mRNA_M-pyrifera_M_contig91760.21250.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig91760.21250.1
Unique NamemRNA_M-pyrifera_M_contig91760.21250.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig91760.21250.1 vs. uniprot
Match: A0A7Y2ICZ1_9ACTN (AAA family ATPase n=1 Tax=Acidimicrobiales bacterium TaxID=2201156 RepID=A0A7Y2ICZ1_9ACTN)

HSP 1 Score: 143 bits (360), Expect = 6.920e-36
Identity = 86/199 (43.22%), Postives = 115/199 (57.79%), Query Frame = 1
Query:   10 VRIEPQVFDVLAYLVEHRERVVSKEELLDEIWGDRFVSESALTTRIKMARRAVGDDGRAQRLIRNSHGRGYQFIGEVVSSAAREPNERPIAESSQSEPDVRVPPAASPTKQAADFALLVDEEFAFVGREHLLDEASVHVERAVSGGSGALLIGGEPGIGKTRLAAEIARSSQRTHGLMALAGRCDRHLASSLQPWLEAL 606
            + +E QVFDVL  LV + +RVV K ELLD +WGD+FVSESALT+RIK AR+A+GDDGRAQR I+  HG GY+F+  + +           A +S +       P A P        + +D+EF FVGR    D     ++    G S   LIGGE G+GK+R A EIA+ +    G +  AGRCDR +   LQ W + +
Sbjct:   35 IHLERQVFDVLEVLVNNADRVVGKAELLDTVWGDQFVSESALTSRIKSARQALGDDGRAQRCIKTVHGVGYRFVAPLTADGT-------AASTSPAIESAGFTPTAQPN---VALTIGIDDEFPFVGRAEETDRLWTLLD-GKPGRSAVGLIGGEAGVGKSRFAIEIAQRAAVEFGAVVGAGRCDREVVGHLQAWRDVV 222          
BLAST of mRNA_M-pyrifera_M_contig91760.21250.1 vs. uniprot
Match: UPI001B359D07 (AAA family ATPase n=2 Tax=Micromonospora sp. D93 TaxID=2824886 RepID=UPI001B359D07)

HSP 1 Score: 142 bits (358), Expect = 1.300e-35
Identity = 89/203 (43.84%), Postives = 115/203 (56.65%), Query Frame = 1
Query:    1 GVAVRIEPQVFDVLAYLVEHRERVVSKEELLDEIWGDRFVSESALTTRIKMARRAVGDDGRAQRLIRNSHGRGYQFIGEVVSSAAREPNERPIAESSQSEPDVRVPPAASPTKQAADFALLVDEEFAFVGREH-LLDEASVHVERAVSGGSGALLIGGEPGIGKTRLAAEIARSSQRTHGLMALAGRCDRHLASSLQPWLEAL 606
            G  + +EP+ FDVL YL+ HR+RVVSKEELLD +WGDRFVSE+++TT ++  R A+GD GR  RLIR ++ RGYQF+G V+ + A             SE DVR+P             L V     F GREH  +  A +  E   SG    +L+ GE GIGKT L A  A ++ R    + L GRCD  L+   QPW E L
Sbjct:   24 GRRIPLEPRAFDVLHYLISHRDRVVSKEELLDSVWGDRFVSEASVTTVLRTIRVAIGDAGREHRLIRTAYRRGYQFVGTVMQTTA--------GAGPGSEHDVRLPDR-----------LAVSSGLGFAGREHERVVLADLWKEVLASGQRRVVLVSGEAGIGKTTLCAVFAAAAHRDS--VVLYGRCDEELSIPYQPWREVL 205          
BLAST of mRNA_M-pyrifera_M_contig91760.21250.1 vs. uniprot
Match: A0A848UJF2_9ACTN (AAA family ATPase (Fragment) n=1 Tax=Ilumatobacter sp. TaxID=1967498 RepID=A0A848UJF2_9ACTN)

HSP 1 Score: 138 bits (347), Expect = 1.380e-35
Identity = 91/199 (45.73%), Postives = 111/199 (55.78%), Query Frame = 1
Query:   19 EPQVFDVLAYLVEHRERVVSKEELLDEIWGDRFVSESALTTRIKMARRAVGDDGRAQRLIRNSHGRGYQFIGEVVSSAAREPNERPIAESSQSEPDVRVPPAASPTKQAADFA---LLVDEEFAFVGREHLLDEASVHVERAVSGGSGALLIGGEPGIGKTRLAAEIARSSQRTHGLMALAGRCDRHLASSLQPWLEAL 606
            EPQVFDVL YLVE   R+V+KEELLD +WGDRFVSESALTTRIK ARR + DDGR+Q  IR   GRG++F+  V   AA EP                       T  +AD     L  +    FVGR   +  A   +ER     +   ++G EPGIGKTRLAA IA+ +    G+  L GRC   LA   QP +EA+
Sbjct:   28 EPQVFDVLRYLVEQHGRLVTKEELLDNVWGDRFVSESALTTRIKQARRLIDDDGRSQSRIRTIRGRGFRFLTPVAERAANEPT----------------------TSASADVLPRELAGERTKPFVGRAAEVRRALAVLERPSRTATWIQILG-EPGIGKTRLAARIAQLATE-RGIAVLFGRCSEDLAVPYQPVIEAI 202          
BLAST of mRNA_M-pyrifera_M_contig91760.21250.1 vs. uniprot
Match: A0A4R4MSF9_9ACTN (OmpR/PhoB-type domain-containing protein n=2 Tax=Actinomadura sp. 7K534 TaxID=2530366 RepID=A0A4R4MSF9_9ACTN)

HSP 1 Score: 140 bits (352), Expect = 8.270e-35
Identity = 89/203 (43.84%), Postives = 112/203 (55.17%), Query Frame = 1
Query:    1 GVAVRIEPQVFDVLAYLVEHRERVVSKEELLDEIWGDRFVSESALTTRIKMARRAVGDDGRAQRLIRNSHGRGYQFIGEVVSSAAREPNERPIAESSQSEPDVRVPPAASPTKQAADFALLVDEEFAFVGREHLLDE-ASVHVERAVSGGSGALLIGGEPGIGKTRLAAEIARSSQRTHGLMALAGRCDRHLASSLQPWLEAL 606
            G  V +EP+  DVL +L+ HR+RVVSKEELLD +WGDRFVS++A+TT ++ AR A+GD GR QRLIR    RGYQF+G VV +AA    E P               A+ P +      L V     F GREH     A    E A SG    + + GE GIGKT L +  A ++ R    + L GRCD  L+   QPW E L
Sbjct:   33 GKRVPVEPRALDVLHHLISHRDRVVSKEELLDAVWGDRFVSDAAVTTALRTARAAIGDTGRDQRLIRTVFRRGYQFVGTVVQTAAGRGPEAPRD-------------ASLPAR------LAVSSGLGFAGREHERTVLAGAWKEVAASGHRRVVFVSGEAGIGKTTLCSVFAAATHRE--AVVLYGRCDEELSIPYQPWREVL 214          
BLAST of mRNA_M-pyrifera_M_contig91760.21250.1 vs. uniprot
Match: A0A1C5JKI6_9ACTN (Transcriptional regulatory protein, C terminal n=1 Tax=Micromonospora halophytica TaxID=47864 RepID=A0A1C5JKI6_9ACTN)

HSP 1 Score: 139 bits (350), Expect = 1.530e-34
Identity = 91/203 (44.83%), Postives = 117/203 (57.64%), Query Frame = 1
Query:    1 GVAVRIEPQVFDVLAYLVEHRERVVSKEELLDEIWGDRFVSESALTTRIKMARRAVGDDGRAQRLIRNSHGRGYQFIGEVVSSAAREPNERPIAESSQSEPDVRVPPAASPTKQAADFALLVDEEFAFVGREHLLDE-ASVHVERAVSGGSGALLIGGEPGIGKTRLAAEIARSSQRTHGLMALAGRCDRHLASSLQPWLEAL 606
            G  + +EP+ FDVL +L+ HR+RVVSKEELLD +WGDRFVS++A+TT ++  R A+GD GR QRLIR ++ RGYQF+G V+ +AAR    RP +          VP  A          L V    AF GREH     A V  E   SG    +L+ GE GIGKT L +  A ++ R    + L GRCD  L+   QPW E L
Sbjct:   24 GKRIPLEPRAFDVLHHLISHRDRVVSKEELLDSVWGDRFVSDAAVTTVLRTIRVAIGDAGREQRLIRTAYRRGYQFVGAVMQAAARG---RPGSGHG-------VPLPAR---------LAVSSGLAFAGREHERAVLADVWKEVTASGQRRVVLVSGEAGIGKTTLCSVFAAAAHRE--AVMLYGRCDEELSVPYQPWRELL 205          
BLAST of mRNA_M-pyrifera_M_contig91760.21250.1 vs. uniprot
Match: A0A6N7FR29_9ACTN (Alpha/beta fold hydrolase n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A6N7FR29_9ACTN)

HSP 1 Score: 135 bits (340), Expect = 1.640e-34
Identity = 65/91 (71.43%), Postives = 75/91 (82.42%), Query Frame = 1
Query:    1 GVAVRIEPQVFDVLAYLVEHRERVVSKEELLDEIWGDRFVSESALTTRIKMARRAVGDDGRAQRLIRNSHGRGYQFIGEVVSSAAREPNER 273
            GVAV +EPQVFDVLAYL+EHR+RVV K ELLD +WGDRFV ESALT+R+K ARRAVGDDG AQR+IR +HGRGYQF+ +V   A  EP  R
Sbjct:   21 GVAVHLEPQVFDVLAYLIEHRDRVVPKTELLDRVWGDRFVGESALTSRLKAARRAVGDDGAAQRVIRTAHGRGYQFVADVHEQAPEEPAHR 111          
BLAST of mRNA_M-pyrifera_M_contig91760.21250.1 vs. uniprot
Match: A0A239F314_9ACTN (Transcriptional regulatory protein, C terminal n=3 Tax=Actinomadura TaxID=1988 RepID=A0A239F314_9ACTN)

HSP 1 Score: 137 bits (344), Expect = 9.780e-34
Identity = 89/205 (43.41%), Postives = 116/205 (56.59%), Query Frame = 1
Query:    1 GVAVRIEPQVFDVLAYLVEHRERVVSKEELLDEIWGDRFVSESALTTRIKMARRAVGDDGRAQRLIRNSHGRGYQFIGEVVSSAAREPNERPIAESSQSEPDVRVPPAASPTKQAADFALLVDEEFAFVGREH---LLDEASVHVERAVSGGSGALLIGGEPGIGKTRLAAEIARSSQRTHGLMALAGRCDRHLASSLQPWLEAL 606
            G  + +EP+  DVL +L+EHR+RVVSKEELLD +WGDRFVSE+A+TT ++ AR A+GD GR QRLIR    RGYQF+G V  +        P A+   +  DV +P      + AA   L       F GR+H   +L +A   V    SG    +L+ GE GIGKT L +  A +    H  + L GRCD  L+   QPW E L
Sbjct:   24 GKRIPVEPRALDVLHHLIEHRDRVVSKEELLDAVWGDRFVSEAAVTTALRTARMAIGDTGRQQRLIRTVFRRGYQFVGTVEQA--------PAADGPGTRRDVPLP-----ARLAASSGL------GFAGRDHERVVLADAWKDV--LASGRRRVVLVSGEAGIGKTTLCSVFAAAKH--HEAVVLYGRCDEELSIPYQPWREVL 205          
BLAST of mRNA_M-pyrifera_M_contig91760.21250.1 vs. uniprot
Match: A0A8J3ZQ62_9ACTN (OmpR/PhoB-type domain-containing protein n=1 Tax=Virgisporangium ochraceum TaxID=65505 RepID=A0A8J3ZQ62_9ACTN)

HSP 1 Score: 130 bits (327), Expect = 1.730e-31
Identity = 82/170 (48.24%), Postives = 97/170 (57.06%), Query Frame = 1
Query:   16 IEPQVFDVLAYLVEHRERVVSKEELLDEIWGDRFVSESALTTRIKMARRAVGDDGRAQRLIRNSHGRGYQFIGEVVSSAAREPNERPIAESSQSEPDVRVPPAASPTKQAADFALLVDEEFAFVGREHLLDEASVHVERAVSGGSGALLI----GGEPGIGKTRLAAEIA 513
            +EPQVF VLA L+  R RVVSKEELLD +WG+RFVSESALT+RIK ARRA+GDDGR QRLIR  HGRGYQF+GEVV   A       +A      P     P A  T   A   + V     F GR   LD     +     G + + ++     G PG+GKT LA   A
Sbjct:   26 VEPQVFSVLAMLLRERHRVVSKEELLDAVWGNRFVSESALTSRIKSARRAIGDDGRGQRLIRTVHGRGYQFVGEVVERGAA------VA------PVAAAVPVARTTTGPAQLPMAVR---GFAGRAEELDRLDALLHGTAPGAAPSTVVISAVSGAPGVGKTSLAVHWA 180          
BLAST of mRNA_M-pyrifera_M_contig91760.21250.1 vs. uniprot
Match: A0A5M3WXI6_9ACTN (Transcriptional regulator n=1 Tax=Acrocarpospora macrocephala TaxID=150177 RepID=A0A5M3WXI6_9ACTN)

HSP 1 Score: 124 bits (312), Expect = 1.750e-30
Identity = 59/84 (70.24%), Postives = 71/84 (84.52%), Query Frame = 1
Query:    1 GVAVRIEPQVFDVLAYLVEHRERVVSKEELLDEIWGDRFVSESALTTRIKMARRAVGDDGRAQRLIRNSHGRGYQFIGEVVSSA 252
            G  VR+EPQVFD+LA L+  R+RVVSKEELLD +WG+RFV+ESALT+R+K ARRA+GDDGR QRLIR  HGRGYQF+G V+  A
Sbjct:   21 GQVVRVEPQVFDLLAVLLRLRQRVVSKEELLDAVWGNRFVTESALTSRVKAARRAIGDDGRGQRLIRTVHGRGYQFVGTVIERA 104          
BLAST of mRNA_M-pyrifera_M_contig91760.21250.1 vs. uniprot
Match: UPI0018DFFDEB (alpha/beta hydrolase n=1 Tax=Nocardioides sp. CGMCC 1.13656 TaxID=2755556 RepID=UPI0018DFFDEB)

HSP 1 Score: 123 bits (309), Expect = 4.690e-30
Identity = 58/85 (68.24%), Postives = 71/85 (83.53%), Query Frame = 1
Query:    1 GVAVRIEPQVFDVLAYLVEHRERVVSKEELLDEIWGDRFVSESALTTRIKMARRAVGDDGRAQRLIRNSHGRGYQFIGEVVSSAA 255
            GVAV +EPQVFDVLA+L+ HR+RVV+K ELLDE+WGDRFVS++ LT+R+K AR+AVGDDGR QRLI   HG GY+F G+VV   A
Sbjct:   20 GVAVHVEPQVFDVLAHLIRHRDRVVTKTELLDEVWGDRFVSDATLTSRLKAARQAVGDDGRGQRLIATVHGVGYRFCGDVVGEEA 104          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig91760.21250.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7Y2ICZ1_9ACTN6.920e-3643.22AAA family ATPase n=1 Tax=Acidimicrobiales bacteri... [more]
UPI001B359D071.300e-3543.84AAA family ATPase n=2 Tax=Micromonospora sp. D93 T... [more]
A0A848UJF2_9ACTN1.380e-3545.73AAA family ATPase (Fragment) n=1 Tax=Ilumatobacter... [more]
A0A4R4MSF9_9ACTN8.270e-3543.84OmpR/PhoB-type domain-containing protein n=2 Tax=A... [more]
A0A1C5JKI6_9ACTN1.530e-3444.83Transcriptional regulatory protein, C terminal n=1... [more]
A0A6N7FR29_9ACTN1.640e-3471.43Alpha/beta fold hydrolase n=1 Tax=Acidimicrobiia b... [more]
A0A239F314_9ACTN9.780e-3443.41Transcriptional regulatory protein, C terminal n=3... [more]
A0A8J3ZQ62_9ACTN1.730e-3148.24OmpR/PhoB-type domain-containing protein n=1 Tax=V... [more]
A0A5M3WXI6_9ACTN1.750e-3070.24Transcriptional regulator n=1 Tax=Acrocarpospora m... [more]
UPI0018DFFDEB4.690e-3068.24alpha/beta hydrolase n=1 Tax=Nocardioides sp. CGMC... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig91760contigM-pyrifera_M_contig91760:136..741 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score117.1
Seed ortholog evalue9.6e-24
Seed eggNOG ortholog1120949.KB903339_gene8070
Preferred namelipJ
Hectar predicted targeting categoryother localisation
EggNOG free text desc.carboxylic ester hydrolase activity
EggNOG OGs2I75H@201174,COG2267@1,COG2267@2,COG3710@1,COG3710@2
COG Functional cat.I
Best tax levelActinobacteria
Best eggNOG OGNA|NA|NA
Exons1
Model size606
Cds size606
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816830.344849-CDS-M-pyrifera_M_contig91760:135..7411622816830.344849-CDS-M-pyrifera_M_contig91760:135..741Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91760 136..741 -
1692278203.6544375-CDS-M-pyrifera_M_contig91760:135..7411692278203.6544375-CDS-M-pyrifera_M_contig91760:135..741Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91760 136..741 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig91760.21250.1prot_M-pyrifera_M_contig91760.21250.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig91760 136..741 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig91760.21250.1

>prot_M-pyrifera_M_contig91760.21250.1 ID=prot_M-pyrifera_M_contig91760.21250.1|Name=mRNA_M-pyrifera_M_contig91760.21250.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=202bp
GVAVRIEPQVFDVLAYLVEHRERVVSKEELLDEIWGDRFVSESALTTRIK
MARRAVGDDGRAQRLIRNSHGRGYQFIGEVVSSAAREPNERPIAESSQSE
PDVRVPPAASPTKQAADFALLVDEEFAFVGREHLLDEASVHVERAVSGGS
GALLIGGEPGIGKTRLAAEIARSSQRTHGLMALAGRCDRHLASSLQPWLE
AL
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mRNA from alignment at M-pyrifera_M_contig91760:136..741-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig91760.21250.1 ID=mRNA_M-pyrifera_M_contig91760.21250.1|Name=mRNA_M-pyrifera_M_contig91760.21250.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=606bp|location=Sequence derived from alignment at M-pyrifera_M_contig91760:136..741- (Macrocystis pyrifera P11B4 male)
GGTGTGGCCGTTCGGATCGAACCCCAGGTCTTCGACGTCCTGGCCTACTT GGTCGAGCACCGCGAGCGAGTCGTCTCGAAGGAAGAGCTCCTCGACGAGA TCTGGGGTGATCGCTTCGTCAGCGAGTCGGCCCTGACCACCCGAATCAAG ATGGCTCGACGAGCGGTGGGCGACGACGGCCGAGCGCAACGGCTGATTCG CAACTCCCACGGCCGGGGCTACCAGTTCATCGGTGAGGTCGTGTCCAGCG CCGCTCGTGAACCGAACGAGCGCCCGATCGCAGAGTCCAGCCAGTCAGAG CCCGACGTTCGAGTGCCCCCGGCTGCCTCACCGACCAAGCAAGCCGCCGA CTTTGCGTTGCTCGTCGACGAAGAGTTCGCCTTTGTCGGTCGCGAACACC TGCTCGACGAGGCGAGTGTGCACGTCGAGCGAGCCGTCTCCGGCGGCAGT GGAGCCTTGCTGATCGGTGGCGAGCCAGGAATCGGCAAGACGAGGCTCGC CGCCGAGATCGCTCGGAGCAGTCAACGCACCCATGGCCTGATGGCGTTGG CAGGTCGCTGCGACCGCCACCTCGCCTCGTCGCTCCAGCCCTGGCTCGAA GCGCTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig91760:136..741-

>mRNA_M-pyrifera_M_contig91760.21250.1 ID=mRNA_M-pyrifera_M_contig91760.21250.1|Name=mRNA_M-pyrifera_M_contig91760.21250.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1212bp|location=Sequence derived from alignment at M-pyrifera_M_contig91760:136..741- (Macrocystis pyrifera P11B4 male)
GGTGTGGCCGTTCGGATCGAACCCCAGGTCTTCGACGTCCTGGCCTACTT
GGTCGAGCACCGCGAGCGAGTCGTCTCGAAGGAAGAGCTCCTCGACGAGA
TCTGGGGTGATCGCTTCGTCAGCGAGTCGGCCCTGACCACCCGAATCAAG
ATGGCTCGACGAGCGGTGGGCGACGACGGCCGAGCGCAACGGCTGATTCG
CAACTCCCACGGCCGGGGCTACCAGTTCATCGGTGAGGTCGTGTCCAGCG
CCGCTCGTGAACCGAACGAGCGCCCGATCGCAGAGTCCAGCCAGTCAGAG
CCCGACGTTCGAGTGCCCCCGGCTGCCTCACCGACCAAGCAAGCCGCCGA
CTTTGCGTTGCTCGTCGACGAAGAGTTCGCCTTTGTCGGTCGCGAACACC
TGCTCGACGAGGCGAGTGTGCACGTCGAGCGAGCCGTCTCCGGCGGCAGT
GGAGCCTTGCTGATCGGTGGCGAGCCAGGAATCGGCAAGACGAGGCTCGC
CGCCGAGATCGCTCGGAGCAGTCAACGCACCCATGGCCTGATGGCGTTGG
CAGGTCGCTGCGACCGCCACCTCGCCTCGTCGCTCCAGCCCTGGCTCGAA
GCGCTCGGTGTGGCCGTTCGGATCGAACCCCAGGTCTTCGACGTCCTGGC
CTACTTGGTCGAGCACCGCGAGCGAGTCGTCTCGAAGGAAGAGCTCCTCG
ACGAGATCTGGGGTGATCGCTTCGTCAGCGAGTCGGCCCTGACCACCCGA
ATCAAGATGGCTCGACGAGCGGTGGGCGACGACGGCCGAGCGCAACGGCT
GATTCGCAACTCCCACGGCCGGGGCTACCAGTTCATCGGTGAGGTCGTGT
CCAGCGCCGCTCGTGAACCGAACGAGCGCCCGATCGCAGAGTCCAGCCAG
TCAGAGCCCGACGTTCGAGTGCCCCCGGCTGCCTCACCGACCAAGCAAGC
CGCCGACTTTGCGTTGCTCGTCGACGAAGAGTTCGCCTTTGTCGGTCGCG
AACACCTGCTCGACGAGGCGAGTGTGCACGTCGAGCGAGCCGTCTCCGGC
GGCAGTGGAGCCTTGCTGATCGGTGGCGAGCCAGGAATCGGCAAGACGAG
GCTCGCCGCCGAGATCGCTCGGAGCAGTCAACGCACCCATGGCCTGATGG
CGTTGGCAGGTCGCTGCGACCGCCACCTCGCCTCGTCGCTCCAGCCCTGG
CTCGAAGCGCTC
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