mRNA_M-pyrifera_M_contig84833.19767.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig84833.19767.1
Unique NamemRNA_M-pyrifera_M_contig84833.19767.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig84833.19767.1 vs. uniprot
Match: A0A485KYE4_9STRA (Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial n=5 Tax=Aphanomyces TaxID=100860 RepID=A0A485KYE4_9STRA)

HSP 1 Score: 234 bits (597), Expect = 5.570e-73
Identity = 119/162 (73.46%), Postives = 142/162 (87.65%), Query Frame = 1
Query:    1 VAASEFDLNYIGLDGDIGCMVNGAGLAMATMDIIKLNGGEPANFLDVGGGATETQVAAAFRILNNDKNVRAILVNIFGGIMRCDVIAAGIVAAAKEIGLSKPIVLRLQGTNVTEAKEVIEHSGFRMILEDDLDTAAKKAVRVSDIVRQANEIAVDVKFTLPL 486
            V AS +DLNYIGLDG+IGC+VNGAGLAMATMDII+L GG PANFLDVGGGATE+QV  AF ILN D  V++ILVNIFGGIMRCDVIAAGI+AAAKE+G+ KPIV+RLQGTNV +A+ +IE+SGFRMI+ +DLD AA+KAV+++DIV QA E+ V+V F LPL
Sbjct:  276 VEASAYDLNYIGLDGNIGCLVNGAGLAMATMDIIQLFGGSPANFLDVGGGATESQVKKAFEILNADTQVKSILVNIFGGIMRCDVIAAGIIAAAKELGMKKPIVIRLQGTNVEKARALIENSGFRMIVANDLDDAAQKAVKIADIVSQAEEVHVNVSFELPL 437          
BLAST of mRNA_M-pyrifera_M_contig84833.19767.1 vs. uniprot
Match: A0A067CEF9_SAPPC (Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial n=4 Tax=Saprolegniaceae TaxID=4764 RepID=A0A067CEF9_SAPPC)

HSP 1 Score: 233 bits (595), Expect = 1.140e-72
Identity = 117/162 (72.22%), Postives = 142/162 (87.65%), Query Frame = 1
Query:    1 VAASEFDLNYIGLDGDIGCMVNGAGLAMATMDIIKLNGGEPANFLDVGGGATETQVAAAFRILNNDKNVRAILVNIFGGIMRCDVIAAGIVAAAKEIGLSKPIVLRLQGTNVTEAKEVIEHSGFRMILEDDLDTAAKKAVRVSDIVRQANEIAVDVKFTLPL 486
            V AS +DLNYIGLDG+IGC+VNGAGLAMATMDII L GG PANFLDVGGGATE+QV  AF ILN D  V++ILVNIFGGIMRCDVIAAG++AAAK++G+ KPIV+RLQGTNV +A+++IE+SGFRMI+ +DLD AA+KAV+++DIV QA E+ V+V F LPL
Sbjct:  277 VEASAYDLNYIGLDGNIGCLVNGAGLAMATMDIISLFGGSPANFLDVGGGATESQVKKAFEILNADPQVKSILVNIFGGIMRCDVIAAGVIAAAKDLGMKKPIVIRLQGTNVEKARQLIENSGFRMIVANDLDDAAQKAVKIADIVAQAEEVHVNVSFELPL 438          
BLAST of mRNA_M-pyrifera_M_contig84833.19767.1 vs. uniprot
Match: A0A024T9H2_9STRA (Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024T9H2_9STRA)

HSP 1 Score: 233 bits (594), Expect = 1.530e-72
Identity = 118/162 (72.84%), Postives = 141/162 (87.04%), Query Frame = 1
Query:    1 VAASEFDLNYIGLDGDIGCMVNGAGLAMATMDIIKLNGGEPANFLDVGGGATETQVAAAFRILNNDKNVRAILVNIFGGIMRCDVIAAGIVAAAKEIGLSKPIVLRLQGTNVTEAKEVIEHSGFRMILEDDLDTAAKKAVRVSDIVRQANEIAVDVKFTLPL 486
            V AS +DLNYIGLDG+IGC+VNGAGLAMATMDII+L GG PANFLDVGGGATE QV  AF ILN D  V++ILVNIFGGIMRCDVIAAGI+AAAK++G+ KPIV+RLQGTNV +A+ +IE+SGFRMI+ +DLD AA+KAV+++DIV QA E+ V+V F LPL
Sbjct:  275 VEASAYDLNYIGLDGNIGCLVNGAGLAMATMDIIQLFGGSPANFLDVGGGATEAQVKKAFEILNADNQVKSILVNIFGGIMRCDVIAAGIIAAAKDLGMKKPIVIRLQGTNVEKARALIENSGFRMIVANDLDDAAQKAVKIADIVTQAEEVHVNVSFELPL 436          
BLAST of mRNA_M-pyrifera_M_contig84833.19767.1 vs. uniprot
Match: A0A6S8FXT7_9STRA (Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial n=1 Tax=Aplanochytrium stocchinoi TaxID=215587 RepID=A0A6S8FXT7_9STRA)

HSP 1 Score: 232 bits (592), Expect = 5.010e-72
Identity = 116/162 (71.60%), Postives = 139/162 (85.80%), Query Frame = 1
Query:    1 VAASEFDLNYIGLDGDIGCMVNGAGLAMATMDIIKLNGGEPANFLDVGGGATETQVAAAFRILNNDKNVRAILVNIFGGIMRCDVIAAGIVAAAKEIGLSKPIVLRLQGTNVTEAKEVIEHSGFRMILEDDLDTAAKKAVRVSDIVRQANEIAVDVKFTLPL 486
            V A+++ LNYIGLDG IGC+VNGAGLAMATMDIIKLNGG PANFLDVGGGAT+ QV  AF ILN D+ V+AILVNIFGGIMRCDVIA+G+VAAA +IGL+KP+++RLQGTNV  AK+VIE SG+RMI+ DDLD AA KAVR+++IV+ A +I V V F +PL
Sbjct:  294 VEAAQYQLNYIGLDGSIGCLVNGAGLAMATMDIIKLNGGSPANFLDVGGGATKEQVQKAFEILNGDEKVKAILVNIFGGIMRCDVIASGVVAAAAKIGLAKPVIIRLQGTNVEAAKKVIEGSGYRMIMSDDLDDAASKAVRIAEIVKSAEDIQVGVSFEIPL 455          
BLAST of mRNA_M-pyrifera_M_contig84833.19767.1 vs. uniprot
Match: A0A2D4BIQ5_PYTIN (Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial n=3 Tax=Pythium TaxID=4797 RepID=A0A2D4BIQ5_PYTIN)

HSP 1 Score: 231 bits (590), Expect = 7.330e-72
Identity = 116/161 (72.05%), Postives = 140/161 (86.96%), Query Frame = 1
Query:    1 VAASEFDLNYIGLDGDIGCMVNGAGLAMATMDIIKLNGGEPANFLDVGGGATETQVAAAFRILNNDKNVRAILVNIFGGIMRCDVIAAGIVAAAKEIGLSKPIVLRLQGTNVTEAKEVIEHSGFRMILEDDLDTAAKKAVRVSDIVRQANEIAVDVKFTLP 483
            V AS++DLNYIGLDG+IGC+VNGAGLAMATMDII L GG PANFLDVGGGATE+QV  AF ILN D+ V++ILVNIFGGIMRCDVIAAGIVAAAK++GL KPI++RLQGTNV +A+E+I  SGF+MI+ DDL+ AA+KAV+++DIV QA E  ++V F LP
Sbjct:  282 VEASQYDLNYIGLDGNIGCLVNGAGLAMATMDIISLFGGSPANFLDVGGGATESQVKKAFEILNRDEQVKSILVNIFGGIMRCDVIAAGIVAAAKDLGLKKPIIIRLQGTNVEKARELIAQSGFKMIVADDLEDAAQKAVKIADIVAQAEEANINVSFELP 442          
BLAST of mRNA_M-pyrifera_M_contig84833.19767.1 vs. uniprot
Match: A0A5A8ENP4_CAFRO (Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial n=2 Tax=Sar TaxID=2698737 RepID=A0A5A8ENP4_CAFRO)

HSP 1 Score: 231 bits (588), Expect = 2.570e-71
Identity = 120/162 (74.07%), Postives = 138/162 (85.19%), Query Frame = 1
Query:    1 VAASEFDLNYIGLDGDIGCMVNGAGLAMATMDIIKLNGGEPANFLDVGGGATETQVAAAFRILNNDKNVRAILVNIFGGIMRCDVIAAGIVAAAKEIGLSKPIVLRLQGTNVTEAKEVIEHSGFRMILEDDLDTAAKKAVRVSDIVRQANEIAVDVKFTLPL 486
            V A++ DLNYIGLDG+IGCMVNGAGLAMATMDIIKL+GG PANFLDVGGGATE QV  A RIL+ D  V+ ILVNIFGGIMRCDVIA GI+AAAK++GL KP+V+RLQGTNV  AK VIE S  R+I+EDDLD AAKKAV +++IVRQAN+I +DV F LPL
Sbjct:  304 VEAAKHDLNYIGLDGNIGCMVNGAGLAMATMDIIKLHGGNPANFLDVGGGATEQQVKEALRILDADPGVQTILVNIFGGIMRCDVIAQGIIAAAKDVGLRKPLVVRLQGTNVEAAKRVIEASDVRIIMEDDLDEAAKKAVGLAEIVRQANQIKIDVHFELPL 465          
BLAST of mRNA_M-pyrifera_M_contig84833.19767.1 vs. uniprot
Match: K3WN63_GLOUD (Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WN63_GLOUD)

HSP 1 Score: 229 bits (585), Expect = 3.530e-71
Identity = 115/160 (71.88%), Postives = 140/160 (87.50%), Query Frame = 1
Query:    1 VAASEFDLNYIGLDGDIGCMVNGAGLAMATMDIIKLNGGEPANFLDVGGGATETQVAAAFRILNNDKNVRAILVNIFGGIMRCDVIAAGIVAAAKEIGLSKPIVLRLQGTNVTEAKEVIEHSGFRMILEDDLDTAAKKAVRVSDIVRQANEIAVDVKFTL 480
            V A+E+DLNYIGLDG+IGC+VNGAGLAMATMDII L GG PANFLDVGGGATE+QV  AF ILN D  V++ILVNIFGGIMRCDVIAAGIVAAAK++GL KPIV+RLQGTNV +A+E+I +SGF+MI+ DDLD AA+KAV+++DIV QA ++ ++V F +
Sbjct:  278 VEAAEYDLNYIGLDGNIGCLVNGAGLAMATMDIISLYGGSPANFLDVGGGATESQVKKAFEILNADNQVKSILVNIFGGIMRCDVIAAGIVAAAKDLGLKKPIVIRLQGTNVDKARELIANSGFKMIMTDDLDDAAEKAVKIADIVAQAEKVNINVAFEI 437          
BLAST of mRNA_M-pyrifera_M_contig84833.19767.1 vs. uniprot
Match: A0A482RUK3_9ARCH (ADP-forming succinate--CoA ligase subunit beta n=1 Tax=archaeon TaxID=1906665 RepID=A0A482RUK3_9ARCH)

HSP 1 Score: 225 bits (574), Expect = 1.420e-70
Identity = 115/158 (72.78%), Postives = 134/158 (84.81%), Query Frame = 1
Query:    1 VAASEFDLNYIGLDGDIGCMVNGAGLAMATMDIIKLNGGEPANFLDVGGGATETQVAAAFRILNNDKNVRAILVNIFGGIMRCDVIAAGIVAAAKEIGLSKPIVLRLQGTNVTEAKEVIEHSGFRMILEDDLDTAAKKAVRVSDIVRQANEIAVDVKF 474
            V A++ DLNYIGL G IGCMVNGAGLAMATMDIIKL GGEPANFLDVGGGA+E QV  AF +LN DK VR ILVNIFGGIMRCDVIA G++ AA  IGL KPI++RL+GTNV EAK++IE SG R+I+ DDL+ AAKKAV V+DIVRQA E+A++V+F
Sbjct:  184 VEAAKHDLNYIGLSGSIGCMVNGAGLAMATMDIIKLKGGEPANFLDVGGGASEQQVQKAFELLNADKQVRTILVNIFGGIMRCDVIAMGVINAAHNIGLKKPIIIRLKGTNVAEAKKLIEASGLRLIVTDDLEDAAKKAVHVADIVRQAEEVALNVQF 341          
BLAST of mRNA_M-pyrifera_M_contig84833.19767.1 vs. uniprot
Match: A0A5D6YJ52_9STRA (Succinate-CoA ligase subunit beta (Fragment) n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6YJ52_9STRA)

HSP 1 Score: 226 bits (576), Expect = 2.810e-70
Identity = 114/160 (71.25%), Postives = 137/160 (85.62%), Query Frame = 1
Query:    1 VAASEFDLNYIGLDGDIGCMVNGAGLAMATMDIIKLNGGEPANFLDVGGGATETQVAAAFRILNNDKNVRAILVNIFGGIMRCDVIAAGIVAAAKEIGLSKPIVLRLQGTNVTEAKEVIEHSGFRMILEDDLDTAAKKAVRVSDIVRQANEIAVDVKFTL 480
            V AS +DLNYIGLDG+IGC+VNGAGLAMATMDII L GG PANFLDVGGGATE QV  AF ILN D  V++ILVNIFGGIMRCDVIAAGI+AAA+++GL KPIV+RLQGTNV +A+E+I  SGF+MI+ DDLD AA+KAV+++DIV QA ++ ++V F L
Sbjct:  239 VEASAYDLNYIGLDGNIGCLVNGAGLAMATMDIISLYGGSPANFLDVGGGATEAQVKKAFEILNADDKVKSILVNIFGGIMRCDVIAAGIIAAARDLGLKKPIVIRLQGTNVEKARELIASSGFKMIMADDLDDAAEKAVKIADIVAQAEQVKLNVAFEL 398          
BLAST of mRNA_M-pyrifera_M_contig84833.19767.1 vs. uniprot
Match: A0A1Z5K8M0_FISSO (Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K8M0_FISSO)

HSP 1 Score: 226 bits (577), Expect = 6.700e-70
Identity = 119/158 (75.32%), Postives = 136/158 (86.08%), Query Frame = 1
Query:    1 VAASEFDLNYIGLDGDIGCMVNGAGLAMATMDIIKLNGGEPANFLDVGGGATETQVAAAFRILNNDKNVRAILVNIFGGIMRCDVIAAGIVAAAKEIGLSKPIVLRLQGTNVTEAKEVIEHSGFRMILEDDLDTAAKKAVRVSDIVRQANEIAVDVKF 474
            VAASE+DLNYIGLDG+IGCMVNGAGLAM+TMDII+L GG PANFLDVGGGA+E QV  AF ILN D  V+AILVNIFGGIMRCDVIA GI+ A KEIG+SKPIV+RLQGTNV +AK +IE S +RMIL DDL+ AA+KAV V++IV QA +I VDVKF
Sbjct:  281 VAASEYDLNYIGLDGNIGCMVNGAGLAMSTMDIIQLKGGSPANFLDVGGGASEQQVQKAFEILNADPKVKAILVNIFGGIMRCDVIANGILNAVKEIGISKPIVIRLQGTNVDKAKTLIEGSPYRMILADDLEAAAEKAVGVAEIVAQAEKIEVDVKF 438          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig84833.19767.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A485KYE4_9STRA5.570e-7373.46Succinate--CoA ligase [ADP-forming] subunit beta, ... [more]
A0A067CEF9_SAPPC1.140e-7272.22Succinate--CoA ligase [ADP-forming] subunit beta, ... [more]
A0A024T9H2_9STRA1.530e-7272.84Succinate--CoA ligase [ADP-forming] subunit beta, ... [more]
A0A6S8FXT7_9STRA5.010e-7271.60Succinate--CoA ligase [ADP-forming] subunit beta, ... [more]
A0A2D4BIQ5_PYTIN7.330e-7272.05Succinate--CoA ligase [ADP-forming] subunit beta, ... [more]
A0A5A8ENP4_CAFRO2.570e-7174.07Succinate--CoA ligase [ADP-forming] subunit beta, ... [more]
K3WN63_GLOUD3.530e-7171.88Succinate--CoA ligase [ADP-forming] subunit beta, ... [more]
A0A482RUK3_9ARCH1.420e-7072.78ADP-forming succinate--CoA ligase subunit beta n=1... [more]
A0A5D6YJ52_9STRA2.810e-7071.25Succinate-CoA ligase subunit beta (Fragment) n=1 T... [more]
A0A1Z5K8M0_FISSO6.700e-7075.32Succinate--CoA ligase [ADP-forming] subunit beta, ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig84833contigM-pyrifera_M_contig84833:3..491 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score235.0
Seed ortholog evalue2.6e-59
Seed eggNOG ortholog112098.XP_008618375.1
KEGG rclassRC00004,RC00014
KEGG koko:K01900
KEGG ReactionR00432,R00727
KEGG Pathwayko00020,ko00640,ko01100,ko01110,ko01120,ko01130,ko01200,map00020,map00640,map01100,map01110,map01120,map01130,map01200
KEGG ModuleM00009,M00011
Hectar predicted targeting categoryother localisation
EggNOG free text desc.succinate-CoA ligase (ADP-forming) activity
EggNOG OGsCOG0045@1,KOG2799@2759
EC6.2.1.4,6.2.1.5
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko04147
Exons1
Model size489
Cds size489
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816755.4855573-CDS-M-pyrifera_M_contig84833:2..4911622816755.4855573-CDS-M-pyrifera_M_contig84833:2..491Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84833 3..491 +
1692278159.263478-CDS-M-pyrifera_M_contig84833:2..4911692278159.263478-CDS-M-pyrifera_M_contig84833:2..491Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84833 3..491 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig84833.19767.1prot_M-pyrifera_M_contig84833.19767.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig84833 3..491 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig84833.19767.1

>prot_M-pyrifera_M_contig84833.19767.1 ID=prot_M-pyrifera_M_contig84833.19767.1|Name=mRNA_M-pyrifera_M_contig84833.19767.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=163bp
VAASEFDLNYIGLDGDIGCMVNGAGLAMATMDIIKLNGGEPANFLDVGGG
ATETQVAAAFRILNNDKNVRAILVNIFGGIMRCDVIAAGIVAAAKEIGLS
KPIVLRLQGTNVTEAKEVIEHSGFRMILEDDLDTAAKKAVRVSDIVRQAN
EIAVDVKFTLPL*
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mRNA from alignment at M-pyrifera_M_contig84833:3..491+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig84833.19767.1 ID=mRNA_M-pyrifera_M_contig84833.19767.1|Name=mRNA_M-pyrifera_M_contig84833.19767.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=489bp|location=Sequence derived from alignment at M-pyrifera_M_contig84833:3..491+ (Macrocystis pyrifera P11B4 male)
GTTGCGGCGAGCGAGTTTGACTTGAACTACATTGGTCTGGATGGAGACAT TGGGTGCATGGTGAACGGTGCGGGATTGGCAATGGCCACGATGGACATTA TCAAGCTCAACGGAGGGGAGCCAGCAAACTTTCTCGACGTGGGAGGAGGG GCGACCGAGACACAGGTTGCCGCAGCCTTCCGTATCCTCAACAATGACAA GAATGTCAGAGCCATCCTCGTCAACATCTTTGGAGGCATCATGCGATGCG ACGTCATTGCGGCAGGTATCGTTGCGGCTGCCAAGGAAATCGGACTCTCC AAACCAATCGTGCTCCGCCTCCAAGGCACCAACGTCACGGAAGCAAAGGA AGTCATCGAACACTCGGGGTTCAGAATGATCCTCGAAGATGACCTCGACA CCGCAGCCAAAAAAGCCGTCAGGGTCTCTGACATCGTCAGACAAGCCAAC GAAATCGCAGTCGACGTCAAATTCACGCTCCCTCTGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig84833:3..491+

>mRNA_M-pyrifera_M_contig84833.19767.1 ID=mRNA_M-pyrifera_M_contig84833.19767.1|Name=mRNA_M-pyrifera_M_contig84833.19767.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=978bp|location=Sequence derived from alignment at M-pyrifera_M_contig84833:3..491+ (Macrocystis pyrifera P11B4 male)
GTTGCGGCGAGCGAGTTTGACTTGAACTACATTGGTCTGGATGGAGACAT
TGGGTGCATGGTGAACGGTGCGGGATTGGCAATGGCCACGATGGACATTA
TCAAGCTCAACGGAGGGGAGCCAGCAAACTTTCTCGACGTGGGAGGAGGG
GCGACCGAGACACAGGTTGCCGCAGCCTTCCGTATCCTCAACAATGACAA
GAATGTCAGAGCCATCCTCGTCAACATCTTTGGAGGCATCATGCGATGCG
ACGTCATTGCGGCAGGTATCGTTGCGGCTGCCAAGGAAATCGGACTCTCC
AAACCAATCGTGCTCCGCCTCCAAGGCACCAACGTCACGGAAGCAAAGGA
AGTCATCGAACACTCGGGGTTCAGAATGATCCTCGAAGATGACCTCGACA
CCGCAGCCAAAAAAGCCGTCAGGGTCTCTGACATCGTCAGACAAGCCAAC
GAAATCGCAGTCGACGTCAAATTCACGCTCCCTCTGTAAGTTGCGGCGAG
CGAGTTTGACTTGAACTACATTGGTCTGGATGGAGACATTGGGTGCATGG
TGAACGGTGCGGGATTGGCAATGGCCACGATGGACATTATCAAGCTCAAC
GGAGGGGAGCCAGCAAACTTTCTCGACGTGGGAGGAGGGGCGACCGAGAC
ACAGGTTGCCGCAGCCTTCCGTATCCTCAACAATGACAAGAATGTCAGAG
CCATCCTCGTCAACATCTTTGGAGGCATCATGCGATGCGACGTCATTGCG
GCAGGTATCGTTGCGGCTGCCAAGGAAATCGGACTCTCCAAACCAATCGT
GCTCCGCCTCCAAGGCACCAACGTCACGGAAGCAAAGGAAGTCATCGAAC
ACTCGGGGTTCAGAATGATCCTCGAAGATGACCTCGACACCGCAGCCAAA
AAAGCCGTCAGGGTCTCTGACATCGTCAGACAAGCCAACGAAATCGCAGT
CGACGTCAAATTCACGCTCCCTCTGTAA
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