mRNA_M-pyrifera_M_contig89121.20663.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig89121.20663.1
Unique NamemRNA_M-pyrifera_M_contig89121.20663.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig89121.20663.1 vs. uniprot
Match: A0A167TSP2_9PEZI (Ceramide very long chain fatty acid hydroxylase n=1 Tax=Sporothrix insectorum RCEF 264 TaxID=1081102 RepID=A0A167TSP2_9PEZI)

HSP 1 Score: 110 bits (275), Expect = 5.960e-26
Identity = 57/147 (38.78%), Postives = 82/147 (55.78%), Query Frame = 1
Query:    1 PSRDQPLYTVPLVWTPVLASTLCLVVVAGAQVSLLPAHFLLGMLVWTVIEYVMHRCLFHSNALVVDHPIGIGAHFLLHGAHHLLPQHTDRLLFPPLMGVFAAAVLFLPFHRLFSYAFGLGTPDFAVVYTGAVLGFIVYDVLHFCIHH 441
            P    P + VPL+W P +A  + L     +      A F  G  +WT++EYV+HRCLFH +  + D+ +GI  HFLLHG HH LP    RL+ PP + V  A   +   H LF+Y + + T     V+ G +LG+I YD+ H+ +HH
Sbjct:  201 PLSMTPWWMVPLLWLPCVAYGVYLASPGFSSPLYQAAFFAFGFFIWTLVEYVLHRCLFHLDYYLPDNRVGITLHFLLHGIHHYLPMDKYRLVMPPTLFVVLATPFWKLAHTLFAYDWNVAT----TVFCGGILGYICYDLTHYFLHH 343          
BLAST of mRNA_M-pyrifera_M_contig89121.20663.1 vs. uniprot
Match: A0A1V1T270_9FUNG (Ceramide very long chain fatty acid hydroxylase n=3 Tax=Fungi TaxID=4751 RepID=A0A1V1T270_9FUNG)

HSP 1 Score: 110 bits (274), Expect = 6.570e-26
Identity = 58/147 (39.46%), Postives = 81/147 (55.10%), Query Frame = 1
Query:    1 PSRDQPLYTVPLVWTPVLASTLCLVVVAGAQVSLLPAHFLLGMLVWTVIEYVMHRCLFHSNALVVDHPIGIGAHFLLHGAHHLLPQHTDRLLFPPLMGVFAAAVLFLPFHRLFSYAFGLGTPDFAVVYTGAVLGFIVYDVLHFCIHH 441
            P    P + VPL+W P  A    L          L A +LLG+ +WT++EY++HRCLFH +  + D+ +GI  HFLLHG HH LP    RL+ PP + V    VL +PF +L    F         VY G + G+I+YD+ H+ +HH
Sbjct:  185 PLSLTPWWVVPLLWLPADAYGTYLAAQGFQDYGTLVASWLLGLFLWTLVEYILHRCLFHLDYYLPDNRVGITLHFLLHGVHHYLPMDKYRLVMPPTLFV----VLAMPFWKLAHSVFYFNWHMATAVYCGGIFGYIMYDMTHYFLHH 327          
BLAST of mRNA_M-pyrifera_M_contig89121.20663.1 vs. uniprot
Match: UPI0020077FDF (4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase n=1 Tax=Purpureocillium takamizusanense TaxID=2060973 RepID=UPI0020077FDF)

HSP 1 Score: 108 bits (271), Expect = 1.950e-25
Identity = 57/147 (38.78%), Postives = 83/147 (56.46%), Query Frame = 1
Query:    1 PSRDQPLYTVPLVWTPVLASTLCLVVVAGAQVSLLPAHFLLGMLVWTVIEYVMHRCLFHSNALVVDHPIGIGAHFLLHGAHHLLPQHTDRLLFPPLMGVFAAAVLFLPFHRLFSYAFGLGTPDFAVVYTGAVLGFIVYDVLHFCIHH 441
            P    P + VP +W P +   + L         +  A+++ G+L+WT+IEY MHR LFH +  + D+ +GI AHFLLHG HH LP    RL+ PP++ V    VL  PF +L+ + F         VY G+V G+I YD+ H+ +HH
Sbjct:  192 PLTKTPWWMVPTIWLPCVCYGVYLANQGHESPLITAAYWVFGVLLWTLIEYCMHRFLFHIDDYLPDNRVGITAHFLLHGIHHYLPMDKYRLVMPPVLFV----VLATPFWKLYQFVFAYNWYVGTAVYCGSVFGYICYDLTHYFLHH 334          
BLAST of mRNA_M-pyrifera_M_contig89121.20663.1 vs. uniprot
Match: A0A0F4ZD73_9PEZI (Ceramide very long chain fatty acid hydroxylase n=1 Tax=Thielaviopsis punctulata TaxID=72032 RepID=A0A0F4ZD73_9PEZI)

HSP 1 Score: 108 bits (269), Expect = 3.500e-25
Identity = 57/147 (38.78%), Postives = 81/147 (55.10%), Query Frame = 1
Query:    1 PSRDQPLYTVPLVWTPVLASTLCLVVVAGAQVSLLPAHFLLGMLVWTVIEYVMHRCLFHSNALVVDHPIGIGAHFLLHGAHHLLPQHTDRLLFPPLMGVFAAAVLFLPFHRLFSYAFGLGTPDFAVVYTGAVLGFIVYDVLHFCIHH 441
            P    P + VPLVW P +   L L        SL   HF+ G  +W++IEYV+HR LFH +  + D+ + +  HFLLHG HH LP    RL+ PP + V  A   +   H LF+Y++G  T     V+ G V G++ YD+ H+ +HH
Sbjct:  187 PISLTPWWVVPLVWLPSITYALSLSAKTTTGTSL-AGHFIFGYSLWSLIEYVLHRFLFHLDEYLPDNRVALTLHFLLHGVHHYLPMDKYRLVMPPALFVVLATPFYRLAHALFTYSWGAATS----VFCGGVFGYVCYDLTHYFLHH 328          
BLAST of mRNA_M-pyrifera_M_contig89121.20663.1 vs. uniprot
Match: A0A1Y2UVI9_9PEZI (Ceramide very long chain fatty acid hydroxylase n=2 Tax=unclassified Hypoxylon TaxID=2614581 RepID=A0A1Y2UVI9_9PEZI)

HSP 1 Score: 107 bits (268), Expect = 4.820e-25
Identity = 56/147 (38.10%), Postives = 82/147 (55.78%), Query Frame = 1
Query:    1 PSRDQPLYTVPLVWTPVLASTLCLVVVAGAQVSLLPAHFLLGMLVWTVIEYVMHRCLFHSNALVVDHPIGIGAHFLLHGAHHLLPQHTDRLLFPPLMGVFAAAVLFLPFHRLFSYAFGLGTPDFAVVYTGAVLGFIVYDVLHFCIHH 441
            P    P + VPL+W P +A    L          L A ++LG+ +WT++EYV+HRCLFH +  + D+ +GI  HFLLHG HH LP    RL+ PP + +  A   +   H LF + + L T     +Y G + G+I YD+ H+ +HH
Sbjct:  185 PLSLTPWWMVPLIWLPCVAYGTYLASEGFENKLSLGASWVLGVFIWTLVEYVLHRCLFHLDDYLPDNRVGITLHFLLHGVHHYLPMDKYRLVMPPALFLVLATPFWKLAHALFYWNWHLAT----AIYCGGIFGYICYDLTHYFLHH 327          
BLAST of mRNA_M-pyrifera_M_contig89121.20663.1 vs. uniprot
Match: A0A507FDU1_9FUNG (Ceramide very long chain fatty acid hydroxylase n=1 Tax=Chytriomyces confervae TaxID=246404 RepID=A0A507FDU1_9FUNG)

HSP 1 Score: 107 bits (266), Expect = 5.550e-25
Identity = 58/143 (40.56%), Postives = 85/143 (59.44%), Query Frame = 1
Query:   16 PLYTVPLVWTPVLASTLCL-VVVAGAQVSLLPAHFLLGMLVWTVIEYVMHRCLFHSNALVVDHPIGIGAHFLLHGAHHLLPQHTDRLLFPPLMGVFAAAVLFLPFHRLFSYAFGLGTPDFAVVYTGAVLGFIVYDVLHFCIHH 441
            P + +PLV+ P+    +   V   GA ++ +PA F +G+LVWT +EY +HR LFH +  + DH I    HFLLHG HH LP    RL+ PP +G+    VL +P ++     F  GT +  VV  GA  GF++YD++H+ +HH
Sbjct:  159 PWWVIPLVYIPITLYNIYYGVTELGASLATVPAFFAVGILVWTFVEYSLHRFLFHIDEFLPDHQIAFTLHFLLHGIHHFLPMDKMRLVMPPALGL----VLSIPVYKSIIALFPTGTGN--VVAGGAYCGFMLYDLIHYHLHH 295          
BLAST of mRNA_M-pyrifera_M_contig89121.20663.1 vs. uniprot
Match: A0A4Z0YK14_9PEZI (Ceramide very long chain fatty acid hydroxylase n=1 Tax=Xylaria hypoxylon TaxID=37992 RepID=A0A4Z0YK14_9PEZI)

HSP 1 Score: 107 bits (267), Expect = 6.710e-25
Identity = 53/147 (36.05%), Postives = 83/147 (56.46%), Query Frame = 1
Query:    1 PSRDQPLYTVPLVWTPVLASTLCLVVVAGAQVSLLPAHFLLGMLVWTVIEYVMHRCLFHSNALVVDHPIGIGAHFLLHGAHHLLPQHTDRLLFPPLMGVFAAAVLFLPFHRLFSYAFGLGTPDFAVVYTGAVLGFIVYDVLHFCIHH 441
            P    P + +P++W P +A    L      +   L   +L G+L WT++EY++HR LFH +  + D+ +GI  HFLLHG HH LP    RL+ PP + +  A   +   H +FS+ + LGT     VY G + G+++YD+ H+ +HH
Sbjct:  185 PLSLTPWWMIPMLWLPAVAYGTYLAAPGFDRYGTLAVSWLSGLLTWTLLEYILHRFLFHLDHYLPDNRVGITLHFLLHGVHHYLPMDKYRLVMPPTLFLILATPFWKLAHAIFSFNWHLGT----AVYCGGIFGYVMYDMTHYFLHH 327          
BLAST of mRNA_M-pyrifera_M_contig89121.20663.1 vs. uniprot
Match: UPI0020083CA4 (inositolphosphorylceramide-B C-26 hydroxylase n=1 Tax=Xylaria bambusicola TaxID=326684 RepID=UPI0020083CA4)

HSP 1 Score: 107 bits (267), Expect = 6.710e-25
Identity = 54/147 (36.73%), Postives = 82/147 (55.78%), Query Frame = 1
Query:    1 PSRDQPLYTVPLVWTPVLASTLCLVVVAGAQVSLLPAHFLLGMLVWTVIEYVMHRCLFHSNALVVDHPIGIGAHFLLHGAHHLLPQHTDRLLFPPLMGVFAAAVLFLPFHRLFSYAFGLGTPDFAVVYTGAVLGFIVYDVLHFCIHH 441
            P    P + VPL+W P  A    L          L A +LLG+ +WT++EY++HRCLFH +  + D+ +G+  HFLLHG HH LP    RL+ PP + +  A   +   H LF + + L T     +Y G + G+++YD+ H+ +HH
Sbjct:  185 PLSMTPWWVVPLLWLPADAYGTYLASQGFQSYGNLAAAWLLGVFIWTLVEYILHRCLFHLDYYLPDNRVGLTMHFLLHGIHHYLPMDKYRLVMPPTLFLVLATPFWKLAHTLFFFNWHLAT----AIYCGGIFGYVMYDMTHYFLHH 327          
BLAST of mRNA_M-pyrifera_M_contig89121.20663.1 vs. uniprot
Match: A0A6A6W356_9PEZI (Ceramide very long chain fatty acid hydroxylase n=1 Tax=Pseudovirgaria hyperparasitica TaxID=470096 RepID=A0A6A6W356_9PEZI)

HSP 1 Score: 107 bits (267), Expect = 8.680e-25
Identity = 54/140 (38.57%), Postives = 81/140 (57.86%), Query Frame = 1
Query:   22 YTVPLVWTPVLASTLCLVVVAGAQVSLLPAHFLLGMLVWTVIEYVMHRCLFHSNALVVDHPIGIGAHFLLHGAHHLLPQHTDRLLFPPLMGVFAAAVLFLPFHRLFSYAFGLGTPDFAVVYTGAVLGFIVYDVLHFCIHH 441
            + VP VW P +A    L       +  L A++L+G+ +WT++EY +HRCLFH +  + D+ +GI AHFLLHG HH LP    RL+ PP + +  AA  +   H +F Y++         VY G + G+I YD+ H+ +HH
Sbjct:  209 WIVPTVWVPPVAYGTYLASQGMDSILQLVAYWLVGLAIWTLVEYGLHRCLFHVDHYLPDNRVGITAHFLLHGIHHYLPMDKYRLVMPPTLFIVLAAPFWKLAHAIFFYSWHAAV----AVYCGGIFGYICYDLTHYFLHH 344          
BLAST of mRNA_M-pyrifera_M_contig89121.20663.1 vs. uniprot
Match: A0A066XB20_COLSU (Ceramide very long chain fatty acid hydroxylase n=7 Tax=Colletotrichum TaxID=5455 RepID=A0A066XB20_COLSU)

HSP 1 Score: 107 bits (267), Expect = 8.780e-25
Identity = 56/147 (38.10%), Postives = 83/147 (56.46%), Query Frame = 1
Query:    1 PSRDQPLYTVPLVWTPVLASTLCLVVVAGAQVSLLPAHFLLGMLVWTVIEYVMHRCLFHSNALVVDHPIGIGAHFLLHGAHHLLPQHTDRLLFPPLMGVFAAAVLFLPFHRLFSYAFGLGTPDFAVVYTGAVLGFIVYDVLHFCIHH 441
            P    P + VPL W P +A +L L        +    +F LG+ +WT+IEY++HR LFH +  + D+ +GI AHFLLHG HH LP    RL+ PP + V  A   +   H +FSY++   T     V+ G + G++ YD+ H+ +HH
Sbjct:  207 PLSKTPWWVVPLAWLPPVAYSLYLARDGMESTTQEMLYFGLGLFLWTLIEYILHRFLFHLDQWLPDNRVGITAHFLLHGIHHYLPMDKYRLVMPPTLFVVLATPFYKLAHWVFSYSWHAAT----AVFCGGIFGYVCYDLTHYFLHH 349          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig89121.20663.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A167TSP2_9PEZI5.960e-2638.78Ceramide very long chain fatty acid hydroxylase n=... [more]
A0A1V1T270_9FUNG6.570e-2639.46Ceramide very long chain fatty acid hydroxylase n=... [more]
UPI0020077FDF1.950e-2538.784-hydroxysphinganine ceramide fatty acyl 2-hydroxy... [more]
A0A0F4ZD73_9PEZI3.500e-2538.78Ceramide very long chain fatty acid hydroxylase n=... [more]
A0A1Y2UVI9_9PEZI4.820e-2538.10Ceramide very long chain fatty acid hydroxylase n=... [more]
A0A507FDU1_9FUNG5.550e-2540.56Ceramide very long chain fatty acid hydroxylase n=... [more]
A0A4Z0YK14_9PEZI6.710e-2536.05Ceramide very long chain fatty acid hydroxylase n=... [more]
UPI0020083CA46.710e-2536.73inositolphosphorylceramide-B C-26 hydroxylase n=1 ... [more]
A0A6A6W356_9PEZI8.680e-2538.57Ceramide very long chain fatty acid hydroxylase n=... [more]
A0A066XB20_COLSU8.780e-2538.10Ceramide very long chain fatty acid hydroxylase n=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig89121contigM-pyrifera_M_contig89121:73..678 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score105.1
Seed ortholog evalue2.8e-20
Seed eggNOG ortholog1173701.A0A066XB20
Preferred nameSCS7
KEGG koko:K19703
Hectar predicted targeting categorysignal anchor
GOsGO:0000170,GO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0006082,GO:0006629,GO:0006631,GO:0006643,GO:0006644,GO:0006664,GO:0006665,GO:0006673,GO:0006675,GO:0006687,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0008610,GO:0009058,GO:0009987,GO:0012505,GO:0016020,GO:0016021,GO:0016491,GO:0019637,GO:0019752,GO:0030148,GO:0031224,GO:0031984,GO:0032787,GO:0042175,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044281,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0046467,GO:0055114,GO:0071704,GO:0080132,GO:0098827,GO:1901135,GO:1901564,GO:1901566,GO:1901576,GO:1903509
EggNOG free text desc.Belongs to the sterol desaturase family
EggNOG OGs1EYSC@1028384,218ZA@147550,38DTY@33154,3NV5W@4751,3QPMY@4890,COG3000@1,KOG0539@2759
EC1.14.18.6
COG Functional cat.I
Best tax levelGlomerellales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000
Exons3
Model size447
Cds size447
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig89121.20663.1prot_M-pyrifera_M_contig89121.20663.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig89121 73..678 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816801.567226-CDS-M-pyrifera_M_contig89121:72..1281622816801.567226-CDS-M-pyrifera_M_contig89121:72..128Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89121 73..128 -
1692278186.8936694-CDS-M-pyrifera_M_contig89121:72..1281692278186.8936694-CDS-M-pyrifera_M_contig89121:72..128Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89121 73..128 -
1622816801.5786226-CDS-M-pyrifera_M_contig89121:202..4491622816801.5786226-CDS-M-pyrifera_M_contig89121:202..449Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89121 203..449 -
1692278186.9070604-CDS-M-pyrifera_M_contig89121:202..4491692278186.9070604-CDS-M-pyrifera_M_contig89121:202..449Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89121 203..449 -
1622816801.5895858-CDS-M-pyrifera_M_contig89121:534..6781622816801.5895858-CDS-M-pyrifera_M_contig89121:534..678Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89121 535..678 -
1692278186.9198472-CDS-M-pyrifera_M_contig89121:534..6781692278186.9198472-CDS-M-pyrifera_M_contig89121:534..678Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89121 535..678 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig89121.20663.1

>prot_M-pyrifera_M_contig89121.20663.1 ID=prot_M-pyrifera_M_contig89121.20663.1|Name=mRNA_M-pyrifera_M_contig89121.20663.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=149bp
PSRDQPLYTVPLVWTPVLASTLCLVVVAGAQVSLLPAHFLLGMLVWTVIE
YVMHRCLFHSNALVVDHPIGIGAHFLLHGAHHLLPQHTDRLLFPPLMGVF
AAAVLFLPFHRLFSYAFGLGTPDFAVVYTGAVLGFIVYDVLHFCIHHCT
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mRNA from alignment at M-pyrifera_M_contig89121:73..678-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig89121.20663.1 ID=mRNA_M-pyrifera_M_contig89121.20663.1|Name=mRNA_M-pyrifera_M_contig89121.20663.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=606bp|location=Sequence derived from alignment at M-pyrifera_M_contig89121:73..678- (Macrocystis pyrifera P11B4 male)
CCATCGCGCGACCAGCCACTGTACACAGTCCCCTTGGTGTGGACACCCGT GCTGGCCTCCACCTTGTGCCTGGTCGTGGTCGCTGGCGCCCAAGTGTCGT TGCTGCCAGCACATTTCCTGCTGGGCATGCTCGTCTGGACTGTTGTACGT TGCAATACGCCCACGCAACACGCCCGCGTTGGTGGCGGCGCGTGGCCGCT CCCTGCCTTTCTTACCCCCTCCCTTGCAGATCGAGTACGTGATGCACCGG TGCCTGTTCCACTCCAACGCGCTGGTGGTCGACCATCCCATCGGGATCGG CGCCCACTTCCTCCTCCACGGAGCCCACCACCTCCTGCCCCAGCACACCG ACCGCCTCCTTTTCCCGCCCCTCATGGGTGTGTTTGCCGCGGCTGTCCTG TTCTTGCCGTTCCACCGACTCTTCTCGTACGCGTTTGGACTCGGCACGCC AGATTTCGCCGTGGTGTACACCGGAGGTGCGTGCCACGCTGCGTCGCCGT CGCTGCCGCGTCTCTGCTGGTGGCATCTGACGGGGGTTCTCGTTCCGCAG CTGTGCTTGGGTTCATCGTGTACGATGTGCTGCACTTCTGCATCCATCAC TGCACG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig89121:73..678-

>mRNA_M-pyrifera_M_contig89121.20663.1 ID=mRNA_M-pyrifera_M_contig89121.20663.1|Name=mRNA_M-pyrifera_M_contig89121.20663.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=894bp|location=Sequence derived from alignment at M-pyrifera_M_contig89121:73..678- (Macrocystis pyrifera P11B4 male)
CCATCGCGCGACCAGCCACTGTACACAGTCCCCTTGGTGTGGACACCCGT
GCTGGCCTCCACCTTGTGCCTGGTCGTGGTCGCTGGCGCCCAAGTGTCGT
TGCTGCCAGCACATTTCCTGCTGGGCATGCTCGTCTGGACTGTTCCATCG
CGCGACCAGCCACTGTACACAGTCCCCTTGGTGTGGACACCCGTGCTGGC
CTCCACCTTGTGCCTGGTCGTGGTCGCTGGCGCCCAAGTGTCGTTGCTGC
CAGCACATTTCCTGCTGGGCATGCTCGTCTGGACTGTTATCGAGTACGTG
ATGCACCGGTGCCTGTTCCACTCCAACGCGCTGGTGGTCGACCATCCCAT
CGGGATCGGCGCCCACTTCCTCCTCCACGGAGCCCACCACCTCCTGCCCC
AGCACACCGACCGCCTCCTTTTCCCGCCCCTCATGGGTGTGTTTGCCGCG
GCTGTCCTGTTCTTGCCGTTCCACCGACTCTTCTCGTACGCGTTTGGACT
CGGCACGCCAGATTTCGCCGTGGTGTACACCGGAGATCGAGTACGTGATG
CACCGGTGCCTGTTCCACTCCAACGCGCTGGTGGTCGACCATCCCATCGG
GATCGGCGCCCACTTCCTCCTCCACGGAGCCCACCACCTCCTGCCCCAGC
ACACCGACCGCCTCCTTTTCCCGCCCCTCATGGGTGTGTTTGCCGCGGCT
GTCCTGTTCTTGCCGTTCCACCGACTCTTCTCGTACGCGTTTGGACTCGG
CACGCCAGATTTCGCCGTGGTGTACACCGGAGCTGTGCTTGGGTTCATCG
TGTACGATGTGCTGCACTTCTGCATCCATCACTGCACGCTGTGCTTGGGT
TCATCGTGTACGATGTGCTGCACTTCTGCATCCATCACTGCACG
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