mRNA_M-pyrifera_M_contig91447.21194.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig91447.21194.1
Unique NamemRNA_M-pyrifera_M_contig91447.21194.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig91447.21194.1 vs. uniprot
Match: A0A2R5GVS7_9STRA (tRNA pseudouridine synthase 1 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GVS7_9STRA)

HSP 1 Score: 107 bits (268), Expect = 4.180e-25
Identity = 54/110 (49.09%), Postives = 76/110 (69.09%), Query Frame = 1
Query:   13 PNAVTVESVLEDALRKCGLLELEEGKDWTEVGYSRAARTDKGVHAAGNVVALMMRLRPEDVKAKM--EELNALLPPTMVVLDCKRVSKRFNARSACTSRRYEYLLPTFCL 336
            P  VT+ES LEDA+ K G++  E   +  +VG+SRAARTDK VHA G VV+L + ++PE+ + +     LNA LP ++ V   +R +K FNA+SAC+ R+YEYL+PTF L
Sbjct:   90 PEVVTIESTLEDAITKTGVIRKENEGELQKVGWSRAARTDKSVHALGQVVSLQLLVKPEEAEIEKFRAALNAQLPDSIRVFGVQRTTKSFNAKSACSGRKYEYLIPTFML 199          
BLAST of mRNA_M-pyrifera_M_contig91447.21194.1 vs. uniprot
Match: A0A1X2H820_SYNRA (Pseudouridine synthase n=1 Tax=Syncephalastrum racemosum TaxID=13706 RepID=A0A1X2H820_SYNRA)

HSP 1 Score: 105 bits (263), Expect = 5.810e-25
Identity = 56/111 (50.45%), Postives = 74/111 (66.67%), Query Frame = 1
Query:   10 NPNAVTVESVLEDALRKCGLLELEEGKDWTEVGYSRAARTDKGVHAAGNVVALMMRLRPEDVKAKMEELNALLPPTMVVLDCKRVSKRFNARSACTSRRYEYLLPTFCLKP 342
            NP+A T+E  L  AL K G +  +   D  +V + RAARTDKGVHAAGN+V+L M L P  V+  +  +NA LPPT+ V D  RV   FNA+ +C+SR+YEYLLPT+  +P
Sbjct:   16 NPDARTIEETLFGALCKTGAVSQDNASDLKKVQFLRAARTDKGVHAAGNLVSLKM-LVPGTVQDMITSINAQLPPTIRVWDAIRVQNSFNAKRSCSSRKYEYLLPTYVFRP 125          
BLAST of mRNA_M-pyrifera_M_contig91447.21194.1 vs. uniprot
Match: B9WIU7_CANDC (tRNA pseudouridine synthase, putative n=1 Tax=Candida dubliniensis (strain CD36 / ATCC MYA-646 / CBS 7987 / NCPF 3949 / NRRL Y-17841) TaxID=573826 RepID=B9WIU7_CANDC)

HSP 1 Score: 99.8 bits (247), Expect = 3.310e-22
Identity = 51/114 (44.74%), Postives = 72/114 (63.16%), Query Frame = 1
Query:    1 VCRNPNAVTVESVLEDALRKCGLLELEEGKDWTEVGYSRAARTDKGVHAAGNVVALMMRLRPEDVKAKMEELNALLPPTMVVLDCKRVSKRFNARSACTSRRYEYLLPTFCLKP 342
            V  +PN  T+E  + DA+ K G + LE   D  + G+ RAARTDKGVHAAGNV++L M +   ++   + ++N LLP  + +   +R +K F+ R  C+SR YEYLLPTF L P
Sbjct:   57 VQNDPNVKTIEKDIYDAMTKAGAISLENSVDLKKSGFQRAARTDKGVHAAGNVISLKMIIEDPNI---INKINDLLPKQIRIWGIQRTTKGFDCRKCCSSRIYEYLLPTFSLLP 167          
BLAST of mRNA_M-pyrifera_M_contig91447.21194.1 vs. uniprot
Match: A0A7S0NK21_MICPS (Hypothetical protein (Fragment) n=1 Tax=Micromonas pusilla TaxID=38833 RepID=A0A7S0NK21_MICPS)

HSP 1 Score: 96.3 bits (238), Expect = 4.450e-22
Identity = 53/112 (47.32%), Postives = 68/112 (60.71%), Query Frame = 1
Query:    7 RNPNAVTVESVLEDALRKCGLLELEEGKDWTEVGYSRAARTDKGVHAAGNVVALMMRLRPEDVKAKMEELNALLPPTMVVLDCKRVSKRFNARSACTSRRYEYLLPTFCLKP 342
            RNP A TVE  LE A+   G +  +   D+T+V ++RAARTDKGV A G  VAL M L P  V   +E +NA LP    +   KRV+  FNA++ C  RRYEY++PTF   P
Sbjct:  116 RNPGARTVEDELERAIVAAGGISADNAGDFTKVQWTRAARTDKGVSAVGQCVALRMVLDPPGV---LERINAALPDGFKIFGYKRVTGGFNAKTMCDRRRYEYVIPTFAFDP 224          
BLAST of mRNA_M-pyrifera_M_contig91447.21194.1 vs. uniprot
Match: W6MM72_9ASCO (PseudoU_synth_1 domain-containing protein n=1 Tax=Kuraishia capsulata CBS 1993 TaxID=1382522 RepID=W6MM72_9ASCO)

HSP 1 Score: 96.7 bits (239), Expect = 4.850e-21
Identity = 51/111 (45.95%), Postives = 71/111 (63.96%), Query Frame = 1
Query:   10 NPNAVTVESVLEDALRKCGLLELEEGKDWTEVGYSRAARTDKGVHAAGNVVALMMRLRPEDVKAKMEELNALLPPTMVVLDCKRVSKRFNARSACTSRRYEYLLPTFCLKP 342
            NP   T+E  L +A  K G +  +   D  +  + RAARTDKGVHAAGNVV+L M +  +++   +E+LNA LP  + V   +RV+K F+ R  C+SR YEYL+P+FCL P
Sbjct:  109 NPGTKTIEYELFEAFVKTGAISRDNSNDLKKSSFMRAARTDKGVHAAGNVVSLKMIVEDDNL---LEKLNAELPDQIRVWGIERVNKSFDCRKMCSSRVYEYLMPSFCLLP 216          
BLAST of mRNA_M-pyrifera_M_contig91447.21194.1 vs. uniprot
Match: A0A1E4T1Q6_9ASCO (PseudoU_synth_1 domain-containing protein n=1 Tax=[Candida] arabinofermentans NRRL YB-2248 TaxID=983967 RepID=A0A1E4T1Q6_9ASCO)

HSP 1 Score: 96.3 bits (238), Expect = 6.190e-21
Identity = 51/111 (45.95%), Postives = 69/111 (62.16%), Query Frame = 1
Query:   10 NPNAVTVESVLEDALRKCGLLELEEGKDWTEVGYSRAARTDKGVHAAGNVVALMMRLRPEDVKAKMEELNALLPPTMVVLDCKRVSKRFNARSACTSRRYEYLLPTFCLKP 342
            NP A T+E  L DA  K G +      D  + G+ RAARTDKGVHAAGNVV+L M +  ED+   +E++N+ LP  + +   +RV+K F+ R  C SR YEYL+PT+   P
Sbjct:   97 NPPARTIEGDLFDAFVKAGAISKNNSNDLKKSGFQRAARTDKGVHAAGNVVSLKMIIEDEDI---VEKINSYLPDQIRLWGYERVNKSFDCRKMCGSRVYEYLMPTYSFLP 204          
BLAST of mRNA_M-pyrifera_M_contig91447.21194.1 vs. uniprot
Match: A0A507FC33_9FUNG (PseudoU_synth_1 domain-containing protein n=1 Tax=Chytriomyces confervae TaxID=246404 RepID=A0A507FC33_9FUNG)

HSP 1 Score: 95.9 bits (237), Expect = 6.790e-21
Identity = 56/110 (50.91%), Postives = 70/110 (63.64%), Query Frame = 1
Query:   10 NPNAVTVESVLEDALRKCGLLELEEGKDWTEVGYSRAARTDKGVHAAGNVVALMMRLRPEDVKAKMEELNALLPPTMVVLDCKRVSKRFNARSACTSRRYEYLLPTFCLK 339
            NP   T+ES    A  K G +  E+  D  +VG +R ARTDKGVHAAGNVV+L MR  P  V+A    LNA LPP + V DC RV+  FNA++ C  R+YEY +PT+ LK
Sbjct:   80 NPPHDTIESEFMKAAVKAGSVS-EDNIDPKKVGLNRCARTDKGVHAAGNVVSLKMRAIPNLVEA----LNAHLPPQIRVYDCLRVTGSFNAKNHCGGRQYEYFMPTYILK 184          
BLAST of mRNA_M-pyrifera_M_contig91447.21194.1 vs. uniprot
Match: C4YSR3_CANAW (tRNA pseudouridine synthase 1 n=1 Tax=Candida albicans (strain WO-1) TaxID=294748 RepID=C4YSR3_CANAW)

HSP 1 Score: 95.9 bits (237), Expect = 7.140e-21
Identity = 49/114 (42.98%), Postives = 70/114 (61.40%), Query Frame = 1
Query:    1 VCRNPNAVTVESVLEDALRKCGLLELEEGKDWTEVGYSRAARTDKGVHAAGNVVALMMRLRPEDVKAKMEELNALLPPTMVVLDCKRVSKRFNARSACTSRRYEYLLPTFCLKP 342
            V  +PN  T+E  + DA+   G +  E   D  + G+ RAARTDKGVHAAGNV++L M +   ++   + ++N LLP  + +   +R +K F+ R  C+SR YEYLLPTF L P
Sbjct:   14 VQNDPNVKTIEKDIYDAMATAGAISAENAVDLKKSGFQRAARTDKGVHAAGNVISLKMIIEDPEI---INKINDLLPKQIRIWGIQRTTKGFDCRKCCSSRIYEYLLPTFSLLP 124          
BLAST of mRNA_M-pyrifera_M_contig91447.21194.1 vs. uniprot
Match: A0A1B2J7N2_PICPA (BA75_00852T0 n=3 Tax=Komagataella TaxID=460517 RepID=A0A1B2J7N2_PICPA)

HSP 1 Score: 95.9 bits (237), Expect = 8.680e-21
Identity = 49/111 (44.14%), Postives = 69/111 (62.16%), Query Frame = 1
Query:   10 NPNAVTVESVLEDALRKCGLLELEEGKDWTEVGYSRAARTDKGVHAAGNVVALMMRLRPEDVKAKMEELNALLPPTMVVLDCKRVSKRFNARSACTSRRYEYLLPTFCLKP 342
            NP   T+E  L +A    G +  +   D  +  + RAARTDKGVHAAGNV++L M +  ED+   +E++N+ LPPT+ V   +R +K F+ R  C+SR YEYL+PTF   P
Sbjct:  109 NPPQKTIEGDLFEAFVASGAISRDNSNDLKKSSFMRAARTDKGVHAAGNVISLKMIIEDEDI---LEKINSKLPPTIRVWGYERTNKNFDCRKMCSSRIYEYLMPTFSFLP 216          
BLAST of mRNA_M-pyrifera_M_contig91447.21194.1 vs. uniprot
Match: A0A167S5Y8_CALVF (Pseudouridine synthase n=1 Tax=Calocera viscosa (strain TUFC12733) TaxID=1330018 RepID=A0A167S5Y8_CALVF)

HSP 1 Score: 95.9 bits (237), Expect = 9.480e-21
Identity = 49/111 (44.14%), Postives = 69/111 (62.16%), Query Frame = 1
Query:   10 NPNAVTVESVLEDALRKCGLLELEEGKDWTEVGYSRAARTDKGVHAAGNVVALMMRLRPEDVKAKMEELNALLPPTMVVLDCKRVSKRFNARSACTSRRYEYLLPTFCLKP 342
            NP A T+E +L +AL K G +  +   D  +VG +RAARTD GVHAAGN+V++ +   PE V+  +  +N  LPP + +    R +  FNAR +C SR YEYL P++ L P
Sbjct:  144 NPPARTIEGILFEALIKAGCVSADNSDDPNKVGLTRAARTDAGVHAAGNIVSMKIIREPEGVEDLVATVNTHLPPEVRLWGTVRTANNFNARMSCDSRVYEYLFPSYILIP 254          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig91447.21194.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2R5GVS7_9STRA4.180e-2549.09tRNA pseudouridine synthase 1 n=1 Tax=Hondaea ferm... [more]
A0A1X2H820_SYNRA5.810e-2550.45Pseudouridine synthase n=1 Tax=Syncephalastrum rac... [more]
B9WIU7_CANDC3.310e-2244.74tRNA pseudouridine synthase, putative n=1 Tax=Cand... [more]
A0A7S0NK21_MICPS4.450e-2247.32Hypothetical protein (Fragment) n=1 Tax=Micromonas... [more]
W6MM72_9ASCO4.850e-2145.95PseudoU_synth_1 domain-containing protein n=1 Tax=... [more]
A0A1E4T1Q6_9ASCO6.190e-2145.95PseudoU_synth_1 domain-containing protein n=1 Tax=... [more]
A0A507FC33_9FUNG6.790e-2150.91PseudoU_synth_1 domain-containing protein n=1 Tax=... [more]
C4YSR3_CANAW7.140e-2142.98tRNA pseudouridine synthase 1 n=1 Tax=Candida albi... [more]
A0A1B2J7N2_PICPA8.680e-2144.14BA75_00852T0 n=3 Tax=Komagataella TaxID=460517 Rep... [more]
A0A167S5Y8_CALVF9.480e-2144.14Pseudouridine synthase n=1 Tax=Calocera viscosa (s... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig91447contigM-pyrifera_M_contig91447:28..369 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score99.4
Seed ortholog evalue1.2e-18
Seed eggNOG ortholog42374.XP_002421009.1
Preferred namePUS1
KEGG koko:K06173,ko:K15452
Hectar predicted targeting categoryother localisation
GOsGO:0001522,GO:0003674,GO:0003676,GO:0003723,GO:0003729,GO:0003824,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0006139,GO:0006396,GO:0006399,GO:0006400,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0009451,GO:0009982,GO:0009987,GO:0010467,GO:0016070,GO:0016071,GO:0016073,GO:0016556,GO:0016853,GO:0016866,GO:0031119,GO:0031120,GO:0034470,GO:0034641,GO:0034660,GO:0040031,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0071704,GO:0090304,GO:0097159,GO:0106032,GO:0140098,GO:1901360,GO:1901363,GO:1990481
EggNOG free text desc.Pseudouridine synthase
EggNOG OGs38F38@33154,3NVYM@4751,3QNKA@4890,3RSJ4@4891,47BK1@766764,COG0101@1,KOG2553@2759
EC5.4.99.12,5.4.99.44
COG Functional cat.J
BiGG ReactioniMM904.YGL063W,iND750.YGL063W
Best tax levelDebaryomycetaceae
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03016
Exons1
Model size342
Cds size342
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816827.5419831-CDS-M-pyrifera_M_contig91447:27..3691622816827.5419831-CDS-M-pyrifera_M_contig91447:27..369Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91447 28..369 +
1692278202.0035322-CDS-M-pyrifera_M_contig91447:27..3691692278202.0035322-CDS-M-pyrifera_M_contig91447:27..369Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91447 28..369 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig91447.21194.1prot_M-pyrifera_M_contig91447.21194.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig91447 28..369 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig91447.21194.1

>prot_M-pyrifera_M_contig91447.21194.1 ID=prot_M-pyrifera_M_contig91447.21194.1|Name=mRNA_M-pyrifera_M_contig91447.21194.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=114bp
VCRNPNAVTVESVLEDALRKCGLLELEEGKDWTEVGYSRAARTDKGVHAA
GNVVALMMRLRPEDVKAKMEELNALLPPTMVVLDCKRVSKRFNARSACTS
RRYEYLLPTFCLKP
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mRNA from alignment at M-pyrifera_M_contig91447:28..369+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig91447.21194.1 ID=mRNA_M-pyrifera_M_contig91447.21194.1|Name=mRNA_M-pyrifera_M_contig91447.21194.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=342bp|location=Sequence derived from alignment at M-pyrifera_M_contig91447:28..369+ (Macrocystis pyrifera P11B4 male)
GTTTGCAGGAATCCGAATGCAGTGACGGTGGAGAGTGTGTTGGAAGATGC ATTGCGCAAGTGTGGTCTGTTGGAACTGGAGGAAGGGAAGGATTGGACAG AAGTAGGGTATAGCCGAGCTGCCAGGACCGATAAAGGTGTGCATGCCGCC GGAAATGTAGTAGCATTGATGATGCGGCTGCGACCGGAGGATGTGAAGGC GAAAATGGAGGAGTTGAATGCTCTTTTGCCCCCCACAATGGTGGTCCTTG ATTGCAAGCGCGTATCGAAGCGATTCAATGCTAGATCGGCGTGCACATCG AGGCGGTATGAGTATCTCCTTCCCACCTTTTGCCTCAAACCC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig91447:28..369+

>mRNA_M-pyrifera_M_contig91447.21194.1 ID=mRNA_M-pyrifera_M_contig91447.21194.1|Name=mRNA_M-pyrifera_M_contig91447.21194.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=684bp|location=Sequence derived from alignment at M-pyrifera_M_contig91447:28..369+ (Macrocystis pyrifera P11B4 male)
GTTTGCAGGAATCCGAATGCAGTGACGGTGGAGAGTGTGTTGGAAGATGC
ATTGCGCAAGTGTGGTCTGTTGGAACTGGAGGAAGGGAAGGATTGGACAG
AAGTAGGGTATAGCCGAGCTGCCAGGACCGATAAAGGTGTGCATGCCGCC
GGAAATGTAGTAGCATTGATGATGCGGCTGCGACCGGAGGATGTGAAGGC
GAAAATGGAGGAGTTGAATGCTCTTTTGCCCCCCACAATGGTGGTCCTTG
ATTGCAAGCGCGTATCGAAGCGATTCAATGCTAGATCGGCGTGCACATCG
AGGCGGTATGAGTATCTCCTTCCCACCTTTTGCCTCAAACCCGTTTGCAG
GAATCCGAATGCAGTGACGGTGGAGAGTGTGTTGGAAGATGCATTGCGCA
AGTGTGGTCTGTTGGAACTGGAGGAAGGGAAGGATTGGACAGAAGTAGGG
TATAGCCGAGCTGCCAGGACCGATAAAGGTGTGCATGCCGCCGGAAATGT
AGTAGCATTGATGATGCGGCTGCGACCGGAGGATGTGAAGGCGAAAATGG
AGGAGTTGAATGCTCTTTTGCCCCCCACAATGGTGGTCCTTGATTGCAAG
CGCGTATCGAAGCGATTCAATGCTAGATCGGCGTGCACATCGAGGCGGTA
TGAGTATCTCCTTCCCACCTTTTGCCTCAAACCC
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