mRNA_M-pyrifera_M_contig89473.20741.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig89473.20741.1
Unique NamemRNA_M-pyrifera_M_contig89473.20741.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig89473.20741.1 vs. uniprot
Match: A0A5A8BAF9_9PLAN (Glutamate synthase large subunit n=26 Tax=Planctomycetaceae TaxID=126 RepID=A0A5A8BAF9_9PLAN)

HSP 1 Score: 233 bits (593), Expect = 1.300e-68
Identity = 117/118 (99.15%), Postives = 118/118 (100.00%), Query Frame = 1 
Query:    1 ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLEDAEDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD 354
            ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLED+EDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD
Sbjct: 1384 ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLEDSEDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD 1501
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BLAST of mRNA_M-pyrifera_M_contig89473.20741.1 vs. uniprot
Match: A0A3D3RG18_9PLAN (Glutamate synthase subunit alpha (Fragment) n=1 Tax=Gimesia maris TaxID=122 RepID=A0A3D3RG18_9PLAN)

HSP 1 Score: 209 bits (532), Expect = 1.990e-62
Identity = 103/118 (87.29%), Postives = 112/118 (94.92%), Query Frame = 1 
Query:    1 ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLEDAEDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD 354
            ERFCVRNSGA AVVEGIGDHGCEYMTGGRAV+LGETGRNFAAGMSGGVAYVYDPEG LLQNSNLET+ELE +E+A+DIAELK LIDNHRKFTGST+AK ILD+WE+ELE FKKVMP+D
Sbjct:  476 ERFCVRNSGASAVVEGIGDHGCEYMTGGRAVILGETGRNFAAGMSGGVAYVYDPEGLLLQNSNLETIELEPVEEADDIAELKALIDNHRKFTGSTIAKKILDHWESELESFKKVMPID 593
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BLAST of mRNA_M-pyrifera_M_contig89473.20741.1 vs. uniprot
Match: A0A517SMN7_9PLAN (Ferredoxin-dependent glutamate synthase 1 n=1 Tax=Caulifigura coniformis TaxID=2527983 RepID=A0A517SMN7_9PLAN)

HSP 1 Score: 185 bits (469), Expect = 6.560e-52
Identity = 90/118 (76.27%), Postives = 104/118 (88.14%), Query Frame = 1 
Query:    1 ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLEDAEDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD 354
            ERFCVRNSGAKAVVEG GDHGCEYMTGGRAVVLG TGRNFAAGMSGGV+YV+DP G LL+N NLE VELE++E+ +D+AELKE+I NH+++TGS VA AIL+NW+ EL  F KVMPVD
Sbjct: 1428 ERFCVRNSGAKAVVEGCGDHGCEYMTGGRAVVLGATGRNFAAGMSGGVSYVWDPNGKLLENVNLEMVELEKIEEEDDVAELKEMISNHQRYTGSAVAGAILENWDEELPRFHKVMPVD 1545
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BLAST of mRNA_M-pyrifera_M_contig89473.20741.1 vs. uniprot
Match: A0A7C2PGV5_9PLAN (Glutamate synthase subunit alpha n=1 Tax=Schlesneria paludicola TaxID=360056 RepID=A0A7C2PGV5_9PLAN)

HSP 1 Score: 172 bits (436), Expect = 2.890e-50
Identity = 85/118 (72.03%), Postives = 97/118 (82.20%), Query Frame = 1 
Query:    1 ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLEDAEDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD 354
            ERFCVRNSGA  VVEG+GDHGCEYMTGGRAV+LG TGRNFAAGMSGG+AYV+DP   LL N NLE VELE +E  EDIAEL+ELI  H ++TGSTVA  IL +W+ EL  F+KVMP+D
Sbjct:  233 ERFCVRNSGAWTVVEGVGDHGCEYMTGGRAVILGPTGRNFAAGMSGGIAYVWDPSDQLLANCNLEMVELEPVEAGEDIAELRELIQKHERYTGSTVAADILKHWDRELPRFRKVMPID 350
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BLAST of mRNA_M-pyrifera_M_contig89473.20741.1 vs. uniprot
Match: UPI001420E1E3 (glutamate synthase large subunit n=1 Tax=Rubinisphaera sp. JC658 TaxID=2713176 RepID=UPI001420E1E3)

HSP 1 Score: 179 bits (455), Expect = 5.050e-50
Identity = 89/118 (75.42%), Postives = 100/118 (84.75%), Query Frame = 1 
Query:    1 ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLEDAEDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD 354
            ERFCVRNSGAKAVVEG+GDHGCEYMTGGRAV+LG TGRNFAAGMSGG+AY+YDPE     N NLE VELERLED+ DI ELKELI+NH K+TGSTVA+ IL+NW+  +  F KVMP D
Sbjct: 1404 ERFCVRNSGAKAVVEGVGDHGCEYMTGGRAVILGPTGRNFAAGMSGGIAYIYDPEDQFRINCNLEMVELERLEDS-DIVELKELIENHEKYTGSTVAQKILENWQTSVSQFVKVMPTD 1520
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BLAST of mRNA_M-pyrifera_M_contig89473.20741.1 vs. uniprot
Match: A0A3C0YHP0_9GAMM (Glutamate synthase subunit alpha (Fragment) n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3C0YHP0_9GAMM)

HSP 1 Score: 171 bits (433), Expect = 8.890e-50
Identity = 82/118 (69.49%), Postives = 99/118 (83.90%), Query Frame = 1 
Query:    1 ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLEDAEDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD 354
            ERFCVRNSGA+AVVEG+GDHGCEYMTGGR VVLGETGRNFAAGMSGG+AY++DP G    N N E V+L+ +++A DIAELK+LI NH+++TGSTVA+A+L NWE  +  F KVMP D
Sbjct:  251 ERFCVRNSGARAVVEGVGDHGCEYMTGGRVVVLGETGRNFAAGMSGGIAYIWDPRGQFAANCNQEMVDLDPMDEAVDIAELKQLISNHQQYTGSTVAEALLANWEQSVAQFIKVMPQD 368
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BLAST of mRNA_M-pyrifera_M_contig89473.20741.1 vs. uniprot
Match: A0A357TFT4_9BACT (GXGXG domain-containing protein (Fragment) n=1 Tax=Verrucomicrobiales bacterium TaxID=2026801 RepID=A0A357TFT4_9BACT)

HSP 1 Score: 163 bits (412), Expect = 1.510e-49
Identity = 80/118 (67.80%), Postives = 95/118 (80.51%), Query Frame = 1 
Query:    1 ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLEDAEDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD 354
            ERFCVRNSGA AVVEG+GDHGCEYMTGGRAV+LG  GRNFAAGMSGGVAY++DPE +   N N+E VELE++ED +DIAEL+ LI+ H + TGS+VA  +L+NW   L  F KVMP D
Sbjct:    6 ERFCVRNSGASAVVEGVGDHGCEYMTGGRAVILGPAGRNFAAGMSGGVAYIWDPENNFPPNCNMEMVELEKVEDFDDIAELQGLIEEHAERTGSSVATEVLNNWSTALGQFVKVMPTD 123
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BLAST of mRNA_M-pyrifera_M_contig89473.20741.1 vs. uniprot
Match: A0A3B9D0K2_9PLAN (Glutamate synthase large subunit (Fragment) n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A3B9D0K2_9PLAN)

HSP 1 Score: 177 bits (449), Expect = 3.210e-49
Identity = 86/118 (72.88%), Postives = 98/118 (83.05%), Query Frame = 1 
Query:    1 ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLEDAEDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD 354
            ERFCVRNSGA AV+EG+GDHGCEYMTGGRAV+LG TGRNFAAGMSGGVAYV+DP+G  L N NL+ VELE +E  ED++ELK LI+NHR +TGS VA  ILDNW+N L  F KVMP D
Sbjct: 1057 ERFCVRNSGASAVIEGVGDHGCEYMTGGRAVILGPTGRNFAAGMSGGVAYVHDPDGKFLVNCNLDLVELEEVEAEEDVSELKSLIENHRTYTGSDVAAGILDNWDNALPQFVKVMPTD 1174
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BLAST of mRNA_M-pyrifera_M_contig89473.20741.1 vs. uniprot
Match: A0A517ZHE2_9PLAN (Ferredoxin-dependent glutamate synthase 1 n=3 Tax=Symmachiella TaxID=2795780 RepID=A0A517ZHE2_9PLAN)

HSP 1 Score: 176 bits (446), Expect = 8.230e-49
Identity = 85/118 (72.03%), Postives = 98/118 (83.05%), Query Frame = 1 
Query:    1 ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLEDAEDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD 354
            ERFC+RNSGA+AVVEG+GDHGCEYMTGGR VVLG TGRNFAAGMSGGVAY+YDP    LQN NLE VELERLE+  DI+EL+ELI  H+ +TGS VA  IL++WE  +  F+KVMPVD
Sbjct: 1391 ERFCIRNSGARAVVEGVGDHGCEYMTGGRVVVLGPTGRNFAAGMSGGVAYIYDPRDEFLQNCNLEMVELERLEEEADISELRELIQKHQNYTGSPVAARILEDWETSVSQFRKVMPVD 1508
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BLAST of mRNA_M-pyrifera_M_contig89473.20741.1 vs. uniprot
Match: A0A382BB70_9ZZZZ (GXGXG domain-containing protein n=1 Tax=marine metagenome TaxID=408172 RepID=A0A382BB70_9ZZZZ)

HSP 1 Score: 166 bits (421), Expect = 9.810e-49
Identity = 82/118 (69.49%), Postives = 94/118 (79.66%), Query Frame = 1 
Query:    1 ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAYVYDPEGHLLQNSNLETVELERLEDAEDIAELKELIDNHRKFTGSTVAKAILDNWENELELFKKVMPVD 354
            ERFCVRNSGA+AV+EG+GDHGCEYMTGGRAV+LG TGRNFAAGMSGGVAY++DP+     N N+E VELE +EDAEDI EL+ LI  H + TGSTVA  +LDNW   L  F KVMP D
Sbjct:  178 ERFCVRNSGAQAVIEGVGDHGCEYMTGGRAVILGPTGRNFAAGMSGGVAYIWDPDNAFAPNCNMEMVELESVEDAEDITELQSLIGEHAERTGSTVAAELLDNWSTVLGQFVKVMPTD 295
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig89473.20741.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8BAF9_9PLAN1.300e-6899.15Glutamate synthase large subunit n=26 Tax=Planctom... [more]
A0A3D3RG18_9PLAN1.990e-6287.29Glutamate synthase subunit alpha (Fragment) n=1 Ta... [more]
A0A517SMN7_9PLAN6.560e-5276.27Ferredoxin-dependent glutamate synthase 1 n=1 Tax=... [more]
A0A7C2PGV5_9PLAN2.890e-5072.03Glutamate synthase subunit alpha n=1 Tax=Schlesner... [more]
UPI001420E1E35.050e-5075.42glutamate synthase large subunit n=1 Tax=Rubinisph... [more]
A0A3C0YHP0_9GAMM8.890e-5069.49Glutamate synthase subunit alpha (Fragment) n=1 Ta... [more]
A0A357TFT4_9BACT1.510e-4967.80GXGXG domain-containing protein (Fragment) n=1 Tax... [more]
A0A3B9D0K2_9PLAN3.210e-4972.88Glutamate synthase large subunit (Fragment) n=1 Ta... [more]
A0A517ZHE2_9PLAN8.230e-4972.03Ferredoxin-dependent glutamate synthase 1 n=3 Tax=... [more]
A0A382BB70_9ZZZZ9.810e-4969.49GXGXG domain-containing protein n=1 Tax=marine met... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig89473contigM-pyrifera_M_contig89473:1..354 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score210.3
Seed ortholog evalue4.9e-52
Seed eggNOG ortholog344747.PM8797T_04710
Preferred namegltB
KEGG rclassRC00006,RC00010,RC02799
KEGG koko:K00265,ko:K00284
KEGG ReactionR00021,R00093,R00114,R00248,R10086
KEGG Pathwayko00250,ko00630,ko00910,ko01100,ko01110,ko01120,ko01130,ko01230,map00250,map00630,map00910,map01100,map01110,map01120,map01130,map01230
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0005886,GO:0006082,GO:0006520,GO:0006536,GO:0006537,GO:0006541,GO:0006807,GO:0008150,GO:0008152,GO:0008652,GO:0009058,GO:0009064,GO:0009084,GO:0009987,GO:0015930,GO:0016020,GO:0016053,GO:0016491,GO:0016638,GO:0019676,GO:0019740,GO:0019752,GO:0040007,GO:0043436,GO:0043648,GO:0043650,GO:0044237,GO:0044238,GO:0044249,GO:0044281,GO:0044283,GO:0044424,GO:0044444,GO:0044464,GO:0046394,GO:0055114,GO:0071704,GO:0071944,GO:1901564,GO:1901566,GO:1901576,GO:1901605,GO:1901607
EggNOG free text desc.glutamate synthase
EggNOG OGs2IXKD@203682,COG0067@1,COG0067@2,COG0069@1,COG0069@2,COG0070@1,COG0070@2
EC1.4.1.13,1.4.1.14,1.4.7.1
COG Functional cat.E
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size354
Cds size354
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816805.0523534-CDS-M-pyrifera_M_contig89473:0..3541622816805.0523534-CDS-M-pyrifera_M_contig89473:0..354Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89473 1..354 -
1692278188.8245754-CDS-M-pyrifera_M_contig89473:0..3541692278188.8245754-CDS-M-pyrifera_M_contig89473:0..354Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89473 1..354 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig89473.20741.1prot_M-pyrifera_M_contig89473.20741.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig89473 1..354 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig89473.20741.1

>prot_M-pyrifera_M_contig89473.20741.1 ID=prot_M-pyrifera_M_contig89473.20741.1|Name=mRNA_M-pyrifera_M_contig89473.20741.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=118bp
ERFCVRNSGAKAVVEGIGDHGCEYMTGGRAVVLGETGRNFAAGMSGGVAY
VYDPEGHLLQNSNLETVELERLEDAEDIAELKELIDNHRKFTGSTVAKAI
LDNWENELELFKKVMPVD
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mRNA from alignment at M-pyrifera_M_contig89473:1..354-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig89473.20741.1 ID=mRNA_M-pyrifera_M_contig89473.20741.1|Name=mRNA_M-pyrifera_M_contig89473.20741.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=354bp|location=Sequence derived from alignment at M-pyrifera_M_contig89473:1..354- (Macrocystis pyrifera P11B4 male)
GAACGTTTCTGTGTGCGTAACTCCGGAGCGAAAGCGGTTGTGGAAGGAAT
CGGCGATCACGGTTGTGAATACATGACCGGCGGTCGGGCCGTTGTTCTCG
GTGAGACCGGACGCAACTTTGCAGCCGGGATGTCGGGTGGTGTGGCTTAT
GTCTACGACCCCGAAGGTCATCTGCTGCAGAACAGCAATCTGGAGACCGT
GGAACTGGAAAGGCTGGAAGATGCCGAGGATATCGCCGAACTGAAAGAGC
TGATTGACAATCATCGCAAGTTCACCGGTTCGACCGTGGCCAAAGCGATT
CTGGATAACTGGGAAAACGAACTGGAACTGTTCAAGAAAGTCATGCCTGT
GGAT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig89473:1..354-

>mRNA_M-pyrifera_M_contig89473.20741.1 ID=mRNA_M-pyrifera_M_contig89473.20741.1|Name=mRNA_M-pyrifera_M_contig89473.20741.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=708bp|location=Sequence derived from alignment at M-pyrifera_M_contig89473:1..354- (Macrocystis pyrifera P11B4 male)
GAACGTTTCTGTGTGCGTAACTCCGGAGCGAAAGCGGTTGTGGAAGGAAT
CGGCGATCACGGTTGTGAATACATGACCGGCGGTCGGGCCGTTGTTCTCG
GTGAGACCGGACGCAACTTTGCAGCCGGGATGTCGGGTGGTGTGGCTTAT
GTCTACGACCCCGAAGGTCATCTGCTGCAGAACAGCAATCTGGAGACCGT
GGAACTGGAAAGGCTGGAAGATGCCGAGGATATCGCCGAACTGAAAGAGC
TGATTGACAATCATCGCAAGTTCACCGGTTCGACCGTGGCCAAAGCGATT
CTGGATAACTGGGAAAACGAACTGGAACTGTTCAAGAAAGTCATGCCTGT
GGATGAACGTTTCTGTGTGCGTAACTCCGGAGCGAAAGCGGTTGTGGAAG
GAATCGGCGATCACGGTTGTGAATACATGACCGGCGGTCGGGCCGTTGTT
CTCGGTGAGACCGGACGCAACTTTGCAGCCGGGATGTCGGGTGGTGTGGC
TTATGTCTACGACCCCGAAGGTCATCTGCTGCAGAACAGCAATCTGGAGA
CCGTGGAACTGGAAAGGCTGGAAGATGCCGAGGATATCGCCGAACTGAAA
GAGCTGATTGACAATCATCGCAAGTTCACCGGTTCGACCGTGGCCAAAGC
GATTCTGGATAACTGGGAAAACGAACTGGAACTGTTCAAGAAAGTCATGC
CTGTGGAT
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