mRNA_M-pyrifera_M_contig82234.19229.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig82234.19229.1
Unique NamemRNA_M-pyrifera_M_contig82234.19229.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig82234.19229.1 vs. uniprot
Match: A0A1G0G7U0_9GAMM (SSD domain-containing protein n=1 Tax=Gammaproteobacteria bacterium RBG_16_51_14 TaxID=1798265 RepID=A0A1G0G7U0_9GAMM)

HSP 1 Score: 177 bits (450), Expect = 3.430e-47
Identity = 111/307 (36.16%), Postives = 166/307 (54.07%), Query Frame = 1
Query:    1 FFRSVVLALCVACGIGLSVLYALXXXXXXXXXINLLTLLLLPLVFCVGLTTAVHLFSRRAGERWDYAYAIHHIARPAAIAAVTTALGCAAFAAAPQPVIARMGIVLPCGVLFSFVSTLLFVPSLLRLVTRGRPLPPL-RGYTPAIAPRGRRRVGMALVVLALGAAALLPRITLNPDAMFFFADDSPVIRSYARIERDLTGMLVADLVIRVVNDGDTVVDDANTAKVRHVIDELRRIPEMSAVVSGYGLQRLTRFAGPAAL--LGGTFFNADQRATRITLRIRNLGGRPYDAIAADVAAIWAAADTRG 912
            F RS V+  CV  GIG+S+   L          NLLTL+L PL+F +G+TTA+H FSRR G  WD   A     RP+ IA VT+ +GC  F  +PQ ++  MGIV P G+  +FV  ++FVP+   L+     LP L R  + A      + + + L   A+ A  LLPR+   PDA++FF DDS +I+SY  IE  L G+LV D+V+   NDG  +  + N  ++R V++  R +P ++ +++   L  L  +     +  L   + + D+   R+T R RN+ G PY     D+  +W  +  RG
Sbjct:  218 FLRSPVVVTCVMTGIGISLFCTLAFASGMDIQFNLLTLILYPLIFYMGITTAIHFFSRRDGGVWDLELAYAKTFRPSLIAMVTSVIGCCGFVFSPQTIMRDMGIVFPVGIFITFVVMMVFVPAAWCLLAGNGNLPCLPRAASSATVSSRSKGISLCLFAAAIVAVLLLPRLRTEPDAIYFFRDDSELIQSYRFIEDRLAGLLVMDMVVET-NDGTAITTEKNIVQIRGVVNNARSLPGLTTIITP--LDWLDGYREEVIMPELKQAYISGDKSKLRLTFRFRNISGSPYSGHMTDLKRLWNESAHRG 521          
BLAST of mRNA_M-pyrifera_M_contig82234.19229.1 vs. uniprot
Match: A0A7C1YYM5_9GAMM (Uncharacterized protein n=1 Tax=Sedimenticola sp. TaxID=1940285 RepID=A0A7C1YYM5_9GAMM)

HSP 1 Score: 174 bits (442), Expect = 5.080e-46
Identity = 111/273 (40.66%), Postives = 158/273 (57.88%), Query Frame = 1
Query:  109 TLLLLPLVFCVGLTTAVHLFSRRAGERW--DYAYAIHHIARPAAIAAVTTALGCAAFAAAPQPVIARMGIVLPCGVLFSFVSTLLFVPSLLRLVTRGRPLPPLRGYTPA---IAPRGRRRVGMALVVLALGAAALLPRITLNPDAMFFFADDSPVIRSYARIERDLTGMLVADLVIRVVNDGDTVVDDANTAKVRHVIDELRRIPEMSAVVSGYGLQRLTRFAGPAALLGGTFFNADQRATRITLRIRNLGGRPYDAIAADVAAIWAAADTRG 912
            TL+L PL+FCVGLTTA+H FSRR   RW  D+AYA   + RPA +A  TTA+G  AF  APQP I  MGI+LP G++ ++VS +LF P+L R ++ G  LPP R    A   + PR RR V + L   ALG+A L   I +NPDA+FFF  DS ++RSY +IE  L G++  +L+I     G +V+D     ++   +  +  +P +++  SG  L+       PA L        D+RA RI+L  RNL  + +  +  D+   WA+ +  G
Sbjct:  269 TLILFPLLFCVGLTTAIHFFSRRQNGRWTPDHAYA--RVLRPATMAMATTAIGTGAFLFAPQPAIRDMGILLPLGIVLTYVSVMLFTPALFRWISGGWRLPPARHGQAADSPLGPRLRRGVSLLLAAAALGSAFLAGNIRINPDAIFFFQPDSELVRSYRQIEDRLVGLMTVELMITAAP-GTSVLDAERRGRIESFLAAIDGMPSLTSRASGLDLR------SPAYLA------EDRRALRISLHYRNLASQDFSRVERDLRRNWASVNGEG 526          
BLAST of mRNA_M-pyrifera_M_contig82234.19229.1 vs. uniprot
Match: A0A3M1DPZ8_9GAMM (SSD domain-containing protein n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3M1DPZ8_9GAMM)

HSP 1 Score: 167 bits (424), Expect = 1.420e-43
Identity = 110/303 (36.30%), Postives = 158/303 (52.15%), Query Frame = 1
Query:    1 FFRSVVLALCVACGIGLSVLYALXXXXXXXXXINLLTLLLLPLVFCVGLTTAVHLFSRRAGERWDYAYAIHHIARPAAIAAVTTALGCAAFAAAPQPVIARMGIVLPCGVLFSFVSTLLFVPSLLRLVTRGRPLPPLRGYTPAIAPRGRRRVGMALVVLALGAAALLPRITLNPDAMFFFADDSPVIRSYARIERDLTGMLVADLVIRVVNDGDTVVDDANTAKVRHVIDELRRIPEMSAVVSGYGLQRLTRFA-GPAALLGGTFFNADQRATRITLRIRNLGGRPYDAIAADVAAIWAAADT 906
            F RS   ++ V    GL + Y L         +NL  L++ PL+FCV +T A+HLFS   G   +    +  + RPA IA +TT LG      APQ  + + G VLP G+  +F   LLF+P+  RL+  G  LPPL    PA  P  R+ VG+ L++    +   LPR+  +PDA+ FF +DSPV R Y  IE  L G+ V D VI    +G  + D  N A++   +  L R+P ++ V+ G G ++L+    GP   L   F + D R+ R+TL  R +GG  Y A+   V   W A +T
Sbjct:  207 FLRSWQASVAVLAASGLGLWYTLIWAGLLGYSLNLPMLVVFPLLFCVAVTLAIHLFSGHGGRERELPEVLPRLLRPALIAMLTTLLGSLTLLLAPQRALYQAGAVLPVGIGVTFALVLLFMPAAYRLLRGGWTLPPLVARPPASGPLSRQLVGLGLLLATAASLFALPRLQTDPDALNFFPEDSPVTRGYDYIEHRLCGLFVIDAVI-TAREG-RLTDPENGARIGRFLQRLARLPRLTTVLPGAGWRQLSDIVDGPGRQLRDAFLSDDGRSLRLTLHFREVGGDGYLAVERSVREAWQAGET 507          
BLAST of mRNA_M-pyrifera_M_contig82234.19229.1 vs. uniprot
Match: A0A2N1XP51_9GAMM (Uncharacterized protein n=1 Tax=Gammaproteobacteria bacterium HGW-Gammaproteobacteria-3 TaxID=2013799 RepID=A0A2N1XP51_9GAMM)

HSP 1 Score: 160 bits (406), Expect = 4.830e-41
Identity = 108/285 (37.89%), Postives = 152/285 (53.33%), Query Frame = 1
Query:   25 LCVACGIGLSVLYALXXXXXXXXXINLLTLLLLPLVFCVGLTTAVHLFSRRAGERWDYAYAIHHIARPAAIAAVTTALGCAAFAAAPQPVIARMGIVLPCGVLFSFVSTLLFVPSLLRLVTRGRPLPPLRGYTPAIAPRGRRRVGMALVVLALGAAALLPRITLNPDAMFFFADDSPVIRSYARIERDLTGMLVADLVIRVVNDGDTVVDDANTAKVRHVIDELRRIPEMSAVVSGYGLQRLTRFAGPAALLGGTFFNADQRATRITLRIRNLGGRPYDAIAADV 879
            L V  GI LS+++ L         + LL+L++ PLVFCVGLTTA+HLFS+     W  A A H I +PA IA +TTA+GCA+FA APQ  +A MG+VLP  V+ SF+ TL   P+L  L+ + R L   R       PR RR +   L+  A  AA L   I  NPDA +FF  DS V++SY  IE  LTG+L  +L++    D   + +      +   + +LR++P +++ VS +    LT        +  +  N      RITLR +N  G  Y    A +
Sbjct:  226 LSVFAGIALSLVFTLALAGAWGLKMTLLSLIIFPLVFCVGLTTAIHLFSKTTEGVWQAADAYHKIRKPAFIAMLTTAIGCASFAFAPQMTVADMGLVLPVAVILSFLCTLFVTPALFVLIDKNRILADSRRKQLNPLPRLRRWLSWMLLAGAAAAAYLALDIKTNPDAFYFFKPDSDVVKSYQLIENQLTGLLAIELILHS-EDRHKLTEAPLRPAIAAWLTQLRKLPGLTSAVSVFDALSLTSADNLPDDIRKSLLNEQATEARITLRFKNSAGFHYQETLAQI 509          
BLAST of mRNA_M-pyrifera_M_contig82234.19229.1 vs. uniprot
Match: A0A1G3AU24_9BACT (Uncharacterized protein n=3 Tax=unclassified Planctomycetes TaxID=473814 RepID=A0A1G3AU24_9BACT)

HSP 1 Score: 62.4 bits (150), Expect = 3.720e-7
Identity = 61/264 (23.11%), Postives = 116/264 (43.94%), Query Frame = 1
Query:    4 FRSVVLALCVACGIGLSVLYALXXXXXXXXXINLLTLLLLPLVFCVGLTTAVHLFSRRAGE-------RWDYAYAIHHIARPAAIAAVTTALGCAAFAAAPQPVIARMGIVLPCGVLFSFVSTLLFVPSLLRLV---------------TRGRPLPPLRGYTPAIAPRGRRRVGMALVVLALGAAALLPRITLNPDAMFFFADDSPVIRSYARIERDLTGMLVADLVIRVVNDGDTVVDDANTAKVRHVIDELRRIPEMSAVVS 729
            FR++   L  A  I +++++A+          N+++ +++PL+F + LTT VH+ +R   E       R      + HI  P  +  +TTA+G  +   +    +   GI +  G++ SF   +  VP +L L                T  R    + G+      +    + +  +V    A   + +     +    F ++S + RS   IE++L G+L  ++VI  VN G     +   AK+ ++ D L+ IPE++  +S
Sbjct:  248 FRNISGVLIPAITISINIIWAVGLFVIFNNKTNMVSGMIIPLIFIISLTTTVHILNRFYQEVKLTGDSRKSTLETVKHICVPCFLMCLTTAIGFLSLMVSDVTPVKITGIFMAAGIMMSFFVCITLVPCILSLFPGLMSSSATIQKDDGTSNREFRGIYGFIGRFVKKNTIYILILSLVFVGFAIYGITKTRAESNVFESFPENSEISRSTKHIEQNLMGLLPMEIVINAVNGGGVFQPEV-LAKMENLQDYLKEIPEVTKSMS 510          
BLAST of mRNA_M-pyrifera_M_contig82234.19229.1 vs. uniprot
Match: A0A7K3WLI6_9FLAO (RND family transporter n=1 Tax=Cryomorpha ignava TaxID=101383 RepID=A0A7K3WLI6_9FLAO)

HSP 1 Score: 59.3 bits (142), Expect = 3.740e-6
Identity = 59/227 (25.99%), Postives = 109/227 (48.02%), Query Frame = 1
Query:   97 INLLTLLLLPLVFCVGLTTAVHLFSRRAGERWD---YAYAIHHIARPAAIAA----VTTALGCAAFAAAPQPVIARMGIVLPCGVLFSFVSTLLFVPSLLRLVT---------RGRPLPPLRGYTPAIAPRGRRRVGMALVVLALGAAALLPRITLNPDAMFFFADDSPVIRSYARIERDLTGMLVADLVIRVVNDGDTVVDDANTAKVRHVIDELRRIPEMSAVVS 729
            I++L  +L P++F VG++ AVHL+SR   E  D    A+AIH +     +AA    +TTA+G A+      P +   G++   GV+ ++V ++  +P+ L L           +G     L G    I  R R+ V +  +V+ALG      R+ LN   +     D P+ + +A  + +  G+   +L +++    D+ +   N  K+  + + L+    + A++S
Sbjct:  253 ISILLNMLPPVIFVVGMSDAVHLYSRFLEELRDGATKAHAIHQMIFDTGLAALFTSITTAIGFASLYLTGIPALQEFGVITASGVIAAYVISITMLPAWLVLTEPPHKVINNEKGNWQRQLDGILIPII-RHRKWVFIGTIVIALGFGFAASRLELNNFILEDLKPDEPLRKDFAFFDVNFAGVRPFELGLKLKEQDDSFLTPQNIGKLNKLENYLKSTYGVHAILS 478          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig82234.19229.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
A0A1G0G7U0_9GAMM3.430e-4736.16SSD domain-containing protein n=1 Tax=Gammaproteob... [more]
A0A7C1YYM5_9GAMM5.080e-4640.66Uncharacterized protein n=1 Tax=Sedimenticola sp. ... [more]
A0A3M1DPZ8_9GAMM1.420e-4336.30SSD domain-containing protein n=1 Tax=Gammaproteob... [more]
A0A2N1XP51_9GAMM4.830e-4137.89Uncharacterized protein n=1 Tax=Gammaproteobacteri... [more]
A0A1G3AU24_9BACT3.720e-723.11Uncharacterized protein n=3 Tax=unclassified Planc... [more]
A0A7K3WLI6_9FLAO3.740e-625.99RND family transporter n=1 Tax=Cryomorpha ignava T... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig82234contigM-pyrifera_M_contig82234:3..914 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score62.4
Seed ortholog evalue4.2e-07
Seed eggNOG ortholog349124.Hhal_1526
KEGG koko:K07003
Hectar predicted targeting categorysignal anchor
EggNOG free text desc.MMPL family
EggNOG OGs1MUE1@1224,1RPIP@1236,1WXN9@135613,COG1033@1,COG1033@2
COG Functional cat.S
Best tax levelChromatiales
Best eggNOG OGNA|NA|NA
BRITEko00000
Exons1
Model size912
Cds size912
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816729.207576-CDS-M-pyrifera_M_contig82234:2..9141622816729.207576-CDS-M-pyrifera_M_contig82234:2..914Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82234 3..914 +
1692278144.3701491-CDS-M-pyrifera_M_contig82234:2..9141692278144.3701491-CDS-M-pyrifera_M_contig82234:2..914Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82234 3..914 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig82234.19229.1prot_M-pyrifera_M_contig82234.19229.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig82234 3..914 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig82234.19229.1

>prot_M-pyrifera_M_contig82234.19229.1 ID=prot_M-pyrifera_M_contig82234.19229.1|Name=mRNA_M-pyrifera_M_contig82234.19229.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=304bp
FFRSVVLALCVACGIGLSVLYALAVCMALGLTINLLTLLLLPLVFCVGLT
TAVHLFSRRAGERWDYAYAIHHIARPAAIAAVTTALGCAAFAAAPQPVIA
RMGIVLPCGVLFSFVSTLLFVPSLLRLVTRGRPLPPLRGYTPAIAPRGRR
RVGMALVVLALGAAALLPRITLNPDAMFFFADDSPVIRSYARIERDLTGM
LVADLVIRVVNDGDTVVDDANTAKVRHVIDELRRIPEMSAVVSGYGLQRL
TRFAGPAALLGGTFFNADQRATRITLRIRNLGGRPYDAIAADVAAIWAAA
DTRG
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mRNA from alignment at M-pyrifera_M_contig82234:3..914+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig82234.19229.1 ID=mRNA_M-pyrifera_M_contig82234.19229.1|Name=mRNA_M-pyrifera_M_contig82234.19229.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=912bp|location=Sequence derived from alignment at M-pyrifera_M_contig82234:3..914+ (Macrocystis pyrifera P11B4 male)
TTTTTCCGCAGCGTCGTGCTCGCGCTGTGCGTTGCATGCGGGATCGGCCT CAGCGTGCTGTATGCGCTCGCCGTGTGCATGGCGCTCGGGCTCACGATCA ATCTGCTCACGCTGTTACTGCTGCCGCTCGTGTTCTGCGTCGGCCTGACG ACGGCCGTACACCTGTTCTCGCGGCGTGCTGGTGAGCGCTGGGACTACGC TTACGCGATACATCATATCGCGCGGCCGGCCGCGATCGCGGCGGTGACGA CCGCACTCGGGTGCGCGGCGTTCGCGGCGGCGCCGCAGCCGGTTATCGCG CGCATGGGTATCGTGCTGCCGTGTGGCGTGTTGTTCAGCTTTGTTTCGAC CTTGCTGTTCGTGCCGTCGCTGTTGCGCCTCGTGACCCGGGGGCGGCCGT TGCCGCCACTGCGCGGCTACACCCCGGCGATCGCGCCGCGCGGTCGGCGT CGGGTCGGCATGGCTCTCGTCGTGCTCGCGCTCGGCGCCGCGGCGCTGTT GCCGCGAATCACGCTGAATCCCGATGCGATGTTTTTCTTCGCCGATGATT CGCCGGTGATCCGCTCCTATGCCCGGATCGAACGCGACCTGACCGGCATG CTCGTCGCCGATCTCGTGATCCGCGTCGTGAACGACGGCGACACCGTCGT CGATGACGCGAACACGGCGAAAGTGCGCCACGTGATCGACGAACTGCGCC GGATCCCGGAAATGTCCGCGGTCGTGTCGGGCTACGGACTGCAGCGGCTC ACCCGCTTCGCCGGGCCCGCCGCGCTGCTCGGCGGAACGTTCTTCAACGC CGATCAACGCGCGACCCGCATCACGCTCCGGATCCGCAATCTCGGCGGGC GGCCGTATGACGCGATCGCGGCCGACGTCGCCGCGATCTGGGCCGCCGCC GATACCCGTGGC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig82234:3..914+

>mRNA_M-pyrifera_M_contig82234.19229.1 ID=mRNA_M-pyrifera_M_contig82234.19229.1|Name=mRNA_M-pyrifera_M_contig82234.19229.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1824bp|location=Sequence derived from alignment at M-pyrifera_M_contig82234:3..914+ (Macrocystis pyrifera P11B4 male)
TTTTTCCGCAGCGTCGTGCTCGCGCTGTGCGTTGCATGCGGGATCGGCCT
CAGCGTGCTGTATGCGCTCGCCGTGTGCATGGCGCTCGGGCTCACGATCA
ATCTGCTCACGCTGTTACTGCTGCCGCTCGTGTTCTGCGTCGGCCTGACG
ACGGCCGTACACCTGTTCTCGCGGCGTGCTGGTGAGCGCTGGGACTACGC
TTACGCGATACATCATATCGCGCGGCCGGCCGCGATCGCGGCGGTGACGA
CCGCACTCGGGTGCGCGGCGTTCGCGGCGGCGCCGCAGCCGGTTATCGCG
CGCATGGGTATCGTGCTGCCGTGTGGCGTGTTGTTCAGCTTTGTTTCGAC
CTTGCTGTTCGTGCCGTCGCTGTTGCGCCTCGTGACCCGGGGGCGGCCGT
TGCCGCCACTGCGCGGCTACACCCCGGCGATCGCGCCGCGCGGTCGGCGT
CGGGTCGGCATGGCTCTCGTCGTGCTCGCGCTCGGCGCCGCGGCGCTGTT
GCCGCGAATCACGCTGAATCCCGATGCGATGTTTTTCTTCGCCGATGATT
CGCCGGTGATCCGCTCCTATGCCCGGATCGAACGCGACCTGACCGGCATG
CTCGTCGCCGATCTCGTGATCCGCGTCGTGAACGACGGCGACACCGTCGT
CGATGACGCGAACACGGCGAAAGTGCGCCACGTGATCGACGAACTGCGCC
GGATCCCGGAAATGTCCGCGGTCGTGTCGGGCTACGGACTGCAGCGGCTC
ACCCGCTTCGCCGGGCCCGCCGCGCTGCTCGGCGGAACGTTCTTCAACGC
CGATCAACGCGCGACCCGCATCACGCTCCGGATCCGCAATCTCGGCGGGC
GGCCGTATGACGCGATCGCGGCCGACGTCGCCGCGATCTGGGCCGCCGCC
GATACCCGTGGCTTTTTCCGCAGCGTCGTGCTCGCGCTGTGCGTTGCATG
CGGGATCGGCCTCAGCGTGCTGTATGCGCTCGCCGTGTGCATGGCGCTCG
GGCTCACGATCAATCTGCTCACGCTGTTACTGCTGCCGCTCGTGTTCTGC
GTCGGCCTGACGACGGCCGTACACCTGTTCTCGCGGCGTGCTGGTGAGCG
CTGGGACTACGCTTACGCGATACATCATATCGCGCGGCCGGCCGCGATCG
CGGCGGTGACGACCGCACTCGGGTGCGCGGCGTTCGCGGCGGCGCCGCAG
CCGGTTATCGCGCGCATGGGTATCGTGCTGCCGTGTGGCGTGTTGTTCAG
CTTTGTTTCGACCTTGCTGTTCGTGCCGTCGCTGTTGCGCCTCGTGACCC
GGGGGCGGCCGTTGCCGCCACTGCGCGGCTACACCCCGGCGATCGCGCCG
CGCGGTCGGCGTCGGGTCGGCATGGCTCTCGTCGTGCTCGCGCTCGGCGC
CGCGGCGCTGTTGCCGCGAATCACGCTGAATCCCGATGCGATGTTTTTCT
TCGCCGATGATTCGCCGGTGATCCGCTCCTATGCCCGGATCGAACGCGAC
CTGACCGGCATGCTCGTCGCCGATCTCGTGATCCGCGTCGTGAACGACGG
CGACACCGTCGTCGATGACGCGAACACGGCGAAAGTGCGCCACGTGATCG
ACGAACTGCGCCGGATCCCGGAAATGTCCGCGGTCGTGTCGGGCTACGGA
CTGCAGCGGCTCACCCGCTTCGCCGGGCCCGCCGCGCTGCTCGGCGGAAC
GTTCTTCAACGCCGATCAACGCGCGACCCGCATCACGCTCCGGATCCGCA
ATCTCGGCGGGCGGCCGTATGACGCGATCGCGGCCGACGTCGCCGCGATC
TGGGCCGCCGCCGATACCCGTGGC
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