mRNA_M-pyrifera_M_contig85790.20003.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig85790.20003.1
Unique NamemRNA_M-pyrifera_M_contig85790.20003.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig85790.20003.1 vs. uniprot
Match: A0A2D8CCS5_9BACT (Uncharacterized protein n=2 Tax=Balneolaceae TaxID=1813606 RepID=A0A2D8CCS5_9BACT)

HSP 1 Score: 204 bits (520), Expect = 6.080e-62
Identity = 101/178 (56.74%), Postives = 131/178 (73.60%), Query Frame = 1
Query:    1 GVKHGLNQWIPGGKLLPVDISLQAGFTQLTLTTPFDVNPE----SGSDIKNDFDPSTWDNQSLDFEASSFTINAIVGKTLPFVSAYVGLGYETSETSIATPGAYPITSVNDDY-NGTTETRTKKIESIVDPIDITIGGENSVRAFGGVQFKLAILKIFANYTAATYSSFNAGFGITFR 519
            GVKHG+NQW+PGGKLLPVDIS+Q G+T+ +    F+VNPE      ++I+N F  +TWD+QS+D E ++ T NAIVGKTLPF+S Y GLGYETS+T++ATPG YPITS N DY N     + K+IE+I  PID+ I G+N +RAF G +F LAI +I A+YT +TYS+FN G G   R
Sbjct:  198 GVKHGINQWLPGGKLLPVDISVQVGYTKFSANANFNVNPEVPQGGNAEIENTFPATTWDDQSIDLETTATTFNAIVGKTLPFISVYGGLGYETSKTTLATPGMYPITSFNPDYANDPMNEKEKRIEAIESPIDLEIEGDNKIRAFAGFRFSLAIFRISASYTQSTYSAFNVGVGFGLR 375          
BLAST of mRNA_M-pyrifera_M_contig85790.20003.1 vs. uniprot
Match: A0A2D9FT35_9BACT (Uncharacterized protein n=1 Tax=Balneola sp. TaxID=2024824 RepID=A0A2D9FT35_9BACT)

HSP 1 Score: 197 bits (500), Expect = 3.910e-59
Identity = 98/176 (55.68%), Postives = 126/176 (71.59%), Query Frame = 1
Query:    1 GVKHGLNQWIPGGKLLPVDISLQAGFTQLTLTTPFDVNPESGSDIKNDFDPSTWDNQSLDFEASSFTINAIVGKTLPFVSAYVGLGYETSETSIATPGAYPITSVNDDYN---GTTETRTKKIESIVDPIDITIGGENSVRAFGGVQFKLAILKIFANYTAATYSSFNAGFGITFR 519
            GV+HG+NQW+PGGK+LPVDI++Q G+T       F+V+PESGSDI N + PS W  Q L+ +  + TINAIVGK LPF+S Y GLG+E+S T+I TPGAYPITS+N +Y+       TR K IESI  PID+ I G N++RAF G +  +AIL+I A+YT +TYSS N G G  FR
Sbjct:  183 GVRHGINQWLPGGKILPVDIAVQFGYTAFNSNAGFEVDPESGSDIYNPYTPSQWQGQGLEMDTKATTINAIVGKKLPFISVYGGLGFESSTTTITTPGAYPITSINPNYDPQSNAENTREKIIESINAPIDLEIKGSNNIRAFVGARLSIAILRISASYTLSTYSSANVGIGFGFR 358          
BLAST of mRNA_M-pyrifera_M_contig85790.20003.1 vs. uniprot
Match: A0A316TWW8_9BACT (Uncharacterized protein n=1 Tax=Rhodohalobacter mucosus TaxID=2079485 RepID=A0A316TWW8_9BACT)

HSP 1 Score: 185 bits (470), Expect = 2.000e-54
Identity = 92/178 (51.69%), Postives = 123/178 (69.10%), Query Frame = 1
Query:    1 GVKHGLNQWIPGGKLLPVDISLQAGFTQLTLTTPFDVNPESGSDIKNDFD--PSTWDNQSLDFEASSFTINAIVGKTLPFVSAYVGLGYETSETSIATPGAYPITSVNDDYN---GTTETRTKKIESIVDPIDITIGGENSVRAFGGVQFKLAILKIFANYTAATYSSFNAGFGITFR 519
            GV+HG+NQW+PGG  LPVDIS+QAG T+L  +   DVNPE G+D+ N F   P  W++QS+  EAS FT N IVGK LP +S + GLG++TS   +++PGAYPITS N D+N   G+ ETR + +E I DPI ++  GENSV AF G + +L  + + A YT + Y + NAG GI+FR
Sbjct:  200 GVRHGINQWLPGGAALPVDISVQAGLTRLNSSFDLDVNPEQGTDVYNPFSATPGVWEDQSIQLEASGFTGNVIVGKNLPVISLFGGLGFQTSTVDLSSPGAYPITSFNPDFNPLDGSEETRQRIVERIDDPITLSYDGENSVHAFAGFRLRLGFIALSATYTQSKYPTLNAGVGISFR 377          
BLAST of mRNA_M-pyrifera_M_contig85790.20003.1 vs. uniprot
Match: A0A1M5GXR4_9BACT (Uncharacterized protein n=1 Tax=Aliifodinibius roseus TaxID=1194090 RepID=A0A1M5GXR4_9BACT)

HSP 1 Score: 184 bits (468), Expect = 3.790e-54
Identity = 90/173 (52.02%), Postives = 123/173 (71.10%), Query Frame = 1
Query:    1 GVKHGLNQWIPGGKLLPVDISLQAGFTQLTLTTPFDVNPESGSDIKNDFDPSTWDNQSLDFEASSFTINAIVGKTLPFVSAYVGLGYETSETSIATPGAYPITSVNDDYNGTTETRTKKIESIVDPIDITIGGENSVRAFGGVQFKLAILKIFANYTAATYSSFNAGFGITFR 519
            G+KH + QW+PGGKL+PV+ISLQAG+T+L  +  FDV+PE GSD++N++DPSTW+ Q     +++FTINA+VGKTLPF+S Y G+GYETS  +I TPG+YP+T  N+D+   +  + KKIE I +PIDI+    NS R   G + KLAI  I A+YT + Y     G G +FR
Sbjct:  204 GLKHDITQWLPGGKLIPVNISLQAGYTKLNSSIAFDVDPEQGSDVENNYDPSTWEGQKAAMRSNAFTINALVGKTLPFISFYGGIGYETSSLTIDTPGSYPVTVPNEDFPDQSN-QPKKIEKIDNPIDISYDDNNSFRGLVGTRIKLAIFNITASYTLSKYPVGQVGVGFSFR 375          
BLAST of mRNA_M-pyrifera_M_contig85790.20003.1 vs. uniprot
Match: UPI00083F69D6 (hypothetical protein n=1 Tax=Rhodohalobacter halophilus TaxID=1812810 RepID=UPI00083F69D6)

HSP 1 Score: 182 bits (462), Expect = 2.990e-53
Identity = 89/178 (50.00%), Postives = 125/178 (70.22%), Query Frame = 1
Query:    1 GVKHGLNQWIPGGKLLPVDISLQAGFTQLTLTTPFDVNPESGSDIKNDF--DPSTWDNQSLDFEASSFTINAIVGKTLPFVSAYVGLGYETSETSIATPGAYPITSVNDDYNGTT---ETRTKKIESIVDPIDITIGGENSVRAFGGVQFKLAILKIFANYTAATYSSFNAGFGITFR 519
            G+KHGLNQW+PGG +LPVD+S+Q G+T+LT    F+V PE GSDI N +  +P+ WD Q+++ EA+ FT N +VGK LP +S Y G+G++TSE ++ +PG+YP+TS N DYN T+   ETR K IE I +P+D++  G NSV A  G + +LAI  I  +YT + Y   N G G++FR
Sbjct:  198 GIKHGLNQWLPGGSVLPVDLSVQLGYTKLTSDYGFEVLPEEGSDIYNPYAGNPALWDGQAIELEATGFTGNLLVGKNLPIISVYGGVGFQTSEMTLISPGSYPVTSFNPDYNPTSSSEETREKIIERIDEPVDLSFSGSNSVHAMAGFRLRLAIFTISGSYTLSNYPVANLGVGLSFR 375          
BLAST of mRNA_M-pyrifera_M_contig85790.20003.1 vs. uniprot
Match: A0A2N0VKV8_9BACT (Uncharacterized protein n=1 Tax=Rhodohalobacter barkolensis TaxID=2053187 RepID=A0A2N0VKV8_9BACT)

HSP 1 Score: 179 bits (455), Expect = 3.330e-52
Identity = 86/178 (48.31%), Postives = 126/178 (70.79%), Query Frame = 1
Query:    1 GVKHGLNQWIPGGKLLPVDISLQAGFTQLTLTTPFDVNPESGSDIKNDF--DPSTWDNQSLDFEASSFTINAIVGKTLPFVSAYVGLGYETSETSIATPGAYPITSVNDDYN---GTTETRTKKIESIVDPIDITIGGENSVRAFGGVQFKLAILKIFANYTAATYSSFNAGFGITFR 519
            GVKHGLNQW+PGG +LPVD+S+Q G+T+LT    F+VNPE G DI N +  +PS W+ QS+D EA+ FT N +VGK LP +S Y G+G+++SE ++ +PG+YP+T  N+DY+   G+ ETR K IE + +PI+++  G NS+ A  G + +L++L I  +YT + Y   N G G++FR
Sbjct:  198 GVKHGLNQWLPGGSVLPVDLSVQLGYTKLTSDFGFEVNPEEGQDIYNPYAGNPSLWEGQSIDMEATGFTGNILVGKNLPIISVYGGIGFQSSEMTLNSPGSYPVTVFNEDYDPLDGSEETREKIIERLDEPINLSFDGSNSIHALAGFRLRLSLLTISGSYTLSNYPVANIGVGLSFR 375          
BLAST of mRNA_M-pyrifera_M_contig85790.20003.1 vs. uniprot
Match: A0A521B6J8_9BACT (Uncharacterized protein n=1 Tax=Aliifodinibius sediminis TaxID=1214077 RepID=A0A521B6J8_9BACT)

HSP 1 Score: 172 bits (436), Expect = 2.280e-49
Identity = 81/173 (46.82%), Postives = 120/173 (69.36%), Query Frame = 1
Query:    1 GVKHGLNQWIPGGKLLPVDISLQAGFTQLTLTTPFDVNPESGSDIKNDFDPSTWDNQSLDFEASSFTINAIVGKTLPFVSAYVGLGYETSETSIATPGAYPITSVNDDYNGTTETRTKKIESIVDPIDITIGGENSVRAFGGVQFKLAILKIFANYTAATYSSFNAGFGITFR 519
            G+KH + +W+PGGKL+PV+ISLQAG+T+L  +  F+V+PE G+D++N++DPSTW+ Q      ++FT+NA+VGKTLPF+S Y G+GYETS   I TPG+YP+T  N+D+   +  + KKI+ + +PIDI+    +S R   G + KLA   + A+YT + Y     G G +FR
Sbjct:  204 GLKHDITEWLPGGKLIPVNISLQAGYTKLNSSIAFNVDPEQGADVENNYDPSTWNGQKAALRTNAFTVNALVGKTLPFISLYGGIGYETSSLVIDTPGSYPVTVPNEDFPDQSS-QPKKIKKVDNPIDISYDKNSSFRGLVGTRIKLAFFNVTASYTLSKYPVGQVGVGFSFR 375          
BLAST of mRNA_M-pyrifera_M_contig85790.20003.1 vs. uniprot
Match: A0A6I7QEU6_9BACT (Uncharacterized protein n=1 Tax=Balneolaceae bacterium TaxID=2053516 RepID=A0A6I7QEU6_9BACT)

HSP 1 Score: 171 bits (432), Expect = 9.010e-49
Identity = 83/178 (46.63%), Postives = 119/178 (66.85%), Query Frame = 1
Query:    1 GVKHGLNQWIPGGKLLPVDISLQAGFTQLTLTTPFDVNPESGSDIKNDFD--PSTWDNQSLDFEASSFTINAIVGKTLPFVSAYVGLGYETSETSIATPGAYPITSVNDDYN---GTTETRTKKIESIVDPIDITIGGENSVRAFGGVQFKLAILKIFANYTAATYSSFNAGFGITFR 519
            GVKHGLNQW+PGG LLPVD+S+Q G+T+L+     ++ PE G DI N F   PS WD+Q +DFEA+ FT N +VGK  P ++ + G+G++TS+ S  +PG+YPIT  N D++    + ETR + IE I DPID++   EN+V A  G + +L  + +  +YT + Y  +N G GI+FR
Sbjct:  198 GVKHGLNQWLPGGALLPVDLSVQVGYTKLSSNFGLELRPEEGEDIYNPFSGTPSLWDDQKIDFEATGFTGNLLVGKNFPMLAVFGGVGFQTSKVSFLSPGSYPITGFNPDFDFMDQSEETRQRIIEQIDDPIDVSFNAENTVHALAGFRVRLGFVALSGSYTLSNYPVYNLGVGISFR 375          
BLAST of mRNA_M-pyrifera_M_contig85790.20003.1 vs. uniprot
Match: A0A6M1T6P2_9BACT (Uncharacterized protein n=1 Tax=Aliifodinibius halophilus TaxID=1736908 RepID=A0A6M1T6P2_9BACT)

HSP 1 Score: 164 bits (416), Expect = 2.130e-46
Identity = 82/178 (46.07%), Postives = 114/178 (64.04%), Query Frame = 1
Query:    1 GVKHGLNQWIPGGKLLPVDISLQAGFTQLTLTTPFDVNPES----GSDIKNDFDPSTWDNQSLDFEASSFTINAIVGKTLPFVSAYVGLGYETSETSIATPGAYPITSVNDDY-NGTTETRTKKIESIVDPIDITIGGENSVRAFGGVQFKLAILKIFANYTAATYSSFNAGFGITFR 519
            G KHGLNQW+PGG +LPV++SL  G+T + + + FDV  E       DIKN +  STWD Q +     +++INA+VGKTLP +S Y G+GYE S  +  TPG+YP    N DY N     +   I+++ +P+DI+I G+N  RA  G +F+ AI  I  +YT + YSS+N GFGI+FR
Sbjct:  197 GAKHGLNQWLPGGGMLPVNLSLMFGYTNMEVGSGFDVTAEDLIEGRQDIKNTYPTSTWDGQKITMNTDAWSINALVGKTLPMISIYGGIGYEASTFNAKTPGSYPTVEGNPDYINDPNNNKKFIIKAVEEPLDISIEGDNGFRALAGFRFRFAIFHISGSYTLSNYSSYNVGFGISFR 374          
BLAST of mRNA_M-pyrifera_M_contig85790.20003.1 vs. uniprot
Match: A0A5D3YR75_9BACT (Uncharacterized protein n=1 Tax=Fodinibius salinus TaxID=860790 RepID=A0A5D3YR75_9BACT)

HSP 1 Score: 160 bits (404), Expect = 1.290e-44
Identity = 79/181 (43.65%), Postives = 118/181 (65.19%), Query Frame = 1
Query:    1 GVKHGLNQWIPGGKLLPVDISLQAGFTQLTLTTPFDVNPES----GSDIKNDFDP--STWDNQSLDFEASSFTINAIVGKTLPFVSAYVGLGYETSETSIATPGAYPITSVNDDYNGTTETRTK--KIESIVDPIDITIGGENSVRAFGGVQFKLAILKIFANYTAATYSSFNAGFGITFR 519
            GVKHGLNQW+PGG  +PVD+S+  G+T+  + + F +  E      +++KN + P  STWD Q +D +  +FT NA+VGKTLPF+S Y G+G E+S  SI+TPG+YP    N ++  +  T  +  ++E++ +PIDI + G NS  A  G + KL+++ I  +Y  A YS+FN G GI+FR
Sbjct:  194 GVKHGLNQWLPGGNRIPVDLSIMVGYTKQNVFSGFRITGEDVVGGRNNVKNPYTPQSSTWDGQKVDVDTKAFTANALVGKTLPFISVYGGIGLESSTVSISTPGSYPTVGANPNFQPSNPTAAEPLRVETLDEPIDIELDGSNSFHALAGFRIKLSVIHISGSYKLADYSTFNLGAGISFR 374          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig85790.20003.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2D8CCS5_9BACT6.080e-6256.74Uncharacterized protein n=2 Tax=Balneolaceae TaxID... [more]
A0A2D9FT35_9BACT3.910e-5955.68Uncharacterized protein n=1 Tax=Balneola sp. TaxID... [more]
A0A316TWW8_9BACT2.000e-5451.69Uncharacterized protein n=1 Tax=Rhodohalobacter mu... [more]
A0A1M5GXR4_9BACT3.790e-5452.02Uncharacterized protein n=1 Tax=Aliifodinibius ros... [more]
UPI00083F69D62.990e-5350.00hypothetical protein n=1 Tax=Rhodohalobacter halop... [more]
A0A2N0VKV8_9BACT3.330e-5248.31Uncharacterized protein n=1 Tax=Rhodohalobacter ba... [more]
A0A521B6J8_9BACT2.280e-4946.82Uncharacterized protein n=1 Tax=Aliifodinibius sed... [more]
A0A6I7QEU6_9BACT9.010e-4946.63Uncharacterized protein n=1 Tax=Balneolaceae bacte... [more]
A0A6M1T6P2_9BACT2.130e-4646.07Uncharacterized protein n=1 Tax=Aliifodinibius hal... [more]
A0A5D3YR75_9BACT1.290e-4443.65Uncharacterized protein n=1 Tax=Fodinibius salinus... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig85790contigM-pyrifera_M_contig85790:336..857 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score127.1
Seed ortholog evalue8e-27
Seed eggNOG ortholog1121930.AQXG01000001_gene1572
Hectar predicted targeting categoryother localisation
EggNOG OGs1IUG2@117747,28J2U@1,2Z8Z5@2,4NHYM@976
Best tax levelSphingobacteriia
Best eggNOG OGNA|NA|NA
Exons1
Model size522
Cds size522
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816768.1521327-CDS-M-pyrifera_M_contig85790:335..8571622816768.1521327-CDS-M-pyrifera_M_contig85790:335..857Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig85790 336..857 -
1692278166.9015746-CDS-M-pyrifera_M_contig85790:335..8571692278166.9015746-CDS-M-pyrifera_M_contig85790:335..857Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig85790 336..857 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig85790.20003.1prot_M-pyrifera_M_contig85790.20003.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig85790 336..857 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig85790.20003.1

>prot_M-pyrifera_M_contig85790.20003.1 ID=prot_M-pyrifera_M_contig85790.20003.1|Name=mRNA_M-pyrifera_M_contig85790.20003.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=174bp
GVKHGLNQWIPGGKLLPVDISLQAGFTQLTLTTPFDVNPESGSDIKNDFD
PSTWDNQSLDFEASSFTINAIVGKTLPFVSAYVGLGYETSETSIATPGAY
PITSVNDDYNGTTETRTKKIESIVDPIDITIGGENSVRAFGGVQFKLAIL
KIFANYTAATYSSFNAGFGITFR*
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mRNA from alignment at M-pyrifera_M_contig85790:336..857-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig85790.20003.1 ID=mRNA_M-pyrifera_M_contig85790.20003.1|Name=mRNA_M-pyrifera_M_contig85790.20003.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=522bp|location=Sequence derived from alignment at M-pyrifera_M_contig85790:336..857- (Macrocystis pyrifera P11B4 male)
GGGGTAAAGCATGGTTTAAACCAATGGATACCTGGTGGAAAATTGCTACC TGTCGATATTTCTCTTCAGGCGGGTTTTACACAATTAACACTTACAACAC CATTCGATGTAAACCCAGAATCGGGTTCTGATATAAAAAACGATTTTGAT CCAAGCACTTGGGATAATCAAAGTCTGGATTTTGAAGCTAGCTCTTTTAC AATAAATGCAATTGTTGGTAAAACTCTTCCATTTGTCAGCGCTTATGTTG GTTTAGGGTATGAAACCTCTGAGACTTCGATTGCCACCCCTGGTGCGTAT CCTATTACATCAGTTAATGATGACTATAATGGTACTACAGAAACACGAAC TAAGAAAATTGAATCTATTGTTGATCCAATTGATATCACAATTGGAGGAG AGAACTCGGTGAGAGCTTTTGGTGGTGTTCAGTTTAAGTTAGCTATTCTA AAAATCTTTGCAAATTATACTGCAGCAACATATTCATCATTTAATGCCGG ATTTGGAATCACTTTCCGGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig85790:336..857-

>mRNA_M-pyrifera_M_contig85790.20003.1 ID=mRNA_M-pyrifera_M_contig85790.20003.1|Name=mRNA_M-pyrifera_M_contig85790.20003.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1044bp|location=Sequence derived from alignment at M-pyrifera_M_contig85790:336..857- (Macrocystis pyrifera P11B4 male)
GGGGTAAAGCATGGTTTAAACCAATGGATACCTGGTGGAAAATTGCTACC
TGTCGATATTTCTCTTCAGGCGGGTTTTACACAATTAACACTTACAACAC
CATTCGATGTAAACCCAGAATCGGGTTCTGATATAAAAAACGATTTTGAT
CCAAGCACTTGGGATAATCAAAGTCTGGATTTTGAAGCTAGCTCTTTTAC
AATAAATGCAATTGTTGGTAAAACTCTTCCATTTGTCAGCGCTTATGTTG
GTTTAGGGTATGAAACCTCTGAGACTTCGATTGCCACCCCTGGTGCGTAT
CCTATTACATCAGTTAATGATGACTATAATGGTACTACAGAAACACGAAC
TAAGAAAATTGAATCTATTGTTGATCCAATTGATATCACAATTGGAGGAG
AGAACTCGGTGAGAGCTTTTGGTGGTGTTCAGTTTAAGTTAGCTATTCTA
AAAATCTTTGCAAATTATACTGCAGCAACATATTCATCATTTAATGCCGG
ATTTGGAATCACTTTCCGGTAAGGGGTAAAGCATGGTTTAAACCAATGGA
TACCTGGTGGAAAATTGCTACCTGTCGATATTTCTCTTCAGGCGGGTTTT
ACACAATTAACACTTACAACACCATTCGATGTAAACCCAGAATCGGGTTC
TGATATAAAAAACGATTTTGATCCAAGCACTTGGGATAATCAAAGTCTGG
ATTTTGAAGCTAGCTCTTTTACAATAAATGCAATTGTTGGTAAAACTCTT
CCATTTGTCAGCGCTTATGTTGGTTTAGGGTATGAAACCTCTGAGACTTC
GATTGCCACCCCTGGTGCGTATCCTATTACATCAGTTAATGATGACTATA
ATGGTACTACAGAAACACGAACTAAGAAAATTGAATCTATTGTTGATCCA
ATTGATATCACAATTGGAGGAGAGAACTCGGTGAGAGCTTTTGGTGGTGT
TCAGTTTAAGTTAGCTATTCTAAAAATCTTTGCAAATTATACTGCAGCAA
CATATTCATCATTTAATGCCGGATTTGGAATCACTTTCCGGTAA
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