mRNA_M-pyrifera_M_contig87808.20400.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig87808.20400.1
Unique NamemRNA_M-pyrifera_M_contig87808.20400.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig87808.20400.1 vs. uniprot
Match: D5C222_NITHN (D-alanine--D-alanine ligase domain protein n=2 Tax=Nitrosococcus TaxID=1227 RepID=D5C222_NITHN)

HSP 1 Score: 207 bits (527), Expect = 7.040e-63
Identity = 103/187 (55.08%), Postives = 134/187 (71.66%), Query Frame = 1
Query:    1 VKPNRGDGSVGITQASIVGDAATASATIAALRRDIPEVDLLVQEFLSGEEYGLGVIGNPAEGLAALPLMAIDYSALDPA-LPRLLDYSSKVDPDSPYWNDIRYPAATLDAQTQARMLEWCTTLFERLQLRDYARFDFRANAAGQIKLLEVNPNPAWCWDGKLAHMGELAHLSHSDVLARIIAAAERR 558
            +KPNR DGS+GIT+ S+V D   A A +  L  + P  DLL+QEFL GEEY +GV+GNPA G   LP++A+DYS L  + LP +L Y+SK+DP SPYW DI+Y  A+L    + R++++C  LFERL  RDYARFDFR +A G+IKLLEVNPNPAWCWDGKL  M   A   ++++L RI+ AA+ R
Sbjct:  162 IKPNRADGSLGITKDSLVHDEQQARAYLNKLAMEFPNWDLLIQEFLPGEEYSVGVLGNPASGFMILPILAVDYSKLVASRLPPILAYASKIDPQSPYWTDIKYCEASLKEDLRQRLVDYCLFLFERLGCRDYARFDFRTDAQGEIKLLEVNPNPAWCWDGKLNLMAGFAGYHYAELLERILHAAQVR 348          
BLAST of mRNA_M-pyrifera_M_contig87808.20400.1 vs. uniprot
Match: A0A327JGY6_9HYPH (D-alanine--D-alanine ligase n=3 Tax=Rhodobium orientis TaxID=34017 RepID=A0A327JGY6_9HYPH)

HSP 1 Score: 211 bits (537), Expect = 1.750e-61
Identity = 110/196 (56.12%), Postives = 135/196 (68.88%), Query Frame = 1
Query:    1 VKPNRGDGSVGITQASIVGDAATASATIAALRRDIPEVDLLVQEFLSGEEYGLGVIGNPAEGLAALPLMAIDYSALDPALPRLLDYSSKVDPDSPYWNDIRYPAATLDAQTQARMLEWCTTLFERLQLRDYARFDFRANAAGQIKLLEVNPNPAWCWDGKLAHMGELAHLSHSDVLARIIAAAERRIAADGERTSD 588
            +KPN GD S GIT+ ++V +     A +  LR   P+  +LVQEFLSG EY +G+IGNP  GL ALPL+ +DYS LDP LP++L Y SK  P+SPYW  IRY  ATL  Q QA +++    LFERL  RDYARFDFRA+AAG IKLLEVNPNP WCWDGK   M   A +S++D+L  II AAE R+  +G R SD
Sbjct:  445 LKPNFGDSSEGITRDAVVTNQQELLAYLDGLRALFPDNPVLVQEFLSGGEYSVGLIGNPDRGLRALPLLEVDYSGLDPELPQILCYESKWHPESPYWTRIRYREATLADQEQAMLIDHSMRLFERLGCRDYARFDFRADAAGTIKLLEVNPNPGWCWDGKQNLMAGFAGMSYADLLEAIIGAAEERLGLNGRRRSD 640          
BLAST of mRNA_M-pyrifera_M_contig87808.20400.1 vs. uniprot
Match: A0A1Y0BNZ9_9BACT (K774 n=1 Tax=uncultured bacterium TaxID=77133 RepID=A0A1Y0BNZ9_9BACT)

HSP 1 Score: 209 bits (533), Expect = 6.820e-61
Identity = 100/188 (53.19%), Postives = 133/188 (70.74%), Query Frame = 1
Query:    1 VKPNRGDGSVGITQASIVGDAATASATIAALRRDIPEVDLLVQEFLSGEEYGLGVIGNPAEGLAALPLMAIDYSALDPALPRLLDYSSKVDPDSPYWNDIRYPAATLDAQTQARMLEWCTTLFERLQLRDYARFDFRANAAGQIKLLEVNPNPAWCWDGKLAHMGELAHLSHSDVLARIIAAAERRIA 564
            +KP  GD S+GIT+ ++V + + A + ++ LRR +P   +LVQEFLSG EY +G+IGNP  G   LP++ +DY  L P LP++L Y SK DP SPYW  IRY  A+LD + +  M++W T LFERL+ RDY RFDFRA++AG++KLLEVNPNP WCWDGKL  M       ++D+L  II AA+RRIA
Sbjct:  446 IKPCMGDSSIGITKDAVVHNPSEAVSYLSELRRILPGRPVLVQEFLSGNEYSVGIIGNPGIGFTVLPILEVDYCGLPPELPKILGYESKWDPASPYWTQIRYREASLDDELRRSMIDWSTALFERLECRDYVRFDFRADSAGEVKLLEVNPNPGWCWDGKLNLMAGFGGYRYADMLRMIIEAAQRRIA 633          
BLAST of mRNA_M-pyrifera_M_contig87808.20400.1 vs. uniprot
Match: Q3J914_NITOC (ATP-grasp domain-containing protein n=5 Tax=Nitrosococcus TaxID=1227 RepID=Q3J914_NITOC)

HSP 1 Score: 201 bits (511), Expect = 2.000e-60
Identity = 101/189 (53.44%), Postives = 130/189 (68.78%), Query Frame = 1
Query:    1 VKPNRGDGSVGITQASIVGDAATASATIAALRRDIPEVDLLVQEFLSGEEYGLGVIGNPAEGLAALPLMAIDYSALDP-ALPRLLDYSSKVDPDSPYWNDIRYPAATLDAQTQARMLEWCTTLFERLQLRDYARFDFRANAAGQIKLLEVNPNPAWCWDGKLAHMGELAHLSHSDVLARIIAAAERRIA 564
            +KPNRGDGSVGIT+ S+V D   A A I  L  + P  DLL+QEFL G+EY +G++GNP+ G   LP++ +DYS L    LP +L Y+SKVD  SPYW DI+Y  A +D   + R++ +C  LFERL  RDYARFDFRA+  G++KLLEVNPNPAWCWDGKL  M   A   + ++L  I+ AA+ R A
Sbjct:  155 IKPNRGDGSVGITENSLVYDEPQAHAYINKLAMEFPNCDLLIQEFLPGDEYSVGLLGNPSSGFKILPILKVDYSRLAAHRLPPILAYASKVDSASPYWTDIQYAKAAMDEGLRQRLVGYCRLLFERLGCRDYARFDFRADVQGEVKLLEVNPNPAWCWDGKLNLMAGFAGYRYGELLEWILQAAQARYA 343          
BLAST of mRNA_M-pyrifera_M_contig87808.20400.1 vs. uniprot
Match: A0A7S6MBM1_9BACT (Methyltransferase domain-containing protein n=1 Tax=Phycisphaeraceae bacterium TaxID=2026777 RepID=A0A7S6MBM1_9BACT)

HSP 1 Score: 208 bits (529), Expect = 5.170e-60
Identity = 105/188 (55.85%), Postives = 130/188 (69.15%), Query Frame = 1
Query:    1 VKPNRGDGSVGITQASIVGDAATASATIAALRRDIPEVDLLVQEFLSGEEYGLGVIGNPAEGLAALPLMAIDYSALDPALPRLLDYSSKVDPDSPYWNDIRYPAATLDAQTQARMLEWCTTLFERLQLRDYARFDFRANAAGQIKLLEVNPNPAWCWDGKLAHMGELAHLSHSDVLARIIAAAERRIA 564
            VKPN GD S+GIT+ S+V         +  LR  +P+  +LVQEFLSG EY +GV+GNP   +  LP+M +DYS LDP LP++L Y SK  PDSPYW+ I+Y  A LD  T  +M++    LFERLQ RDYARFDFRA+A G  KLLEVNPNP WCWDGKL  M ELA L ++D+L  I+ AA+ RIA
Sbjct:  488 VKPNTGDSSIGITKNSVVSSPRELVQYLRTLREQLPDRAVLVQEFLSGTEYTVGVVGNPGLSMRILPVMEVDYSGLDPDLPKILGYESKWHPDSPYWSQIKYIPARLDEDTMRQMVDHSLLLFERLQCRDYARFDFRADANGVPKLLEVNPNPGWCWDGKLNLMAELAGLRYADLLKLILEAAQERIA 675          
BLAST of mRNA_M-pyrifera_M_contig87808.20400.1 vs. uniprot
Match: A0A1G2ZD60_9BACT (ATP-grasp domain-containing protein n=1 Tax=Planctomycetes bacterium RBG_13_63_9 TaxID=1801965 RepID=A0A1G2ZD60_9BACT)

HSP 1 Score: 202 bits (513), Expect = 7.190e-60
Identity = 97/188 (51.60%), Postives = 129/188 (68.62%), Query Frame = 1
Query:    1 VKPNRGDGSVGITQASIVGDAATASATIAALRRDIPEVDLLVQEFLSGEEYGLGVIGNPAEGLAALPLMAIDYSALDPALPRLLDYSSKVDPDSPYWNDIRYPAATLDAQTQARMLEWCTTLFERLQLRDYARFDFRANAAGQIKLLEVNPNPAWCWDGKLAHMGELAHLSHSDVLARIIAAAERRIA 564
            VKPN GD S+GITQ ++V         +  LR D+P   +L+QEFL  EE+ +G+IGNP  G   LP++ +DYS LDP LP +L Y SK +PDSPYW+ I Y  A +D + + ++++    LFERL+ RDYARFDFR +AAG+IKLLEVNPNP WCWDGKL +M   A L ++DVL  I+  A+ R+A
Sbjct:  218 VKPNYGDSSIGITQDAVVHTPEQLIGYLDKLRDDLPGRPVLIQEFLPNEEFSVGIIGNPGVGYNVLPVVQVDYSGLDPGLPWILGYESKWEPDSPYWSQINYHEAHIDEEAKRKLIDHSILLFERLECRDYARFDFRTDAAGRIKLLEVNPNPGWCWDGKLNYMAGFAGLRYADVLRSILMVAQERVA 405          
BLAST of mRNA_M-pyrifera_M_contig87808.20400.1 vs. uniprot
Match: A0A7X9FTH0_9DELT (D-alanine--D-alanine ligase n=1 Tax=SAR324 cluster bacterium TaxID=2024889 RepID=A0A7X9FTH0_9DELT)

HSP 1 Score: 200 bits (509), Expect = 9.040e-60
Identity = 95/187 (50.80%), Postives = 129/187 (68.98%), Query Frame = 1
Query:    1 VKPNRGDGSVGITQASIVGDAATASATIAALRRDIPEVDLLVQEFLSGEEYGLGVIGNPAEGLAALPLMAIDYSALDPALPRLLDYSSKVDPDSPYWNDIRYPAATLDAQTQARMLEWCTTLFERLQLRDYARFDFRANAAGQIKLLEVNPNPAWCWDGKLAHMGELAHLSHSDVLARIIAAAERRI 561
            VKPN GD S+GITQ ++V D+      +  L   +P   +L+QEFLSG EY +G+IGNP   +  LP + +DY  LDP LP++L Y SK DP+SPYW  I+Y  A +D +T+ ++ ++ + LFERL  RDYARFDFRA+A G++KLLEVNPNP WCWDGK   M   A + +S++L R++ AAE RI
Sbjct:  171 VKPNFGDNSIGITQNAVVRDSTELLEYLNKLWESMPNTPVLIQEFLSGPEYSIGIIGNPGLSMDYLPPLEVDYYGLDPKLPKILGYESKWDPNSPYWTQIKYKEAEIDEETRRKLYDYSSILFERLGCRDYARFDFRADAEGEVKLLEVNPNPGWCWDGKFNIMCGFAGMGYSEMLLRVLDAAEERI 357          
BLAST of mRNA_M-pyrifera_M_contig87808.20400.1 vs. uniprot
Match: A4BTL3_9GAMM (ATP-grasp domain-containing protein n=1 Tax=Nitrococcus mobilis Nb-231 TaxID=314278 RepID=A4BTL3_9GAMM)

HSP 1 Score: 196 bits (499), Expect = 1.080e-58
Identity = 97/187 (51.87%), Postives = 128/187 (68.45%), Query Frame = 1
Query:    1 VKPNRGDGSVGITQASIVGDAATASATIAALRRDIPEVDLLVQEFLSGEEYGLGVIGNPAEGLAALPLMAIDYSALDPALPRLLDYSSKVDPDSPYWNDIRYPAATLDAQTQARMLEWCTTLFERLQLRDYARFDFRANAAGQIKLLEVNPNPAWCWDGKLAHMGELAHLSHSDVLARIIAAAERRI 561
            +KPN  DGS GIT   +  DAA A   +  L  ++   + L+Q+FL+G EY +G+IGN ++G   LP + +DYS LDP LP +L Y+SK DPDSPYW  +R+  A +D  T+ R+ E+CT LF+RL LRDY RFDFRA A G+ +LLEVN NPAW WDGKL+ M   A  S+SD+L  I+AAA RR+
Sbjct:  155 IKPNFSDGSFGITADCVAHDAAEAERYLHWLAGELERPEALIQDFLTGTEYTVGLIGNASQGFTYLPPLEVDYSRLDPELPPILSYASKADPDSPYWQKLRFRRARIDEVTRGRLYEYCTLLFKRLGLRDYGRFDFRAGADGEPRLLEVNHNPAWAWDGKLSLMASYAGYSYSDLLRLILAAAMRRV 341          
BLAST of mRNA_M-pyrifera_M_contig87808.20400.1 vs. uniprot
Match: A0A2H0MPX3_9BACT (D-alanine--D-alanine ligase n=1 Tax=Nitrospinae bacterium CG11_big_fil_rev_8_21_14_0_20_45_15 TaxID=1974048 RepID=A0A2H0MPX3_9BACT)

HSP 1 Score: 203 bits (517), Expect = 1.320e-58
Identity = 99/187 (52.94%), Postives = 132/187 (70.59%), Query Frame = 1
Query:    1 VKPNRGDGSVGITQASIVGDAATASATIAALRRDIPEVDLLVQEFLSGEEYGLGVIGNPAEGLAALPLMAIDYSALDPALPRLLDYSSKVDPDSPYWNDIRYPAATLDAQTQARMLEWCTTLFERLQLRDYARFDFRANAAGQIKLLEVNPNPAWCWDGKLAHMGELAHLSHSDVLARIIAAAERRI 561
            +KPN GD S+GITQ ++V +A    + +  LR  +P + LL+QEFL G EY +G+IGN A+    LP++ +DYS L P LP++L Y SK DPDSPYW+DIRY  A LD +T  +++ + + LFERL+ RDYARFDFR +A G++KLLEVNPNP WCWDGKL  M   A   +SD+L  I+ AA+ RI
Sbjct:  456 IKPNCGDSSIGITQHAVVHNAMELMSYLDHLREILPGIPLLIQEFLEGTEYSVGLIGN-ADKFEVLPILEVDYSKLPPELPQVLSYESKWDPDSPYWSDIRYKEAVLDDETHRKLIHFSSRLFERLECRDYARFDFRTDATGRVKLLEVNPNPGWCWDGKLNLMAGFAGYDYSDLLKMILQAAKERI 641          
BLAST of mRNA_M-pyrifera_M_contig87808.20400.1 vs. uniprot
Match: A0A2D9F473_9PROT (D-alanine--D-alanine ligase n=1 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A2D9F473_9PROT)

HSP 1 Score: 202 bits (514), Expect = 3.220e-58
Identity = 103/191 (53.93%), Postives = 128/191 (67.02%), Query Frame = 1
Query:    1 VKPNRGDGSVGITQASIVGDAATASATIAALRRDIPEVDLLVQEFLSGEEYGLGVIGNPAEGLAALPLMAIDYSALDPALPRLLDYSSKVDPDSPYWNDIRYPAATLDAQTQARMLEWCTTLFERLQLRDYARFDFRANAAGQIKLLEVNPNPAWCWDGKLAHMGELAHLSHSDVLARIIAAAERRIAADG 573
            +KP +GD S+GITQ ++V       A +A +RR +P   +LVQEFL G EY + +IGNP  G  ALP++A+DYS LDP LP+LL Y SK  PDSPYW DI+Y  A LD  ++  M +   TLF+RL  RDYARFDFR +A G IKLLEVNPNP WCWDGK   M     L + D+L RII A + R AA+G
Sbjct:  439 IKPAQGDSSIGITQDAVVHTPEEMVAYLAEVRRTLPGRSVLVQEFLDGAEYSIALIGNPGSGFHALPVLAVDYSGLDPNLPKLLGYESKWQPDSPYWTDIQYRQADLDEDSRRAMFDASATLFQRLGCRDYARFDFRTDAQGAIKLLEVNPNPGWCWDGKFNLMAGFEGLEYPDLLRRIIEAGQAR-AAEG 628          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig87808.20400.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D5C222_NITHN7.040e-6355.08D-alanine--D-alanine ligase domain protein n=2 Tax... [more]
A0A327JGY6_9HYPH1.750e-6156.12D-alanine--D-alanine ligase n=3 Tax=Rhodobium orie... [more]
A0A1Y0BNZ9_9BACT6.820e-6153.19K774 n=1 Tax=uncultured bacterium TaxID=77133 RepI... [more]
Q3J914_NITOC2.000e-6053.44ATP-grasp domain-containing protein n=5 Tax=Nitros... [more]
A0A7S6MBM1_9BACT5.170e-6055.85Methyltransferase domain-containing protein n=1 Ta... [more]
A0A1G2ZD60_9BACT7.190e-6051.60ATP-grasp domain-containing protein n=1 Tax=Planct... [more]
A0A7X9FTH0_9DELT9.040e-6050.80D-alanine--D-alanine ligase n=1 Tax=SAR324 cluster... [more]
A4BTL3_9GAMM1.080e-5851.87ATP-grasp domain-containing protein n=1 Tax=Nitroc... [more]
A0A2H0MPX3_9BACT1.320e-5852.94D-alanine--D-alanine ligase n=1 Tax=Nitrospinae ba... [more]
A0A2D9F473_9PROT3.220e-5853.93D-alanine--D-alanine ligase n=1 Tax=Rhodospirillac... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig87808contigM-pyrifera_M_contig87808:6..602 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score210.7
Seed ortholog evalue6.3e-52
Seed eggNOG ortholog472759.Nhal_3587
KEGG rclassRC00064,RC00141
KEGG koko:K01921
KEGG ReactionR01150
KEGG Pathwayko00473,ko00550,ko01100,ko01502,map00473,map00550,map01100,map01502
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Belongs to the D-alanine--D-alanine ligase family
EggNOG OGs1N4F5@1224,1SKJ8@1236,1X0FH@135613,COG1181@1,COG1181@2
EC6.3.2.4
COG Functional cat.F
Best tax levelChromatiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01011
Exons1
Model size597
Cds size597
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816787.5043948-CDS-M-pyrifera_M_contig87808:5..6021622816787.5043948-CDS-M-pyrifera_M_contig87808:5..602Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87808 6..602 +
1692278178.4741232-CDS-M-pyrifera_M_contig87808:5..6021692278178.4741232-CDS-M-pyrifera_M_contig87808:5..602Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87808 6..602 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig87808.20400.1prot_M-pyrifera_M_contig87808.20400.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig87808 6..602 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig87808.20400.1

>prot_M-pyrifera_M_contig87808.20400.1 ID=prot_M-pyrifera_M_contig87808.20400.1|Name=mRNA_M-pyrifera_M_contig87808.20400.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=199bp
VKPNRGDGSVGITQASIVGDAATASATIAALRRDIPEVDLLVQEFLSGEE
YGLGVIGNPAEGLAALPLMAIDYSALDPALPRLLDYSSKVDPDSPYWNDI
RYPAATLDAQTQARMLEWCTTLFERLQLRDYARFDFRANAAGQIKLLEVN
PNPAWCWDGKLAHMGELAHLSHSDVLARIIAAAERRIAADGERTSDVR*
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mRNA from alignment at M-pyrifera_M_contig87808:6..602+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig87808.20400.1 ID=mRNA_M-pyrifera_M_contig87808.20400.1|Name=mRNA_M-pyrifera_M_contig87808.20400.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=597bp|location=Sequence derived from alignment at M-pyrifera_M_contig87808:6..602+ (Macrocystis pyrifera P11B4 male)
GTGAAACCAAACCGCGGCGACGGCAGCGTCGGGATCACCCAGGCATCAAT CGTGGGCGATGCGGCCACCGCGAGCGCCACGATTGCTGCATTACGGCGTG ACATCCCGGAGGTTGATCTGCTCGTACAGGAATTCCTCAGCGGTGAGGAG TACGGACTCGGCGTGATTGGAAATCCGGCTGAGGGCCTTGCCGCGCTGCC GCTCATGGCGATCGATTACAGCGCACTCGATCCCGCCCTGCCGCGCCTGC TCGATTACAGTTCGAAAGTCGATCCCGACTCGCCGTACTGGAACGATATC CGCTATCCGGCTGCGACGCTGGATGCACAAACGCAGGCACGCATGCTCGA ATGGTGCACGACGCTGTTCGAGCGACTGCAGCTGCGTGATTACGCGCGCT TCGACTTTCGCGCGAACGCGGCCGGACAAATCAAGTTGCTCGAGGTGAAT CCGAATCCGGCATGGTGCTGGGACGGCAAGCTCGCGCACATGGGCGAACT CGCGCACCTGAGCCACAGCGATGTGCTCGCGCGGATTATTGCCGCGGCCG AGCGGCGCATTGCCGCCGACGGCGAACGCACATCAGACGTGCGCTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig87808:6..602+

>mRNA_M-pyrifera_M_contig87808.20400.1 ID=mRNA_M-pyrifera_M_contig87808.20400.1|Name=mRNA_M-pyrifera_M_contig87808.20400.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1194bp|location=Sequence derived from alignment at M-pyrifera_M_contig87808:6..602+ (Macrocystis pyrifera P11B4 male)
GTGAAACCAAACCGCGGCGACGGCAGCGTCGGGATCACCCAGGCATCAAT
CGTGGGCGATGCGGCCACCGCGAGCGCCACGATTGCTGCATTACGGCGTG
ACATCCCGGAGGTTGATCTGCTCGTACAGGAATTCCTCAGCGGTGAGGAG
TACGGACTCGGCGTGATTGGAAATCCGGCTGAGGGCCTTGCCGCGCTGCC
GCTCATGGCGATCGATTACAGCGCACTCGATCCCGCCCTGCCGCGCCTGC
TCGATTACAGTTCGAAAGTCGATCCCGACTCGCCGTACTGGAACGATATC
CGCTATCCGGCTGCGACGCTGGATGCACAAACGCAGGCACGCATGCTCGA
ATGGTGCACGACGCTGTTCGAGCGACTGCAGCTGCGTGATTACGCGCGCT
TCGACTTTCGCGCGAACGCGGCCGGACAAATCAAGTTGCTCGAGGTGAAT
CCGAATCCGGCATGGTGCTGGGACGGCAAGCTCGCGCACATGGGCGAACT
CGCGCACCTGAGCCACAGCGATGTGCTCGCGCGGATTATTGCCGCGGCCG
AGCGGCGCATTGCCGCCGACGGCGAACGCACATCAGACGTGCGCTGAGTG
AAACCAAACCGCGGCGACGGCAGCGTCGGGATCACCCAGGCATCAATCGT
GGGCGATGCGGCCACCGCGAGCGCCACGATTGCTGCATTACGGCGTGACA
TCCCGGAGGTTGATCTGCTCGTACAGGAATTCCTCAGCGGTGAGGAGTAC
GGACTCGGCGTGATTGGAAATCCGGCTGAGGGCCTTGCCGCGCTGCCGCT
CATGGCGATCGATTACAGCGCACTCGATCCCGCCCTGCCGCGCCTGCTCG
ATTACAGTTCGAAAGTCGATCCCGACTCGCCGTACTGGAACGATATCCGC
TATCCGGCTGCGACGCTGGATGCACAAACGCAGGCACGCATGCTCGAATG
GTGCACGACGCTGTTCGAGCGACTGCAGCTGCGTGATTACGCGCGCTTCG
ACTTTCGCGCGAACGCGGCCGGACAAATCAAGTTGCTCGAGGTGAATCCG
AATCCGGCATGGTGCTGGGACGGCAAGCTCGCGCACATGGGCGAACTCGC
GCACCTGAGCCACAGCGATGTGCTCGCGCGGATTATTGCCGCGGCCGAGC
GGCGCATTGCCGCCGACGGCGAACGCACATCAGACGTGCGCTGA
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