mRNA_M-pyrifera_M_contig83744.19550.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig83744.19550.1
Unique NamemRNA_M-pyrifera_M_contig83744.19550.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig83744.19550.1 vs. uniprot
Match: A0A0G4FJW1_9ALVE (Methylenetetrahydrofolate reductase n=1 Tax=Chromera velia CCMP2878 TaxID=1169474 RepID=A0A0G4FJW1_9ALVE)

HSP 1 Score: 122 bits (305), Expect = 1.670e-30
Identity = 53/80 (66.25%), Postives = 64/80 (80.00%), Query Frame = 1
Query:    4 AVTWGVFPNKEILQPTVMDSNALPIWKDEAFSLWQSEWGALYEPDSKSRELIDDIHDNWWLVDLVDNDFVNGDIFKPFFE 243
            AVTWGVFPN EI QPTV+D  +  IWKDEAFSLW  EWG LY  DSKSR++I++I +NWWLV++VDNDFV+GD+F    E
Sbjct:  513 AVTWGVFPNSEIKQPTVVDYASFVIWKDEAFSLWVDEWGCLYPDDSKSRQIIEEIANNWWLVNIVDNDFVSGDLFNHLTE 592          
BLAST of mRNA_M-pyrifera_M_contig83744.19550.1 vs. uniprot
Match: F4PLP1_CAVFA (Methylenetetrahydrofolate reductase n=1 Tax=Cavenderia fasciculata (strain SH3) TaxID=1054147 RepID=F4PLP1_CAVFA)

HSP 1 Score: 118 bits (295), Expect = 3.850e-29
Identity = 46/82 (56.10%), Postives = 72/82 (87.80%), Query Frame = 1
Query:    4 AVTWGVFPNKEILQPTVMDSNALPIWKDEAFSLWQSEWGALYEPDSKSRELIDDIHDNWWLVDLVDNDFVNGDIFKPFFELL 249
            AVTWG+FP KE++QPTV+D ++  +WKDEAF+LWQS W +LY+ +S SR+++D++ DN++L+++VDN+FVNG+IF+PF +L+
Sbjct:  506 AVTWGIFPGKEVVQPTVVDHSSFLVWKDEAFALWQSRWSSLYDENSTSRKVLDEMIDNYYLINVVDNNFVNGNIFQPFIDLV 587          
BLAST of mRNA_M-pyrifera_M_contig83744.19550.1 vs. uniprot
Match: A0A0D2KCH0_9CHLO (Methylenetetrahydrofolate reductase n=1 Tax=Monoraphidium neglectum TaxID=145388 RepID=A0A0D2KCH0_9CHLO)

HSP 1 Score: 118 bits (295), Expect = 3.890e-29
Identity = 50/76 (65.79%), Postives = 59/76 (77.63%), Query Frame = 1
Query:    4 AVTWGVFPNKEILQPTVMDSNALPIWKDEAFSLWQSEWGALYEPDSKSRELIDDIHDNWWLVDLVDNDFVNGDIFK 231
            AVTWGVFP  E+ QPTV+D  +  +WKDEAF LW SEWGALYE  S SR+L+  IHD WWLV +VDND+V GD+FK
Sbjct:  519 AVTWGVFPGSEVEQPTVVDPVSFEVWKDEAFGLWLSEWGALYEEASPSRQLLQTIHDTWWLVSVVDNDYVTGDLFK 594          
BLAST of mRNA_M-pyrifera_M_contig83744.19550.1 vs. uniprot
Match: A0A357HDD7_9BACT (Uncharacterized protein n=1 Tax=Candidatus Marinimicrobia bacterium TaxID=2026760 RepID=A0A357HDD7_9BACT)

HSP 1 Score: 110 bits (274), Expect = 7.270e-29
Identity = 46/76 (60.53%), Postives = 65/76 (85.53%), Query Frame = 1
Query:    4 AVTWGVFPNKEILQPTVMDSNALPIWKDEAFSLWQSEWGALYEPDSKSRELIDDIHDNWWLVDLVDNDFVNGDIFK 231
            AVTWGVFP+KEI+QPT++D+ +  IWKDEAFSLW ++W ++YE  S+S ELI +I++ ++LV++VDNDFV+GDI K
Sbjct:   81 AVTWGVFPDKEIMQPTIVDTRSFLIWKDEAFSLWMNDWASIYEAKSESYELIKEIYNTYYLVNVVDNDFVDGDILK 156          
BLAST of mRNA_M-pyrifera_M_contig83744.19550.1 vs. uniprot
Match: A0A2D4BX93_PYTIN (Methylenetetrahydrofolate reductase (NAD(P)H) n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BX93_PYTIN)

HSP 1 Score: 117 bits (292), Expect = 1.280e-28
Identity = 48/79 (60.76%), Postives = 66/79 (83.54%), Query Frame = 1
Query:    1 IAVTWGVFPNKEILQPTVMDSNALPIWKDEAFSLWQSEWGALYEPDSKSRELIDDIHDNWWLVDLVDNDFVNGDIFKPF 237
            IAVTWGVFPNKE+LQPT++DSN+   WKDEAFSLW  EW ++Y+ DS++ E+I +IHD ++LV +VDNDF+ G+I++ F
Sbjct:  478 IAVTWGVFPNKEVLQPTIVDSNSFLAWKDEAFSLWLKEWASIYDDDSRAAEIIHEIHDTYFLVSIVDNDFIRGNIWEIF 556          
BLAST of mRNA_M-pyrifera_M_contig83744.19550.1 vs. uniprot
Match: A0A2V0P1W0_9CHLO (Methylenetetrahydrofolate reductase n=1 Tax=Raphidocelis subcapitata TaxID=307507 RepID=A0A2V0P1W0_9CHLO)

HSP 1 Score: 116 bits (290), Expect = 1.740e-28
Identity = 49/76 (64.47%), Postives = 60/76 (78.95%), Query Frame = 1
Query:    4 AVTWGVFPNKEILQPTVMDSNALPIWKDEAFSLWQSEWGALYEPDSKSRELIDDIHDNWWLVDLVDNDFVNGDIFK 231
            AVTWGVFP KE+ QPTV+D  +  +WKDEAF LW +EWGALYE  S SR+L+  IHD WWLV +VDND+V G++FK
Sbjct:  494 AVTWGVFPGKEVEQPTVVDPVSFGVWKDEAFGLWLTEWGALYEEGSPSRKLLQRIHDTWWLVSVVDNDYVGGNLFK 569          
BLAST of mRNA_M-pyrifera_M_contig83744.19550.1 vs. uniprot
Match: F0ZME6_DICPU (Methylenetetrahydrofolate reductase n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0ZME6_DICPU)

HSP 1 Score: 115 bits (288), Expect = 3.670e-28
Identity = 46/82 (56.10%), Postives = 69/82 (84.15%), Query Frame = 1
Query:    4 AVTWGVFPNKEILQPTVMDSNALPIWKDEAFSLWQSEWGALYEPDSKSRELIDDIHDNWWLVDLVDNDFVNGDIFKPFFELL 249
            AVTWG+FP KEI+QPTV+D N+  +WKDEAF+LW+++W   YE DSKSRE++D + D+++L ++VDN+F++G+IF PF +L+
Sbjct:  509 AVTWGIFPGKEIIQPTVVDHNSFMVWKDEAFALWETQWANNYEKDSKSREILDKMIDSYYLFNIVDNNFISGNIFSPFSDLV 590          
BLAST of mRNA_M-pyrifera_M_contig83744.19550.1 vs. uniprot
Match: A0A662WW66_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662WW66_9STRA)

HSP 1 Score: 111 bits (278), Expect = 4.470e-28
Identity = 48/79 (60.76%), Postives = 64/79 (81.01%), Query Frame = 1
Query:    1 IAVTWGVFPNKEILQPTVMDSNALPIWKDEAFSLWQSEWGALYEPDSKSRELIDDIHDNWWLVDLVDNDFVNGDIFKPF 237
            +AVTWGVFPNKEILQPT++DS++  +WKDEAF+LW   W +LYE  S+S  L+ +IHD+++LV +VDNDFVNG I+  F
Sbjct:  207 VAVTWGVFPNKEILQPTIVDSSSFLVWKDEAFALWLKLWASLYEEASQSARLLREIHDSYFLVSIVDNDFVNGSIWDLF 285          
BLAST of mRNA_M-pyrifera_M_contig83744.19550.1 vs. uniprot
Match: A0A2D5Q091_9BACT (Methylenetetrahydrofolate reductase (Fragment) n=1 Tax=Candidatus Marinimicrobia bacterium TaxID=2026760 RepID=A0A2D5Q091_9BACT)

HSP 1 Score: 112 bits (279), Expect = 5.210e-28
Identity = 47/76 (61.84%), Postives = 65/76 (85.53%), Query Frame = 1
Query:    4 AVTWGVFPNKEILQPTVMDSNALPIWKDEAFSLWQSEWGALYEPDSKSRELIDDIHDNWWLVDLVDNDFVNGDIFK 231
            AVTWGVFP+KEI+QPT++D+ +  IWKDEAFSLW ++W ++YE  S+S ELI +I+D ++LV++VDNDFV+GDI K
Sbjct:  244 AVTWGVFPDKEIMQPTIVDTRSFLIWKDEAFSLWMNDWASIYEAKSESYELIKEIYDTYYLVNVVDNDFVDGDILK 319          
BLAST of mRNA_M-pyrifera_M_contig83744.19550.1 vs. uniprot
Match: A0A8K1CJB9_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CJB9_PYTOL)

HSP 1 Score: 114 bits (285), Expect = 8.990e-28
Identity = 50/79 (63.29%), Postives = 64/79 (81.01%), Query Frame = 1
Query:    1 IAVTWGVFPNKEILQPTVMDSNALPIWKDEAFSLWQSEWGALYEPDSKSRELIDDIHDNWWLVDLVDNDFVNGDIFKPF 237
            IAVTWGVFPNKEILQPT++DS +   WKDEAFSLW   W +LY+ DS + EL+ +IHD+++LV +VDNDFVNG+I+  F
Sbjct:  502 IAVTWGVFPNKEILQPTIVDSTSFVAWKDEAFSLWLKLWASLYDDDSTASELLHEIHDSYFLVSIVDNDFVNGNIWDIF 580          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig83744.19550.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0G4FJW1_9ALVE1.670e-3066.25Methylenetetrahydrofolate reductase n=1 Tax=Chrome... [more]
F4PLP1_CAVFA3.850e-2956.10Methylenetetrahydrofolate reductase n=1 Tax=Cavend... [more]
A0A0D2KCH0_9CHLO3.890e-2965.79Methylenetetrahydrofolate reductase n=1 Tax=Monora... [more]
A0A357HDD7_9BACT7.270e-2960.53Uncharacterized protein n=1 Tax=Candidatus Marinim... [more]
A0A2D4BX93_PYTIN1.280e-2860.76Methylenetetrahydrofolate reductase (NAD(P)H) n=1 ... [more]
A0A2V0P1W0_9CHLO1.740e-2864.47Methylenetetrahydrofolate reductase n=1 Tax=Raphid... [more]
F0ZME6_DICPU3.670e-2856.10Methylenetetrahydrofolate reductase n=1 Tax=Dictyo... [more]
A0A662WW66_9STRA4.470e-2860.76Uncharacterized protein (Fragment) n=1 Tax=Nothoph... [more]
A0A2D5Q091_9BACT5.210e-2861.84Methylenetetrahydrofolate reductase (Fragment) n=1... [more]
A0A8K1CJB9_PYTOL8.990e-2863.29Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig83744contigM-pyrifera_M_contig83744:269..526 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score116.7
Seed ortholog evalue5.4e-24
Seed eggNOG ortholog36331.EPrPI00000018205
Preferred nameMTHFR1
KEGG rclassRC00081
KEGG koko:K00297,ko:K10901
KEGG ReactionR01224,R07168
KEGG Pathwayko00670,ko00720,ko01100,ko01120,ko01200,ko01523,ko03440,ko03460,map00670,map00720,map01100,map01120,map01200,map01523,map03440,map03460
KEGG ModuleM00295,M00377,M00414
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004489,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006730,GO:0008150,GO:0008152,GO:0009987,GO:0016491,GO:0016645,GO:0016646,GO:0044237,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0055114
EggNOG free text desc.Methylenetetrahydrofolate reductase
EggNOG OGs1MCP0@121069,COG0685@1,KOG0564@2759
EC1.5.1.20,3.6.4.12
COG Functional cat.E
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03032,ko03400
Exons1
Model size258
Cds size258
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816745.1084464-CDS-M-pyrifera_M_contig83744:268..5261622816745.1084464-CDS-M-pyrifera_M_contig83744:268..526Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83744 269..526 +
1692278152.9457474-CDS-M-pyrifera_M_contig83744:268..5261692278152.9457474-CDS-M-pyrifera_M_contig83744:268..526Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83744 269..526 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig83744.19550.1prot_M-pyrifera_M_contig83744.19550.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig83744 269..526 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig83744.19550.1

>prot_M-pyrifera_M_contig83744.19550.1 ID=prot_M-pyrifera_M_contig83744.19550.1|Name=mRNA_M-pyrifera_M_contig83744.19550.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=86bp
IAVTWGVFPNKEILQPTVMDSNALPIWKDEAFSLWQSEWGALYEPDSKSR
ELIDDIHDNWWLVDLVDNDFVNGDIFKPFFELLWS*
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mRNA from alignment at M-pyrifera_M_contig83744:269..526+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig83744.19550.1 ID=mRNA_M-pyrifera_M_contig83744.19550.1|Name=mRNA_M-pyrifera_M_contig83744.19550.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=258bp|location=Sequence derived from alignment at M-pyrifera_M_contig83744:269..526+ (Macrocystis pyrifera P11B4 male)
ATCGCCGTCACTTGGGGTGTCTTCCCCAACAAGGAAATCCTCCAGCCCAC GGTGATGGATAGCAACGCCCTCCCAATTTGGAAGGACGAGGCGTTCAGCC TCTGGCAGTCCGAGTGGGGCGCTCTCTACGAGCCTGATAGCAAGAGCAGA GAGCTTATCGACGACATCCATGACAACTGGTGGCTGGTTGACCTCGTGGA CAACGACTTCGTCAACGGCGACATCTTCAAGCCTTTCTTCGAGCTTCTAT GGTCGTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig83744:269..526+

>mRNA_M-pyrifera_M_contig83744.19550.1 ID=mRNA_M-pyrifera_M_contig83744.19550.1|Name=mRNA_M-pyrifera_M_contig83744.19550.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=516bp|location=Sequence derived from alignment at M-pyrifera_M_contig83744:269..526+ (Macrocystis pyrifera P11B4 male)
ATCGCCGTCACTTGGGGTGTCTTCCCCAACAAGGAAATCCTCCAGCCCAC
GGTGATGGATAGCAACGCCCTCCCAATTTGGAAGGACGAGGCGTTCAGCC
TCTGGCAGTCCGAGTGGGGCGCTCTCTACGAGCCTGATAGCAAGAGCAGA
GAGCTTATCGACGACATCCATGACAACTGGTGGCTGGTTGACCTCGTGGA
CAACGACTTCGTCAACGGCGACATCTTCAAGCCTTTCTTCGAGCTTCTAT
GGTCGTAGATCGCCGTCACTTGGGGTGTCTTCCCCAACAAGGAAATCCTC
CAGCCCACGGTGATGGATAGCAACGCCCTCCCAATTTGGAAGGACGAGGC
GTTCAGCCTCTGGCAGTCCGAGTGGGGCGCTCTCTACGAGCCTGATAGCA
AGAGCAGAGAGCTTATCGACGACATCCATGACAACTGGTGGCTGGTTGAC
CTCGTGGACAACGACTTCGTCAACGGCGACATCTTCAAGCCTTTCTTCGA
GCTTCTATGGTCGTAG
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