mRNA_M-pyrifera_M_contig91874.21271.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig91874.21271.1
Unique NamemRNA_M-pyrifera_M_contig91874.21271.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig91874.21271.1 vs. uniprot
Match: A0A517WAF5_9PLAN (Autoinducer 2 sensor kinase/phosphatase LuxQ n=8 Tax=Gimesia TaxID=1649453 RepID=A0A517WAF5_9PLAN)

HSP 1 Score: 313 bits (801), Expect = 1.330e-95
Identity = 160/174 (91.95%), Postives = 166/174 (95.40%), Query Frame = 1
Query:    1 ISATSQPGEGSTFTCAIQLPPGDHVKLIQPQLKTDTPEPVSMLDTYQLNCRILIVDDQRDVRHLAKLLLQRAGAETEFAEDGLQAVELIKQQLQRESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSRLTQHTT 522
            IS TSQPGEGSTFTCAI+LPP +HVKLIQPQL T+ PEPVS+LDT QLNCRILIVDDQRDVRHLA+LLLQRAGAETEFAEDGLQAVELI+QQLQ ESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSRLTQ  T
Sbjct: 1601 ISVTSQPGEGSTFTCAIKLPPCEHVKLIQPQLNTEPPEPVSILDTCQLNCRILIVDDQRDVRHLARLLLQRAGAETEFAEDGLQAVELIEQQLQTESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSRLTQQPT 1774          
BLAST of mRNA_M-pyrifera_M_contig91874.21271.1 vs. uniprot
Match: A0A5A8AU59_9PLAN (PAS domain-containing protein n=2 Tax=Gimesia chilikensis TaxID=2605989 RepID=A0A5A8AU59_9PLAN)

HSP 1 Score: 282 bits (721), Expect = 7.970e-85
Identity = 144/170 (84.71%), Postives = 151/170 (88.82%), Query Frame = 1
Query:    1 ISATSQPGEGSTFTCAIQLPPGDHVKLIQPQLKTDTPEPVSMLDTYQLNCRILIVDDQRDVRHLAKLLLQRAGAETEFAEDGLQAVELIKQQLQRESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSRLT 510
            IS TSQPGEGSTFTC IQLPP DHVKLIQP+L    PEPVS+LD  +LNCRILIVDDQRDVR LAKLLLQR  AETEFAEDGLQAVELI+QQL  ESTADLILLDMQMPRMDGYQT ARLREIGYRKPI+ALTADAMQGDMKRCLELGCDDYLSKPIDA EL ++VS  T
Sbjct: 1604 ISVTSQPGEGSTFTCVIQLPPDDHVKLIQPELNLQAPEPVSILDACKLNCRILIVDDQRDVRQLAKLLLQRVEAETEFAEDGLQAVELIEQQLHTESTADLILLDMQMPRMDGYQTVARLREIGYRKPIIALTADAMQGDMKRCLELGCDDYLSKPIDASELLKVVSTYT 1773          
BLAST of mRNA_M-pyrifera_M_contig91874.21271.1 vs. uniprot
Match: A0A2D5H1J1_9PLAN (Response regulatory domain-containing protein n=2 Tax=Planctomycetaceae TaxID=126 RepID=A0A2D5H1J1_9PLAN)

HSP 1 Score: 230 bits (586), Expect = 1.790e-74
Identity = 116/168 (69.05%), Postives = 135/168 (80.36%), Query Frame = 1
Query:    1 ISATSQPGEGSTFTCAIQLPPGDHVKLIQPQLKTDTPEPVSMLDTYQLNCRILIVDDQRDVRHLAKLLLQRAGAETEFAEDGLQAVELIKQQLQRESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSR 504
            IS +S+PGEGSTF+C I LP  ++VK+  P L+    + ++ +  YQL CR+LIVDDQRDVRHL  LLLQ AG ETEFAED +QAVELI+ QLQ   T DLILLDMQMPRMDGYQ AARLREIG+R+ I+ LTADAMQGDM RCLELGCDDYLSKPID+ EL EM+SR
Sbjct:    6 ISVSSKPGEGSTFSCTINLPVRENVKITSPNLEVVPIKAITTVREYQLQCRVLIVDDQRDVRHLTHLLLQGAGIETEFAEDRIQAVELIENQLQNGLTVDLILLDMQMPRMDGYQAAARLREIGFRRAIIVLTADAMQGDMNRCLELGCDDYLSKPIDSSELLEMISR 173          
BLAST of mRNA_M-pyrifera_M_contig91874.21271.1 vs. uniprot
Match: A0A517X997_9PLAN (Autoinducer 2 sensor kinase/phosphatase LuxQ n=4 Tax=Gimesia maris TaxID=122 RepID=A0A517X997_9PLAN)

HSP 1 Score: 241 bits (615), Expect = 1.450e-70
Identity = 121/170 (71.18%), Postives = 140/170 (82.35%), Query Frame = 1
Query:    1 ISATSQPGEGSTFTCAIQLPPGDHVKLIQPQLKTDTPEPVSMLDTYQLNCRILIVDDQRDVRHLAKLLLQRAGAETEFAEDGLQAVELIKQQLQRESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSRLT 510
            IS +S+PGEGSTF+C I LP  ++VK+  P L+    + ++ +  YQL CR+LIVDDQRDVRHL  LLLQRAG ETEFAEDG+QAVELI+ QLQ   T DLILLDMQMPRMDGYQTAARLREIG+R+ I+ALTADAMQGDM RCLELGCDDYLSKPID+ EL EM+SR T
Sbjct: 1605 ISVSSKPGEGSTFSCTINLPVRENVKITSPNLEVVPIKAITTVREYQLQCRVLIVDDQRDVRHLTHLLLQRAGIETEFAEDGIQAVELIENQLQNGLTVDLILLDMQMPRMDGYQTAARLREIGFRRAIIALTADAMQGDMNRCLELGCDDYLSKPIDSNELLEMISRYT 1774          
BLAST of mRNA_M-pyrifera_M_contig91874.21271.1 vs. uniprot
Match: A0A517VHR1_9PLAN (Protein-glutamate O-methyltransferase n=4 Tax=Planctomycetaceae TaxID=126 RepID=A0A517VHR1_9PLAN)

HSP 1 Score: 229 bits (584), Expect = 2.150e-66
Identity = 118/170 (69.41%), Postives = 136/170 (80.00%), Query Frame = 1
Query:    1 ISATSQPGEGSTFTCAIQLPPGDHVKLIQPQLKTDTPEPVSMLDTYQLNCRILIVDDQRDVRHLAKLLLQRAGAETEFAEDGLQAVELIKQQLQRESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSRLT 510
            IS +S+PGEGSTF+C I LP  ++VKL    L+    +  + L  YQL CR+LIVDDQRDVRHL  LLLQRAG ETEFA DG+QAVELI+ QLQ   T DLILLDMQMPRMDGYQTAARLREIG+ + I+ALTADAMQGDM RCLELGCDDYLSKPI++ EL +M+SR T
Sbjct: 1605 ISVSSKPGEGSTFSCTINLPVRENVKLTNLNLEAVPLKTETALREYQLQCRVLIVDDQRDVRHLTNLLLQRAGVETEFAVDGIQAVELIENQLQNGLTVDLILLDMQMPRMDGYQTAARLREIGFSRAIIALTADAMQGDMNRCLELGCDDYLSKPINSSELLKMISRYT 1774          
BLAST of mRNA_M-pyrifera_M_contig91874.21271.1 vs. uniprot
Match: A0A355G7X7_9PLAN (ATPase (Fragment) n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A355G7X7_9PLAN)

HSP 1 Score: 182 bits (461), Expect = 1.470e-54
Identity = 93/173 (53.76%), Postives = 124/173 (71.68%), Query Frame = 1
Query:    1 ISATSQPGEGSTFTCAIQLPPGDHVKLIQPQLK-TDTPEPVSMLDTYQ-LNCRILIVDDQRDVRHLAKLLLQRAGAETEFAEDGLQAVELIKQQLQRESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSRLTQ 513
            I  TSQPG G+TFTC I     D V ++ P+ K   TP+    LDT   L+C +L+VDD+RD+R+L K  L ++GAE E   DGL+A+E ++Q    +++ D+ILLDMQMPR+DGYQTA RLR +G+ +PI+ALTADAM GDM RCL  GCD +LSKPI+ +EL E+V+R TQ
Sbjct:   81 IEVTSQPGIGTTFTCKISAGQVDSVPMVHPEKKHKSTPDTSKSLDTQPALSCNVLVVDDRRDIRYLVKQFLTKSGAEVESVNDGLEAIERVEQ---GQNSFDMILLDMQMPRLDGYQTAERLRSLGFNRPIIALTADAMHGDMNRCLASGCDAFLSKPINTKELIEIVARYTQ 250          
BLAST of mRNA_M-pyrifera_M_contig91874.21271.1 vs. uniprot
Match: A0A517MX98_9BACT (Signal transduction histidine-protein kinase BarA n=1 Tax=Adhaeretor mobilis TaxID=1930276 RepID=A0A517MX98_9BACT)

HSP 1 Score: 187 bits (474), Expect = 1.350e-51
Identity = 95/173 (54.91%), Postives = 122/173 (70.52%), Query Frame = 1
Query:    1 ISATSQPGEGSTFTCAIQLPPGDHVKLIQPQLKTDTPEPVSMLDTYQLNCRILIVDDQRDVRHLAKLLLQRAGAETEFAEDGLQAVELIKQQLQRESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSRLTQHT 519
            I+  S PG+GSTF+C I       V+LI+P L T     VS    ++LNCR+L+VDD+RDVR L   +L  AG E   AEDG++A++++K+   R  T DLILLDMQMPR+DGYQTAARLRE+ + +PI+ALTADAM GDM  CL  GC+ YLSKPIDA++L EMV+  T  T
Sbjct: 1749 ITVDSVPGQGSTFSCVISAGDVSDVQLIKPALDTQEQPSVSRPTEHRLNCRVLVVDDRRDVRFLTMRILSSAGVEVSLAEDGIEALQMVKELAARGETWDLILLDMQMPRLDGYQTAARLREMQFTQPIIALTADAMHGDMNHCLRSGCNAYLSKPIDAQKLIEMVAEYTGQT 1921          
BLAST of mRNA_M-pyrifera_M_contig91874.21271.1 vs. uniprot
Match: A0A7Y2AEL3_9BACT (Response regulator n=1 Tax=Pirellulaceae bacterium TaxID=2699754 RepID=A0A7Y2AEL3_9BACT)

HSP 1 Score: 175 bits (444), Expect = 2.850e-50
Identity = 92/173 (53.18%), Postives = 117/173 (67.63%), Query Frame = 1
Query:    1 ISATSQPGEGSTFTCAIQLPPGDHVKLIQPQLKTDTPE---PVSMLDTYQLNCRILIVDDQRDVRHLAKLLLQRAGAETEFAEDGLQAVELIKQQLQRESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSRLT 510
            IS  S+ G+GS F   +       V+ I P  +TDTP    P +     QL+C++L+VDD+RDVR LA  +L+  GA  EF EDGL+A E I+  +      DLILLDMQMPR+DGYQ AARLR++G++ PIVALTADAM GDM RC++ GCDDYLSKPID  EL + V+R T
Sbjct:  225 ISFVSRVGQGSQFKFVVAAGDITDVERIDPSERTDTPSVDPPATQESGQQLDCKVLVVDDRRDVRFLAGHILRNHGATVEFCEDGLEACEFIESAMDSGQLPDLILLDMQMPRLDGYQAAARLRKLGFQNPIVALTADAMHGDMTRCIQSGCDDYLSKPIDTSELLQTVARFT 397          
BLAST of mRNA_M-pyrifera_M_contig91874.21271.1 vs. uniprot
Match: A0A2E2BWH3_9PLAN (Uncharacterized protein n=5 Tax=Planctomycetaceae TaxID=126 RepID=A0A2E2BWH3_9PLAN)

HSP 1 Score: 182 bits (461), Expect = 3.890e-50
Identity = 93/173 (53.76%), Postives = 124/173 (71.68%), Query Frame = 1
Query:    1 ISATSQPGEGSTFTCAIQLPPGDHVKLIQPQLK-TDTPEPVSMLDTYQ-LNCRILIVDDQRDVRHLAKLLLQRAGAETEFAEDGLQAVELIKQQLQRESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSRLTQ 513
            I  TSQPG G+TFTC I     D V ++ P+ K   TP+    LDT   L+C +L+VDD+RD+R+L K  L ++GAE E   DGL+A+E ++Q    +++ D+ILLDMQMPR+DGYQTA RLR +G+ +PI+ALTADAM GDM RCL  GCD +LSKPI+ +EL E+V+R TQ
Sbjct:  675 IEVTSQPGIGTTFTCKISAGQVDSVPMVHPEKKHKSTPDTSKSLDTQPALSCNVLVVDDRRDIRYLVKQFLTKSGAEVESVNDGLEAIERVEQ---GQNSFDMILLDMQMPRLDGYQTAERLRSLGFNRPIIALTADAMHGDMNRCLASGCDAFLSKPINTKELIEIVARYTQ 844          
BLAST of mRNA_M-pyrifera_M_contig91874.21271.1 vs. uniprot
Match: A0A517M7A9_9BACT (Protein-glutamate O-methyltransferase n=2 Tax=Rosistilla ulvae TaxID=1930277 RepID=A0A517M7A9_9BACT)

HSP 1 Score: 182 bits (461), Expect = 7.570e-50
Identity = 93/170 (54.71%), Postives = 124/170 (72.94%), Query Frame = 1
Query:    1 ISATSQPGEGSTFTCAIQLPPGDHVKLIQPQLKTDTPEPVSMLDTYQLNCRILIVDDQRDVRHLAKLLLQRAGAETEFAEDGLQAVELIKQQLQRESTADLILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCDDYLSKPIDARELFEMVSRLT 510
            IS  S   EGSTFT  I     D V LIQP+L T+  +  ++ +   LNCRIL+VDD+RD+R L++ LL +AGAE + AEDG  A+ +++++L R+   DL+LLDMQMPR+DGYQTA +LR++G+  PI+ALTADAMQGDM RC+E GC+DYLSKPI+ + L  MV R T
Sbjct: 1911 ISVCSNLHEGSTFTVTIATGDIDKVPLIQPRLTTELTQEETVGEKITLNCRILVVDDRRDIRFLSRRLLTKAGAEVDEAEDGQVALTMVREKLARDEWYDLVLLDMQMPRLDGYQTAQQLRKLGFAGPIIALTADAMQGDMTRCIECGCNDYLSKPINVQRLTSMVHRFT 2080          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig91874.21271.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A517WAF5_9PLAN1.330e-9591.95Autoinducer 2 sensor kinase/phosphatase LuxQ n=8 T... [more]
A0A5A8AU59_9PLAN7.970e-8584.71PAS domain-containing protein n=2 Tax=Gimesia chil... [more]
A0A2D5H1J1_9PLAN1.790e-7469.05Response regulatory domain-containing protein n=2 ... [more]
A0A517X997_9PLAN1.450e-7071.18Autoinducer 2 sensor kinase/phosphatase LuxQ n=4 T... [more]
A0A517VHR1_9PLAN2.150e-6669.41Protein-glutamate O-methyltransferase n=4 Tax=Plan... [more]
A0A355G7X7_9PLAN1.470e-5453.76ATPase (Fragment) n=1 Tax=Planctomycetaceae bacter... [more]
A0A517MX98_9BACT1.350e-5154.91Signal transduction histidine-protein kinase BarA ... [more]
A0A7Y2AEL3_9BACT2.850e-5053.18Response regulator n=1 Tax=Pirellulaceae bacterium... [more]
A0A2E2BWH3_9PLAN3.890e-5053.76Uncharacterized protein n=5 Tax=Planctomycetaceae ... [more]
A0A517M7A9_9BACT7.570e-5054.71Protein-glutamate O-methyltransferase n=2 Tax=Rosi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig91874contigM-pyrifera_M_contig91874:3..527 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score243.8
Seed ortholog evalue5.9e-62
Seed eggNOG ortholog344747.PM8797T_02639
Preferred namecheR4
KEGG koko:K13924
KEGG Pathwayko02020,ko02030,map02020,map02030
KEGG ModuleM00506
Hectar predicted targeting categoryother localisation
EggNOG free text desc.PhoQ Sensor
EggNOG OGsCOG0642@1,COG1352@1,COG1352@2,COG2205@2
EC2.1.1.80,3.1.1.61
COG Functional cat.T
Best tax levelBacteria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko02022,ko02035
Exons1
Model size525
Cds size525
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816831.3103-CDS-M-pyrifera_M_contig91874:2..5271622816831.3103-CDS-M-pyrifera_M_contig91874:2..527Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91874 3..527 +
1692278204.1004646-CDS-M-pyrifera_M_contig91874:2..5271692278204.1004646-CDS-M-pyrifera_M_contig91874:2..527Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91874 3..527 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig91874.21271.1prot_M-pyrifera_M_contig91874.21271.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig91874 3..527 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig91874.21271.1

>prot_M-pyrifera_M_contig91874.21271.1 ID=prot_M-pyrifera_M_contig91874.21271.1|Name=mRNA_M-pyrifera_M_contig91874.21271.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=175bp
ISATSQPGEGSTFTCAIQLPPGDHVKLIQPQLKTDTPEPVSMLDTYQLNC
RILIVDDQRDVRHLAKLLLQRAGAETEFAEDGLQAVELIKQQLQRESTAD
LILLDMQMPRMDGYQTAARLREIGYRKPIVALTADAMQGDMKRCLELGCD
DYLSKPIDARELFEMVSRLTQHTT*
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mRNA from alignment at M-pyrifera_M_contig91874:3..527+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig91874.21271.1 ID=mRNA_M-pyrifera_M_contig91874.21271.1|Name=mRNA_M-pyrifera_M_contig91874.21271.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=525bp|location=Sequence derived from alignment at M-pyrifera_M_contig91874:3..527+ (Macrocystis pyrifera P11B4 male)
ATTTCCGCAACGAGCCAACCGGGAGAGGGGAGCACGTTCACCTGCGCCAT CCAGTTACCTCCCGGCGATCATGTGAAACTGATTCAACCACAGCTGAAAA CGGATACACCGGAGCCGGTAAGTATGCTGGATACCTACCAGTTAAACTGC CGGATCCTGATCGTCGATGATCAACGCGATGTGCGGCATCTGGCAAAACT GCTGCTGCAACGGGCCGGCGCTGAGACCGAATTTGCGGAAGATGGTCTGC AGGCCGTGGAACTGATCAAGCAGCAGTTGCAGCGTGAATCTACTGCAGAT TTGATACTGCTGGATATGCAGATGCCCCGGATGGACGGATATCAGACAGC GGCCCGCCTGCGTGAAATCGGCTATCGCAAGCCAATTGTCGCACTGACTG CCGATGCGATGCAGGGGGACATGAAACGCTGTCTGGAACTGGGCTGCGAT GACTACTTGAGCAAGCCCATCGATGCCAGAGAGCTGTTTGAAATGGTCTC CCGATTGACGCAGCACACAACCTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig91874:3..527+

>mRNA_M-pyrifera_M_contig91874.21271.1 ID=mRNA_M-pyrifera_M_contig91874.21271.1|Name=mRNA_M-pyrifera_M_contig91874.21271.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1050bp|location=Sequence derived from alignment at M-pyrifera_M_contig91874:3..527+ (Macrocystis pyrifera P11B4 male)
ATTTCCGCAACGAGCCAACCGGGAGAGGGGAGCACGTTCACCTGCGCCAT
CCAGTTACCTCCCGGCGATCATGTGAAACTGATTCAACCACAGCTGAAAA
CGGATACACCGGAGCCGGTAAGTATGCTGGATACCTACCAGTTAAACTGC
CGGATCCTGATCGTCGATGATCAACGCGATGTGCGGCATCTGGCAAAACT
GCTGCTGCAACGGGCCGGCGCTGAGACCGAATTTGCGGAAGATGGTCTGC
AGGCCGTGGAACTGATCAAGCAGCAGTTGCAGCGTGAATCTACTGCAGAT
TTGATACTGCTGGATATGCAGATGCCCCGGATGGACGGATATCAGACAGC
GGCCCGCCTGCGTGAAATCGGCTATCGCAAGCCAATTGTCGCACTGACTG
CCGATGCGATGCAGGGGGACATGAAACGCTGTCTGGAACTGGGCTGCGAT
GACTACTTGAGCAAGCCCATCGATGCCAGAGAGCTGTTTGAAATGGTCTC
CCGATTGACGCAGCACACAACCTGAATTTCCGCAACGAGCCAACCGGGAG
AGGGGAGCACGTTCACCTGCGCCATCCAGTTACCTCCCGGCGATCATGTG
AAACTGATTCAACCACAGCTGAAAACGGATACACCGGAGCCGGTAAGTAT
GCTGGATACCTACCAGTTAAACTGCCGGATCCTGATCGTCGATGATCAAC
GCGATGTGCGGCATCTGGCAAAACTGCTGCTGCAACGGGCCGGCGCTGAG
ACCGAATTTGCGGAAGATGGTCTGCAGGCCGTGGAACTGATCAAGCAGCA
GTTGCAGCGTGAATCTACTGCAGATTTGATACTGCTGGATATGCAGATGC
CCCGGATGGACGGATATCAGACAGCGGCCCGCCTGCGTGAAATCGGCTAT
CGCAAGCCAATTGTCGCACTGACTGCCGATGCGATGCAGGGGGACATGAA
ACGCTGTCTGGAACTGGGCTGCGATGACTACTTGAGCAAGCCCATCGATG
CCAGAGAGCTGTTTGAAATGGTCTCCCGATTGACGCAGCACACAACCTGA
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