mRNA_M-pyrifera_M_contig8616.20077.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig8616.20077.1
Unique NamemRNA_M-pyrifera_M_contig8616.20077.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig8616.20077.1 vs. uniprot
Match: D7FY31_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FY31_ECTSI)

HSP 1 Score: 179 bits (455), Expect = 3.220e-54
Identity = 91/125 (72.80%), Postives = 101/125 (80.80%), Query Frame = 1 
Query:    1 MALMDALQLPESTSGMVMLVMTSLGALLLIYLMYQPA--PAVDLGEPEPDPPEPPRNFTKEQLLEFDGRDDVTPAYIALRDEIFDVSSKPEHYKKNGGYHLFAGHDASYSLATGSLDPTDLDKRW 369
            M  MDALQLP+S+ GM ML  T+LGAL+L+YLM+QPA  PA D  E   D P PPRNFT EQL EFDGRD+ TPAY+ALR E+FDVSSKPEHYK  GGYHLFAGHDASYSLATGSLDP DL+K W
Sbjct:    1 MVQMDALQLPDSSGGMAMLGFTALGALVLMYLMFQPARAPATDDDESAADRPPPPRNFTIEQLHEFDGRDEATPAYVALRGEVFDVSSKPEHYKVGGGYHLFAGHDASYSLATGSLDPADLEKSW 125
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BLAST of mRNA_M-pyrifera_M_contig8616.20077.1 vs. uniprot
Match: A0A7S3Y4U5_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3Y4U5_HETAK)

HSP 1 Score: 91.7 bits (226), Expect = 6.620e-20
Identity = 51/125 (40.80%), Postives = 79/125 (63.20%), Query Frame = 1 
Query:    4 ALMDALQLPESTSGMVMLVMTSLGALLLIYLMYQ-----PAPAVDLGEPEPDPPEPPRNFTKEQLLEFDGRDDVTPAYIALRDEIFDVSSKPEHYKKNGGYHLFAGHDASYSLATGSLDPTDLDK 363
            AL+  L+   S + + +++ T++ + ++  L++        PA    + E +P EPPRNFT EQL ++DG ++  P Y+ALR  +FDVSS P HY K+G Y+LFAGHDA+ +LA  S +  DL+K
Sbjct:    9 ALIHQLKTALSNTSVAVVICTTILSFVITALLFSGRNKAQEPAKKQAQEEEEP-EPPRNFTFEQLKKYDGSEEDEPIYVALRGVVFDVSSAPTHYGKDGAYNLFAGHDATRNLAKMSFEEDDLNK 132
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BLAST of mRNA_M-pyrifera_M_contig8616.20077.1 vs. uniprot
Match: A0A8J2SX87_9STRA (Hypothetical protein n=2 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SX87_9STRA)

HSP 1 Score: 88.2 bits (217), Expect = 6.980e-18
Identity = 52/110 (47.27%), Postives = 64/110 (58.18%), Query Frame = 1 
Query:   64 TSLGALLLIYLMYQPAPAVDLGEPE----------PDPPEPPRNFTKEQLLEFDGRDDVTPAYIALRDEIFDVSSKPEHYKKNGGYHLFAGHDASYSLATGSLDPTDLDK 363
            TS  ALLL  L    A  +   EPE           D  EPPR+FT +QL EFDG+D+ TP Y+A++ ++FDVS     Y   GGY LFAG DAS  LAT SL+  DLD+
Sbjct:   26 TSTVALLLCVLFGAYAAYIITNEPERDGTHAPVNEEDDEEPPRDFTPKQLREFDGKDEDTPLYVAVKGDVFDVSEARSFYGPGGGYALFAGRDASKCLATMSLEEVDLDQ 135
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BLAST of mRNA_M-pyrifera_M_contig8616.20077.1 vs. uniprot
Match: A0A836C9I0_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C9I0_9STRA)

HSP 1 Score: 82.4 bits (202), Expect = 2.110e-16
Identity = 44/78 (56.41%), Postives = 50/78 (64.10%), Query Frame = 1 
Query:  148 PEPPRNFTKEQLLEFDG------RDDVTPAYIALRDEIFDVSSKPEHYKKNGGYHLFAGHDASYSLATGSLDPTDLDK 363
            PEP RNFT EQL  F G       D+  P Y+ALR E+FDVS     Y K G YH+FAGHDAS SLA  SLD +DL+K
Sbjct:   57 PEPLRNFTLEQLKAFTGVNAKGEDDEDVPIYVALRGEVFDVSQARHAYGKEGNYHIFAGHDASVSLARHSLDASDLNK 134
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BLAST of mRNA_M-pyrifera_M_contig8616.20077.1 vs. uniprot
Match: F0Y3I4_AURAN (Cytochrome b5 heme-binding domain-containing protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y3I4_AURAN)

HSP 1 Score: 76.6 bits (187), Expect = 1.000e-15
Identity = 37/66 (56.06%), Postives = 43/66 (65.15%), Query Frame = 1 
Query:  163 NFTKEQLLEFDGRDDVTPAYIALRDEIFDVSSKPEHYKKNGGYHLFAGHDASYSLATGSLDPTDLD 360
            +FT  QL  FDGRDD TP Y+ALR E+FDVS+    Y   GGY +F G DAS  LA  SL+  DLD
Sbjct:    1 DFTPAQLRAFDGRDDDTPVYVALRGEVFDVSAARHFYGPGGGYEMFRGRDASRCLAKMSLEEADLD 66
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BLAST of mRNA_M-pyrifera_M_contig8616.20077.1 vs. uniprot
Match: F0ZPA3_DICPU (Cytochrome b5 heme-binding domain-containing protein n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0ZPA3_DICPU)

HSP 1 Score: 72.8 bits (177), Expect = 8.880e-14
Identity = 35/104 (33.65%), Postives = 64/104 (61.54%), Query Frame = 1 
Query:   76 ALLLIYLMY------QPAPAVDLGEPEPDPPEPPRNFTKEQLLEFDGRDDVTPAYIALRDEIFDVSSKPEHYKKNGGYHLFAGHDASYSLATGSLDPTDLDKRW 369
            A+L+I ++Y       P   V + +P+P P    R++T E+L +++G D+  P +IA++ +I+DV++K   Y   G Y+ F+G+DA+  LA  S +  D++K+W
Sbjct:   11 AVLIIIILYLVKVIASPKEVV-VAKPKPKPQYEKRDYTLEELKQYNGTDETKPIFIAIKGKIYDVTAKSSTYGPGGAYNTFSGNDATICLAKSSFEDADINKKW 113
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BLAST of mRNA_M-pyrifera_M_contig8616.20077.1 vs. uniprot
Match: A0A2S9ZWZ4_RHOTO (Cytochrome b5 heme-binding domain-containing protein n=4 Tax=Rhodosporidium toruloides TaxID=5286 RepID=A0A2S9ZWZ4_RHOTO)

HSP 1 Score: 72.8 bits (177), Expect = 2.540e-13
Identity = 37/87 (42.53%), Postives = 52/87 (59.77%), Query Frame = 1 
Query:  109 APAVDLGEPEPDPPEPPRNFTKEQLLEFDGRDDVTPAYIALRDEIFDVSSKPEHYKKNGGYHLFAGHDASYSLATGSLDPTDLDKRW 369
            APA D  E +P    P   F+ E+L ++DG+D+ TP Y+A++  I+DVS+K + Y    GYH+F G DAS +L   SL P D    W
Sbjct:   88 APAGDQKELDPPKDTP---FSAEELAKYDGKDESTPIYVAIKGRIYDVSAKRDMYGPGCGYHVFVGKDASRALGKSSLKPEDAVADW 171
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BLAST of mRNA_M-pyrifera_M_contig8616.20077.1 vs. uniprot
Match: A0A5C5G0R6_9BASI (Cytochrome b5-like heme/steroid binding domain-containing protein n=1 Tax=Rhodotorula diobovata TaxID=5288 RepID=A0A5C5G0R6_9BASI)

HSP 1 Score: 72.8 bits (177), Expect = 4.960e-13
Identity = 36/82 (43.90%), Postives = 52/82 (63.41%), Query Frame = 1 
Query:  109 APAVDLGEPEPDPPEPPRNFTKEQLLEFDGRDDVTPAYIALRDEIFDVSSKPEHYKKNGGYHLFAGHDASYSLATGSLDPTD 354
            APA D  + +P    P   F+K++L ++DG+DD TP Y+A++ +I+DVS+K + Y    GYH+F G DAS  L   SL P D
Sbjct:  131 APAGDKTQLDPPKDTP---FSKDELAKYDGKDDKTPIYVAIKGQIYDVSAKRDMYGPGCGYHVFVGKDASRGLGKSSLKPED 209
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BLAST of mRNA_M-pyrifera_M_contig8616.20077.1 vs. uniprot
Match: G0SY93_RHOT2 (Cytochrome b5 heme-binding domain-containing protein n=2 Tax=Rhodosporidium toruloides TaxID=5286 RepID=G0SY93_RHOT2)

HSP 1 Score: 72.8 bits (177), Expect = 9.310e-13
Identity = 37/87 (42.53%), Postives = 52/87 (59.77%), Query Frame = 1 
Query:  109 APAVDLGEPEPDPPEPPRNFTKEQLLEFDGRDDVTPAYIALRDEIFDVSSKPEHYKKNGGYHLFAGHDASYSLATGSLDPTDLDKRW 369
            APA D  E +P    P   F+ E+L ++DG+D+ TP Y+A++  I+DVS+K + Y    GYH+F G DAS +L   SL P D    W
Sbjct:  189 APAGDQKELDPPKDTP---FSAEELAKYDGKDESTPIYVAIKGRIYDVSAKRDMYGPGCGYHVFVGKDASRALGKSSLKPEDAVADW 272
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BLAST of mRNA_M-pyrifera_M_contig8616.20077.1 vs. uniprot
Match: A0A3M6VI96_9STRA (Uncharacterized protein n=1 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6VI96_9STRA)

HSP 1 Score: 71.6 bits (174), Expect = 1.640e-12
Identity = 37/67 (55.22%), Postives = 47/67 (70.15%), Query Frame = 1 
Query:  160 RNFTKEQLLEFDGRDDVTPAYIALRDEIFDVSSKPEHYKKNGGYHLFAGHDASYSLATGSLDPTDLD 360
            R F+ E+LL F+G +D  P YIAL DE++DVSSK + Y  + GYHLFAG DAS +LA  S +  DLD
Sbjct:   55 RFFSPEELLPFNG-EDGQPIYIALLDEVYDVSSKRDFYGPDEGYHLFAGRDASRALAKMSFEKQDLD 120
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig8616.20077.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FY31_ECTSI3.220e-5472.80Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A7S3Y4U5_HETAK6.620e-2040.80Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
A0A8J2SX87_9STRA6.980e-1847.27Hypothetical protein n=2 Tax=Pelagomonas calceolat... [more]
A0A836C9I0_9STRA2.110e-1656.41Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
F0Y3I4_AURAN1.000e-1556.06Cytochrome b5 heme-binding domain-containing prote... [more]
F0ZPA3_DICPU8.880e-1433.65Cytochrome b5 heme-binding domain-containing prote... [more]
A0A2S9ZWZ4_RHOTO2.540e-1342.53Cytochrome b5 heme-binding domain-containing prote... [more]
A0A5C5G0R6_9BASI4.960e-1343.90Cytochrome b5-like heme/steroid binding domain-con... [more]
G0SY93_RHOT29.310e-1342.53Cytochrome b5 heme-binding domain-containing prote... [more]
A0A3M6VI96_9STRA1.640e-1255.22Uncharacterized protein n=1 Tax=Peronospora effusa... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig8616contigM-pyrifera_M_contig8616:9394..11762 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score182.6
Seed ortholog evalue1.1e-43
Seed eggNOG ortholog2880.D7FY31
KEGG koko:K17278
Hectar predicted targeting categorysignal anchor
GOsGO:0003674,GO:0005488,GO:0005496,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0006066,GO:0006629,GO:0006694,GO:0006696,GO:0008047,GO:0008150,GO:0008152,GO:0008202,GO:0008204,GO:0008289,GO:0008610,GO:0009058,GO:0009889,GO:0009987,GO:0012505,GO:0016020,GO:0016125,GO:0016126,GO:0016128,GO:0016129,GO:0019216,GO:0019218,GO:0019222,GO:0020037,GO:0030234,GO:0031323,GO:0031326,GO:0031984,GO:0032443,GO:0042175,GO:0043085,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044093,GO:0044107,GO:0044108,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044281,GO:0044283,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0046165,GO:0046890,GO:0046906,GO:0048037,GO:0050789,GO:0050790,GO:0050794,GO:0050810,GO:0062012,GO:0065007,GO:0065009,GO:0071704,GO:0080090,GO:0097159,GO:0097384,GO:0098772,GO:0098827,GO:0106118,GO:1901360,GO:1901362,GO:1901363,GO:1901576,GO:1901615,GO:1901617,GO:1902652,GO:1902653,GO:1902930
EggNOG free text desc.steroid binding
EggNOG OGsKOG1110@1,KOG1110@2759
Ec32 ortholog descriptionCytochrome b5-like heme/steroid binding domain
Ec32 orthologEc-27_006910.1
COG Functional cat.CH
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04147
Exons3
Model size372
Cds size372
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig8616.20077.1prot_M-pyrifera_M_contig8616.20077.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig8616 9394..11762 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816772.0885925-CDS-M-pyrifera_M_contig8616:9393..94231622816772.0885925-CDS-M-pyrifera_M_contig8616:9393..9423Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8616 9394..9423 +
1692278169.3921564-CDS-M-pyrifera_M_contig8616:9393..94231692278169.3921564-CDS-M-pyrifera_M_contig8616:9393..9423Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8616 9394..9423 +
1622816772.099251-CDS-M-pyrifera_M_contig8616:10963..111191622816772.099251-CDS-M-pyrifera_M_contig8616:10963..11119Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8616 10964..11119 +
1692278169.4044178-CDS-M-pyrifera_M_contig8616:10963..111191692278169.4044178-CDS-M-pyrifera_M_contig8616:10963..11119Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8616 10964..11119 +
1622816772.1096268-CDS-M-pyrifera_M_contig8616:11576..117621622816772.1096268-CDS-M-pyrifera_M_contig8616:11576..11762Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8616 11577..11762 +
1692278169.4142478-CDS-M-pyrifera_M_contig8616:11576..117621692278169.4142478-CDS-M-pyrifera_M_contig8616:11576..11762Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8616 11577..11762 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig8616.20077.1

>prot_M-pyrifera_M_contig8616.20077.1 ID=prot_M-pyrifera_M_contig8616.20077.1|Name=mRNA_M-pyrifera_M_contig8616.20077.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=124bp
MALMDALQLPESTSGMVMLVMTSLGALLLIYLMYQPAPAVDLGEPEPDPP
EPPRNFTKEQLLEFDGRDDVTPAYIALRDEIFDVSSKPEHYKKNGGYHLF
AGHDASYSLATGSLDPTDLDKRW*
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mRNA from alignment at M-pyrifera_M_contig8616:9394..11762+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig8616.20077.1 ID=mRNA_M-pyrifera_M_contig8616.20077.1|Name=mRNA_M-pyrifera_M_contig8616.20077.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=2369bp|location=Sequence derived from alignment at M-pyrifera_M_contig8616:9394..11762+ (Macrocystis pyrifera P11B4 male)
ATGGCATTGATGGACGCGCTCCAGCTTCCGGTGCGTGCTTCGAAGGATAT
TTGTTTGGGATCCTATGTGGTGATGGCCTAGGGCTATGCGCGGGATGGGA
ACGTGCCTCTTGTTGATGGCGATTTCTATTGATAATATACTTCCACAGAT
GTGCCACAGGTATATTTGTAAAATAAATTTCGTGTTTCCAGTGTTTGTTT
GAGTGTAATGGTCTCCATTTCCTCCGCCATCATGCTCCTGTTACCCTCGT
TGTGTTTCGATGCTGCATGGTGCATGGTGCCCAAGACCTTGCTAAATACT
AAATATGATGGCTTGTGGTCTGGCTTACACTGCATGCTTACTTTTTCCGT
CCTTGGCCGTGGCGATGCAGCAGTAGCATGCCCCAGAAAAGCCTGGAGTT
ACTACCTGGTACCATGTTTACAGTTGGTGTAATCAGCACTATGGTATGTC
TGTGAACAAGTGAAAAAGCAGCAAGTCCAAAGTGGTTTCCATTTATTATT
GATTGTTGCGACATTCATTATTGTGACATTGCGACAGATGGTATGGTATC
ATCCCAAAGAGGGGCCCAATAGGAACCCAGTACCGGTAGCCAATACGCCA
ATAGGCGCCCGATACACCAATTGTCCAATAGGTGACCGTGGCACCGTGGC
CAATGGGCCCACGAGGGTACTTATGTACCAGGTATAGGTATCTGCATGTG
ACGATGATCTATGCTTTGTAATGTTTTCCGATCTTGGCCCGATGCACATT
AGACCTGTACGAGTACGAGTCGTATATAGCACGATGCAGTTTACCACTAC
GGGGGACCATAGTAAATAGGACCAAATATTGTCATTGAAAATAGGTAAAT
ATATAGGTTTTTGTGTCTACTGTAGCTCCTATTTACCGTGGTCCCCCCCC
CAATACTGTTGTACCAGGTAATTAATGTATAGCAGCGCGCTCTAGGTATC
TGCACACCAGGTATGACGATGAATTATTAATAGTGCTTTGTCATGTGTTT
CGATCTTGGCGCGATGCGCATCAGAGCGGTACGAGTAACGCGCGCAGATT
TACCTGGTACTAATTTAATATTAATAGCTGCAAGTAGCGTCTGTACGAGC
ATCTCGCAGCTGCATGTAGCTTCTGTACGAGCATCTCGCAGCTGCAAATA
GCGTCTGTACGAGGATCTCGCATTTCACAGACAGCAGACAGCAGTGGGTT
CGACCACTGCAAGTATGCCAGGTTCTATTATTAATAATAGGTTCCTTGTT
GGATACCAGGTATGTACTACAACAGTAATACTACTGCTGTAAATCGTGTG
TATGGTAACGATGCCTCTCTCTGGCTCTCGTGATCGCTGTTACCTGGAGC
AAATAATCACCGGTCGTGTCGTATGTAGATGTCGTCATTGGTTCGACGTT
TCGTGCTAACACGTAGGAGCACCATTGCTGCAGTTGTTGGATCATATGTA
TGATGGTATGACGTGTTATGAGGCTGCCGTACCCCTCTCGCGTGCGTGAA
GCTGCTGCTGCGGCGGCGGAGGTCTGATATTTGATGCCAACCGTACTCTG
GCGCTGCCTGGCGTCAACAGGAGTCGACGAGCGGCATGGTGATGCTGGTG
ATGACCTCTCTCGGAGCGCTGCTGCTGATATACCTCATGTACCAACCAGC
GCCCGCAGTCGACCTTGGGGAGCCTGAGCCAGATCCGCCCGAGCCTCCAA
GGAACTTCACCAAGGAACAGCTGCTTGTAAGTATCAAAAACCGGTTTTCA
CCCCGAAGGCGTTCTGCATGCGATGCGATGGTTGAGGTCTTCTGAAGTCT
GATGAACGAGCGGTGCGTAATGAGCGAGCGGCGCGAGCGTTGTTTATTTT
ATTTTTTGGTGTCTCTGAAATGGCGGGTGTCGTGGGTCACCCACCGGCCG
TCAGTATCGGTCTCTCGTAAAGCAGCTTCAGCTCCTTAAGGGAGAAACCC
AGGGAAACTAAATATGCAAGAAATACGCTAAGCGAGAATTTTTACAAGTA
TAAAATGCGTAAACTGAGGTTGGTGTTAGGTGTTGCTTGCCAGGTAGCTA
TGAATACCTGGTCGCTAGCTCTTCTCTAGCTGCTTGTCTAGCTGCTGGTC
GCTAGCTCTTCGCTAATTTTTCGTCAACCGGTCGTTAGCTTGTGTGTTTC
ATCTATGGTACGAACGTTGTTGTGTCTATTCAGGAGTTCGATGGGCGCGA
CGATGTGACACCGGCGTACATCGCGTTGCGAGACGAGATTTTTGACGTGA
GCTCTAAGCCGGAGCACTACAAGAAGAATGGCGGATACCACCTCTTCGCT
GGGCACGACGCGTCGTACAGCCTTGCCACGGGCTCGCTCGACCCGACCGA
TCTCGATAAAAGGTGGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig8616:9394..11762+

>mRNA_M-pyrifera_M_contig8616.20077.1 ID=mRNA_M-pyrifera_M_contig8616.20077.1|Name=mRNA_M-pyrifera_M_contig8616.20077.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=744bp|location=Sequence derived from alignment at M-pyrifera_M_contig8616:9394..11762+ (Macrocystis pyrifera P11B4 male)
ATGGCATTGATGGACGCGCTCCAGCTTCCGATGGCATTGATGGACGCGCT
CCAGCTTCCGGAGTCGACGAGCGGCATGGTGATGCTGGTGATGACCTCTC
TCGGAGCGCTGCTGCTGATATACCTCATGTACCAACCAGCGCCCGCAGTC
GACCTTGGGGAGCCTGAGCCAGATCCGCCCGAGCCTCCAAGGAACTTCAC
CAAGGAACAGCTGCTTGAGTCGACGAGCGGCATGGTGATGCTGGTGATGA
CCTCTCTCGGAGCGCTGCTGCTGATATACCTCATGTACCAACCAGCGCCC
GCAGTCGACCTTGGGGAGCCTGAGCCAGATCCGCCCGAGCCTCCAAGGAA
CTTCACCAAGGAACAGCTGCTTGAGTTCGATGGGCGCGACGATGTGACAC
CGGCGTACATCGCGTTGCGAGACGAGATTTTTGACGTGAGCTCTAAGCCG
GAGCACTACAAGAAGAATGGCGGATACCACCTCTTCGCTGGGCACGACGC
GTCGTACAGCCTTGCCACGGGCTCGCTCGACCCGACCGATCTCGATAAAA
GGTGGTAAGAGTTCGATGGGCGCGACGATGTGACACCGGCGTACATCGCG
TTGCGAGACGAGATTTTTGACGTGAGCTCTAAGCCGGAGCACTACAAGAA
GAATGGCGGATACCACCTCTTCGCTGGGCACGACGCGTCGTACAGCCTTG
CCACGGGCTCGCTCGACCCGACCGATCTCGATAAAAGGTGGTAA
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