mRNA_M-pyrifera_M_contig91885.21277.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig91885.21277.1
Unique NamemRNA_M-pyrifera_M_contig91885.21277.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig91885.21277.1 vs. uniprot
Match: A0A8H7VTT9_9FUNG (Nicastrin n=1 Tax=Mucor circinatus TaxID=2054153 RepID=A0A8H7VTT9_9FUNG)

HSP 1 Score: 82.4 bits (202), Expect = 1.280e-15
Identity = 44/138 (31.88%), Postives = 80/138 (57.97%), Query Frame = 1
Query:   16 WNPRGLELLGRNFDVPMMSVRGGESEA------VWKRAKWNKQRGFSNWPQESVRFSFDMGPSSLRSEECLNKGFCLPLGGQSVWSASR---PLEHQEPAILIATPLDTTSLFHDHSIGAGAELSAIIASLAASRALS 402
            WNP+G  LL   F++P+  +      +      V K  ++N+++G+ N+P ++V F   M  +++ SE CL +G+C  +GG SV+S        + ++  +++   +D+ SLFHD + GAG ++S ++  LAA+ ALS
Sbjct:  145 WNPQGQNLLESEFEIPIFGINPVNKISTQIYNFVMKTVEYNEEKGYDNYPLQAVDFELFMW-AAMNSETCLRRGWCEAIGGLSVYSTPSVDIQADDKKTIVMLTAGMDSRSLFHDLTNGAGHDVSGLVGILAAAEALS 281          
BLAST of mRNA_M-pyrifera_M_contig91885.21277.1 vs. uniprot
Match: NICA_DICPU (Nicastrin n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=NICA_DICPU)

HSP 1 Score: 76.6 bits (187), Expect = 1.330e-13
Identity = 40/117 (34.19%), Postives = 62/117 (52.99%), Query Frame = 1
Query:    1 NGSVVWNPRGLELLGRNFDVPMMSVRGGESEAVWKRAKWNKQRGFSNWPQESVRFSFDMGPSSLRSEECLNKGFCLPLGGQSVWSA-SRPLEHQEPAILIATPLDTTSLFHDHSIGA 348
            N ++ WNP        +F  P+ ++    S A+   +K N+   +  W  E   F       ++ SE CL +GFC P+GGQS+WS+ S  ++ ++  IL+  P DTT+ F D SIGA
Sbjct:  140 NSNLEWNPNADGFTYFSFPFPIFAIDNQTSVAIRNVSKHNRDGQYPAWGAELDSFM----QGAINSETCLRRGFCEPVGGQSIWSSFSSKIDKEKEIILVMLPFDTTAFFRDLSIGA 252          
BLAST of mRNA_M-pyrifera_M_contig91885.21277.1 vs. uniprot
Match: A0A8J4UZP4_9MYCE (Nicastrin n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4UZP4_9MYCE)

HSP 1 Score: 74.3 bits (181), Expect = 8.540e-13
Identity = 39/119 (32.77%), Postives = 62/119 (52.10%), Query Frame = 1
Query:    1 NGSVVWNPRGLELLGRNFDVPMMSVRGGESEAVWKRAKWNKQRGFSNWPQESVRFSFDMGPSSLRSEECLNKGFCLPLGGQSVWSASRPLEHQEPAI-LIATPLDTTSLFHDHSIGAGA 354
            N   +WNP G   L  +  +P+ ++    S A  +    NK+     W  +   F          +E CL +GFC+P+GG+S+W++  P+ +QE  I L+  P+DT + FHD +IGA A
Sbjct:  142 NSEKIWNPLGDSFLYGDVPIPIFALDLNTSIAAREAGGLNKKGTKPTWGAQFDSFMH----GGFDTETCLRRGFCVPIGGKSIWTSFSPVLNQEKEIILVMVPMDTNAYFHDMAIGADA 256          
BLAST of mRNA_M-pyrifera_M_contig91885.21277.1 vs. uniprot
Match: NICA_DICDI (Nicastrin n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=NICA_DICDI)

HSP 1 Score: 67.4 bits (163), Expect = 2.250e-10
Identity = 35/111 (31.53%), Postives = 56/111 (50.45%), Query Frame = 1
Query:   16 WNPRGLELLGRNFDVPMMSVRGGESEAVWKRAKWNKQRGFSNWPQESVRFSFDMGPSSLRSEECLNKGFCLPLGGQSVWSA-SRPLEHQEPAILIATPLDTTSLFHDHSIG 345
            WNP G      NF  PM ++    S  +   +  N+   +  +  E   F       ++ +E CL +GFC P+GGQS+WS+ S  ++  +P IL+  P+D T+ F D + G
Sbjct:  143 WNPNGDGFTYMNFPFPMFALELKTSIIIRNLSTINRDGKYPAYGAELDSFM----QGAINAETCLRRGFCEPVGGQSIWSSFSEVIDQSKPIILVMLPIDATAFFRDLATG 249          
BLAST of mRNA_M-pyrifera_M_contig91885.21277.1 vs. uniprot
Match: L8H287_ACACA (Nicastrin n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8H287_ACACA)

HSP 1 Score: 65.1 bits (157), Expect = 1.450e-9
Identity = 44/139 (31.65%), Postives = 71/139 (51.08%), Query Frame = 1
Query:   16 WNPRGLELLGRNFDVPMMSVRG-GESEAVWKRAKWNKQRGFSNWPQESVRFSFDM---GPSSLRSEECLNKGFCLPLGGQSVW-SASRPLEHQEPAILIATPLDTTSLFHDHSIGAGAELSAIIASLAASRALSNNPNL 417
            WNP G          P+ S+    + + +  +A+ NK+    +WP+ S  F   M   G S++    CL +G+C P+GGQSVW S    ++ +   +L+ + LD+ S+F D ++GA A  S I   L A  AL++   L
Sbjct:  161 WNPCGNAWFMEKLPFPVFSLNSDADGKDLVHKARENKR--LDSWPKWSAEFKAFMWGAGDSNI----CLRRGYCDPVGGQSVWGSFEEGVDAERDIVLVMSQLDSNSIFQDLALGAEATASGITTLLTAIHALADQRKL 293          
BLAST of mRNA_M-pyrifera_M_contig91885.21277.1 vs. uniprot
Match: A0A0L0RWN0_ALLM3 (Nicastrin n=2 Tax=Allomyces macrogynus (strain ATCC 38327) TaxID=578462 RepID=A0A0L0RWN0_ALLM3)

HSP 1 Score: 62.4 bits (150), Expect = 1.250e-8
Identity = 44/141 (31.21%), Postives = 68/141 (48.23%), Query Frame = 1
Query:   16 WNPRGLELLGRNFDVPMMSVRGGESEA------VWKRAKWNKQRGFSNWPQESVRFSFDMGPSSL-RSEECLNKGFCLPLGGQSVWSASRPLEHQEPAILIATPLDTTSLFHDHSIGAGAELSAIIASLAASRALSN-NPN 414
            WNP+G  L   +FD+P+ +     ++       V      N++RG++    + +    D   S   R+  CL +G C P+GGQSV++A    E  +P + +   LD  S FHD +  A   L+   A L A+ AL   NPN
Sbjct:  150 WNPQGTGLFYDSFDLPIFAFANVSTDMASAMKQVQSALTTNRERGYAG--SQVIAAELDAFMSGAHRASNCLRRGMCKPVGGQSVYAAYAN-EFTKPVVFLTATLDGKSFFHDLAPAATKSLTGTAALLTAAVALGQLNPN 287          
BLAST of mRNA_M-pyrifera_M_contig91885.21277.1 vs. uniprot
Match: A0A7S4RNQ6_9STRA (Nicastrin n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4RNQ6_9STRA)

HSP 1 Score: 59.3 bits (142), Expect = 1.540e-7
Identity = 41/128 (32.03%), Postives = 64/128 (50.00%), Query Frame = 1
Query:   64 MMSVRGGESEAVWKRAKWNKQRGFSNWPQESVRFSFDMGPSSLRSEECLN--------KGFCLPLGGQSVWS-ASRPLEHQE---------PAILIATPLDTTSLFHDHSIGAGAELSAIIASLAASR 393
            + SV   +S+ + K+++  K      +P     + + MGP +  ++ CL+        +  CLPLGG SVW  A    E  E         P +++AT +D+TS+FHD S GA    S IIA L A++
Sbjct:  299 LYSVSLEQSKTLVKKSQHGKLDEDDIFPAIVAEYDYYMGPDNQNAKTCLSWVDKDDVWRPKCLPLGGNSVWGVAGSDTEQNEDGGDDENAKPIVMLATSIDSTSMFHDASPGANTAASNIIALLMAAK 426          
BLAST of mRNA_M-pyrifera_M_contig91885.21277.1 vs. uniprot
Match: A0A7S4KX82_9EUKA (Hypothetical protein n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4KX82_9EUKA)

HSP 1 Score: 58.9 bits (141), Expect = 1.920e-7
Identity = 26/68 (38.24%), Postives = 44/68 (64.71%), Query Frame = 1
Query:  184 SSLRSEECLNKGFCLPLGGQSVWSASRPLEHQEPAILIATPLDTTSLFHDHSIGAGAELSAIIASLAA 387
            +++ +  C+ +GFC PL   SV S+  P +  +P IL+ +P+D+TS FHD ++GA AE++ +   L A
Sbjct:    9 AAVDTPTCIRRGFCEPLNANSVISSPLPFQPTKPVILVMSPMDSTSFFHDLAVGASAEVAGMAVLLGA 76          
BLAST of mRNA_M-pyrifera_M_contig91885.21277.1 vs. uniprot
Match: A0A0L0HLD6_SPIPD (Nicastrin n=1 Tax=Spizellomyces punctatus (strain DAOM BR117) TaxID=645134 RepID=A0A0L0HLD6_SPIPD)

HSP 1 Score: 58.9 bits (141), Expect = 2.050e-7
Identity = 35/122 (28.69%), Postives = 59/122 (48.36%), Query Frame = 1
Query:   16 WNPRGLELLGRNFDVPMMSVRGGESEA-----VWKRAKWNKQRGFSNWPQESVRFSFDMGPSSLRSEECLNKGFCLPLGGQSVWS-------ASRPLEHQEPAILIATPLDTTSLFHDHSIG 345
            WNP G  L  ++FD P+  +    S       + +  + N + G+ ++P  +V F   M  +   S  CL +G+C P+GG SVWS       ++  L   +P I+++  +D+ S   D  IG
Sbjct:  166 WNPDGNSLSFQDFDFPIFGISQDGSNLRSITPLKEAVEANARGGYVSYPLYAVEFDAFMWAAG-NSAVCLQRGWCDPIGGLSVWSTFSKVLDSNNTLNDNKPLIVVSAKIDSKSFISDFGIG 286          
BLAST of mRNA_M-pyrifera_M_contig91885.21277.1 vs. uniprot
Match: A0A507F3G5_9FUNG (Nicastrin n=1 Tax=Spizellomyces sp. 'palustris' TaxID=117820 RepID=A0A507F3G5_9FUNG)

HSP 1 Score: 56.6 bits (135), Expect = 1.310e-6
Identity = 36/122 (29.51%), Postives = 60/122 (49.18%), Query Frame = 1
Query:   16 WNPRGLELLGRNFDVPMMSVR--GGESEAVW---KRAKWNKQRGFSNWPQESVRFSFDMGPSSLRSEECLNKGFCLPLGGQSVWS-------ASRPLEHQEPAILIATPLDTTSLFHDHSIG 345
            WNP G  L  ++FD P+  +   G    ++    +  + N + G+ ++P  +V F   M  +   S  CL +G+C P+GG SVWS       ++  L   +P I+I+  +D+ S   D  IG
Sbjct:  166 WNPDGNLLSFQDFDFPIFGISQDGTNLRSITPLKEAVEANARGGYVSYPLYAVEFDAFMWAAG-NSAVCLQRGWCDPVGGLSVWSTFSKVLDSNNSLNDNKPLIVISAKIDSKSFIFDFGIG 286          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig91885.21277.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 10
Match NameE-valueIdentityDescription
A0A8H7VTT9_9FUNG1.280e-1531.88Nicastrin n=1 Tax=Mucor circinatus TaxID=2054153 R... [more]
NICA_DICPU1.330e-1334.19Nicastrin n=1 Tax=Dictyostelium purpureum TaxID=57... [more]
A0A8J4UZP4_9MYCE8.540e-1332.77Nicastrin n=1 Tax=Polysphondylium violaceum TaxID=... [more]
NICA_DICDI2.250e-1031.53Nicastrin n=1 Tax=Dictyostelium discoideum TaxID=4... [more]
L8H287_ACACA1.450e-931.65Nicastrin n=1 Tax=Acanthamoeba castellanii str. Ne... [more]
A0A0L0RWN0_ALLM31.250e-831.21Nicastrin n=2 Tax=Allomyces macrogynus (strain ATC... [more]
A0A7S4RNQ6_9STRA1.540e-732.03Nicastrin n=1 Tax=Ditylum brightwellii TaxID=49249... [more]
A0A7S4KX82_9EUKA1.920e-738.24Hypothetical protein n=1 Tax=Paramoeba aestuarina ... [more]
A0A0L0HLD6_SPIPD2.050e-728.69Nicastrin n=1 Tax=Spizellomyces punctatus (strain ... [more]
A0A507F3G5_9FUNG1.310e-629.51Nicastrin n=1 Tax=Spizellomyces sp. 'palustris' Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig91885contigM-pyrifera_M_contig91885:77..493 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score82.0
Seed ortholog evalue2.4e-13
Seed eggNOG ortholog4565.Traes_6AL_ECEBD0B92.1
KEGG koko:K06171
KEGG Pathwayko04330,ko05010,map04330,map05010
KEGG ModuleM00682
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004175,GO:0005575,GO:0005623,GO:0005886,GO:0005887,GO:0006508,GO:0006807,GO:0008150,GO:0008152,GO:0008233,GO:0010467,GO:0016020,GO:0016021,GO:0016485,GO:0016787,GO:0019538,GO:0031224,GO:0031226,GO:0043170,GO:0044238,GO:0044425,GO:0044459,GO:0044464,GO:0051604,GO:0070011,GO:0071704,GO:0071944,GO:0140096,GO:1901564
EggNOG free text desc.Nicastrin
EggNOG OGs37MBS@33090,3G7N9@35493,3I5HF@38820,3KP3H@4447,KOG2657@1,KOG2657@2759
COG Functional cat.OT
Best tax levelPoales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002
Exons1
Model size417
Cds size417
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816831.5866663-CDS-M-pyrifera_M_contig91885:76..4931622816831.5866663-CDS-M-pyrifera_M_contig91885:76..493Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91885 77..493 +
1692278204.2510815-CDS-M-pyrifera_M_contig91885:76..4931692278204.2510815-CDS-M-pyrifera_M_contig91885:76..493Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91885 77..493 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig91885.21277.1prot_M-pyrifera_M_contig91885.21277.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig91885 77..493 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig91885.21277.1

>prot_M-pyrifera_M_contig91885.21277.1 ID=prot_M-pyrifera_M_contig91885.21277.1|Name=mRNA_M-pyrifera_M_contig91885.21277.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=139bp
NGSVVWNPRGLELLGRNFDVPMMSVRGGESEAVWKRAKWNKQRGFSNWPQ
ESVRFSFDMGPSSLRSEECLNKGFCLPLGGQSVWSASRPLEHQEPAILIA
TPLDTTSLFHDHSIGAGAELSAIIASLAASRALSNNPNL
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mRNA from alignment at M-pyrifera_M_contig91885:77..493+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig91885.21277.1 ID=mRNA_M-pyrifera_M_contig91885.21277.1|Name=mRNA_M-pyrifera_M_contig91885.21277.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=417bp|location=Sequence derived from alignment at M-pyrifera_M_contig91885:77..493+ (Macrocystis pyrifera P11B4 male)
AACGGGAGCGTGGTCTGGAACCCAAGGGGATTGGAGCTTTTGGGCCGAAA TTTTGACGTCCCCATGATGAGCGTGCGTGGGGGGGAATCGGAGGCGGTCT GGAAGAGGGCAAAGTGGAACAAGCAGAGAGGATTCTCCAATTGGCCGCAA GAAAGCGTCCGGTTCAGTTTTGACATGGGCCCTTCTTCATTGCGATCCGA AGAATGCCTGAACAAAGGGTTTTGCCTCCCACTTGGGGGACAGAGCGTGT GGAGCGCTTCCCGACCGTTGGAACACCAAGAACCAGCCATTCTCATCGCC ACCCCGCTTGACACAACCTCTCTCTTCCACGACCACTCGATAGGGGCAGG AGCTGAACTAAGTGCTATTATTGCGTCGCTTGCAGCCTCAAGAGCCCTCT CCAACAACCCCAACCTA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig91885:77..493+

>mRNA_M-pyrifera_M_contig91885.21277.1 ID=mRNA_M-pyrifera_M_contig91885.21277.1|Name=mRNA_M-pyrifera_M_contig91885.21277.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=834bp|location=Sequence derived from alignment at M-pyrifera_M_contig91885:77..493+ (Macrocystis pyrifera P11B4 male)
AACGGGAGCGTGGTCTGGAACCCAAGGGGATTGGAGCTTTTGGGCCGAAA
TTTTGACGTCCCCATGATGAGCGTGCGTGGGGGGGAATCGGAGGCGGTCT
GGAAGAGGGCAAAGTGGAACAAGCAGAGAGGATTCTCCAATTGGCCGCAA
GAAAGCGTCCGGTTCAGTTTTGACATGGGCCCTTCTTCATTGCGATCCGA
AGAATGCCTGAACAAAGGGTTTTGCCTCCCACTTGGGGGACAGAGCGTGT
GGAGCGCTTCCCGACCGTTGGAACACCAAGAACCAGCCATTCTCATCGCC
ACCCCGCTTGACACAACCTCTCTCTTCCACGACCACTCGATAGGGGCAGG
AGCTGAACTAAGTGCTATTATTGCGTCGCTTGCAGCCTCAAGAGCCCTCT
CCAACAACCCCAACCTAAACGGGAGCGTGGTCTGGAACCCAAGGGGATTG
GAGCTTTTGGGCCGAAATTTTGACGTCCCCATGATGAGCGTGCGTGGGGG
GGAATCGGAGGCGGTCTGGAAGAGGGCAAAGTGGAACAAGCAGAGAGGAT
TCTCCAATTGGCCGCAAGAAAGCGTCCGGTTCAGTTTTGACATGGGCCCT
TCTTCATTGCGATCCGAAGAATGCCTGAACAAAGGGTTTTGCCTCCCACT
TGGGGGACAGAGCGTGTGGAGCGCTTCCCGACCGTTGGAACACCAAGAAC
CAGCCATTCTCATCGCCACCCCGCTTGACACAACCTCTCTCTTCCACGAC
CACTCGATAGGGGCAGGAGCTGAACTAAGTGCTATTATTGCGTCGCTTGC
AGCCTCAAGAGCCCTCTCCAACAACCCCAACCTA
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