mRNA_M-pyrifera_M_contig83181.19429.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig83181.19429.1
Unique NamemRNA_M-pyrifera_M_contig83181.19429.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig83181.19429.1 vs. uniprot
Match: A0A7S0P836_CAFRO (Vacuolar protein sorting-associated protein 51 homolog (Fragment) n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A7S0P836_CAFRO)

HSP 1 Score: 157 bits (397), Expect = 8.250e-43
Identity = 80/171 (46.78%), Postives = 118/171 (69.01%), Query Frame = 1 
Query:    4 MRALLSSYYGVEDDADXXXXXXXARRDIDASGFDSAAHVSDVLRERPLPEVIAYHKRLSSEARGLDTGMQDLVYENYSKFLSATETVRVMRDRVGEIQAAMGRLEGRMALLGEASARVDGALKGNRERVEKLVAVRGLLDQLDFLFELPRRLQRAVALGAHSQAVRYYKAA 516
            M  LL+S+YG +           A+ DID+  FD+ A+  D+L ++ LP++IAY + L+ +AR LD+ MQ+LVYENY+KF+ ATET+R MRD VGE++A M  L   +   G  SA++ G+L  NR ++E+L  VRGL+ +L+FLF+LP RL+  + LGAH QAV++Y+AA
Sbjct:   21 MLELLASHYGTQ--LTETTEVAEAKNDIDSGTFDADAYTRDLLADKTLPDLIAYQETLAKKARSLDSSMQNLVYENYNKFIRATETIREMRDSVGELEAEMTTLRAAIDKTGATSAKLTGSLSSNRSKIEQLAGVRGLIGKLEFLFQLPARLRSCLELGAHQQAVKFYRAA 189
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BLAST of mRNA_M-pyrifera_M_contig83181.19429.1 vs. uniprot
Match: A0A5A8CFB0_CAFRO (Vacuolar protein sorting-associated protein 51 homolog n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CFB0_CAFRO)

HSP 1 Score: 157 bits (397), Expect = 2.500e-41
Identity = 80/171 (46.78%), Postives = 118/171 (69.01%), Query Frame = 1 
Query:    4 MRALLSSYYGVEDDADXXXXXXXARRDIDASGFDSAAHVSDVLRERPLPEVIAYHKRLSSEARGLDTGMQDLVYENYSKFLSATETVRVMRDRVGEIQAAMGRLEGRMALLGEASARVDGALKGNRERVEKLVAVRGLLDQLDFLFELPRRLQRAVALGAHSQAVRYYKAA 516
            M  LL+S+YG +           A+ DID+  FD+ A+  D+L ++ LP++IAY + L+ +AR LD+ MQ+LVYENY+KF+ ATET+R MRD VGE++A M  L   +   G  SA++ G+L  NR ++E+L  VRGL+ +L+FLF+LP RL+  + LGAH QAV++Y+AA
Sbjct:    8 MLELLASHYGTQ--LTETTEVAEAKNDIDSGTFDADAYTRDLLADKTLPDLIAYQETLAKKARSLDSSMQNLVYENYNKFIRATETIREMRDSVGELEAEMTTLRAAIDKTGATSAKLTGSLSSNRSKIEQLAGVRGLIGKLEFLFQLPARLRSCLELGAHQQAVKFYRAA 176
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BLAST of mRNA_M-pyrifera_M_contig83181.19429.1 vs. uniprot
Match: A0A5A8DPN1_CAFRO (Vacuolar protein sorting-associated protein 51 homolog n=2 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DPN1_CAFRO)

HSP 1 Score: 157 bits (397), Expect = 2.620e-41
Identity = 80/171 (46.78%), Postives = 118/171 (69.01%), Query Frame = 1 
Query:    4 MRALLSSYYGVEDDADXXXXXXXARRDIDASGFDSAAHVSDVLRERPLPEVIAYHKRLSSEARGLDTGMQDLVYENYSKFLSATETVRVMRDRVGEIQAAMGRLEGRMALLGEASARVDGALKGNRERVEKLVAVRGLLDQLDFLFELPRRLQRAVALGAHSQAVRYYKAA 516
            M  LL+S+YG +           A+ DID+  FD+ A+  D+L ++ LP++IAY + L+ +AR LD+ MQ+LVYENY+KF+ ATET+R MRD VGE++A M  L   +   G  SA++ G+L  NR ++E+L  VRGL+ +L+FLF+LP RL+  + LGAH QAV++Y+AA
Sbjct:    8 MLELLASHYGTQ--LTETTEVAEAKNDIDSGTFDADAYTRDLLADKTLPDLIAYQETLAKKARSLDSSMQNLVYENYNKFIRATETIREMRDSVGELEAEMTTLRAAIDKTGATSAKLTGSLSSNRSKIEQLAGVRGLIGKLEFLFQLPARLRSCLELGAHQQAVKFYRAA 176
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BLAST of mRNA_M-pyrifera_M_contig83181.19429.1 vs. uniprot
Match: D8MBB0_BLAHO (Vacuolar protein sorting-associated protein 51 homolog n=2 Tax=Blastocystis hominis TaxID=12968 RepID=D8MBB0_BLAHO)

HSP 1 Score: 135 bits (341), Expect = 8.990e-36
Identity = 72/169 (42.60%), Postives = 111/169 (65.68%), Query Frame = 1 
Query:    1 SMRALLSSYYGVEDDADXXXXXXXARRDIDASGFDSAAHVSDVLRERPLPEVIAYHKRLSSEARGLDTGMQDLVYENYSKFLSATETVRVMRDRVGEIQAAMGRLEGRMALLGEASARVDGALKGNRERVEKLVAVRGLLDQLDFLFELPRRLQRAVALGAHSQAVRYY 507
            ++RA+L  YYG ++  +          D+D+  FD   ++  +L+E+ LPE+I  + R+ SEA+ LD+ MQ LVYENYS+F+SA++TVR +++ VG+++  M  L  R+ ++   +  +   L GNR +V+KLV+VR LL +L FLF+LP RLQR V L A+ QAV YY
Sbjct:   19 NIRAMLQDYYGTDNGDEVKEDDYY---DMDSKVFDKDKYIPKILKEQSLPELIKTNVRVISEAKQLDSDMQKLVYENYSRFMSASKTVRTLQENVGDMETRMSSLRERIQVVSSVTDTIGSNLDGNRSKVDKLVSVRRLLKKLQFLFDLPLRLQRCVELEAYDQAVDYY 184
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BLAST of mRNA_M-pyrifera_M_contig83181.19429.1 vs. uniprot
Match: L1JC90_GUITC (Vacuolar protein sorting-associated protein 51 homolog n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1JC90_GUITC)

HSP 1 Score: 132 bits (331), Expect = 1.570e-32
Identity = 66/145 (45.52%), Postives = 104/145 (71.72%), Query Frame = 1 
Query:   82 DIDASGFDSAAHVSDVLRERPLPEVIAYHKRLSSEARGLDTGMQDLVYENYSKFLSATETVRVMRDRVGEIQAAMGRLEGRMALLGEASARVDGALKGNRERVEKLVAVRGLLDQLDFLFELPRRLQRAVALGAHSQAVRYYKAA 516
            DID+S FD+ A+VS +L+ +PLP+++     LSSE + LD+ MQ LVYENY+KF+SATET+R M+++V  +   M RL  +++ + E+S +++  L   R R+++L  +R LL +L F+FELP RL+RAV L A ++AV+Y++ A
Sbjct:   34 DIDSSNFDADAYVSSLLKSKPLPDLVGRSNELSSEIKELDSDMQMLVYENYNKFISATETIRAMKEKVDGMDGEMERLASKVSEITESSFKINSNLAERRVRIQRLNGIRRLLKKLSFIFELPTRLKRAVELDAGAEAVKYWRTA 178
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BLAST of mRNA_M-pyrifera_M_contig83181.19429.1 vs. uniprot
Match: A0A7S0M2X2_9CRYP (Vacuolar protein sorting-associated protein 51 homolog (Fragment) n=1 Tax=Cryptomonas curvata TaxID=233186 RepID=A0A7S0M2X2_9CRYP)

HSP 1 Score: 122 bits (306), Expect = 1.200e-31
Identity = 62/142 (43.66%), Postives = 98/142 (69.01%), Query Frame = 1 
Query:   82 DIDASGFDSAAHVSDVLRERPLPEVIAYHKRLSSEARGLDTGMQDLVYENYSKFLSATETVRVMRDRVGEIQAAMGRLEGRMALLGEASARVDGALKGNRERVEKLVAVRGLLDQLDFLFELPRRLQRAVALGAHSQAVRYY 507
            DID + FD+ A+VS +++ +PLPE++A    LS E + L++ MQ LVYENY+KF+SAT+T++ M+++V  ++  MG+L   +  +  AS  ++  L   R R++KL  VR LL +L F+F+LP RL RA+ L A ++AVRY+
Sbjct:   31 DIDGANFDATAYVSSLIKNKPLPELVARSNELSLEIKELESDMQMLVYENYNKFISATDTIQEMKNKVDSMEVEMGQLTTSIKEITTASLTINENLADRRVRIQKLNGVRRLLKKLSFIFDLPARLNRAIELDACTEAVRYW 172
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BLAST of mRNA_M-pyrifera_M_contig83181.19429.1 vs. uniprot
Match: A0A0P1A6A9_PLAHL (Vacuolar protein sorting-associated protein 51 homolog n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1A6A9_PLAHL)

HSP 1 Score: 128 bits (321), Expect = 4.100e-31
Identity = 70/168 (41.67%), Postives = 108/168 (64.29%), Query Frame = 1 
Query:    4 MRALLSSYYGVEDDADXXXXXXXARRDIDASGFDSAAHVSDVLRERPLPEVIAYHKRLSSEARGLDTGMQDLVYENYSKFLSATETVRVMRDRVGEIQAAMGRLEGRMALLGEASARVDGALKGNRERVEKLVAVRGLLDQLDFLFELPRRLQRAVALGAHSQAVRYY 507
            M+ LLSSYYG++D            R+ID+ GFDS  +V ++L+ R L ++++   +L  E + LDT MQ LVYENY+KF+SAT+T+R M+D V  ++A +GR+   M ++   S  ++ AL  +R  VEKL+ VR LL + +F+F+LP+RL  AV    ++ A +YY
Sbjct:   22 MQELLSSYYGLQDQESRQEQL----RNIDSPGFDSNLYVQELLKTRGLKDLLSTDDQLIREIKELDTNMQMLVYENYNKFISATDTIRKMKDNVASMEAEVGRVVKSMDIITAKSESINVALAPHRSTVEKLIGVRRLLKRFEFIFDLPQRLNTAVRQKNYANATKYY 185
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BLAST of mRNA_M-pyrifera_M_contig83181.19429.1 vs. uniprot
Match: A0A196S950_BLAHN (Vacuolar protein sorting-associated protein 51 homolog n=1 Tax=Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII) TaxID=478820 RepID=A0A196S950_BLAHN)

HSP 1 Score: 127 bits (319), Expect = 7.750e-31
Identity = 69/169 (40.83%), Postives = 108/169 (63.91%), Query Frame = 1 
Query:    1 SMRALLSSYYGVEDDADXXXXXXXARRDIDASGFDSAAHVSDVLRERPLPEVIAYHKRLSSEARGLDTGMQDLVYENYSKFLSATETVRVMRDRVGEIQAAMGRLEGRMALLGEASARVDGALKGNRERVEKLVAVRGLLDQLDFLFELPRRLQRAVALGAHSQAVRYY 507
            ++RA+L +YYG E   +          D+D+  FD   ++  VL+E+ LPE+I  + ++ +EA+ LD+ MQ LVYENYS+F++A++TVR ++  VG+++  M  L  R+  +   +  +  +L  NR +V+KLV V+ LL +L FLF+LP RLQR V L A+ QAV YY
Sbjct:   19 NIRAMLQNYYGTEQTEEVKEEDYY---DMDSKVFDKDKYIPKVLKEQSLPELIKTNIKVITEAKQLDSDMQKLVYENYSRFMTASKTVRTLQGNVGDMEKRMSSLMERIQTVSSTTNTIGSSLDANRTKVDKLVNVKRLLKKLQFLFDLPSRLQRCVELEAYDQAVDYY 184
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BLAST of mRNA_M-pyrifera_M_contig83181.19429.1 vs. uniprot
Match: UPI00071128CB (hypothetical protein n=1 Tax=Blastocystis sp. subtype 4 TaxID=944170 RepID=UPI00071128CB)

HSP 1 Score: 126 bits (317), Expect = 1.430e-30
Identity = 70/169 (41.42%), Postives = 108/169 (63.91%), Query Frame = 1 
Query:    1 SMRALLSSYYGVEDDADXXXXXXXARRDIDASGFDSAAHVSDVLRERPLPEVIAYHKRLSSEARGLDTGMQDLVYENYSKFLSATETVRVMRDRVGEIQAAMGRLEGRMALLGEASARVDGALKGNRERVEKLVAVRGLLDQLDFLFELPRRLQRAVALGAHSQAVRYY 507
            ++RA+L  YYG +   +          D+D+  FD    +  +L+E+ LP++I  + R+ SEA+ LD+ MQ LVYENYS+F+SA++TVR +++ VG+++  M  L  ++ ++   S  V   L  NR +V+KLV+V+ LL +L FLF+LP RLQR V L A+ QAV YY
Sbjct:   19 NIRAMLQDYYGTDTGDEVKEDDYY---DMDSKVFDKDKFIPKILKEQSLPDLIKTNIRVISEAKQLDSDMQKLVYENYSRFMSASKTVRTLQENVGDMETRMSSLLEKIQVVTTLSNTVGSNLDANRTKVDKLVSVKRLLKKLQFLFDLPSRLQRCVELEAYDQAVDYY 184
[Close]
BLAST of mRNA_M-pyrifera_M_contig83181.19429.1 vs. uniprot
Match: K3WGS7_GLOUD (Vacuolar protein sorting-associated protein 51 homolog n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WGS7_GLOUD)

HSP 1 Score: 126 bits (316), Expect = 1.940e-30
Identity = 68/168 (40.48%), Postives = 110/168 (65.48%), Query Frame = 1 
Query:    4 MRALLSSYYGVEDDADXXXXXXXARRDIDASGFDSAAHVSDVLRERPLPEVIAYHKRLSSEARGLDTGMQDLVYENYSKFLSATETVRVMRDRVGEIQAAMGRLEGRMALLGEASARVDGALKGNRERVEKLVAVRGLLDQLDFLFELPRRLQRAVALGAHSQAVRYY 507
            M+ LLSSYYG++D          + R+ID+ GFDS  +V ++L+ R L +++    +L  E + LDT MQ LVYENY+KF+SAT+T+R M++ V  ++  +G++   M ++ E S  ++ AL  +R +VEKL+ VR LL +L+F+F+LP+RL  A+    ++ A +YY
Sbjct:   10 MQELLSSYYGMQD----KESEAESLRNIDSKGFDSKIYVKEMLQMRGLNDLLQRDDQLIREIKELDTNMQMLVYENYNKFISATDTIRKMKNNVASMEEEVGKVVKSMDVITEKSESINVALAPHRSKVEKLIGVRRLLKRLEFIFDLPQRLNTALKQEEYANATKYY 173
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig83181.19429.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S0P836_CAFRO8.250e-4346.78Vacuolar protein sorting-associated protein 51 hom... [more]
A0A5A8CFB0_CAFRO2.500e-4146.78Vacuolar protein sorting-associated protein 51 hom... [more]
A0A5A8DPN1_CAFRO2.620e-4146.78Vacuolar protein sorting-associated protein 51 hom... [more]
D8MBB0_BLAHO8.990e-3642.60Vacuolar protein sorting-associated protein 51 hom... [more]
L1JC90_GUITC1.570e-3245.52Vacuolar protein sorting-associated protein 51 hom... [more]
A0A7S0M2X2_9CRYP1.200e-3143.66Vacuolar protein sorting-associated protein 51 hom... [more]
A0A0P1A6A9_PLAHL4.100e-3141.67Vacuolar protein sorting-associated protein 51 hom... [more]
A0A196S950_BLAHN7.750e-3140.83Vacuolar protein sorting-associated protein 51 hom... [more]
UPI00071128CB1.430e-3041.42hypothetical protein n=1 Tax=Blastocystis sp. subt... [more]
K3WGS7_GLOUD1.940e-3040.48Vacuolar protein sorting-associated protein 51 hom... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig83181contigM-pyrifera_M_contig83181:378..893 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score135.6
Seed ortholog evalue2.2e-29
Seed eggNOG ortholog55529.EKX46163
KEGG koko:K20296
Hectar predicted targeting categoryother localisation
GOsGO:0000938,GO:0003002,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005768,GO:0005794,GO:0006810,GO:0006914,GO:0006996,GO:0007030,GO:0007034,GO:0007041,GO:0007275,GO:0007389,GO:0008150,GO:0008152,GO:0009056,GO:0009987,GO:0010051,GO:0010256,GO:0010305,GO:0012505,GO:0016020,GO:0016021,GO:0016043,GO:0016192,GO:0016197,GO:0016482,GO:0031224,GO:0031410,GO:0031982,GO:0032456,GO:0032501,GO:0032502,GO:0032991,GO:0042147,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044248,GO:0044422,GO:0044424,GO:0044425,GO:0044431,GO:0044433,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0046907,GO:0048193,GO:0048856,GO:0051179,GO:0051234,GO:0051641,GO:0051649,GO:0055037,GO:0061919,GO:0071840,GO:0097708,GO:0099023,GO:1990745
EggNOG free text desc.endocytic recycling
EggNOG OGsKOG2346@1,KOG2346@2759
COG Functional cat.G
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04131
Exons1
Model size516
Cds size513
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816738.7083206-UTR-M-pyrifera_M_contig83181:377..3801622816738.7083206-UTR-M-pyrifera_M_contig83181:377..380Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig83181 378..380 +
1692278149.4354248-UTR-M-pyrifera_M_contig83181:377..3801692278149.4354248-UTR-M-pyrifera_M_contig83181:377..380Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig83181 378..380 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816738.7203572-CDS-M-pyrifera_M_contig83181:380..8931622816738.7203572-CDS-M-pyrifera_M_contig83181:380..893Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83181 381..893 +
1692278149.449132-CDS-M-pyrifera_M_contig83181:380..8931692278149.449132-CDS-M-pyrifera_M_contig83181:380..893Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83181 381..893 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig83181.19429.1prot_M-pyrifera_M_contig83181.19429.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig83181 381..893 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig83181.19429.1

>prot_M-pyrifera_M_contig83181.19429.1 ID=prot_M-pyrifera_M_contig83181.19429.1|Name=mRNA_M-pyrifera_M_contig83181.19429.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=171bp
MRALLSSYYGVEDDADAERERERARRDIDASGFDSAAHVSDVLRERPLPE
VIAYHKRLSSEARGLDTGMQDLVYENYSKFLSATETVRVMRDRVGEIQAA
MGRLEGRMALLGEASARVDGALKGNRERVEKLVAVRGLLDQLDFLFELPR
RLQRAVALGAHSQAVRYYKAA
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mRNA from alignment at M-pyrifera_M_contig83181:378..893+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig83181.19429.1 ID=mRNA_M-pyrifera_M_contig83181.19429.1|Name=mRNA_M-pyrifera_M_contig83181.19429.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=516bp|location=Sequence derived from alignment at M-pyrifera_M_contig83181:378..893+ (Macrocystis pyrifera P11B4 male)
AGCATGCGTGCGTTGTTGTCTTCTTACTATGGCGTGGAGGACGACGCCGA
CGCGGAGCGTGAGCGTGAGCGTGCCCGTCGGGACATTGACGCGAGTGGGT
TTGATAGCGCTGCGCATGTGAGCGACGTGTTGCGAGAGCGTCCGCTACCT
GAGGTGATTGCGTACCACAAGCGGCTGAGCTCAGAGGCGCGAGGGCTTGA
CACGGGGATGCAGGATTTGGTGTACGAGAACTACTCCAAGTTCTTGTCGG
CGACCGAGACTGTGCGCGTGATGCGAGATCGCGTCGGGGAGATCCAGGCG
GCGATGGGCCGGTTGGAGGGGAGGATGGCCTTGTTGGGGGAGGCGTCGGC
AAGAGTGGATGGGGCGTTGAAGGGCAACAGAGAGCGGGTCGAGAAACTGG
TGGCAGTGCGGGGTTTGTTGGATCAGCTCGACTTCCTCTTCGAACTGCCA
CGCAGACTGCAAAGAGCCGTTGCGCTTGGAGCACACTCGCAAGCAGTGCG
GTATTACAAGGCAGCG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig83181:378..893+

>mRNA_M-pyrifera_M_contig83181.19429.1 ID=mRNA_M-pyrifera_M_contig83181.19429.1|Name=mRNA_M-pyrifera_M_contig83181.19429.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1026bp|location=Sequence derived from alignment at M-pyrifera_M_contig83181:378..893+ (Macrocystis pyrifera P11B4 male)
ATGCGTGCGTTGTTGTCTTCTTACTATGGCGTGGAGGACGACGCCGACGC
GGAGCGTGAGCGTGAGCGTGCCCGTCGGGACATTGACGCGAGTGGGTTTG
ATAGCGCTGCGCATGTGAGCGACGTGTTGCGAGAGCGTCCGCTACCTGAG
GTGATTGCGTACCACAAGCGGCTGAGCTCAGAGGCGCGAGGGCTTGACAC
GGGGATGCAGGATTTGGTGTACGAGAACTACTCCAAGTTCTTGTCGGCGA
CCGAGACTGTGCGCGTGATGCGAGATCGCGTCGGGGAGATCCAGGCGGCG
ATGGGCCGGTTGGAGGGGAGGATGGCCTTGTTGGGGGAGGCGTCGGCAAG
AGTGGATGGGGCGTTGAAGGGCAACAGAGAGCGGGTCGAGAAACTGGTGG
CAGTGCGGGGTTTGTTGGATCAGCTCGACTTCCTCTTCGAACTGCCACGC
AGACTGCAAAGAGCCGTTGCGCTTGGAGCACACTCGCAAGCAGTGCGGTA
TTACAAGGCAGCGATGCGTGCGTTGTTGTCTTCTTACTATGGCGTGGAGG
ACGACGCCGACGCGGAGCGTGAGCGTGAGCGTGCCCGTCGGGACATTGAC
GCGAGTGGGTTTGATAGCGCTGCGCATGTGAGCGACGTGTTGCGAGAGCG
TCCGCTACCTGAGGTGATTGCGTACCACAAGCGGCTGAGCTCAGAGGCGC
GAGGGCTTGACACGGGGATGCAGGATTTGGTGTACGAGAACTACTCCAAG
TTCTTGTCGGCGACCGAGACTGTGCGCGTGATGCGAGATCGCGTCGGGGA
GATCCAGGCGGCGATGGGCCGGTTGGAGGGGAGGATGGCCTTGTTGGGGG
AGGCGTCGGCAAGAGTGGATGGGGCGTTGAAGGGCAACAGAGAGCGGGTC
GAGAAACTGGTGGCAGTGCGGGGTTTGTTGGATCAGCTCGACTTCCTCTT
CGAACTGCCACGCAGACTGCAAAGAGCCGTTGCGCTTGGAGCACACTCGC
AAGCAGTGCGGTATTACAAGGCAGCG
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