mRNA_M-pyrifera_M_contig86695.20165.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig86695.20165.1
Unique NamemRNA_M-pyrifera_M_contig86695.20165.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig86695.20165.1 vs. uniprot
Match: A0A0L0D4G4_THETB (Uncharacterized protein n=1 Tax=Thecamonas trahens ATCC 50062 TaxID=461836 RepID=A0A0L0D4G4_THETB)

HSP 1 Score: 160 bits (404), Expect = 3.350e-42
Identity = 91/167 (54.49%), Postives = 115/167 (68.86%), Query Frame = 1
Query:   19 IHLLQHASETMAGFLVASRHYVTGEDDGVFAFDPRFLVFEFVSDLLLRKRQVELVHQFVASTQSGGSSVQQMIMGAGKTTVICPLLALLLANGKSLVTLVVPAALMNMSRTVMRTCFSNIIAKRVYSFTFDRYSSSVSSNQLMDVRKLRVKMEVSQQRRGVIVTTPE 519
            +HL++H    +A  L A R YV  E   +  FDPRFLVFEF    +LR RQVEL+  F A+   G SSV QMIMGAGKTTVI P+L L+LANG SLVT VVP AL++MSR V+RT FSN++ KR+Y+  FDR  SS SSN +  VR L  K+  ++ +R ++VTTPE
Sbjct: 2765 VHLVEHTGAALARILGAKRAYVD-ETTSLPTFDPRFLVFEFSVGFMLRARQVELIGDFAAAAAQGSSSVHQMIMGAGKTTVIGPMLGLILANGSSLVTQVVPGALLDMSRNVLRTAFSNVMPKRIYTLAFDR--SSPSSNSIDAVRALAAKLRRARAQRALVVTTPE 2928          
BLAST of mRNA_M-pyrifera_M_contig86695.20165.1 vs. uniprot
Match: A0A7S1C4T4_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1C4T4_9STRA)

HSP 1 Score: 151 bits (382), Expect = 3.760e-42
Identity = 88/184 (47.83%), Postives = 125/184 (67.93%), Query Frame = 1
Query:    7 TLATIHLLQHASETMAGFLVASRHYV---TGEDDG----------VFAFDPRFLVFEFVSDLLLRKRQVELVHQFVASTQSGGSSVQQMIMGAGKTTVICPLLALLLANGKSLVTLVVPAALMNMSRTVMRTCFSNIIAKRVYSFTFDRYSSSVSSNQLMDVRKLRVKMEVSQQRRGVIVTTPE 519
            ++A +H+L H ++++AG LV  R Y+     EDD           V+ FDPRFLVFE++S  +LR+RQVE+V QF  S+    S VQQMIMGAGKTTVI PLLAL+LA+G+ LVT+V P+AL+  + +VMR CFS++I KRVY+F FDR S    S+ L  +  L  K++ +++ R ++V+ PE
Sbjct:    4 SVAMVHVLHHTADSVAGHLVIKRRYMHRFRQEDDAGELETAAAETVYEFDPRFLVFEYLSGFVLRRRQVEMVSQFADSSNRRVSMVQQMIMGAGKTTVIAPLLALMLADGERLVTVVTPSALIEQTLSVMRGCFSSLIQKRVYTFQFDRMSKE--SSDLKALTALVQKIDRARRERAIVVSAPE 185          
BLAST of mRNA_M-pyrifera_M_contig86695.20165.1 vs. uniprot
Match: A0A7S0CPY2_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Amorphochlora amoebiformis TaxID=1561963 RepID=A0A7S0CPY2_9EUKA)

HSP 1 Score: 147 bits (372), Expect = 6.630e-38
Identity = 82/169 (48.52%), Postives = 120/169 (71.01%), Query Frame = 1
Query:   13 ATIHLLQHASETMAGFLVASRHYVTGEDDGVFAFDPRFLVFEFVSDLLLRKRQVELVHQFVASTQSGGSSVQQMIMGAGKTTVICPLLALLLANGKSLVTLVVPAALMNMSRTVMRTCFSNIIAKRVYSFTFDRYSSSVSSNQLMDVRKLRVKMEVSQQRRGVIVTTPE 519
            A +H+++H ++ +A  L A R Y T        FDPRFLVFEF++  ++R RQVE++ QF  S +   SSV+QMIMGAGKT+V+ P+LALLLANG+ LVT VVP AL++ +RTVMR  FSN+I+K +Y+F FDR  +S ++N +  + KL  K++ ++ +R ++ TTPE
Sbjct: 2484 AMVHIMEHTAKGVASLLSARRGY-TQLSLTTRGFDPRFLVFEFMAGFMMRPRQVEIILQFAESVRRRQSSVRQMIMGAGKTSVVSPMLALLLANGRVLVTQVVPKALLDQTRTVMRNVFSNVISKPIYTFHFDR--ASEAANSITTLEKLYNKLDRARLQRAIVCTTPE 2649          
BLAST of mRNA_M-pyrifera_M_contig86695.20165.1 vs. uniprot
Match: A0A0L0D9P8_THETB (Uncharacterized protein n=1 Tax=Thecamonas trahens ATCC 50062 TaxID=461836 RepID=A0A0L0D9P8_THETB)

HSP 1 Score: 147 bits (370), Expect = 1.240e-37
Identity = 85/166 (51.20%), Postives = 118/166 (71.08%), Query Frame = 1
Query:   28 LQHASETMAGFLVASRHYVTGED-DGVFAFDPRFLVFEFVSDLLLRKRQVELVHQFVASTQSGGSS-VQQMIMGAGKTTVICPLLALLLANGKSLVTLVVPAALMNMSRTVMRTCFSNIIAKRVYSFTFDRYSSSVSSNQLMDVRKLRVKMEVSQQRRGVIVTTPE 519
            +Q+    +   LV  RHY   E   G F++DPRFLVFE++ D+LLR+RQVE+V+ FVA+++S   + VQQMIMGAGKTTVI PLLAL+LA+GK LV  VVP AL+ MSR VMR+ F+ I+ KRVY+FT+DR       +  + V +L  K++ +++RR V+ TTP+
Sbjct: 2675 VQYEVSNLGELLVCERHYGGVESGSGAFSYDPRFLVFEYIFDILLRQRQVEMVNDFVAASRSESHAMVQQMIMGAGKTTVIGPLLALILADGKQLVMQVVPTALLEMSRNVMRSRFTAIVPKRVYTFTYDRSEP----DSAVSVGELWKKLDAARRRRAVVCTTPD 2836          
BLAST of mRNA_M-pyrifera_M_contig86695.20165.1 vs. uniprot
Match: K8Z7G8_NANGC (Nxn protein (Fragment) n=2 Tax=Nannochloropsis gaditana (strain CCMP526) TaxID=1093141 RepID=K8Z7G8_NANGC)

HSP 1 Score: 135 bits (340), Expect = 4.630e-35
Identity = 86/173 (49.71%), Postives = 119/173 (68.79%), Query Frame = 1
Query:    1 SLTLATIHLLQHASETMAGFLVASRHYVTGEDDGVFAFDPRFLVFEFVSDLLLRKRQVELVHQFVASTQSGGSSVQQMIMGAGKTTVICPLLALLLANGKSLVTLVVPAALMNMSRTVMRTCFSNIIAKRVYSFTFDRYSSSVSSNQLMDVRKLRVKMEVSQQRRGVIVTTPE 519
            S++LA   + Q A++ +A  L A R+Y T    G+   DPRFLVFE V DL+LRKRQVE+V  F AS + G S VQQMIMGAGKTTV+ PLL LLLA+G+ LVT V+P AL+  +R++MR+ F  II KRVY+  F+R   SV  +  + V ++  K++ +++RR V+ T+PE
Sbjct:   35 SVSLAMQRVTQ-ATDNLAELLTAGRYYGTSTAAGL-EVDPRFLVFEAVFDLMLRKRQVEMVTWFQASLEQGVSRVQQMIMGAGKTTVVGPLLTLLLADGQQLVTQVMPTALLEQTRSIMRSRFGQIITKRVYTLEFER---SVDDDVEL-VAEIFGKLDAARRRRSVVCTSPE 201          
BLAST of mRNA_M-pyrifera_M_contig86695.20165.1 vs. uniprot
Match: A0A2R5FYY4_9STRA (Calmodulin n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5FYY4_9STRA)

HSP 1 Score: 139 bits (350), Expect = 6.090e-35
Identity = 86/165 (52.12%), Postives = 109/165 (66.06%), Query Frame = 1
Query:   28 LQHASETMAGFLVASRHYVT--GEDDGVFAFDPRFLVFEFVSDLLLRKRQVELVHQFVASTQSGGSSVQQMIMGAGKTTVICPLLALLLANGKSLVTLVVPAALMNMSRTVMRTCFSNIIAKRVYSFTFDRYSSSVSSNQLMDVRKLRVKMEVSQQRRGVIVTTP 516
            LQ  S+ +   L A RHYV   G   G +  DPRFLVFEF   +LLRK QVELV+ F+ + Q+G S   QMIMGAGKTTV+ PLLALLL +GKSLV  VVP AL+  SR VMR  FS II K VY+F FDR++S       +D R++  K+ ++++ R V+ TTP
Sbjct: 2711 LQLKSDAVVKELAAKRHYVKQPGAGQGRYELDPRFLVFEFTYSILLRKSQVELVYDFMGALQNGDSRCHQMIMGAGKTTVVGPLLALLLGDGKSLVVQVVPNALLEFSRAVMRDKFSAIIQKPVYTFVFDRFTS-------VD-RQMYRKLLIARKSRAVVCTTP 2867          
BLAST of mRNA_M-pyrifera_M_contig86695.20165.1 vs. uniprot
Match: A0A7S3YMY9_9EUKA (Hypothetical protein n=1 Tax=Lotharella globosa TaxID=91324 RepID=A0A7S3YMY9_9EUKA)

HSP 1 Score: 137 bits (344), Expect = 3.770e-34
Identity = 77/165 (46.67%), Postives = 116/165 (70.30%), Query Frame = 1
Query:   34 HASETMAGFLVASRHYV---TGEDDGVFAFDPRFLVFEFVSDLLLRKRQVELVHQFVASTQSGGSSVQQMIMGAGKTTVICPLLALLLANGKSLVTLVVPAALMNMSRTVMRTCFSNIIAKRVYSFTFDRYSSSVSSNQLMDVRKLRVKMEVSQQRRGVIVTTPE 519
            H +  +A  + A RH +   T E +G   FDPR+L+FE++ ++LLRK+QV L++ F+ +     S V+QMIMGAGK+TV+ P+L+L+LA+GKSLVTLVVP++L+ MSR VMR  F+ +I KRV++F+FDR    VS     +V K+  K++ ++  +GV+VTT E
Sbjct:  200 HQATELATLVAAKRHCIGQGTAEKNGPLEFDPRYLIFEYLFNILLRKKQVVLINTFLDANAKRESLVRQMIMGAGKSTVVGPMLSLILADGKSLVTLVVPSSLLEMSRNVMRARFTRVIPKRVHTFSFDRSCVWVS-----EVTKMHSKLKDARATKGVVVTTSE 359          
BLAST of mRNA_M-pyrifera_M_contig86695.20165.1 vs. uniprot
Match: UPI0004F423E1 (NXN protein n=1 Tax=Trypanosoma grayi TaxID=71804 RepID=UPI0004F423E1)

HSP 1 Score: 136 bits (343), Expect = 5.230e-34
Identity = 73/166 (43.98%), Postives = 111/166 (66.87%), Query Frame = 1
Query:   28 LQHASETMAGFLVASRHYVT--GEDDGVFAFDPRFLVFEFVSDLLLRKRQVELVHQFVASTQSGGSSVQQMIMGAGKTTVICPLLALLLANGKSLVTLVVPAALMNMSRTVMRTCFSNIIAKRVYSFTFDRYSSSVSSNQLMDVRKLRVKMEVSQQRRGVIVTTPE 519
            +Q  +      L A RHY T  GED+   A DPR+L+FEF+ ++LLR RQVE+V  FV +T++G S VQQMIMG GKTTV+ PLLAL+LA+G+ LVT V+P AL+  +R ++R CFS ++ K++Y+  FDR     +++    +  L+ K+  + + R ++++ PE
Sbjct: 1191 MQQLANEAIDLLTARRHYATMCGEDNAAVALDPRYLLFEFMHNILLRARQVEMVRWFVDNTRAGVSRVQQMIMGQGKTTVVGPLLALILADGEQLVTQVMPTALLEQTRGILRRCFSVVVPKQIYTLHFDRAFDDTNTSH---IALLQQKLTAAARTRAILISAPE 1353          
BLAST of mRNA_M-pyrifera_M_contig86695.20165.1 vs. uniprot
Match: A0A7S4U905_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4U905_9EUKA)

HSP 1 Score: 130 bits (326), Expect = 5.870e-34
Identity = 75/169 (44.38%), Postives = 113/169 (66.86%), Query Frame = 1
Query:   16 TIHLLQHASETMAGFLVASRHYVTGEDDG-VFAFDPRFLVFEFVSDLLLRKRQVELVHQFVASTQSGGSSVQQMIMGAGKTTVICPLLALLLANGKSLVTLVVPAALMNMSRTVMRTCFSNIIAKRVYSFTFDRYSSSVSSNQLMDVRKLRVKMEVSQQRRGVIVTTPE 519
            +  +++ +S  +A  + A R+YV  + +   F +DPRFLV E+V  +LLRKRQVE+V  FV+S ++G S VQQMIMG GKTTV+ PLL L+LA+G SLVT V+P AL+  +R V+R  F+++I K+VY+  FDR      S +L++   L  K++ +++ R V+   PE
Sbjct:  116 SFQMIEQSSSALAQTVTAKRYYVREQGEKQTFVYDPRFLVVEYVFGILLRKRQVEMVESFVSSIRNGDSRVQQMIMGQGKTTVVGPLLVLILADGNSLVTQVMPTALLEQTRNVLRNRFNSVITKKVYTLQFDR--GWEDSAELVEA--LYAKLDSARRHRCVVCAPPE 280          
BLAST of mRNA_M-pyrifera_M_contig86695.20165.1 vs. uniprot
Match: A0A067CR16_SAPPC (Uncharacterized protein n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067CR16_SAPPC)

HSP 1 Score: 135 bits (340), Expect = 1.340e-33
Identity = 81/163 (49.69%), Postives = 112/163 (68.71%), Query Frame = 1
Query:   34 HASETMAGFLVASRHYVTGEDDGVFAFDPRFLVFEFVSDLLLRKRQVELVHQFVASTQSGGSSVQQMIMGAGKTTVICPLLALLLANGKSLVTLVVPAALMNMSRTVMRTCFSN-IIAKRVYSFTFDRYSSSVSSNQLMDVRKLRVKMEVSQQRRGVIVTTPE 519
            HAS+ +A  L+ASRHY+   D   +A+DPR+L+FEFV +LLLR+RQVE+V  F+ S  +G S VQQMIMGAGKTTV+ PLL  +LA+ K LVT V+P AL+  SR V+R+ FSN I+ KR+++ +FDR   SV  +  M +  L  K++ +     VI  +PE
Sbjct: 2670 HASQDVANGLLASRHYLKERDGQTYAYDPRYLLFEFVFELLLRQRQVEMVESFMTSMHNGVSRVQQMIMGAGKTTVVGPLLTYMLADSKVLVTHVMPTALLEQSRAVLRSRFSNAILQKRIFTLSFDR---SVDDDP-MAILALASKLQRACVHADVICASPE 2828          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig86695.20165.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0L0D4G4_THETB3.350e-4254.49Uncharacterized protein n=1 Tax=Thecamonas trahens... [more]
A0A7S1C4T4_9STRA3.760e-4247.83Hypothetical protein (Fragment) n=1 Tax=Bicosoecid... [more]
A0A7S0CPY2_9EUKA6.630e-3848.52Hypothetical protein (Fragment) n=1 Tax=Amorphochl... [more]
A0A0L0D9P8_THETB1.240e-3751.20Uncharacterized protein n=1 Tax=Thecamonas trahens... [more]
K8Z7G8_NANGC4.630e-3549.71Nxn protein (Fragment) n=2 Tax=Nannochloropsis gad... [more]
A0A2R5FYY4_9STRA6.090e-3552.12Calmodulin n=1 Tax=Hondaea fermentalgiana TaxID=23... [more]
A0A7S3YMY9_9EUKA3.770e-3446.67Hypothetical protein n=1 Tax=Lotharella globosa Ta... [more]
UPI0004F423E15.230e-3443.98NXN protein n=1 Tax=Trypanosoma grayi TaxID=71804 ... [more]
A0A7S4U905_9EUKA5.870e-3444.38Hypothetical protein (Fragment) n=1 Tax=Paramoeba ... [more]
A0A067CR16_SAPPC1.340e-3349.69Uncharacterized protein n=2 Tax=Saprolegnia TaxID=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig86695contigM-pyrifera_M_contig86695:1..519 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score161.0
Seed ortholog evalue5e-37
Seed eggNOG ortholog529818.AMSG_03687T0
Hectar predicted targeting categorysignal peptide
EggNOG free text desc.ryanodine-sensitive calcium-release channel activity
EggNOG OGsKOG2243@1,KOG2243@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons1
Model size519
Cds size519
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816776.1939301-CDS-M-pyrifera_M_contig86695:0..5191622816776.1939301-CDS-M-pyrifera_M_contig86695:0..519Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig86695 1..519 -
1692278171.6565926-CDS-M-pyrifera_M_contig86695:0..5191692278171.6565926-CDS-M-pyrifera_M_contig86695:0..519Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig86695 1..519 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig86695.20165.1prot_M-pyrifera_M_contig86695.20165.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig86695 1..519 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig86695.20165.1

>prot_M-pyrifera_M_contig86695.20165.1 ID=prot_M-pyrifera_M_contig86695.20165.1|Name=mRNA_M-pyrifera_M_contig86695.20165.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=173bp
SLTLATIHLLQHASETMAGFLVASRHYVTGEDDGVFAFDPRFLVFEFVSD
LLLRKRQVELVHQFVASTQSGGSSVQQMIMGAGKTTVICPLLALLLANGK
SLVTLVVPAALMNMSRTVMRTCFSNIIAKRVYSFTFDRYSSSVSSNQLMD
VRKLRVKMEVSQQRRGVIVTTPE
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mRNA from alignment at M-pyrifera_M_contig86695:1..519-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig86695.20165.1 ID=mRNA_M-pyrifera_M_contig86695.20165.1|Name=mRNA_M-pyrifera_M_contig86695.20165.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=519bp|location=Sequence derived from alignment at M-pyrifera_M_contig86695:1..519- (Macrocystis pyrifera P11B4 male)
TCACTTACGCTCGCCACGATTCATCTTCTCCAGCATGCCAGCGAGACGAT GGCTGGCTTCTTGGTTGCCTCCCGTCACTACGTCACTGGCGAAGACGACG GTGTGTTCGCGTTCGATCCCCGTTTCCTCGTCTTTGAATTCGTATCAGAC CTTCTGCTGCGAAAGAGGCAAGTGGAGCTGGTCCATCAGTTCGTTGCGAG TACTCAGTCAGGTGGAAGCTCTGTGCAGCAGATGATCATGGGGGCTGGCA AGACGACTGTCATTTGTCCCCTGCTGGCGCTGCTCCTGGCCAATGGCAAG AGCCTGGTGACCCTCGTGGTACCGGCGGCATTGATGAACATGTCTCGTAC TGTCATGAGAACGTGCTTCTCGAACATCATCGCGAAGAGGGTGTACTCCT TCACCTTCGACCGCTACAGCAGCAGTGTAAGCTCAAATCAGCTCATGGAT GTGAGGAAGCTCCGTGTCAAGATGGAGGTCTCCCAACAGAGGCGAGGCGT TATCGTAACTACCCCTGAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig86695:1..519-

>mRNA_M-pyrifera_M_contig86695.20165.1 ID=mRNA_M-pyrifera_M_contig86695.20165.1|Name=mRNA_M-pyrifera_M_contig86695.20165.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1038bp|location=Sequence derived from alignment at M-pyrifera_M_contig86695:1..519- (Macrocystis pyrifera P11B4 male)
TCACTTACGCTCGCCACGATTCATCTTCTCCAGCATGCCAGCGAGACGAT
GGCTGGCTTCTTGGTTGCCTCCCGTCACTACGTCACTGGCGAAGACGACG
GTGTGTTCGCGTTCGATCCCCGTTTCCTCGTCTTTGAATTCGTATCAGAC
CTTCTGCTGCGAAAGAGGCAAGTGGAGCTGGTCCATCAGTTCGTTGCGAG
TACTCAGTCAGGTGGAAGCTCTGTGCAGCAGATGATCATGGGGGCTGGCA
AGACGACTGTCATTTGTCCCCTGCTGGCGCTGCTCCTGGCCAATGGCAAG
AGCCTGGTGACCCTCGTGGTACCGGCGGCATTGATGAACATGTCTCGTAC
TGTCATGAGAACGTGCTTCTCGAACATCATCGCGAAGAGGGTGTACTCCT
TCACCTTCGACCGCTACAGCAGCAGTGTAAGCTCAAATCAGCTCATGGAT
GTGAGGAAGCTCCGTGTCAAGATGGAGGTCTCCCAACAGAGGCGAGGCGT
TATCGTAACTACCCCTGAGTCACTTACGCTCGCCACGATTCATCTTCTCC
AGCATGCCAGCGAGACGATGGCTGGCTTCTTGGTTGCCTCCCGTCACTAC
GTCACTGGCGAAGACGACGGTGTGTTCGCGTTCGATCCCCGTTTCCTCGT
CTTTGAATTCGTATCAGACCTTCTGCTGCGAAAGAGGCAAGTGGAGCTGG
TCCATCAGTTCGTTGCGAGTACTCAGTCAGGTGGAAGCTCTGTGCAGCAG
ATGATCATGGGGGCTGGCAAGACGACTGTCATTTGTCCCCTGCTGGCGCT
GCTCCTGGCCAATGGCAAGAGCCTGGTGACCCTCGTGGTACCGGCGGCAT
TGATGAACATGTCTCGTACTGTCATGAGAACGTGCTTCTCGAACATCATC
GCGAAGAGGGTGTACTCCTTCACCTTCGACCGCTACAGCAGCAGTGTAAG
CTCAAATCAGCTCATGGATGTGAGGAAGCTCCGTGTCAAGATGGAGGTCT
CCCAACAGAGGCGAGGCGTTATCGTAACTACCCCTGAG
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