CTCTAAATTGGTTCCGTCTTCACAGCGGTCCGTCCTCTTGACGCGTCCAA GCAGCGCTCCTCTCGCCCCCCCCGCCACCTCGATCCCCGCGCTCATCTCC AAAAGGGTGCGCGTAGCTACATTCATATCGCGAGAGAGGGTTGCGGTGAA GTGTCTGCCCCAACATCGTACAGCCACGGGTAGAGAAGAGTTAAGCTTGG GGAAGAAAGCTTGCTGCCGGATGAGTGCCGTCTTTTTGTTTGCAGAGATC AAGTATGCTTACCACCGTTTTTTTGTGCTTGTCGTTGTTTGGTTGCGCCG CTGCCTCGCGTTTGACTCCGCGACAGGTACAGCTCGAGCTGCGAACTGGT GCTGGCGGTGCAGGGCCAAGGTGTTCACGGCTTTACTCTGGACGCCCACG TCGGCGAGTTTATCCTCACTCGGCCGTACATAAAGATCCCGAAGGTAAAG GGGAACCCTCAGCGGTGGCAGTAGAGGCGGGGAGAGATACCGTTAGTAGT CGCGCCGCACGATGCCTGCCGCTTGTGTGTTTTGTGGCTTTTATCTTTGT TGTGTTTGTGGGTGTCTTGTTCGCCAGTGTGATTTTTTTTTGTGTGCATA CGTGTGACATGAGCGCGCGTGCGGCTCGCGACGCATGGGCAAGAGCTTGA GTTGCTGGGCAGCCTTGGTGGCCCTTTTGGGCGACCCGGAGGCTGCTGCC AGGCAGCACGTCTGGACGTTTTTTTCACGCGTCAATGCCGCCTGTCCTCT TGTGCCCTCGCGGGCACTACTGTGGCGGCAGTGACCTTGTGCGATTCACG TCACCGCCGGTAGAGGAGAGCTCAAAGGTTCGCCAAAAATAGTCATTGTA TGGCACTGCGTTCACCGTCTCGCAGAGCTTGGTTGCTGTTGTAATAATAC TCTCGTACCTTGCAGTTATTGTAGCAATAGGAGGTTGAGACCGACCGACG AAAAATGTTTTATCGTGTAGCAAGCGCCGAAAACTGCGCCGTCGCGGGCG GCGGCGCAATTTGTTACATTGAACCGAATCGATGTTTAGGGTCAAGTCCG CTGTCGGAGCGTTGGCACCCCGCCGGGATTTGTGCTTTTTCGTGCCCCCG TTAACATGCCGCCCTCCTTGCGCGCCGCCGATGCCTGCGTTCTTTTTACG TCCACCAGCGCGGCAGCATCTATTCGCTGGACGAAGGTCGGAGGGAGTCG TGGACGAAGCCGCTGCGCGAGCACGTCGACAAGCTGCAGAGAGGCAAGGG GGAGTCGGGCAAGAAGTACTCCTATCGGCTCGTGGGTTCCGCGGTCGCGG ACGTGCATCGCACCTTG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig949:3217..5383-

>mRNA_M-pyrifera_M_contig949.21890.1 ID=mRNA_M-pyrifera_M_contig949.21890.1|Name=mRNA_M-pyrifera_M_contig949.21890.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=684bp|location=Sequence derived from alignment at M-pyrifera_M_contig949:3217..5383- (Macrocystis pyrifera P11B4 male)
GGAAGAATTGTGCTTGCTGAGGACGTTGCAGCCCGGAAGGAACCTTGTCG
GAGCTGGATATGCCATGGAAGAATTGTGCTTGCTGAGGACGTTGCAGCCC
GGAAGGAACCTTGTCGGAGCTGGATATGCCATGTACAGCTCGAGCTGCGA
ACTGGTGCTGGCGGTGCAGGGCCAAGGTGTTCACGGCTTTACTCTGGACG
CCCACGTCGGCGAGTTTATCCTCACTCGGCCGTACATAAAGATCCCGAAG
GTACAGCTCGAGCTGCGAACTGGTGCTGGCGGTGCAGGGCCAAGGTGTTC
ACGGCTTTACTCTGGACGCCCACGTCGGCGAGTTTATCCTCACTCGGCCG
TACATAAAGATCCCGAAGCGCGGCAGCATCTATTCGCTGGACGAAGGTCG
GAGGGAGTCGTGGACGAAGCCGCTGCGCGAGCACGTCGACAAGCTGCAGA
GAGGCAAGGGGGAGTCGGGCAAGAAGTACTCCTATCGGCTCGTGGGTTCC
GCGGTCGCGGACGTGCATCGCACCTTCGCGGCAGCATCTATTCGCTGGAC
GAAGGTCGGAGGGAGTCGTGGACGAAGCCGCTGCGCGAGCACGTCGACAA
GCTGCAGAGAGGCAAGGGGGAGTCGGGCAAGAAGTACTCCTATCGGCTCG
TGGGTTCCGCGGTCGCGGACGTGCATCGCACCTT
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