mRNA_M-pyrifera_M_contig82242.19234.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig82242.19234.1
Unique NamemRNA_M-pyrifera_M_contig82242.19234.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig82242.19234.1 vs. uniprot
Match: A0A0P1B581_PLAHL (Cobalamin synthesis protein n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1B581_PLAHL)

HSP 1 Score: 134 bits (338), Expect = 3.050e-34
Identity = 79/189 (41.80%), Postives = 115/189 (60.85%), Query Frame = 1
Query:    1 LNKLLRAAGVAGKRIGVIENEAGPISIDHRLLRDAAMDPDGIFVLENGCICCTTGGS----GDEVERVLDRAVSMMNASGEGLDHLVVETSGLADPGPLLELLFLPHRAGALHLDGVVTLVDAKHGVQHLVESGGWGGRAVEASRQVAYADEVLVTKVDL-SPDTVGPLHKAIETLNPTTQIRNVVLVD 552
            LN++L        +I V+ENE G +SIDH LL+    + DGI+VL+NGC+CCT  G     GDE+ER+LD  + ++    +  D+LVVETSGLADPGP++E  FL  RA    LD +VT+VD+K   +H  +S       +E  RQ+ YAD V++ K+DL S +    L KA++ +N   ++ + V  D
Sbjct:  105 LNRILLTEDQLTYKIMVLENELGAVSIDHTLLQAGGKEEDGIYVLQNGCMCCTARGGKGKGGDELERILDYLLQLVEK--QKFDYLVVETSGLADPGPIIET-FLRLRASRFRLDAIVTMVDSKATQRHWKQSKEEYDFPIELHRQILYADIVVMNKLDLASAEECKRLEKALKKINEEARVCSCVNAD 290          
BLAST of mRNA_M-pyrifera_M_contig82242.19234.1 vs. uniprot
Match: H3H330_PHYRM (CobW C-terminal domain-containing protein n=1 Tax=Phytophthora ramorum TaxID=164328 RepID=H3H330_PHYRM)

HSP 1 Score: 133 bits (335), Expect = 9.170e-34
Identity = 78/186 (41.94%), Postives = 112/186 (60.22%), Query Frame = 1
Query:    1 LNKLLRAAGVAGKRIGVIENEAGPISIDHRLLRDAAMDPDGIFVLENGCICCTTGGS----GDEVERVLDRAVSMMNASGEGLDHLVVETSGLADPGPLLELLFLPHRAGALHLDGVVTLVDAKHGVQHLVESGGWGGRAVEASRQVAYADEVLVTKVDLSP-DTVGPLHKAIETLNPTTQIRNVV 543
            LN++L        +I V+ENE G +SIDHRLL+    + DGI+VL+NGC+CCT  G     GDE+ER+LD  + ++    +  D+LVVET+GLADPGP++E  FL  RA    LD +VT+VDAK   ++  E  G+    +E  RQ  YAD V++ KVD++  D    L  A+  +N    + + V
Sbjct:  108 LNRILLTEDQLPYKIMVLENELGAVSIDHRLLQAGGKEMDGIYVLQNGCMCCTARGGKGKGGDELERILDYLLQLVQE--QSFDYLVVETTGLADPGPIIET-FLRLRASRFRLDAIVTMVDAKATQRYWKEEKGFYEFPIELQRQALYADIVVLNKVDVATKDECKRLEDALTGINEEASVCSCV 290          
BLAST of mRNA_M-pyrifera_M_contig82242.19234.1 vs. uniprot
Match: L1JC45_GUITC (Uncharacterized protein n=1 Tax=Guillardia theta (strain CCMP2712) TaxID=905079 RepID=L1JC45_GUITC)

HSP 1 Score: 129 bits (325), Expect = 1.180e-32
Identity = 72/172 (41.86%), Postives = 109/172 (63.37%), Query Frame = 1
Query:   34 GKRIGVIENEAGPISIDHRLLRDAAMDPD--GIFVLENGCICCTTGGSGDEVERVLDRAVSMMNASGEGLDHLVVETSGLADPGPLLELLF-LPHRAGALHLDGVVTLVDAKHGVQHLVESGGWGGRAVEASRQVAYADEVLVTKVDLSPD---TVGPLHKAIETLNPTTQI 531
            GKRI VIENEAG ISID ++LR    +    GIFV++NGC+CCT  G+G E+ERVL+  ++++    +  D++++ETSGLADP P+++  F L + +G   LDGVV ++D  +  QHL +  GWG +  E  RQ+A+A  +L+ K D   D    +  L + +  LNP  ++
Sbjct:   80 GKRIVVIENEAGSISIDDKVLRAGEQEKAAVGIFVMKNGCMCCTGDGAGPELERVLNHLLNILQD--DEYDYVLIETSGLADPAPIMQTFFSLSNASGRFFLDGVVAIIDCHNVWQHL-DGRGWGAKTPEVYRQIAHATSILMNKTDRRNDQEENLKELEELLGELNPHAKV 248          
BLAST of mRNA_M-pyrifera_M_contig82242.19234.1 vs. uniprot
Match: A0A7S3SFJ0_9SPIT (Hypothetical protein (Fragment) n=1 Tax=Strombidinopsis acuminata TaxID=141414 RepID=A0A7S3SFJ0_9SPIT)

HSP 1 Score: 125 bits (315), Expect = 4.220e-32
Identity = 79/191 (41.36%), Postives = 115/191 (60.21%), Query Frame = 1
Query:   25 GVAGKRIGVIENEAGPISIDHRLLRDAAMD--PDGIFVLENGCICCTTGGSGDEVERVLDRAVSM--------MNASGEG-----------LDHLVVETSGLADPGPLLELLFLPHRAGA-LHLDGVVTLVDAKHGVQHLVESGGWG--GRAVEASRQVAYADEVLVTKVDL---SPDTVGPLHKAIETLN 516
            G  G+RI VIENE G ISIDH L+ +A     P G+ VL+NGC+CC+    G E+ERVLD+ + +          +S EG            D++++ETSGLADPGP++++L+    A +  +LDGVVT+VDA H ++HL  SG +    R  EA +QVA AD +++ KVDL   +PD +  + + +  LN
Sbjct:   11 GDHGRRILVIENELGAISIDHELIDEARQQEAPSGVIVLKNGCMCCSGETPGSELERVLDQLLQIGKVDARTSETSSSEGAGDSPAKASLPFDYVLIETSGLADPGPVMQVLWRQEMARSPFYLDGVVTVVDAAHILRHLRPSGPFAFTRRRPEAEKQVAVADRLVINKVDLVESTPDELTAVSEMLSELN 201          
BLAST of mRNA_M-pyrifera_M_contig82242.19234.1 vs. uniprot
Match: A0A6A4F463_9STRA (CobW C-terminal domain-containing protein n=7 Tax=Phytophthora TaxID=4783 RepID=A0A6A4F463_9STRA)

HSP 1 Score: 129 bits (323), Expect = 5.670e-32
Identity = 76/177 (42.94%), Postives = 107/177 (60.45%), Query Frame = 1
Query:    1 LNKLLRAAGVAGKRIGVIENEAGPISIDHRLLRDAAMDPDGIFVLENGCICCTTGGS----GDEVERVLDRAVSMMNASGEGLDHLVVETSGLADPGPLLELLFLPHRAGALHLDGVVTLVDAKHGVQHLVESGGWGGRAVEASRQVAYADEVLVTKVDLSP-DTVGPLHKAIETLN 516
            LNK+L        +I V+ENE G +SIDH LL+    + DGI+VL+NGC+CCT  G     GDE+ER+LD  + ++    +  D+LVVET+GLADPGP++E  FL  RA    LD +VT+VDAK   ++  +        +E  RQ  YAD V++ KVDL+  +    L KA+  +N
Sbjct:  119 LNKILLTEDSLPYKIMVLENELGAVSIDHALLQAGGKEVDGIYVLQNGCMCCTARGGKGRGGDELERILDYLLQLVQE--QSFDYLVVETTGLADPGPIIET-FLRLRASRFRLDAIVTMVDAKATQRYWKQEKDAYEFPIELQRQALYADIVVMNKVDLATSEECERLEKALAVIN 292          
BLAST of mRNA_M-pyrifera_M_contig82242.19234.1 vs. uniprot
Match: A0A225VF61_9STRA (Cobalamin synthesis protein n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225VF61_9STRA)

HSP 1 Score: 128 bits (322), Expect = 5.820e-32
Identity = 78/186 (41.94%), Postives = 112/186 (60.22%), Query Frame = 1
Query:    1 LNKLLRAAGVAGKRIGVIENEAGPISIDHRLLRDAAMDPDGIFVLENGCICCTTGGS----GDEVERVLDRAVSMMNASGEGLDHLVVETSGLADPGPLLELLFLPHRAGALHLDGVVTLVDAKHGVQHLVESGGWGGRAVEASRQVAYADEVLVTKVDLSP-DTVGPLHKAIETLNPTTQIRNVV 543
            LN++L        +I V+ENE G +SIDH LL+    + DGI+VL+NGC+CCT  G     GDE+ER+LD  + ++    +  D+LVVET+GLADPGP++E  FL  RA    LD +VT+VDAK    +  ES  +    +E  RQV YAD V++ KVD++  +    L KA+  +N    + + V
Sbjct:  100 LNRILLTEDQLPYKIMVLENELGAVSIDHTLLQAGGKEKDGIYVLKNGCMCCTARGGKGKGGDELERILDYLLQLVEE--QSFDYLVVETTGLADPGPIIET-FLRLRASRFRLDAIVTMVDAKATQTYWKESYQF---PIELQRQVLYADIVVMNKVDVATKEECKRLEKALTEINEEATVCSCV 279          
BLAST of mRNA_M-pyrifera_M_contig82242.19234.1 vs. uniprot
Match: A0A7S4AZJ1_CHRCT (Hypothetical protein n=1 Tax=Chrysotila carterae TaxID=13221 RepID=A0A7S4AZJ1_CHRCT)

HSP 1 Score: 127 bits (319), Expect = 1.080e-31
Identity = 74/163 (45.40%), Postives = 104/163 (63.80%), Query Frame = 1
Query:    1 LNKLLRAAGVAGKRIGVIENEAGPISIDHRLLRDAAMD--PDGIFVLENGCICCTTGGSGDEVERVLDRAVSMMNASGEGL--DHLVVETSGLADPGPLLELLFLPHRAGA-LHLDGVVTLVDAKHGVQHLVESG--GWGGRAVEASRQVAYADEVLVTKVDL 468
            +N++L  AG  G+RI VIENE G ISIDH L+ + A    P G+ VL+NGC+CCT    G E+ERVLD+ +         L  D +V+ET+G+ADP P++++L      GA   LDGVVT+VDAK+ ++H+   G  G+     EA +Q+A AD +L+ KVDL
Sbjct:   84 VNRIL--AGDHGQRILVIENELGAISIDHELIDEQAQKNAPSGVIVLKNGCMCCTGESPGSELERVLDKLLDFSQGDSASLVFDRIVIETTGMADPSPIIQVLLRSEMNGARFRLDGVVTVVDAKNLMRHISPQGPAGFLRHRPEAEKQIAMADLLLINKVDL 244          
BLAST of mRNA_M-pyrifera_M_contig82242.19234.1 vs. uniprot
Match: W2PPC4_PHYPN (CobW C-terminal domain-containing protein n=11 Tax=Phytophthora TaxID=4783 RepID=W2PPC4_PHYPN)

HSP 1 Score: 127 bits (319), Expect = 1.800e-31
Identity = 75/189 (39.68%), Postives = 112/189 (59.26%), Query Frame = 1
Query:    1 LNKLLRAAGVAGKRIGVIENEAGPISIDHRLLRDAAMDPDGIFVLENGCICCTTGGS----GDEVERVLDRAVSMMNASGEGLDHLVVETSGLADPGPLLELLFLPHRAGALHLDGVVTLVDAKHGVQHLVESGGWGGRAVEASRQVAYADEVLVTKVDLSP-DTVGPLHKAIETLNPTTQIRNVVLVD 552
            LN++L        +I V+ENE G +SIDH LL     + DGI+VL+NGC+CCT  G     GDE+ER+LD  + ++    +  D+L+VET+GLADPGP++E  FL  RA    LD +VT+VDAK   ++  ++  +    +E  RQ  YAD +++ KVD++  D    L KA+  +N    + + V  D
Sbjct:  108 LNRILLTEDKLPYKIMVLENELGAVSIDHTLLEAGGKEKDGIYVLQNGCMCCTARGGKGKGGDELERILDYLLQLVQE--QSFDYLIVETTGLADPGPIIET-FLRLRASRFRLDAIVTMVDAKATQRYWKQNERYEF-PIELQRQALYADIIVMNKVDVATKDECKRLEKALTEINEEATVCSCVKAD 292          
BLAST of mRNA_M-pyrifera_M_contig82242.19234.1 vs. uniprot
Match: A0A662Y6W2_9STRA (CobW C-terminal domain-containing protein n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662Y6W2_9STRA)

HSP 1 Score: 126 bits (317), Expect = 3.580e-31
Identity = 78/190 (41.05%), Postives = 113/190 (59.47%), Query Frame = 1
Query:    1 LNKLLRAAGVAGKRIGVIENEAGPISIDHRLLRDAAMDPDGIFVLENGCICCT----TGGSGDEVERVLDRAVSMMNASGEGLDHLVVETSGLADPGPLLELLFLPHRAGALHLDGVVTLVDAKHGVQHLVESGGWGGRA----VEASRQVAYADEVLVTKVDLSP-DTVGPLHKAIETLNPTTQIRNVV 543
            LN++L    +  K I V+ENE G +SIDH LL+  + + DGI+VL+NGC+CCT     G  GDE+ER+LD  + ++N   +  D+LVVET+GLADPGP++E  FL  RA    LD +V +VDAK    +      W G +    +E  RQ  YAD V++ KVD++  +    L  A+E +N    + + V
Sbjct:  109 LNRILHTEELPYK-IMVLENELGAVSIDHALLQSGSEERDGIYVLKNGCMCCTGRGGKGKGGDELERILDYLLRLVNE--QSFDYLVVETTGLADPGPIIET-FLRLRASRFRLDAIVAMVDAKAAQTY------WKGDSYVFPIELQRQALYADIVVLNKVDMATSEEQSRLEHALEAINEEATVCSCV 288          
BLAST of mRNA_M-pyrifera_M_contig82242.19234.1 vs. uniprot
Match: A0A5A8DII6_CAFRO (Uncharacterized protein n=5 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DII6_CAFRO)

HSP 1 Score: 125 bits (314), Expect = 1.460e-30
Identity = 88/217 (40.55%), Postives = 115/217 (53.00%), Query Frame = 1
Query:    1 LNKLLRAAGVAGKRIGVIENEAGPISIDHRLLRDAAMD-PDGIFVLENGCICCT--TGGSGDEVERVLDRAVSMMNASGEG----LDHLVVETSGLADPGPLLELLFLPHRAG--------ALHLDGVVTLVDAKHGVQHLVE-----------------SGGW---GGRAVEASRQVAYADEVLVTKVDL-SPDTVGPLHKAIETLNPTTQIRNVV 543
            LN LLR       RI VIENE G + +DH LLR A       +FVL NGC+CC+  +GG+G E+ERVLD  + +  A+ E     +DH+V+ETSGLADP PL+EL F    A         A  L GVVT+ DAK+ ++HL                   S  W    G AVEA RQVA+AD + + KVDL   +      +AI  +NP  ++   V
Sbjct:   87 LNALLRCKD---SRICVIENEVGSVGLDHDLLRGATEGFAKDVFVLANGCVCCSAGSGGTGGELERVLDLVLDIALATREAGEPPVDHVVIETSGLADPAPLVELFFTAGGADDSSASPSRAFELRGVVTVADAKNILRHLPGDVAAAAGRRTDPADSELSSAWLAGSGAAVEAGRQVAFADRIWLNKVDLVGAEAAAEAERAIARVNPMAELTRCV 300          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig82242.19234.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0P1B581_PLAHL3.050e-3441.80Cobalamin synthesis protein n=1 Tax=Plasmopara hal... [more]
H3H330_PHYRM9.170e-3441.94CobW C-terminal domain-containing protein n=1 Tax=... [more]
L1JC45_GUITC1.180e-3241.86Uncharacterized protein n=1 Tax=Guillardia theta (... [more]
A0A7S3SFJ0_9SPIT4.220e-3241.36Hypothetical protein (Fragment) n=1 Tax=Strombidin... [more]
A0A6A4F463_9STRA5.670e-3242.94CobW C-terminal domain-containing protein n=7 Tax=... [more]
A0A225VF61_9STRA5.820e-3241.94Cobalamin synthesis protein n=1 Tax=Phytophthora m... [more]
A0A7S4AZJ1_CHRCT1.080e-3145.40Hypothetical protein n=1 Tax=Chrysotila carterae T... [more]
W2PPC4_PHYPN1.800e-3139.68CobW C-terminal domain-containing protein n=11 Tax... [more]
A0A662Y6W2_9STRA3.580e-3141.05CobW C-terminal domain-containing protein n=2 Tax=... [more]
A0A5A8DII6_CAFRO1.460e-3040.55Uncharacterized protein n=5 Tax=Cafeteria roenberg... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig82242contigM-pyrifera_M_contig82242:2..553 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score136.7
Seed ortholog evalue1.1e-29
Seed eggNOG ortholog164328.Phyra84863
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Cobalamin synthesis protein cobW C-terminal domain
EggNOG OGs3QEKA@4776,COG0523@1,KOG2743@2759
COG Functional cat.H
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
Exons1
Model size552
Cds size552
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816729.424964-CDS-M-pyrifera_M_contig82242:1..5531622816729.424964-CDS-M-pyrifera_M_contig82242:1..553Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82242 2..553 +
1692278144.4660516-CDS-M-pyrifera_M_contig82242:1..5531692278144.4660516-CDS-M-pyrifera_M_contig82242:1..553Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82242 2..553 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig82242.19234.1prot_M-pyrifera_M_contig82242.19234.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig82242 2..553 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig82242.19234.1

>prot_M-pyrifera_M_contig82242.19234.1 ID=prot_M-pyrifera_M_contig82242.19234.1|Name=mRNA_M-pyrifera_M_contig82242.19234.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=184bp
LNKLLRAAGVAGKRIGVIENEAGPISIDHRLLRDAAMDPDGIFVLENGCI
CCTTGGSGDEVERVLDRAVSMMNASGEGLDHLVVETSGLADPGPLLELLF
LPHRAGALHLDGVVTLVDAKHGVQHLVESGGWGGRAVEASRQVAYADEVL
VTKVDLSPDTVGPLHKAIETLNPTTQIRNVVLVD
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mRNA from alignment at M-pyrifera_M_contig82242:2..553+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig82242.19234.1 ID=mRNA_M-pyrifera_M_contig82242.19234.1|Name=mRNA_M-pyrifera_M_contig82242.19234.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=552bp|location=Sequence derived from alignment at M-pyrifera_M_contig82242:2..553+ (Macrocystis pyrifera P11B4 male)
CTGAACAAGCTTTTACGCGCAGCTGGAGTAGCTGGGAAGCGTATTGGGGT GATTGAGAACGAGGCTGGGCCAATTTCGATCGACCATCGGTTGTTACGGG ATGCTGCAATGGATCCAGATGGGATATTTGTGCTGGAAAATGGCTGTATT TGCTGCACAACGGGTGGAAGTGGCGATGAGGTGGAGCGCGTGCTTGATCG GGCAGTGTCGATGATGAACGCAAGTGGGGAAGGATTGGACCATCTGGTGG TGGAGACCTCAGGCCTTGCAGATCCAGGGCCTCTCTTGGAACTGCTCTTT TTGCCGCATCGGGCAGGCGCATTGCATTTGGACGGTGTTGTTACCCTTGT GGACGCAAAGCACGGTGTGCAGCACTTGGTGGAGAGTGGAGGGTGGGGTG GGAGGGCAGTGGAGGCAAGTAGACAAGTCGCGTATGCAGATGAAGTCCTC GTTACCAAGGTTGACCTTTCTCCTGACACTGTTGGCCCACTTCACAAAGC AATTGAAACTCTAAATCCAACAACACAAATCAGAAACGTGGTATTGGTCG AC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig82242:2..553+

>mRNA_M-pyrifera_M_contig82242.19234.1 ID=mRNA_M-pyrifera_M_contig82242.19234.1|Name=mRNA_M-pyrifera_M_contig82242.19234.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1104bp|location=Sequence derived from alignment at M-pyrifera_M_contig82242:2..553+ (Macrocystis pyrifera P11B4 male)
CTGAACAAGCTTTTACGCGCAGCTGGAGTAGCTGGGAAGCGTATTGGGGT
GATTGAGAACGAGGCTGGGCCAATTTCGATCGACCATCGGTTGTTACGGG
ATGCTGCAATGGATCCAGATGGGATATTTGTGCTGGAAAATGGCTGTATT
TGCTGCACAACGGGTGGAAGTGGCGATGAGGTGGAGCGCGTGCTTGATCG
GGCAGTGTCGATGATGAACGCAAGTGGGGAAGGATTGGACCATCTGGTGG
TGGAGACCTCAGGCCTTGCAGATCCAGGGCCTCTCTTGGAACTGCTCTTT
TTGCCGCATCGGGCAGGCGCATTGCATTTGGACGGTGTTGTTACCCTTGT
GGACGCAAAGCACGGTGTGCAGCACTTGGTGGAGAGTGGAGGGTGGGGTG
GGAGGGCAGTGGAGGCAAGTAGACAAGTCGCGTATGCAGATGAAGTCCTC
GTTACCAAGGTTGACCTTTCTCCTGACACTGTTGGCCCACTTCACAAAGC
AATTGAAACTCTAAATCCAACAACACAAATCAGAAACGTGGTATTGGTCG
ACCTGAACAAGCTTTTACGCGCAGCTGGAGTAGCTGGGAAGCGTATTGGG
GTGATTGAGAACGAGGCTGGGCCAATTTCGATCGACCATCGGTTGTTACG
GGATGCTGCAATGGATCCAGATGGGATATTTGTGCTGGAAAATGGCTGTA
TTTGCTGCACAACGGGTGGAAGTGGCGATGAGGTGGAGCGCGTGCTTGAT
CGGGCAGTGTCGATGATGAACGCAAGTGGGGAAGGATTGGACCATCTGGT
GGTGGAGACCTCAGGCCTTGCAGATCCAGGGCCTCTCTTGGAACTGCTCT
TTTTGCCGCATCGGGCAGGCGCATTGCATTTGGACGGTGTTGTTACCCTT
GTGGACGCAAAGCACGGTGTGCAGCACTTGGTGGAGAGTGGAGGGTGGGG
TGGGAGGGCAGTGGAGGCAAGTAGACAAGTCGCGTATGCAGATGAAGTCC
TCGTTACCAAGGTTGACCTTTCTCCTGACACTGTTGGCCCACTTCACAAA
GCAATTGAAACTCTAAATCCAACAACACAAATCAGAAACGTGGTATTGGT
CGAC
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