mRNA_M-pyrifera_M_contig82883.19368.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig82883.19368.1
Unique NamemRNA_M-pyrifera_M_contig82883.19368.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig82883.19368.1 vs. uniprot
Match: A0A7S1XDV1_9RHOD (Prohibitin n=1 Tax=Compsopogon caeruleus TaxID=31354 RepID=A0A7S1XDV1_9RHOD)

HSP 1 Score: 132 bits (331), Expect = 9.130e-36
Identity = 59/87 (67.82%), Postives = 78/87 (89.66%), Query Frame = 1 
Query:   10 GQRALIFSRLSGVKDEVYGEGTRVLLPHLEYPILFDVRTRPRVVQSLTGSRDLQMVQISLRVLTRPDPRHLPTIFKTIGEDYDEKVL 270
            G RA+IF+++SGV+DEVYGEGT V +P L+ P+++DVR +PRVVQSLTGSRDLQMVQ++LRVL+RPDP  LPTI++T+G D+DE+VL
Sbjct:   48 GHRAVIFNKISGVRDEVYGEGTHVRVPFLDVPVIYDVRAKPRVVQSLTGSRDLQMVQMTLRVLSRPDPNSLPTIYRTLGLDWDERVL 134
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BLAST of mRNA_M-pyrifera_M_contig82883.19368.1 vs. uniprot
Match: A0A5A8DCK9_CAFRO (Prohibitin n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DCK9_CAFRO)

HSP 1 Score: 128 bits (322), Expect = 1.320e-34
Identity = 62/90 (68.89%), Postives = 75/90 (83.33%), Query Frame = 1 
Query:    1 VKVGQRALIFSRLSGVKDEVYGEGTRVLLPHLEYPILFDVRTRPRVVQSLTGSRDLQMVQISLRVLTRPDPRHLPTIFKTIGEDYDEKVL 270
            V+ GQRA+IF+RL GV+D V GEGT +++P  + PI+FD+RTRPR VQSLTGSRDLQMVQISLRVLTRP    LP IF TIGED++EK+L
Sbjct:   37 VEGGQRAVIFNRLVGVRDVVVGEGTHLMIPWFDRPIIFDIRTRPRQVQSLTGSRDLQMVQISLRVLTRPQVERLPLIFSTIGEDWEEKIL 126
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BLAST of mRNA_M-pyrifera_M_contig82883.19368.1 vs. uniprot
Match: A0A813AKS3_9DINO (Prohibitin n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813AKS3_9DINO)

HSP 1 Score: 128 bits (322), Expect = 2.630e-34
Identity = 62/90 (68.89%), Postives = 75/90 (83.33%), Query Frame = 1 
Query:    1 VKVGQRALIFSRLSGVKDEVYGEGTRVLLPHLEYPILFDVRTRPRVVQSLTGSRDLQMVQISLRVLTRPDPRHLPTIFKTIGEDYDEKVL 270
            V+ GQRA+IF+RL GV+D V GEGT +++P  + PI+FD+RTRPR VQSLTGSRDLQMVQISLRVLTRP    LP IF TIGED++EK+L
Sbjct:   69 VEGGQRAVIFNRLVGVRDVVVGEGTHLMIPWFDRPIIFDIRTRPRQVQSLTGSRDLQMVQISLRVLTRPQVERLPLIFSTIGEDWEEKIL 158
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BLAST of mRNA_M-pyrifera_M_contig82883.19368.1 vs. uniprot
Match: A0A5J4YV96_PORPP (Prohibitin n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4YV96_PORPP)

HSP 1 Score: 126 bits (316), Expect = 1.080e-33
Identity = 56/90 (62.22%), Postives = 77/90 (85.56%), Query Frame = 1 
Query:    1 VKVGQRALIFSRLSGVKDEVYGEGTRVLLPHLEYPILFDVRTRPRVVQSLTGSRDLQMVQISLRVLTRPDPRHLPTIFKTIGEDYDEKVL 270
            V+ G RA+IF++++GV++EVYGEGT + LP ++ PI++DVR +PR++QSLTGSRDLQ VQISLRVL+RP P  LP I++T+G DYDE+VL
Sbjct:   39 VEGGHRAVIFNKITGVREEVYGEGTHLRLPFIDVPIIYDVRAKPRIIQSLTGSRDLQTVQISLRVLSRPVPEQLPAIYRTLGMDYDERVL 128
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BLAST of mRNA_M-pyrifera_M_contig82883.19368.1 vs. uniprot
Match: A0A7S3A6Y6_9RHOD (Prohibitin n=1 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S3A6Y6_9RHOD)

HSP 1 Score: 122 bits (307), Expect = 1.190e-33
Identity = 53/87 (60.92%), Postives = 76/87 (87.36%), Query Frame = 1 
Query:   10 GQRALIFSRLSGVKDEVYGEGTRVLLPHLEYPILFDVRTRPRVVQSLTGSRDLQMVQISLRVLTRPDPRHLPTIFKTIGEDYDEKVL 270
            G RA+IF++++GVK+ VYGEGT + +P ++ P+++DVR +PR +QSLTGSRDLQMVQI+LRVL+RP+P  LPTI++T+G D+DE+VL
Sbjct:   47 GHRAVIFNKITGVKENVYGEGTHLRVPFIDVPVIYDVRAKPRNIQSLTGSRDLQMVQITLRVLSRPNPTALPTIYRTLGPDWDERVL 133
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BLAST of mRNA_M-pyrifera_M_contig82883.19368.1 vs. uniprot
Match: UPI001CB8E4C3 (prohibitin-1, mitochondrial-like n=1 Tax=Erigeron canadensis TaxID=72917 RepID=UPI001CB8E4C3)

HSP 1 Score: 124 bits (312), Expect = 3.290e-33
Identity = 57/90 (63.33%), Postives = 76/90 (84.44%), Query Frame = 1 
Query:    1 VKVGQRALIFSRLSGVKDEVYGEGTRVLLPHLEYPILFDVRTRPRVVQSLTGSRDLQMVQISLRVLTRPDPRHLPTIFKTIGEDYDEKVL 270
            V+ G RA++F+RL GVKD+VY EGT VL+P LE PI++DVR RP +V+S +GSRDLQMV+I LRVL RP+P  LPTI++T+G+DY+E+VL
Sbjct:   35 VEGGHRAIVFNRLVGVKDKVYPEGTHVLIPWLERPIIYDVRARPHLVESTSGSRDLQMVKIGLRVLVRPEPDRLPTIYRTLGQDYNERVL 124
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BLAST of mRNA_M-pyrifera_M_contig82883.19368.1 vs. uniprot
Match: A0A7S3A954_9RHOD (Prohibitin n=5 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S3A954_9RHOD)

HSP 1 Score: 122 bits (307), Expect = 2.590e-32
Identity = 53/87 (60.92%), Postives = 76/87 (87.36%), Query Frame = 1 
Query:   10 GQRALIFSRLSGVKDEVYGEGTRVLLPHLEYPILFDVRTRPRVVQSLTGSRDLQMVQISLRVLTRPDPRHLPTIFKTIGEDYDEKVL 270
            G RA+IF++++GVK+ VYGEGT + +P ++ P+++DVR +PR +QSLTGSRDLQMVQI+LRVL+RP+P  LPTI++T+G D+DE+VL
Sbjct:   47 GHRAVIFNKITGVKENVYGEGTHLRVPFIDVPVIYDVRAKPRNIQSLTGSRDLQMVQITLRVLSRPNPTALPTIYRTLGPDWDERVL 133
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BLAST of mRNA_M-pyrifera_M_contig82883.19368.1 vs. uniprot
Match: A0A453MG48_AEGTS (Prohibitin n=2 Tax=Triticinae TaxID=1648030 RepID=A0A453MG48_AEGTS)

HSP 1 Score: 120 bits (302), Expect = 2.730e-32
Identity = 53/90 (58.89%), Postives = 75/90 (83.33%), Query Frame = 1 
Query:    1 VKVGQRALIFSRLSGVKDEVYGEGTRVLLPHLEYPILFDVRTRPRVVQSLTGSRDLQMVQISLRVLTRPDPRHLPTIFKTIGEDYDEKVL 270
            V+ G RA++F+RL G+KD+VY EGT +++P  E PI++DVR RP +V+S +GSRDLQMV+I LRVLTRP P  LPT+++T+GE+Y+E+VL
Sbjct:   39 VEGGHRAIVFNRLEGIKDKVYPEGTHIVIPWFERPIIYDVRARPNLVESTSGSRDLQMVRIGLRVLTRPMPERLPTMYRTLGENYNERVL 128
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BLAST of mRNA_M-pyrifera_M_contig82883.19368.1 vs. uniprot
Match: M2XRM2_GALSU (Prohibitin n=1 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2XRM2_GALSU)

HSP 1 Score: 122 bits (307), Expect = 3.200e-32
Identity = 55/87 (63.22%), Postives = 73/87 (83.91%), Query Frame = 1 
Query:   10 GQRALIFSRLSGVKDEVYGEGTRVLLPHLEYPILFDVRTRPRVVQSLTGSRDLQMVQISLRVLTRPDPRHLPTIFKTIGEDYDEKVL 270
            G RA+IF++ +GV+ +VYGEGT V +P  + PI++DVR +PR VQSLTGSRDLQMVQI+LRVL+RPDP  +P I++T+G DYDE+VL
Sbjct:   47 GHRAVIFNKFTGVRPKVYGEGTHVRIPFFDVPIIYDVRAKPRSVQSLTGSRDLQMVQITLRVLSRPDPDKIPVIYQTLGTDYDERVL 133
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BLAST of mRNA_M-pyrifera_M_contig82883.19368.1 vs. uniprot
Match: A0A0K9R2L0_SPIOL (Prohibitin n=2 Tax=Spinacia oleracea TaxID=3562 RepID=A0A0K9R2L0_SPIOL)

HSP 1 Score: 122 bits (306), Expect = 3.500e-32
Identity = 55/90 (61.11%), Postives = 76/90 (84.44%), Query Frame = 1 
Query:    1 VKVGQRALIFSRLSGVKDEVYGEGTRVLLPHLEYPILFDVRTRPRVVQSLTGSRDLQMVQISLRVLTRPDPRHLPTIFKTIGEDYDEKVL 270
            V+ G RA++F+R+SG+KD+VY EGT +++P LE PI++DVR RP +V+S +GSRDLQMV+I LRVLTRP P  LPTI++T+GE+Y E+VL
Sbjct:   45 VEGGHRAIVFNRISGIKDKVYPEGTHLMIPWLERPIIYDVRARPHLVESTSGSRDLQMVKIGLRVLTRPLPETLPTIYRTLGENYSERVL 134
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig82883.19368.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S1XDV1_9RHOD9.130e-3667.82Prohibitin n=1 Tax=Compsopogon caeruleus TaxID=313... [more]
A0A5A8DCK9_CAFRO1.320e-3468.89Prohibitin n=1 Tax=Cafeteria roenbergensis TaxID=3... [more]
A0A813AKS3_9DINO2.630e-3468.89Prohibitin n=1 Tax=Symbiodinium sp. KB8 TaxID=2309... [more]
A0A5J4YV96_PORPP1.080e-3362.22Prohibitin n=1 Tax=Porphyridium purpureum TaxID=35... [more]
A0A7S3A6Y6_9RHOD1.190e-3360.92Prohibitin n=1 Tax=Rhodosorus marinus TaxID=101924... [more]
UPI001CB8E4C33.290e-3363.33prohibitin-1, mitochondrial-like n=1 Tax=Erigeron ... [more]
A0A7S3A954_9RHOD2.590e-3260.92Prohibitin n=5 Tax=Rhodosorus marinus TaxID=101924... [more]
A0A453MG48_AEGTS2.730e-3258.89Prohibitin n=2 Tax=Triticinae TaxID=1648030 RepID=... [more]
M2XRM2_GALSU3.200e-3263.22Prohibitin n=1 Tax=Galdieria sulphuraria TaxID=130... [more]
A0A0K9R2L0_SPIOL3.500e-3261.11Prohibitin n=2 Tax=Spinacia oleracea TaxID=3562 Re... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig82883contigM-pyrifera_M_contig82883:636..905 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score122.9
Seed ortholog evalue7.8e-26
Seed eggNOG ortholog130081.XP_005709402.1
Preferred namePHB2
KEGG koko:K17081
Hectar predicted targeting categoryother localisation
GOsGO:0000001,GO:0000003,GO:0000060,GO:0000819,GO:0001101,GO:0001302,GO:0001666,GO:0001763,GO:0001932,GO:0001934,GO:0002009,GO:0002082,GO:0003006,GO:0003008,GO:0003674,GO:0005102,GO:0005488,GO:0005515,GO:0005575,GO:0005618,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0005740,GO:0005741,GO:0005743,GO:0005746,GO:0005747,GO:0005773,GO:0005774,GO:0005911,GO:0006140,GO:0006355,GO:0006457,GO:0006606,GO:0006810,GO:0006811,GO:0006812,GO:0006816,GO:0006851,GO:0006886,GO:0006913,GO:0006950,GO:0006979,GO:0006996,GO:0007005,GO:0007049,GO:0007059,GO:0007062,GO:0007088,GO:0007096,GO:0007275,GO:0007276,GO:0007281,GO:0007283,GO:0007292,GO:0007346,GO:0007548,GO:0007568,GO:0007569,GO:0007588,GO:0008022,GO:0008104,GO:0008134,GO:0008150,GO:0008285,GO:0008406,GO:0009506,GO:0009507,GO:0009536,GO:0009611,GO:0009628,GO:0009653,GO:0009790,GO:0009792,GO:0009887,GO:0009888,GO:0009889,GO:0009890,GO:0009892,GO:0009893,GO:0009966,GO:0009967,GO:0009968,GO:0009986,GO:0009987,GO:0010033,GO:0010468,GO:0010556,GO:0010558,GO:0010562,GO:0010564,GO:0010604,GO:0010605,GO:0010629,GO:0010638,GO:0010646,GO:0010647,GO:0010648,GO:0010941,GO:0015031,GO:0015833,GO:0016020,GO:0016043,GO:0016363,GO:0017038,GO:0019219,GO:0019220,GO:0019222,GO:0019866,GO:0019867,GO:0019953,GO:0022402,GO:0022412,GO:0022414,GO:0022603,GO:0022612,GO:0023051,GO:0023056,GO:0023057,GO:0030001,GO:0030054,GO:0030154,GO:0030162,GO:0030312,GO:0030331,GO:0030421,GO:0030879,GO:0030964,GO:0031090,GO:0031323,GO:0031324,GO:0031325,GO:0031326,GO:0031327,GO:0031399,GO:0031401,GO:0031536,GO:0031647,GO:0031966,GO:0031967,GO:0031968,GO:0031974,GO:0031975,GO:0031981,GO:0032268,GO:0032269,GO:0032270,GO:0032501,GO:0032502,GO:0032504,GO:0032526,GO:0032989,GO:0032990,GO:0032991,GO:0033036,GO:0033043,GO:0033143,GO:0033144,GO:0033146,GO:0033147,GO:0033218,GO:0033365,GO:0033554,GO:0033599,GO:0033600,GO:0033993,GO:0034220,GO:0034399,GO:0034504,GO:0034613,GO:0035239,GO:0035257,GO:0035258,GO:0035295,GO:0035632,GO:0036293,GO:0036294,GO:0040008,GO:0040014,GO:0040018,GO:0042127,GO:0042221,GO:0042325,GO:0042327,GO:0042695,GO:0042886,GO:0042981,GO:0043051,GO:0043066,GO:0043067,GO:0043069,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043408,GO:0043410,GO:0043433,GO:0043467,GO:0044092,GO:0044093,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044429,GO:0044437,GO:0044444,GO:0044446,GO:0044455,GO:0044464,GO:0044703,GO:0045136,GO:0045137,GO:0045184,GO:0045271,GO:0045787,GO:0045840,GO:0045861,GO:0045892,GO:0045927,GO:0045931,GO:0045934,GO:0045937,GO:0046543,GO:0046907,GO:0047485,GO:0048232,GO:0048308,GO:0048311,GO:0048468,GO:0048477,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0048585,GO:0048608,GO:0048609,GO:0048638,GO:0048639,GO:0048729,GO:0048731,GO:0048732,GO:0048754,GO:0048856,GO:0048869,GO:0050678,GO:0050680,GO:0050789,GO:0050793,GO:0050794,GO:0050795,GO:0050821,GO:0050896,GO:0051090,GO:0051091,GO:0051093,GO:0051094,GO:0051128,GO:0051130,GO:0051169,GO:0051170,GO:0051171,GO:0051172,GO:0051173,GO:0051174,GO:0051179,GO:0051234,GO:0051239,GO:0051240,GO:0051241,GO:0051246,GO:0051247,GO:0051248,GO:0051252,GO:0051253,GO:0051276,GO:0051427,GO:0051640,GO:0051641,GO:0051646,GO:0051649,GO:0051704,GO:0051716,GO:0051726,GO:0051783,GO:0051785,GO:0055044,GO:0055085,GO:0060255,GO:0060259,GO:0060429,GO:0060443,GO:0060444,GO:0060548,GO:0060562,GO:0060603,GO:0060688,GO:0060744,GO:0060749,GO:0060762,GO:0061138,GO:0061180,GO:0061377,GO:0061458,GO:0062012,GO:0065007,GO:0065008,GO:0065009,GO:0070013,GO:0070372,GO:0070374,GO:0070469,GO:0070482,GO:0070584,GO:0070588,GO:0070727,GO:0070838,GO:0070887,GO:0071229,GO:0071300,GO:0071310,GO:0071396,GO:0071453,GO:0071456,GO:0071702,GO:0071705,GO:0071840,GO:0071944,GO:0072511,GO:0072594,GO:0080090,GO:0090068,GO:0098588,GO:0098655,GO:0098660,GO:0098662,GO:0098796,GO:0098798,GO:0098800,GO:0098803,GO:0098805,GO:0098813,GO:1900542,GO:1901700,GO:1901701,GO:1901987,GO:1901989,GO:1901990,GO:1901992,GO:1902494,GO:1902531,GO:1902533,GO:1902679,GO:1902806,GO:1902808,GO:1903506,GO:1903507,GO:1903578,GO:1903998,GO:1905330,GO:1990204,GO:2000026,GO:2000027,GO:2000112,GO:2000113,GO:2001141
EggNOG free text desc.thelarche
EggNOG OGsCOG0330@1,KOG3090@2759
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03029,ko04147
Exons1
Model size270
Cds size270
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816735.7426069-CDS-M-pyrifera_M_contig82883:635..9051622816735.7426069-CDS-M-pyrifera_M_contig82883:635..905Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82883 636..905 +
1692278147.7380216-CDS-M-pyrifera_M_contig82883:635..9051692278147.7380216-CDS-M-pyrifera_M_contig82883:635..905Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82883 636..905 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig82883.19368.1prot_M-pyrifera_M_contig82883.19368.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig82883 636..905 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig82883.19368.1

>prot_M-pyrifera_M_contig82883.19368.1 ID=prot_M-pyrifera_M_contig82883.19368.1|Name=mRNA_M-pyrifera_M_contig82883.19368.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=90bp
VKVGQRALIFSRLSGVKDEVYGEGTRVLLPHLEYPILFDVRTRPRVVQSL
TGSRDLQMVQISLRVLTRPDPRHLPTIFKTIGEDYDEKVL
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mRNA from alignment at M-pyrifera_M_contig82883:636..905+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig82883.19368.1 ID=mRNA_M-pyrifera_M_contig82883.19368.1|Name=mRNA_M-pyrifera_M_contig82883.19368.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=270bp|location=Sequence derived from alignment at M-pyrifera_M_contig82883:636..905+ (Macrocystis pyrifera P11B4 male)
GTCAAGGTTGGTCAGCGGGCTTTGATTTTCTCTCGTCTGAGCGGTGTGAA
GGACGAGGTGTACGGCGAGGGGACTCGGGTGTTGCTTCCGCACCTGGAGT
ATCCGATTCTGTTCGATGTTCGGACTCGTCCTCGGGTGGTGCAGAGCCTG
ACTGGGTCTCGCGATTTGCAGATGGTTCAGATTTCGCTGCGTGTCTTGAC
TCGGCCTGATCCCCGGCACTTGCCCACGATTTTCAAGACCATTGGGGAGG
ACTACGACGAGAAGGTGCTG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig82883:636..905+

>mRNA_M-pyrifera_M_contig82883.19368.1 ID=mRNA_M-pyrifera_M_contig82883.19368.1|Name=mRNA_M-pyrifera_M_contig82883.19368.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=540bp|location=Sequence derived from alignment at M-pyrifera_M_contig82883:636..905+ (Macrocystis pyrifera P11B4 male)
GTCAAGGTTGGTCAGCGGGCTTTGATTTTCTCTCGTCTGAGCGGTGTGAA
GGACGAGGTGTACGGCGAGGGGACTCGGGTGTTGCTTCCGCACCTGGAGT
ATCCGATTCTGTTCGATGTTCGGACTCGTCCTCGGGTGGTGCAGAGCCTG
ACTGGGTCTCGCGATTTGCAGATGGTTCAGATTTCGCTGCGTGTCTTGAC
TCGGCCTGATCCCCGGCACTTGCCCACGATTTTCAAGACCATTGGGGAGG
ACTACGACGAGAAGGTGCTGGTCAAGGTTGGTCAGCGGGCTTTGATTTTC
TCTCGTCTGAGCGGTGTGAAGGACGAGGTGTACGGCGAGGGGACTCGGGT
GTTGCTTCCGCACCTGGAGTATCCGATTCTGTTCGATGTTCGGACTCGTC
CTCGGGTGGTGCAGAGCCTGACTGGGTCTCGCGATTTGCAGATGGTTCAG
ATTTCGCTGCGTGTCTTGACTCGGCCTGATCCCCGGCACTTGCCCACGAT
TTTCAAGACCATTGGGGAGGACTACGACGAGAAGGTGCTG
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