mRNA_M-pyrifera_M_contig90440.20972.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig90440.20972.1
Unique NamemRNA_M-pyrifera_M_contig90440.20972.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig90440.20972.1 vs. uniprot
Match: A0A1F8V9V8_9COXI (Reverse transcriptase n=1 Tax=Coxiella sp. RIFCSPHIGHO2_12_FULL_42_15 TaxID=1797696 RepID=A0A1F8V9V8_9COXI)

HSP 1 Score: 102 bits (253), Expect = 5.530e-23
Identity = 50/112 (44.64%), Postives = 67/112 (59.82%), Query Frame = 1
Query:   10 LDQIIHIPIDRNAVITNPTCGIPIRSALSPFLAALYLSPLDRAFEAKACFYVRYCDDILILLPSLRAYQRARQTLFKILSDLKLTCAPSKTRMGRLHKGFHFLGLTCELAQT 345
            L+ II  PID      NP CGI +R  LS   +A+YL PLD AF+A    Y RY DDILIL  + + Y R R+ + ++L + +LT +  K+R GR+  GFHFLG+     QT
Sbjct:  164 LEDIIRNPIDTPRGFQNPECGIALRGPLSQLFSAIYLKPLDAAFDAMEVVYARYQDDILILCKTKQQYNRCRRKMSEVLQERRLTLSRKKSRFGRVEDGFHFLGINYLGTQT 275          
BLAST of mRNA_M-pyrifera_M_contig90440.20972.1 vs. uniprot
Match: A0A1G0GWJ5_9GAMM (Reverse transcriptase (Fragment) n=1 Tax=Gammaproteobacteria bacterium RIFCSPHIGHO2_12_FULL_40_19 TaxID=1798281 RepID=A0A1G0GWJ5_9GAMM)

HSP 1 Score: 95.9 bits (237), Expect = 5.680e-21
Identity = 47/112 (41.96%), Postives = 66/112 (58.93%), Query Frame = 1
Query:    1 LGYLDQIIHIPIDRNAVITNPTCGIPIRSALSPFLAALYLSPLDRAFEAKACFYVRYCDDILILLPSLRAYQRARQTLFKILSDLKLTCAPSKTRMGRLHKGFHFLGLTCEL 336
            +  L  II+ PID      NP  GI +R  LS F + +YL PLD AF+     Y+RY DDILIL  + R   R ++ +  +L + +L+ +  KTRMG +H GFHFLG++  L
Sbjct:  146 IAMLRNIINNPIDTPYGYKNPDFGIALRGPLSQFFSGIYLKPLDDAFDEMEVTYLRYQDDILILCHTKRQLNRCKRRMMMVLQERRLSLSKRKTRMGSIHAGFHFLGISYSL 257          
BLAST of mRNA_M-pyrifera_M_contig90440.20972.1 vs. uniprot
Match: G9EL80_9GAMM (Reverse transcriptase domain-containing protein n=9 Tax=Legionella TaxID=445 RepID=G9EL80_9GAMM)

HSP 1 Score: 95.1 bits (235), Expect = 2.340e-20
Identity = 48/105 (45.71%), Postives = 64/105 (60.95%), Query Frame = 1
Query:   10 LDQIIHIPIDRNAVITNPTCGIPIRSALSPFLAALYLSPLDRAFEAKACFYVRYCDDILILLPSLRAYQRARQTLFKILSDLKLTCAPSKTRMGRLHKGFHFLGL 324
            L++II  PI+      NP  GI +R  LS F +ALYL PLD AF      Y RY DDILIL  + R+  R +Q L ++L + +L  +  KTR+G + KGFHFLG+
Sbjct:  163 LERIIVNPIETPRGYKNPDTGIALRGPLSQFFSALYLKPLDDAFNEMGVAYFRYQDDILILCQTKRSLNRCKQRLMQVLQERRLGLSGKKTRIGSIDKGFHFLGI 267          
BLAST of mRNA_M-pyrifera_M_contig90440.20972.1 vs. uniprot
Match: UPI001A2D6180 (Reverse transcriptase n=1 Tax=Legionella pneumophila TaxID=446 RepID=UPI001A2D6180)

HSP 1 Score: 94.7 bits (234), Expect = 3.620e-20
Identity = 47/105 (44.76%), Postives = 66/105 (62.86%), Query Frame = 1
Query:   10 LDQIIHIPIDRNAVITNPTCGIPIRSALSPFLAALYLSPLDRAFEAKACFYVRYCDDILILLPSLRAYQRARQTLFKILSDLKLTCAPSKTRMGRLHKGFHFLGL 324
            L+ II  PI+      NP  GI +R  LS F +ALYL PLD AF+     Y+RY DDI+IL+ + R  +R ++ L ++L + KL  +  KTR+G + KGFHFLG+
Sbjct:  163 LENIIKNPIETPRGYKNPDYGIALRGPLSQFFSALYLKPLDDAFDRICGTYIRYQDDIIILVKTKRQLKRCKERLSQVLKERKLRLSRKKTRIGLIDKGFHFLGI 267          
BLAST of mRNA_M-pyrifera_M_contig90440.20972.1 vs. uniprot
Match: A0A0W0ZUA0_9GAMM (Reverse transcriptase (RNA-dependent DNA polymerase) n=1 Tax=Legionella tucsonensis TaxID=40335 RepID=A0A0W0ZUA0_9GAMM)

HSP 1 Score: 90.1 bits (222), Expect = 1.780e-19
Identity = 45/105 (42.86%), Postives = 63/105 (60.00%), Query Frame = 1
Query:   10 LDQIIHIPIDRNAVITNPTCGIPIRSALSPFLAALYLSPLDRAFEAKACFYVRYCDDILILLPSLRAYQRARQTLFKILSDLKLTCAPSKTRMGRLHKGFHFLGL 324
            L+QI+  PI+       P  GI +R  LS FL+ALYL PLD AF+     Y+RY DD++IL  S R  +R ++ +  IL +  L  +  KTR+G +  GFHFLG+
Sbjct:   67 LEQIVRNPIETPRGYKKPDNGIALRGPLSQFLSALYLKPLDDAFDTMDVTYLRYQDDLIILCHSKRQLERCKRRMMTILKERHLQLSRKKTRIGNIAGGFHFLGI 171          
BLAST of mRNA_M-pyrifera_M_contig90440.20972.1 vs. uniprot
Match: A0A447SZB1_9GAMM (Reverse transcriptase (RNA-dependent DNA polymerase) n=2 Tax=Legionella sainthelensi TaxID=28087 RepID=A0A447SZB1_9GAMM)

HSP 1 Score: 86.3 bits (212), Expect = 1.770e-17
Identity = 44/105 (41.90%), Postives = 61/105 (58.10%), Query Frame = 1
Query:   10 LDQIIHIPIDRNAVITNPTCGIPIRSALSPFLAALYLSPLDRAFEAKACFYVRYCDDILILLPSLRAYQRARQTLFKILSDLKLTCAPSKTRMGRLHKGFHFLGL 324
            L+ I+  PI+      NP  GI +R  LS F +ALYL PLD AF++    Y+RY DDI+I   + R  +R ++ L  IL    L  +  KTR+G +  GFHFLG+
Sbjct:   94 LENIVRNPIETPRGYKNPDNGIALRGPLSQFFSALYLRPLDDAFDSMDVTYLRYQDDIIIFAHTKRQLERCKRRLMTILKKRHLQLSRKKTRIGSIAGGFHFLGI 198          
BLAST of mRNA_M-pyrifera_M_contig90440.20972.1 vs. uniprot
Match: D3HTL9_LEGLN (Putative reverse transcriptase (RNA-dependent DNA polymerase) n=15 Tax=Legionella TaxID=445 RepID=D3HTL9_LEGLN)

HSP 1 Score: 86.7 bits (213), Expect = 3.690e-17
Identity = 44/98 (44.90%), Postives = 58/98 (59.18%), Query Frame = 1
Query:   31 PIDRNAVITNPTCGIPIRSALSPFLAALYLSPLDRAFEAKACFYVRYCDDILILLPSLRAYQRARQTLFKILSDLKLTCAPSKTRMGRLHKGFHFLGL 324
            PI+      NP  GI +R  LS F +ALYL PLD AF+     Y+RY DDI+IL  S R  +R ++ L  IL +  L  +  KTR+G +  GFHFLG+
Sbjct:  171 PIETPRGYKNPDNGIALRGPLSQFFSALYLKPLDDAFDTLDVTYLRYQDDIIILCHSKRQLERCKRRLMTILKERHLQLSRKKTRIGAIEGGFHFLGI 268          
BLAST of mRNA_M-pyrifera_M_contig90440.20972.1 vs. uniprot
Match: UPI001A268868 (Reverse transcriptase n=4 Tax=Legionella TaxID=445 RepID=UPI001A268868)

HSP 1 Score: 86.7 bits (213), Expect = 3.970e-17
Identity = 44/98 (44.90%), Postives = 58/98 (59.18%), Query Frame = 1
Query:   31 PIDRNAVITNPTCGIPIRSALSPFLAALYLSPLDRAFEAKACFYVRYCDDILILLPSLRAYQRARQTLFKILSDLKLTCAPSKTRMGRLHKGFHFLGL 324
            PI+      NP  GI +R  LS F +ALYL PLD AF+     Y+RY DDI+IL  S R  +R ++ L  IL +  L  +  KTR+G +  GFHFLG+
Sbjct:  171 PIETPRGYKNPDNGIALRGPLSQFFSALYLKPLDDAFDTLDVTYLRYQDDIIILCHSKRQLERCKRRLMTILKERHLQLSRKKTRIGAIEGGFHFLGI 268          
BLAST of mRNA_M-pyrifera_M_contig90440.20972.1 vs. uniprot
Match: A0A846CAB4_9CYAN (Reverse transcriptase domain-containing protein n=1 Tax=Symploca sp. SIO1B1 TaxID=2607763 RepID=A0A846CAB4_9CYAN)

HSP 1 Score: 83.2 bits (204), Expect = 1.000e-16
Identity = 48/151 (31.79%), Postives = 72/151 (47.68%), Query Frame = 1
Query:   64 TCGIPIRSALSPFLAALYLSPLDRAFEAKACFYVRYCDDILILLPSLRAYQRARQTLFKILSDLKLTCAPSKTRMGRLHKGFHFLGLTCELAQTTRLKSGEAWPKPPQTQRPAVTLHQRTYHRAMEKLGALNTHAIAVAPTQAYLLRWSRW 516
            T GI +   LSP + AL+L PLD       CFYVRY DD +IL P+    ++A     +++S+L++   P KT +G++ +GF FLG  C  A                     + + ++T  R +E +  L          +AY LRW RW
Sbjct:   36 TQGISLGCPLSPLMGALFLKPLDDRMAELGCFYVRYMDDWVILAPTRWKLRKAILATNQVMSELRVIKHPEKTFIGKVARGFDFLGYWCSPA--------------------GLGIARKTVERMVENMRRLYERGAPDERIEAYFLRWCRW 166          
BLAST of mRNA_M-pyrifera_M_contig90440.20972.1 vs. uniprot
Match: A0A6P0MAV1_9CYAN (Reverse transcriptase (Fragment) n=1 Tax=Moorena sp. SIO3G5 TaxID=2607837 RepID=A0A6P0MAV1_9CYAN)

HSP 1 Score: 84.0 bits (206), Expect = 2.090e-16
Identity = 46/149 (30.87%), Postives = 73/149 (48.99%), Query Frame = 1
Query:   70 GIPIRSALSPFLAALYLSPLDRAFEAKACFYVRYCDDILILLPSLRAYQRARQTLFKILSDLKLTCAPSKTRMGRLHKGFHFLGLTCELAQTTRLKSGEAWPKPPQTQRPAVTLHQRTYHRAMEKLGALNTHAIAVAPTQAYLLRWSRW 516
            GI +   +SP + AL+L PLD       CFYVRY DD +IL P+    ++A +   +++++LK+   P KT +GR+ +GF FLG     +                     + + ++T  R +E +  L  HA  +   +AY  RW RW
Sbjct:  107 GISLGCPISPLIGALFLKPLDDRMARLGCFYVRYMDDWVILAPTRWKLRKAIKATNQVMNELKVLKHPDKTFIGRIVRGFDFLGFWFSTS--------------------GLGIARQTVERMLENVARLYEHAAPIERIEAYFKRWWRW 235          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig90440.20972.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1F8V9V8_9COXI5.530e-2344.64Reverse transcriptase n=1 Tax=Coxiella sp. RIFCSPH... [more]
A0A1G0GWJ5_9GAMM5.680e-2141.96Reverse transcriptase (Fragment) n=1 Tax=Gammaprot... [more]
G9EL80_9GAMM2.340e-2045.71Reverse transcriptase domain-containing protein n=... [more]
UPI001A2D61803.620e-2044.76Reverse transcriptase n=1 Tax=Legionella pneumophi... [more]
A0A0W0ZUA0_9GAMM1.780e-1942.86Reverse transcriptase (RNA-dependent DNA polymeras... [more]
A0A447SZB1_9GAMM1.770e-1741.90Reverse transcriptase (RNA-dependent DNA polymeras... [more]
D3HTL9_LEGLN3.690e-1744.90Putative reverse transcriptase (RNA-dependent DNA ... [more]
UPI001A2688683.970e-1744.90Reverse transcriptase n=4 Tax=Legionella TaxID=445... [more]
A0A846CAB4_9CYAN1.000e-1631.79Reverse transcriptase domain-containing protein n=... [more]
A0A6P0MAV1_9CYAN2.090e-1630.87Reverse transcriptase (Fragment) n=1 Tax=Moorena s... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig90440contigM-pyrifera_M_contig90440:2..526 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score92.4
Seed ortholog evalue2.2e-16
Seed eggNOG ortholog658187.LDG_6139
Hectar predicted targeting categoryother localisation
EggNOG free text desc.reverse transcriptase
EggNOG OGs1JC5F@118969,1PCD5@1224,1SXBQ@1236,COG3344@1,COG3344@2
COG Functional cat.L
Best tax levelLegionellales
Best eggNOG OGNA|NA|NA
Exons1
Model size525
Cds size525
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816816.5391018-CDS-M-pyrifera_M_contig90440:1..5261622816816.5391018-CDS-M-pyrifera_M_contig90440:1..526Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90440 2..526 +
1692278195.4393678-CDS-M-pyrifera_M_contig90440:1..5261692278195.4393678-CDS-M-pyrifera_M_contig90440:1..526Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90440 2..526 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig90440.20972.1prot_M-pyrifera_M_contig90440.20972.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig90440 2..526 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig90440.20972.1

>prot_M-pyrifera_M_contig90440.20972.1 ID=prot_M-pyrifera_M_contig90440.20972.1|Name=mRNA_M-pyrifera_M_contig90440.20972.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=175bp
LGYLDQIIHIPIDRNAVITNPTCGIPIRSALSPFLAALYLSPLDRAFEAK
ACFYVRYCDDILILLPSLRAYQRARQTLFKILSDLKLTCAPSKTRMGRLH
KGFHFLGLTCELAQTTRLKSGEAWPKPPQTQRPAVTLHQRTYHRAMEKLG
ALNTHAIAVAPTQAYLLRWSRWWAR
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mRNA from alignment at M-pyrifera_M_contig90440:2..526+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig90440.20972.1 ID=mRNA_M-pyrifera_M_contig90440.20972.1|Name=mRNA_M-pyrifera_M_contig90440.20972.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=525bp|location=Sequence derived from alignment at M-pyrifera_M_contig90440:2..526+ (Macrocystis pyrifera P11B4 male)
CTCGGTTATCTTGATCAGATTATTCACATTCCTATTGATCGAAACGCAGT GATCACTAATCCGACCTGTGGTATACCCATTCGCAGTGCTTTATCTCCTT TTCTGGCTGCGCTCTACCTCTCTCCACTAGACCGAGCCTTTGAGGCTAAA GCTTGCTTCTATGTGCGTTACTGTGATGACATCTTGATCTTGCTGCCTTC CCTAAGAGCCTACCAACGAGCCAGGCAAACACTGTTTAAGATCCTGTCTG ATCTTAAACTGACTTGTGCCCCAAGCAAAACACGTATGGGTAGGCTCCAT AAAGGCTTCCATTTTTTAGGTCTTACCTGCGAGTTGGCACAAACCACTCG TCTTAAGTCTGGGGAGGCTTGGCCAAAGCCTCCCCAAACTCAACGCCCGG CGGTCACGCTACATCAACGTACGTACCATCGAGCTATGGAGAAGCTTGGG GCTCTGAATACCCATGCCATCGCTGTGGCGCCTACACAGGCCTATCTTCT CCGCTGGTCCCGCTGGTGGGCTCGC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig90440:2..526+

>mRNA_M-pyrifera_M_contig90440.20972.1 ID=mRNA_M-pyrifera_M_contig90440.20972.1|Name=mRNA_M-pyrifera_M_contig90440.20972.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1050bp|location=Sequence derived from alignment at M-pyrifera_M_contig90440:2..526+ (Macrocystis pyrifera P11B4 male)
CTCGGTTATCTTGATCAGATTATTCACATTCCTATTGATCGAAACGCAGT
GATCACTAATCCGACCTGTGGTATACCCATTCGCAGTGCTTTATCTCCTT
TTCTGGCTGCGCTCTACCTCTCTCCACTAGACCGAGCCTTTGAGGCTAAA
GCTTGCTTCTATGTGCGTTACTGTGATGACATCTTGATCTTGCTGCCTTC
CCTAAGAGCCTACCAACGAGCCAGGCAAACACTGTTTAAGATCCTGTCTG
ATCTTAAACTGACTTGTGCCCCAAGCAAAACACGTATGGGTAGGCTCCAT
AAAGGCTTCCATTTTTTAGGTCTTACCTGCGAGTTGGCACAAACCACTCG
TCTTAAGTCTGGGGAGGCTTGGCCAAAGCCTCCCCAAACTCAACGCCCGG
CGGTCACGCTACATCAACGTACGTACCATCGAGCTATGGAGAAGCTTGGG
GCTCTGAATACCCATGCCATCGCTGTGGCGCCTACACAGGCCTATCTTCT
CCGCTGGTCCCGCTGGTGGGCTCGCCTCGGTTATCTTGATCAGATTATTC
ACATTCCTATTGATCGAAACGCAGTGATCACTAATCCGACCTGTGGTATA
CCCATTCGCAGTGCTTTATCTCCTTTTCTGGCTGCGCTCTACCTCTCTCC
ACTAGACCGAGCCTTTGAGGCTAAAGCTTGCTTCTATGTGCGTTACTGTG
ATGACATCTTGATCTTGCTGCCTTCCCTAAGAGCCTACCAACGAGCCAGG
CAAACACTGTTTAAGATCCTGTCTGATCTTAAACTGACTTGTGCCCCAAG
CAAAACACGTATGGGTAGGCTCCATAAAGGCTTCCATTTTTTAGGTCTTA
CCTGCGAGTTGGCACAAACCACTCGTCTTAAGTCTGGGGAGGCTTGGCCA
AAGCCTCCCCAAACTCAACGCCCGGCGGTCACGCTACATCAACGTACGTA
CCATCGAGCTATGGAGAAGCTTGGGGCTCTGAATACCCATGCCATCGCTG
TGGCGCCTACACAGGCCTATCTTCTCCGCTGGTCCCGCTGGTGGGCTCGC
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