mRNA_M-pyrifera_M_contig83191.19432.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig83191.19432.1
Unique NamemRNA_M-pyrifera_M_contig83191.19432.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig83191.19432.1 vs. uniprot
Match: A0A7S0K6I8_CAFRO (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 (Fragment) n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A7S0K6I8_CAFRO)

HSP 1 Score: 155 bits (392), Expect = 2.900e-44
Identity = 75/129 (58.14%), Postives = 104/129 (80.62%), Query Frame = 1
Query:    7 EMQTSGEMSL--ISGYDSWGFKVNDVHMRGSVLVFPRFSLLWDVQTLEDISPSSLSPVHLL-EPAIRLLLVGTGETMRNINPELYGYFSRRGVAIEPMTNAHAMSTFNLLVREGRDVAAALLSPEPLTR 384
            +M T+ +  L  +  +D +GF+VN+++MRGSV+  P+F+LLWDVQ + DISP SLSPV +L EP IR+LL+GTG+ MRN+NP L+G+FSRRG+A+E M+  HA+STFN+L REGR VAAALLS +P++R
Sbjct:   34 DMMTNRDAGLTDLQSWDEFGFRVNNIYMRGSVVCLPKFTLLWDVQHVSDISPESLSPVLMLREPRIRILLIGTGKKMRNVNPALFGWFSRRGIAVEHMSTKHALSTFNVLNREGRGVAAALLSRKPVSR 162          
BLAST of mRNA_M-pyrifera_M_contig83191.19432.1 vs. uniprot
Match: A0A5A8CUM3_CAFRO (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 n=2 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CUM3_CAFRO)

HSP 1 Score: 154 bits (390), Expect = 2.410e-43
Identity = 75/128 (58.59%), Postives = 103/128 (80.47%), Query Frame = 1
Query:   10 MQTSGEMSL--ISGYDSWGFKVNDVHMRGSVLVFPRFSLLWDVQTLEDISPSSLSPVHLL-EPAIRLLLVGTGETMRNINPELYGYFSRRGVAIEPMTNAHAMSTFNLLVREGRDVAAALLSPEPLTR 384
            M T+ +  L  +  +D +GF+VN+++MRGSV+  P+F+LLWDVQ + DISP SLSPV +L EP IR+LL+GTG+ MRN+NP L+G+FSRRG+A+E M+  HA+STFN+L REGR VAAALLS +P++R
Sbjct:    1 MMTNRDAGLTDLQSWDEFGFRVNNIYMRGSVVCLPKFTLLWDVQHVSDISPESLSPVLMLREPRIRILLIGTGKKMRNVNPALFGWFSRRGIAVEHMSTKHALSTFNVLNREGRGVAAALLSRKPVSR 128          
BLAST of mRNA_M-pyrifera_M_contig83191.19432.1 vs. uniprot
Match: A0A813BYD1_9DINO (Ndufaf3 protein (Fragment) n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813BYD1_9DINO)

HSP 1 Score: 148 bits (373), Expect = 3.040e-43
Identity = 67/118 (56.78%), Postives = 96/118 (81.36%), Query Frame = 1
Query:   31 SLISGYDSWGFKVNDVHMRGSVLVFPRFSLLWDVQTLEDISPSSLSPVHLLEPAIRLLLVGTGETMRNINPELYGYFSRRGVAIEPMTNAHAMSTFNLLVREGRDVAAALLSPEPLTR 384
            +++ G+D +G+KVN ++MRGSVL FP F+LLWDVQ + D++  SLSPV +  P +R+LL+GTGE M+N+NP LYG+FS++GVA+E M++ +AM+TFN+L +EGR  AAALLS +PL R
Sbjct:    6 TVVDGWDDYGYKVNGIYMRGSVLCFPSFTLLWDVQQIADVTHESLSPVLMFAPRVRVLLIGTGEEMQNVNPLLYGHFSKQGVAVEHMSSDNAMATFNVLNQEGRPAAAALLSRKPLPR 123          
BLAST of mRNA_M-pyrifera_M_contig83191.19432.1 vs. uniprot
Match: A0A482RKF2_9ARCH (Uncharacterized protein (Fragment) n=1 Tax=archaeon TaxID=1906665 RepID=A0A482RKF2_9ARCH)

HSP 1 Score: 113 bits (282), Expect = 1.860e-27
Identity = 70/187 (37.43%), Postives = 91/187 (48.66%), Query Frame = 1
Query:   31 SLISGYDSWGFKVNDVHMRGSVLVFPRFSLLWDVQTLEDISPSSLSPVHLLEP----------------------------------AIRLLLVGTGETMRNINPELYGYFSRRGVAIEPMTN-----------------------------------AHAMSTFNLLVREGRDVAAALLSPEPLTR 384
            S I G D++GF VN + MRGSVLVFP F+LLWDVQ    I P SL+PVH++ P                                  +  L+LVGTG+   N+NP LY YFSRRG+A+EPM+                                    AHA++ FN+L  EGR VAAAL+S +P++R
Sbjct:   99 SRIGGADAYGFLVNGIAMRGSVLVFPTFALLWDVQHAHAICPRSLAPVHMVSPQPGANLPPVRRRARMTRFHLQHSHTPRVLVLWANSAELVLVGTGDQSVNLNPSLYAYFSRRGIAVEPMSTVGSSTSARTCQHGTSHHGRSSPAPDPRAVRSRTLQAHAIAEFNVLNEEGRMVAAALISRQPMSR 285          
BLAST of mRNA_M-pyrifera_M_contig83191.19432.1 vs. uniprot
Match: A0A0D2WX21_CAPO3 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 n=2 Tax=Capsaspora owczarzaki (strain ATCC 30864) TaxID=595528 RepID=A0A0D2WX21_CAPO3)

HSP 1 Score: 101 bits (251), Expect = 6.440e-25
Identity = 50/116 (43.10%), Postives = 74/116 (63.79%), Query Frame = 1
Query:   34 LISGYDSWGFKVNDVHMRGSVLVFPRFSLLWDVQTLEDISPSSLSPVHLLEPAIRLLLVGTGETMRNINPELYGYFSRRGVAIEPMTNAHAMSTFNLLVREGRDVAAALLSPEPLT 381
            LI  + S GF VN V   GS+ +FPR  LLW+V+++E+++P SL+  HL+ P I LLL+GTG  +  I+P +      + +++E +   HA +TFN L +EGR VAAAL+   P T
Sbjct:   21 LIDSFSSMGFTVNGVTYVGSMALFPRVPLLWNVKSVEELTPESLTLFHLVNPKIELLLIGTGSKIETIDPAVRQMLKEKRISLEVLDTPHAATTFNFLSQEGRLVAAALIPIAPET 136          
BLAST of mRNA_M-pyrifera_M_contig83191.19432.1 vs. uniprot
Match: A0A7S0NEW1_9CRYP (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 n=1 Tax=Hanusia phi TaxID=3032 RepID=A0A7S0NEW1_9CRYP)

HSP 1 Score: 102 bits (253), Expect = 3.290e-24
Identity = 46/119 (38.66%), Postives = 80/119 (67.23%), Query Frame = 1
Query:    7 EMQTSGEMSLISGYDSWGFKVNDVHMRGSVLVFPRFSLLWDVQTLEDISPSSLSPVHLLEPAIRLLLVGTGETMRNINPELYGYFSRRGVAIEPMTNAHAMSTFNLLVREGRDVAAALL 363
            +  T  +++++  Y+ +G  VN V + G+VL+ P  S LW+V +LEDI+P SL  + ++ P I + ++GTG+  R  +PEL  +FS +G++++ M + +A +TFN+L +EGR+ AAA L
Sbjct:   95 DSSTLNDLTVVQSYNQYGVVVNGVILPGAVLLLPETSFLWNVNSLEDITPDSLKVLTMVTPRIEVCILGTGKRTRRPSPELISWFSSQGISLDFMDSVNAFATFNVLTQEGRNAAAACL 213          
BLAST of mRNA_M-pyrifera_M_contig83191.19432.1 vs. uniprot
Match: L1J926_GUITC (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1J926_GUITC)

HSP 1 Score: 92.8 bits (229), Expect = 1.150e-21
Identity = 44/115 (38.26%), Postives = 74/115 (64.35%), Query Frame = 1
Query:   28 MSLISGYDSWGFKVNDVHMRGSVLVFPRFSLLWDVQTLEDISPSSLSPVHLLEPAIRLLLVGTGETMRNINPELYGYFSRRGVAIEPMTNAHAMSTFNLLVREGRDVAAALLSPE 372
            M+++  Y+ +G  VN+V + G+VL+ P+ S LW+V ++EDI+  SL  + +L P I + ++GTG   R    EL  +F  +G++++ M + +A +TFN+L +EGR  AAA L  E
Sbjct:    1 MTVVQSYNQYGIVVNNVILPGAVLLLPKTSFLWNVNSMEDITVDSLKVLTMLSPRIEVCILGTGNRTRRPPAELISWFGDQGISLDFMDSVNAFATFNVLTQEGRVAAAACLPVE 115          
BLAST of mRNA_M-pyrifera_M_contig83191.19432.1 vs. uniprot
Match: NDUF3_XENTR (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3 n=2 Tax=Xenopus TaxID=8353 RepID=NDUF3_XENTR)

HSP 1 Score: 92.4 bits (228), Expect = 5.330e-21
Identity = 47/118 (39.83%), Postives = 70/118 (59.32%), Query Frame = 1
Query:   19 SGEMSLISGYDSWGFKVNDVHMRGSVLVFPRFSLLWDVQTLEDISPSSLSPVHLLEPAIRLLLVGTGETMRNINPELYGYFSRRGVAIEPMTNAHAMSTFNLLVREGRDVAAALLSPE 372
            S E   I  Y S GF +N   + G   V P+  L W+V + +DI+  SLS  H+L P I +L+VGTG+ +  ++P +  +  ++GVA+E    A+A +TFN LV E R  AAAL+ P+
Sbjct:   59 SAEFMFIETYSSQGFIINGDKVVGPCAVIPKAILQWNVGSYKDINLESLSLFHMLSPRIEILVVGTGDRVERLDPNILKFMRQKGVAVEVQDTANACATFNFLVSERRITAAALIPPQ 176          
BLAST of mRNA_M-pyrifera_M_contig83191.19432.1 vs. uniprot
Match: V6EYG3_MAGGM (Uncharacterized protein n=2 Tax=Magnetospirillum gryphiswaldense TaxID=55518 RepID=V6EYG3_MAGGM)

HSP 1 Score: 90.5 bits (223), Expect = 6.690e-21
Identity = 43/116 (37.07%), Postives = 68/116 (58.62%), Query Frame = 1
Query:   19 SGEMSLISGYDSWGFKVNDVHMRGSVLVFPRFSLLWDVQTLEDISPSSLSPVHLLEPAIRLLLVGTGETMRNINPELYGYFSRRGVAIEPMTNAHAMSTFNLLVREGRDVAAALLS 366
            S E  LI GY   GF ++ +   GSVL+ PR +L W VQ+  D++  +L P+   +P   +L++G G +M  +   L       G+ +EPM +  A  T+N+L+ EGRDVAAA+++
Sbjct:    8 SSEFQLIGGYGDGGFSISGIRHEGSVLILPRRTLSWTVQSASDVALENLQPIIGADPRPAILILGCGRSMAPVPRALRDALRGHGIVVEPMDSGAACRTYNVLLTEGRDVAAAIIA 123          
BLAST of mRNA_M-pyrifera_M_contig83191.19432.1 vs. uniprot
Match: UPI00187BB33E (Mth938-like domain-containing protein n=1 Tax=Sneathiella sp. P13V-1 TaxID=2697366 RepID=UPI00187BB33E)

HSP 1 Score: 90.5 bits (223), Expect = 6.870e-21
Identity = 44/111 (39.64%), Postives = 70/111 (63.06%), Query Frame = 1
Query:   34 LISGYDSWGFKVNDVHMRGSVLVFPRFSLLWDVQTLEDISPSSLSPVHLLEPAIRLLLVGTGETMRNINPELYGYFSRRGVAIEPMTNAHAMSTFNLLVREGRDVAAALLS 366
            LI+GY   GF+++ V   GSVLVFP+ +  WD+ ++E+++   L  V   +PA+ +LL+G G  M  I+  +   F   G+ I+ M +  AM T+N+L+ EGR VAAAL++
Sbjct:   14 LINGYGDGGFRISGVRHEGSVLVFPKETQAWDLTSIEELTLDHLKAVKEADPAVEILLIGCGANMAFIDEAIRNDFRAAGIVIDSMDSGAAMRTYNVLLLEGRRVAAALIA 124          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig83191.19432.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S0K6I8_CAFRO2.900e-4458.14NADH dehydrogenase [ubiquinone] 1 alpha subcomplex... [more]
A0A5A8CUM3_CAFRO2.410e-4358.59NADH dehydrogenase [ubiquinone] 1 alpha subcomplex... [more]
A0A813BYD1_9DINO3.040e-4356.78Ndufaf3 protein (Fragment) n=1 Tax=Symbiodinium sp... [more]
A0A482RKF2_9ARCH1.860e-2737.43Uncharacterized protein (Fragment) n=1 Tax=archaeo... [more]
A0A0D2WX21_CAPO36.440e-2543.10NADH dehydrogenase [ubiquinone] 1 alpha subcomplex... [more]
A0A7S0NEW1_9CRYP3.290e-2438.66NADH dehydrogenase [ubiquinone] 1 alpha subcomplex... [more]
L1J926_GUITC1.150e-2138.26NADH dehydrogenase [ubiquinone] 1 alpha subcomplex... [more]
NDUF3_XENTR5.330e-2139.83NADH dehydrogenase [ubiquinone] 1 alpha subcomplex... [more]
V6EYG3_MAGGM6.690e-2137.07Uncharacterized protein n=2 Tax=Magnetospirillum g... [more]
UPI00187BB33E6.870e-2139.64Mth938-like domain-containing protein n=1 Tax=Snea... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig83191contigM-pyrifera_M_contig83191:17..400 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score105.5
Seed ortholog evalue1.8e-20
Seed eggNOG ortholog7668.SPU_011847-tr
Preferred nameNDUFAF3
KEGG koko:K09008
KEGG Pathwayko04714,map04714
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0006996,GO:0007005,GO:0008150,GO:0009987,GO:0010257,GO:0016020,GO:0016043,GO:0019866,GO:0022607,GO:0031090,GO:0031966,GO:0031967,GO:0031975,GO:0032981,GO:0033108,GO:0034622,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043933,GO:0044085,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0065003,GO:0071840
EggNOG free text desc.mitochondrial respiratory chain complex I assembly
EggNOG OGs3A05D@33154,3BQ2C@33208,3D0PQ@33213,KOG3363@1,KOG3363@2759
COG Functional cat.S
Best tax levelBilateria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03029
Exons1
Model size384
Cds size375
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816738.841484-UTR-M-pyrifera_M_contig83191:16..251622816738.841484-UTR-M-pyrifera_M_contig83191:16..25Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig83191 17..25 +
1692278149.5195873-UTR-M-pyrifera_M_contig83191:16..251692278149.5195873-UTR-M-pyrifera_M_contig83191:16..25Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig83191 17..25 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816738.8538308-CDS-M-pyrifera_M_contig83191:25..4001622816738.8538308-CDS-M-pyrifera_M_contig83191:25..400Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83191 26..400 +
1692278149.5318213-CDS-M-pyrifera_M_contig83191:25..4001692278149.5318213-CDS-M-pyrifera_M_contig83191:25..400Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83191 26..400 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig83191.19432.1prot_M-pyrifera_M_contig83191.19432.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig83191 26..400 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig83191.19432.1

>prot_M-pyrifera_M_contig83191.19432.1 ID=prot_M-pyrifera_M_contig83191.19432.1|Name=mRNA_M-pyrifera_M_contig83191.19432.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=125bp
MQTSGEMSLISGYDSWGFKVNDVHMRGSVLVFPRFSLLWDVQTLEDISPS
SLSPVHLLEPAIRLLLVGTGETMRNINPELYGYFSRRGVAIEPMTNAHAM
STFNLLVREGRDVAAALLSPEPLTR
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mRNA from alignment at M-pyrifera_M_contig83191:17..400+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig83191.19432.1 ID=mRNA_M-pyrifera_M_contig83191.19432.1|Name=mRNA_M-pyrifera_M_contig83191.19432.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=384bp|location=Sequence derived from alignment at M-pyrifera_M_contig83191:17..400+ (Macrocystis pyrifera P11B4 male)
TATGACGAAATGCAGACATCTGGAGAGATGTCACTGATATCTGGATATGA TTCTTGGGGGTTTAAGGTGAATGATGTGCACATGCGTGGCTCTGTATTGG TGTTTCCCCGGTTTTCTTTGTTGTGGGATGTGCAAACTCTGGAAGACATC AGTCCTTCATCCCTTTCCCCAGTGCATCTCCTAGAACCTGCAATCCGTCT CTTGTTGGTGGGAACAGGTGAAACAATGCGCAACATTAACCCTGAATTAT ACGGATATTTTTCTCGACGCGGTGTAGCAATCGAGCCCATGACCAATGCA CATGCCATGTCAACCTTCAACCTCTTGGTGCGTGAAGGGCGAGATGTAGC AGCTGCTCTTCTCTCCCCTGAGCCCCTCACAAGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig83191:17..400+

>mRNA_M-pyrifera_M_contig83191.19432.1 ID=mRNA_M-pyrifera_M_contig83191.19432.1|Name=mRNA_M-pyrifera_M_contig83191.19432.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=750bp|location=Sequence derived from alignment at M-pyrifera_M_contig83191:17..400+ (Macrocystis pyrifera P11B4 male)
ATGCAGACATCTGGAGAGATGTCACTGATATCTGGATATGATTCTTGGGG
GTTTAAGGTGAATGATGTGCACATGCGTGGCTCTGTATTGGTGTTTCCCC
GGTTTTCTTTGTTGTGGGATGTGCAAACTCTGGAAGACATCAGTCCTTCA
TCCCTTTCCCCAGTGCATCTCCTAGAACCTGCAATCCGTCTCTTGTTGGT
GGGAACAGGTGAAACAATGCGCAACATTAACCCTGAATTATACGGATATT
TTTCTCGACGCGGTGTAGCAATCGAGCCCATGACCAATGCACATGCCATG
TCAACCTTCAACCTCTTGGTGCGTGAAGGGCGAGATGTAGCAGCTGCTCT
TCTCTCCCCTGAGCCCCTCACAAGAATGCAGACATCTGGAGAGATGTCAC
TGATATCTGGATATGATTCTTGGGGGTTTAAGGTGAATGATGTGCACATG
CGTGGCTCTGTATTGGTGTTTCCCCGGTTTTCTTTGTTGTGGGATGTGCA
AACTCTGGAAGACATCAGTCCTTCATCCCTTTCCCCAGTGCATCTCCTAG
AACCTGCAATCCGTCTCTTGTTGGTGGGAACAGGTGAAACAATGCGCAAC
ATTAACCCTGAATTATACGGATATTTTTCTCGACGCGGTGTAGCAATCGA
GCCCATGACCAATGCACATGCCATGTCAACCTTCAACCTCTTGGTGCGTG
AAGGGCGAGATGTAGCAGCTGCTCTTCTCTCCCCTGAGCCCCTCACAAGA
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