mRNA_M-pyrifera_M_contig87419.20315.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig87419.20315.1
Unique NamemRNA_M-pyrifera_M_contig87419.20315.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig87419.20315.1 vs. uniprot
Match: G4HJA8_9BACL (LamG domain protein jellyroll fold domain protein n=7 Tax=Paenibacillus TaxID=44249 RepID=G4HJA8_9BACL)

HSP 1 Score: 75.1 bits (183), Expect = 3.940e-12
Identity = 63/190 (33.16%), Postives = 94/190 (49.47%), Query Frame = 1
Query:   49 ANSGSNTVSSVDVFVAG-QTYPCYRQP-VLVAANATLLAFSEARNNT-NCSGTVDGYPKWIVLRRSMDGGKSWGAIQTLYEGSIDFL---TALYDPRMGRSHLMIQ----------------QGSTTVEYTTSDDDGATWSS----IKAIPMSGSQYSVTKPAVGHGIVLGHGGAPCATGKPGRLLMPFV 540
            A  G     ++D+FVAG + Y  +R P VL   + TLLAF+E R N+ + +G +D     +VL+RSMDGG +WG++QT+ +   D     TA+ D   GR  L +                 +G  T+    SDDDGA+WS      + +     ++  T PA+G  I L HG         GRL++P +
Sbjct:   38 AADGEAQFRNIDLFVAGTEGYHTFRIPTVLTTMDGTLLAFAEGRKNSASDTGDID-----LVLKRSMDGGTTWGSLQTVCDMGTDTCGNPTAVQDESNGRIWLFMNHNYGEDTIEEINNGTSRGVRTIWSAYSDDDGASWSEPVNRFEEVQPPDIRWDATGPAIG--IQLRHGP------NQGRLVIPAI 214          
BLAST of mRNA_M-pyrifera_M_contig87419.20315.1 vs. uniprot
Match: UPI001EF166BB (exo-alpha-sialidase n=1 Tax=Brachybacterium sp. ACRRE TaxID=2918184 RepID=UPI001EF166BB)

HSP 1 Score: 74.3 bits (181), Expect = 6.730e-12
Identity = 69/195 (35.38%), Postives = 93/195 (47.69%), Query Frame = 1
Query:  106 YPCYRQPVLVAA-NATLLAFSEARNNTNC--SGTVDGYPKWIVLRRSMDGGKSWGAIQTLYEGSIDFLTA---LYDPRMGRSHLMIQQGSTT--------VEYTTSDDDGATWSSIKAIP-----MSGSQYSVTKPAVGHGIVLGHGGAPCATGKPGRLLMPFVCAGKSNAQTSSTADIACPGCFSCVAMSDDGG 633
            Y C+R P +V   + TLLAF+E R ++ C  SG +D      V+RRS DGGK+WGA++ + +  +D +     + D   GR  L+      T        V+Y  SDDDG TWS  K I       S  ++  T P  GHGI L HG  P A    GR+L+     G  +   S     A       +A SDDGG
Sbjct:   75 YFCFRIPAVVETKDGTLLAFAEGRKDSGCGDSGNID-----TVVRRSHDGGKTWGAVELIDDAGVDTIGGPLPIVDQETGRIVLITTHNPATNHNLRTPYVQY--SDDDGKTWSKRKNIKDDIMDPSWQKWFATGP--GHGIQLKHG--PHA----GRMLIGLNHEGYISGSESHDTGAA-------LAYSDDGG 247          
BLAST of mRNA_M-pyrifera_M_contig87419.20315.1 vs. uniprot
Match: A0A7V2IK98_9BACT (Exo-alpha-sialidase n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7V2IK98_9BACT)

HSP 1 Score: 73.2 bits (178), Expect = 1.550e-11
Identity = 63/161 (39.13%), Postives = 85/161 (52.80%), Query Frame = 1
Query:   82 DVFVAG-QTYPCYRQP-VLVAANATLLAFSEARNNTNCS-GTVDGYPKWIVLRRSMDGGKSWGAIQTLYE-GSIDFLTA-----LYDPRMGRSHLMIQQGSTTVEYTTSDDDGATWSSIKAIPMSGSQYSVTKPAVGHGIVLGHGGAPCATGK-PGRLLMP 534
            DVFV+G + Y  YR P +LVAAN  LLAF E R  ++   G VD     IVL+RS+DGG++WG ++ LYE G    +T      + D R GR  L   + +  V    SDD G TWS  + I  + +    T  A G G+     G   A G+  GRL++P
Sbjct:   53 DVFVSGTEGYHTYRIPALLVAANGDLLAFCEGRKTSSADHGDVD-----IVLKRSLDGGRTWGPLELLYEEGDTATITIGNPCPVLDRRTGRIWLPFCRDNRRVFVGYSDDHGKTWSPRREITDTVADPDWTWYATGPGV-----GIQLAHGEHAGRLVIP 203          
BLAST of mRNA_M-pyrifera_M_contig87419.20315.1 vs. uniprot
Match: A0A1R1SSP8_9ACTN (Neuraminidase n=7 Tax=Streptomyces TaxID=1883 RepID=A0A1R1SSP8_9ACTN)

HSP 1 Score: 71.6 bits (174), Expect = 5.620e-11
Identity = 72/199 (36.18%), Postives = 94/199 (47.24%), Query Frame = 1
Query:  106 YPCYRQPVLVAA-NATLLAFSEAR-NNTNCSGTVDGYPKWIVLRRSMDGGKSWGAIQTLYEGSIDFL---TALYDPRMGRSHLMI--------QQG-----STTVEYTTSDDDGATWS-----SIKAIPMSGSQYSVTKPAVGHGIVLGHGGAPCATGKPGRLLMPFVCAGKSNAQTSSTADIACPGCFSCVAMSDDGG 633
            Y C+R P +V + + TLLAF+E R NN   +G +D     +VL+RS DGG++WG +Q + EG+ D       + D R GR  L           QG       T     SDDDG TWS     S   +P S + +  T P   HGI L HG         GRLL  F    +S+  T  TA+ A       +A SDDGG
Sbjct:   45 YSCFRIPAVVKSKDGTLLAFAEGRVNNCGDAGDID-----LVLKRSTDGGRTWGPLQVINEGARDTHGNPAPVVDLRTGRILLASTYNTGRDDSQGCDVPCDRTPHLQYSDDDGVTWSTPRDLSDSLLPASWNSWYATGPV--HGIQLTHGRYR------GRLL--FTINTESHDGTRITANHAA------LAYSDDGG 222          
BLAST of mRNA_M-pyrifera_M_contig87419.20315.1 vs. uniprot
Match: A0A3Q9G2V7_STRLT (Laminin G n=3 Tax=Streptomyces TaxID=1883 RepID=A0A3Q9G2V7_STRLT)

HSP 1 Score: 69.7 bits (169), Expect = 2.600e-10
Identity = 65/198 (32.83%), Postives = 87/198 (43.94%), Query Frame = 1
Query:  106 YPCYRQPVLVAA-NATLLAFSEARNNTNCSGTVDGYPKWIVLRRSMDGGKSWGAIQTLYEGSIDFL---TALYDPRMGRSHLMIQQGS-------------TTVEYTTSDDDGATWSSIKAI-----PMSGSQYSVTKPAVGHGIVLGHGGAPCATGKPGRLLMPFVCAGKSNAQTSSTADIACPGCFSCVAMSDDGG 633
            Y CYR P +V   + TLLAF+E R + NC    D     +VL+RS DGG+SWGA+Q +  G  D       + D R GR  L                    T     SDDDG +WS  + I     P   + +  T P  GHG+ LGHG         GRL++          +TS+          + +A+SDDGG
Sbjct:   90 YACYRIPAIVTTTHGTLLAFAEGRKD-NCGDATD---IDLVLKRSTDGGRSWGALQIVDPGRGDTHGNPAPVVDRRTGRIVLATTYNEGREDAGNCDVPCDRTPHLQYSDDDGVSWSWPRDITEEIRPPGWNSWYATGP--GHGVQLGHGP------HEGRLVIGLNAESHDGTRTSAN--------HAALALSDDGG 267          
BLAST of mRNA_M-pyrifera_M_contig87419.20315.1 vs. uniprot
Match: A0A7H8IJX1_9ACTN (Exo-alpha-sialidase n=2 Tax=Streptomyces TaxID=1883 RepID=A0A7H8IJX1_9ACTN)

HSP 1 Score: 69.3 bits (168), Expect = 3.480e-10
Identity = 70/201 (34.83%), Postives = 93/201 (46.27%), Query Frame = 1
Query:  100 QTYPCYRQPVLVAA-NATLLAFSEAR-NNTNCSGTVDGYPKWIVLRRSMDGGKSWGAIQTLYEGSIDFL---TALYDPRMGRSHLMIQQGS-------------TTVEYTTSDDDGATWS-----SIKAIPMSGSQYSVTKPAVGHGIVLGHGGAPCATGKPGRLLMPFVCAGKSNAQTSSTADIACPGCFSCVAMSDDGG 633
            Q Y C+R P +V + + TLLAF+E R +N   +G +D     +VL+RS DGG++WG +Q + EG  D       + D R GR  L     +              T     SDDDGATWS     S   +P S + +  T P   HGI L HG         GRLL  F    +S+  T  T + A       +A SDDGG
Sbjct:   64 QGYSCFRIPAVVKSKDGTLLAFAEGRVDNCGDAGDID-----LVLKRSTDGGRTWGPLQVINEGGGDTHGNPAPIVDLRTGRIVLASTYNTGRDDSQSCDVPCDRTPHLQYSDDDGATWSAPTDLSPALLPPSWNSWYATGPV--HGIQLRHGR------HKGRLL--FTVNTESHDGTRITHNHAA------LAYSDDGG 243          
BLAST of mRNA_M-pyrifera_M_contig87419.20315.1 vs. uniprot
Match: UPI001E4224D0 (glycoside hydrolase n=1 Tax=Streptomyces sp. UNOB3_S3 TaxID=2871682 RepID=UPI001E4224D0)

HSP 1 Score: 68.6 bits (166), Expect = 5.340e-10
Identity = 64/198 (32.32%), Postives = 88/198 (44.44%), Query Frame = 1
Query:  106 YPCYRQPVLVA-ANATLLAFSEARNNTNCSGTVDGYPKWIVLRRSMDGGKSWGAIQTLYEGSIDFL---TALYDPRMGRSHLMIQQGS-------------TTVEYTTSDDDGATWSSIKAI-----PMSGSQYSVTKPAVGHGIVLGHGGAPCATGKPGRLLMPFVCAGKSNAQTSSTADIACPGCFSCVAMSDDGG 633
            Y CYR P +V  A+ TLLAF+E R + NC    D     +VL+RS DGG++WG +Q +  G  D       + D R GR  L                    T     SDDDGA+WS  + +     P   + +  T P  GHG+ LGHG         GRL++          +TS+          + +A+SDDGG
Sbjct:   34 YACYRIPAIVTTAHGTLLAFAEGRKD-NCGDATD---IDLVLKRSTDGGRTWGPLQIVDPGLGDTHGNPAPVVDRRTGRIVLATTYNEGREDAGNCDVPCDRTPHLQYSDDDGASWSWPRDLTEEIRPPGWNSWYATGP--GHGVQLGHGP------HEGRLVIGLNAESHDGTRTSAN--------HAALALSDDGG 211          
BLAST of mRNA_M-pyrifera_M_contig87419.20315.1 vs. uniprot
Match: A0A6I4MIB4_9ACTN (Neuraminidase n=2 Tax=Actinomadura physcomitrii TaxID=2650748 RepID=A0A6I4MIB4_9ACTN)

HSP 1 Score: 68.2 bits (165), Expect = 8.390e-10
Identity = 62/160 (38.75%), Postives = 84/160 (52.50%), Query Frame = 1
Query:  106 YPCYRQPVLVAA-NATLLAFSEARNNT-NCSGTVDGYPKWIVLRRSMDGGKSWGAIQTLYEGSIDFL---TALYDPRMGRSHLMIQQG---STTVEYTT---SDDDGATWSSIKAI------PMSGSQYSVTKPAVGHGIVLGHGGAPCATGKPGRLLMP 534
            Y C+R P +V A + TLLAF+E R N+   +G +D     +VLRRS DGG++WG +Q +  G+ D       + D R GR  LM         T+ +     SDDDGATWS+ + +      P   S Y+ T P  GH I L HG  P A    GRL++P
Sbjct:   32 YFCFRIPAVVKALDGTLLAFAEGRRNSCGDAGDID-----LVLRRSADGGRTWGPLQVVLPGNGDTRGNPAPVVDRRTGRIVLMTTWNPGDDDTIRHPFVQYSDDDGATWSAARDLTGQLVKPEWNSWYA-TGP--GHAIQLRHG--PHA----GRLIVP 177          
BLAST of mRNA_M-pyrifera_M_contig87419.20315.1 vs. uniprot
Match: UPI000E2398B5 (exo-alpha-sialidase n=1 Tax=Streptomyces olivoreticuli TaxID=68246 RepID=UPI000E2398B5)

HSP 1 Score: 66.6 bits (161), Expect = 2.910e-9
Identity = 70/212 (33.02%), Postives = 91/212 (42.92%), Query Frame = 1
Query:   85 VFVAGQ-------TYPCYRQPVLVA-ANATLLAFSEARNNTNCSGTVDGYPKWIVLRRSMDGGKSWGAIQTLYEGSIDFL---TALYDPRMGRSHLMIQQGS-------------TTVEYTTSDDDGATWSSIKAI-----PMSGSQYSVTKPAVGHGIVLGHGGAPCATGKPGRLLMPFVCAGKSNAQTSSTADIACPGCFSCVAMSDDGG 633
            +F AGQ        Y C+R P LV  A   LLAF+E R + NC    D     +VL+RS DGG++WG ++ + EG  D       + D R GR  L     +              T     SDDDG TWS    I     P   + +  T P  GHGI L HG        P R  +      +S+  T  T+D A       +A+SDDGG
Sbjct:   65 LFKAGQGEGDTERDYACFRIPALVTTARGALLAFAEGRRH-NCGDATD---IDLVLKRSTDGGRTWGPLRVVDEGGGDTHGNPAPVVDRRTGRIVLATTHNAGRPDAGNCDVPCDRTPHVQYSDDDGTTWSRPHDITREIRPRGWNSWYATGP--GHGIQLAHG--------PHRGRLVVGVNAESHNGTRVTSDHAA------LALSDDGG 256          
BLAST of mRNA_M-pyrifera_M_contig87419.20315.1 vs. uniprot
Match: UPI001AEFB9B3 (exo-alpha-sialidase n=2 Tax=Ruania zhangjianzhongii TaxID=2603206 RepID=UPI001AEFB9B3)

HSP 1 Score: 66.6 bits (161), Expect = 3.000e-9
Identity = 54/153 (35.29%), Postives = 75/153 (49.02%), Query Frame = 1
Query:   85 VFVAGQT-YPCYRQPVLVAAN-ATLLAFSEARNNTNC--SGTVDGYPKWIVLRRSMDGGKSWGAIQTLYEGSIDFL---TALYDPRMGRSHLM------IQQGSTTVEYTTSDDDGATWSSIKAI-----PMSGSQYSVTKPAVGHGIVLGHG 489
            +F  G+  Y C+R P +V  N  TLLAF+E R ++ C  SG +D      VLRRS DGG +WG I+ +++  +D     T + D   GR  L+      +     T     S+DDGATWS  + I          ++  T P  G GI L HG
Sbjct:   59 IFERGEDGYFCFRIPAVVETNDGTLLAFAEGRRSSGCDDSGDID-----TVLRRSQDGGDTWGPIEVVFDAGVDTAGNPTPVVDSDSGRIALITTHNPALNHNLRTPYLQYSEDDGATWSEPEDIGDDITDPDWEKWLATGP--GAGIQLDHG 204          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig87419.20315.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
G4HJA8_9BACL3.940e-1233.16LamG domain protein jellyroll fold domain protein ... [more]
UPI001EF166BB6.730e-1235.38exo-alpha-sialidase n=1 Tax=Brachybacterium sp. AC... [more]
A0A7V2IK98_9BACT1.550e-1139.13Exo-alpha-sialidase n=1 Tax=Phycisphaerales bacter... [more]
A0A1R1SSP8_9ACTN5.620e-1136.18Neuraminidase n=7 Tax=Streptomyces TaxID=1883 RepI... [more]
A0A3Q9G2V7_STRLT2.600e-1032.83Laminin G n=3 Tax=Streptomyces TaxID=1883 RepID=A0... [more]
A0A7H8IJX1_9ACTN3.480e-1034.83Exo-alpha-sialidase n=2 Tax=Streptomyces TaxID=188... [more]
UPI001E4224D05.340e-1032.32glycoside hydrolase n=1 Tax=Streptomyces sp. UNOB3... [more]
A0A6I4MIB4_9ACTN8.390e-1038.75Neuraminidase n=2 Tax=Actinomadura physcomitrii Ta... [more]
UPI000E2398B52.910e-933.02exo-alpha-sialidase n=1 Tax=Streptomyces olivoreti... [more]
UPI001AEFB9B33.000e-935.29exo-alpha-sialidase n=2 Tax=Ruania zhangjianzhongi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig87419contigM-pyrifera_M_contig87419:125..760 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score76.3
Seed ortholog evalue2e-11
Seed eggNOG ortholog743719.PaelaDRAFT_4069
KEGG rclassRC00028,RC00077
KEGG koko:K01186
KEGG ReactionR04018
KEGG Pathwayko00511,ko00600,ko04142,map00511,map00600,map04142
Hectar predicted targeting categorysignal peptide
EggNOG free text desc.An automated process has identified a potential problem with this gene model
EggNOG OGs1TQRX@1239,4HEUW@91061,COG0265@1,COG0265@2,COG2010@1,COG2010@2,COG4409@1,COG4409@2
EC3.2.1.18
COG Functional cat.G
CAZyGH33
Best tax levelBacilli
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko02042
Exons1
Model size636
Cds size636
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816783.1486728-CDS-M-pyrifera_M_contig87419:124..7601622816783.1486728-CDS-M-pyrifera_M_contig87419:124..760Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87419 125..760 +
1692278175.7836747-CDS-M-pyrifera_M_contig87419:124..7601692278175.7836747-CDS-M-pyrifera_M_contig87419:124..760Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87419 125..760 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig87419.20315.1prot_M-pyrifera_M_contig87419.20315.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig87419 125..760 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig87419.20315.1

>prot_M-pyrifera_M_contig87419.20315.1 ID=prot_M-pyrifera_M_contig87419.20315.1|Name=mRNA_M-pyrifera_M_contig87419.20315.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=212bp
MLVSSLLVACLLHFVHANSGSNTVSSVDVFVAGQTYPCYRQPVLVAANAT
LLAFSEARNNTNCSGTVDGYPKWIVLRRSMDGGKSWGAIQTLYEGSIDFL
TALYDPRMGRSHLMIQQGSTTVEYTTSDDDGATWSSIKAIPMSGSQYSVT
KPAVGHGIVLGHGGAPCATGKPGRLLMPFVCAGKSNAQTSSTADIACPGC
FSCVAMSDDGG*
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mRNA from alignment at M-pyrifera_M_contig87419:125..760+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig87419.20315.1 ID=mRNA_M-pyrifera_M_contig87419.20315.1|Name=mRNA_M-pyrifera_M_contig87419.20315.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=636bp|location=Sequence derived from alignment at M-pyrifera_M_contig87419:125..760+ (Macrocystis pyrifera P11B4 male)
ATGCTTGTTTCGAGTCTACTGGTTGCTTGTTTGCTTCATTTTGTGCACGC AAACTCGGGTTCAAACACTGTTTCAAGTGTGGATGTATTTGTGGCTGGTC AAACCTATCCGTGTTACCGACAACCAGTGCTAGTGGCGGCCAATGCGACG CTTTTAGCGTTTTCTGAAGCGAGAAACAACACCAACTGTTCTGGAACAGT CGATGGATACCCAAAATGGATCGTTCTTCGCCGATCTATGGATGGAGGCA AGAGTTGGGGTGCAATTCAGACCTTGTACGAAGGATCCATCGACTTCCTC ACCGCCCTGTACGACCCACGGATGGGAAGATCCCATCTGATGATTCAACA AGGTTCCACTACTGTGGAGTATACTACATCAGATGATGATGGTGCAACCT GGAGTAGCATCAAGGCGATTCCTATGTCTGGATCGCAATATTCTGTAACA AAGCCTGCTGTCGGACATGGTATTGTGTTAGGTCATGGAGGAGCTCCATG TGCTACAGGAAAACCTGGTAGATTACTAATGCCATTTGTTTGTGCTGGCA AGAGCAATGCACAAACCTCTTCCACCGCTGATATTGCATGCCCTGGTTGT TTCTCGTGTGTTGCCATGTCTGATGATGGTGGATAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig87419:125..760+

>mRNA_M-pyrifera_M_contig87419.20315.1 ID=mRNA_M-pyrifera_M_contig87419.20315.1|Name=mRNA_M-pyrifera_M_contig87419.20315.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1272bp|location=Sequence derived from alignment at M-pyrifera_M_contig87419:125..760+ (Macrocystis pyrifera P11B4 male)
ATGCTTGTTTCGAGTCTACTGGTTGCTTGTTTGCTTCATTTTGTGCACGC
AAACTCGGGTTCAAACACTGTTTCAAGTGTGGATGTATTTGTGGCTGGTC
AAACCTATCCGTGTTACCGACAACCAGTGCTAGTGGCGGCCAATGCGACG
CTTTTAGCGTTTTCTGAAGCGAGAAACAACACCAACTGTTCTGGAACAGT
CGATGGATACCCAAAATGGATCGTTCTTCGCCGATCTATGGATGGAGGCA
AGAGTTGGGGTGCAATTCAGACCTTGTACGAAGGATCCATCGACTTCCTC
ACCGCCCTGTACGACCCACGGATGGGAAGATCCCATCTGATGATTCAACA
AGGTTCCACTACTGTGGAGTATACTACATCAGATGATGATGGTGCAACCT
GGAGTAGCATCAAGGCGATTCCTATGTCTGGATCGCAATATTCTGTAACA
AAGCCTGCTGTCGGACATGGTATTGTGTTAGGTCATGGAGGAGCTCCATG
TGCTACAGGAAAACCTGGTAGATTACTAATGCCATTTGTTTGTGCTGGCA
AGAGCAATGCACAAACCTCTTCCACCGCTGATATTGCATGCCCTGGTTGT
TTCTCGTGTGTTGCCATGTCTGATGATGGTGGATAGATGCTTGTTTCGAG
TCTACTGGTTGCTTGTTTGCTTCATTTTGTGCACGCAAACTCGGGTTCAA
ACACTGTTTCAAGTGTGGATGTATTTGTGGCTGGTCAAACCTATCCGTGT
TACCGACAACCAGTGCTAGTGGCGGCCAATGCGACGCTTTTAGCGTTTTC
TGAAGCGAGAAACAACACCAACTGTTCTGGAACAGTCGATGGATACCCAA
AATGGATCGTTCTTCGCCGATCTATGGATGGAGGCAAGAGTTGGGGTGCA
ATTCAGACCTTGTACGAAGGATCCATCGACTTCCTCACCGCCCTGTACGA
CCCACGGATGGGAAGATCCCATCTGATGATTCAACAAGGTTCCACTACTG
TGGAGTATACTACATCAGATGATGATGGTGCAACCTGGAGTAGCATCAAG
GCGATTCCTATGTCTGGATCGCAATATTCTGTAACAAAGCCTGCTGTCGG
ACATGGTATTGTGTTAGGTCATGGAGGAGCTCCATGTGCTACAGGAAAAC
CTGGTAGATTACTAATGCCATTTGTTTGTGCTGGCAAGAGCAATGCACAA
ACCTCTTCCACCGCTGATATTGCATGCCCTGGTTGTTTCTCGTGTGTTGC
CATGTCTGATGATGGTGGATAG
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