mRNA_M-pyrifera_M_contig84766.19750.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig84766.19750.1
Unique NamemRNA_M-pyrifera_M_contig84766.19750.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig84766.19750.1 vs. uniprot
Match: A0A7S4HZU8_9EUKA (Palmitoyltransferase n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4HZU8_9EUKA)

HSP 1 Score: 122 bits (306), Expect = 9.240e-30
Identity = 79/248 (31.85%), Postives = 114/248 (45.97%), Query Frame = 1
Query:   40 LPLAVDVLNTFFPIGRIVSQYTSRVLISMLVGLYSAALYFFFALVIPFSLET-----------VADGGEW---------WTPGSTAAVVAGSLFCVWLWINTVYCYYQAIMQSPSKLVAASTAPKNAAPEGAAVQGLRKRDTDRAVEIGTCAPTSAKVPRSATTHHCRQCGHCIPHMDHHCPFTANCVSTGPKGNFVFFFLFIFYSTVGCLFACATNALPFYHCFLLGEETVACSYVGTMSIILIPGI 723
            +PL +D +NTF PIGR+V  Y SR LI +L   Y A++Y++F +VIP+  E+           +    EW         W+      VV G+   +WLW+NT++ Y+Q +  SP  L                        T+  ++         K+ +   THH                      ++G  GNFVFFFLFIFY+  GC  A +T  LPFY+C+L  E +  C  VG MS+I IPG+
Sbjct:    1 MPLFIDTINTFIPIGRLVHVYLSRFLIIVLAIQYVASIYYYFTMVIPYYSESATSLDVNAYRSLVSSYEWTDTYQNWESWSFSHQFWVVFGACIALWLWMNTIFSYFQTVRYSPLPLPPVEL-------------------TEEEIKQNRNYCQKCKIIKKPATHHXXXXXXXXXXXXXXXXXXXXXXTSGKTGNFVFFFLFIFYTAAGCFLAVSTMTLPFYNCYLQRELSPRCVAVGNMSLIYIPGL 229          
BLAST of mRNA_M-pyrifera_M_contig84766.19750.1 vs. uniprot
Match: A0A7S0D909_9EUKA (Palmitoyltransferase n=2 Tax=Amorphochlora amoebiformis TaxID=1561963 RepID=A0A7S0D909_9EUKA)

HSP 1 Score: 99.0 bits (245), Expect = 2.660e-20
Identity = 84/290 (28.97%), Postives = 123/290 (42.41%), Query Frame = 1
Query:   25 VGVSMLPLAVDVLNTFFP---IGRIVSQYTSRVLISMLVGLYSAALYFFFALVIPFSL--------ETVADGGEWWTP-------------------GSTAAVVAGSLFCVWLWINTVYCYYQAIMQS------PSKLVAASTAPKNAAPEGAAVQGL-----------------RKRDTDRAVEIGTCAPTSAKVPRSATTHHCRQCGHCIPHMDHHCPFTANCVSTGPKGNFVFFFLFIFYSTVGCLFACATNALPFYHCFLLGEETVA------CSYVGTMSIILIP 717
            VG+SMLPLA+DV+N  FP   I R  S     VL   +  +  A L FFF   +P++L        +         TP                    S+    A ++F +W WIN VY + +A++        P     A+   K   P G     +                 RK  T R    G C       P+     HCR C  C+  +DHHCPFT NCV +G   NF+FFFLF+ Y ++G L++   ++  FY C +L   +        CS++GT +++ +P
Sbjct:   11 VGISMLPLAIDVVNAAFPNYKIARATSTVVQPVLAFFVFFIAVACLNFFFFSYLPYTLFLDDKLFHDRAPPTAYCTTPQEGCDDRAISLADLYSKAYSSSHRAYAHTVFALWAWINVVYNFTKAVLTDAGTRDHPEVSKFANPGEKAEMPRGKPRDRVVRKASGTKEEKAKNSEGRKSKTTRVCPSGVCNGR----PKVPGFRHCRVCRVCVALLDHHCPFTGNCVGSG---NFLFFFLFLAYCSLGLLYSLVVSSRAFYTCVILPPASPMSLDRQWCSFLGTNALLALP 293          
BLAST of mRNA_M-pyrifera_M_contig84766.19750.1 vs. uniprot
Match: UPI000644AA7B (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644AA7B)

HSP 1 Score: 78.2 bits (191), Expect = 2.430e-13
Identity = 66/247 (26.72%), Postives = 100/247 (40.49%), Query Frame = 1
Query:   25 VGVSMLPLAVDVLNTFFP---IGRIVSQYTSRVLISMLVGLYSAALYFFFALVIPFSLETVADGGEWWTPGSTAAVVAGSLFCVWLWINTVYCYYQAIMQSPSKLVAASTAPKNAAPEGAAVQGLRKRDTDRAVEIGTC----------------APTSAKVPRSATTHHCRQCGHCIPHMDHHCPFTANCVSTGPKGNFVFFFLFIFYSTVGCLFACATNALPFYHCFLLGEETVACSYVGTMSII 708
            V V +LPL  D+LN  FP   IGRI  +    VL++ +  L   ALY +++   P+ L+T       +    T+          ++ I  V  YY   + + S +                   ++ +D D  V   T                     A       ++HCR C  C  HMDHHCPF ANCV T    +FV    F+ Y+T+G  +A   + LP+  C L+   +  C ++   S I
Sbjct:   12 VVVILLPLLTDLLNGLFPNARIGRIAQKLVGHVLVTFITALILVALYLWYSTYFPYFLDT-------YRLDHTSPHFHLHCIITFILIANVLIYYLYAINTSSYIKQ---------------HPIQSQDDDSVVATTTTHTXXXXXXXXXXXXXXXXXGAMKRSEQKSYHCRICKRCTHHMDHHCPFIANCVGTRNHCHFV---NFLMYTTLGVFYAMYLSWLPYRDCILMSSTSATCQHLSKHSYI 233          
BLAST of mRNA_M-pyrifera_M_contig84766.19750.1 vs. uniprot
Match: A0A7S2KHR6_BIGNA (Palmitoyltransferase (Fragment) n=1 Tax=Bigelowiella natans TaxID=227086 RepID=A0A7S2KHR6_BIGNA)

HSP 1 Score: 70.9 bits (172), Expect = 3.340e-12
Identity = 32/74 (43.24%), Postives = 46/74 (62.16%), Query Frame = 1
Query:  439 TSAKVPRSATTHHCRQCGHCIPHMDHHCPFTANCVSTGPKGNFVFFFLFIFYSTVGCLFACATNALPFYHCFLL 660
            +SA +P      HC+ CG C  +MDHHCPFT NCV  G   NF++F+LF+ Y ++G L+A   ++  F  C +L
Sbjct:   31 SSAMMPPRLFFRHCKVCGTCTAYMDHHCPFTGNCVGAG---NFIYFYLFLGYCSLGLLYALVLSSRAFLRCIIL 101          
BLAST of mRNA_M-pyrifera_M_contig84766.19750.1 vs. uniprot
Match: A0A7S2TRY0_9EUKA (Palmitoyltransferase (Fragment) n=1 Tax=Lotharella oceanica TaxID=641309 RepID=A0A7S2TRY0_9EUKA)

HSP 1 Score: 71.6 bits (174), Expect = 6.130e-12
Identity = 42/137 (30.66%), Postives = 67/137 (48.91%), Query Frame = 1
Query:  247 AGSLFCVWLWINTVYCYYQAIMQSP-SKLVAASTAPKNAAPEGAAVQGLRKRDTDRAVEIGTCAPTSAKVPRSATT-HHCRQCGHCIPHMDHHCPFTANCVSTGPKGNFVFFFLFIFYSTVGCLFACATNALPFYHC 651
            A +L  +W+W+  +Y +  A++ +P ++L  AS +     P   A         D          + A + R      HCR CG C+  MDHHCPFT+NCV      NF+FF+LF+ Y ++G  ++   ++  F  C
Sbjct:   23 AHTLVAMWMWVGVMYNFSAAVLTNPGTRLHLASLSYLRRHPRATACAPCESSSRDSVSLQKLSEKSRALLMRKPPGFRHCRVCGTCVGWMDHHCPFTSNCVGAN---NFIFFYLFLAYCSLGLFYSLVVSSRAFLTC 156          
BLAST of mRNA_M-pyrifera_M_contig84766.19750.1 vs. uniprot
Match: A0A067CYI4_SAPPC (Palmitoyltransferase n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067CYI4_SAPPC)

HSP 1 Score: 64.3 bits (155), Expect = 1.010e-8
Identity = 42/111 (37.84%), Postives = 53/111 (47.75%), Query Frame = 1
Query:  286 VYCYYQAIMQSPSKLVAASTAPKNAAPEGAAVQGLRKRDTDRAVEIGTCAPTSAKVPRSATTHHCRQCGHCIPHMDHHCPFTANCVSTGPKGNFVFFFLFIFYSTVGCLFA 618
            V  Y  AI  SPS  VAA  +P+               D D   E   C       P S   HHC  C  CI HMDHHCP+T+NCV    K  F+   LF+FY+++ CL++
Sbjct:   42 VVSYAGAIFISPSLSVAALLSPE---------------DGDADTEARYCEKCDCVKPESF--HHCSVCMRCISHMDHHCPWTSNCVGERTKKIFI---LFLFYTSLSCLWS 132          
BLAST of mRNA_M-pyrifera_M_contig84766.19750.1 vs. uniprot
Match: A0A1V9Z8Q8_9STRA (Palmitoyltransferase n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9Z8Q8_9STRA)

HSP 1 Score: 62.8 bits (151), Expect = 2.360e-8
Identity = 26/49 (53.06%), Postives = 35/49 (71.43%), Query Frame = 1
Query:  472 HHCRQCGHCIPHMDHHCPFTANCVSTGPKGNFVFFFLFIFYSTVGCLFA 618
            HHC  CG CI HMDHHCP+T+NCV    K  F+   LF+FY+++ CL++
Sbjct:   59 HHCSVCGRCISHMDHHCPWTSNCVGLRTKKVFI---LFLFYTSISCLWS 104          
BLAST of mRNA_M-pyrifera_M_contig84766.19750.1 vs. uniprot
Match: A0A818LTE1_9BILA (Palmitoyltransferase (Fragment) n=2 Tax=Rotaria sp. Silwood2 TaxID=2762512 RepID=A0A818LTE1_9BILA)

HSP 1 Score: 62.0 bits (149), Expect = 6.210e-8
Identity = 28/59 (47.46%), Postives = 35/59 (59.32%), Query Frame = 1
Query:  415 VEIGTCAPTSAKVPRSATTHHCRQCGHCIPHMDHHCPFTANCVSTGPKGNFVFFFLFIF 591
            V+      TS  + +   THHCR CGHCI  MDHHCP+  NCV    + N+ FF LF+F
Sbjct:   51 VDFDVAQCTSCFIVKPPRTHHCRNCGHCIDTMDHHCPWLCNCVG---RRNYRFFLLFLF 106          
BLAST of mRNA_M-pyrifera_M_contig84766.19750.1 vs. uniprot
Match: A0A197K4U3_9FUNG (Palmitoyltransferase n=1 Tax=Linnemannia elongata AG-77 TaxID=1314771 RepID=A0A197K4U3_9FUNG)

HSP 1 Score: 61.2 bits (147), Expect = 2.850e-7
Identity = 28/58 (48.28%), Postives = 38/58 (65.52%), Query Frame = 1
Query:  472 HHCRQCGHCIPHMDHHCPFTANCVSTGPKGNFVFFFLFIFYSTVGCLFACATNALPFY 645
            HHCR C  C+  MDHHCP+  NCV    + N+ FF LF+FY++V C+F  A + +P Y
Sbjct:  175 HHCRVCKRCVLKMDHHCPWVLNCVG---QDNYKFFVLFVFYTSVHCIFILA-SLIPLY 228          
BLAST of mRNA_M-pyrifera_M_contig84766.19750.1 vs. uniprot
Match: B8MQF4_TALSN (Palmitoyltransferase PFA4 n=1 Tax=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) TaxID=441959 RepID=B8MQF4_TALSN)

HSP 1 Score: 61.2 bits (147), Expect = 3.030e-7
Identity = 39/108 (36.11%), Postives = 53/108 (49.07%), Query Frame = 1
Query:  262 CVWLWINTVYCYYQAIMQSPSKLVAASTAPKNAAPEGAAVQGLRKRDTDRAVEIGTCAPTSAKVPRSATTHHCRQCGHCIPHMDHHCPFTANCVSTGPKGNFVFFFLF 585
            C+W+      CYY+A    P ++      P    P+G   Q +   D       G CA  + K PR+   HHC+ C  CIP MDHHCP+T NCVS     +FV F ++
Sbjct:   53 CIWI------CYYRACTVDPGRV------PNEWTPKG---QNISLDDNGDYRNDGKCA--AFKPPRA---HHCKICQRCIPKMDHHCPWTRNCVSHFTLPHFVRFLVY 140          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig84766.19750.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S4HZU8_9EUKA9.240e-3031.85Palmitoyltransferase n=1 Tax=Vannella sp. CB-2014 ... [more]
A0A7S0D909_9EUKA2.660e-2028.97Palmitoyltransferase n=2 Tax=Amorphochlora amoebif... [more]
UPI000644AA7B2.430e-1326.72hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A7S2KHR6_BIGNA3.340e-1243.24Palmitoyltransferase (Fragment) n=1 Tax=Bigelowiel... [more]
A0A7S2TRY0_9EUKA6.130e-1230.66Palmitoyltransferase (Fragment) n=1 Tax=Lotharella... [more]
A0A067CYI4_SAPPC1.010e-837.84Palmitoyltransferase n=2 Tax=Saprolegnia TaxID=476... [more]
A0A1V9Z8Q8_9STRA2.360e-853.06Palmitoyltransferase n=1 Tax=Thraustotheca clavata... [more]
A0A818LTE1_9BILA6.210e-847.46Palmitoyltransferase (Fragment) n=2 Tax=Rotaria sp... [more]
A0A197K4U3_9FUNG2.850e-748.28Palmitoyltransferase n=1 Tax=Linnemannia elongata ... [more]
B8MQF4_TALSN3.030e-736.11Palmitoyltransferase PFA4 n=1 Tax=Talaromyces stip... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig84766contigM-pyrifera_M_contig84766:94..870 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score106.3
Seed ortholog evalue2e-20
Seed eggNOG ortholog227086.JGI_V11_82292
KEGG koko:K18272,ko:K18932,ko:K20028
Hectar predicted targeting categorysignal anchor
EggNOG free text desc.protein-cysteine S-palmitoyltransferase activity
EggNOG OGsCOG5273@1,KOG1315@2759
EC2.3.1.225
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko04131,ko04147
Exons2
Model size726
Cds size717
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig84766.19750.1prot_M-pyrifera_M_contig84766.19750.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig84766 103..870 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816754.4933264-UTR-M-pyrifera_M_contig84766:93..1021622816754.4933264-UTR-M-pyrifera_M_contig84766:93..102Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig84766 94..102 +
1692278158.6159742-UTR-M-pyrifera_M_contig84766:93..1021692278158.6159742-UTR-M-pyrifera_M_contig84766:93..102Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig84766 94..102 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816754.5067284-CDS-M-pyrifera_M_contig84766:102..5421622816754.5067284-CDS-M-pyrifera_M_contig84766:102..542Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84766 103..542 +
1692278158.6275206-CDS-M-pyrifera_M_contig84766:102..5421692278158.6275206-CDS-M-pyrifera_M_contig84766:102..542Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84766 103..542 +
1622816754.5191188-CDS-M-pyrifera_M_contig84766:593..8701622816754.5191188-CDS-M-pyrifera_M_contig84766:593..870Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84766 594..870 +
1692278158.6375651-CDS-M-pyrifera_M_contig84766:593..8701692278158.6375651-CDS-M-pyrifera_M_contig84766:593..870Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84766 594..870 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig84766.19750.1

>prot_M-pyrifera_M_contig84766.19750.1 ID=prot_M-pyrifera_M_contig84766.19750.1|Name=mRNA_M-pyrifera_M_contig84766.19750.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=239bp
MLIVMVGVSMLPLAVDVLNTFFPIGRIVSQYTSRVLISMLVGLYSAALYF
FFALVIPFSLETVADGGEWWTPGSTAAVVAGSLFCVWLWINTVYCYYQAI
MQSPSKLVAASTAPKNAAPEGAAVQGLRKRDTDRAVEIGTCAPTSAKVPR
SATTHHCRQCGHCIPHMDHHCPFTANCVSTGPKGNFVFFFLFIFYSTVGC
LFACATNALPFYHCFLLGEETVACSYVGTMSIILIPGIV
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mRNA from alignment at M-pyrifera_M_contig84766:94..870+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig84766.19750.1 ID=mRNA_M-pyrifera_M_contig84766.19750.1|Name=mRNA_M-pyrifera_M_contig84766.19750.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=777bp|location=Sequence derived from alignment at M-pyrifera_M_contig84766:94..870+ (Macrocystis pyrifera P11B4 male)
GTGGTGGACATGCTGATTGTGATGGTTGGCGTGAGCATGCTGCCCCTTGC AGTCGACGTGCTAAACACCTTTTTCCCCATTGGCCGCATTGTCAGCCAGT ACACGAGCAGAGTGCTGATCTCCATGTTGGTTGGATTGTACAGTGCAGCC CTGTACTTCTTCTTTGCCCTCGTCATCCCGTTCTCATTGGAGACAGTGGC AGATGGTGGAGAGTGGTGGACGCCTGGCAGCACAGCAGCTGTTGTGGCAG GGTCGTTGTTTTGTGTGTGGCTTTGGATCAACACAGTGTATTGCTACTAT CAGGCCATAATGCAGTCACCCTCTAAGCTCGTGGCAGCCAGTACGGCACC CAAGAATGCTGCGCCAGAGGGTGCAGCAGTCCAGGGCTTGAGGAAGAGGG ATACAGACAGGGCTGTAGAGATCGGTACATGCGCTCCCACCAGTGCGAAG TCACCTTCGGATGAAGGGGTGAAGAGGATCCGGGTGTGCAGGATATGCAG GGTGCCCAGGTCTGCCACTACCCACCACTGCCGACAGTGCGGCCACTGCA TTCCACACATGGATCACCACTGCCCATTCACAGCAAACTGCGTCTCCACT GGACCAAAGGGGAACTTCGTCTTCTTCTTTCTCTTCATCTTCTACTCCAC CGTGGGCTGCCTCTTTGCGTGCGCCACCAACGCTTTACCATTCTACCATT GCTTTCTGCTGGGAGAGGAAACGGTTGCTTGCTCCTATGTGGGCACCATG AGCATCATTCTCATCCCAGGCATCGTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig84766:94..870+

>mRNA_M-pyrifera_M_contig84766.19750.1 ID=mRNA_M-pyrifera_M_contig84766.19750.1|Name=mRNA_M-pyrifera_M_contig84766.19750.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1434bp|location=Sequence derived from alignment at M-pyrifera_M_contig84766:94..870+ (Macrocystis pyrifera P11B4 male)
ATGCTGATTGTGATGGTTGGCGTGAGCATGCTGCCCCTTGCAGTCGACGT
GCTAAACACCTTTTTCCCCATTGGCCGCATTGTCAGCCAGTACACGAGCA
GAGTGCTGATCTCCATGTTGGTTGGATTGTACAGTGCAGCCCTGTACTTC
TTCTTTGCCCTCGTCATCCCGTTCTCATTGGAGACAGTGGCAGATGGTGG
AGAGTGGTGGACGCCTGGCAGCACAGCAGCTGTTGTGGCAGGGTCGTTGT
TTTGTGTGTGGCTTTGGATCAACACAGTGTATTGCTACTATCAGGCCATA
ATGCAGTCACCCTCTAAGCTCGTGGCAGCCAGTACGGCACCCAAGAATGC
TGCGCCAGAGGGTGCAGCAGTCCAGGGCTTGAGGAAGAGGGATACAGACA
GGGCTGTAGAGATCGGTACATGCGCTCCCACCAGTGCGAAATGCTGATTG
TGATGGTTGGCGTGAGCATGCTGCCCCTTGCAGTCGACGTGCTAAACACC
TTTTTCCCCATTGGCCGCATTGTCAGCCAGTACACGAGCAGAGTGCTGAT
CTCCATGTTGGTTGGATTGTACAGTGCAGCCCTGTACTTCTTCTTTGCCC
TCGTCATCCCGTTCTCATTGGAGACAGTGGCAGATGGTGGAGAGTGGTGG
ACGCCTGGCAGCACAGCAGCTGTTGTGGCAGGGTCGTTGTTTTGTGTGTG
GCTTTGGATCAACACAGTGTATTGCTACTATCAGGCCATAATGCAGTCAC
CCTCTAAGCTCGTGGCAGCCAGTACGGCACCCAAGAATGCTGCGCCAGAG
GGTGCAGCAGTCCAGGGCTTGAGGAAGAGGGATACAGACAGGGCTGTAGA
GATCGGTACATGCGCTCCCACCAGTGCGAAGGTGCCCAGGTCTGCCACTA
CCCACCACTGCCGACAGTGCGGCCACTGCATTCCACACATGGATCACCAC
TGCCCATTCACAGCAAACTGCGTCTCCACTGGACCAAAGGGGAACTTCGT
CTTCTTCTTTCTCTTCATCTTCTACTCCACCGTGGGCTGCCTCTTTGCGT
GCGCCACCAACGCTTTACCATTCTACCATTGCTTTCTGCTGGGAGAGGAA
ACGGTTGCTTGCTCCTATGTGGGCACCATGAGCATCATTCTCATCCCAGG
CATCGTCGGTGCCCAGGTCTGCCACTACCCACCACTGCCGACAGTGCGGC
CACTGCATTCCACACATGGATCACCACTGCCCATTCACAGCAAACTGCGT
CTCCACTGGACCAAAGGGGAACTTCGTCTTCTTCTTTCTCTTCATCTTCT
ACTCCACCGTGGGCTGCCTCTTTGCGTGCGCCACCAACGCTTTACCATTC
TACCATTGCTTTCTGCTGGGAGAGGAAACGGTTGCTTGCTCCTATGTGGG
CACCATGAGCATCATTCTCATCCCAGGCATCGTC
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