mRNA_M-pyrifera_M_contig87642.20355.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig87642.20355.1
Unique NamemRNA_M-pyrifera_M_contig87642.20355.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig87642.20355.1 vs. uniprot
Match: D8LNW5_ECTSI (Proline--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LNW5_ECTSI)

HSP 1 Score: 94.7 bits (234), Expect = 3.980e-21
Identity = 50/67 (74.63%), Postives = 57/67 (85.07%), Query Frame = 1
Query:    1 LRFAVPDILDSVLCVAQGAVSPLAVVNDKECLATLAMDKGLIDADEVLVHPLRNDRSVRVKASDLVK 201
            LRFA    LDSVL V QGAVSPLAVVND E +A LAMDK +++A+EVLVHPLRNDRSVR+KA+DLVK
Sbjct:  270 LRFADAATLDSVLGVVQGAVSPLAVVNDVEGVARLAMDKSVMEAEEVLVHPLRNDRSVRIKAADLVK 336          
BLAST of mRNA_M-pyrifera_M_contig87642.20355.1 vs. uniprot
Match: A0A5D6Y8W5_9STRA (Proline--tRNA ligase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6Y8W5_9STRA)

HSP 1 Score: 64.7 bits (156), Expect = 1.450e-10
Identity = 33/67 (49.25%), Postives = 47/67 (70.15%), Query Frame = 1
Query:    1 LRFAVPDILDSVLCVAQGAVSPLAVVNDKECLATLAMDKGLIDADEVLVHPLRNDRSVRVKASDLVK 201
            LRFA  D+L+SVL V +GAVSPL V++D +    L +DK L+DA  V  HPLRNDR+  + ++ L++
Sbjct:   88 LRFAGEDVLESVLKVKKGAVSPLCVMHDTDNAVRLVLDKALVDAAVVHCHPLRNDRTYALASTALLQ 154          
BLAST of mRNA_M-pyrifera_M_contig87642.20355.1 vs. uniprot
Match: A0A7S3ANH3_9EUKA (Proline--tRNA ligase n=1 Tax=Haptolina ericina TaxID=156174 RepID=A0A7S3ANH3_9EUKA)

HSP 1 Score: 64.7 bits (156), Expect = 1.450e-10
Identity = 33/66 (50.00%), Postives = 44/66 (66.67%), Query Frame = 1
Query:    4 RFAVPDILDSVLCVAQGAVSPLAVVNDKECLATLAMDKGLIDADEVLVHPLRNDRSVRVKASDLVK 201
            RFA  ++L  +L V QG+VSPLAV+ND     TL +DK L+ A+ V VHPLRND + ++   DL K
Sbjct:   90 RFATAEVLQELLGVQQGSVSPLAVMNDTTGAVTLCIDKELMQANYVGVHPLRNDMTNQISPDDLTK 155          
BLAST of mRNA_M-pyrifera_M_contig87642.20355.1 vs. uniprot
Match: A0A7S3H5B0_9STRA (Proline--tRNA ligase n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3H5B0_9STRA)

HSP 1 Score: 63.5 bits (153), Expect = 3.700e-10
Identity = 30/66 (45.45%), Postives = 42/66 (63.64%), Query Frame = 1
Query:    1 LRFAVPDILDSVLCVAQGAVSPLAVVNDKECLATLAMDKGLIDADEVLVHPLRNDRSVRVKASDLV 198
            +RF   D+L   L V +GAVSP A+VND        +DK L+DA+ + +HPLRNDR+  +  +DLV
Sbjct:   84 VRFGDEDLLKEKLAVIRGAVSPFALVNDSAAEVKFVIDKALLDAELINIHPLRNDRTTSIPPADLV 149          
BLAST of mRNA_M-pyrifera_M_contig87642.20355.1 vs. uniprot
Match: A0A7X4ERS9_9PROT (Prolyl-tRNA synthetase associated domain-containing protein n=3 Tax=Rhodospirillaceae bacterium TaxID=1898112 RepID=A0A7X4ERS9_9PROT)

HSP 1 Score: 60.1 bits (144), Expect = 1.720e-9
Identity = 29/66 (43.94%), Postives = 47/66 (71.21%), Query Frame = 1
Query:    1 LRFAVPDILDSVLCVAQGAVSPLAVVNDKECLATLAMDKGLIDADEVLVHPLRNDRSVRVKASDLV 198
            + FA  D+++ VL V  GA++PLAV+ND   L T+A++ GL+DAD++  HPL N  S+ ++ ++LV
Sbjct:   92 MGFAPADLVERVLGVMPGALTPLAVLNDDRGLVTVAIEAGLMDADQLNFHPLVNTESIGLEPAELV 157          
BLAST of mRNA_M-pyrifera_M_contig87642.20355.1 vs. uniprot
Match: A0A3D5I4S0_9PROT (Prolyl-tRNA synthetase associated domain-containing protein n=1 Tax=Alphaproteobacteria bacterium TaxID=1913988 RepID=A0A3D5I4S0_9PROT)

HSP 1 Score: 59.7 bits (143), Expect = 2.600e-9
Identity = 28/65 (43.08%), Postives = 43/65 (66.15%), Query Frame = 1
Query:    7 FAVPDILDSVLCVAQGAVSPLAVVNDKECLATLAMDKGLIDADEVLVHPLRNDRSVRVKASDLVK 201
            F   D L   L V  GAVSPL ++ND +   TL +D+ ++DAD+V +HPL ND++V +  +DL++
Sbjct:  103 FGSADRLMEFLGVRPGAVSPLTLINDPDHQVTLVIDRAILDADKVNLHPLVNDKTVTISTTDLIR 167          
BLAST of mRNA_M-pyrifera_M_contig87642.20355.1 vs. uniprot
Match: K3WC49_GLOUD (Proline--tRNA ligase n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WC49_GLOUD)

HSP 1 Score: 60.8 bits (146), Expect = 3.300e-9
Identity = 30/67 (44.78%), Postives = 45/67 (67.16%), Query Frame = 1
Query:    1 LRFAVPDILDSVLCVAQGAVSPLAVVNDKECLATLAMDKGLIDADEVLVHPLRNDRSVRVKASDLVK 201
            LRFA  D+L++VL V +G+VSPL V++D++    L +DK L+DA  +  HPLRND +  +    L+K
Sbjct:   86 LRFATEDVLETVLKVKKGSVSPLCVMHDEKTEVRLVLDKALVDAKVLHCHPLRNDLTYSLAPEALLK 152          
BLAST of mRNA_M-pyrifera_M_contig87642.20355.1 vs. uniprot
Match: A0A7S0SYX4_9STRA (Hypothetical protein (Fragment) n=1 Tax=Chromulina nebulosa TaxID=96789 RepID=A0A7S0SYX4_9STRA)

HSP 1 Score: 58.5 bits (140), Expect = 5.280e-9
Identity = 29/65 (44.62%), Postives = 41/65 (63.08%), Query Frame = 1
Query:    1 LRFAVPDILDSVLCVAQGAVSPLAVVNDKECLATLAMDKGLIDADEVLVHPLRNDRSVRVKASDL 195
            LRF   D+L   L V +GAVSPLA++NDK       +D  L+  D + +HPLRND++  +K +DL
Sbjct:   83 LRFGEEDLLLEKLGVIRGAVSPLALINDKNNEVKFCIDSELLLGDNINIHPLRNDKTTSLKPNDL 147          
BLAST of mRNA_M-pyrifera_M_contig87642.20355.1 vs. uniprot
Match: A0A7Y3W525_9PROT (Prolyl-tRNA synthetase associated domain-containing protein n=1 Tax=Parvularcula mediterranea TaxID=2732508 RepID=A0A7Y3W525_9PROT)

HSP 1 Score: 56.2 bits (134), Expect = 4.000e-8
Identity = 27/66 (40.91%), Postives = 41/66 (62.12%), Query Frame = 1
Query:    1 LRFAVPDILDSVLCVAQGAVSPLAVVNDKEC-LATLAMDKGLIDADEVLVHPLRNDRSVRVKASDL 195
            L FA P++++ VL VA G ++P A++ND+   + T  +D+ L +AD +  HPLRN  S  V   DL
Sbjct:   82 LSFAKPEVMEEVLGVAPGHLTPFALINDRSLRIETFVIDEALFEADPIWAHPLRNTASTGVTGEDL 147          
BLAST of mRNA_M-pyrifera_M_contig87642.20355.1 vs. uniprot
Match: A0A418VRZ3_9PROT (Prolyl-tRNA synthetase associated domain-containing protein n=1 Tax=Azospirillum cavernae TaxID=2320860 RepID=A0A418VRZ3_9PROT)

HSP 1 Score: 55.8 bits (133), Expect = 6.700e-8
Identity = 30/65 (46.15%), Postives = 38/65 (58.46%), Query Frame = 1
Query:    1 LRFAVPDILDSVLCVAQGAVSPLAVVNDKECLATLAMDKGLIDADEVLVHPLRNDRSVRVKASDL 195
            L FA  D+L + L V QGAVSP A+VND+E    L +D  L+ A  V  HPL N  +  V+  DL
Sbjct:   89 LSFAKEDLLFATLGVRQGAVSPFALVNDRERRVRLVLDAALMAAPRVAFHPLTNTATTAVRTDDL 153          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig87642.20355.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LNW5_ECTSI3.980e-2174.63Proline--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879... [more]
A0A5D6Y8W5_9STRA1.450e-1049.25Proline--tRNA ligase n=1 Tax=Pythium brassicum Tax... [more]
A0A7S3ANH3_9EUKA1.450e-1050.00Proline--tRNA ligase n=1 Tax=Haptolina ericina Tax... [more]
A0A7S3H5B0_9STRA3.700e-1045.45Proline--tRNA ligase n=1 Tax=Spumella elongata Tax... [more]
A0A7X4ERS9_9PROT1.720e-943.94Prolyl-tRNA synthetase associated domain-containin... [more]
A0A3D5I4S0_9PROT2.600e-943.08Prolyl-tRNA synthetase associated domain-containin... [more]
K3WC49_GLOUD3.300e-944.78Proline--tRNA ligase n=1 Tax=Globisporangium ultim... [more]
A0A7S0SYX4_9STRA5.280e-944.62Hypothetical protein (Fragment) n=1 Tax=Chromulina... [more]
A0A7Y3W525_9PROT4.000e-840.91Prolyl-tRNA synthetase associated domain-containin... [more]
A0A418VRZ3_9PROT6.700e-846.15Prolyl-tRNA synthetase associated domain-containin... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig87642contigM-pyrifera_M_contig87642:385..585 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score94.7
Seed ortholog evalue1.7e-17
Seed eggNOG ortholog2880.D8LNW5
KEGG rclassRC00004,RC00049,RC00055,RC00069,RC00296,RC00523,RC00840,RC00948,RC01704,RC01705,RC01706,RC01758,RC01759,RC01765,RC01767,RC01769,RC02243,RC02527,RC02939,RC02940,RC02942,RC02943,RC02944
KEGG koko:K00799,ko:K01120,ko:K01194,ko:K01874,ko:K01881,ko:K15437,ko:K22119
KEGG TC1.A.12.2.2,1.A.12.3.2
KEGG ReactionR00010,R00191,R01234,R03522,R03659,R03661,R04773,R07002,R07003,R07004,R07023,R07024,R07025,R07026,R07069,R07070,R07083,R07084,R07091,R07092,R07093,R07094,R07100,R07113,R07116,R08280,R09409,R11905
KEGG Pathwayko00230,ko00450,ko00480,ko00500,ko00970,ko00980,ko00982,ko00983,ko01100,ko01524,ko05200,ko05204,ko05225,ko05418,map00230,map00450,map00480,map00500,map00970,map00980,map00982,map00983,map01100,map01524,map05200,map05204,map05225,map05418
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryother localisation
EggNOG free text desc.synthetase
EggNOG OGsCOG0442@1,COG0625@1,KOG0867@2759,KOG4163@2759
EC2.5.1.18,3.1.4.17,3.2.1.28,6.1.1.10,6.1.1.15
COG Functional cat.J
CAZyGH37
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko00537,ko01000,ko01007,ko02000,ko03016
Exons1
Model size201
Cds size201
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816785.338572-CDS-M-pyrifera_M_contig87642:384..5851622816785.338572-CDS-M-pyrifera_M_contig87642:384..585Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87642 385..585 -
1692278177.2294395-CDS-M-pyrifera_M_contig87642:384..5851692278177.2294395-CDS-M-pyrifera_M_contig87642:384..585Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87642 385..585 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig87642.20355.1prot_M-pyrifera_M_contig87642.20355.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig87642 385..585 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig87642.20355.1

>prot_M-pyrifera_M_contig87642.20355.1 ID=prot_M-pyrifera_M_contig87642.20355.1|Name=mRNA_M-pyrifera_M_contig87642.20355.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=67bp
LRFAVPDILDSVLCVAQGAVSPLAVVNDKECLATLAMDKGLIDADEVLVH
PLRNDRSVRVKASDLVK
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mRNA from alignment at M-pyrifera_M_contig87642:385..585-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig87642.20355.1 ID=mRNA_M-pyrifera_M_contig87642.20355.1|Name=mRNA_M-pyrifera_M_contig87642.20355.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=201bp|location=Sequence derived from alignment at M-pyrifera_M_contig87642:385..585- (Macrocystis pyrifera P11B4 male)
CTCCGCTTCGCAGTCCCCGACATTCTGGACTCCGTCCTCTGCGTGGCCCA GGGTGCGGTTAGCCCTCTGGCGGTCGTTAACGATAAGGAGTGCTTGGCGA CGCTGGCTATGGACAAGGGGCTCATCGACGCCGACGAGGTGCTCGTACAC CCGCTGAGGAACGACCGGAGCGTGCGCGTCAAGGCCTCCGATCTTGTCAA G
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig87642:385..585-

>mRNA_M-pyrifera_M_contig87642.20355.1 ID=mRNA_M-pyrifera_M_contig87642.20355.1|Name=mRNA_M-pyrifera_M_contig87642.20355.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=402bp|location=Sequence derived from alignment at M-pyrifera_M_contig87642:385..585- (Macrocystis pyrifera P11B4 male)
CTCCGCTTCGCAGTCCCCGACATTCTGGACTCCGTCCTCTGCGTGGCCCA
GGGTGCGGTTAGCCCTCTGGCGGTCGTTAACGATAAGGAGTGCTTGGCGA
CGCTGGCTATGGACAAGGGGCTCATCGACGCCGACGAGGTGCTCGTACAC
CCGCTGAGGAACGACCGGAGCGTGCGCGTCAAGGCCTCCGATCTTGTCAA
GCTCCGCTTCGCAGTCCCCGACATTCTGGACTCCGTCCTCTGCGTGGCCC
AGGGTGCGGTTAGCCCTCTGGCGGTCGTTAACGATAAGGAGTGCTTGGCG
ACGCTGGCTATGGACAAGGGGCTCATCGACGCCGACGAGGTGCTCGTACA
CCCGCTGAGGAACGACCGGAGCGTGCGCGTCAAGGCCTCCGATCTTGTCA
AG
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