mRNA_M-pyrifera_M_contig89398.20717.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig89398.20717.1
Unique NamemRNA_M-pyrifera_M_contig89398.20717.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig89398.20717.1 vs. uniprot
Match: A0A8J4TCB8_CLAMG (2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 n=1 Tax=Clarias magur TaxID=1594786 RepID=A0A8J4TCB8_CLAMG)

HSP 1 Score: 108 bits (269), Expect = 5.170e-26
Identity = 63/141 (44.68%), Postives = 85/141 (60.28%), Query Frame = 1
Query:   43 REKLVRMEAARRSAIERLHTPLHRELYRFSPSFLSPEFRAFVEESEKGEATREDVRELVTRLAVEEESNVGVYSIPLLTEEFCKRLCEELEHLEASGLPLVRPNSMNSYGIVLTEVGFDH-LLTGLRERVVEPLASALFTD 462
            R  L    A R+S I   + PLH+ +Y    S+L+PEF   VE S    A+++ V EL+T      E+   VY  P+ T+EFCK L EELEH E S  P  RPN+MN+YGI+L E+GFD  L+T LRE  ++PL + L+ D
Sbjct:   65 RRDLSTQSAKRKSIIAETYKPLHQHVYSLLESYLAPEFVEIVEYSRGDSASKDGVLELITT-----EAAPRVYRFPVFTQEFCKDLLEELEHFERSEAPKGRPNTMNNYGILLNELGFDEGLITPLRELYLKPLCALLYPD 200          
BLAST of mRNA_M-pyrifera_M_contig89398.20717.1 vs. uniprot
Match: A0A5N5KDS2_PANHP (Fe2OG dioxygenase domain-containing protein n=2 Tax=Siluroidei TaxID=1489793 RepID=A0A5N5KDS2_PANHP)

HSP 1 Score: 109 bits (272), Expect = 8.960e-26
Identity = 62/141 (43.97%), Postives = 84/141 (59.57%), Query Frame = 1
Query:   43 REKLVRMEAARRSAIERLHTPLHRELYRFSPSFLSPEFRAFVEESEKGEATREDVRELVTRLAVEEESNVGVYSIPLLTEEFCKRLCEELEHLEASGLPLVRPNSMNSYGIVLTEVGFDH-LLTGLRERVVEPLASALFTD 462
            R  L    A R+S I   + PLH+ +YR   SF +PEF   V+   +  A+ E V EL+T      E+   VY  P+ T++FCK L EELEH E S  P  RPN+MN+YGI+L E+GFD  L+  LRE  ++PL+S L+ D
Sbjct:   65 RRNLAAQSAERKSIIRETYKPLHQHVYRLQESFFAPEFLEIVKYCREDGASEEGVLELITA-----EAAPRVYRFPVFTQDFCKELVEELEHFERSEAPKGRPNTMNNYGILLNELGFDEGLMAPLRELYLKPLSSLLYPD 200          
BLAST of mRNA_M-pyrifera_M_contig89398.20717.1 vs. uniprot
Match: A0A3Q3WVX4_MOLML (2-oxoglutarate and iron-dependent oxygenase domain containing 2 n=1 Tax=Mola mola TaxID=94237 RepID=A0A3Q3WVX4_MOLML)

HSP 1 Score: 107 bits (266), Expect = 7.000e-25
Identity = 61/141 (43.26%), Postives = 84/141 (59.57%), Query Frame = 1
Query:   43 REKLVRMEAARRSAIERLHTPLHRELYRFSPSFLSPEFRAFVEESEKGEATREDVRELVTRLAVEEESNVGVYSIPLLTEEFCKRLCEELEHLEASGLPLVRPNSMNSYGIVLTEVGFDH-LLTGLRERVVEPLASALFTD 462
            R +L  M A R +AI+  + PLH  +Y    SFL+P+F+  VE     +A ++ + +L+     EEE+ V VY  P+    FC  L EELEH E S  P  RPN+MN YGI+L E+GFD   +T LRER ++PL S L+ D
Sbjct:   63 RRRLGAMSAERAAAIKDAYRPLHPHVYHLKESFLAPKFKHIVEHCRGTDAGKDGLLDLL-----EEEAAVQVYRFPVFERSFCDELLEELEHFERSSAPKGRPNTMNHYGILLNELGFDEGFITPLRERYLQPLTSVLYPD 198          
BLAST of mRNA_M-pyrifera_M_contig89398.20717.1 vs. uniprot
Match: I3KGF9_ORENI (Fe2OG dioxygenase domain-containing protein n=15 Tax=Pseudocrenilabrinae TaxID=318546 RepID=I3KGF9_ORENI)

HSP 1 Score: 107 bits (266), Expect = 7.880e-25
Identity = 62/145 (42.76%), Postives = 90/145 (62.07%), Query Frame = 1
Query:   34 QVVREKLVRMEAARRSA-IERLHTPLHRELYRFSPSFLSPEFRAFVEESEKGEATREDVRELVTRLAVEEESNVGVYSIPLLTEEFCKRLCEELEHLEASGLPLVRPNSMNSYGIVLTEVGFD-HLLTGLRERVVEPLASALFTD 462
            +V R K + + +A+R+A I+ ++ PLH  +Y    S+L+ EF+  VE  +   AT E + +L+     EE++   VY  PL  + FC+ L EELEH E SG P  RPN+MN YGI+L E+GFD H +T LRE+ + PL S L+ D
Sbjct:   68 EVARRKYLGVTSAQRAAAIKDMYQPLHPHVYHLQESYLASEFKQIVEYCQSSNATEEGLLDLL-----EEQAAPRVYRFPLFDKSFCEDLMEELEHFEQSGAPKGRPNTMNQYGILLNELGFDEHFITPLREQYLLPLTSLLYPD 207          
BLAST of mRNA_M-pyrifera_M_contig89398.20717.1 vs. uniprot
Match: UPI000F4D8D69 (2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 n=1 Tax=Tachysurus fulvidraco TaxID=1234273 RepID=UPI000F4D8D69)

HSP 1 Score: 106 bits (265), Expect = 9.630e-25
Identity = 61/141 (43.26%), Postives = 87/141 (61.70%), Query Frame = 1
Query:   43 REKLVRMEAARRSAIERLHTPLHRELYRFSPSFLSPEFRAFVEESEKGEATREDVRELVTRLAVEEESNVGVYSIPLLTEEFCKRLCEELEHLEASGLPLVRPNSMNSYGIVLTEVGFDH-LLTGLRERVVEPLASALFTD 462
            R  L    A R+S I+  + PLH+ +Y    SFL+PEF   V+ S +  A+ E + +L+T      E+   VY  P+ T++FCK L EELEH E S  P  RPN+MN+YGI+L E+GFD  L+T LRE  ++P++S L+ D
Sbjct:   67 RRNLETQSAERKSIIKETYKPLHQHVYSLQESFLAPEFVEIVKYSLEDGASEEGLLKLITS-----EAAPRVYRFPVFTQDFCKDLVEELEHFECSEAPKGRPNTMNNYGILLNELGFDEGLMTPLRELYLKPISSLLYPD 202          
BLAST of mRNA_M-pyrifera_M_contig89398.20717.1 vs. uniprot
Match: A0A2G8KCE5_STIJA (Putative 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 isoform X1 n=2 Tax=Stichopus japonicus TaxID=307972 RepID=A0A2G8KCE5_STIJA)

HSP 1 Score: 105 bits (262), Expect = 2.360e-24
Identity = 59/129 (45.74%), Postives = 80/129 (62.02%), Query Frame = 1
Query:   73 RRSAIERLHTPLHRELYRFSPSFLSPEFRAFVEESEKGEATREDVRELVTRLAVEEESNVGVYSIPLLTEEFCKRLCEELEHLEASGLPLVRPNSMNSYGIVLTEVGFDH-LLTGLRERVVEPLASALF 456
            RR  +   + PLH+ LY+    FLSP+F+  VE +    AT+E + + ++  A E      VYS P+ TE+FC+ L EEL H E S LP  RPN+MN+YGI+L E+GFD   LT LRER + PL+  LF
Sbjct:   69 RRREVACRYKPLHQHLYKLEDKFLSPKFKELVEYASSHHATKEGLVKAISSEAAER-----VYSFPVFTEDFCRELIEELIHFEQSDLPKGRPNTMNNYGILLNELGFDEEFLTPLRERYLTPLSRLLF 192          
BLAST of mRNA_M-pyrifera_M_contig89398.20717.1 vs. uniprot
Match: A0A671W6X6_SPAAU (2-oxoglutarate and iron dependent oxygenase domain containing 2 n=5 Tax=Sparidae TaxID=8169 RepID=A0A671W6X6_SPAAU)

HSP 1 Score: 105 bits (261), Expect = 4.780e-24
Identity = 60/141 (42.55%), Postives = 85/141 (60.28%), Query Frame = 1
Query:   43 REKLVRMEAARRSAIERLHTPLHRELYRFSPSFLSPEFRAFVEESEKGEATREDVRELVTRLAVEEESNVGVYSIPLLTEEFCKRLCEELEHLEASGLPLVRPNSMNSYGIVLTEVGFDH-LLTGLRERVVEPLASALFTD 462
            R +L    A R +AI+ ++ PLH  +Y    S+L+P+F+  VE    G+++ E + EL+     EEE+ V VY  P+  + FC  L EELEH E S  P  RPN+MN YGI+L E+GFD   +T LRE+ + PL S L+ D
Sbjct:   83 RRRLSATSAERAAAIKDVYRPLHPHVYHLQESYLAPKFKQIVEYCRGGDSSVEGLLELL-----EEEAAVRVYRFPVFEKSFCDELVEELEHFEKSSAPKGRPNTMNHYGILLNELGFDEGFVTPLREQYLRPLTSLLYPD 218          
BLAST of mRNA_M-pyrifera_M_contig89398.20717.1 vs. uniprot
Match: A0A3B1JWZ6_ASTMX (2-oxoglutarate and iron dependent oxygenase domain containing 2 n=2 Tax=Astyanax mexicanus TaxID=7994 RepID=A0A3B1JWZ6_ASTMX)

HSP 1 Score: 104 bits (259), Expect = 7.560e-24
Identity = 57/133 (42.86%), Postives = 84/133 (63.16%), Query Frame = 1
Query:   67 AARRSAIERLHTPLHRELYRFSPSFLSPEFRAFVEESEKGEATREDVRELVTRLAVEEESNVGVYSIPLLTEEFCKRLCEELEHLEASGLPLVRPNSMNSYGIVLTEVGFDH-LLTGLRERVVEPLASALFTD 462
            A R++AI+  + PLH+ +Y    S+L+PEF   V+  +   AT+E + +L+       E+   VY  P+ T+ FC+ L EELEH E S  P  RPN+MN+YGI+L E+GFD  L+T LRER + PL++ L+ D
Sbjct:   75 AERKAAIKEKYRPLHQHVYCLQESYLTPEFLQIVKYCQSDGATQEGLMKLI-----HSEAAPRVYRFPIFTKNFCRDLIEELEHFEESDAPKGRPNTMNNYGILLNELGFDEGLITPLRERYLRPLSALLYPD 202          
BLAST of mRNA_M-pyrifera_M_contig89398.20717.1 vs. uniprot
Match: UPI001ED8277B (2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 isoform X1 n=5 Tax=Scatophagus argus TaxID=75038 RepID=UPI001ED8277B)

HSP 1 Score: 103 bits (258), Expect = 1.130e-23
Identity = 56/135 (41.48%), Postives = 82/135 (60.74%), Query Frame = 1
Query:   61 MEAARRSAIERLHTPLHRELYRFSPSFLSPEFRAFVEESEKGEATREDVRELVTRLAVEEESNVGVYSIPLLTEEFCKRLCEELEHLEASGLPLVRPNSMNSYGIVLTEVGFDHL-LTGLRERVVEPLASALFTD 462
            M A R +AI+ ++ PLH  +Y    S  +P+F+  VE   +G+ + E + +L+     E+E+ + VY  P+  + FC+ L EELEH E S  P  RPN+MN YGI+L E+GFD   +T LRER + PL S L+ D
Sbjct:   78 MSAERAAAIKEVYRPLHPHVYHLQESCFAPKFKQIVEYCRRGDTSDEGLLDLL-----EKEAALRVYRFPMFEKSFCEELLEELEHFEQSSAPKGRPNTMNHYGILLNELGFDEFFITPLRERYLHPLTSVLYPD 207          
BLAST of mRNA_M-pyrifera_M_contig89398.20717.1 vs. uniprot
Match: A0A8C9V7B5_SCLFO (2-oxoglutarate and iron dependent oxygenase domain containing 2 n=2 Tax=Scleropages formosus TaxID=113540 RepID=A0A8C9V7B5_SCLFO)

HSP 1 Score: 103 bits (258), Expect = 1.180e-23
Identity = 62/156 (39.74%), Postives = 95/156 (60.90%), Query Frame = 1
Query:    1 ELGHEERAAQMQVVREKLVRMEAARRSA-IERLHTPLHRELYRFSPSFLSPEFRAFVEESEKGEATREDVRELVTRLAVEEESNVGVYSIPLLTEEFCKRLCEELEHLEASGLPLVRPNSMNSYGIVLTEVGFDH-LLTGLRERVVEPLASALFTD 462
            +LG+     + +V R + + +++A R+A I+ L+ PLH  +Y    SFL+PEF   V+ S    AT+E + + +  LA +      VY  P+  +EFC+ L EELEH E S  P  RPN+MN+YG++L E+GFD   LT LRE+ ++P++  LF D
Sbjct:   58 QLGNVLNKIEAEVGRRRQLCVKSAERAAWIKELYKPLHSHVYSLQESFLAPEFLEIVKYSCSDGATKEGLFDYIEPLAAQR-----VYRFPVFRKEFCRELIEELEHFEQSEAPKGRPNTMNNYGVLLNELGFDEQFLTPLREKYLQPISQLLFPD 208          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig89398.20717.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A8J4TCB8_CLAMG5.170e-2644.682-oxoglutarate and iron-dependent oxygenase domain... [more]
A0A5N5KDS2_PANHP8.960e-2643.97Fe2OG dioxygenase domain-containing protein n=2 Ta... [more]
A0A3Q3WVX4_MOLML7.000e-2543.262-oxoglutarate and iron-dependent oxygenase domain... [more]
I3KGF9_ORENI7.880e-2542.76Fe2OG dioxygenase domain-containing protein n=15 T... [more]
UPI000F4D8D699.630e-2543.262-oxoglutarate and iron-dependent oxygenase domain... [more]
A0A2G8KCE5_STIJA2.360e-2445.74Putative 2-oxoglutarate and iron-dependent oxygena... [more]
A0A671W6X6_SPAAU4.780e-2442.552-oxoglutarate and iron dependent oxygenase domain... [more]
A0A3B1JWZ6_ASTMX7.560e-2442.862-oxoglutarate and iron dependent oxygenase domain... [more]
UPI001ED8277B1.130e-2341.482-oxoglutarate and iron-dependent oxygenase domain... [more]
A0A8C9V7B5_SCLFO1.180e-2339.742-oxoglutarate and iron dependent oxygenase domain... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig89398contigM-pyrifera_M_contig89398:280..741 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score111.3
Seed ortholog evalue3.8e-22
Seed eggNOG ortholog8128.ENSONIP00000020204
Preferred nameOGFOD2
Hectar predicted targeting categoryother localisation
EggNOG free text desc.2-oxoglutarate and iron-dependent oxygenase
EggNOG OGs38HYK@33154,3BEZF@33208,3CZ82@33213,484CA@7711,48XD8@7742,49Z07@7898,KOG1971@1,KOG1971@2759
COG Functional cat.O
Best tax levelActinopterygii
Best eggNOG OGNA|NA|NA
Exons1
Model size462
Cds size432
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816803.9431899-UTR-M-pyrifera_M_contig89398:279..3091622816803.9431899-UTR-M-pyrifera_M_contig89398:279..309Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig89398 280..309 +
1692278188.2315767-UTR-M-pyrifera_M_contig89398:279..3091692278188.2315767-UTR-M-pyrifera_M_contig89398:279..309Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig89398 280..309 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816803.954284-CDS-M-pyrifera_M_contig89398:309..7411622816803.954284-CDS-M-pyrifera_M_contig89398:309..741Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89398 310..741 +
1692278188.2419033-CDS-M-pyrifera_M_contig89398:309..7411692278188.2419033-CDS-M-pyrifera_M_contig89398:309..741Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89398 310..741 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig89398.20717.1prot_M-pyrifera_M_contig89398.20717.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig89398 310..741 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig89398.20717.1

>prot_M-pyrifera_M_contig89398.20717.1 ID=prot_M-pyrifera_M_contig89398.20717.1|Name=mRNA_M-pyrifera_M_contig89398.20717.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=144bp
MQVVREKLVRMEAARRSAIERLHTPLHRELYRFSPSFLSPEFRAFVEESE
KGEATREDVRELVTRLAVEEESNVGVYSIPLLTEEFCKRLCEELEHLEAS
GLPLVRPNSMNSYGIVLTEVGFDHLLTGLRERVVEPLASALFTD
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mRNA from alignment at M-pyrifera_M_contig89398:280..741+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig89398.20717.1 ID=mRNA_M-pyrifera_M_contig89398.20717.1|Name=mRNA_M-pyrifera_M_contig89398.20717.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=462bp|location=Sequence derived from alignment at M-pyrifera_M_contig89398:280..741+ (Macrocystis pyrifera P11B4 male)
GAGCTGGGCCACGAAGAGAGGGCCGCACAGATGCAGGTGGTCAGGGAGAA GCTCGTGCGCATGGAGGCGGCACGGCGCTCAGCCATCGAGAGGCTCCACA CTCCTCTCCACCGTGAACTCTACAGATTCTCGCCTTCTTTTCTGTCGCCA GAGTTCCGGGCGTTTGTGGAGGAGAGTGAGAAGGGAGAGGCGACCCGTGA GGACGTGCGAGAGTTGGTCACACGTTTGGCAGTGGAGGAGGAGAGCAACG TGGGAGTCTACAGTATACCATTGCTCACTGAGGAGTTCTGCAAGAGGCTG TGCGAGGAGCTGGAGCACTTGGAGGCCTCAGGCCTGCCTTTGGTGCGGCC AAACTCCATGAACAGCTATGGCATAGTCCTAACGGAGGTTGGCTTCGACC ACCTCTTGACAGGACTGCGAGAGAGGGTTGTAGAACCGCTGGCAAGCGCT CTCTTCACTGAC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig89398:280..741+

>mRNA_M-pyrifera_M_contig89398.20717.1 ID=mRNA_M-pyrifera_M_contig89398.20717.1|Name=mRNA_M-pyrifera_M_contig89398.20717.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=864bp|location=Sequence derived from alignment at M-pyrifera_M_contig89398:280..741+ (Macrocystis pyrifera P11B4 male)
ATGCAGGTGGTCAGGGAGAAGCTCGTGCGCATGGAGGCGGCACGGCGCTC
AGCCATCGAGAGGCTCCACACTCCTCTCCACCGTGAACTCTACAGATTCT
CGCCTTCTTTTCTGTCGCCAGAGTTCCGGGCGTTTGTGGAGGAGAGTGAG
AAGGGAGAGGCGACCCGTGAGGACGTGCGAGAGTTGGTCACACGTTTGGC
AGTGGAGGAGGAGAGCAACGTGGGAGTCTACAGTATACCATTGCTCACTG
AGGAGTTCTGCAAGAGGCTGTGCGAGGAGCTGGAGCACTTGGAGGCCTCA
GGCCTGCCTTTGGTGCGGCCAAACTCCATGAACAGCTATGGCATAGTCCT
AACGGAGGTTGGCTTCGACCACCTCTTGACAGGACTGCGAGAGAGGGTTG
TAGAACCGCTGGCAAGCGCTCTCTTCACTGACATGCAGGTGGTCAGGGAG
AAGCTCGTGCGCATGGAGGCGGCACGGCGCTCAGCCATCGAGAGGCTCCA
CACTCCTCTCCACCGTGAACTCTACAGATTCTCGCCTTCTTTTCTGTCGC
CAGAGTTCCGGGCGTTTGTGGAGGAGAGTGAGAAGGGAGAGGCGACCCGT
GAGGACGTGCGAGAGTTGGTCACACGTTTGGCAGTGGAGGAGGAGAGCAA
CGTGGGAGTCTACAGTATACCATTGCTCACTGAGGAGTTCTGCAAGAGGC
TGTGCGAGGAGCTGGAGCACTTGGAGGCCTCAGGCCTGCCTTTGGTGCGG
CCAAACTCCATGAACAGCTATGGCATAGTCCTAACGGAGGTTGGCTTCGA
CCACCTCTTGACAGGACTGCGAGAGAGGGTTGTAGAACCGCTGGCAAGCG
CTCTCTTCACTGAC
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