mRNA_M-pyrifera_M_contig83255.19446.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig83255.19446.1
Unique NamemRNA_M-pyrifera_M_contig83255.19446.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig83255.19446.1 vs. uniprot
Match: B3RLV7_TRIAD (Cytochrome b5 heme-binding domain-containing protein n=2 Tax=Trichoplax TaxID=10227 RepID=B3RLV7_TRIAD)

HSP 1 Score: 130 bits (328), Expect = 8.390e-33
Identity = 65/167 (38.92%), Postives = 105/167 (62.87%), Query Frame = 1
Query:   16 SVATTYTREEVAKHDTLDDLWIVVNDEVLDISDFGRSHPGGAAPLLAMAGSDATDAVIAMHPEYVMKLVNGYSIGKLASPSRPSESLLAYRELRQALKEEGLFVTDSWWYIREAVRLACIFAAMVYVTLFTGN-GWIRAVCGGLLLGVFFQQLAFVGHDLGHNGVTH 513
            ++ T YT EEVAKH+T +DLWI++N++V D++ +   HPGG  P+L + G D TDA    HP++  K ++ + +G L     P++ +  YR L + L+ EG + T   +Y +  + L+ +F   +Y+T++T    W   + G L+LG+F+QQLAFVGHD GH  ++H
Sbjct:   22 NINTPYTIEEVAKHNTENDLWIIINNKVYDVTKWIPYHPGGNLPILNLGGHDVTDAFRGFHPDWAFKKLSAFQVGILTG-YEPNQVVKDYRALDEKLRREGYYKTHYMFYAKLYLWLSFLFCLSMYLTIYTTELKW--QMLGALVLGMFWQQLAFVGHDTGHISISH 185          
BLAST of mRNA_M-pyrifera_M_contig83255.19446.1 vs. uniprot
Match: A7RJB6_NEMVE (Predicted protein n=2 Tax=Nematostella vectensis TaxID=45351 RepID=A7RJB6_NEMVE)

HSP 1 Score: 128 bits (322), Expect = 4.930e-32
Identity = 69/165 (41.82%), Postives = 103/165 (62.42%), Query Frame = 1
Query:   31 YTREEVAKHDTLDDLWIVVNDEVLDISDFGRSHPGGAAPLLAMAGSDATDAVIAMHPEYVM-KLVNGYSIGKLASPSRPS-ESLLA--YRELRQALKEEGLFVTDSWWYIREAVRLACIFAAMVYVTLFTGNGWIRAVCGGLLLGVFFQQLAFVGHDLGHNGVTH 513
            YT +EV +H +  D W+VV D V D+S +   HPGG  P+L MAG + TD   A HP +V  K +  + IGKL    +   E+ L+  + +LRQ + E G   T+ W+YIR A  LA +FA+++Y  +F+ N +I+ V  G+L+ VF+QQ+AF+GHD GH+ + H
Sbjct:   13 YTLDEVKEHCSKGDCWVVVEDSVYDLSKWIGHHPGGELPILYMAGRECTDVFKAFHPAWVFTKKLPAFKIGKLDDTRKEKKETSLSEDFEKLRQEIIEGGGLQTNYWFYIRLASMLALLFASIIYCVVFSNNVYIQ-VAAGILVAVFWQQMAFIGHDAGHHAIFH 176          
BLAST of mRNA_M-pyrifera_M_contig83255.19446.1 vs. uniprot
Match: A0A7S1F4L7_NOCSC (Hypothetical protein n=1 Tax=Noctiluca scintillans TaxID=2966 RepID=A0A7S1F4L7_NOCSC)

HSP 1 Score: 127 bits (320), Expect = 1.970e-31
Identity = 69/169 (40.83%), Postives = 97/169 (57.40%), Query Frame = 1
Query:   16 SVATTYTREEVAKHDTLDDLWIVVNDEVLDISDFGRSHPGGAAPLLAMAGSDATDAVIAMHPEYVMKLVNGYSIGKLASPSRPSESLLAYRELRQALKEEGLFVTDSWWYIREAVRLAC-IFAAMVYVTLFTGNG--WIRAVCGGLLLGVFFQQLAFVGHDLGHNGVTH 513
            S A   T EEV KHD  DD WIV++ +V D++ + +SHPGG  PL   AG DATD   A HP    K +    +G L   + PSE +  +R +RQ L ++G F  + W+ + + V   C + AA+V V    G+   W     GG+LL +F+QQ+A +GHD GHN +TH
Sbjct:   83 SPAPDVTIEEVRKHDQRDDCWIVIDQKVYDVTSWAKSHPGGPLPLWGTAGRDATDHFYAFHPNGTEKWLPPLQVGNLVGYT-PSEVVRDFRAMRQRLIDQGFFEINRWYSLEKYVAFMCMLLAAIVCVLCSAGSSYSWCWTGVGGVLLAMFWQQMAGLGHDCGHNSLTH 250          
BLAST of mRNA_M-pyrifera_M_contig83255.19446.1 vs. uniprot
Match: A0A6P8H3R0_ACTTE (delta(8)-fatty-acid desaturase-like n=1 Tax=Actinia tenebrosa TaxID=6105 RepID=A0A6P8H3R0_ACTTE)

HSP 1 Score: 125 bits (313), Expect = 9.520e-31
Identity = 63/165 (38.18%), Postives = 103/165 (62.42%), Query Frame = 1
Query:   31 YTREEVAKHDTLDDLWIVVNDEVLDISDFGRSHPGGAAPLLAMAGSDATDAVIAMHPEYVM-KLVNGYSIGKLASPS---RPSESLLAYRELRQALKEEGLFVTDSWWYIREAVRLACIFAAMVYVTLFTGNGWIRAVCGGLLLGVFFQQLAFVGHDLGHNGVTH 513
            YT EEV KH T DD W++++ +V ++S + + HPGG  P+  MAG D TD   A HP++V  K +  + IG++   S   + ++    + E+R+ + E+G  VT+  +Y +    LA +FAA++Y  +F+ N   + + G +LL VF+QQ+AF+GHD GH+ +TH
Sbjct:   12 YTSEEVKKHCTKDDCWVIISGQVYNLSKWIQFHPGGELPIRYMAGHDCTDVFKAFHPDWVAEKKLPAFRIGRVKGESDEIKKTKLSEDFEEMRKKIIEDGGLVTNYCFYAKIVSMLALVFAAIIYCVIFSNNVMFQ-IFGAVLLAVFWQQMAFIGHDTGHHAITH 175          
BLAST of mRNA_M-pyrifera_M_contig83255.19446.1 vs. uniprot
Match: A0A0L0H961_SPIPD (Cytochrome b5 heme-binding domain-containing protein n=2 Tax=Spizellomyces TaxID=4815 RepID=A0A0L0H961_SPIPD)

HSP 1 Score: 123 bits (309), Expect = 3.600e-30
Identity = 63/163 (38.65%), Postives = 100/163 (61.35%), Query Frame = 1
Query:   31 YTREEVAKHDTLDDLWIVVNDEVLDISDFGRSHPGGAAPLLAMAGSDATDAVIAMHPEYVM-KLVNGYSIGKLASPSRPSESL-LAYRELRQALKEEGLFVTDSWWYIREAVRLACIFAAMVYVTLFTGNGWIRAVCGGLLLGVFFQQLAFVGHDLGHNGVTH 513
            +TR+E+       ++ +++N +V +++ F   HPGG   +  MAG DATDAV+A HPE V+ K +  + IG+LA  ++ +  L LAYR L   LK EG++ T+ W+Y+REAV++  I+ AM+ + ++    W+      LL    + Q AFV HD GH+G+TH
Sbjct:   19 FTRKEIEDRVKQGEILVIINSKVYNLTKFINHHPGGDLAIWHMAGKDATDAVLAYHPEPVIEKRMPHFCIGELAPNTQHASKLSLAYRRLDAKLKAEGMYETNYWFYVREAVKMLAIWFAMIGLVVWGPQHWMTYAASALLGSTLWHQAAFVAHDAGHSGITH 181          
BLAST of mRNA_M-pyrifera_M_contig83255.19446.1 vs. uniprot
Match: UPI001CF25834 (LOW QUALITY PROTEIN: delta(8)-fatty-acid desaturase 2-like n=1 Tax=Acropora millepora TaxID=45264 RepID=UPI001CF25834)

HSP 1 Score: 121 bits (304), Expect = 1.720e-29
Identity = 67/166 (40.36%), Postives = 100/166 (60.24%), Query Frame = 1
Query:   31 YTREEVAKHDTLDDLWIVVNDEVLDISDFGRSHPGGAAPLLAMAGSDATDAVIAMHPEYVMKL-VNGYSIGKLASPSRPS--ESLLA--YRELRQALKEEGLFVTDSWWYIREAVRLACIFAAMVYVTLFTGNGWIRAVCGGLLLGVFFQQLAFVGHDLGHNGVTH 513
            Y+ EEV KH+T  D W+V++D V D+S +   HPGG  P+  MAG + +D   A H   + +  +  + IG++    + S  ES L   YR+LR+ +  EG   TD W+Y+R+A+ L  IFAA+VY  +F+ N +I+ V     +  F+QQ+AFVGHD GH  +TH
Sbjct:    7 YSFEEVKKHNTKGDYWVVIDDGVYDLSKWAPHHPGGELPIRYMAGRECSDVFKAFHNSSIRETKLPLFKIGEIRDDCKTSIKESSLEQDYRKLREEV--EGCLQTDYWFYVRQAINLGLIFAALVYGVIFSDNVYIQ-VASSFTMAAFWQQMAFVGHDGGHMAITH 169          
BLAST of mRNA_M-pyrifera_M_contig83255.19446.1 vs. uniprot
Match: A0A7S1B6D4_9STRA (Hypothetical protein (Fragment) n=2 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1B6D4_9STRA)

HSP 1 Score: 119 bits (298), Expect = 3.680e-28
Identity = 66/165 (40.00%), Postives = 94/165 (56.97%), Query Frame = 1
Query:   31 YTREEVAKHDTLDDLWIVVNDEVLDISDFGRSHPGGAAPLLAMAGSDATDAVIAMHPEYVMK-LVNGYSIGKLASPSRPSESLLAYRELRQALKEEGLFVTDSWWYIREAVRLACIFAAMVYVTLF---TGNGWIRAVCGGLLLGVFFQQLAFVGHDLGHNGVTH 513
            YT   VAKH T DD WI++ + V D++ F   HPGG  P++ +AG D TD     H   V K ++  Y +G++     P E +  +R++RQ L   GLF TD  +Y R    L  +F   V +TL+   + N     + G  ++GVF+QQLA +GHDLGH+GVTH
Sbjct:  174 YTTAAVAKHCTKDDCWIIIGEMVYDVTSFIDQHPGGIGPMVNLAGKDCTDVFANYHSARVYKYMLPRYIVGEVVGVEVP-EHVKDFRKVRQELLRRGLFETDYRFYFRLGCWLGFLFTGAVCLTLWPSNSSNALSYRLVGAAMMGVFWQQLAGLGHDLGHSGVTH 337          
BLAST of mRNA_M-pyrifera_M_contig83255.19446.1 vs. uniprot
Match: A0A1Y1HRW9_KLENI (Sphingobase-D8 Desaturase n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1HRW9_KLENI)

HSP 1 Score: 118 bits (296), Expect = 3.740e-28
Identity = 63/158 (39.87%), Postives = 95/158 (60.13%), Query Frame = 1
Query:   40 EEVAKHDTLDDLWIVVNDEVLDISDFGRSHPGGAAPLLAMAGSDATDAVIAMHPEYVMKL-VNGYSIGKLASPSRPSESLLAYRELRQALKEEGLFVTDSWWYIREAVRLACIFAAMVYVTLFTGNGWIRAVCGGLLLGVFFQQLAFVGHDLGHNGVT 510
            EE+ +H T  D WI +  +V + +++ + HPGG   +L MAG DATDA  A H   V K  +  + +G LA+ + PS+ L  YR+L + L+   LF T   +Y+ +A+ +A +FA +V     T + W+  +  GLLLG+F+QQ AFVGHD GH  +T
Sbjct:   55 EELERHCTASDCWIAIEGQVYEATEWLKLHPGGDICILNMAGQDATDAFRAFHKPMVSKFHLPRFHVGPLAAAAPPSKMLQEYRQLAKDLESSELFQTRPSFYLSKALVIAALFALVVAGVTLTSSPWVH-LASGLLLGLFWQQCAFVGHDTGHLSIT 211          
BLAST of mRNA_M-pyrifera_M_contig83255.19446.1 vs. uniprot
Match: A0A7S2BE31_9STRA (Hypothetical protein n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2BE31_9STRA)

HSP 1 Score: 118 bits (295), Expect = 5.900e-28
Identity = 65/161 (40.37%), Postives = 93/161 (57.76%), Query Frame = 1
Query:   34 TREEVAKHDTLDDLWIVVNDEVLDISDFGRSHPGGAAPLLAMAGSDATDAVIAMHPEYVMK-LVNGYSIGKLASPSRPSESLLAYRELRQALKEEGLFVTDSWWYIREAVRLACIFAAMVYVTLFTGNGWIRAVCGGLLLGVFFQQLAFVGHDLGHNGVTH 513
            T  E AKH+  DDLW++V+ +  D++ +   HPGG  P++ + G DATDA    HP  V K ++  Y IG +    R +E    +R +RQ L  EG+F T   +Y +     A + A  VY TL   +  +  + G +LLG F+QQ+A VGHD+GHNGVTH
Sbjct:   64 TMAEAAKHNKRDDLWVIVDGKCYDVTRYVEHHPGGWLPMVNLGGKDATDAFANYHPARVYKNMLPQYYIGDVVD-YRETEFQKGHRAIRQRLLAEGMFETRMSYYYKLTAWFAALLAGAVYCTLACTSFAVH-MAGAVLLGAFWQQMAGVGHDIGHNGVTH 222          
BLAST of mRNA_M-pyrifera_M_contig83255.19446.1 vs. uniprot
Match: A0A7S4EWT8_CHRCT (Hypothetical protein n=3 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4EWT8_CHRCT)

HSP 1 Score: 118 bits (295), Expect = 9.010e-28
Identity = 65/170 (38.24%), Postives = 99/170 (58.24%), Query Frame = 1
Query:    7 EGVSVATTYTREEVAKHDTLDDLWIVVNDEVLDISDFGRSHPGGAAPLLAMAGSDATDAVIAMHPEYVMK-LVNGYSIGKLASPSRPSESLLAYRELRQALKEEGLFVTDSWWYIREAVRLACIFAAMVYVTLFTGNGWIRAVCGGLLLGVFFQQLAFVGHDLGHNGVTH 513
            EG S   +YT  +VA  +T  +LW+ ++  V D++ F   HPGGA P++ MAG D TD     HP  V K ++  Y IG++ S  +    +  +R +RQ L   GLF TD  +Y +    LA +F   +++TL + +  +    G  ++G+F+QQLA VGHDLGH+GV+H
Sbjct:  141 EGQSKLASYTMAQVAARNTSSELWVAIDGYVYDLTRFVLKHPGGALPIMNMAGKDCTDVFANYHPARVYKTMLPAYLIGQV-SDYQLYPHVAEFRAIRQELLRRGLFETDLRFYAKLGAFLAFLFCTALWLTL-SCSSTVAHAAGAAVMGIFWQQLAGVGHDLGHSGVSH 308          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig83255.19446.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
B3RLV7_TRIAD8.390e-3338.92Cytochrome b5 heme-binding domain-containing prote... [more]
A7RJB6_NEMVE4.930e-3241.82Predicted protein n=2 Tax=Nematostella vectensis T... [more]
A0A7S1F4L7_NOCSC1.970e-3140.83Hypothetical protein n=1 Tax=Noctiluca scintillans... [more]
A0A6P8H3R0_ACTTE9.520e-3138.18delta(8)-fatty-acid desaturase-like n=1 Tax=Actini... [more]
A0A0L0H961_SPIPD3.600e-3038.65Cytochrome b5 heme-binding domain-containing prote... [more]
UPI001CF258341.720e-2940.36LOW QUALITY PROTEIN: delta(8)-fatty-acid desaturas... [more]
A0A7S1B6D4_9STRA3.680e-2840.00Hypothetical protein (Fragment) n=2 Tax=Corethron ... [more]
A0A1Y1HRW9_KLENI3.740e-2839.87Sphingobase-D8 Desaturase n=1 Tax=Klebsormidium ni... [more]
A0A7S2BE31_9STRA5.900e-2840.37Hypothetical protein n=1 Tax=Florenciella parvula ... [more]
A0A7S4EWT8_CHRCT9.010e-2838.24Hypothetical protein n=3 Tax=Chrysotila carterae T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig83255contigM-pyrifera_M_contig83255:385..897 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score131.0
Seed ortholog evalue5.5e-28
Seed eggNOG ortholog10228.TriadP36979
KEGG koko:K21737
Hectar predicted targeting categoryother localisation
EggNOG free text desc.omega-6 fatty acid desaturase activity
EggNOG OGs38B7V@33154,3B9GD@33208,COG3239@1,KOG4232@2759
EC1.14.19.38,1.14.19.47
COG Functional cat.I
Best tax levelMetazoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000
Exons1
Model size513
Cds size513
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816739.4272714-CDS-M-pyrifera_M_contig83255:384..8971622816739.4272714-CDS-M-pyrifera_M_contig83255:384..897Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83255 385..897 +
1692278149.784515-CDS-M-pyrifera_M_contig83255:384..8971692278149.784515-CDS-M-pyrifera_M_contig83255:384..897Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83255 385..897 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig83255.19446.1prot_M-pyrifera_M_contig83255.19446.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig83255 385..897 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig83255.19446.1

>prot_M-pyrifera_M_contig83255.19446.1 ID=prot_M-pyrifera_M_contig83255.19446.1|Name=mRNA_M-pyrifera_M_contig83255.19446.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=171bp
MPEGVSVATTYTREEVAKHDTLDDLWIVVNDEVLDISDFGRSHPGGAAPL
LAMAGSDATDAVIAMHPEYVMKLVNGYSIGKLASPSRPSESLLAYRELRQ
ALKEEGLFVTDSWWYIREAVRLACIFAAMVYVTLFTGNGWIRAVCGGLLL
GVFFQQLAFVGHDLGHNGVTH
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mRNA from alignment at M-pyrifera_M_contig83255:385..897+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig83255.19446.1 ID=mRNA_M-pyrifera_M_contig83255.19446.1|Name=mRNA_M-pyrifera_M_contig83255.19446.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=513bp|location=Sequence derived from alignment at M-pyrifera_M_contig83255:385..897+ (Macrocystis pyrifera P11B4 male)
ATGCCCGAAGGAGTTTCCGTCGCTACGACGTACACTCGAGAAGAAGTAGC CAAGCATGACACATTGGACGACCTTTGGATCGTAGTGAACGACGAGGTGC TCGATATTTCAGATTTCGGGCGAAGCCATCCAGGCGGCGCAGCGCCGTTA CTCGCAATGGCAGGCTCGGATGCCACCGACGCTGTCATTGCTATGCATCC TGAGTATGTCATGAAGCTCGTGAATGGGTACAGCATCGGCAAGCTTGCGT CTCCAAGCCGCCCATCAGAATCGCTTTTGGCGTATCGTGAATTGCGCCAA GCGCTCAAGGAAGAAGGCCTTTTCGTGACCGACTCTTGGTGGTACATCCG GGAGGCAGTTCGTTTGGCTTGCATTTTTGCAGCGATGGTCTATGTCACGC TCTTTACAGGCAATGGCTGGATTCGAGCCGTCTGTGGAGGCCTCTTGTTG GGAGTTTTCTTCCAGCAACTCGCTTTTGTGGGACATGACCTCGGTCACAA TGGAGTGACACAC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig83255:385..897+

>mRNA_M-pyrifera_M_contig83255.19446.1 ID=mRNA_M-pyrifera_M_contig83255.19446.1|Name=mRNA_M-pyrifera_M_contig83255.19446.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1026bp|location=Sequence derived from alignment at M-pyrifera_M_contig83255:385..897+ (Macrocystis pyrifera P11B4 male)
ATGCCCGAAGGAGTTTCCGTCGCTACGACGTACACTCGAGAAGAAGTAGC
CAAGCATGACACATTGGACGACCTTTGGATCGTAGTGAACGACGAGGTGC
TCGATATTTCAGATTTCGGGCGAAGCCATCCAGGCGGCGCAGCGCCGTTA
CTCGCAATGGCAGGCTCGGATGCCACCGACGCTGTCATTGCTATGCATCC
TGAGTATGTCATGAAGCTCGTGAATGGGTACAGCATCGGCAAGCTTGCGT
CTCCAAGCCGCCCATCAGAATCGCTTTTGGCGTATCGTGAATTGCGCCAA
GCGCTCAAGGAAGAAGGCCTTTTCGTGACCGACTCTTGGTGGTACATCCG
GGAGGCAGTTCGTTTGGCTTGCATTTTTGCAGCGATGGTCTATGTCACGC
TCTTTACAGGCAATGGCTGGATTCGAGCCGTCTGTGGAGGCCTCTTGTTG
GGAGTTTTCTTCCAGCAACTCGCTTTTGTGGGACATGACCTCGGTCACAA
TGGAGTGACACACATGCCCGAAGGAGTTTCCGTCGCTACGACGTACACTC
GAGAAGAAGTAGCCAAGCATGACACATTGGACGACCTTTGGATCGTAGTG
AACGACGAGGTGCTCGATATTTCAGATTTCGGGCGAAGCCATCCAGGCGG
CGCAGCGCCGTTACTCGCAATGGCAGGCTCGGATGCCACCGACGCTGTCA
TTGCTATGCATCCTGAGTATGTCATGAAGCTCGTGAATGGGTACAGCATC
GGCAAGCTTGCGTCTCCAAGCCGCCCATCAGAATCGCTTTTGGCGTATCG
TGAATTGCGCCAAGCGCTCAAGGAAGAAGGCCTTTTCGTGACCGACTCTT
GGTGGTACATCCGGGAGGCAGTTCGTTTGGCTTGCATTTTTGCAGCGATG
GTCTATGTCACGCTCTTTACAGGCAATGGCTGGATTCGAGCCGTCTGTGG
AGGCCTCTTGTTGGGAGTTTTCTTCCAGCAACTCGCTTTTGTGGGACATG
ACCTCGGTCACAATGGAGTGACACAC
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