mRNA_M-pyrifera_M_contig89815.20819.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig89815.20819.1
Unique NamemRNA_M-pyrifera_M_contig89815.20819.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig89815.20819.1 vs. uniprot
Match: W4FLE1_9STRA (Uncharacterized protein n=8 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FLE1_9STRA)

HSP 1 Score: 117 bits (292), Expect = 1.240e-28
Identity = 62/113 (54.87%), Postives = 74/113 (65.49%), Query Frame = 1
Query:   13 WGPGDGRPVEELKKAVSDLTDEYLLSGDLDEAVRCVVELESPSFHHEVVKRVCVRSVERRDPGTEAGAALLARLYERGLVTNVQLQHGLKRVVETLEDLSLDVPDTSVLLASL 351
            WGPGDGRPVEELK A+  LT EYLLSGDL+EA  CV EL  P FHHEVVKR  + S E     T A  +LLA L  + +V+  QL+ G+ +V  TL D+ LDVP    LL +L
Sbjct:  296 WGPGDGRPVEELKVAIDQLTKEYLLSGDLEEASACVRELNVPHFHHEVVKRGIINSFEEGKEYTNAIGSLLAYLVSQDIVSTAQLRKGVDKVALTLPDIQLDVPGAKGLLTAL 408          
BLAST of mRNA_M-pyrifera_M_contig89815.20819.1 vs. uniprot
Match: A0A024UPC0_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UPC0_9STRA)

HSP 1 Score: 115 bits (288), Expect = 4.370e-28
Identity = 61/113 (53.98%), Postives = 74/113 (65.49%), Query Frame = 1
Query:   13 WGPGDGRPVEELKKAVSDLTDEYLLSGDLDEAVRCVVELESPSFHHEVVKRVCVRSVERRDPGTEAGAALLARLYERGLVTNVQLQHGLKRVVETLEDLSLDVPDTSVLLASL 351
            WGPGDGRPVEELK A+  LT EYLLSGDL+EA  CV EL  P FHHEVVKR  + S E     T A  +LLA L  + +V+  QL+ G+ +V   L D+ LDVP    LL++L
Sbjct:  291 WGPGDGRPVEELKVAIDQLTKEYLLSGDLEEASACVRELNVPHFHHEVVKRGIINSFEEGKEYTNAIGSLLAYLVSQDIVSTAQLRKGVDKVALALPDIQLDVPGAKGLLSAL 403          
BLAST of mRNA_M-pyrifera_M_contig89815.20819.1 vs. uniprot
Match: A0A6G0XG25_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XG25_9STRA)

HSP 1 Score: 112 bits (280), Expect = 6.710e-27
Identity = 61/113 (53.98%), Postives = 72/113 (63.72%), Query Frame = 1
Query:   13 WGPGDGRPVEELKKAVSDLTDEYLLSGDLDEAVRCVVELESPSFHHEVVKRVCVRSVERRDPGTEAGAALLARLYERGLVTNVQLQHGLKRVVETLEDLSLDVPDTSVLLASL 351
            WGPGDGRPVEELK A+  LT EYLLSGDL+EA  CV EL    FHHEVVKR  + S E       A  +LLA L  + +V++ QL+ G  +V   L DL LDVP    LLA+L
Sbjct:  300 WGPGDGRPVEELKVAIDQLTKEYLLSGDLEEAATCVRELNVAHFHHEVVKRGIINSFEEGKEYCNAMGSLLAFLVSQEIVSSAQLRKGFDKVALVLPDLELDVPGARKLLAAL 412          
BLAST of mRNA_M-pyrifera_M_contig89815.20819.1 vs. uniprot
Match: A0A485L5H6_9STRA (Aste57867_15208 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485L5H6_9STRA)

HSP 1 Score: 110 bits (276), Expect = 2.690e-26
Identity = 59/113 (52.21%), Postives = 73/113 (64.60%), Query Frame = 1
Query:   13 WGPGDGRPVEELKKAVSDLTDEYLLSGDLDEAVRCVVELESPSFHHEVVKRVCVRSVERRDPGTEAGAALLARLYERGLVTNVQLQHGLKRVVETLEDLSLDVPDTSVLLASL 351
            WGPGDGRPVEELK A+  LT EYLLSGD++EA  CV EL    FHHEVVKR  + S E     + A  +LLA L  + +V++ QL+ G  +V   + DL LDVP    LLA+L
Sbjct:  307 WGPGDGRPVEELKVAIDQLTKEYLLSGDVNEAATCVRELNVAHFHHEVVKRGIINSFEEGKEYSNAMGSLLAYLVSQEIVSSAQLRKGFDKVALAIPDLQLDVPAAPKLLAAL 419          
BLAST of mRNA_M-pyrifera_M_contig89815.20819.1 vs. uniprot
Match: A0A0W8DUN2_PHYNI (tRNA 2'-phosphotransferase 1 n=1 Tax=Phytophthora nicotianae TaxID=4790 RepID=A0A0W8DUN2_PHYNI)

HSP 1 Score: 108 bits (269), Expect = 4.210e-26
Identity = 58/112 (51.79%), Postives = 72/112 (64.29%), Query Frame = 1
Query:    7 QIWGPGDGRPVEELKKAVSDLTDEYLLSGDLDEAVRCVVELESPSFHHEVVKRVCVRSVERRDPGTEAG-AALLARLYERGLVTNVQLQHGLKRVVETLEDLSLDVPDTSVL 339
            + WGPGDGRPVEELK A+  LT EYLLS DL+EA RCV EL  P FHHEVVKR    S+E       A  A+LLA L    +V+  QL  G +R    L+D++LD+P+ + L
Sbjct:  163 KAWGPGDGRPVEELKVAIDQLTKEYLLSRDLEEAARCVRELNVPHFHHEVVKRGITNSLEEGGEANSAAMASLLAYLVSNEVVSTGQLIKGFERFKFVLDDVALDIPNAAAL 274          
BLAST of mRNA_M-pyrifera_M_contig89815.20819.1 vs. uniprot
Match: A0A484EHM1_BRELC (Uncharacterized protein n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484EHM1_BRELC)

HSP 1 Score: 109 bits (272), Expect = 7.450e-26
Identity = 60/112 (53.57%), Postives = 72/112 (64.29%), Query Frame = 1
Query:    7 QIWGPGDGRPVEELKKAVSDLTDEYLLSGDLDEAVRCVVELESPSFHHEVVKRVCVRSVERRDPGTEAG-AALLARLYERGLVTNVQLQHGLKRVVETLEDLSLDVPDTSVL 339
            + WGPGDGRPVEELK A+  LT EYLLS DLDEA RCV EL  P FHHEVVKR  + S+E       A  A+LLA L    +V+  QL  G +R    L D++LD+P+ S L
Sbjct:  271 KAWGPGDGRPVEELKVAIDQLTKEYLLSRDLDEAARCVRELNVPHFHHEVVKRGLMNSLEEGGEANGAAMASLLAYLVSNEVVSTGQLVKGFQRFQFVLNDVALDIPNASKL 382          
BLAST of mRNA_M-pyrifera_M_contig89815.20819.1 vs. uniprot
Match: A0A0P1AHZ2_PLAHL (Ma3 domain-containing protein isoform 1 n=2 Tax=Peronosporaceae TaxID=4777 RepID=A0A0P1AHZ2_PLAHL)

HSP 1 Score: 108 bits (270), Expect = 1.330e-25
Identity = 59/112 (52.68%), Postives = 72/112 (64.29%), Query Frame = 1
Query:    7 QIWGPGDGRPVEELKKAVSDLTDEYLLSGDLDEAVRCVVELESPSFHHEVVKRVCVRSVERRDPGTEAG-AALLARLYERGLVTNVQLQHGLKRVVETLEDLSLDVPDTSVL 339
            + WGPGDGRPVEELK A+  LT EYLLS DLDEA RCV EL  P FHHEVVKR    S+E       A  A+LLA L    +V+  QL  G +R    L+D++LD+P+ + L
Sbjct:  263 KAWGPGDGRPVEELKVAIDQLTKEYLLSRDLDEAARCVRELNVPHFHHEVVKRGINNSLEEGGEANSAAMASLLAYLVSNEVVSTGQLIKGFERFKFVLDDVALDIPNAAAL 374          
BLAST of mRNA_M-pyrifera_M_contig89815.20819.1 vs. uniprot
Match: D0NNQ0_PHYIT (Programmed cell death protein, putative n=11 Tax=Phytophthora TaxID=4783 RepID=D0NNQ0_PHYIT)

HSP 1 Score: 108 bits (270), Expect = 1.470e-25
Identity = 58/112 (51.79%), Postives = 72/112 (64.29%), Query Frame = 1
Query:    7 QIWGPGDGRPVEELKKAVSDLTDEYLLSGDLDEAVRCVVELESPSFHHEVVKRVCVRSVERRDPGTEAG-AALLARLYERGLVTNVQLQHGLKRVVETLEDLSLDVPDTSVL 339
            + WGPGDGRPVEELK A+  LT EYLLS DL+EA RCV EL  P FHHEVVKR    S+E       A  A+LLA L    +V+  QL  G +R    L+D++LD+P+ + L
Sbjct:  272 KAWGPGDGRPVEELKVAIDQLTKEYLLSRDLEEAARCVRELNVPHFHHEVVKRGITNSLEEGGEANSAAMASLLAYLVSNEVVSTGQLVKGFERFKFVLDDVALDIPNAAAL 383          
BLAST of mRNA_M-pyrifera_M_contig89815.20819.1 vs. uniprot
Match: A0A5D6XR47_9STRA (Uncharacterized protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XR47_9STRA)

HSP 1 Score: 108 bits (269), Expect = 1.870e-25
Identity = 58/110 (52.73%), Postives = 72/110 (65.45%), Query Frame = 1
Query:    7 QIWGPGDGRPVEELKKAVSDLTDEYLLSGDLDEAVRCVVELESPSFHHEVVKRVCVRSVERRDPGTE-AGAALLARLYERGLVTNVQLQHGLKRVVETLEDLSLDVPDTS 333
            + WGPGDGRPVEELK A+  LT EYLLS DL+EA RCV EL  P FHHEVVKR    S+E     T  A ++LLA L  + +V+  QL+ G  R    L D++LD+P+ S
Sbjct:  265 KAWGPGDGRPVEELKVAIDQLTKEYLLSSDLEEAARCVRELNVPHFHHEVVKRGITNSLEEGGDATPLAMSSLLAYLVAQEIVSPDQLRKGFARFKMVLSDVALDIPNAS 374          
BLAST of mRNA_M-pyrifera_M_contig89815.20819.1 vs. uniprot
Match: A0A7S1D6M5_CYCTE (Hypothetical protein n=1 Tax=Cyclophora tenuis TaxID=216820 RepID=A0A7S1D6M5_CYCTE)

HSP 1 Score: 108 bits (270), Expect = 1.950e-25
Identity = 57/114 (50.00%), Postives = 72/114 (63.16%), Query Frame = 1
Query:    7 QIWGPGDGRPVEELKKAVSDLTDEYLLSGDLDEAVRCVVELESPSFHHEVVKRVCVRSVERRDPGT--EAGAALLARLYERGLVTNVQLQHGLKRVVETLEDLSLDVPDTSVLL 342
            ++WGPGDGRPV ELK  +  L  EYLLS +LDEA RCV EL SP FHHE+VKR    ++E   P    +A AAL   L +  +V+  Q+  G+ R+ + L DLSLDVP    LL
Sbjct:  305 RVWGPGDGRPVSELKAVLDQLLKEYLLSRELDEAARCVRELGSPHFHHELVKRGVTHAMEEASPSESLDAMAALFEFLVKNAIVSEYQVAKGISRLQKLLPDLSLDVPSAPTLL 418          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig89815.20819.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
W4FLE1_9STRA1.240e-2854.87Uncharacterized protein n=8 Tax=Aphanomyces astaci... [more]
A0A024UPC0_9STRA4.370e-2853.98Uncharacterized protein n=1 Tax=Aphanomyces invada... [more]
A0A6G0XG25_9STRA6.710e-2753.98Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
A0A485L5H6_9STRA2.690e-2652.21Aste57867_15208 protein n=1 Tax=Aphanomyces stella... [more]
A0A0W8DUN2_PHYNI4.210e-2651.79tRNA 2'-phosphotransferase 1 n=1 Tax=Phytophthora ... [more]
A0A484EHM1_BRELC7.450e-2653.57Uncharacterized protein n=1 Tax=Bremia lactucae Ta... [more]
A0A0P1AHZ2_PLAHL1.330e-2552.68Ma3 domain-containing protein isoform 1 n=2 Tax=Pe... [more]
D0NNQ0_PHYIT1.470e-2551.79Programmed cell death protein, putative n=11 Tax=P... [more]
A0A5D6XR47_9STRA1.870e-2552.73Uncharacterized protein n=1 Tax=Pythium brassicum ... [more]
A0A7S1D6M5_CYCTE1.950e-2550.00Hypothetical protein n=1 Tax=Cyclophora tenuis Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig89815contigM-pyrifera_M_contig89815:175..675 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score116.3
Seed ortholog evalue1e-23
Seed eggNOG ortholog157072.XP_008862101.1
Preferred namePDCD4
KEGG koko:K01228,ko:K16865
KEGG ReactionR05979
KEGG Pathwayko00510,ko01100,ko04141,ko05205,ko05206,map00510,map01100,map04141,map05205,map05206
KEGG ModuleM00073
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0001817,GO:0001818,GO:0001837,GO:0001932,GO:0001933,GO:0002237,GO:0003006,GO:0003007,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005829,GO:0006355,GO:0006357,GO:0006469,GO:0006915,GO:0007154,GO:0007165,GO:0007166,GO:0007167,GO:0007178,GO:0007275,GO:0007276,GO:0007281,GO:0007292,GO:0007293,GO:0007507,GO:0007568,GO:0007569,GO:0008150,GO:0008219,GO:0008283,GO:0008285,GO:0009605,GO:0009607,GO:0009617,GO:0009653,GO:0009719,GO:0009887,GO:0009888,GO:0009889,GO:0009890,GO:0009892,GO:0009966,GO:0009967,GO:0009968,GO:0009987,GO:0010033,GO:0010468,GO:0010556,GO:0010558,GO:0010563,GO:0010605,GO:0010629,GO:0010646,GO:0010647,GO:0010648,GO:0010660,GO:0010661,GO:0010941,GO:0010942,GO:0012501,GO:0019219,GO:0019220,GO:0019221,GO:0019222,GO:0019953,GO:0022412,GO:0022414,GO:0023051,GO:0023052,GO:0023056,GO:0023057,GO:0030154,GO:0030509,GO:0030727,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031347,GO:0031348,GO:0031349,GO:0031399,GO:0031400,GO:0032101,GO:0032102,GO:0032103,GO:0032268,GO:0032269,GO:0032496,GO:0032501,GO:0032502,GO:0032504,GO:0032872,GO:0032873,GO:0033673,GO:0033993,GO:0034097,GO:0034391,GO:0034393,GO:0035051,GO:0035722,GO:0042127,GO:0042221,GO:0042325,GO:0042326,GO:0042981,GO:0043065,GO:0043066,GO:0043067,GO:0043068,GO:0043069,GO:0043086,GO:0043207,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043405,GO:0043407,GO:0043408,GO:0043409,GO:0043506,GO:0043508,GO:0043549,GO:0044092,GO:0044424,GO:0044444,GO:0044464,GO:0044703,GO:0045595,GO:0045596,GO:0045597,GO:0045786,GO:0045859,GO:0045892,GO:0045934,GO:0045936,GO:0046328,GO:0046329,GO:0048134,GO:0048135,GO:0048468,GO:0048477,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0048585,GO:0048609,GO:0048646,GO:0048660,GO:0048662,GO:0048731,GO:0048762,GO:0048856,GO:0048869,GO:0050727,GO:0050728,GO:0050729,GO:0050789,GO:0050790,GO:0050793,GO:0050794,GO:0050896,GO:0051093,GO:0051094,GO:0051147,GO:0051148,GO:0051150,GO:0051151,GO:0051171,GO:0051172,GO:0051174,GO:0051239,GO:0051241,GO:0051246,GO:0051248,GO:0051252,GO:0051253,GO:0051338,GO:0051348,GO:0051704,GO:0051707,GO:0051716,GO:0051726,GO:0055006,GO:0060255,GO:0060317,GO:0060485,GO:0060548,GO:0060935,GO:0060936,GO:0060938,GO:0060939,GO:0060940,GO:0065007,GO:0065009,GO:0070302,GO:0070303,GO:0070671,GO:0070848,GO:0070887,GO:0071216,GO:0071219,GO:0071222,GO:0071310,GO:0071345,GO:0071349,GO:0071363,GO:0071396,GO:0071495,GO:0071772,GO:0071773,GO:0071900,GO:0071901,GO:0072359,GO:0080090,GO:0080134,GO:0080135,GO:1900015,GO:1900016,GO:1901222,GO:1901224,GO:1901342,GO:1901343,GO:1901700,GO:1901701,GO:1902531,GO:1902532,GO:1902533,GO:1902679,GO:1903506,GO:1903507,GO:1904035,GO:1904037,GO:1904705,GO:1904706,GO:1904760,GO:1904761,GO:1905063,GO:1905064,GO:1905459,GO:1905461,GO:2000026,GO:2000112,GO:2000113,GO:2000351,GO:2000353,GO:2000736,GO:2000738,GO:2001141
EggNOG free text desc.epithelial to mesenchymal transition involved in cardiac fibroblast development
EggNOG OGsKOG0403@1,KOG0403@2759
EC3.2.1.106
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko04147
Exons3
Model size369
Cds size369
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig89815.20819.1prot_M-pyrifera_M_contig89815.20819.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig89815 175..675 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816809.0438778-CDS-M-pyrifera_M_contig89815:174..2491622816809.0438778-CDS-M-pyrifera_M_contig89815:174..249Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89815 175..249 -
1692278191.1688511-CDS-M-pyrifera_M_contig89815:174..2491692278191.1688511-CDS-M-pyrifera_M_contig89815:174..249Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89815 175..249 -
1622816809.054247-CDS-M-pyrifera_M_contig89815:319..5211622816809.054247-CDS-M-pyrifera_M_contig89815:319..521Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89815 320..521 -
1692278191.1860476-CDS-M-pyrifera_M_contig89815:319..5211692278191.1860476-CDS-M-pyrifera_M_contig89815:319..521Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89815 320..521 -
1622816809.0660822-CDS-M-pyrifera_M_contig89815:583..6751622816809.0660822-CDS-M-pyrifera_M_contig89815:583..675Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89815 584..675 -
1692278191.1967907-CDS-M-pyrifera_M_contig89815:583..6751692278191.1967907-CDS-M-pyrifera_M_contig89815:583..675Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89815 584..675 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig89815.20819.1

>prot_M-pyrifera_M_contig89815.20819.1 ID=prot_M-pyrifera_M_contig89815.20819.1|Name=mRNA_M-pyrifera_M_contig89815.20819.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=123bp
VAQIWGPGDGRPVEELKKAVSDLTDEYLLSGDLDEAVRCVVELESPSFHH
EVVKRVCVRSVERRDPGTEAGAALLARLYERGLVTNVQLQHGLKRVVETL
EDLSLDVPDTSVLLASLLISLSN
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mRNA from alignment at M-pyrifera_M_contig89815:175..675-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig89815.20819.1 ID=mRNA_M-pyrifera_M_contig89815.20819.1|Name=mRNA_M-pyrifera_M_contig89815.20819.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=501bp|location=Sequence derived from alignment at M-pyrifera_M_contig89815:175..675- (Macrocystis pyrifera P11B4 male)
GTTGCGCAGATCTGGGGACCCGGCGATGGGCGCCCCGTGGAGGAGTTGAA GAAGGCGGTGTCTGACCTCACGGACGAGTACCTCCTGTCTGGGTGCGTCT GTTTGCGAGCGTGTGTACGGGCGCGTGCACGTACACACACACACACACAC ACAGCGATCTGGATGAAGCTGTGCGCTGCGTTGTGGAGCTGGAATCCCCC AGCTTCCACCACGAGGTCGTGAAGCGGGTGTGTGTGCGCAGCGTGGAGCG TCGTGACCCTGGGACCGAAGCGGGAGCTGCGTTGCTGGCGAGGCTGTACG AGCGAGGGCTGGTGACGAACGTGCAGCTGCAGCATGGGCTGAAGCGAGTG GTGGAGGTGGGTGGTGTGTTGTGTGTGTGTGTGCGCTTTGGGGAGGGTGT GTGCATGACTCACCGTGTCGATGCAGACGCTGGAGGATCTGTCGCTGGAT GTGCCGGACACGTCTGTGCTGCTGGCCTCGCTGCTCATCTCGCTGTCAAA C
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig89815:175..675-

>mRNA_M-pyrifera_M_contig89815.20819.1 ID=mRNA_M-pyrifera_M_contig89815.20819.1|Name=mRNA_M-pyrifera_M_contig89815.20819.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=738bp|location=Sequence derived from alignment at M-pyrifera_M_contig89815:175..675- (Macrocystis pyrifera P11B4 male)
GTTGCGCAGATCTGGGGACCCGGCGATGGGCGCCCCGTGGAGGAGTTGAA
GAAGGCGGTGTCTGACCTCACGGACGAGTACCTCCTGTCTGGGTTGCGCA
GATCTGGGGACCCGGCGATGGGCGCCCCGTGGAGGAGTTGAAGAAGGCGG
TGTCTGACCTCACGGACGAGTACCTCCTGTCTGGCGATCTGGATGAAGCT
GTGCGCTGCGTTGTGGAGCTGGAATCCCCCAGCTTCCACCACGAGGTCGT
GAAGCGGGTGTGTGTGCGCAGCGTGGAGCGTCGTGACCCTGGGACCGAAG
CGGGAGCTGCGTTGCTGGCGAGGCTGTACGAGCGAGGGCTGGTGACGAAC
GTGCAGCTGCAGCATGGGCTGAAGCGAGTGGTGGAGCGATCTGGATGAAG
CTGTGCGCTGCGTTGTGGAGCTGGAATCCCCCAGCTTCCACCACGAGGTC
GTGAAGCGGGTGTGTGTGCGCAGCGTGGAGCGTCGTGACCCTGGGACCGA
AGCGGGAGCTGCGTTGCTGGCGAGGCTGTACGAGCGAGGGCTGGTGACGA
ACGTGCAGCTGCAGCATGGGCTGAAGCGAGTGGTGGAGACGCTGGAGGAT
CTGTCGCTGGATGTGCCGGACACGTCTGTGCTGCTGGCCTCGCTGCTCAT
CTCGCTGTCAAACACGCTGGAGGATCTGTCGCTGGATGTGCCGGACACGT
CTGTGCTGCTGGCCTCGCTGCTCATCTCGCTGTCAAAC
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