mRNA_M-pyrifera_M_contig88675.20591.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig88675.20591.1
Unique NamemRNA_M-pyrifera_M_contig88675.20591.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig88675.20591.1 vs. uniprot
Match: D8LRX5_ECTSI (LigB domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LRX5_ECTSI)

HSP 1 Score: 175 bits (444), Expect = 1.120e-52
Identity = 77/117 (65.81%), Postives = 94/117 (80.34%), Query Frame = 1
Query:    1 QMNADSPAAEWYRTFTGQWPKPSAIVVISAHWEERGSICVTAGAKHSLFYDYYGFPAHTYELGYDCPGEPALANRIVRMLQADGIPAKGNTARGYDHGGFSPLKLMYPDADIPVVQV 351
            Q++A S AA+WYRTFT +W KPSAIVV+SAHWE RG++ VT G KH LF+DYYGFP + Y+L Y  PG P LA RI+ +L+ADG  A+G+ +RGYDHG F PLKLMYPDAD+PVVQV
Sbjct:    3 QIDATSAAAQWYRTFTKKWAKPSAIVVVSAHWEGRGAVKVTTGEKHPLFFDYYGFPDYCYDLEYAPPGHPELARRIIELLEADGFNARGDDSRGYDHGTFIPLKLMYPDADVPVVQV 119          
BLAST of mRNA_M-pyrifera_M_contig88675.20591.1 vs. uniprot
Match: H2FST0_OCESG (Extradiol ring-cleavage dioxygenase III subunit B n=1 Tax=Oceanimonas sp. (strain GK1 / IBRC-M 10197) TaxID=511062 RepID=H2FST0_OCESG)

HSP 1 Score: 132 bits (331), Expect = 1.060e-35
Identity = 63/105 (60.00%), Postives = 75/105 (71.43%), Query Frame = 1
Query:   37 RTFTGQWPKPSAIVVISAHWEERGSICVTAGAKHSLFYDYYGFPAHTYELGYDCPGEPALANRIVRMLQADGIPAKGNTARGYDHGGFSPLKLMYPDADIPVVQV 351
            +   G+  +PSAI+VISAHWE R    VT+GA   L YDYYGFP  +Y + Y CPGEPALA+RI + LQ  GIPA  +  RGYDHG F PLKLMYP+ADIP VQ+
Sbjct:   33 QALAGKLERPSAILVISAHWEARVPT-VTSGATPGLIYDYYGFPPESYSIRYPCPGEPALAHRICQALQEAGIPASEDEQRGYDHGLFVPLKLMYPEADIPCVQL 136          
BLAST of mRNA_M-pyrifera_M_contig88675.20591.1 vs. uniprot
Match: A0A2E7E826_9GAMM (Dioxygenase n=1 Tax=Oceanospirillaceae bacterium TaxID=1899355 RepID=A0A2E7E826_9GAMM)

HSP 1 Score: 130 bits (327), Expect = 2.860e-35
Identity = 62/101 (61.39%), Postives = 75/101 (74.26%), Query Frame = 1
Query:   49 GQWPKPSAIVVISAHWEERGSICVTAGAKHSLFYDYYGFPAHTYELGYDCPGEPALANRIVRMLQADGIPAKGNTARGYDHGGFSPLKLMYPDADIPVVQV 351
            G+ PKP+AI+VISAHWEER    +TAGA   L YDYYGFP  +YE+ Y  PGE  LA+RI + L+  GI A+ +  RG+DHG F PLKLMYPDADIPVVQ+
Sbjct:   21 GEIPKPTAILVISAHWEERVPT-ITAGANPDLIYDYYGFPPQSYEIRYPAPGETTLASRIHQALEQSGIHARMDNKRGFDHGVFVPLKLMYPDADIPVVQL 120          
BLAST of mRNA_M-pyrifera_M_contig88675.20591.1 vs. uniprot
Match: A0A2M7G2W7_9BACT (Dioxygenase n=1 Tax=bacterium (Candidatus Blackallbacteria) CG17_big_fil_post_rev_8_21_14_2_50_48_46 TaxID=2014261 RepID=A0A2M7G2W7_9BACT)

HSP 1 Score: 130 bits (327), Expect = 3.750e-35
Identity = 58/109 (53.21%), Postives = 71/109 (65.14%), Query Frame = 1
Query:   25 AEWYRTFTGQWPKPSAIVVISAHWEERGSICVTAGAKHSLFYDYYGFPAHTYELGYDCPGEPALANRIVRMLQADGIPAKGNTARGYDHGGFSPLKLMYPDADIPVVQV 351
            A+W +      P P AIVV+SAHWE    + +T  A+  L YDYYGFP HTY+L Y  PG P LA +I   L A  IPA+ +  RGYDHG F PLKL+YP+A IPVVQ+
Sbjct:   33 ADWLKNLRHTLPVPQAIVVVSAHWENSAEVLITGHAQPPLLYDYYGFPEHTYQLSYPAPGAPDLAGKIQTHLAAQAIPARIDAERGYDHGVFIPLKLIYPEAQIPVVQI 141          
BLAST of mRNA_M-pyrifera_M_contig88675.20591.1 vs. uniprot
Match: A0A1J4QGR4_9GAMM (Dioxygenase n=1 Tax=Oceanisphaera psychrotolerans TaxID=1414654 RepID=A0A1J4QGR4_9GAMM)

HSP 1 Score: 130 bits (327), Expect = 4.330e-35
Identity = 57/108 (52.78%), Postives = 77/108 (71.30%), Query Frame = 1
Query:   28 EWYRTFTGQWPKPSAIVVISAHWEERGSICVTAGAKHSLFYDYYGFPAHTYELGYDCPGEPALANRIVRMLQADGIPAKGNTARGYDHGGFSPLKLMYPDADIPVVQV 351
            +W +   G+  +P AI+++SAHWE      +TAGA+  L YDYYGFP  +YE+ Y CPGEPALA ++ + L A G+PA+ +  RG+DHG F PLKLMYP+ADIP VQ+
Sbjct:   30 QWLQDMAGRLRRPEAILIVSAHWEAAVPT-ITAGARPGLIYDYYGFPPESYEIQYPCPGEPALAQQVQQALMAAGMPARLDAERGFDHGVFVPLKLMYPEADIPCVQL 136          
BLAST of mRNA_M-pyrifera_M_contig88675.20591.1 vs. uniprot
Match: A0A1R4B5L6_9VIBR (LigB family dioxygenase n=1 Tax=Vibrio palustris TaxID=1918946 RepID=A0A1R4B5L6_9VIBR)

HSP 1 Score: 130 bits (326), Expect = 5.820e-35
Identity = 63/105 (60.00%), Postives = 75/105 (71.43%), Query Frame = 1
Query:   37 RTFTGQWPKPSAIVVISAHWEERGSICVTAGAKHSLFYDYYGFPAHTYELGYDCPGEPALANRIVRMLQADGIPAKGNTARGYDHGGFSPLKLMYPDADIPVVQV 351
            +   G+  KPSAI+VISAHWEE+    +TAGA  SL YDYYGFPA TYEL Y  PGEP LA  I   L++ G+ AK +T RG+DHG F PL LMYPDA IPVV++
Sbjct:   34 QALAGRLAKPSAILVISAHWEEKVPT-ITAGAHPSLIYDYYGFPAETYELTYPAPGEPKLAQEIYDCLKSSGVDAKLDTQRGWDHGVFVPLMLMYPDASIPVVEL 137          
BLAST of mRNA_M-pyrifera_M_contig88675.20591.1 vs. uniprot
Match: UPI001EF8DF4C (dioxygenase n=1 Tax=Oceanisphaera litoralis TaxID=225144 RepID=UPI001EF8DF4C)

HSP 1 Score: 129 bits (325), Expect = 8.620e-35
Identity = 61/101 (60.40%), Postives = 74/101 (73.27%), Query Frame = 1
Query:   49 GQWPKPSAIVVISAHWEERGSICVTAGAKHSLFYDYYGFPAHTYELGYDCPGEPALANRIVRMLQADGIPAKGNTARGYDHGGFSPLKLMYPDADIPVVQV 351
            G   KPSAI+VISAHWEE     +T+GAK  L YDYYGFP  +YE+ Y CPGEPALA +I + L+  G+PA+ +  RG+DHG F PLKLMYP ADIP VQ+
Sbjct:   37 GTLRKPSAILVISAHWEESVPT-ITSGAKPPLIYDYYGFPPESYEITYPCPGEPALARQIQQALEQAGLPARLDAQRGFDHGHFVPLKLMYPQADIPCVQL 136          
BLAST of mRNA_M-pyrifera_M_contig88675.20591.1 vs. uniprot
Match: A0A7V1LJY5_9BACT (Dioxygenase n=1 Tax=Caldithrix abyssi TaxID=187145 RepID=A0A7V1LJY5_9BACT)

HSP 1 Score: 129 bits (324), Expect = 1.190e-34
Identity = 60/105 (57.14%), Postives = 74/105 (70.48%), Query Frame = 1
Query:   37 RTFTGQWPKPSAIVVISAHWEERGSICVTAGAKHSLFYDYYGFPAHTYELGYDCPGEPALANRIVRMLQADGIPAKGNTARGYDHGGFSPLKLMYPDADIPVVQV 351
            R   G   KPSAI+VISAHWEE  +  VT+ A+  L YDY GFP  +Y + Y CPGEPALA + +R L++ GI A+ N  RG+DHG F PLK+MYP ADIPVVQ+
Sbjct:   32 RHIAGSMKKPSAILVISAHWEEE-TATVTSAARPPLIYDYNGFPEESYHIRYPCPGEPALAEKTLRALRSSGIAARANEQRGFDHGVFVPLKIMYPQADIPVVQL 135          
BLAST of mRNA_M-pyrifera_M_contig88675.20591.1 vs. uniprot
Match: UPI001247FA37 (dioxygenase n=1 Tax=Marinobacter TaxID=2742 RepID=UPI001247FA37)

HSP 1 Score: 129 bits (324), Expect = 1.190e-34
Identity = 60/97 (61.86%), Postives = 71/97 (73.20%), Query Frame = 1
Query:   61 KPSAIVVISAHWEERGSICVTAGAKHSLFYDYYGFPAHTYELGYDCPGEPALANRIVRMLQADGIPAKGNTARGYDHGGFSPLKLMYPDADIPVVQV 351
            KPSAI+VISAHWEE     +TAGAK  L YDYYGFP   YE+ Y CPGEPALA ++ + L   GIP + +  RG+DHG F PLKLMYPDADIP +Q+
Sbjct:   41 KPSAILVISAHWEESVPT-ITAGAKPPLIYDYYGFPPEAYEIEYPCPGEPALAQQVYQALDQAGIPVRLDEQRGFDHGLFVPLKLMYPDADIPCIQL 136          
BLAST of mRNA_M-pyrifera_M_contig88675.20591.1 vs. uniprot
Match: UPI001F5DC9F6 (dioxygenase n=1 Tax=Rhodocyclus purpureus TaxID=1067 RepID=UPI001F5DC9F6)

HSP 1 Score: 128 bits (322), Expect = 1.390e-34
Identity = 61/97 (62.89%), Postives = 73/97 (75.26%), Query Frame = 1
Query:   61 KPSAIVVISAHWEERGSICVTAGAKHSLFYDYYGFPAHTYELGYDCPGEPALANRIVRMLQADGIPAKGNTARGYDHGGFSPLKLMYPDADIPVVQV 351
            +PSAIVV+SAHW E  +  VT+G +  LFYDYYGFP HTYEL Y  PGEP LA RI  +L    +PA+ + ARG+DHG F PLKLM+PDADIPVVQ+
Sbjct:   26 RPSAIVVVSAHWLEP-AFSVTSGLQPELFYDYYGFPPHTYELRYPAPGEPQLAARIADLLGQARLPAQQDAARGFDHGMFIPLKLMFPDADIPVVQL 121          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig88675.20591.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LRX5_ECTSI1.120e-5265.81LigB domain-containing protein n=2 Tax=Ectocarpus ... [more]
H2FST0_OCESG1.060e-3560.00Extradiol ring-cleavage dioxygenase III subunit B ... [more]
A0A2E7E826_9GAMM2.860e-3561.39Dioxygenase n=1 Tax=Oceanospirillaceae bacterium T... [more]
A0A2M7G2W7_9BACT3.750e-3553.21Dioxygenase n=1 Tax=bacterium (Candidatus Blackall... [more]
A0A1J4QGR4_9GAMM4.330e-3552.78Dioxygenase n=1 Tax=Oceanisphaera psychrotolerans ... [more]
A0A1R4B5L6_9VIBR5.820e-3560.00LigB family dioxygenase n=1 Tax=Vibrio palustris T... [more]
UPI001EF8DF4C8.620e-3560.40dioxygenase n=1 Tax=Oceanisphaera litoralis TaxID=... [more]
A0A7V1LJY5_9BACT1.190e-3457.14Dioxygenase n=1 Tax=Caldithrix abyssi TaxID=187145... [more]
UPI001247FA371.190e-3461.86dioxygenase n=1 Tax=Marinobacter TaxID=2742 RepID=... [more]
UPI001F5DC9F61.390e-3462.89dioxygenase n=1 Tax=Rhodocyclus purpureus TaxID=10... [more]

Pages

back to top
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig88675contigM-pyrifera_M_contig88675:10..360 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score171.4
Seed ortholog evalue2.5e-40
Seed eggNOG ortholog2880.D8LRX5
KEGG rclassRC00387
KEGG koko:K15777
KEGG ReactionR08836
KEGG Pathwayko00965,map00965
Hectar predicted targeting categoryother localisation
EggNOG free text desc.ferrous iron binding
EggNOG OGs2QS66@2759,COG3384@1
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size351
Cds size348
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816797.1192834-CDS-M-pyrifera_M_contig88675:9..3571622816797.1192834-CDS-M-pyrifera_M_contig88675:9..357Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig88675 10..357 -
1692278184.1962817-CDS-M-pyrifera_M_contig88675:9..3571692278184.1962817-CDS-M-pyrifera_M_contig88675:9..357Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig88675 10..357 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816797.1334124-UTR-M-pyrifera_M_contig88675:357..3601622816797.1334124-UTR-M-pyrifera_M_contig88675:357..360Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig88675 358..360 -
1692278184.2083206-UTR-M-pyrifera_M_contig88675:357..3601692278184.2083206-UTR-M-pyrifera_M_contig88675:357..360Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig88675 358..360 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig88675.20591.1prot_M-pyrifera_M_contig88675.20591.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig88675 10..357 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig88675.20591.1

>prot_M-pyrifera_M_contig88675.20591.1 ID=prot_M-pyrifera_M_contig88675.20591.1|Name=mRNA_M-pyrifera_M_contig88675.20591.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=116bp
MNADSPAAEWYRTFTGQWPKPSAIVVISAHWEERGSICVTAGAKHSLFYD
YYGFPAHTYELGYDCPGEPALANRIVRMLQADGIPAKGNTARGYDHGGFS
PLKLMYPDADIPVVQV
back to top

mRNA from alignment at M-pyrifera_M_contig88675:10..360-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig88675.20591.1 ID=mRNA_M-pyrifera_M_contig88675.20591.1|Name=mRNA_M-pyrifera_M_contig88675.20591.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=351bp|location=Sequence derived from alignment at M-pyrifera_M_contig88675:10..360- (Macrocystis pyrifera P11B4 male)
CAGATGAACGCTGACAGCCCGGCGGCAGAGTGGTACCGCACCTTCACCGG GCAGTGGCCCAAACCCTCAGCCATCGTCGTCATTTCCGCCCACTGGGAAG AGCGTGGTTCCATCTGCGTCACGGCAGGCGCCAAGCACTCCCTGTTCTAC GACTACTACGGCTTCCCGGCGCACACGTACGAGCTGGGGTACGACTGCCC CGGCGAGCCCGCCCTGGCGAACCGCATCGTTCGAATGCTCCAGGCGGACG GGATCCCGGCGAAAGGGAACACTGCGCGGGGATACGACCACGGTGGGTTC AGCCCGCTGAAGCTCATGTATCCCGATGCCGACATCCCGGTGGTGCAGGT G
back to top

Coding sequence (CDS) from alignment at M-pyrifera_M_contig88675:10..360-

>mRNA_M-pyrifera_M_contig88675.20591.1 ID=mRNA_M-pyrifera_M_contig88675.20591.1|Name=mRNA_M-pyrifera_M_contig88675.20591.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=696bp|location=Sequence derived from alignment at M-pyrifera_M_contig88675:10..360- (Macrocystis pyrifera P11B4 male)
ATGAACGCTGACAGCCCGGCGGCAGAGTGGTACCGCACCTTCACCGGGCA
GTGGCCCAAACCCTCAGCCATCGTCGTCATTTCCGCCCACTGGGAAGAGC
GTGGTTCCATCTGCGTCACGGCAGGCGCCAAGCACTCCCTGTTCTACGAC
TACTACGGCTTCCCGGCGCACACGTACGAGCTGGGGTACGACTGCCCCGG
CGAGCCCGCCCTGGCGAACCGCATCGTTCGAATGCTCCAGGCGGACGGGA
TCCCGGCGAAAGGGAACACTGCGCGGGGATACGACCACGGTGGGTTCAGC
CCGCTGAAGCTCATGTATCCCGATGCCGACATCCCGGTGGTGCAGGTGAT
GAACGCTGACAGCCCGGCGGCAGAGTGGTACCGCACCTTCACCGGGCAGT
GGCCCAAACCCTCAGCCATCGTCGTCATTTCCGCCCACTGGGAAGAGCGT
GGTTCCATCTGCGTCACGGCAGGCGCCAAGCACTCCCTGTTCTACGACTA
CTACGGCTTCCCGGCGCACACGTACGAGCTGGGGTACGACTGCCCCGGCG
AGCCCGCCCTGGCGAACCGCATCGTTCGAATGCTCCAGGCGGACGGGATC
CCGGCGAAAGGGAACACTGCGCGGGGATACGACCACGGTGGGTTCAGCCC
GCTGAAGCTCATGTATCCCGATGCCGACATCCCGGTGGTGCAGGTG
back to top