mRNA_M-pyrifera_M_contig85289.19879.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig85289.19879.1
Unique NamemRNA_M-pyrifera_M_contig85289.19879.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig85289.19879.1 vs. uniprot
Match: D8LTZ2_ECTSI (DNA polymerase alpha subunit B n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LTZ2_ECTSI)

HSP 1 Score: 223 bits (569), Expect = 7.790e-67
Identity = 111/129 (86.05%), Postives = 116/129 (89.92%), Query Frame = 1 
Query:    4 GSRRDSGGQRVHLDLRELQSFALFPGQAVAVQGVNGSGGRMVARGIIDGVPRPLPSSAPSELARVQHETELGGGRPLSIFAVAGPFTTSDSLVYEPLNDLLGAVKVAKPDVVVLMGPFVDAEHPKVGRG 390
            GSRRDS GQR HLDLRELQSFALFPGQ VAVQGVNGSGGRMVARGIIDGVPRPLPSS PS+L R QH  ELGGG+ LSIFA AGPFTTSDSLVYEPLNDLLGAV+ A+PDVVVLMGPFVD+EHPKV  G
Sbjct:  296 GSRRDSSGQRAHLDLRELQSFALFPGQVVAVQGVNGSGGRMVARGIIDGVPRPLPSSRPSDLLRAQHGAELGGGKALSIFAAAGPFTTSDSLVYEPLNDLLGAVRAARPDVVVLMGPFVDSEHPKVASG 424
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BLAST of mRNA_M-pyrifera_M_contig85289.19879.1 vs. uniprot
Match: W7TPC9_9STRA (DNA polymerase alpha subunit B n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TPC9_9STRA)

HSP 1 Score: 148 bits (374), Expect = 4.990e-39
Identity = 74/132 (56.06%), Postives = 93/132 (70.45%), Query Frame = 1 
Query:    4 GSRRDSGGQRVHLDLRELQSFALFPGQAVAVQGVNGSGGRMVARGIIDGVPRPLPSSAPSELARVQHETELGGGRPLSIFAVAGPFTTSDSLVYEPLNDLLGAVKVAKPDVVVLMGPFVDAEHPKVGRGRGE 399
            GSRRDSGG RV L+L +L +F+LFPGQ VAV+GVN SG +MV R II+G+PRP  +S   ++   QH++   GG  LS+   AGP+TT+DSL YEPL DLL  +   +PDV VL GPFVDA HP+V  G  E
Sbjct:  302 GSRRDSGGFRVKLELGQLPAFSLFPGQVVAVEGVNSSGFKMVVRRIIEGIPRPFLTSPAKKMLEFQHDSLYQGGGALSLLVAAGPYTTTDSLEYEPLEDLLKVIAARQPDVAVLCGPFVDANHPQVASGETE 433
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BLAST of mRNA_M-pyrifera_M_contig85289.19879.1 vs. uniprot
Match: A0A812STA5_SYMMI (DNA polymerase alpha subunit B (Fragment) n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812STA5_SYMMI)

HSP 1 Score: 139 bits (350), Expect = 8.740e-37
Identity = 70/129 (54.26%), Postives = 87/129 (67.44%), Query Frame = 1 
Query:    4 GSRRDSGGQRVHLDLRELQSFALFPGQAVAVQGVNGSGGRMVARGIIDGVPRPLPSSAPSELARVQHETELGGGRPLSIFAVAGPFTTSDSLVYEPLNDLLGAVKVAKPDVVVLMGPFVDAEHPKVGRG 390
            GSR  S  +R HLDL+EL  +ALFPGQ V V+G+N SG RMVA+ I++GVPRPL  S PS L    H     GG  L++   AGPFTT+D+L YEPL +LL  V   KPDV++L+GPFVD+  P V  G
Sbjct:  196 GSRSGSSRRRAHLDLQELPQYALFPGQIVLVEGINASGRRMVAKRILEGVPRPLSQSPPSRLLEYHHSRLHQGGGALNVIVAAGPFTTTDNLDYEPLEELLRLVAEDKPDVLILIGPFVDSSQPLVAEG 324
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BLAST of mRNA_M-pyrifera_M_contig85289.19879.1 vs. uniprot
Match: A0A7S2XQT5_9STRA (DNA polymerase alpha subunit B n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2XQT5_9STRA)

HSP 1 Score: 121 bits (303), Expect = 3.120e-29
Identity = 65/134 (48.51%), Postives = 93/134 (69.40%), Query Frame = 1 
Query:    4 GSRRDSGGQRVHLDLRELQS-FALFPGQAVAVQGVNGSGGRMVARGIIDGVPR-PLPSSAPSELARVQHETELGGGRPLSIFAVAGPFTTSDSLVYEPLNDLLGAVKVAKPDVVVLMGPFVDAEHPKVGRGRGE 399
            GS   SGG+RVHLD+ +L + F+LFPGQ VAV+G+N  GG+M A+ I +G    PL +SA  EL ++ H+T+   G+ L I +++GP+TTSD+L YEPL  +L  V + KPDVV++ GPFVD  H  + +G+ E
Sbjct:  300 GSHDFSGGRRVHLDVSKLTTGFSLFPGQIVAVEGINAYGGKMTAQRICEGSSHEPLKTSA-KELLKLHHDTDHQNGQGLKIMSMSGPYTTSDNLDYEPLTAILEEVLMQKPDVVIMTGPFVDVTHKSIQQGKVE 432
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BLAST of mRNA_M-pyrifera_M_contig85289.19879.1 vs. uniprot
Match: A0A7S0JQP6_CAFRO (Hypothetical protein n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A7S0JQP6_CAFRO)

HSP 1 Score: 119 bits (297), Expect = 4.260e-29
Identity = 62/132 (46.97%), Postives = 83/132 (62.88%), Query Frame = 1 
Query:    4 GSRRDSGGQRVHLDLRELQSFALFPGQAVAVQGVNGSGGRMVARGIIDGVPRPLPSSAPSELARVQHETELGGGRPLSIFAVAGPFTTSDSLVYEPLNDLLGAVKVAKP--DVVVLMGPFVDAEHPKVGRGR 393
            GSRR SGG RV LDL + ++FALFPGQ VA +G+N +G RMV + +  G   PLP S  + L          G  P+ ++A AGP++ +  L Y+PL+DLL     ++P  DV+VL GPFVDA+HP V  GR
Sbjct:  259 GSRRTSGGDRVQLDLMDCKAFALFPGQVVAARGMNATGSRMVVQSVAHGARLPLPRSPVAYLEAEAARCAANGSAPMRVWAAAGPYSLASDLEYQPLDDLLIEATQSEPPPDVLVLSGPFVDADHPHVRAGR 390
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BLAST of mRNA_M-pyrifera_M_contig85289.19879.1 vs. uniprot
Match: B8BYS5_THAPS (DNA polymerase alpha subunit B n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8BYS5_THAPS)

HSP 1 Score: 120 bits (302), Expect = 4.280e-29
Identity = 61/133 (45.86%), Postives = 83/133 (62.41%), Query Frame = 1 
Query:    4 GSRRDSGGQRVHLDLREL-------QSFALFPGQAVAVQGVNGSGGRMVARGIIDGVPRPLPSSAPSELARVQHETELGGGRPLSIFAVAGPFTTSDSLVYEPLNDLLGAVKVAKPDVVVLMGPFVDAEHPKV 381
            GSR+DS G R+HLDL  L       QS++ FPGQ VAV+G+N SG  M A  + +GVP P+  S   EL +  +  +   G PLSI ++ GPFTT D+L Y+PL D++  +   +PDVV++ GPFVD   P V
Sbjct:  327 GSRKDSSGSRIHLDLNSLVTNNDNNQSYSFFPGQIVAVEGINSSGRTMQASRLYEGVPPPVEKSTAKELIKYNYGPDGQNGMPLSIVSMCGPFTTKDNLEYDPLVDMVMKISEDQPDVVIMCGPFVDGRQPLV 459
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BLAST of mRNA_M-pyrifera_M_contig85289.19879.1 vs. uniprot
Match: A0A5A8DV68_CAFRO (DNA polymerase alpha subunit B n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DV68_CAFRO)

HSP 1 Score: 119 bits (297), Expect = 1.460e-28
Identity = 62/132 (46.97%), Postives = 83/132 (62.88%), Query Frame = 1 
Query:    4 GSRRDSGGQRVHLDLRELQSFALFPGQAVAVQGVNGSGGRMVARGIIDGVPRPLPSSAPSELARVQHETELGGGRPLSIFAVAGPFTTSDSLVYEPLNDLLGAVKVAKP--DVVVLMGPFVDAEHPKVGRGR 393
            GSRR SGG RV LDL + ++FALFPGQ VA +G+N +G RMV + +  G   PLP S  + L          G  P+ ++A AGP++ +  L Y+PL+DLL     ++P  DV+VL GPFVDA+HP V  GR
Sbjct:  269 GSRRTSGGDRVQLDLMDCKAFALFPGQVVAARGMNATGSRMVVQSVAHGARLPLPRSPVAYLEAEAARCAANGSAPMRVWAAAGPYSLASDLEYQPLDDLLIEATQSEPPPDVLVLSGPFVDADHPHVRAGR 400
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BLAST of mRNA_M-pyrifera_M_contig85289.19879.1 vs. uniprot
Match: A0A5A8C4S8_CAFRO (DNA polymerase alpha subunit B n=2 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8C4S8_CAFRO)

HSP 1 Score: 119 bits (297), Expect = 2.060e-28
Identity = 62/132 (46.97%), Postives = 83/132 (62.88%), Query Frame = 1 
Query:    4 GSRRDSGGQRVHLDLRELQSFALFPGQAVAVQGVNGSGGRMVARGIIDGVPRPLPSSAPSELARVQHETELGGGRPLSIFAVAGPFTTSDSLVYEPLNDLLGAVKVAKP--DVVVLMGPFVDAEHPKVGRGR 393
            GSRR SGG RV LDL + ++FALFPGQ VA +G+N +G RMV + +  G   PLP S  + L          G  P+ ++A AGP++ +  L Y+PL+DLL     ++P  DV+VL GPFVDA+HP V  GR
Sbjct:  358 GSRRTSGGDRVQLDLMDCKAFALFPGQVVAARGMNATGSRMVVQSVAHGARLPLPRSPVAYLEAEAARCAANGSAPMRVWAAAGPYSLASDLEYQPLDDLLIEATQSEPPPDVLVLSGPFVDADHPHVRAGR 489
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BLAST of mRNA_M-pyrifera_M_contig85289.19879.1 vs. uniprot
Match: A0A836CA88_9STRA (DNA polymerase alpha subunit B n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CA88_9STRA)

HSP 1 Score: 118 bits (295), Expect = 3.730e-28
Identity = 65/129 (50.39%), Postives = 75/129 (58.14%), Query Frame = 1 
Query:    4 GSRRDSGGQRVHLDLRELQSFALFPGQAVAVQGVNGSGGRMVARGIIDGVPRPLPSSAPSELARVQHETELGGGRPLSIFAVAGPFTTSDSLVYEPLNDLLGAVKVAKPDVVVLMGPFVDAEHPKVGRG 390
            GS RDSGG R  L L  L SFALFPGQ VA +G+N  G RM  RG++ G P P     P+                      AGP+TT D+L YEPLNDLL  V+  +PDVVVLMGPFVDAEHP +  G
Sbjct:  318 GSWRDSGGARAALALDALPSFALFPGQIVAARGINSGGMRMAVRGLLPGSPAP-----PAXXXXXXXXXXXXXXXXXXXXVAAGPYTTDDNLEYEPLNDLLAHVQETRPDVVVLMGPFVDAEHPLIAAG 441
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BLAST of mRNA_M-pyrifera_M_contig85289.19879.1 vs. uniprot
Match: A0A843USD7_COLES (DNA polymerase alpha subunit B n=1 Tax=Colocasia esculenta TaxID=4460 RepID=A0A843USD7_COLES)

HSP 1 Score: 117 bits (293), Expect = 6.090e-28
Identity = 61/134 (45.52%), Postives = 86/134 (64.18%), Query Frame = 1 
Query:    4 GSRRDSGGQRVHLDLRELQSFALFPGQAVAVQGVNGSGGRMVARGIIDGVPRPLPSSAPSELARVQHETELGGGRP-----LSIFAVAGPFTTSDSLVYEPLNDLLGAVKVAKPDVVVLMGPFVDAEHPKVGRG 390
            GS   SGGQRV LDL++L  F+LFPGQ + V+G N SG  ++A  + D +P  + SS PS   +   + +  G  P     LS+   AGPFTT+D+L++EPLN++L      +P +++LMGPFVD+EHP V RG
Sbjct:  274 GSVEHSGGQRVRLDLQKLVEFSLFPGQVIVVEGQNPSGHCLIASKVTDSLPLSIHSSVPSAKRQAIGQEDQSGVPPYTPKVLSMVIAAGPFTTTDNLLFEPLNEILAYASRKQPQLLILMGPFVDSEHPDVKRG 407
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig85289.19879.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LTZ2_ECTSI7.790e-6786.05DNA polymerase alpha subunit B n=2 Tax=Ectocarpus ... [more]
W7TPC9_9STRA4.990e-3956.06DNA polymerase alpha subunit B n=2 Tax=Monodopsida... [more]
A0A812STA5_SYMMI8.740e-3754.26DNA polymerase alpha subunit B (Fragment) n=1 Tax=... [more]
A0A7S2XQT5_9STRA3.120e-2948.51DNA polymerase alpha subunit B n=1 Tax=Attheya sep... [more]
A0A7S0JQP6_CAFRO4.260e-2946.97Hypothetical protein n=1 Tax=Cafeteria roenbergens... [more]
B8BYS5_THAPS4.280e-2945.86DNA polymerase alpha subunit B n=1 Tax=Thalassiosi... [more]
A0A5A8DV68_CAFRO1.460e-2846.97DNA polymerase alpha subunit B n=1 Tax=Cafeteria r... [more]
A0A5A8C4S8_CAFRO2.060e-2846.97DNA polymerase alpha subunit B n=2 Tax=Cafeteria r... [more]
A0A836CA88_9STRA3.730e-2850.39DNA polymerase alpha subunit B n=1 Tax=Tribonema m... [more]
A0A843USD7_COLES6.090e-2845.52DNA polymerase alpha subunit B n=1 Tax=Colocasia e... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig85289contigM-pyrifera_M_contig85289:30..446 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score224.2
Seed ortholog evalue3.9e-56
Seed eggNOG ortholog2880.D8LTZ2
Preferred namePOLA2
KEGG rclassRC02795
KEGG koko:K02321
KEGG ReactionR00375,R00376,R00377,R00378
KEGG Pathwayko00230,ko00240,ko01100,ko03030,map00230,map00240,map01100,map03030
KEGG ModuleM00261
Hectar predicted targeting categoryother localisation
GOsGO:0000082,GO:0000228,GO:0000278,GO:0000428,GO:0000723,GO:0000785,GO:0000790,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005635,GO:0005654,GO:0005657,GO:0005658,GO:0005694,GO:0005737,GO:0005829,GO:0006139,GO:0006259,GO:0006260,GO:0006261,GO:0006269,GO:0006270,GO:0006271,GO:0006273,GO:0006725,GO:0006807,GO:0006996,GO:0007049,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0012505,GO:0016043,GO:0016070,GO:0016233,GO:0018130,GO:0019438,GO:0019725,GO:0022402,GO:0022616,GO:0030880,GO:0030894,GO:0031967,GO:0031974,GO:0031975,GO:0031981,GO:0032200,GO:0032201,GO:0032774,GO:0032991,GO:0032993,GO:0033260,GO:0034641,GO:0034645,GO:0034654,GO:0042592,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043596,GO:0043601,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044444,GO:0044446,GO:0044454,GO:0044464,GO:0044770,GO:0044772,GO:0044786,GO:0044843,GO:0046483,GO:0046982,GO:0046983,GO:0051276,GO:0055029,GO:0060249,GO:0061695,GO:0065007,GO:0065008,GO:0070013,GO:0071704,GO:0071840,GO:0090304,GO:1901360,GO:1901362,GO:1901576,GO:1902292,GO:1902295,GO:1902315,GO:1902318,GO:1902494,GO:1902969,GO:1902975,GO:1902981,GO:1903047,GO:1990234
EggNOG free text desc.DNA-directed DNA polymerase activity
EggNOG OGsCOG5214@1,KOG1625@2759
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03032
Exons1
Model size417
Cds size417
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816761.4852126-CDS-M-pyrifera_M_contig85289:29..4461622816761.4852126-CDS-M-pyrifera_M_contig85289:29..446Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig85289 30..446 +
1692278162.9729738-CDS-M-pyrifera_M_contig85289:29..4461692278162.9729738-CDS-M-pyrifera_M_contig85289:29..446Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig85289 30..446 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig85289.19879.1prot_M-pyrifera_M_contig85289.19879.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig85289 30..446 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig85289.19879.1

>prot_M-pyrifera_M_contig85289.19879.1 ID=prot_M-pyrifera_M_contig85289.19879.1|Name=mRNA_M-pyrifera_M_contig85289.19879.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=139bp
KGSRRDSGGQRVHLDLRELQSFALFPGQAVAVQGVNGSGGRMVARGIIDG
VPRPLPSSAPSELARVQHETELGGGRPLSIFAVAGPFTTSDSLVYEPLND
LLGAVKVAKPDVVVLMGPFVDAEHPKVGRGRGEEGSGAE
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mRNA from alignment at M-pyrifera_M_contig85289:30..446+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig85289.19879.1 ID=mRNA_M-pyrifera_M_contig85289.19879.1|Name=mRNA_M-pyrifera_M_contig85289.19879.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=417bp|location=Sequence derived from alignment at M-pyrifera_M_contig85289:30..446+ (Macrocystis pyrifera P11B4 male)
AAGGGCTCCCGTCGCGACAGCGGCGGCCAGCGGGTCCACCTCGACCTCCG
CGAGCTGCAGTCGTTCGCGCTCTTCCCGGGCCAGGCGGTCGCAGTCCAGG
GCGTGAACGGCTCGGGCGGCCGGATGGTGGCGCGCGGGATCATCGACGGG
GTGCCTCGACCCCTGCCTTCCTCCGCGCCCTCCGAGCTGGCGCGGGTGCA
GCATGAGACGGAGCTGGGGGGCGGACGGCCGCTGTCCATCTTTGCGGTGG
CGGGACCATTCACGACGAGCGACAGCTTGGTGTACGAGCCGCTGAACGAT
CTGCTCGGGGCGGTGAAGGTGGCGAAGCCGGACGTGGTGGTGCTCATGGG
GCCCTTTGTCGACGCGGAGCACCCCAAGGTGGGACGGGGGAGGGGAGAGG
AGGGGAGCGGGGCGGAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig85289:30..446+

>mRNA_M-pyrifera_M_contig85289.19879.1 ID=mRNA_M-pyrifera_M_contig85289.19879.1|Name=mRNA_M-pyrifera_M_contig85289.19879.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=834bp|location=Sequence derived from alignment at M-pyrifera_M_contig85289:30..446+ (Macrocystis pyrifera P11B4 male)
AAGGGCTCCCGTCGCGACAGCGGCGGCCAGCGGGTCCACCTCGACCTCCG
CGAGCTGCAGTCGTTCGCGCTCTTCCCGGGCCAGGCGGTCGCAGTCCAGG
GCGTGAACGGCTCGGGCGGCCGGATGGTGGCGCGCGGGATCATCGACGGG
GTGCCTCGACCCCTGCCTTCCTCCGCGCCCTCCGAGCTGGCGCGGGTGCA
GCATGAGACGGAGCTGGGGGGCGGACGGCCGCTGTCCATCTTTGCGGTGG
CGGGACCATTCACGACGAGCGACAGCTTGGTGTACGAGCCGCTGAACGAT
CTGCTCGGGGCGGTGAAGGTGGCGAAGCCGGACGTGGTGGTGCTCATGGG
GCCCTTTGTCGACGCGGAGCACCCCAAGGTGGGACGGGGGAGGGGAGAGG
AGGGGAGCGGGGCGGAGAAGGGCTCCCGTCGCGACAGCGGCGGCCAGCGG
GTCCACCTCGACCTCCGCGAGCTGCAGTCGTTCGCGCTCTTCCCGGGCCA
GGCGGTCGCAGTCCAGGGCGTGAACGGCTCGGGCGGCCGGATGGTGGCGC
GCGGGATCATCGACGGGGTGCCTCGACCCCTGCCTTCCTCCGCGCCCTCC
GAGCTGGCGCGGGTGCAGCATGAGACGGAGCTGGGGGGCGGACGGCCGCT
GTCCATCTTTGCGGTGGCGGGACCATTCACGACGAGCGACAGCTTGGTGT
ACGAGCCGCTGAACGATCTGCTCGGGGCGGTGAAGGTGGCGAAGCCGGAC
GTGGTGGTGCTCATGGGGCCCTTTGTCGACGCGGAGCACCCCAAGGTGGG
ACGGGGGAGGGGAGAGGAGGGGAGCGGGGCGGAG
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