mRNA_M-pyrifera_M_contig85604.19959.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig85604.19959.1
Unique NamemRNA_M-pyrifera_M_contig85604.19959.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig85604.19959.1 vs. uniprot
Match: A0A7S1GCI2_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1GCI2_9STRA)

HSP 1 Score: 161 bits (408), Expect = 6.460e-44
Identity = 86/169 (50.89%), Postives = 106/169 (62.72%), Query Frame = 1
Query:    1 MQWQRMHMTGDSPKRRTAHSAG-VDGESLIIFGGWDGMEELGDVVRLSTVGGSGSWSKVETEGTGPSPRHFHSCGVVGRQYL-VFGGYDGSEWRNDLSRLDLETNTWHKIDLVAASERMQKLPGPPRVEHPIPGPRASGSLVVLADGRVLLFGGYDGQTFLDDMWVLHT 501
            M+W R   TG +P  R+AHS   VDG  + +FGGWDG EELGD+    T   +  W+KVET G+ P+PRHFH+   VG   L VFGGYDG  WRND++ LDL+T TW ++                +VE   PG RASGS  VL D R+L+FGGYDG  FLDD+WVLHT
Sbjct:   54 MRWSRCAATGATPIPRSAHSCCMVDGTRMYVFGGWDGQEELGDLHCFDTT--TNFWTKVETTGSPPAPRHFHNAVRVGNNRLYVFGGYDGESWRNDVAVLDLDTLTWTQV----------------KVEGEKPGVRASGSACVLDDDRMLVFGGYDGTDFLDDLWVLHT 204          
BLAST of mRNA_M-pyrifera_M_contig85604.19959.1 vs. uniprot
Match: B6AER9_CRYMR (Kelch motif family protein n=2 Tax=Cryptosporidium TaxID=5806 RepID=B6AER9_CRYMR)

HSP 1 Score: 83.2 bits (204), Expect = 1.720e-15
Identity = 52/170 (30.59%), Postives = 81/170 (47.65%), Query Frame = 1
Query:    1 MQWQRMHMTGDSPKRRTAHSAGVDGESLIIFGGWDGMEELGDVVRLSTVGGSGSWSKVET---EGTGPSPRHFHSCGVVGRQYLVFGGYDGSEWRNDLSRLDLETNTWHKIDLVAASERMQKLPGPPRVEHPIPGPRASGSLVVLADGRVLLFGGYDGQTFLDDMWVLHT 501
            +QW ++   GD P  R+ H+A V G+S+ IFGG+DG   +  +++      S  W+ +     +   P PR  H+  V      +FGG++G+E  ND+   +    TW+ I     S R              P PR S S  V  +  + +FGG DG+ +L+DMW   T
Sbjct:   16 LQWYKVQSYGDKPSPRSLHTANVVGDSMYIFGGYDGRNRINSLLKFDF--NSSKWTDLNISHIKSPLPEPRDRHASAVYKDCIYIFGGHNGTEELNDIWWYNTNNYTWNTISETCTSSRG-------------PSPRHSHSCFVF-EKYIYIFGGNDGR-YLNDMWRFDT 168          
BLAST of mRNA_M-pyrifera_M_contig85604.19959.1 vs. uniprot
Match: A0A7S0YWD0_9CRYP (Hypothetical protein (Fragment) n=1 Tax=Hemiselmis tepida TaxID=464990 RepID=A0A7S0YWD0_9CRYP)

HSP 1 Score: 83.2 bits (204), Expect = 1.800e-15
Identity = 58/175 (33.14%), Postives = 81/175 (46.29%), Query Frame = 1
Query:    7 WQRMHMTGDSPKRRTAHSAG-VDGESLIIFGGWDGMEELGDVVRLSTVGGSGSWSKVETEGTGPSPRHFHSCGVVGRQYLVFGGYDGSEWR----NDLSRLDLETNTWHKIDLVAASERMQKLP-----GPPRVEHPIPGPRASGSLVVLADGRVLLFGGYDGQTFLDDMWVLHT 501
            WQ++ + G  P  R  H+A  +   SL+IFGG        D   L T     +W   + +GTGPSPR +H+  + G +  +FGGY G   R    NDL  LD+  N W  +  V  S   + +      G    E   P PR + S  V+    +L+ GG D   +LDD  VL T
Sbjct:   66 WQKLEVEGKVPLPRWRHTANCISSSSLMIFGGIGEKTRYSDCAILDTESEVPTWHDPQPQGTGPSPRSYHTSTMCGERVYIFGGYGGQGMRRQNFNDLHVLDVPNNIW--VGSVEGSMLPEGITFDAKEGGIVCEGTPPCPRGNHSTSVVERKFLLVMGGRDTGRYLDDCHVLDT 238          
BLAST of mRNA_M-pyrifera_M_contig85604.19959.1 vs. uniprot
Match: A0A5B8MB73_9CHLO (Galactose oxidase n=2 Tax=Chloropicon primus TaxID=1764295 RepID=A0A5B8MB73_9CHLO)

HSP 1 Score: 81.3 bits (199), Expect = 7.710e-15
Identity = 56/174 (32.18%), Postives = 85/174 (48.85%), Query Frame = 1
Query:    1 MQWQRMHMT-GDSP-KRRTAH------SAGVDGESLIIFGGWDGMEELGDVVRLSTVGGSGSWSKVETEGTGPSPRHFHSCGVVGRQYLVFGGYDGSEWRNDLSRLDLETNTWHKIDLVAASERMQKLPGPPRVEHPIPGPRASGSLVVLADGRVLLFGGYDGQTFLDDMWVLH 498
            M W+R   T G+ P   R  H      S+  DG  ++  GG +  + LGD+V L     +G W++ +T G GPSPR FH   V+G     FGG  G+   N++ +LD     W  ++L+ A                 P  R     + L + RVL+FGGYDG  +L+D++V++
Sbjct:    1 MLWKRYQCTYGEPPPSARCGHCVVKVKSSVWDGTLVVFHGGTNDRQFLGDLVVLHLE--NGLWTRPQTTG-GPSPRAFHCGAVIGTSVYYFGGRTGARVHNEVWKLDTNLWEWTCLNLIGAK----------------PDAREKADCIGLDESRVLVFGGYDGIRWLNDVFVIN 155          
BLAST of mRNA_M-pyrifera_M_contig85604.19959.1 vs. uniprot
Match: A0A7S1F0M2_NOCSC (Hypothetical protein (Fragment) n=1 Tax=Noctiluca scintillans TaxID=2966 RepID=A0A7S1F0M2_NOCSC)

HSP 1 Score: 79.3 bits (194), Expect = 2.050e-14
Identity = 47/167 (28.14%), Postives = 83/167 (49.70%), Query Frame = 1
Query:    7 WQRMHMTGDSPKRRTAHS-AGVDGESLIIFGGWDGMEELGDVVRLSTVGGSGSWSKVETEGTGPSPRHFHSC-GVVGRQYLVFGGYDGSEWRNDLSRLDLE--TNTWHKIDLVAASERMQKLPGPPRVEHPIPGPRASGSLVVLADGRVLLFGGYDGQTFLDDMWVL 495
            W  +   G  P  R  H+   +   + ++FGG++    L DV +L   G + +W ++   G  PS R  H+  G+    +++FGGYDG+++ +D+ +L++   T TW     V                  +P  R +  +V L DG V++FGG DG++ L+D++ L
Sbjct:  147 WNHLTNAGTIPSVRAGHTMTALSDNTGVMFGGYNYDNYLNDVFQLQRSGDTATWMQLTDAGNPPSGRSVHAMTGLADGTFVIFGGYDGADYLSDIYQLEVSGTTATWTMFSNVGD----------------VPSNRNALGMVELGDGTVVVFGGNDGESSLNDVYQL 297          
BLAST of mRNA_M-pyrifera_M_contig85604.19959.1 vs. uniprot
Match: A0A2R6WWR8_MARPO (Uncharacterized protein n=2 Tax=Marchantia polymorpha TaxID=3197 RepID=A0A2R6WWR8_MARPO)

HSP 1 Score: 79.0 bits (193), Expect = 4.900e-14
Identity = 54/165 (32.73%), Postives = 80/165 (48.48%), Query Frame = 1
Query:   28 GDSPKRRTAHSAGVDGESLI-IFGGWDGMEELGDVVRLSTVGGSGSWSKVETEGTG------PSPRHFHSCGVVGRQYLVFGGYDGSEWRNDLSRLDLETNTWHKIDLVAASERMQKLPGPPRVEHPIPGPRASGSLVVLADGRVLLFGGYDGQTFLDDMWVLHT 501
            GD P  R+ H+A   G+SL+ +FGG    + L D+V L T   +  W K E  G G      PSPR FH C  +     +FGG  G +   D   LD  T+TW   +L    E              +P PR   +   + +G+++++GG+DG  +L D++VL T
Sbjct:   13 GDPPSLRSGHTAIKVGKSLLAVFGGLVDKKFLNDLVVLDTD--TLQWFKPECTGNGESGHIGPSPRAFHVCVAIDCHLFIFGGRCGRKRLGDFWMLD--TDTWQWAELSGYGE--------------LPPPREFAAGASVGNGKIVIYGGWDGTKWLSDVYVLDT 159          
BLAST of mRNA_M-pyrifera_M_contig85604.19959.1 vs. uniprot
Match: A0A0C3LK96_9AGAM (Uncharacterized protein n=1 Tax=Tulasnella calospora MUT 4182 TaxID=1051891 RepID=A0A0C3LK96_9AGAM)

HSP 1 Score: 74.3 bits (181), Expect = 2.130e-12
Identity = 50/180 (27.78%), Postives = 77/180 (42.78%), Query Frame = 1
Query:   37 PKRRTAHSAGVDGESLIIFGGWDGMEELGDVVRLSTVGGSGS--WSKVETEGTGPSPRHFHSCGVVGRQYLVFGGYDGSEWRNDLSRLDLETNTWHKIDLVAASERMQKLPGPP-------------RVEHPI------------PGPRASGSLVVLADGRVLLFGGYDGQTFLDDMWVL 495
            P  R AH+A +  +++ +FGG +G   L D+  L    G     W +V+T+GT P  R +H+  +VG   +V GG DG E   D+  LDL+   W ++       R+                    +  H +                A      LAD R+ +FGG+DG    DD+W+L
Sbjct:  521 PLPRPAHAAVLYRDTIYVFGGGNGHRALNDLWALDVSVGMDKLKWEQVQTQGTKPGARGYHTANLVGNVMIVLGGSDGRECFPDVWVLDLDKLVWREVKPDKTYRRLSXXXXXXXXXXXXXXXXXXNKYSHELLLFNLASINSRSTTVFARLPFTTLADSRLWVFGGFDGHIVYDDVWIL 700          
BLAST of mRNA_M-pyrifera_M_contig85604.19959.1 vs. uniprot
Match: A3FQ45_CRYPI (BTB domain-containing protein n=5 Tax=Cryptosporidium TaxID=5806 RepID=A3FQ45_CRYPI)

HSP 1 Score: 73.9 bits (180), Expect = 2.710e-12
Identity = 53/166 (31.93%), Postives = 74/166 (44.58%), Query Frame = 1
Query:    1 MQWQRMHMTGDSPKRRTAHSAGVDGESLIIFGGWDGMEELGDVVRLSTVGGSGSWSKVETEGTGPSPRHFHSCGVVGR-QYLVFGGYDGSEWRNDLSRLDLETNTWHKIDLVAASERMQKLPGPPRVEHPIPGPRASGSLVVLADGRVLLFGGYDGQTFLDDMWVL 495
            M W  +      P+ R  H A    + +I FGG  G E +GDV   +         KV   G  PS R  HS  ++     ++FGGYDG    NDL  LD+      ++  +   ER+        +E   P PR   SL  +  G+ LLFGGYDG  +  D ++L
Sbjct:  181 MTWNEIEFENYIPRYRNGHCAISSSKGIIFFGGNTGKEYIGDVSLYNPEKKEFQTPKVF--GVCPSARKGHSLALLDDVSAVMFGGYDGKNRCNDLFILDIS-----ELPSIVRWERI--------IEKNSPSPRQRNSLTTIPGGKCLLFGGYDGNCWKSDTYLL 331          
BLAST of mRNA_M-pyrifera_M_contig85604.19959.1 vs. uniprot
Match: A0A5N6KXJ8_9ROSI (Uncharacterized protein n=1 Tax=Carpinus fangiana TaxID=176857 RepID=A0A5N6KXJ8_9ROSI)

HSP 1 Score: 73.2 bits (178), Expect = 5.830e-12
Identity = 52/164 (31.71%), Postives = 80/164 (48.78%), Query Frame = 1
Query:   25 TGDSPKRRTAHSAGVDGESLIIFGG---WDGMEELGDVVRLSTVGGSGSWSKVETEGTGPSPRHFHSCGVVGRQYLVFGGYDGSEWRNDLSRLDLET-----NTWHKIDLVAASERMQKLPGPPRVEHPIPGPRASGSLVVLADGRVLLFGGYDGQTFLDDMWV 492
            T + P  R  H++ + G + I+FGG    D ++ L D + L     +  WS+    G  PS R+ H+  ++G +  VFGG     + NDL   DL       N W +I +  +S+      GPP  + P   P  +   V+    R+LLFGG DG T+ +D+WV
Sbjct:  167 TAEGPGPRVGHASLLVGNAYIVFGGDTKVDELDVLDDTLYLLNTS-TKHWSRALPAGPRPSGRYGHTLNILGSRIYVFGGQVEGYFFNDLVSFDLNALQQPNNRW-EILIQNSSDG-----GPPTGQVP---PARTNHTVITHQERLLLFGGTDGNTWFNDVWV 320          
BLAST of mRNA_M-pyrifera_M_contig85604.19959.1 vs. uniprot
Match: I7M609_TETTS (Kelch motif protein n=1 Tax=Tetrahymena thermophila (strain SB210) TaxID=312017 RepID=I7M609_TETTS)

HSP 1 Score: 72.8 bits (177), Expect = 6.190e-12
Identity = 52/172 (30.23%), Postives = 80/172 (46.51%), Query Frame = 1
Query:    4 QWQRMHMTGDSPKRRTAHSAGVDGESLIIFGGW------DGMEELGDVVRLSTVGGSGSWSKVETEGTGPSPRHFHSCGVVGRQYLVFGGYD--GSEWRNDLSRLDLETNTWHKIDLVAASERMQKLPGPPRVEHPIPGPRASGSLVVLADGRVLLFGGYDGQTFLDDMWVL 495
            QW +  + G  P  R  HSA + G +++IFGG        G   L D   L     S  W K +  GT P+PR+ HS  + G + ++FGG    G  +R DL  LD  T TW++    + S                P  R   S  ++   ++L+FGG++G  F +D+++L
Sbjct:   82 QWAQPLIEGVPPCPRGGHSATLSGATIVIFGGHYYAGKQKGYVYLNDTYILDV--NSNRWHKPKISGTPPAPRYNHSAILAGSRIIIFGGKGEKGKVYR-DLHALDPVTTTWYQGPEGSGS----------------PSARFGHSANLVGGSKMLIFGGWNGSDFFNDLYLL 234          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig85604.19959.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S1GCI2_9STRA6.460e-4450.89Hypothetical protein (Fragment) n=1 Tax=Bicosoecid... [more]
B6AER9_CRYMR1.720e-1530.59Kelch motif family protein n=2 Tax=Cryptosporidium... [more]
A0A7S0YWD0_9CRYP1.800e-1533.14Hypothetical protein (Fragment) n=1 Tax=Hemiselmis... [more]
A0A5B8MB73_9CHLO7.710e-1532.18Galactose oxidase n=2 Tax=Chloropicon primus TaxID... [more]
A0A7S1F0M2_NOCSC2.050e-1428.14Hypothetical protein (Fragment) n=1 Tax=Noctiluca ... [more]
A0A2R6WWR8_MARPO4.900e-1432.73Uncharacterized protein n=2 Tax=Marchantia polymor... [more]
A0A0C3LK96_9AGAM2.130e-1227.78Uncharacterized protein n=1 Tax=Tulasnella calospo... [more]
A3FQ45_CRYPI2.710e-1231.93BTB domain-containing protein n=5 Tax=Cryptosporid... [more]
A0A5N6KXJ8_9ROSI5.830e-1231.71Uncharacterized protein n=1 Tax=Carpinus fangiana ... [more]
I7M609_TETTS6.190e-1230.23Kelch motif protein n=1 Tax=Tetrahymena thermophil... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig85604contigM-pyrifera_M_contig85604:320..823 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score102.8
Seed ortholog evalue1.6e-19
Seed eggNOG ortholog67593.Physo134069
Preferred namelztr1
KEGG koko:K12115,ko:K12116,ko:K12117
KEGG Pathwayko04712,map04712
Hectar predicted targeting categoryother localisation
GOsGO:0003008,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005737,GO:0005829,GO:0007600,GO:0008150,GO:0015030,GO:0016604,GO:0019233,GO:0031974,GO:0031981,GO:0032501,GO:0042752,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044451,GO:0044464,GO:0050789,GO:0050877,GO:0065007,GO:0070013
EggNOG free text desc.Kelch
EggNOG OGs2CMFB@1,2QQ74@2759,3Q9Z7@4776
COG Functional cat.S
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04121
Exons1
Model size504
Cds size504
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816765.9057767-CDS-M-pyrifera_M_contig85604:319..8231622816765.9057767-CDS-M-pyrifera_M_contig85604:319..823Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig85604 320..823 -
1692278165.4393039-CDS-M-pyrifera_M_contig85604:319..8231692278165.4393039-CDS-M-pyrifera_M_contig85604:319..823Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig85604 320..823 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig85604.19959.1prot_M-pyrifera_M_contig85604.19959.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig85604 320..823 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig85604.19959.1

>prot_M-pyrifera_M_contig85604.19959.1 ID=prot_M-pyrifera_M_contig85604.19959.1|Name=mRNA_M-pyrifera_M_contig85604.19959.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=168bp
MQWQRMHMTGDSPKRRTAHSAGVDGESLIIFGGWDGMEELGDVVRLSTVG
GSGSWSKVETEGTGPSPRHFHSCGVVGRQYLVFGGYDGSEWRNDLSRLDL
ETNTWHKIDLVAASERMQKLPGPPRVEHPIPGPRASGSLVVLADGRVLLF
GGYDGQTFLDDMWVLHTP
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mRNA from alignment at M-pyrifera_M_contig85604:320..823-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig85604.19959.1 ID=mRNA_M-pyrifera_M_contig85604.19959.1|Name=mRNA_M-pyrifera_M_contig85604.19959.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=504bp|location=Sequence derived from alignment at M-pyrifera_M_contig85604:320..823- (Macrocystis pyrifera P11B4 male)
ATGCAATGGCAGCGTATGCATATGACAGGGGACTCTCCAAAACGTCGCAC AGCACACTCTGCGGGGGTAGATGGGGAAAGCTTAATCATATTCGGTGGAT GGGATGGAATGGAGGAATTAGGAGATGTCGTGCGATTGAGTACTGTTGGA GGTAGTGGATCATGGAGCAAAGTAGAGACAGAAGGCACAGGGCCATCTCC AAGGCACTTTCATTCTTGTGGAGTTGTTGGACGACAGTACCTGGTATTTG GGGGATATGATGGGTCTGAGTGGCGAAATGATTTGAGTCGCTTAGACTTG GAGACAAACACATGGCATAAAATTGACCTAGTAGCCGCTTCGGAGAGAAT GCAAAAGTTGCCCGGACCACCACGAGTAGAACATCCAATCCCTGGTCCTC GTGCTTCTGGTTCTCTTGTCGTATTGGCAGATGGACGTGTGTTGCTCTTT GGTGGTTATGATGGACAAACGTTCTTGGATGATATGTGGGTGTTGCATAC TCCA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig85604:320..823-

>mRNA_M-pyrifera_M_contig85604.19959.1 ID=mRNA_M-pyrifera_M_contig85604.19959.1|Name=mRNA_M-pyrifera_M_contig85604.19959.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1008bp|location=Sequence derived from alignment at M-pyrifera_M_contig85604:320..823- (Macrocystis pyrifera P11B4 male)
ATGCAATGGCAGCGTATGCATATGACAGGGGACTCTCCAAAACGTCGCAC
AGCACACTCTGCGGGGGTAGATGGGGAAAGCTTAATCATATTCGGTGGAT
GGGATGGAATGGAGGAATTAGGAGATGTCGTGCGATTGAGTACTGTTGGA
GGTAGTGGATCATGGAGCAAAGTAGAGACAGAAGGCACAGGGCCATCTCC
AAGGCACTTTCATTCTTGTGGAGTTGTTGGACGACAGTACCTGGTATTTG
GGGGATATGATGGGTCTGAGTGGCGAAATGATTTGAGTCGCTTAGACTTG
GAGACAAACACATGGCATAAAATTGACCTAGTAGCCGCTTCGGAGAGAAT
GCAAAAGTTGCCCGGACCACCACGAGTAGAACATCCAATCCCTGGTCCTC
GTGCTTCTGGTTCTCTTGTCGTATTGGCAGATGGACGTGTGTTGCTCTTT
GGTGGTTATGATGGACAAACGTTCTTGGATGATATGTGGGTGTTGCATAC
TCCAATGCAATGGCAGCGTATGCATATGACAGGGGACTCTCCAAAACGTC
GCACAGCACACTCTGCGGGGGTAGATGGGGAAAGCTTAATCATATTCGGT
GGATGGGATGGAATGGAGGAATTAGGAGATGTCGTGCGATTGAGTACTGT
TGGAGGTAGTGGATCATGGAGCAAAGTAGAGACAGAAGGCACAGGGCCAT
CTCCAAGGCACTTTCATTCTTGTGGAGTTGTTGGACGACAGTACCTGGTA
TTTGGGGGATATGATGGGTCTGAGTGGCGAAATGATTTGAGTCGCTTAGA
CTTGGAGACAAACACATGGCATAAAATTGACCTAGTAGCCGCTTCGGAGA
GAATGCAAAAGTTGCCCGGACCACCACGAGTAGAACATCCAATCCCTGGT
CCTCGTGCTTCTGGTTCTCTTGTCGTATTGGCAGATGGACGTGTGTTGCT
CTTTGGTGGTTATGATGGACAAACGTTCTTGGATGATATGTGGGTGTTGC
ATACTCCA
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