mRNA_M-pyrifera_M_contig87219.20278.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig87219.20278.1
Unique NamemRNA_M-pyrifera_M_contig87219.20278.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig87219.20278.1 vs. uniprot
Match: UPI00064522A8 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI00064522A8)

HSP 1 Score: 77.0 bits (188), Expect = 2.860e-13
Identity = 32/79 (40.51%), Postives = 58/79 (73.42%), Query Frame = 1
Query:    4 EVVRKSLPMLLHPNTWVRYAVVSVVTQVAAQLDDCDVFCFIIPHVRPYLIGDI-DAVTVELILEGLQAPIARQTFERAV 237
            E++ KS P+LLHPNTW+RY V+S++T++A +L   D +C++ P V  +L+ D+ D ++ + +L+ L +PI+R++F + V
Sbjct:  782 ELLAKSTPLLLHPNTWIRYGVISLITKIAGKLSKTDFYCYVKPRVSQFLVADVSDIISEDNLLQLLISPISRESFNKMV 860          
BLAST of mRNA_M-pyrifera_M_contig87219.20278.1 vs. uniprot
Match: D3AWP9_POLPP (Non-specific serine/threonine protein kinase n=1 Tax=Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) TaxID=670386 RepID=D3AWP9_POLPP)

HSP 1 Score: 70.5 bits (171), Expect = 5.240e-11
Identity = 29/79 (36.71%), Postives = 56/79 (70.89%), Query Frame = 1
Query:    4 EVVRKSLPMLLHPNTWVRYAVVSVVTQVAAQLDDCDVFCFIIPHVRPYLIGDI-DAVTVELILEGLQAPIARQTFERAV 237
            +++ ++ P+LLHPNTW+RY V+S++T+++++L   D +C++ P + P+L+ DI D +T   +L+ L   I+R  F++ V
Sbjct:  450 QLLARASPLLLHPNTWIRYGVISLITKISSRLSKTDFYCYVKPRISPFLVADINDQLTEANLLQLLIPAISRDAFKKMV 528          
BLAST of mRNA_M-pyrifera_M_contig87219.20278.1 vs. uniprot
Match: F0ZP95_DICPU (Non-specific serine/threonine protein kinase n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0ZP95_DICPU)

HSP 1 Score: 70.5 bits (171), Expect = 5.380e-11
Identity = 29/79 (36.71%), Postives = 54/79 (68.35%), Query Frame = 1
Query:    4 EVVRKSLPMLLHPNTWVRYAVVSVVTQVAAQLDDCDVFCFIIPHVRPYLIGD---IDAVTVELILEGLQAPIARQTFER 231
            E++ K+ PMLLHPN W+RY VVS++ ++ + L   D++C++ P + P+L+G+    D ++   +++ L  PI+R++F R
Sbjct:  764 ELLVKTSPMLLHPNNWIRYGVVSLIVKICSSLSKADLYCYVKPKLAPFLVGNGEISDNISESNLIQLLDTPISRESFNR 842          
BLAST of mRNA_M-pyrifera_M_contig87219.20278.1 vs. uniprot
Match: A0A5A9N2F2_9TELE (Non-specific serine/threonine protein kinase n=1 Tax=Triplophysa tibetana TaxID=1572043 RepID=A0A5A9N2F2_9TELE)

HSP 1 Score: 70.1 bits (170), Expect = 7.150e-11
Identity = 42/143 (29.37%), Postives = 75/143 (52.45%), Query Frame = 1
Query:    1 FEVVRKSLPMLLHPNTWVRYAVVSVVTQVAAQLDDCDVFCFIIPHVRPYLIGDIDAVTVELILEG-LQAPIARQTFERAVAVCSESLQKFYRSAGARARVGDKKTHVDPVAPYDPDDPFLEVVLEDFANSGLAGEKETLLLML 426
            +E V    P   HPN W+RY  V  +T VA  L+  DV+C ++PH++P++   I  + ++++L   L+ P++R  F+ A+   S+ +   +R    R +   K+    P  P  P+DP +  +L+   + G+   +E  LL L
Sbjct:  656 YEFVSDIAPFSCHPNLWIRYGAVGFITVVAQHLNIADVYCKLMPHLKPFITQPIIQIDIKIVLLSVLKEPVSRSIFDYALR--SKDIGSLFRHLLLRQK---KRNGSIPECPI-PEDPAIAQLLKKLLSQGMTEAEEDKLLAL 792          
BLAST of mRNA_M-pyrifera_M_contig87219.20278.1 vs. uniprot
Match: F4PUG5_CAVFA (Non-specific serine/threonine protein kinase n=1 Tax=Cavenderia fasciculata (strain SH3) TaxID=1054147 RepID=F4PUG5_CAVFA)

HSP 1 Score: 68.9 bits (167), Expect = 1.830e-10
Identity = 28/76 (36.84%), Postives = 52/76 (68.42%), Query Frame = 1
Query:    4 EVVRKSLPMLLHPNTWVRYAVVSVVTQVAAQLDDCDVFCFIIPHVRPYLIGDI--DAVTVELILEGLQAPIARQTF 225
            E++ +S P+LLHPN W+RY  +S+++++A +L   D++C++ P ++PYLI D   D +    + + L  PI+R++F
Sbjct:  748 EILSRSSPLLLHPNDWIRYGTISLISKIATKLSKTDIYCYLKPRIQPYLITDSSSDNIAESNLFQLLIPPISRESF 823          
BLAST of mRNA_M-pyrifera_M_contig87219.20278.1 vs. uniprot
Match: A0A8J4V3F4_9MYCE (Non-specific serine/threonine protein kinase n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4V3F4_9MYCE)

HSP 1 Score: 68.9 bits (167), Expect = 1.830e-10
Identity = 32/82 (39.02%), Postives = 58/82 (70.73%), Query Frame = 1
Query:    4 EVVRKSLPMLLHPNTWVRYAVVSVVTQVAAQLDDCDVFCFIIPHVRPYLI--GDI--DAVTVELILEGLQAPIARQTFERAV 237
            E++ K+ PMLLHPNTW+RY V+S++T++ A L   DV+C+  P +  +L+  G+I  D ++   +L+ +++PI+R++F + V
Sbjct:  817 ELLVKTSPMLLHPNTWIRYGVISLITKICACLSKADVYCYAKPRITKFLVESGEIISDNLSEANLLQLIESPISRESFYKVV 898          
BLAST of mRNA_M-pyrifera_M_contig87219.20278.1 vs. uniprot
Match: A0A1Y1YME5_9FUNG (Non-specific serine/threonine protein kinase n=1 Tax=Basidiobolus meristosporus CBS 931.73 TaxID=1314790 RepID=A0A1Y1YME5_9FUNG)

HSP 1 Score: 68.2 bits (165), Expect = 3.340e-10
Identity = 32/84 (38.10%), Postives = 51/84 (60.71%), Query Frame = 1
Query:    4 EVVRKSLPMLLHPNTWVRYAVVSVVTQVAAQLDDCDVFCFIIPHVRPYLIGDIDAVTVELILEGLQAPIARQTFERAVAVCSES 255
            E+V   +P++ HPN WVRY V+ V+   +  +   DV+CFI P ++PYL  DI   T E ++E ++  IAR+ F+  +   +ES
Sbjct:  688 EIVSALMPLIYHPNVWVRYGVIRVIVSGSNAMAITDVWCFIYPIIQPYLKADILDFTEEELIESVRPAIAREVFDEVLLWANES 771          
BLAST of mRNA_M-pyrifera_M_contig87219.20278.1 vs. uniprot
Match: A0A2P6NLL2_9EUKA (Non-specific serine/threonine protein kinase n=1 Tax=Planoprotostelium fungivorum TaxID=1890364 RepID=A0A2P6NLL2_9EUKA)

HSP 1 Score: 67.8 bits (164), Expect = 4.750e-10
Identity = 30/69 (43.48%), Postives = 45/69 (65.22%), Query Frame = 1
Query:    4 EVVRKSLPMLLHPNTWVRYAVVSVVTQVAAQLDDCDVFCFIIPHVRPYLIGDIDAVTVELILEGLQAPI 210
            ++V K+ P+L+HPNTW+RY  V++++ VA QL   DV CF+IP + P+L   I  V    IL+ L  P+
Sbjct:  732 DIVEKTAPLLVHPNTWIRYGTVAIMSAVATQLKSVDVHCFLIPALTPFLHTPIQNVDQTNILQALVTPV 800          
BLAST of mRNA_M-pyrifera_M_contig87219.20278.1 vs. uniprot
Match: H3AQ45_LATCH (Non-specific serine/threonine protein kinase n=3 Tax=Latimeria chalumnae TaxID=7897 RepID=H3AQ45_LATCH)

HSP 1 Score: 67.0 bits (162), Expect = 8.360e-10
Identity = 43/143 (30.07%), Postives = 72/143 (50.35%), Query Frame = 1
Query:    1 FEVVRKSLPMLLHPNTWVRYAVVSVVTQVAAQLDDCDVFCFIIPHVRPYLIGDIDAVTVELILEG-LQAPIARQTFERAVAVCSESLQKFYRSAGARARVGDKKTHVDPVAPYDPDDPFLEVVLEDFANSGLAGEKETLLLML 426
            +E      P L HPN W+RY  V  +T VA  L+  DV+C ++P ++P++   I  +  EL+L   L+ P++R  F+  +   S+ +   +R    R R   K+    P  P  P+DP +  +L+   + G+   +E  LL L
Sbjct:  653 YEFSSDIAPFLCHPNLWIRYGAVGFITVVAQHLNIADVYCKLMPFLKPFITHPIIQIDKELVLLSVLKEPVSRSIFDYTLR--SKDIGSLFRHLQLRQR---KRNGTIPDCPP-PEDPAIAQLLKKLLSQGMTEAEEDKLLAL 789          
BLAST of mRNA_M-pyrifera_M_contig87219.20278.1 vs. uniprot
Match: VPS15_DICDI (Probable serine/threonine-protein kinase vps15 n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=VPS15_DICDI)

HSP 1 Score: 66.6 bits (161), Expect = 1.170e-9
Identity = 31/88 (35.23%), Postives = 55/88 (62.50%), Query Frame = 1
Query:    4 EVVRKSLPMLLHPNTWVRYAVVSVVTQVAAQLDDCDVFCFIIPHVRPYLIG---------DI-DAVTVELILEGLQAPIARQTFERAV 237
            E++ K+ PMLLHPN W+RY VVS++ ++   L   DV+C++ P + P+L+          +I D +T   +++ L +PI+R++F + V
Sbjct:  800 ELLVKTSPMLLHPNNWIRYGVVSLIVKICQSLSKADVYCYVKPKLSPFLVNLNGXXXXXAEISDTITESNLIQLLDSPISRESFNKIV 887          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig87219.20278.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI00064522A82.860e-1340.51hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
D3AWP9_POLPP5.240e-1136.71Non-specific serine/threonine protein kinase n=1 T... [more]
F0ZP95_DICPU5.380e-1136.71Non-specific serine/threonine protein kinase n=1 T... [more]
A0A5A9N2F2_9TELE7.150e-1129.37Non-specific serine/threonine protein kinase n=1 T... [more]
F4PUG5_CAVFA1.830e-1036.84Non-specific serine/threonine protein kinase n=1 T... [more]
A0A8J4V3F4_9MYCE1.830e-1039.02Non-specific serine/threonine protein kinase n=1 T... [more]
A0A1Y1YME5_9FUNG3.340e-1038.10Non-specific serine/threonine protein kinase n=1 T... [more]
A0A2P6NLL2_9EUKA4.750e-1043.48Non-specific serine/threonine protein kinase n=1 T... [more]
H3AQ45_LATCH8.360e-1030.07Non-specific serine/threonine protein kinase n=3 T... [more]
VPS15_DICDI1.170e-935.23Probable serine/threonine-protein kinase vps15 n=1... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig87219contigM-pyrifera_M_contig87219:3..512 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score71.2
Seed ortholog evalue4.8e-10
Seed eggNOG ortholog8083.ENSXMAP00000000846
Preferred namePIK3R4
KEGG koko:K08333
KEGG Pathwayko04136,ko04138,ko04140,ko04371,map04136,map04138,map04140,map04371
Hectar predicted targeting categoryother localisation
GOsGO:0001894,GO:0002165,GO:0002218,GO:0002221,GO:0002224,GO:0002253,GO:0002376,GO:0002682,GO:0002684,GO:0002697,GO:0002700,GO:0002757,GO:0002758,GO:0002759,GO:0002764,GO:0002784,GO:0002831,GO:0002920,GO:0003674,GO:0003824,GO:0004672,GO:0004674,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005635,GO:0005643,GO:0005737,GO:0005768,GO:0005770,GO:0005829,GO:0005856,GO:0005929,GO:0005930,GO:0005942,GO:0006464,GO:0006468,GO:0006605,GO:0006623,GO:0006629,GO:0006644,GO:0006650,GO:0006661,GO:0006793,GO:0006796,GO:0006807,GO:0006810,GO:0006886,GO:0006914,GO:0006950,GO:0007034,GO:0007154,GO:0007163,GO:0007165,GO:0007275,GO:0007399,GO:0007552,GO:0008104,GO:0008150,GO:0008152,GO:0008219,GO:0008610,GO:0008654,GO:0009056,GO:0009057,GO:0009058,GO:0009267,GO:0009605,GO:0009653,GO:0009791,GO:0009886,GO:0009893,GO:0009987,GO:0009991,GO:0010562,GO:0010564,GO:0010623,GO:0010669,GO:0012501,GO:0012505,GO:0012506,GO:0015031,GO:0015630,GO:0015833,GO:0016020,GO:0016192,GO:0016236,GO:0016301,GO:0016303,GO:0016310,GO:0016322,GO:0016740,GO:0016772,GO:0016773,GO:0019216,GO:0019220,GO:0019222,GO:0019538,GO:0019637,GO:0019898,GO:0021700,GO:0022008,GO:0023052,GO:0030139,GO:0030154,GO:0030182,GO:0030242,GO:0030258,GO:0030659,GO:0030666,GO:0030670,GO:0031090,GO:0031323,GO:0031325,GO:0031347,GO:0031349,GO:0031410,GO:0031667,GO:0031668,GO:0031669,GO:0031967,GO:0031975,GO:0031982,GO:0032101,GO:0032465,GO:0032501,GO:0032502,GO:0032801,GO:0032838,GO:0032991,GO:0033036,GO:0033365,GO:0033554,GO:0033674,GO:0034162,GO:0034198,GO:0034271,GO:0034272,GO:0034613,GO:0035004,GO:0035032,GO:0035069,GO:0035096,GO:0036092,GO:0036211,GO:0042149,GO:0042325,GO:0042327,GO:0042551,GO:0042592,GO:0042594,GO:0042886,GO:0042995,GO:0043085,GO:0043112,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043412,GO:0043549,GO:0043550,GO:0043551,GO:0043552,GO:0043900,GO:0044093,GO:0044232,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044255,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044430,GO:0044433,GO:0044441,GO:0044444,GO:0044446,GO:0044463,GO:0044464,GO:0045017,GO:0045088,GO:0045089,GO:0045184,GO:0045324,GO:0045335,GO:0045834,GO:0045937,GO:0046474,GO:0046486,GO:0046488,GO:0046834,GO:0046854,GO:0046907,GO:0048468,GO:0048469,GO:0048518,GO:0048522,GO:0048583,GO:0048584,GO:0048666,GO:0048699,GO:0048707,GO:0048731,GO:0048856,GO:0048869,GO:0048871,GO:0050776,GO:0050778,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051174,GO:0051179,GO:0051234,GO:0051302,GO:0051338,GO:0051347,GO:0051641,GO:0051649,GO:0051716,GO:0051726,GO:0052742,GO:0060033,GO:0060249,GO:0061695,GO:0061919,GO:0065007,GO:0065008,GO:0065009,GO:0070727,GO:0071496,GO:0071561,GO:0071695,GO:0071702,GO:0071704,GO:0071705,GO:0072594,GO:0072665,GO:0072666,GO:0080090,GO:0080134,GO:0090218,GO:0090407,GO:0097014,GO:0097708,GO:0098588,GO:0098796,GO:0098805,GO:0099568,GO:0120025,GO:0120038,GO:0140096,GO:1901564,GO:1901575,GO:1901576,GO:1902494,GO:1903725,GO:1903727,GO:1990234,GO:1990928
EggNOG free text desc.regulatory subunit 4
EggNOG OGs38C2I@33154,3BC53@33208,3CWDJ@33213,47ZF3@7711,48VY5@7742,49YC2@7898,KOG1240@1,KOG1240@2759
EC2.7.11.1
COG Functional cat.T
Best tax levelActinopterygii
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01001,ko04131
Exons1
Model size510
Cds size483
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816781.7120833-UTR-M-pyrifera_M_contig87219:2..291622816781.7120833-UTR-M-pyrifera_M_contig87219:2..29Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig87219 3..29 +
1692278175.012862-UTR-M-pyrifera_M_contig87219:2..291692278175.012862-UTR-M-pyrifera_M_contig87219:2..29Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig87219 3..29 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816781.7249227-CDS-M-pyrifera_M_contig87219:29..5121622816781.7249227-CDS-M-pyrifera_M_contig87219:29..512Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87219 30..512 +
1692278175.034624-CDS-M-pyrifera_M_contig87219:29..5121692278175.034624-CDS-M-pyrifera_M_contig87219:29..512Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87219 30..512 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig87219.20278.1prot_M-pyrifera_M_contig87219.20278.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig87219 30..512 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig87219.20278.1

>prot_M-pyrifera_M_contig87219.20278.1 ID=prot_M-pyrifera_M_contig87219.20278.1|Name=mRNA_M-pyrifera_M_contig87219.20278.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=161bp
MLLHPNTWVRYAVVSVVTQVAAQLDDCDVFCFIIPHVRPYLIGDIDAVTV
ELILEGLQAPIARQTFERAVAVCSESLQKFYRSAGARARVGDKKTHVDPV
APYDPDDPFLEVVLEDFANSGLAGEKETLLLMLPYIRESTRGSHRMESEG
RSRAAANGTAD
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mRNA from alignment at M-pyrifera_M_contig87219:3..512+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig87219.20278.1 ID=mRNA_M-pyrifera_M_contig87219.20278.1|Name=mRNA_M-pyrifera_M_contig87219.20278.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=510bp|location=Sequence derived from alignment at M-pyrifera_M_contig87219:3..512+ (Macrocystis pyrifera P11B4 male)
TTTGAGGTCGTGCGAAAGTCTCTCCCCATGCTCCTCCATCCCAACACATG GGTGCGCTATGCGGTGGTCTCTGTTGTCACCCAGGTGGCGGCCCAGTTGG ACGACTGTGACGTCTTCTGCTTCATCATTCCTCACGTGAGACCATATTTG ATTGGAGACATTGATGCTGTGACTGTGGAGCTTATTTTGGAGGGACTCCA AGCTCCCATAGCTCGTCAGACCTTCGAGAGAGCGGTGGCAGTCTGCAGTG AGAGTCTGCAGAAGTTCTACCGCTCTGCGGGAGCGAGGGCCAGAGTGGGA GACAAGAAAACGCACGTGGACCCAGTGGCACCCTACGATCCTGACGACCC CTTCTTGGAGGTGGTCCTTGAGGACTTTGCCAACTCTGGCCTGGCAGGCG AGAAAGAGACGCTCCTCTTGATGTTGCCTTACATTCGAGAGTCCACGCGT GGCAGCCACCGCATGGAGAGCGAAGGGAGGAGCAGAGCGGCGGCAAACGG CACCGCAGAT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig87219:3..512+

>mRNA_M-pyrifera_M_contig87219.20278.1 ID=mRNA_M-pyrifera_M_contig87219.20278.1|Name=mRNA_M-pyrifera_M_contig87219.20278.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=966bp|location=Sequence derived from alignment at M-pyrifera_M_contig87219:3..512+ (Macrocystis pyrifera P11B4 male)
ATGCTCCTCCATCCCAACACATGGGTGCGCTATGCGGTGGTCTCTGTTGT
CACCCAGGTGGCGGCCCAGTTGGACGACTGTGACGTCTTCTGCTTCATCA
TTCCTCACGTGAGACCATATTTGATTGGAGACATTGATGCTGTGACTGTG
GAGCTTATTTTGGAGGGACTCCAAGCTCCCATAGCTCGTCAGACCTTCGA
GAGAGCGGTGGCAGTCTGCAGTGAGAGTCTGCAGAAGTTCTACCGCTCTG
CGGGAGCGAGGGCCAGAGTGGGAGACAAGAAAACGCACGTGGACCCAGTG
GCACCCTACGATCCTGACGACCCCTTCTTGGAGGTGGTCCTTGAGGACTT
TGCCAACTCTGGCCTGGCAGGCGAGAAAGAGACGCTCCTCTTGATGTTGC
CTTACATTCGAGAGTCCACGCGTGGCAGCCACCGCATGGAGAGCGAAGGG
AGGAGCAGAGCGGCGGCAAACGGCACCGCAGATATGCTCCTCCATCCCAA
CACATGGGTGCGCTATGCGGTGGTCTCTGTTGTCACCCAGGTGGCGGCCC
AGTTGGACGACTGTGACGTCTTCTGCTTCATCATTCCTCACGTGAGACCA
TATTTGATTGGAGACATTGATGCTGTGACTGTGGAGCTTATTTTGGAGGG
ACTCCAAGCTCCCATAGCTCGTCAGACCTTCGAGAGAGCGGTGGCAGTCT
GCAGTGAGAGTCTGCAGAAGTTCTACCGCTCTGCGGGAGCGAGGGCCAGA
GTGGGAGACAAGAAAACGCACGTGGACCCAGTGGCACCCTACGATCCTGA
CGACCCCTTCTTGGAGGTGGTCCTTGAGGACTTTGCCAACTCTGGCCTGG
CAGGCGAGAAAGAGACGCTCCTCTTGATGTTGCCTTACATTCGAGAGTCC
ACGCGTGGCAGCCACCGCATGGAGAGCGAAGGGAGGAGCAGAGCGGCGGC
AAACGGCACCGCAGAT
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