mRNA_M-pyrifera_M_contig90217.20920.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig90217.20920.1
Unique NamemRNA_M-pyrifera_M_contig90217.20920.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig90217.20920.1 vs. uniprot
Match: A0A7I8VS01_9ANNE (DgyrCDS7167 n=1 Tax=Dimorphilus gyrociliatus TaxID=2664684 RepID=A0A7I8VS01_9ANNE)

HSP 1 Score: 122 bits (305), Expect = 1.230e-30
Identity = 77/212 (36.32%), Postives = 115/212 (54.25%), Query Frame = 1
Query:   22 FDAKPETWSKGFRLLLEAVVIVLPTLFVFTIGHSMVYEVVMFQTVAI--FVLGLTPLGQAGPVFHFDRLFDSTNAVAVINRNKKGTFRLRSPAMSLFRGGLLLGTALGILAVDFPAFPRFYAKTEDYGASLMDIGVGGFVFAAGFSAQRPS------------FTENLFHSLPVLGLGFARWLSVWLSDYHTHTSEYGRDLNFFFVLAFLQL 615
            F  K    + G + +L+  V+++P L   TIG + +YE+ +  T+ I  F+L +  L +AG            +    IN+  + TF    P ++ FR    +GTA+ ILA+DF   PR +AKTE++G  LMDIGVG FVFA G  + + +              +NL  SLP++GLGF R  S+  +DYH H +EYG   NFFF LA +++
Sbjct:   46 FLLKDAFINTGLKFILDFFVLIIPMLVASTIGSNFLYELYIITTLIIVGFLLVIPQLEKAGR--------KKISIKEEINKFFQDTFANEIPGITHFRSYANIGTAILILAIDFSVCPRRFAKTENFGTGLMDIGVGSFVFANGIVSPQATGKSGVDNSFWRNIKKNLISSLPLIGLGFGRLYSLKATDYHEHVTEYGIHWNFFFTLAIVKV 249          
BLAST of mRNA_M-pyrifera_M_contig90217.20920.1 vs. uniprot
Match: A0A218ZD83_9HELO (GPI-anchored wall transfer protein n=1 Tax=Marssonina coronariae TaxID=503106 RepID=A0A218ZD83_9HELO)

HSP 1 Score: 109 bits (272), Expect = 2.850e-24
Identity = 66/156 (42.31%), Postives = 86/156 (55.13%), Query Frame = 1
Query:  208 HFDRLFDSTNAVAVINRNKKGTFRLRS------PAMSLFRGGLLLGTALGILAVDFPAFPRFYAKTEDYGASLMDIGVGGFVFAAGFSAQRPSFT----------------ENLFHSLPVLGLGFARWLSVWLSDYHTHTSEYGRDLNFFFVLAFL 609
            H  +   S NA    +++  G F+ RS      P ++ +RG +++ T L ILAVDF  FPR +AK E++G SLMD+GVG FVF+AG  A RP                   ++L HSLP+L LGF R  SV   DY  H +EYG   NFFF L FL
Sbjct:  112 HLKKPKGSPNANIRQDKDSAGNFKARSNPLPKKPFLTTYRGSMIVITCLAILAVDFRIFPRRFAKVENWGTSLMDVGVGSFVFSAGIVATRPVLKARFAGTQAAPLSTRLYQSLRHSLPLLVLGFIRLYSVKGLDYAEHVTEYGVHWNFFFTLGFL 267          
BLAST of mRNA_M-pyrifera_M_contig90217.20920.1 vs. uniprot
Match: A0A2S6BUX5_9PEZI (GPI-anchored wall transfer protein n=5 Tax=Cercospora TaxID=29002 RepID=A0A2S6BUX5_9PEZI)

HSP 1 Score: 108 bits (271), Expect = 4.600e-24
Identity = 61/121 (50.41%), Postives = 72/121 (59.50%), Query Frame = 1
Query:  289 PAMSLFRGGLLLGTALGILAVDFPAFPRFYAKTEDYGASLMDIGVGGFVFAAGFSAQRPSFTEN--------------LFHSLPVLGLGFARWLSVWLSDYHTHTSEYGRDLNFFFVLAFL 609
            P ++ +RG +++ T   ILAVDFP FPR +AKTE +G SLMD+GVG FVFAAG  A R    E+              L HSLP+  LGF R LSV   DY  H SEYG   NFFF LA L
Sbjct:  132 PFITAYRGAMMIITCSSILAVDFPIFPRRFAKTESFGTSLMDMGVGSFVFAAGIVAARQQLKEDNEASRSLVKRLKSALRHSLPLAVLGFVRLLSVKKLDYAEHVSEYGVHWNFFFTLALL 252          
BLAST of mRNA_M-pyrifera_M_contig90217.20920.1 vs. uniprot
Match: N1JNF8_BLUG1 (GPI-anchored wall transfer protein n=4 Tax=Blumeria TaxID=34372 RepID=N1JNF8_BLUG1)

HSP 1 Score: 108 bits (270), Expect = 5.350e-24
Identity = 79/217 (36.41%), Postives = 111/217 (51.15%), Query Frame = 1
Query:    1 LLLLRGGFDAKPETWSKGFRLLLEAVVIVLPT-LFVFTIGHSMVYEVVMFQTVAIFVLGLTPLGQAGPVFHFDRLFDSTNAVAVINRNKKGTFRLRSPAMSLFRGGLLLGTALGILAVDFPAFPRFYAKTEDYGASLMDIGVGGFVFAAGFSA---------QRPSFTENLF----HSLPVLGLGFARWLSVWLSDYHTHTSEYGRDLNFFFVLAFL 609
            +L  R G       W+     L+  V I+L T L+ F   + ++  +++   V +  L L PL QA    H   L  +     V++ +       + P ++++RG +L+ TAL ILAVDF  FPR +AK E++G SLMD+GVG FVF+AG  A         Q+   +  L+    HSLP+L LG  R  SV   DY  H SEYG   NFFF L  L
Sbjct:   48 MLQSRHGLFHSSNVWTVTTDFLINVVAILLATTLYAF---NPLLLSILLLAPVCLLSL-LRPLAQARKKAHVKSLKRAALQHEVVSPSDPRYRLPKRPFLTMYRGSMLVITALSILAVDFRIFPRRFAKVENWGTSLMDVGVGSFVFSAGVVATRSIILSDGQQAGLSRRLWKSGRHSLPLLVLGLLRLWSVKELDYAEHVSEYGVHWNFFFTLGLL 260          
BLAST of mRNA_M-pyrifera_M_contig90217.20920.1 vs. uniprot
Match: A0A7D8YPF3_9HELO (GPI-anchored wall transfer protein n=5 Tax=Lachnellula TaxID=47830 RepID=A0A7D8YPF3_9HELO)

HSP 1 Score: 107 bits (268), Expect = 9.540e-24
Identity = 59/124 (47.58%), Postives = 75/124 (60.48%), Query Frame = 1
Query:  283 RSPAMSLFRGGLLLGTALGILAVDFPAFPRFYAKTEDYGASLMDIGVGGFVFAAGFSAQRPSFTE---------------NLFHSLPVLGLGFARWLSVWLSDYHTHTSEYGRDLNFFFVLAFL 609
            + P ++++RG +L+ T L ILAVDF  FPR +AK E++G SLMD+GVG FVF+AG  A RP   E               ++ HSLP+L LGF R  SV   DY  H +EYG   NFFF L FL
Sbjct:  130 KKPFLTIYRGAMLVVTCLAILAVDFRIFPRRFAKVENWGTSLMDVGVGSFVFSAGVVAARPILKEQSAGKSTALSTRLYKSIRHSLPLLVLGFIRLYSVKGLDYAEHVTEYGVHWNFFFTLGFL 253          
BLAST of mRNA_M-pyrifera_M_contig90217.20920.1 vs. uniprot
Match: A0A194W2H0_9PEZI (GPI-anchored wall transfer protein n=2 Tax=Valsaceae TaxID=5117 RepID=A0A194W2H0_9PEZI)

HSP 1 Score: 107 bits (268), Expect = 9.650e-24
Identity = 62/122 (50.82%), Postives = 73/122 (59.84%), Query Frame = 1
Query:  289 PAMSLFRGGLLLGTALGILAVDFPAFPRFYAKTEDYGASLMDIGVGGFVFAAGFSAQRPSFTE---------------NLFHSLPVLGLGFARWLSVWLSDYHTHTSEYGRDLNFFFVLAFL 609
            P +++FRG +L+ T L ILAVDF  FPR +AK E +G SLMDIGVG FVF+AG  A RP   E               ++ HSLP+L LG  R LSV   DY  H SEYG   NFFF L FL
Sbjct:  133 PFLTVFRGSMLVITCLAILAVDFRIFPRRFAKVETWGTSLMDIGVGAFVFSAGVVAARPVLKERAEGRTTPLLRRLVYSMRHSLPLLVLGVVRLLSVKGLDYAEHVSEYGVHWNFFFTLGFL 254          
BLAST of mRNA_M-pyrifera_M_contig90217.20920.1 vs. uniprot
Match: A0A3N2Q007_9PEZI (GPI-anchored wall transfer protein 1 n=1 Tax=Sodiomyces alkalinus F11 TaxID=1314773 RepID=A0A3N2Q007_9PEZI)

HSP 1 Score: 108 bits (269), Expect = 1.110e-23
Identity = 61/122 (50.00%), Postives = 73/122 (59.84%), Query Frame = 1
Query:  289 PAMSLFRGGLLLGTALGILAVDFPAFPRFYAKTEDYGASLMDIGVGGFVFAAGFSAQRPSFTE---------------NLFHSLPVLGLGFARWLSVWLSDYHTHTSEYGRDLNFFFVLAFL 609
            P ++ +RGG+L+ T + ILAVDF  FPR +AK E +G SLMD+GVG FVF+AG  A RP   E               +L HSLP+L LGF R LSV   DY  H SEYG   NFFF L  L
Sbjct:  142 PFLTNYRGGMLVITTIAILAVDFRLFPRRFAKVETWGTSLMDLGVGSFVFSAGVIAARPVLRERASGRSASLATRLRASLRHSLPLLALGFVRLLSVKGLDYAEHVSEYGVHWNFFFTLGLL 263          
BLAST of mRNA_M-pyrifera_M_contig90217.20920.1 vs. uniprot
Match: N1PRQ8_DOTSN (GPI-anchored wall transfer protein n=2 Tax=Mycosphaerellaceae TaxID=93133 RepID=N1PRQ8_DOTSN)

HSP 1 Score: 107 bits (268), Expect = 1.190e-23
Identity = 63/122 (51.64%), Postives = 75/122 (61.48%), Query Frame = 1
Query:  289 PAMSLFRGGLLLGTALGILAVDFPA-FPRFYAKTEDYGASLMDIGVGGFVFAAGFSAQRPSFTEN------LF--------HSLPVLGLGFARWLSVWLSDYHTHTSEYGRDLNFFFVLAFL 609
            P ++++RG +++ T   ILAVDFPA FPR +AKTE +G SLMD+GVG FVFAAG  + RP   EN      LF        HSLP+  LG  R LSV   DY  H SEYG   NFFF LA L
Sbjct:  136 PFVTMYRGAMMIVTCACILAVDFPAVFPRRFAKTESFGTSLMDMGVGSFVFAAGVISARPQLKENHAGSRSLFSRLTTAMRHSLPLFVLGIGRLLSVKSLDYAEHVSEYGVHWNFFFTLALL 257          
BLAST of mRNA_M-pyrifera_M_contig90217.20920.1 vs. uniprot
Match: A0A6G1KZQ8_9PEZI (GPI-anchored wall transfer protein n=1 Tax=Teratosphaeria nubilosa TaxID=161662 RepID=A0A6G1KZQ8_9PEZI)

HSP 1 Score: 107 bits (267), Expect = 1.670e-23
Identity = 63/130 (48.46%), Postives = 78/130 (60.00%), Query Frame = 1
Query:  265 KGTFRLRSPAMSLFRGGLLLGTALGILAVDFPAFPRFYAKTEDYGASLMDIGVGGFVFAAGFSAQRPS-----------FTENLFH----SLPVLGLGFARWLSVWLSDYHTHTSEYGRDLNFFFVLAFL 609
            KG+  ++ P ++ +RG +++ T   ILAVDFP FPR +AKTE +G SLMD+GVG FVFAAG +A RP            F E +      SLP+L LGF R  SV   DY  H SEYG   NFFF LA L
Sbjct:  137 KGSLPVK-PFITAYRGAMMIITCTSILAVDFPVFPRRFAKTEAFGTSLMDLGVGSFVFAAGIAAARPELKARAQNLNSPFVERMKSAIRPSLPLLALGFVRLWSVKGLDYAEHVSEYGVHWNFFFTLALL 265          
BLAST of mRNA_M-pyrifera_M_contig90217.20920.1 vs. uniprot
Match: A0A553HNY9_9PEZI (GPI-anchored wall transfer protein 1 n=1 Tax=Xylaria flabelliformis TaxID=2512241 RepID=A0A553HNY9_9PEZI)

HSP 1 Score: 107 bits (268), Expect = 1.760e-23
Identity = 60/122 (49.18%), Postives = 74/122 (60.66%), Query Frame = 1
Query:  289 PAMSLFRGGLLLGTALGILAVDFPAFPRFYAKTEDYGASLMDIGVGGFVFAAGFSAQRPSFTE---------------NLFHSLPVLGLGFARWLSVWLSDYHTHTSEYGRDLNFFFVLAFL 609
            P ++ +RG LL+ T LGILAVDF  FPR +AK E +G S+MD+GVG FVF+AG  A RP   E               ++ HSLP+L LG AR +SV   DY  H +EYG   NFFF L FL
Sbjct:  719 PFLTTYRGSLLVLTCLGILAVDFRIFPRRFAKVETWGTSIMDLGVGSFVFSAGIVAARPLLKERAEGRATPLSQRLIYSMRHSLPLLVLGIARLISVKGLDYAEHVTEYGVHWNFFFTLGFL 840          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig90217.20920.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7I8VS01_9ANNE1.230e-3036.32DgyrCDS7167 n=1 Tax=Dimorphilus gyrociliatus TaxID... [more]
A0A218ZD83_9HELO2.850e-2442.31GPI-anchored wall transfer protein n=1 Tax=Marsson... [more]
A0A2S6BUX5_9PEZI4.600e-2450.41GPI-anchored wall transfer protein n=5 Tax=Cercosp... [more]
N1JNF8_BLUG15.350e-2436.41GPI-anchored wall transfer protein n=4 Tax=Blumeri... [more]
A0A7D8YPF3_9HELO9.540e-2447.58GPI-anchored wall transfer protein n=5 Tax=Lachnel... [more]
A0A194W2H0_9PEZI9.650e-2450.82GPI-anchored wall transfer protein n=2 Tax=Valsace... [more]
A0A3N2Q007_9PEZI1.110e-2350.00GPI-anchored wall transfer protein 1 n=1 Tax=Sodio... [more]
N1PRQ8_DOTSN1.190e-2351.64GPI-anchored wall transfer protein n=2 Tax=Mycosph... [more]
A0A6G1KZQ8_9PEZI1.670e-2348.46GPI-anchored wall transfer protein n=1 Tax=Teratos... [more]
A0A553HNY9_9PEZI1.760e-2349.18GPI-anchored wall transfer protein 1 n=1 Tax=Xylar... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig90217contigM-pyrifera_M_contig90217:142..762 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score110.2
Seed ortholog evalue1.2e-21
Seed eggNOG ortholog64363.EME46131
Preferred nameGWT1
KEGG rclassRC00004
KEGG koko:K05283
KEGG ReactionR05918
KEGG Pathwayko00563,ko01100,map00563,map01100
KEGG ModuleM00065
Hectar predicted targeting categorysignal anchor
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0006464,GO:0006497,GO:0006505,GO:0006506,GO:0006629,GO:0006643,GO:0006644,GO:0006650,GO:0006661,GO:0006664,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0008374,GO:0008610,GO:0008654,GO:0009058,GO:0009059,GO:0009247,GO:0009987,GO:0012505,GO:0016020,GO:0016021,GO:0016740,GO:0016746,GO:0016747,GO:0019538,GO:0019637,GO:0031224,GO:0031984,GO:0032216,GO:0034645,GO:0035690,GO:0036211,GO:0042157,GO:0042158,GO:0042175,GO:0042221,GO:0042493,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0045017,GO:0046467,GO:0046474,GO:0046486,GO:0046488,GO:0050896,GO:0051716,GO:0070887,GO:0071704,GO:0090407,GO:0098827,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576,GO:1903509
EggNOG free text desc.acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI- anchor
EggNOG OGs1ZYCG@147541,38CY0@33154,3MHAG@451867,3NUJT@4751,3QJD8@4890,COG5062@1,KOG0411@2759
Ec32 ortholog descriptionGWT1
Ec32 orthologEc-01_003370.2
COG Functional cat.I
Best tax levelDothideomycetidae
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons1
Model size621
Cds size621
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816813.9825175-CDS-M-pyrifera_M_contig90217:141..7621622816813.9825175-CDS-M-pyrifera_M_contig90217:141..762Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90217 142..762 +
1692278193.9764407-CDS-M-pyrifera_M_contig90217:141..7621692278193.9764407-CDS-M-pyrifera_M_contig90217:141..762Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90217 142..762 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig90217.20920.1prot_M-pyrifera_M_contig90217.20920.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig90217 142..762 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig90217.20920.1

>prot_M-pyrifera_M_contig90217.20920.1 ID=prot_M-pyrifera_M_contig90217.20920.1|Name=mRNA_M-pyrifera_M_contig90217.20920.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=207bp
LLLLRGGFDAKPETWSKGFRLLLEAVVIVLPTLFVFTIGHSMVYEVVMFQ
TVAIFVLGLTPLGQAGPVFHFDRLFDSTNAVAVINRNKKGTFRLRSPAMS
LFRGGLLLGTALGILAVDFPAFPRFYAKTEDYGASLMDIGVGGFVFAAGF
SAQRPSFTENLFHSLPVLGLGFARWLSVWLSDYHTHTSEYGRDLNFFFVL
AFLQLGA
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mRNA from alignment at M-pyrifera_M_contig90217:142..762+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig90217.20920.1 ID=mRNA_M-pyrifera_M_contig90217.20920.1|Name=mRNA_M-pyrifera_M_contig90217.20920.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=621bp|location=Sequence derived from alignment at M-pyrifera_M_contig90217:142..762+ (Macrocystis pyrifera P11B4 male)
CTCTTGTTGTTACGTGGTGGGTTTGACGCAAAGCCAGAAACATGGAGCAA AGGGTTTCGTTTGCTCCTCGAGGCCGTGGTAATCGTGCTGCCTACGCTTT TTGTGTTCACAATTGGACATTCTATGGTGTATGAGGTAGTGATGTTTCAA ACCGTTGCAATTTTTGTCTTGGGGTTGACTCCGTTGGGGCAAGCAGGGCC AGTTTTTCATTTCGACCGCCTGTTTGACTCAACAAATGCTGTTGCGGTGA TAAATCGCAATAAAAAAGGTACCTTCCGTCTTCGTAGTCCCGCCATGTCA CTCTTCCGTGGAGGACTGTTGCTTGGAACAGCTTTAGGGATTTTGGCAGT CGATTTCCCTGCATTTCCTCGATTCTATGCGAAAACAGAAGACTATGGTG CGAGTTTGATGGACATTGGGGTTGGTGGATTTGTGTTTGCTGCTGGATTT TCCGCACAACGCCCATCGTTCACCGAAAACCTCTTCCATTCCCTCCCAGT CCTTGGTCTTGGTTTTGCAAGATGGCTGTCAGTTTGGTTGAGTGATTACC ACACCCACACAAGTGAATATGGTCGTGATTTGAACTTTTTCTTTGTCCTC GCTTTCCTTCAACTTGGAGCA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig90217:142..762+

>mRNA_M-pyrifera_M_contig90217.20920.1 ID=mRNA_M-pyrifera_M_contig90217.20920.1|Name=mRNA_M-pyrifera_M_contig90217.20920.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1242bp|location=Sequence derived from alignment at M-pyrifera_M_contig90217:142..762+ (Macrocystis pyrifera P11B4 male)
CTCTTGTTGTTACGTGGTGGGTTTGACGCAAAGCCAGAAACATGGAGCAA
AGGGTTTCGTTTGCTCCTCGAGGCCGTGGTAATCGTGCTGCCTACGCTTT
TTGTGTTCACAATTGGACATTCTATGGTGTATGAGGTAGTGATGTTTCAA
ACCGTTGCAATTTTTGTCTTGGGGTTGACTCCGTTGGGGCAAGCAGGGCC
AGTTTTTCATTTCGACCGCCTGTTTGACTCAACAAATGCTGTTGCGGTGA
TAAATCGCAATAAAAAAGGTACCTTCCGTCTTCGTAGTCCCGCCATGTCA
CTCTTCCGTGGAGGACTGTTGCTTGGAACAGCTTTAGGGATTTTGGCAGT
CGATTTCCCTGCATTTCCTCGATTCTATGCGAAAACAGAAGACTATGGTG
CGAGTTTGATGGACATTGGGGTTGGTGGATTTGTGTTTGCTGCTGGATTT
TCCGCACAACGCCCATCGTTCACCGAAAACCTCTTCCATTCCCTCCCAGT
CCTTGGTCTTGGTTTTGCAAGATGGCTGTCAGTTTGGTTGAGTGATTACC
ACACCCACACAAGTGAATATGGTCGTGATTTGAACTTTTTCTTTGTCCTC
GCTTTCCTTCAACTTGGAGCACTCTTGTTGTTACGTGGTGGGTTTGACGC
AAAGCCAGAAACATGGAGCAAAGGGTTTCGTTTGCTCCTCGAGGCCGTGG
TAATCGTGCTGCCTACGCTTTTTGTGTTCACAATTGGACATTCTATGGTG
TATGAGGTAGTGATGTTTCAAACCGTTGCAATTTTTGTCTTGGGGTTGAC
TCCGTTGGGGCAAGCAGGGCCAGTTTTTCATTTCGACCGCCTGTTTGACT
CAACAAATGCTGTTGCGGTGATAAATCGCAATAAAAAAGGTACCTTCCGT
CTTCGTAGTCCCGCCATGTCACTCTTCCGTGGAGGACTGTTGCTTGGAAC
AGCTTTAGGGATTTTGGCAGTCGATTTCCCTGCATTTCCTCGATTCTATG
CGAAAACAGAAGACTATGGTGCGAGTTTGATGGACATTGGGGTTGGTGGA
TTTGTGTTTGCTGCTGGATTTTCCGCACAACGCCCATCGTTCACCGAAAA
CCTCTTCCATTCCCTCCCAGTCCTTGGTCTTGGTTTTGCAAGATGGCTGT
CAGTTTGGTTGAGTGATTACCACACCCACACAAGTGAATATGGTCGTGAT
TTGAACTTTTTCTTTGTCCTCGCTTTCCTTCAACTTGGAGCA
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