mRNA_M-pyrifera_M_contig8864.20581.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig8864.20581.1
Unique NamemRNA_M-pyrifera_M_contig8864.20581.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig8864.20581.1 vs. uniprot
Match: D7G276_ECTSI (Mitochondrial import inner membrane translocase subunit TIM23 homolog n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G276_ECTSI)

HSP 1 Score: 213 bits (541), Expect = 2.590e-68
Identity = 101/115 (87.83%), Postives = 106/115 (92.17%), Query Frame = 1 
Query:    1 ESGGPQDPYSDASAKYGPLKLPQLQNVDLGKMYGGAAQGKEPDYLDYNIKGRSFFERTPYNAGAFYLAGIFGGGAMGVREGFAKAPNRRSRVLLNSVMNHAGKKGSLYGNTFAVL 345
            ESGG QDPYSDASAKYGPLKLP+LQNVDL KMYGGAAQGKEP+YLDYNIKGR F+ER PYNAGA Y+ GI GGGA GVREGFAKAPNRRSRVLLNS+MNHAGKKGS YGNTFAVL
Sbjct:    8 ESGGVQDPYSDASAKYGPLKLPELQNVDLSKMYGGAAQGKEPEYLDYNIKGRGFWERMPYNAGALYITGILGGGAAGVREGFAKAPNRRSRVLLNSIMNHAGKKGSFYGNTFAVL 122
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BLAST of mRNA_M-pyrifera_M_contig8864.20581.1 vs. uniprot
Match: A0A835Z638_9STRA (Mitochondrial import inner membrane translocase subunit TIM23 n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z638_9STRA)

HSP 1 Score: 125 bits (314), Expect = 7.310e-34
Identity = 66/118 (55.93%), Postives = 81/118 (68.64%), Query Frame = 1 
Query:   10 GPQDPYSDASAKYGPLKLPQLQ--NVDLGK---MYGGAAQGK-EPDYLDYNIKGRSFFERTPYNAGAFYLAGIFGGGAMGVREGFAKAPNRRSRVLLNSVMNHAGKKGSLYGNTFAVL 345
            G  DPY+D  AK+G L+LP+L   ++DLG    MYG    G  EPDYLDYNIKGR  FE+T YN G  YLAGI GGG  GV EG   AP+ R+R+ +N+++N AGK+GS  GNT AVL
Sbjct:   11 GDNDPYTDPGAKFGRLELPKLNTSSIDLGSLAGMYGVQGGGAYEPDYLDYNIKGRDTFEKTTYNTGMCYLAGIIGGGGYGVLEGARTAPSSRARIRVNAILNAAGKRGSRAGNTLAVL 128
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BLAST of mRNA_M-pyrifera_M_contig8864.20581.1 vs. uniprot
Match: A0A7S1VVP8_9STRA (Hypothetical protein n=2 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1VVP8_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 7.110e-20
Identity = 44/98 (44.90%), Postives = 59/98 (60.20%), Query Frame = 1 
Query:   52 PLKLPQLQNVDLGKMYGGAAQGKEPDYLDYNIKGRSFFERTPYNAGAFYLAGIFGGGAMGVREGFAKAPNRRSRVLLNSVMNHAGKKGSLYGNTFAVL 345
            PL      N+ LG +       ++PDYLDY+ KGR        NAG  YL G F GGA G+REGF  +P++R RV LNS++NH G+ GS +GN+  V+
Sbjct:   18 PLPDFSTANIQLGTVAPALGVPQQPDYLDYDQKGRGIVVTMFANAGMSYLLGTFAGGAYGLREGFIHSPSQRFRVKLNSILNHCGRHGSRWGNSLGVV 115
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BLAST of mRNA_M-pyrifera_M_contig8864.20581.1 vs. uniprot
Match: A0A6U5ID74_9STRA (Hypothetical protein n=2 Tax=Corethron hystrix TaxID=216773 RepID=A0A6U5ID74_9STRA)

HSP 1 Score: 89.4 bits (220), Expect = 7.600e-20
Identity = 51/105 (48.57%), Postives = 58/105 (55.24%), Query Frame = 1 
Query:   49 GPLKLPQLQ---NVDLGKM---YGGAAQGKEPDYLDYNIKGRSFFERTPYNAGAFYLAGIFGGGAMGVREGFAKAPNRRSRVLLNSVMNHAGKKGSLYGNTFAVL 345
            GP   P L    N+ LG +    G      EPDYLDYN+KGR   E+   N GA Y+   F GGAMG+R G A  PN R RV LNSV+NH  K GS  GN   VL
Sbjct:   14 GPPLPPSLMGGANIQLGTVAPALGTIGYAPEPDYLDYNLKGRGVSEKMFANTGASYVIATFIGGAMGLRSGLAATPNSRMRVKLNSVLNHCSKIGSKSGNAVGVL 118
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BLAST of mRNA_M-pyrifera_M_contig8864.20581.1 vs. uniprot
Match: A0A7S2QVE9_9STRA (Hypothetical protein n=1 Tax=Triparma pacifica TaxID=91992 RepID=A0A7S2QVE9_9STRA)

HSP 1 Score: 84.7 bits (208), Expect = 3.680e-18
Identity = 41/93 (44.09%), Postives = 59/93 (63.44%), Query Frame = 1 
Query:   52 PLKLPQLQNVDLGKMYGGAAQGKEPDYLDYNIKGRSFFERTPYNAGAFYLAGIFGGGAMGVREGFAKAPNRRSRVLLNSVMNHAGKKGSLYGN 330
            P  LP L  +DL ++ GG     EP+YLDY+IKGR +++RT  + G  YL G   GG  GV+ G +  PN R +V +N+++NH+G+ GS  GN
Sbjct:    8 PPTLPSLDGIDLRRVAGGITS--EPEYLDYDIKGRGWWDRTVSSTGWGYLIGTGVGGIYGVKTGMSSVPNSRFKVKVNALLNHSGRYGSRVGN 98
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BLAST of mRNA_M-pyrifera_M_contig8864.20581.1 vs. uniprot
Match: A0A7S2WJK1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WJK1_9STRA)

HSP 1 Score: 82.0 bits (201), Expect = 8.660e-18
Identity = 43/109 (39.45%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:   19 DPYSDASAKYGPLKLPQLQNVDLGKMYGGAAQGKEPDYLDYNIKGRSFFERTPYNAGAFYLAGIFGGGAMGVREGFAKAPNRRSRVLLNSVMNHAGKKGSLYGNTFAVL 345
            +P +++ A   P  +P L    LG ++G      EPDYL+Y++KGRS+FE+  +N G  Y+ G   G   G   G A AP+ + RV +N+++N AGK GS  GN   VL
Sbjct:    3 EPMAESEA---PAPIPHLDAPALGGIFGRPGVD-EPDYLEYDLKGRSYFEKLFFNTGLSYMIGSASGSLYGATSGLANAPSSKFRVKVNALLNGAGKYGSRGGNALGVL 107
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BLAST of mRNA_M-pyrifera_M_contig8864.20581.1 vs. uniprot
Match: A0A7S1XNQ0_9STRA (Hypothetical protein n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1XNQ0_9STRA)

HSP 1 Score: 83.6 bits (205), Expect = 1.170e-17
Identity = 43/98 (43.88%), Postives = 58/98 (59.18%), Query Frame = 1 
Query:   58 KLPQLQNVDLG---KMYGGAAQGK--EPDYLDYNIKGRSFFERTPYNAGAFYLAGIFGGGAMGVREGFAKAPNRRSRVLLNSVMNHAGKKGSLYGNTF 336
            +LP +  +D      + GG + G   EP+YLDY IK R   E      G  YLAG+ GGG +G+ EG   +P+ R R+L NS+MN+AGK+GS  GN F
Sbjct:   12 ELPPIPGIDFTLKQPIGGGLSTGSRYEPEYLDYTIKSRGPLENVFSYCGTAYLAGVIGGGGVGLMEGVRSSPSSRFRILFNSIMNNAGKRGSRAGNAF 109
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BLAST of mRNA_M-pyrifera_M_contig8864.20581.1 vs. uniprot
Match: A0A7S2S630_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2S630_9STRA)

HSP 1 Score: 82.0 bits (201), Expect = 3.900e-17
Identity = 43/109 (39.45%), Postives = 63/109 (57.80%), Query Frame = 1 
Query:   19 DPYSDASAKYGPLKLPQLQNVDLGKMYGGAAQGKEPDYLDYNIKGRSFFERTPYNAGAFYLAGIFGGGAMGVREGFAKAPNRRSRVLLNSVMNHAGKKGSLYGNTFAVL 345
            +P +++ A   P  +P L    LG ++G      EPDYL+Y++KGRS+FE+  +N G  Y+ G   G   G   G A AP+ + RV +N+++N AGK GS  GN   VL
Sbjct:    3 EPMAESEA---PAPIPHLDAPALGGIFGRPGVD-EPDYLEYDLKGRSYFEKLFFNTGLSYMIGSASGSLYGATSGLANAPSSKFRVKVNALLNGAGKYGSRGGNALGVL 107
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BLAST of mRNA_M-pyrifera_M_contig8864.20581.1 vs. uniprot
Match: A0A4D9D4E0_9STRA (Uncharacterized protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9D4E0_9STRA)

HSP 1 Score: 81.6 bits (200), Expect = 6.360e-17
Identity = 47/92 (51.09%), Postives = 59/92 (64.13%), Query Frame = 1 
Query:   76 NVDL---GKMYGGAAQGKEPDYLDYNIKGRSFFERTPYNAGAFYLAGIFGGGAMGVREGFAKAPNRRSRVLLNSVMNHAGKKGSLYGNTFAV 342
            NVDL     +YG A++ +EPDYLDY+IKGR   ER    +G+ YL GI  GG +G R G+  AP+ R  + LNSVMN AGK GS +GN   V
Sbjct:   27 NVDLRTVAPLYGVASE-EEPDYLDYDIKGRGTVERMFLYSGSSYLTGILTGGILGGRTGWKTAPSPRFWLRLNSVMNGAGKLGSKWGNNAGV 117
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BLAST of mRNA_M-pyrifera_M_contig8864.20581.1 vs. uniprot
Match: A0A7S2A828_TRICV (Hypothetical protein n=3 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S2A828_TRICV)

HSP 1 Score: 83.2 bits (204), Expect = 1.130e-16
Identity = 40/75 (53.33%), Postives = 48/75 (64.00%), Query Frame = 1 
Query:  121 EPDYLDYNIKGRSFFERTPYNAGAFYLAGIFGGGAMGVREGFAKAPNRRSRVLLNSVMNHAGKKGSLYGNTFAVL 345
            +PDYLDY+ KGR        NAG  YL GIFGGG  G+REG  + P+ R +V LNSV+NH G+ GS  GN   VL
Sbjct:  161 QPDYLDYDTKGRGVVVTMFANAGVSYLMGIFGGGLYGLREGLTQTPSSRFKVKLNSVLNHCGRHGSRAGNVLGVL 235
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig8864.20581.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 22
Match NameE-valueIdentityDescription
D7G276_ECTSI2.590e-6887.83Mitochondrial import inner membrane translocase su... [more]
A0A835Z638_9STRA7.310e-3455.93Mitochondrial import inner membrane translocase su... [more]
A0A7S1VVP8_9STRA7.110e-2044.90Hypothetical protein n=2 Tax=Grammatophora oceanic... [more]
A0A6U5ID74_9STRA7.600e-2048.57Hypothetical protein n=2 Tax=Corethron hystrix Tax... [more]
A0A7S2QVE9_9STRA3.680e-1844.09Hypothetical protein n=1 Tax=Triparma pacifica Tax... [more]
A0A7S2WJK1_9STRA8.660e-1839.45Hypothetical protein (Fragment) n=1 Tax=Rhizochrom... [more]
A0A7S1XNQ0_9STRA1.170e-1743.88Hypothetical protein n=1 Tax=Phaeomonas parva TaxI... [more]
A0A7S2S630_9STRA3.900e-1739.45Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A4D9D4E0_9STRA6.360e-1751.09Uncharacterized protein n=1 Tax=Nannochloropsis sa... [more]
A0A7S2A828_TRICV1.130e-1653.33Hypothetical protein n=3 Tax=Trieres chinensis Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig8864contigM-pyrifera_M_contig8864:96..1638 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score213.8
Seed ortholog evalue4.3e-53
Seed eggNOG ortholog2880.D7G276
Preferred nameTIMM23
KEGG rclassRC00034
KEGG koko:K00430,ko:K17794
KEGG TC3.A.8.1
KEGG ReactionR00602,R02596,R03919,R04007,R07443
KEGG Pathwayko00940,ko01100,ko01110,ko04212,map00940,map01100,map01110,map04212
Hectar predicted targeting categoryother localisation
GOsGO:0001101,GO:0003674,GO:0005048,GO:0005215,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005744,GO:0005758,GO:0006605,GO:0006626,GO:0006810,GO:0006839,GO:0006886,GO:0006996,GO:0007005,GO:0007006,GO:0007007,GO:0008104,GO:0008150,GO:0008320,GO:0008565,GO:0009719,GO:0009725,GO:0009893,GO:0009987,GO:0010033,GO:0010243,GO:0010468,GO:0010604,GO:0010628,GO:0010954,GO:0014070,GO:0015031,GO:0015399,GO:0015405,GO:0015450,GO:0015833,GO:0016020,GO:0016021,GO:0016043,GO:0017038,GO:0019222,GO:0019866,GO:0019899,GO:0022804,GO:0022857,GO:0022884,GO:0030150,GO:0030162,GO:0030943,GO:0031090,GO:0031224,GO:0031300,GO:0031301,GO:0031304,GO:0031305,GO:0031323,GO:0031325,GO:0031966,GO:0031967,GO:0031970,GO:0031974,GO:0031975,GO:0032268,GO:0032270,GO:0032592,GO:0032991,GO:0033036,GO:0033218,GO:0033365,GO:0034613,GO:0042221,GO:0042277,GO:0042719,GO:0042886,GO:0042887,GO:0043200,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044422,GO:0044424,GO:0044425,GO:0044429,GO:0044444,GO:0044446,GO:0044455,GO:0044464,GO:0044743,GO:0045039,GO:0045184,GO:0045862,GO:0046907,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051173,GO:0051179,GO:0051234,GO:0051246,GO:0051247,GO:0051641,GO:0051649,GO:0055085,GO:0060255,GO:0061024,GO:0065002,GO:0065007,GO:0070013,GO:0070585,GO:0070613,GO:0070727,GO:0071702,GO:0071705,GO:0071806,GO:0071840,GO:0072594,GO:0072655,GO:0072657,GO:0080090,GO:0090150,GO:0090151,GO:0097066,GO:0098573,GO:0098796,GO:0098798,GO:0098800,GO:1901698,GO:1901700,GO:1903317,GO:1903319,GO:1904680,GO:1905242,GO:1990351,GO:1990542
EggNOG free text desc.protein import into mitochondrial matrix
EggNOG OGsCOG5596@1,KOG3324@2759
Ec32 ortholog descriptionMitochondrial import inner membrane translocase subunit TIM23 homolog
Ec32 orthologEc-16_002190.1
EC1.11.1.7
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko02000,ko03029
Exons3
Model size345
Cds size345
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig8864.20581.1prot_M-pyrifera_M_contig8864.20581.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig8864 96..1638 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816796.7193534-CDS-M-pyrifera_M_contig8864:95..2351622816796.7193534-CDS-M-pyrifera_M_contig8864:95..235Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8864 96..235 -
1692278183.9287217-CDS-M-pyrifera_M_contig8864:95..2351692278183.9287217-CDS-M-pyrifera_M_contig8864:95..235Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8864 96..235 -
1622816796.7307448-CDS-M-pyrifera_M_contig8864:979..10701622816796.7307448-CDS-M-pyrifera_M_contig8864:979..1070Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8864 980..1070 -
1692278183.9483018-CDS-M-pyrifera_M_contig8864:979..10701692278183.9483018-CDS-M-pyrifera_M_contig8864:979..1070Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8864 980..1070 -
1622816796.7429802-CDS-M-pyrifera_M_contig8864:1524..16381622816796.7429802-CDS-M-pyrifera_M_contig8864:1524..1638Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8864 1525..1638 -
1692278183.9640336-CDS-M-pyrifera_M_contig8864:1524..16381692278183.9640336-CDS-M-pyrifera_M_contig8864:1524..1638Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8864 1525..1638 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig8864.20581.1

>prot_M-pyrifera_M_contig8864.20581.1 ID=prot_M-pyrifera_M_contig8864.20581.1|Name=mRNA_M-pyrifera_M_contig8864.20581.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=115bp
ESGGPQDPYSDASAKYGPLKLPQLQNVDLGKMYGGAAQGKEPDYLDYNIK
GRSFFERTPYNAGAFYLAGIFGGGAMGVREGFAKAPNRRSRVLLNSVMNH
AGKKGSLYGNTFAVL
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mRNA from alignment at M-pyrifera_M_contig8864:96..1638-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig8864.20581.1 ID=mRNA_M-pyrifera_M_contig8864.20581.1|Name=mRNA_M-pyrifera_M_contig8864.20581.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=1543bp|location=Sequence derived from alignment at M-pyrifera_M_contig8864:96..1638- (Macrocystis pyrifera P11B4 male)
GAGAGCGGGGGGCCGCAAGACCCTTACAGCGATGCTAGCGCGAAGTACGG
ACCGCTTAAGCTGCCGCAGTTACAAAATGTCGATCTGGGCAAGATGTATG
GTGGGGCCGCGCAGGTGAGGAACGGGTGGAGAGAGGGGCGGGCAGGGGTG
GGGGGTGGGGAGGGGAGGGAAAGGGGGGTGGGGGTGGGGGTGGAGTAGAT
GAACGGAGAGGGGGAGGGGGGGTAGCAGGGAGAGAGGGGAAGGGGGGGGG
GAGGAGGGGGAAGTGGAGTTGAGGAGAGGGGAGGGAAGGGACACGGGGTC
AGGAGGCGAAAGCGGCATGTGCCTTTGCCTGCCTGCCTTGCTTGTTGCTT
GCTGCGCCTCGTAGATGCCTGCGGGTAGAGAGGGGAAGGGGACGGGAAGG
AAGTGGAGGGAGGGCGAGGGGGTGGCATATAGCGAAGGGGGAAGGGGAGG
GGGGGGAGGTCAGGAGACGATGGTAACGTGTGCCTTGCCTGCCTCTGCTT
GCTTGTCTGCGCCTTCTAATGTCTCCTTGCTGCCCGCCTGCTTGCTTGCG
TTCTGCGCATCCTAAAAGGGAAAGGAGCCAGACTACTTGGATTACAACAT
CAAGGGGAGGAGCTTTTTCGAGCGCACGCCGTACAACGCCGGAGCGTTTT
ACCTTGCCGGTGAGGGACCGAAAATACTCAAAACATTGTTTTTCCGACAG
TTTCATTGTTTTTCATGGGCGGTGGACTCTGTACTGTATCGTACGGTACT
ATTAATACTGTGCCGTCCTTAGCTTTTCCGTAGCGTGCCGTACCATACCA
TGCCGCACCGCAGCGTACCGTATCTCACAGTACCGCACTGCACCGCATTG
CGCCAAGCCAGACCAAACCGAACCAAACCGAACCAAACCGAACCAAACCA
AACCAAACCAAACCAAGCCAAACCAAACCAAACCAAACCAAGCCAAACCA
AACCAAACCAACCCAACCCAACCCAAACCAACCCAAACCAAACCAAACCA
AACCAAACCAAACCAGACCAAACCAAACCAAGCCAAACCAAACCAAATCA
CACCAAACCAAACCAAGCCAAACCAAGTCAAGCCTGACTAAACCTCCACC
ACATCCCGTCTGGCTAGTCCCCGCACTACCTGTAACGTGCGCATTTGTCG
GTCGATCGATCGATCGATCGATCGTCAGGCTTGTTGTCGTTGATGTTGCT
ATTACGGGGGTCCATAGTTAACGGGACCAAATTTTGTTGGTAAAAATAGG
CAAATATATAGGTTTTTATGTACACCATAGGTCCTGTTAACTATGGACCC
CCGTAATAGCGTTGCTGCTAACCTTGGTGGTGGTGCTTGCTGTTGTTGTT
TATTCGCCCTCTTCGTCATTGTGGCCTTTGTTCGATCGAACGGTTCCGCG
CAGGTATTTTTGGGGGTGGAGCAATGGGGGTACGGGAAGGCTTCGCCAAG
GCGCCCAACCGACGATCGAGGGTGCTGCTCAACAGCGTCATGAACCACGC
CGGAAAGAAGGGGTCTCTGTACGGCAACACCTTCGCCGTCCTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig8864:96..1638-

>mRNA_M-pyrifera_M_contig8864.20581.1 ID=mRNA_M-pyrifera_M_contig8864.20581.1|Name=mRNA_M-pyrifera_M_contig8864.20581.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=690bp|location=Sequence derived from alignment at M-pyrifera_M_contig8864:96..1638- (Macrocystis pyrifera P11B4 male)
GAGAGCGGGGGGCCGCAAGACCCTTACAGCGATGCTAGCGCGAAGTACGG
ACCGCTTAAGCTGCCGCAGTTACAAAATGTCGATCTGGGCAAGATGTATG
GTGGGGCCGCGCAGGAGAGCGGGGGGCCGCAAGACCCTTACAGCGATGCT
AGCGCGAAGTACGGACCGCTTAAGCTGCCGCAGTTACAAAATGTCGATCT
GGGCAAGATGTATGGTGGGGCCGCGCAGGGAAAGGAGCCAGACTACTTGG
ATTACAACATCAAGGGGAGGAGCTTTTTCGAGCGCACGCCGTACAACGCC
GGAGCGTTTTACCTTGCCGGGAAAGGAGCCAGACTACTTGGATTACAACA
TCAAGGGGAGGAGCTTTTTCGAGCGCACGCCGTACAACGCCGGAGCGTTT
TACCTTGCCGGTATTTTTGGGGGTGGAGCAATGGGGGTACGGGAAGGCTT
CGCCAAGGCGCCCAACCGACGATCGAGGGTGCTGCTCAACAGCGTCATGA
ACCACGCCGGAAAGAAGGGGTCTCTGTACGGCAACACCTTCGCCGTCCTC
GTATTTTTGGGGGTGGAGCAATGGGGGTACGGGAAGGCTTCGCCAAGGCG
CCCAACCGACGATCGAGGGTGCTGCTCAACAGCGTCATGAACCACGCCGG
AAAGAAGGGGTCTCTGTACGGCAACACCTTCGCCGTCCTC
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